CN110199020B - 用于快速产生用于细胞系开发的同源重组载体的dna质粒 - Google Patents

用于快速产生用于细胞系开发的同源重组载体的dna质粒 Download PDF

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CN110199020B
CN110199020B CN201780082232.8A CN201780082232A CN110199020B CN 110199020 B CN110199020 B CN 110199020B CN 201780082232 A CN201780082232 A CN 201780082232A CN 110199020 B CN110199020 B CN 110199020B
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CN110199020A (zh
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奥斯卡·M·佩雷斯-莱亚尔
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Temple Univ School of Medicine
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
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    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
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CN201780082232.8A 2016-11-03 2017-11-03 用于快速产生用于细胞系开发的同源重组载体的dna质粒 Active CN110199020B (zh)

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US201662416802P 2016-11-03 2016-11-03
US62/416,802 2016-11-03
PCT/US2017/059816 WO2018144087A2 (en) 2016-11-03 2017-11-03 Dna plasmids for the fast generation of homologous recombination vectors for cell line development

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CN110199020B true CN110199020B (zh) 2024-06-18

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US (2) US12359222B2 (enExample)
EP (2) EP3535394B1 (enExample)
JP (1) JP7418796B2 (enExample)
KR (2) KR102736524B1 (enExample)
CN (1) CN110199020B (enExample)
CA (1) CA3081687A1 (enExample)
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Families Citing this family (6)

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Publication number Priority date Publication date Assignee Title
WO2018144087A2 (en) 2016-11-03 2018-08-09 Temple University-Of The Commonwealth System Of Higher Education Dna plasmids for the fast generation of homologous recombination vectors for cell line development
CN109402172B (zh) * 2018-11-23 2021-11-26 四川农业大学 一种鸭坦布苏报告病毒的制备方法及其产品和应用
JP7233545B2 (ja) * 2018-12-30 2023-03-06 エフ． ホフマン－ラ ロシュ アーゲー 標的タンパク質への検出可能なタグのＣＲＩＳＰＲ／Ｃａｓ制御組み込みに基づく細胞の選択方法
CN111235183B (zh) * 2020-01-13 2021-07-27 东南大学 一种talen表达载体及其快速制备方法及靶基因和细胞双标记系统和应用
CN116721700B (zh) * 2023-08-08 2024-01-12 中国人民解放军军事科学院军事医学研究院 鉴定新型双链dna胞苷脱氨酶的方法、装置及应用
WO2025106834A1 (en) * 2023-11-17 2025-05-22 Temple University-Of The Commonwealth System Of Higher Education A single-plasmid system for enhanced crispr-cas9 mediated homologous recombination

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EP3078676A1 (en) * 2015-04-10 2016-10-12 Ares Trading S.A. Induction dosing regimen for the treatment of tnf alpha mediated disorders

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FR2722208B1 (fr) 1994-07-05 1996-10-04 Inst Nat Sante Rech Med Nouveau site interne d'entree des ribosomes, vecteur le contenant et utilisation therapeutique
US5989904A (en) 1994-10-11 1999-11-23 The Regents Of The University Of California Selective inhibition of internally initiated RNA translation
EP1015620B1 (en) * 1997-09-23 2005-08-31 Genvec, Inc. Dual selection cassette and plasmids containing same
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JP5442177B2 (ja) * 2001-02-23 2014-03-12 ユニベルスィテ リーブル ドゥ ブリュッセル 毒性分子に対する抗毒性蛋白質をコードする配列を含む組換体クローンの選択方法
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KR20190113759A (ko) 2019-10-08
JP7418796B2 (ja) 2024-01-22
CA3081687A1 (en) 2018-08-09
US12359222B2 (en) 2025-07-15
EP3535394A4 (en) 2020-05-27
US10883120B2 (en) 2021-01-05
CN110199020A (zh) 2019-09-03
WO2018144087A3 (en) 2018-10-25
EP3535394A2 (en) 2019-09-11
KR102590861B1 (ko) 2023-10-18
WO2018144087A2 (en) 2018-08-09
US20200002729A1 (en) 2020-01-02
EP4219709A1 (en) 2023-08-02
JP2019537445A (ja) 2019-12-26
US20190284582A1 (en) 2019-09-19
KR102736524B1 (ko) 2024-11-29
EP3535394B1 (en) 2023-04-12
KR20230148274A (ko) 2023-10-24
IL266422A (en) 2019-06-30

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