CA3081687A1 - Dna plasmids for the fast generation of homologous recombination vectors for cell line development - Google Patents

Dna plasmids for the fast generation of homologous recombination vectors for cell line development Download PDF

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Publication number
CA3081687A1
CA3081687A1 CA3081687A CA3081687A CA3081687A1 CA 3081687 A1 CA3081687 A1 CA 3081687A1 CA 3081687 A CA3081687 A CA 3081687A CA 3081687 A CA3081687 A CA 3081687A CA 3081687 A1 CA3081687 A1 CA 3081687A1
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cell
sequence
tag
gene
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CA3081687A
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English (en)
French (fr)
Inventor
Oscar M. Perez-Leal
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Temple Univ School of Medicine
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Temple Univ School of Medicine
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • C12N5/12—Fused cells, e.g. hybridomas
    • C12N5/16—Animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/80—Vectors containing sites for inducing double-stranded breaks, e.g. meganuclease restriction sites

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Mycology (AREA)
  • Medicinal Chemistry (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Veterinary Medicine (AREA)
  • General Physics & Mathematics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Pathology (AREA)
  • Toxicology (AREA)
  • Analytical Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Pharmacology & Pharmacy (AREA)
  • General Chemical & Material Sciences (AREA)
  • Public Health (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CA3081687A 2016-11-03 2017-11-03 Dna plasmids for the fast generation of homologous recombination vectors for cell line development Pending CA3081687A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201662416802P 2016-11-03 2016-11-03
US62/416,802 2016-11-03
PCT/US2017/059816 WO2018144087A2 (en) 2016-11-03 2017-11-03 Dna plasmids for the fast generation of homologous recombination vectors for cell line development

Publications (1)

Publication Number Publication Date
CA3081687A1 true CA3081687A1 (en) 2018-08-09

Family

ID=63040011

Family Applications (1)

Application Number Title Priority Date Filing Date
CA3081687A Pending CA3081687A1 (en) 2016-11-03 2017-11-03 Dna plasmids for the fast generation of homologous recombination vectors for cell line development

Country Status (8)

Country Link
US (2) US12359222B2 (enExample)
EP (2) EP3535394B1 (enExample)
JP (1) JP7418796B2 (enExample)
KR (2) KR102736524B1 (enExample)
CN (1) CN110199020B (enExample)
CA (1) CA3081687A1 (enExample)
IL (1) IL266422A (enExample)
WO (1) WO2018144087A2 (enExample)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2018144087A2 (en) 2016-11-03 2018-08-09 Temple University-Of The Commonwealth System Of Higher Education Dna plasmids for the fast generation of homologous recombination vectors for cell line development
CN109402172B (zh) * 2018-11-23 2021-11-26 四川农业大学 一种鸭坦布苏报告病毒的制备方法及其产品和应用
JP7233545B2 (ja) * 2018-12-30 2023-03-06 エフ． ホフマン－ラ ロシュ アーゲー 標的タンパク質への検出可能なタグのＣＲＩＳＰＲ／Ｃａｓ制御組み込みに基づく細胞の選択方法
CN111235183B (zh) * 2020-01-13 2021-07-27 东南大学 一种talen表达载体及其快速制备方法及靶基因和细胞双标记系统和应用
CN116721700B (zh) * 2023-08-08 2024-01-12 中国人民解放军军事科学院军事医学研究院 鉴定新型双链dna胞苷脱氨酶的方法、装置及应用
WO2025106834A1 (en) * 2023-11-17 2025-05-22 Temple University-Of The Commonwealth System Of Higher Education A single-plasmid system for enhanced crispr-cas9 mediated homologous recombination

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US4937190A (en) 1987-10-15 1990-06-26 Wisconsin Alumni Research Foundation Translation enhancer
US5464764A (en) * 1989-08-22 1995-11-07 University Of Utah Research Foundation Positive-negative selection methods and vectors
IL102404A0 (en) 1992-07-03 1993-01-14 Daniel Zurr Method and means for the production of gene products
DK0695361T4 (da) 1993-04-21 2010-03-22 Univ Edinburgh Ekspression af heterologe gener ifølge en målekspressionsprofil
FR2722208B1 (fr) 1994-07-05 1996-10-04 Inst Nat Sante Rech Med Nouveau site interne d'entree des ribosomes, vecteur le contenant et utilisation therapeutique
US5989904A (en) 1994-10-11 1999-11-23 The Regents Of The University Of California Selective inhibition of internally initiated RNA translation
EP1015620B1 (en) * 1997-09-23 2005-08-31 Genvec, Inc. Dual selection cassette and plasmids containing same
ATE443074T1 (de) * 1999-03-02 2009-10-15 Life Technologies Corp Zubereitungen und methoden zur verwendung in rekombinatorischem klonen von nukleinsäuren
JP5442177B2 (ja) * 2001-02-23 2014-03-12 ユニベルスィテ リーブル ドゥ ブリュッセル 毒性分子に対する抗毒性蛋白質をコードする配列を含む組換体クローンの選択方法
EP1428885A4 (en) * 2001-08-28 2006-08-09 Japan Tobacco Inc METHOD FOR MODIFYING THE GENOME IN A HIGHER PLANT
EP1686998A1 (en) * 2003-10-31 2006-08-09 Pfizer Products Inc. Phosphodiesterase 9 inhibition as treatment for obesity-related conditions
JP2007124915A (ja) * 2005-11-01 2007-05-24 Tokai Univ リコンビニアリングコンストラクト、及びジーンターゲティングコンストラクト作製用ベクター
JP2008253254A (ja) * 2007-03-14 2008-10-23 National Institutes Of Natural Sciences 高効率変異導入法
US20090111099A1 (en) * 2007-10-27 2009-04-30 Yongsheng Ma Promoter Detection and Analysis
WO2010009735A2 (en) * 2008-07-23 2010-01-28 Dako Denmark A/S Combinatorial analysis and repair
CA2732045A1 (en) 2008-08-04 2010-02-11 Schering Corporation Urea derivatives as antibacterial agents
WO2011101696A1 (en) * 2010-02-18 2011-08-25 Cellectis Improved meganuclease recombination system
EP3450563A1 (en) * 2011-02-25 2019-03-06 Recombinetics, Inc. Genetically modified animals and methods for making the same
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JP6374176B2 (ja) * 2014-02-13 2018-08-15 学校法人 名城大学 植物体に用いる組換えベクター及びその利用
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WO2018144087A2 (en) 2016-11-03 2018-08-09 Temple University-Of The Commonwealth System Of Higher Education Dna plasmids for the fast generation of homologous recombination vectors for cell line development

Also Published As

Publication number Publication date
KR20190113759A (ko) 2019-10-08
JP7418796B2 (ja) 2024-01-22
US12359222B2 (en) 2025-07-15
EP3535394A4 (en) 2020-05-27
US10883120B2 (en) 2021-01-05
CN110199020A (zh) 2019-09-03
WO2018144087A3 (en) 2018-10-25
EP3535394A2 (en) 2019-09-11
CN110199020B (zh) 2024-06-18
KR102590861B1 (ko) 2023-10-18
WO2018144087A2 (en) 2018-08-09
US20200002729A1 (en) 2020-01-02
EP4219709A1 (en) 2023-08-02
JP2019537445A (ja) 2019-12-26
US20190284582A1 (en) 2019-09-19
KR102736524B1 (ko) 2024-11-29
EP3535394B1 (en) 2023-04-12
KR20230148274A (ko) 2023-10-24
IL266422A (en) 2019-06-30

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