JP7452549B2 - ホスホロアミダイト活性化剤 - Google Patents

ホスホロアミダイト活性化剤 Download PDF

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Publication number
JP7452549B2
JP7452549B2 JP2021552393A JP2021552393A JP7452549B2 JP 7452549 B2 JP7452549 B2 JP 7452549B2 JP 2021552393 A JP2021552393 A JP 2021552393A JP 2021552393 A JP2021552393 A JP 2021552393A JP 7452549 B2 JP7452549 B2 JP 7452549B2
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Japan
Prior art keywords
phosphoramidite
group
molar concentration
activator
phosphoramidite activator
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Japanese (ja)
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JPWO2021075423A1 (https=
Inventor
正峰 菊池
君彦 佐野
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Fujifilm Wako Pure Chemical Corp
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Fujifilm Wako Pure Chemical Corp
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07F—ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
    • C07F9/00—Compounds containing elements of Groups 5 or 15 of the Periodic Table
    • C07F9/02—Phosphorus compounds
    • C07F9/547—Heterocyclic compounds, e.g. containing phosphorus as a ring hetero atom
    • C07F9/6524—Heterocyclic compounds, e.g. containing phosphorus as a ring hetero atom having four or more nitrogen atoms as the only ring hetero atoms
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H1/00—Processes for the preparation of sugar derivatives
    • C07H1/02—Phosphorylation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07F—ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
    • C07F9/00—Compounds containing elements of Groups 5 or 15 of the Periodic Table
    • C07F9/02—Phosphorus compounds
    • C07F9/06—Phosphorus compounds without P—C bonds
    • C07F9/08—Esters of oxyacids of phosphorus
    • C07F9/09—Esters of phosphoric acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07F—ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
    • C07F9/00—Compounds containing elements of Groups 5 or 15 of the Periodic Table
    • C07F9/02—Phosphorus compounds
    • C07F9/06—Phosphorus compounds without P—C bonds
    • C07F9/16—Esters of thiophosphoric acids or thiophosphorous acids
    • C07F9/165—Esters of thiophosphoric acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H1/00—Processes for the preparation of sugar derivatives
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H23/00—Compounds containing boron, silicon or a metal, e.g. chelates or vitamin B12

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Saccharide Compounds (AREA)
JP2021552393A 2019-10-18 2020-10-13 ホスホロアミダイト活性化剤 Active JP7452549B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2019190698 2019-10-18
JP2019190698 2019-10-18
PCT/JP2020/038612 WO2021075423A1 (ja) 2019-10-18 2020-10-13 ホスホロアミダイト活性化剤

Publications (2)

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JPWO2021075423A1 JPWO2021075423A1 (https=) 2021-04-22
JP7452549B2 true JP7452549B2 (ja) 2024-03-19

Family

ID=75538471

Family Applications (1)

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JP2021552393A Active JP7452549B2 (ja) 2019-10-18 2020-10-13 ホスホロアミダイト活性化剤

Country Status (6)

Country Link
US (1) US12202848B2 (https=)
EP (1) EP4047004B1 (https=)
JP (1) JP7452549B2 (https=)
KR (1) KR102834624B1 (https=)
CN (1) CN114555617B (https=)
WO (1) WO2021075423A1 (https=)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116413359B (zh) * 2023-03-08 2025-09-16 杭州诺泰诺和生物医药科技有限公司 一种亚磷酰胺单体液相色谱快速分析方法
CN117233281B (zh) * 2023-09-14 2024-11-05 上海奥锐特生物科技有限公司 2-氰乙基-n,n,n’,n’-四异丙基亚磷酰二胺的检测方法
CN117233282B (zh) * 2023-09-14 2024-12-20 上海奥锐特生物科技有限公司 2-氰乙基-n,n,n’,n’-四异丙基亚磷酰二胺的检测方法

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2007531794A (ja) 2004-04-05 2007-11-08 アルニラム ファーマスーティカルズ インコーポレイテッド オリゴヌクレオチドの合成および精製に使用する方法および反応試薬
JP2008530092A (ja) 2005-02-08 2008-08-07 ハネウェル・インターナショナル・インコーポレーテッド オリゴヌクレオチド合成のためのホスホルアミダイト活性化剤

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5218088A (en) * 1989-11-02 1993-06-08 Purdue Research Foundation Process for preparing dithiophosphate oligonucleotide analogs via nucleoside thiophosphoramidite intermediates
AU2003269239A1 (en) * 2002-10-15 2004-05-04 Avecia Biotechnology Inc. Phosphitylation process
JPWO2004052908A1 (ja) 2002-12-09 2006-04-13 三井化学株式会社 フォスフォロアミダイトの製造方法
ES2386709T3 (es) * 2004-08-26 2012-08-27 Nippon Shinyaku Co., Ltd. Compuesto de fosforamidita y método para producir un oligo-ARN
IL185439A0 (en) * 2005-03-04 2008-01-06 Girindus Ag Synthesis of oligonucleotides
WO2006116476A1 (en) * 2005-04-27 2006-11-02 Sigma-Aldrich Co. Activators for oligonucleotide and phosphoramidite synthesis
JPWO2007097447A1 (ja) * 2006-02-27 2009-07-16 日本新薬株式会社 核酸保護基の脱離方法
CA2671873C (en) * 2006-12-12 2018-10-09 Brian Stephen Sproat Oligonucleotides containing high concentrations of guanine monomers
US7723528B2 (en) * 2008-06-05 2010-05-25 Am Chemicals Llc Sulfur transfer reagents for oligonucleotide synthesis
EP2750768B1 (en) * 2011-08-30 2018-10-03 Astex Pharmaceuticals, Inc. Decitabine derivative formulations
NO2718257T3 (https=) * 2014-05-30 2018-04-14
AU2016287585B2 (en) * 2015-07-02 2020-12-17 Otsuka Pharmaceutical Co., Ltd. Lyophilized pharmaceutical compositions
CN109311925B (zh) * 2016-05-12 2022-06-03 罗氏创新中心哥本哈根有限公司 立体限定的氧杂氮杂磷杂环戊烷亚磷酰胺单体与核苷或寡核苷酸的增强的偶联

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2007531794A (ja) 2004-04-05 2007-11-08 アルニラム ファーマスーティカルズ インコーポレイテッド オリゴヌクレオチドの合成および精製に使用する方法および反応試薬
JP2008530092A (ja) 2005-02-08 2008-08-07 ハネウェル・インターナショナル・インコーポレーテッド オリゴヌクレオチド合成のためのホスホルアミダイト活性化剤

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
J. Am. Chem. Soc.，1991年，113(10)，3972-3980，doi：10.1021/ja00010a045
Org. Proc. Res. Dev. ,，2000年，4(3)，175-181，doi：10.1021/op990086k

Also Published As

Publication number Publication date
EP4047004B1 (en) 2024-03-06
US20240166674A1 (en) 2024-05-23
CN114555617B (zh) 2024-03-08
JPWO2021075423A1 (https=) 2021-04-22
KR20220083704A (ko) 2022-06-20
CN114555617A (zh) 2022-05-27
EP4047004A1 (en) 2022-08-24
WO2021075423A1 (ja) 2021-04-22
US12202848B2 (en) 2025-01-21
EP4047004A4 (en) 2023-06-14
KR102834624B1 (ko) 2025-07-15

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