KR102672429B1 - Manufacturing method of ink composition for functional scalp coloring - Google Patents

Abstract

The present invention relates to a method for producing an ink composition for functional scalp coloring, and more specifically, to a method for producing an ink composition for scalp coloring containing as an active ingredient a natural complex extract mixed with black ginger extract, green tea extract, or Ecklonia cava extract. It's about. The ink composition for functional scalp coloring prepared by the manufacturing method of the present invention exhibits excellent coloring power, does not irritate the skin, has a fast drying speed, has the effect of maintaining vivid color for a long time, and is suitable for those with oily and thick skin. It is effective in gaining export competitiveness because it has excellent safety and coloring ability even for Southeast Asian products.

Description

Manufacturing method of ink composition for functional scalp coloring}

The present invention relates to a method of manufacturing an ink composition for functional scalp coloring, which contains black ginger extract, green tea extract, or Ecklonia cava extract as active ingredients to alleviate skin trouble problems and ensure stable tattooing even for people with oily and thick scalp layers. It relates to a method of producing an ink composition for maintaining scalp coloring.

Among the natural extracts used in the ink composition for scalp coloring of the present invention, black ginger ( Kaempferia parviflora ) is an annual herbaceous plant of the ginger family. Its origin is the tropical regions of Southeast Asia and South Asia, and its arginine content is 11 times higher than that of regular ginger and 3.6 times higher than that of oysters, which are famous as a natural tonic. It is known that saponin is 5 times that of ginseng, anthocyanin is 3 times that of blueberries, and 17 times that of strawberries. Additionally, green tea ( Camellia sinensis ) refers to unfermented tea leaves that have been dried to a bluish color, or water steeped in tea leaves. Green tea is known to lower cholesterol levels, break down fat, prevent the formation of blood clots, lower cholesterol and blood sugar levels, prevent tooth decay caused by caries bacteria, and be effective in relieving bad breath. Lastly, Ecklonia cava is a brown algae of the seaweed family of the brown algae plant Kelp order and is known to be effective in preventing aging, bone health, anti-cancer effects, and diabetes prevention.

Meanwhile, with the increase in exports of cultural industries, including popular music, dramas, and entertainment, the world's interest in Korean celebrities and their appearances has increased significantly. This means that the Korean beauty industry, so-called K-beauty, is recognized globally, and especially in East Asia, the demand for both men and women to learn Korean makeup methods and purchase Korean cosmetics is increasing significantly. Meanwhile, interest in beauty products is also growing among people with thin or sparse hair. Existing hair loss medications or hair loss shampoos have limitations in that they are ineffective, so alternative elements such as hair transplants, wigs, or black hair are being used.

However, the hairline is a part of biological tissue made up of hair, and there are differences in hair location, hair thickness, or hair thickness depending on each individual's biological characteristics, and in particular, problems such as a gradual decrease in the number of hair or thinning hair occur as one ages. As a result, it is very difficult to continuously maintain a beautiful hairline and abundant hair. When doing hair makeup, shading and eye shadow, rather than hair products, are used to fill in empty areas of the hairline to maintain the hairline. However, such products have very short lasting properties and have problems such as smearing. Accordingly, the number of cases of scalp tattooing on part of the scalp, such as the hairline, or on the entire scalp is increasing. Scalp tattoos are gaining popularity because they last for a long time and are not difficult to maintain.

However, in the case of existing tattoo colorants, interest is only given to color and durability, and skin safety aspects are not considered much. In addition, the development of colorants tailored to individual skin conditions is also slow. Therefore, in the present invention, an ink composition for scalp coloring was invented by confirming the effect of alleviating skin troubles and stably maintaining tattoos even in people with oily and thick scalp layers by including black ginger extract, green tea extract, or Ecklonia cava extract as active ingredients. was completed.

10-2022-0044002

One object of the present invention to solve the above problems is to provide a method for producing a functional scalp coloring ink composition that can alleviate skin troubles even after tattooing and has excellent durability.

Another object of the present invention is to provide a method for producing a functional ink composition for coloring the scalp that can be applied even to people with oily or thick skin.

In order to achieve the above object, the present invention extracts black ginger powder, green tea leaf powder, and Ecklonia cava powder using isopropyl alcohol as a solvent, filters, concentrates, and freeze-dries to produce each extract. Step 1 of manufacturing;

Step 2 of preparing a natural complex extract by mixing the black ginger extract, green tea leaf extract, and Ecklonia cava extract prepared in Step 1 above at a weight ratio of 2:1:1;

Prepare ink for scalp coloring by mixing 1 to 5% by weight of the natural mixed extract mixed in step 2, 5 to 10% by weight of isopropyl alcohol, 12 to 17% by weight of inorganic pigment, and 70 to 75% by weight of distilled water for injection. In how to do it,

The ink for scalp coloring prepared by the above method has excellent coloring power and coloring persistence, does not cause skin irritation, has an effect on skin inflammation, and has an activity to inhibit nitric oxide (NO) production. Provides a method for manufacturing.

The composition prepared by the manufacturing method of the ink composition for functional scalp coloring of the present invention exhibits excellent coloring power, does not irritate the skin, dries quickly, and can maintain vivid color for a long time. In addition, we can provide an ink composition for scalp coloring with excellent safety and coloring power to Southeast Asians with oily and thick skin, which has the effect of gaining export competitiveness.

Figure 1 is a diagram showing a procedure being performed on a tester using the ink composition for scalp coloring of the present invention, in one embodiment of the present invention.
Figure 2 is a diagram showing before and after the procedure using the ink composition for scalp coloring of the present invention in one embodiment of the present invention.
Figure 3 is a diagram showing the results of three repeated treatments using the ink composition for scalp coloring of the present invention in one embodiment of the present invention.

Hereinafter, the present invention will be described in detail through embodiments of the present invention with reference to the attached drawings. However, the following implementation examples are provided as examples of the present invention, and if it is judged that a detailed description of a technology or configuration well known to those skilled in the art may unnecessarily obscure the gist of the present invention, the detailed description may be omitted. , the present invention is not limited thereby. The present invention is capable of various modifications and applications within the description of the patent claims described below and the scope of equivalents interpreted therefrom.

In addition, terminology used in this specification is a term used to appropriately express preferred embodiments of the present invention, and may vary depending on the intention of the user or operator or the customs of the field to which the present invention belongs. Therefore, definitions of these terms should be made based on the content throughout this specification. Throughout the specification, when a part is said to “include” a certain element, this means that it may further include other elements rather than excluding other elements, unless specifically stated to the contrary.

Throughout this specification, '%' used to indicate the concentration of a specific substance means (w/w) % for solid/solid, (w/v) % for solid/liquid, and Liquid/liquid is (v/v) %.

The present invention relates to an ink composition for coloring the scalp containing black ginger extract, isopropyl alcohol, inorganic pigment, and distilled water for injection, and provides a long-term coloring effect after one use.

The present invention uses natural extracts, inorganic pigments, and isopropyl alcohol that do not irritate the skin, and has the ability to quickly absorb and not stain the area where hair color is desired.

In addition, the ink composition for scalp coloring of the present invention contains black ginger extract, which is effective in improving skin inflammation. In this case, when green tea leaf extract and Ecklonia cava extract are mixed and used, the effect of improving skin inflammation is further increased.

When using a mixture of black ginger extract, green tea leaf extract, and Ecklonia cava extract in the ink composition for scalp coloring of the present invention, the skin inflammation improvement effect is most increased when mixed at a weight ratio of 2:1:1.

Hereinafter, embodiments of the present invention will be described in more detail with reference to the attached drawings. However, the following examples are only intended to embody the content of the present invention, and the present invention is not limited thereto.

<Preparation Example 1> Preparation of natural extract

1-1. Preparation of thermal water extract

100 g each of commercially available black ginger powder, green tea leaf powder, and Ecklonia cava powder were extracted with 500 ml of distilled water at 100°C for 2 hours. After extraction, it was filtered through a 5μm filter, concentrated in a vacuum concentrator, and then frozen and dried to prepare each hot water extraction powder, which was named Preparation Examples 1-1-1, 1-1-2, and 1-1-3, respectively. did.

1-2. Preparation of ethanol extract

100 g each of commercially available black ginger powder, green tea leaf powder, and Ecklonia cava powder were extracted with 500 ml of 70% ethanol for 2 hours at 25°C. After extraction, it was filtered through a 5μm filter, concentrated in a vacuum concentrator, and then frozen and dried to prepare ethanol extract powders, which were prepared as Preparation Examples 1-2-1, 1-2-2, and 1-2-3, respectively. It was named.

1-3. Preparation of propanol extract

100 g each of commercially available black ginger powder, green tea leaf powder, and Ecklonia cava powder were extracted with 70% propanol in 500 ml for 2 hours at 25°C. After extraction, it was filtered through a 5μm filter, concentrated in a vacuum concentrator, and then frozen and dried to prepare propanol extracted powders, which were named Preparation Examples 1-3-1, 1-3-2, and 1-3-3, respectively. did.

1-4. Preparation of isopropyl alcohol (propan-2-ol) extract

100 g each of commercially available black ginger powder, green tea leaf powder, and Ecklonia cava powder were extracted with 500 ml of 70% isopropyl alcohol at 25°C for 2 hours. After extraction, it was filtered through a 5μm filter, concentrated in a vacuum concentrator, and then frozen and dried to prepare isopropyl alcohol extracted powders, which were prepared in Preparation Examples 1-4-1, 1-4-2, and 1-4-3, respectively. It was named.

1-5. Preparation of butanol extract

100 g each of commercially available black ginger powder, green tea leaf powder, and Ecklonia cava powder were extracted with 70% butanol in 500 ml for 2 hours at 25°C. After extraction, it was filtered through a 5μm filter, concentrated in a vacuum concentrator, and then frozen and dried to prepare butanol extracted powders, which were named Preparation Examples 1-5-1, 1-5-2, and 1-5-3, respectively. did.

The prepared extracts are summarized in Table 1 below.

thermal water extraction Ethanol Extraction Propanol extraction Isopropyl alcohol extraction Butanol extraction black ginger 1-1-1 1-2-1 1-3-1 1-4-1 1-5-1 green tea leaves 1-1-2 1-2-2 1-3-2 1-4-2 1-5-2 Gamtae 1-1-3 1-2-3 1-3-3 1-4-3 1-5-3

<Preparation Example 2> Preparation of ink composition for scalp coloring

Preparation Examples 1-1-1, 1-1-2, 1-1-3, 1-2-1, 1-2-2, 1-2-3, 1-3-1 prepared in Preparation Example 1 , 1-3-2, 1-3-3, 1-4-1, 1-4-2, 1-4-3, 1-5-1, 1-5-2 and 1-5-3 for a total of 15 30% by weight of each extract powder was dissolved in the solvent used in preparing each extract powder and named as extraction powder + solvent (using the same solvent as the extraction solvent). Thereafter, an ink composition for scalp coloring was prepared according to Table 2 below. At this time, carbon black CI77266 (CAS: 1333-86-4) was used as the inorganic pigment.

Composition of ink composition for scalp coloring weight % Distilled water 75% Inorganic pigment (carbon black CI77266) 15% Extraction powder + each solvent (3:7) 10%

Each extracted powder (Production Examples 1-1-1, 1-1-2, 1-1-3, 1-2-1, 1-2-2, 1-2-3, 1-3-1, 1 -3-2, 1-3-3, 1-4-1, 1-4-2, 1-4-3, 1-5-1, 1-5-2 and 1-5-3) An ink composition for scalp coloring was prepared, and it was prepared in Preparation Examples 2-1, 2-2, 2-3, 2-4, 2-5, 2-6, 2-7, 2-8, 2-9, and 2, respectively. They were named -10, 2-11, 2-12, 2-13, 2-14 and 2-15.

<Experimental Example 1> Confirmation of the inflammation-improving effect of natural extracts

Preparation examples 1-1-1, 1-1-2, 1-1-3, 1-2-1, 1-2-2, 1-2-3, 1-3-1 prepared in Preparation Example 1 above , extracts of 1-3-2, 1-3-3, 1-4-1, 1-4-2, 1-4-3, 1-5-1, 1-5-2 and 1-5-3 To evaluate the anti-inflammatory effect, the nitric oxide (NO) production inhibitory activity was measured. The amount of NO was confirmed by measuring the amount of nitrite, a stable oxidation product. Specifically, RAW 264.7 macrophages (1x10 ⁴ cells/well) were cultured using DMEM (10% FBS, 1% penicillin-streptomycin) at 37°C for 12-18 hours in a 96-well plate, and then incubated at 1 ㎍/㎖ LPS was treated. Thereafter, culture was performed in medium containing 10% (w/v) of each extract for 24 hours, and 100 μl of the cell culture supernatant was incubated in a new 96-well plate with Greek reagent (Sigma-Aldrich Co., St. Louis, MO, After mixing with 100 ㎕ of USA), the absorbance was measured at 550 nm. Nitrite concentration was determined by comparison to a sodium nitrite standard curve. Ascorbic acid was used as a positive control. The results of nitric oxide (NO) production inhibition activity according to each preparation example are shown in Table 3 below. At this time, the inhibitory activity of nitric oxide (NO) production of ascorbic acid in the positive control group was found to be 83.2%.

Inhibition rate%
(black ginger) 1-1-1
(thermal water) 1-2-1
(ethanol) 1-3-1
(Propanol) 1-4-1
(isopropyl alcohol) 1-5-1
(butanol) 6.4% 18.7% 31.1% 42.2% 27.4% Inhibition rate%
(green tea leaves) 1-1-2
(thermal water) 1-2-2
(ethanol) 1-3-2
(Propanol) 1-4-2
(isopropyl alcohol) 1-5-2
(butanol) 4.2% 7.9% 17.8% 30.4% 6.8% Inhibition rate%
(Eamtae) 1-1-3
(thermal water) 1-2-3
(ethanol) 1-3-3
(Propanol) 1-4-3
(isopropyl alcohol) 1-5-3
(butanol) 5.7% 29.2% 14.4% 20.8% 11.8%

As shown in Table 3 above, the extract obtained by extracting black ginger with isopropyl alcohol showed the highest nitric oxide (NO) production inhibition activity at 42.2%, the extract obtained by extracting green tea leaves with isopropyl alcohol showed the highest activity in inhibiting nitric oxide (NO) production at 30.4%, and the extract obtained by extracting Ecklonia cava with ethanol showed the highest activity in inhibiting nitric oxide (NO) production. In this order, 29.2% showed nitric oxide (NO) production inhibitory activity. Therefore, the following experiment was conducted to determine whether there was a synergistic anti-inflammatory effect by mixing extracts with good inflammation-improving effects.

<Experimental Example 2> Confirmation of inflammation improvement effect of natural complex extract

The effect of improving inflammation was confirmed by mixing 1-4-1 (black ginger), 1-4-2 (green tea leaf), and 1-2-3 (Ecklonia cava), which were effective extracts confirmed in Experimental Example 1. The experimental method was conducted in the same manner as in Experimental Example 1, and 1-4-1 (black ginger), 1-4-2 (green tea leaves), and 1-2-3 (Ecklonia cava) were mixed at a weight ratio of 1:1:1. , mixed at a weight ratio of 2:1:1, mixed at a weight ratio of 1:2:1, and mixed at a weight ratio of 1:1:2 to confirm the effect of improving inflammation (see Table 4).

Inhibition rate%
(black ginger: green tea leaves: gamtae) 1:1:1 2:1:1 1:2:1 1:1:2 34.4% 58.8% 37.0% 41.2%

As shown in Table 4 above, when the extract obtained by extracting black ginger with isopropyl alcohol, the extract obtained by extracting green tea leaves with isopropyl alcohol, and the extract obtained by extracting Ecklonia cava with ethanol at a weight ratio of 2:1:1, the oxidation was significantly higher at 58.8%. The activity of suppressing nitrogen (NO) production was confirmed, and in other experimental groups, it was confirmed that the extract obtained from black ginger with isopropyl alcohol showed a lower anti-inflammatory effect than when used alone. Therefore, as described above, a composite extract obtained by mixing the extract obtained by extracting black ginger with isopropyl alcohol, the extract obtained by extracting green tea leaves with isopropyl alcohol, and the extract obtained by extracting Ecklonia cava with ethanol in a ratio of 2:1:1 was prepared according to the method of Preparation Example 2 above. An ink composition for scalp coloring was prepared, and it was named Preparation Example 2-16 (dissolved in isopropyl alcohol).

<Experimental Example 3> Test of coloring power of ink composition for scalp coloring

After testing each composition according to Preparation Examples 2-1 to 2-16 on the hairline scalp, the degree of coloring was observed with the naked eye and evaluated using a 10-point scale dividing 1 to 10 points depending on the degree of coloring, The scores were 9 to 10 points: very excellent coloring, 7 to 8 points: good, 5 to 6: average, 3 to 4 points: slight coloration, and 1 to 2 points: no coloration. As a result, the results are as follows. It is shown in Table 5.

Tinting power 2-1 2-2 2-3 2-4 2-5 2-6 4 5 4 5 5 6 Tinting power 2-7 2-8 2-9 2-10 2-11 2-12 6 5 6 10 9 9 Tinting power 2-13 2-14 2-15 2-16 7 6 6 10

As can be seen from the results in Table 5 above, it was confirmed that when isopropyl alcohol was used as the solvent (2-10, 2-11, 2-12 and 2-16), the degree of coloring of the hairline scalp was the best. Next, we tested the degree of maintenance of coloring power after coloring. Figures 1 and 2 show the coloring procedure and results using the composition of Preparation Example 2-16.

<Experimental Example 4> Test for maintaining coloring power of ink composition for scalp coloring

Each composition according to Preparation Examples 2-1 to 2-16 was tested on the hairline scalp, and the degree of coloring was observed with the naked eye after 1, 3, and 7 days. Depending on the degree of coloring, 1 to 10 This was evaluated using a 10-point scale divided into points, with scores of 9 to 10: Coloration remains the same, 7 to 8 points: Coloration has become slightly lighter, but there is no need for reinforcement, 5 to 6: Color has faded, 3 to 4. Points: The color was faded and a need for reinforcement was felt. 1 to 2 points: This was indicated as showing the original skin color as no coloring remained. The results are shown in Table 6 below.

Tinting power Color retention 1 day has passed 3 days have passed 7 days have passed Manufacturing Example 2-1 4 4 3 3 Manufacturing Example 2-2 5 4 3 3 Manufacturing Example 2-3 4 4 4 3 Manufacturing Example 2-4 5 5 5 4 Manufacturing Example 2-5 5 4 4 3 Manufacturing Example 2-6 6 5 5 4 Manufacturing Example 2-7 6 5 5 5 Manufacturing Example 2-8 5 5 4 4 Manufacturing Example 2-9 6 6 5 4 Manufacturing Example 2-10 10 10 9 9 Manufacturing Example 2-11 9 9 8 8 Manufacturing Example 2-12 9 9 8 8 Manufacturing Example 2-13 7 7 6 5 Manufacturing Example 2-14 6 5 5 4 Manufacturing Example 2-15 6 5 5 4 Manufacturing Example 2-16 10 10 10 9

<Experimental Example 5> Skin irritation test

The compositions according to Preparation Examples 2-10, 2-11, 2-12, and 2-16, which were effective in Experimental Examples 1 to 3, were applied between the shoulder blades and the waist line centered on the spine, and applied to the skin for 48 hours. A patch was applied, and the test area was observed 30 minutes, 24 hours, and 120 hours after the patch was removed. Skin reactions were classified according to the level of irritation as follows according to the evaluation criteria of the International Contact Dermatitis Research Group (ICDRG). -: No irritant, ±: Minor irritant (difficult or barely perceptible erythema), +: Mild irritant (well-defined but mild erythema, edema and papules), ++: Moderate irritant (marked erythema, papules and vesicles) ), +++: Indicated as strong irritation (severe erythema, blisters, crust formation), and the results are shown in Table 7 below.

After 30 minutes After 24 hours After 120 hours Manufacturing Example 2-10 - - - Manufacturing Example 2-11 - - - Manufacturing Example 2-12 - - - Manufacturing Example 2-16 - - -

As can be seen in Table 7 above, the ink composition for scalp coloring of the present invention was determined to be non-irritating as a result of the skin irritation test.

In addition, as a result of repeating the treatment three times using the composition of Preparation Example 2-16, it was confirmed that there was no skin irritation and excellent coloring power and coloring persistence were confirmed (see Figure 3).

As seen above, specific embodiments of the present invention have been described in detail, but those skilled in the art who understand the spirit of the present invention may add, change, delete, etc. other components within the scope of the same spirit, thereby creating other regressive inventions. However, other embodiments that are included within the scope of the present invention can be easily proposed. Therefore, the embodiments described above should be understood in all respects as illustrative and not restrictive. The scope of the present invention is indicated by the scope of the claims described below rather than the detailed description above, and all changes or modified forms derived from the meaning and scope of the claims and their equivalent concepts are included in the scope of the present invention. It should be interpreted as

Claims (1)

Step 1 of preparing each extract by extracting black ginger powder, green tea leaf powder, and Ecklonia cava powder using isopropyl alcohol as a solvent, filtering, concentrating, and then freeze-drying;
Step 2 of preparing a natural complex extract by mixing the black ginger extract, green tea leaf extract, and Ecklonia cava extract prepared in Step 1 above at a weight ratio of 2:1:1;
In the method of producing ink for scalp coloring by mixing the natural mixed extract mixed in step 2 with 3% by weight, 7% by weight isopropyl alcohol, 15% by weight carbon black, and 75% by weight distilled water for injection,
The ink for scalp coloring prepared by the above method has excellent coloring power and coloring persistence, does not cause skin irritation, has an effect on skin inflammation, and has an activity to inhibit nitric oxide (NO) production. How to manufacture.