KR102736524B1 - 세포주 개발을 위한 상동 재조합 벡터의 고속생성을 위한 dna 플라스미드 - Google Patents

세포주 개발을 위한 상동 재조합 벡터의 고속생성을 위한 dna 플라스미드 Download PDF

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KR102736524B1
KR102736524B1 KR1020237035059A KR20237035059A KR102736524B1 KR 102736524 B1 KR102736524 B1 KR 102736524B1 KR 1020237035059 A KR1020237035059 A KR 1020237035059A KR 20237035059 A KR20237035059 A KR 20237035059A KR 102736524 B1 KR102736524 B1 KR 102736524B1
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오스카 엠. 페레스-릴
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템플 유니버시티-오브 더 커먼웰쓰 시스템 오브 하이어 에듀케이션
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
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    • C12N5/06—Animal cells or tissues; Human cells or tissues
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • C12N5/12—Fused cells, e.g. hybridomas
    • C12N5/16—Animal cells
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
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    • C12N2800/00—Nucleic acids vectors
    • C12N2800/80—Vectors containing sites for inducing double-stranded breaks, e.g. meganuclease restriction sites

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  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
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  • General Chemical & Material Sciences (AREA)
  • Public Health (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
KR1020237035059A 2016-11-03 2017-11-03 세포주 개발을 위한 상동 재조합 벡터의 고속생성을 위한 dna 플라스미드 Active KR102736524B1 (ko)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US201662416802P 2016-11-03 2016-11-03
US62/416,802 2016-11-03
PCT/US2017/059816 WO2018144087A2 (en) 2016-11-03 2017-11-03 Dna plasmids for the fast generation of homologous recombination vectors for cell line development
KR1020197015851A KR102590861B1 (ko) 2016-11-03 2017-11-03 세포주 개발을 위한 상동 재조합 벡터의 고속생성을 위한 dna 플라스미드

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KR20230148274A KR20230148274A (ko) 2023-10-24
KR102736524B1 true KR102736524B1 (ko) 2024-11-29

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KR1020197015851A Active KR102590861B1 (ko) 2016-11-03 2017-11-03 세포주 개발을 위한 상동 재조합 벡터의 고속생성을 위한 dna 플라스미드

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US (2) US12359222B2 (enExample)
EP (2) EP4219709A1 (enExample)
JP (1) JP7418796B2 (enExample)
KR (2) KR102736524B1 (enExample)
CN (1) CN110199020B (enExample)
CA (1) CA3081687A1 (enExample)
IL (1) IL266422A (enExample)
WO (1) WO2018144087A2 (enExample)

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EP4219709A1 (en) 2016-11-03 2023-08-02 Temple University - Of The Commonwealth System of Higher Education Dna plasmids for the fast generation of homologous recombination vectors for cell line development
CN109402172B (zh) * 2018-11-23 2021-11-26 四川农业大学 一种鸭坦布苏报告病毒的制备方法及其产品和应用
CN117904208A (zh) * 2018-12-30 2024-04-19 豪夫迈·罗氏有限公司 基于可检测标签与靶蛋白的CRISPR/Cas控制的整合而选择细胞的方法
CN111235183B (zh) * 2020-01-13 2021-07-27 东南大学 一种talen表达载体及其快速制备方法及靶基因和细胞双标记系统和应用
CN116721700B (zh) * 2023-08-08 2024-01-12 中国人民解放军军事科学院军事医学研究院 鉴定新型双链dna胞苷脱氨酶的方法、装置及应用
WO2025106834A1 (en) * 2023-11-17 2025-05-22 Temple University-Of The Commonwealth System Of Higher Education A single-plasmid system for enhanced crispr-cas9 mediated homologous recombination
WO2025216584A1 (ko) * 2024-04-11 2025-10-16 포항공과대학교 산학협력단 비천연 아미노산 도입 단백질의 효율적인 합성을 위한 유전체 가공 균주 및 세포 파쇄액 제작 방법

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Also Published As

Publication number Publication date
US20190284582A1 (en) 2019-09-19
KR102590861B1 (ko) 2023-10-18
JP2019537445A (ja) 2019-12-26
EP3535394A4 (en) 2020-05-27
EP3535394B1 (en) 2023-04-12
IL266422A (en) 2019-06-30
EP3535394A2 (en) 2019-09-11
KR20190113759A (ko) 2019-10-08
US12359222B2 (en) 2025-07-15
WO2018144087A2 (en) 2018-08-09
US10883120B2 (en) 2021-01-05
CA3081687A1 (en) 2018-08-09
CN110199020A (zh) 2019-09-03
WO2018144087A3 (en) 2018-10-25
KR20230148274A (ko) 2023-10-24
US20200002729A1 (en) 2020-01-02
EP4219709A1 (en) 2023-08-02
JP7418796B2 (ja) 2024-01-22
CN110199020B (zh) 2024-06-18

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