KR102834624B1 - 포스포아미다이트 활성화제 - Google Patents

포스포아미다이트 활성화제

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Publication number
KR102834624B1
KR102834624B1 KR1020227012543A KR20227012543A KR102834624B1 KR 102834624 B1 KR102834624 B1 KR 102834624B1 KR 1020227012543 A KR1020227012543 A KR 1020227012543A KR 20227012543 A KR20227012543 A KR 20227012543A KR 102834624 B1 KR102834624 B1 KR 102834624B1
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KR
South Korea
Prior art keywords
phosphoramidite
group
activator
molar concentration
piperidine
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Application number
KR1020227012543A
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English (en)
Korean (ko)
Other versions
KR20220083704A (ko
Inventor
세이호 기쿠치
기미히코 사노
Original Assignee
후지필름 와코 준야쿠 가부시키가이샤
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Publication of KR20220083704A publication Critical patent/KR20220083704A/ko
Application granted granted Critical
Publication of KR102834624B1 publication Critical patent/KR102834624B1/ko
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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H1/00—Processes for the preparation of sugar derivatives
    • C07H1/02—Phosphorylation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07F—ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
    • C07F9/00—Compounds containing elements of Groups 5 or 15 of the Periodic Table
    • C07F9/02—Phosphorus compounds
    • C07F9/547—Heterocyclic compounds, e.g. containing phosphorus as a ring hetero atom
    • C07F9/6524—Heterocyclic compounds, e.g. containing phosphorus as a ring hetero atom having four or more nitrogen atoms as the only ring hetero atoms
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07F—ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
    • C07F9/00—Compounds containing elements of Groups 5 or 15 of the Periodic Table
    • C07F9/02—Phosphorus compounds
    • C07F9/06—Phosphorus compounds without P—C bonds
    • C07F9/08—Esters of oxyacids of phosphorus
    • C07F9/09—Esters of phosphoric acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07F—ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
    • C07F9/00—Compounds containing elements of Groups 5 or 15 of the Periodic Table
    • C07F9/02—Phosphorus compounds
    • C07F9/06—Phosphorus compounds without P—C bonds
    • C07F9/16—Esters of thiophosphoric acids or thiophosphorous acids
    • C07F9/165—Esters of thiophosphoric acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H1/00—Processes for the preparation of sugar derivatives
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H23/00—Compounds containing boron, silicon or a metal, e.g. chelates or vitamin B12
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Saccharide Compounds (AREA)
KR1020227012543A 2019-10-18 2020-10-13 포스포아미다이트 활성화제 Active KR102834624B1 (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2019190698 2019-10-18
JPJP-P-2019-190698 2019-10-18
PCT/JP2020/038612 WO2021075423A1 (ja) 2019-10-18 2020-10-13 ホスホロアミダイト活性化剤

Publications (2)

Publication Number Publication Date
KR20220083704A KR20220083704A (ko) 2022-06-20
KR102834624B1 true KR102834624B1 (ko) 2025-07-15

Family

ID=75538471

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1020227012543A Active KR102834624B1 (ko) 2019-10-18 2020-10-13 포스포아미다이트 활성화제

Country Status (6)

Country Link
US (1) US12202848B2 (https=)
EP (1) EP4047004B1 (https=)
JP (1) JP7452549B2 (https=)
KR (1) KR102834624B1 (https=)
CN (1) CN114555617B (https=)
WO (1) WO2021075423A1 (https=)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116413359B (zh) * 2023-03-08 2025-09-16 杭州诺泰诺和生物医药科技有限公司 一种亚磷酰胺单体液相色谱快速分析方法
CN117233281B (zh) * 2023-09-14 2024-11-05 上海奥锐特生物科技有限公司 2-氰乙基-n,n,n’,n’-四异丙基亚磷酰二胺的检测方法
CN117233282B (zh) * 2023-09-14 2024-12-20 上海奥锐特生物科技有限公司 2-氰乙基-n,n,n’,n’-四异丙基亚磷酰二胺的检测方法

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004052908A1 (ja) 2002-12-09 2004-06-24 Mitsui Chemicals, Inc. フォスフォロアミダイトの製造方法

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5218088A (en) * 1989-11-02 1993-06-08 Purdue Research Foundation Process for preparing dithiophosphate oligonucleotide analogs via nucleoside thiophosphoramidite intermediates
AU2003269239A1 (en) * 2002-10-15 2004-05-04 Avecia Biotechnology Inc. Phosphitylation process
WO2005097817A2 (en) 2004-04-05 2005-10-20 Alnylam Pharmaceuticals, Inc. Process and reagents for oligonucleotide synthesis and purification
ES2386709T3 (es) * 2004-08-26 2012-08-27 Nippon Shinyaku Co., Ltd. Compuesto de fosforamidita y método para producir un oligo-ARN
US7339052B2 (en) 2005-02-08 2008-03-04 Honeywell International Inc. Phosphoramidite activator for oligonucleotide synthesis
IL185439A0 (en) * 2005-03-04 2008-01-06 Girindus Ag Synthesis of oligonucleotides
WO2006116476A1 (en) * 2005-04-27 2006-11-02 Sigma-Aldrich Co. Activators for oligonucleotide and phosphoramidite synthesis
JPWO2007097447A1 (ja) * 2006-02-27 2009-07-16 日本新薬株式会社 核酸保護基の脱離方法
CA2671873C (en) * 2006-12-12 2018-10-09 Brian Stephen Sproat Oligonucleotides containing high concentrations of guanine monomers
US7723528B2 (en) * 2008-06-05 2010-05-25 Am Chemicals Llc Sulfur transfer reagents for oligonucleotide synthesis
EP2750768B1 (en) * 2011-08-30 2018-10-03 Astex Pharmaceuticals, Inc. Decitabine derivative formulations
NO2718257T3 (https=) * 2014-05-30 2018-04-14
AU2016287585B2 (en) * 2015-07-02 2020-12-17 Otsuka Pharmaceutical Co., Ltd. Lyophilized pharmaceutical compositions
CN109311925B (zh) * 2016-05-12 2022-06-03 罗氏创新中心哥本哈根有限公司 立体限定的氧杂氮杂磷杂环戊烷亚磷酰胺单体与核苷或寡核苷酸的增强的偶联

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004052908A1 (ja) 2002-12-09 2004-06-24 Mitsui Chemicals, Inc. フォスフォロアミダイトの製造方法

Also Published As

Publication number Publication date
EP4047004B1 (en) 2024-03-06
US20240166674A1 (en) 2024-05-23
CN114555617B (zh) 2024-03-08
JPWO2021075423A1 (https=) 2021-04-22
KR20220083704A (ko) 2022-06-20
CN114555617A (zh) 2022-05-27
EP4047004A1 (en) 2022-08-24
WO2021075423A1 (ja) 2021-04-22
US12202848B2 (en) 2025-01-21
EP4047004A4 (en) 2023-06-14
JP7452549B2 (ja) 2024-03-19

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