KR102863070B1 - 돼지 트립신의 변이체 - Google Patents

돼지 트립신의 변이체

Info

Publication number
KR102863070B1
KR102863070B1 KR1020207026991A KR20207026991A KR102863070B1 KR 102863070 B1 KR102863070 B1 KR 102863070B1 KR 1020207026991 A KR1020207026991 A KR 1020207026991A KR 20207026991 A KR20207026991 A KR 20207026991A KR 102863070 B1 KR102863070 B1 KR 102863070B1
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KR
South Korea
Prior art keywords
ser
gly
asn
ile
val
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Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
KR1020207026991A
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English (en)
Korean (ko)
Other versions
KR20200124255A (ko
Inventor
토마스 슈틸거
세바스티안 리솜
클라우디아 펠러
안드레아스 포겔
Original Assignee
사노피-아벤티스 도이칠란트 게엠베하
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Publication of KR20200124255A publication Critical patent/KR20200124255A/ko
Application granted granted Critical
Publication of KR102863070B1 publication Critical patent/KR102863070B1/ko
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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/62—Insulins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6427—Chymotrypsins (3.4.21.1; 3.4.21.2); Trypsin (3.4.21.4)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21004—Trypsin (3.4.21.4)

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Endocrinology (AREA)
  • Diabetes (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Fodder In General (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)
  • Meat, Egg Or Seafood Products (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
KR1020207026991A 2018-02-22 2019-02-22 돼지 트립신의 변이체 Active KR102863070B1 (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP18158034 2018-02-22
EP18158034.1 2018-02-22
PCT/EP2019/054400 WO2019162423A1 (en) 2018-02-22 2019-02-22 Variants of porcine trypsin

Publications (2)

Publication Number Publication Date
KR20200124255A KR20200124255A (ko) 2020-11-02
KR102863070B1 true KR102863070B1 (ko) 2025-09-22

Family

ID=61274085

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1020207026991A Active KR102863070B1 (ko) 2018-02-22 2019-02-22 돼지 트립신의 변이체

Country Status (13)

Country Link
US (2) US11518988B2 (enExample)
EP (1) EP3755794A1 (enExample)
JP (2) JP7432516B2 (enExample)
KR (1) KR102863070B1 (enExample)
CN (1) CN111989401A (enExample)
AU (1) AU2019225972B2 (enExample)
BR (1) BR112020016954A2 (enExample)
CA (1) CA3091943A1 (enExample)
IL (1) IL276799A (enExample)
MX (1) MX2020008766A (enExample)
MY (1) MY200951A (enExample)
SG (1) SG11202008026WA (enExample)
WO (1) WO2019162423A1 (enExample)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2019162423A1 (en) 2018-02-22 2019-08-29 Sanofi-Aventis Deutschland Gmbh Variants of porcine trypsin
CN111676211B (zh) * 2020-05-15 2022-07-19 天津科技大学 一种抗自切和高比活力胰蛋白酶突变体
CN119552847B (zh) * 2025-01-21 2025-04-11 四川大学 链霉菌胰蛋白酶自剪切位点突变体及其应用

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20010022452A (ko) * 1997-07-31 2001-03-15 추후제출 플라비바이러스 감염에 대한 재조합 다이머형 엔벨롭 백신
US7622113B2 (en) * 2003-12-08 2009-11-24 The United States Of America As Represented By The Department Of Health And Human Services Monoclonal antibodies that bind or neutralize dengue virus
ATE517919T1 (de) * 2005-09-14 2011-08-15 Sanofi Aventis Deutschland Spaltung von insulinvorstufen durch eine trypsinvariante
BR112013009649A2 (pt) * 2010-10-29 2016-07-12 Merck Sharp & Dohme composição imunogênica, métodos para provocar uma resposta imune protetora em um paciente humano e para fornecer proteção imune em seres humanos contra doença, e, uso de uma composição
US9267114B2 (en) * 2012-11-07 2016-02-23 Southern Research Institute Flavivirus envelope protein mutations affecting virion disassembly
WO2019162423A1 (en) * 2018-02-22 2019-08-29 Sanofi-Aventis Deutschland Gmbh Variants of porcine trypsin

Also Published As

Publication number Publication date
RU2020130880A (ru) 2022-03-22
CA3091943A1 (en) 2019-08-29
US11952602B2 (en) 2024-04-09
BR112020016954A2 (pt) 2020-12-29
JP7699196B2 (ja) 2025-06-26
MX2020008766A (es) 2020-12-07
KR20200124255A (ko) 2020-11-02
US20200377873A1 (en) 2020-12-03
RU2020130880A3 (enExample) 2022-03-22
NZ768123A (en) 2026-02-27
SG11202008026WA (en) 2020-09-29
JP2021514194A (ja) 2021-06-10
AU2019225972A1 (en) 2020-10-15
JP2024038140A (ja) 2024-03-19
US20230303994A1 (en) 2023-09-28
AU2019225972B2 (en) 2024-10-17
WO2019162423A1 (en) 2019-08-29
MY200951A (en) 2024-01-25
CN111989401A (zh) 2020-11-24
EP3755794A1 (en) 2020-12-30
IL276799A (en) 2020-10-29
US11518988B2 (en) 2022-12-06
JP7432516B2 (ja) 2024-02-16

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