CN110199020B - 用于快速产生用于细胞系开发的同源重组载体的dna质粒 - Google Patents

用于快速产生用于细胞系开发的同源重组载体的dna质粒 Download PDF

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CN110199020B
CN110199020B CN201780082232.8A CN201780082232A CN110199020B CN 110199020 B CN110199020 B CN 110199020B CN 201780082232 A CN201780082232 A CN 201780082232A CN 110199020 B CN110199020 B CN 110199020B
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CN110199020A (zh
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奥斯卡·M·佩雷斯-莱亚尔
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Temple Univ School of Medicine
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    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
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    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
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    • C12N2800/00—Nucleic acids vectors
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CN201780082232.8A 2016-11-03 2017-11-03 用于快速产生用于细胞系开发的同源重组载体的dna质粒 Active CN110199020B (zh)

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US201662416802P 2016-11-03 2016-11-03
US62/416,802 2016-11-03
PCT/US2017/059816 WO2018144087A2 (en) 2016-11-03 2017-11-03 Dna plasmids for the fast generation of homologous recombination vectors for cell line development

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CN110199020B true CN110199020B (zh) 2024-06-18

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US (2) US12359222B2 (enExample)
EP (2) EP4219709A1 (enExample)
JP (1) JP7418796B2 (enExample)
KR (2) KR102736524B1 (enExample)
CN (1) CN110199020B (enExample)
CA (1) CA3081687A1 (enExample)
IL (1) IL266422A (enExample)
WO (1) WO2018144087A2 (enExample)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP4219709A1 (en) 2016-11-03 2023-08-02 Temple University - Of The Commonwealth System of Higher Education Dna plasmids for the fast generation of homologous recombination vectors for cell line development
CN109402172B (zh) * 2018-11-23 2021-11-26 四川农业大学 一种鸭坦布苏报告病毒的制备方法及其产品和应用
CN117904208A (zh) * 2018-12-30 2024-04-19 豪夫迈·罗氏有限公司 基于可检测标签与靶蛋白的CRISPR/Cas控制的整合而选择细胞的方法
CN111235183B (zh) * 2020-01-13 2021-07-27 东南大学 一种talen表达载体及其快速制备方法及靶基因和细胞双标记系统和应用
CN116721700B (zh) * 2023-08-08 2024-01-12 中国人民解放军军事科学院军事医学研究院 鉴定新型双链dna胞苷脱氨酶的方法、装置及应用
WO2025106834A1 (en) * 2023-11-17 2025-05-22 Temple University-Of The Commonwealth System Of Higher Education A single-plasmid system for enhanced crispr-cas9 mediated homologous recombination
WO2025216584A1 (ko) * 2024-04-11 2025-10-16 포항공과대학교 산학협력단 비천연 아미노산 도입 단백질의 효율적인 합성을 위한 유전체 가공 균주 및 세포 파쇄액 제작 방법

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EP3078676A1 (en) * 2015-04-10 2016-10-12 Ares Trading S.A. Induction dosing regimen for the treatment of tnf alpha mediated disorders

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FR2722208B1 (fr) 1994-07-05 1996-10-04 Inst Nat Sante Rech Med Nouveau site interne d'entree des ribosomes, vecteur le contenant et utilisation therapeutique
ATE276269T1 (de) 1994-10-11 2004-10-15 Univ California Selektive inhibierung von intern initiierter rna- translation
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Publication number Publication date
US20190284582A1 (en) 2019-09-19
KR102590861B1 (ko) 2023-10-18
JP2019537445A (ja) 2019-12-26
EP3535394A4 (en) 2020-05-27
KR102736524B1 (ko) 2024-11-29
EP3535394B1 (en) 2023-04-12
IL266422A (en) 2019-06-30
EP3535394A2 (en) 2019-09-11
KR20190113759A (ko) 2019-10-08
US12359222B2 (en) 2025-07-15
WO2018144087A2 (en) 2018-08-09
US10883120B2 (en) 2021-01-05
CA3081687A1 (en) 2018-08-09
CN110199020A (zh) 2019-09-03
WO2018144087A3 (en) 2018-10-25
KR20230148274A (ko) 2023-10-24
US20200002729A1 (en) 2020-01-02
EP4219709A1 (en) 2023-08-02
JP7418796B2 (ja) 2024-01-22

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