KR20210041251A - Cosmetic composition for scalp comprising Alpinia galanga(L) extract and functional plant extracts - Google Patents

Abstract

The present invention relates to a scalp cosmetic composition comprising, as active ingredients, herbal medicinal extracts such as black ginger extracts and Chinese matrimony vines and various functional plant extracts including herbal extracts, thereby being usable for purposes such as prevention of scalp oxidation, improvement of scalp, prevention of a hair loss, and improvement of inflammation thanks to excellent antioxidant and anti-inflammatory activities thereof. The scalp cosmetic composition according to the present invention comprises 1 to 20 wt% of black ginger extracts with respect to the total weight of the cosmetic composition. Thus, the scalp cosmetic composition has excellent antioxidant and anti-inflammatory activities and can help exfoliate dead skin cells and alleviate sebum. The scalp cosmetic composition does not cause skin irritation, has excellent patch stability, and exhibits excellent formulation stability without deterioration even when stored for a long time. Also, the scalp cosmetic composition can easily improve scalp health and inhibit the occurrence of a hair loss. Thus, the scalp cosmetic composition can be used in formulations such as scaling serum or shampoos.

Description

Cosmetic composition for scalp comprising Alpinia galanga(L) extract and functional plant extracts}

The present invention contains various functional plant extracts including herbal extracts and herbal extracts such as black ginger extract and goji berry as an active ingredient to prevent scalp oxidation and improve scalp, prevent hair loss, and improve inflammation. It relates to a cosmetic composition for scalp that can be used for such purposes.

In recent years, as interest in appearance increases, the use of dyeing, perm, and hair styling products, which have become more frequent than in the past, has increased, leading to an increase in hair damage and hair loss patients, and accordingly, the market size related to hair growth and scalp is also rapidly growing.

The reason why interest in the market related to hair growth and scalp is increasing is that hair loss and baldness are recognized as negatively affecting the quality of life in the modern society where social functions are more important than physiological functions of hair, and there are various ways to improve them. This research is being developed.

Conventionally, as a treatment method for hair loss, minoxidil as a transdermal coating agent and Propecia as an oral dosage form have been used as the only drugs approved by the FDA. Active research is underway to develop alternatives.

On the other hand, black ginger (Alpinia galanga(L)) is a plant that is mainly distributed in Southeast Asia, which is called large Goryanggang, Alpinia galanga, or galangal, and is used as a medicinal or spice, and has excellent antibacterial and anti-inflammatory effects. Rather, it is known to exhibit various physiologically active functions, and various studies to utilize them are actively being conducted.

For example, Korean Patent Publication No. 10-2019-0050048 discloses a composition for controlling nematodes that contains Alpinia galanga extract, cinnamon extract, and neem seed extract as active ingredients to control nematodes in an environment-friendly manner. There is a bar.

As another example, Korean Patent Laid-Open Publication No. 10-2013-0013825 discloses a cosmetic composition for anti-sensitizing skin comprising an acetoxychavicol acetate compound extracted from bulk steel.

However, in the past, studies on the efficacy of improving scalp health and improving keratin and preventing hair loss of black ginger have not been disclosed.

Korean Patent Registration No. 10-2019-0050048 (Publication date: 2019.05.10.) Korean Patent Publication No. 10-2013-0013825 (Publication date: 2013.02.06.)

The inventors of the present invention confirmed that the black ginger extract has excellent antioxidant activity and anti-inflammatory effect, and can be used for purposes such as improving scalp health, preventing hair loss, and improving dead skin cells. When used, it was confirmed that it was possible to manufacture a cosmetic product for scalp with excellent functionality, no skin toxicity, and excellent stability.

Accordingly, the present invention is to provide a technical content for a cosmetic composition for scalp comprising herbal extracts and herbal extracts such as black ginger extract, wolfberry, jujube and the like.

The technical problems to be solved by the present invention are not limited to the technical problems mentioned above, and other technical problems that are not mentioned are clearly understood by those of ordinary skill in the technical field to which the present invention belongs from the following description. It will be possible.

In order to achieve the above-described technical problem, the present invention provides a cosmetic composition for scalp comprising 1 to 20% by weight of black ginger extract based on the total weight of the cosmetic composition.

In addition, the black ginger extract is prepared with an extraction solvent containing at least one selected from the group consisting of alcohol, water, ethyl acetate, ethylene glycol, propylene glycol, butylene glycol, hexane and glycerin having 1 to 4 carbon atoms. Can be used.

According to a preferred embodiment of the present invention, the black ginger extract is a mixture obtained by mixing black ginger raw materials and 50% ethanol in a weight ratio of 1:10, respectively, by heating at a temperature of 50 to 80° C. for 1 to 12 hours, It may be dried and prepared by concentrating 2 to 20 times.

In addition, the cosmetic composition according to the present invention may further include an herbal raw material extract including Goji berry extract, jujube extract, or a mixture thereof, Matricaria Flower extract, Lavender Angustifolia extract, Herbal extracts such as Basil Flower/Leaf Extract, Althaea officinalis Extract, Houttuynia Cordata Extract, and Fennel extract, or a mixture thereof may be further included.

According to a preferred embodiment of the present invention, the cosmetic composition is 10% by weight of black ginger extract, 1% by weight of jujube extract, 1% by weight of Goji berry extract, 60.5% by weight of purified water, 1% by weight of Sodium Lauryl Sulfate. , Ammonium Lauryl Sulfate 1% by weight, Polyquaternium-7 1% by weight, Polyquaternium-10 1% by weight, Lauramide DEA 1% by weight, sh-Polypeptide-16 1% by weight, sh-Polypeptide-4 1% by weight, sh-Polypeptide-22 1% by weight, Tocopheryl Acetate 0.5% by weight, methylchloroisothiazolinone (5 -Chloro-2-methyl-4-isothiazolin-3-one) 0.5% by weight, Matricaria extract 10% by weight, Lavender extract 1% by weight, Basil extract 1% by weight, Marshmallow extract 1% by weight, Salicylic acid 1% by weight %, menthol 1% by weight, apple water 1% by weight, yam extract 1% by weight, fennel extract 1% by weight, it may be formulated as a scaling serum containing 0.5% by weight of fragrance.

In addition, according to a preferred embodiment of the present invention, the cosmetic composition is 5% by weight of black ginger extract, 30% by weight of purified water, 15% by weight of disodium laureth sulfosuccinate, and lauryl glucoside (Lauryl Glucoside) 10% by weight, Decyl glucoside 10% by weight, Cocamido Propyl betaine 5% by weight, TEA-Cocoyl Glutamate 5% by weight, lauramide DIA (Lauramide DEA) 4.5% by weight, Polyquaternium-7 3% by weight, Polyquaternium-10 0.5% by weight, Glycerin 3% by weight, Propylene Glycol 1% by weight , Glycolic acid 0.5% by weight, D-Panthenol 0.5% by weight, Caprylyl Glycol 0.5% by weight, Ethylhexylglycerin 0.5% by weight, lavender extract 1% by weight, rosemary extract 1% by weight, oregano flower/leaf/stem extract 1% by weight, thyme extract 1% by weight, menthol 0.5% by weight, salicylic acid 0.5% by weight, disodium EDTA 0.5% by weight %, may be formulated as a shampoo formulation comprising 0.5% by weight of perfume.

The cosmetic composition for the scalp comprising the extract of herbal ingredients such as black ginger extract and goji japonica according to the present invention includes black ginger extract extracted from natural black ginger, oriental herbal extract extracted from goji japonica, jujube, and herbal extracts, including antioxidant and anti-inflammatory It has excellent activity and can help to remove dead skin cells and improve sebum.

The scalp cosmetic composition as described above does not have skin irritation, has excellent patch stability, and has excellent formulation stability, so that it does not deteriorate even when stored for a long time, so that it can easily improve scalp health and suppress the occurrence of hair loss. It may be formulated and used as a shampoo or the like.

1 is an ABTS and DPPH assay analysis result for confirming _{the reactive oxygen inhibition half-sensitivity (IC 50} ) of black ginger extract prepared by the method according to Examples and Comparative Examples.
2 to 4 are certified test reports of prototype 1 (scaling serum) prepared by the method according to Formulation Example 1.
5 to 7 are certified test reports of prototype 2 (shampoo) prepared by the method according to Formulation Example 2.

Hereinafter, embodiments of the present invention will be described in detail with reference to the accompanying drawings so that those of ordinary skill in the art may easily implement the present invention. However, since the description of the present invention is merely an embodiment for structural or functional description, the scope of the present invention should not be construed as being limited by the embodiments described in the text. That is, since the embodiments can be variously changed and have various forms, the scope of the present invention should be understood to include equivalents capable of realizing the technical idea. In addition, since the object or effect presented in the present invention does not mean that a specific embodiment should include all or only such effects, the scope of the present invention should not be understood as being limited thereto.

The meaning of the terms described in the present invention should be understood as follows.

Terms such as "first" and "second" are used to distinguish one component from other components, and the scope of rights is not limited by these terms. For example, a first component may be referred to as a second component, and similarly, a second component may be referred to as a first component. When a component is referred to as being "connected" to another component, it should be understood that although it may be directly connected to the other component, another component may exist in the middle. On the other hand, when a component is referred to as being "directly connected" to another component, it should be understood that there is no other component in the middle. On the other hand, other expressions describing the relationship between components, that is, "between" and "just between" or "neighboring to" and "directly neighboring to" should be interpreted as well.

Singular expressions are to be understood as including plural expressions unless the context clearly indicates otherwise, and terms such as "comprises" or "have" refer to the specified features, numbers, steps, actions, components, parts, or these. It is to be understood that it is intended to designate that a combination exists and does not preclude the presence or addition of one or more other features, numbers, steps, actions, components, parts, or combinations thereof.

All terms used herein have the same meaning as commonly understood by one of ordinary skill in the field to which the present invention belongs, unless otherwise defined. Terms defined in commonly used dictionaries should be interpreted as having meanings in the context of related technologies, and cannot be interpreted as having an ideal or excessively formal meaning unless explicitly defined in the present invention.

Hereinafter, the present invention will be described in detail.

The cosmetic composition for scalp according to the present invention contains black ginger extract as an active ingredient, and has excellent antioxidant and anti-inflammatory activity, so it can be used to prevent scalp oxidation, improve scalp, prevent hair loss, and improve inflammation.

In the present specification, the black ginger extract refers to an extract prepared using the root of a plant called black ginger, koryanggang, mass river, Alpinia galanga, and Alpinia galanga (L). Since it is also called galanga, it is indicated as'galanga(L)', and in the following, it is collectively referred to as black ginger.

Specifically, the black ginger extract may be prepared using various extraction methods commonly used for the manufacture of cosmetics, and as an example, one prepared by a solvent extraction method may be used, and alcohol, water, and water having 1 to 4 carbon atoms may be used. Representative examples include extraction solvents such as ethyl acetate, ethylene glycol, propylene glycol, butylene glycol, glycerin, or mixtures thereof.

Preferably, the black ginger extract is a mixture of black ginger raw materials and 50% ethanol in a weight ratio of 1:10, respectively, heated at a temperature of 50 to 80° C. for 1 to 12 hours, and then dried and concentrated by 2 to 20 times. It is possible to use what was prepared.

Alternatively, the black ginger extract may be prepared through a supercritical extraction process in which ethanol is used as an extraction solvent, and liquid carbon dioxide is supplied to heat and pressurize, and the black ginger raw material is raw black ginger, dried black ginger, and dried black ginger. Slices, black ginger powder, or a mixture thereof may be used, but are not limited thereto.

The black ginger extract as described above may be included in a ratio of 1 to 20% by weight based on the total weight of the cosmetic composition for scalp, and when the content of the black ginger extract is less than 1% by weight, antioxidant and anti-inflammatory activity and keratin improvement effect There is a minor problem with the back, and if it exceeds 20% by weight, a problem of causing skin irritation may occur.

The cosmetic composition for the scalp according to the present invention may be configured to additionally include herbal extracts such as wolfberry extract, jujube extract, etc., Matricaria extract, Lavender Angustifolia extract, and Basil extract. Extract), marshmallow extract (Althaea officinalis Extract), Yak sorghum extract (Houttuynia Cordata Extract), and fennel extract (Fennel extract). can do.

Herbal herbal extracts and herbal extracts are hot water extraction, alcohol extraction, supercritical fluid extraction, reflux, hot continuous extraction, and microwave extraction, which are commonly used for cosmetics manufacturing. extraction), sonication extraction, and phytonic extraction may be exemplified, and these may be performed alone or mixed to prepare an extract, and may be repeated several times to prepare an extract.

Preferably, the herbal extracts and herbal extracts as described above may be prepared using a variety of extraction methods, for example, those prepared by a solvent extraction method may be used, and alcohol, water, and ethyl having 1 to 4 carbon atoms may be used. Representative examples include an extraction solvent such as acetate, ethylene glycol, propylene glycol, butylene glycol, glycerin, or a mixture thereof.

If you look closely at the herbal raw material extract, goji japonica extract contains ingredients extracted from goji japonica fruit, and when used in combination with black ginger extract, it further enhances the antioxidant effect of the cosmetic composition, and detoxifies, prevents aging, absorbs ultraviolet rays, and converges pores. And the like.

Jujube extract is an extract extracted from jujube fruits, leaves, etc., and acts as a scalp moisturizer and scalp conditioning agent, and together with black ginger extract, it can prevent hair loss by preventing scalp aging.

Goji berry extract and jujube extract may be mixed in a ratio of 0.1 to 10% by weight based on the total weight of the cosmetic composition, respectively, and if the content is less than 0.1% by weight, a slight problem may occur in improving the scalp, and exceeding 10% by weight. In this case, it is difficult to expect additional improvement effects.

In addition, if you look closely at the herbal extract, Matricaria extract, also called chamomile extract, is extracted using Matricaria flowers, stems, and leaves, and contains a large amount of various flavone derivatives and coumarins. It supplies, and acts as an anti-bubble agent, a viscosity reducing agent, an emulsion stabilizer, and a solvent.

Lavender extract promotes cell regeneration, imparts anti-inflammatory and antibacterial effects, reduces skin irritation by interfering with skin conditioning agents and foreign substances harmful to the skin, and imparts skin soothing effects.

Basil Extract is manufactured using basil flowers, leaves, etc., and provides antioxidant and anti-inflammatory effects, gives fragrance, and acts as a skin conditioning agent and antioxidant.

Marshmallow extract is manufactured using the leaves and roots of marshmallow, gives skin soothing effect and anti-inflammatory effect, and Yakmo wheat extract, also called Eoseongcho extract, soothes the scalp, removes sebum, gives hair growth promoting effect, and fennel extract Relieves inflammation and itching of the scalp, tightens, promotes blood circulation, and gives scalp conditioning effects.

In addition, oregano extract is prepared using oregano flowers, leaves, stems, or a mixture thereof, and removes harmful substances from the scalp, improves immunity, and serves as a scalp conditioning agent.

Rosemary extract is manufactured using rosemary leaves, which activates the flow of capillaries, allows nutrients to be properly supplied to the hair follicles, and provides nutrients to the hair roots to grow strong hair, and plays a role as a natural preservative and skin conditioning agent. do.

Thyme extract is manufactured using thyme leaves, and it provides moisturizing, wound healing, and UV protection functions, promotes scalp blood circulation, gives antibacterial properties, and acts as a scalp conditioning agent.

It may be configured to include in a ratio of 0.01 to 20 parts by weight relative to the total weight of the cosmetic composition for scalp as described above, and the content may be adjusted according to the purpose of use of each composition, but is not limited thereto.

In addition, the cosmetic composition according to the present invention may be configured to further include various additives commonly used in the manufacture of cosmetics, if necessary.

Additives include surfactants, carriers, moisturizers, wetting agents, emollients, ultraviolet absorbers, chelating agents, viscosity modifiers, organic powders, preservatives, disinfectants, antioxidants, pH adjusters, alcohols, pigments, pigments, fragrances, blood circulation accelerators , Water (purified water), etc. are representative examples.

In particular, when the composition is used as a cosmetic composition, the cosmetic composition may be prepared in any formulation conventionally prepared in the art. For example, the cosmetic composition may be formulated as a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation, and spray. I can. More specifically, it may be formulated in the form of a flexible lotion, a nutritional lotion, a nutritional cream, a massage cream, an essence, a pack, an eye cream, a cleansing cream, a cleansing foam, a cleansing water, a pack, a spray, or a powder, and is limited thereto. It is not.

The surfactant may be a fatty acid-based surfactant, a cationic surfactant, or an anionic surfactant, and the fatty acid-based surfactant hydrates fatty acids including lauryl acid, myristylic acid, palmitylic acid, and stearylic acid. What is prepared by saponification reaction with potassium can be used, and anionic surfactants such as phosphoric acid ester, isocyanate, sulfonic acid, and taurate, or cationic surfactants such as betaine, glycinate, and alkylamido alkylamine can be used. You can also use it.

In addition, glycerin, dipropylene glycol, propylene glycol, 1,3-butylene glycol, polyethylene glycol, and the like may be mixed and used.

Purified water can be used as a solvent, and various components can be mixed.

According to a preferred embodiment of the present invention, the cosmetic composition is a scaling serum used as a method of washing after applying an appropriate amount to dry scalp and massaging the scalp and hair, and after 3 to 5 minutes have elapsed after application. It can be formulated.

Preferably, the cosmetic composition may be prepared by mixing 10% by weight of black ginger, 55 to 65% by weight of purified water, 3 to 13% by weight of surfactant, 3 to 10% by weight of herbal extract, and 2 to 29% by weight of additives. .

More preferably, the serum is black ginger extract 10% by weight, jujube extract 1% by weight, goji berry extract 1% by weight, purified water 60.5% by weight, sodium lauryl sulfate (Sodium Lauryl Sulfate) 1% by weight, ammonium lauryl sulfate ( Ammonium Lauryl Sulfate) 1% by weight, Polyquaternium-7 1% by weight, Polyquaternium-10 1% by weight, Lauramide DEA 1% by weight, sh- Polypeptide-16 1% by weight, sh-Polypeptide-4 1% by weight, sh-Polypeptide-22 1% by weight, Tocopheryl Acetate 0.5% by weight, methylchloroisothiazolinone (5-Chloro-2-methyl -4-isothiazolin-3-one) 0.5% by weight, Matricaria extract 10% by weight, Lavender extract 1% by weight, Basil flower/leaf extract 1% by weight, Marshmallow leaves/root extract 1% by weight, Salicylic acid 1% by weight %, menthol 1% by weight, apple water 1% by weight, yam extract 1% by weight, fennel extract 1% by weight, and flavoring 0.5% by weight can be prepared by mixing.

In addition, according to a preferred embodiment of the present invention, the cosmetic composition is applied after sufficiently moistening the scalp with water, and after the application, the hair and the scalp are rubbed by hand to sufficiently generate bubbles, and after applying 1 to 2 It can be formulated as a shampoo used as a washing method after minutes have elapsed.

Preferably, the cosmetic composition may be prepared by mixing 5% by weight of black ginger, 25 to 35% by weight of purified water, 35 to 50% by weight of surfactant, 1 to 9% by weight of herbal extract, and 1 to 34% by weight of additives. .

More preferably, the shampoo is black ginger extract 5% by weight, purified water 30% by weight, disodium laureth sulfosuccinate 15% by weight, lauryl glucoside 10% by weight, decyl glucoside (Decyl glucoside) 10% by weight, Cocamido Propyl betaine 5% by weight, TEA-Cocoyl Glutamate 5% by weight, lauramide DEA 4.5% by weight, Polyquaternium-7 3% by weight, Polyquaternium-10 0.5% by weight, Glycerin 3% by weight, Propylene Glycol 1% by weight, glycolic acid ) 0.5% by weight, D-Panthenol 0.5% by weight, Caprylyl Glycol 0.5% by weight, Ethylhexylglycerin 0.5% by weight, Lavender extract 1% by weight, Rosemary extract 1% by weight %, oregano flower/leaf/stem extract 1% by weight, thyme extract 1% by weight, menthol 0.5% by weight, salicylic acid 0.5% by weight, disodium EDTA 0.5% by weight, fragrance 0.5% by weight are mixed It can be manufactured by doing.

Looking in detail about the additives added to the scaling serum and shampoo, salicylic acid balances oil and moisture, removes dead skin cells and sebum, and inhibits dandruff bacteria, and menthol is a denaturant, sweetener, fragrance, and a refreshing feel to the skin. It has the effect of giving, soothing oily skin, helping to protect the skin, and relieving itchiness, and apple water plays a role of exfoliating and conditioning the skin.

In addition, sodium lauryl sulfate is a surfactant, ammonium lauryl sulfate is a synthetic surfactant, and each serves to impart detergency and emulsification, and lauramide DIA promotes foaming and increases viscosity as a water-soluble thickener. It plays a role of giving.

Polyquaternium-7 prevents the generation of static electricity, forms a film, and fixes the hair, Polyquaternium-10 acts as a hair conditioning function, and sh-Polypeptide-16 has a skin protection function, and sh -Polypeptide-4 provides antioxidant and hair and skin conditioning effects, sh-Polypeptide-22 provides skin conditioning, tocopheryl acetate provides skin protection and antioxidant functions, and methylchloroisothiazolinone It improves the sterilization and preservability, and the fragrance plays a role of imparting a fragrance.

In addition, propylene glycol acts as a moisturizing agent, skin conditioning agent, solvent, and viscosity reducing agent, Glycolic Acid removes dead skin cells and acts as a pH adjuster, and D-panthenol provides a moisturizing effect. And, Caprylyl Glycol and ethylhexyl glycerin serve as skin softening agents and hair conditioning agents.

Purified water can be used as a solvent, and various components can be mixed.

On the other hand, with regard to environmental variables of alopecia, in studies on the level of antioxidants and lipid peroxidation in the blood of alopecia patients, the activity of antioxidants that protects against oxidative damage caused by reactive oxygen species such as free radicals and peroxides is significantly lower. On the other hand, it is reported that the indexes for assessing lipid peroxidation and oxidative stress are reported to be significantly high, so oxidative stress is highly correlated with the occurrence of deguarantee, and antioxidants prevent oxidative damage to scalp cells and cell aging, resulting in hair loss It is known that it can improve.

An approach to treating hair loss by using an antioxidant mechanism that suppresses oxidative damage to cells due to aging and environmental influences or by regulating immune factors that affect the physiology of scalp cells is currently being proposed as a treatment method for hair loss. There is an advantage in avoiding the side effects of the methods.

The cosmetic composition according to the present invention as described above has excellent antioxidant and anti-inflammatory activity, including black ginger extract extracted from natural black ginger, herbal extracts and herbal extracts extracted from wolfberry, jujube, etc., and helps to remove dead skin cells and improve sebum. Not only can it give skin irritation, it is excellent in patch stability, and it has excellent formulation stability, so it does not deteriorate even when stored for a long time. Therefore, the cosmetic composition according to the present invention can be used for introduction into the scalp such as scalp exfoliation, scalp sebum improvement, scalp oxidation prevention, scalp health improvement, scalp inflammation improvement, scalp hair loss prevention, and the like.

Hereinafter, the present invention will be described in more detail by way of examples.

The examples presented are only specific examples of the present invention, and are not intended to limit the scope of the present invention.

<Example 1> Preparation of black ginger EtOH extract 1

(1) Preparation of black ginger extract

350 g of black ginger raw material (Alpinia galanga(L) powder) and 35 L of extraction solvent were each supplied to an extraction device (TIC-G20 EXTRATOR), and 1% black ginger extract was prepared by heating at a temperature of 60° C. for 4 hours. . The extraction solvent used to prepare the black ginger extract was hydrous ethanol with 50% alcohol content, and was prepared by mixing 18.4 L of 95% ethanol and 16.6 L of water.

(2) Concentrated black ginger extract

35 L of the prepared black ginger extract was supplied to a large-capacity rotary concentrator, heated to prepare a 4 L concentrate, and 8.75% black ginger ethanol extract (black ginger EtOH extract 1) was prepared.

<Example 2> Preparation of black ginger EtOH extract 2

(1) Preparation of black ginger extract

35 g of black ginger raw material powder and 40 mL of 50% hydrated ethanol were supplied to the vacuum extraction device, respectively, and a supercritical extraction process was performed for 120 minutes while adjusting the temperature and pressure to 40 ℃ and 150 bar. Ginger ethanol extract 2 (black ginger EtOH extract 2) was prepared.

The supercritical extraction process was performed using a vacuum extraction device connected to a liquid carbon dioxide storage tank and a solvent supply tank supplying the extraction solvent and a pipe, and the vacuum extraction device is equipped with a vacuum pump to maintain the vacuum state, the temperature of the extraction tank. It was used that is provided with a temperature control member to control. Liquid carbon dioxide at 25 to 30° C. was supplied to the extraction tank, and the extraction process was performed under conditions of maintaining a vacuum, and the flow rate was adjusted to 50 mL/min.

<Comparative Example 1> Preparation of black ginger DW extract

Black ginger extract was prepared in the same manner as in Example 1, except for mixing 350 g of black ginger raw material and 35 L of distilled water, and then concentrated to prepare 8.75% black ginger hot water extract (black ginger DW extract).

<Comparative Example 2> Preparation of black ginger MeOH extract

Black ginger extract was prepared in the same manner as in Example 1, except for mixing 350 g of black ginger raw material and 35 L of 80% methanol, and then concentrated to prepare 8.75% black ginger methanol extract (black ginger MeOH extract).

<Comparative Example 3> Preparation of black ginger Hex extract

Black ginger extract was prepared in the same manner as in Example 1, except for mixing 350 g of black ginger raw material and 35 L of hexane, and then concentrated to prepare 8.75% black ginger hexane extract (black ginger Hex extract).

<Comparative Example 4> Preparation of EA extract of black ginger

Black ginger extract was prepared in the same manner as in Example 1, except for mixing 350 g of black ginger raw material and 35 L of ethyl acetic acid, and then concentrated to prepare 8.75% black ginger ethylacetic acid extract (black ginger EA extract).

<Formulation Example 1> Preparation of serum containing black ginger EtOH extract (prototype 1)

Purified water, sodium lauryl sulfate, ammonium lauryl sulfate, polyquaternium-7, polyquaternium-10, lauramide DEA), sh-Polypeptide-16, sh-Polypeptide-4, sh-Polypeptide-22, Tocopheryl Acetate, 5-Chloro-2-methyl-4-isothiazolin-3- one), Matricaria extract, lavender extract, basil flower/leaf extract, marshmallow leaf/root extract, salicylic acid, menthol, apple water, yam extract, fennel extract, fragrance, black ginger EtOH extract 1, jujube extract, goji berry Using the extract, a serum was prepared in the composition as shown in Table 1 below.

<Formulation Example 2> Preparation of shampoo containing black ginger EtOH extract (prototype 2)

Purified water, Disodium Laureth sulfosuccinate, Lauryl Glucoside, Decyl glucoside, Cocamido Propyl betaine, TEA-Cocoyl Glutamate (TEA) -Cocoyl Glutamate), Lauramide DEA, Polyquaternium-7, Polyquaternium-10, Glycerin, Propylene Glycol, Glycolic Acid ( glycolic acid), D-Panthenol, Caprylyl Glycol, Ethylhexylglycerin, lavender extract, rosemary extract, oregano flower/leaf/stem extract, thyme extract, menthol, saly A shampoo was prepared in the composition as shown in Table 2 below using silicic acid, disodium EDTA, flavoring, and black ginger EtOH extract.

In the preparation of a prototype product including a serum and a shampoo, it is preferable to mix the raw materials dispersed in the water phase using a mixer, and sufficiently increase the viscosity, and then add and mix black ginger extract.

Specifically, each component belonging to category A is mixed and heated to 80°C to uniformly mix to prepare a mixture A, and components belonging to category B are uniformly mixed and dispersed to prepare a mixture B, and then mixture A and Mixing the mixture B, uniformly mixing each component belonging to category C to prepare a mixture C, mixing with the mixture of A and B, and finally mixing the components belonging to the category D, each A phase component mixture, B phase component mixture, and C phase component mixture each prepared using purified water are prepared, and then sequentially mixed to prepare a product formulation having a uniform viscosity.

<Formulation Example 3> Preparation of serum containing black ginger EtOH extract 2 (prototype 3)

A serum was prepared in the same manner as in Formulation Example 1, except that black ginger EtOH extract 2 was used.

<Formulation Example 4> Preparation of shampoo containing black ginger EtOH extract 2 (prototype 4)

A shampoo was prepared in the same manner as in Formulation Example 2, except that black ginger EtOH extract 2 was used.

<Experimental Example 2> Analysis of active ingredient content

(1) Total flavonoid content analysis

65 µl of black ginger extract sample was mixed with 20 µl of 5% sodium nitrite (NaNO ₂ ) and reacted for 5 minutes, and _{39 µl of 10% aluminum chloride (AlCl 3} ·6H ₂ 0) was supplied to the reaction product and reacted for 6 minutes. Then, 130 µl of 1M sodium hydroxide (NaOH) was supplied and reacted for 11 minutes, and then absorbance was measured at 510 nm with an ELISA Reader, and the results are shown in Table 3 below.

(2) Analysis of total polyphenol content

200 µl of the black ginger extract sample _{was mixed with 4 mL of 2% sodium carbonate (Na 2} CO ₃ ) and reacted for 3 minutes, and 200 µl of 50% folin solution (Folin-Ciocalteu's Phenol reagent) was additionally added and reacted for 30 minutes. Next, absorbance was measured at 750 nm with a spectrophotometer, and the results are shown in Table 4 below.

<Experimental Example 2> Analysis of efficacy of black ginger extract

1. Antioxidant activity assay

(1) ABTS assay

7 mM ABTS solution and 2.45 mM potassium persulfate were each mixed at a weight ratio of 1:1, and then reacted in the dark for 24 hours to prepare an ABTS reagent. Black ginger extract samples and positive control (Positive Control) were dispensed 10 μl into a 96 well plate (10 μl of distilled water was dispensed into the control). The ABTS reagent was diluted 20 times, and then the diluted ABTS reagent was dispensed 190 µl into each well (190 µl of distilled water was dispensed to zero). After reacting at room temperature for 30 minutes, each reaction was measured for absorbance at 700 nm with an ELISA Reader, and the analysis results are shown in Table 5 below.

(2) DPPH assay

The black ginger extract sample and the positive control were dispensed with 10 μl each into a 96 well plate (10 μl of ethanol was dispensed into the control). 190 μl of 0.15 mM DPPH Solution was dispensed per well into each well, and reacted at room temperature for 30 minutes, and then the absorbance of each reaction was measured at 517 nm with an ELISA Reader, and the analysis results are shown in Table 6 below.

As shown in Tables 5 and 6, the ABTS assay and DPPH assay analysis of measuring antioxidant activity by checking the free radical scavenging ability, it was confirmed that the black ginger extract prepared by each method all exhibited antioxidant activity. , In particular, it was confirmed that the antioxidant activity of the ethanol extract of black ginger was the most excellent.

(3) Reactive oxygen inhibition half-sensitivity of black ginger extract (IC ₅₀ ) analysis

Reactive oxygen inhibition half-sensitivity of the black ginger extract was analyzed, and the results are shown in Table 7 and FIG. 1 below.

As shown in Table 7 and Figure 1, as a result of analyzing the _{reactive oxygen inhibitory half-sensitivity (IC 50 ), the IC 50} value of the ethanol extract was 125.5 ± 9.7 µg/mL for the ABTS assay, and 229.1 ± 2.4 µg for the DPPH assay. /mL, and as a result of analyzing the total flavonoid and total polyphenol content, it was confirmed that the ethanol extract of black ginger, which has excellent antioxidant activity, had the highest total flavonoid and total polyphenol content compared to other extracts. In particular, it was confirmed that the ABTS and DPPH scavenging activity was the most excellent in the case of EtOH extract 2 of black ginger.

Through the above facts, it was confirmed that the prepared black ginger extract exhibited excellent free radical scavenging activity, thereby reducing oxidative stress on the scalp, improving scalp health, and preventing hair loss.

<Experimental Example 3> Analysis of anti-inflammatory activity of black ginger extract

(1) MTT assay

The cells were dispensed into a 96 well plate at a ^{concentration of 5 × 10 5} cells/well by 190 μl, and cultured for 24 hours in a _{37°C, 5% CO 2 incubator.} After 4 hours of dispensing the cells, 5 µl of the black ginger extract sample was treated for each concentration, and 5 µL of LPS was treated at the time of 1 hour, and in an _{incubator at 37°C, 5% CO 2} for 24 hours. During incubation.

To perform the MTT assay, 100 µl of each cell culture solution was taken, and 10 µl of each MTT reagent was treated in a 96-well plate in which the remaining 100 µl of cell culture solution remained, and then 37 ℃, 5% CO ₂ incubator Cell viability was measured by incubating for 2 hours at.

The cell viability was measured by measuring absorbance at a wavelength of 450 nm, and when the cell viability was reduced to less than 90% of the sample-free group, it was determined to be potentially cytotoxic. It was administered in the configuration as shown in Table 8, and the cell viability was calculated according to the following formula, and the results are shown in Table 9 below.

[Equation 1]

(2) NO assay

In addition, the cells ^{were dispensed into a 96 well plate at a concentration of 5 × 10 5} cells/well 190 μl each, and cultured for 24 hours in a _{37° C., 5% CO 2 incubator.} After 4 hours of dispensing the cells, 5 µl of the black ginger extract sample was treated for each concentration, and 5 µL of LPS was treated at the time of 1 hour, and in an _{incubator at 37°C, 5% CO 2} for 24 hours. During incubation.

The cell culture solution and the Griess reagent were each mixed at a weight ratio of 1:1, and the absorbance was measured at 550 nm wavelength after 10 minutes, and the black ginger extract was administered in the configuration as shown in Table 8 above, and the amount of NO produced. The NO content in the culture medium was calculated by preparing a standard curve for each concentration with sodium nitrite, and the results are shown in Table 9.

As shown in Table 9, as a result of evaluating the cytotoxicity of the black ginger extract, the black ginger hot water extract did not show cytotoxicity at all concentrations, and the black ginger methanol extract showed cytotoxicity only at the highest concentration of 200 μg/mL. Was confirmed. In addition, black ginger ethanol extract 1, black ginger hexane extract, and black ginger ethyl acetate extract were found to exhibit cytotoxicity at concentrations of 50 µg/mL or more, respectively, and black ginger ethanol extract 2 was cytotoxicity at concentrations of 200 µg/mL or more. It was confirmed to represent.

In addition, as a result of evaluating nitric acid inhibitory activity, when comparing the NO inhibitory activity at the concentration of 25 μg/mL of all extract samples that did not show cytotoxicity, it was found that the NO inhibitory activity effect of black ginger EtOH extracts 1 and 2 was the most excellent. As a result, it was confirmed that the black ginger EtOH extract has the best anti-inflammatory activity and can effectively suppress inflammation.

(3) Analysis of antibacterial activity against acne bacteria

Using the Gram-positive bacterium Propionibacterium acnes as a test strain, a Halo test was performed to evaluate the antibacterial effect of the black ginger extract. To this end, the test strain was inoculated into Reinforced clostridial medium (RCM) broth and cultured at (35 ± 1) ℃ for 3 days, and the cultured bacterial solution was inoculated into a new RCM broth and cultured under the same conditions twice. Thus, the test bacteria were pre-cultured. In all culture processes, sterile liquid paraffin was used to maintain anaerobic conditions.

Inoculate the fungal culture solution evenly on the prepared Reinforced clostridial medium (RCM) agar with a sterilized cotton swab, and supply each black ginger extract and 25 µl of the prototype to the center of the medium, and multiply for 72 hours at an anaerobic condition (35 ± 1) ℃ Next, the antibacterial activity was analyzed by measuring the zone of inhibition around the sample.

Antibacterial activity analysis was evaluated using the following formula 2, and the results are shown in Table 10 below.

[Equation 2]

(However, in Equation 1, W is the width of the lowland (mm), T is the total diameter of the sample and the lowland (mm), and D is the diameter of the sample (mm))

As shown in Table 10, as a result of evaluating the antibacterial activity of the black ginger extract and the prototype against acne bacteria, it was confirmed that the black ginger EtOH extract, the prototype 1, and the prototype 2 had excellent antibacterial activity. It was judged to be excellent in improving scalp health.

<Experimental Example 4> Evaluation of a prototype

(1) Ingredient safety evaluation of prototypes 1 and 2

The safety of prototypes 1 and 2 manufactured according to the Ministry of Food and Drug Safety Notice No. 2019-27 was evaluated, and the results are shown in Table 11 (Prototype 1) and Table 12 (Prototype 2), respectively.

(* However, in Table 9 and Table 10 below, the Method Detection Limit (MDL) is, Pb: 2 µg/g, As: 1 µg/g, Sb: 1 µg/g, Cd: 1 µg/g , Ni: 1 µg/g, Hg: 0.05 µg/g, 1,4-Dioxane: 1 µg/g, Formaldehyde: 4 µg/g, DBP, BBP, DEHP: 5 µg/g, Methanol: 0.0005 (v/ v)%)

As shown in Tables 11 and 12, it was confirmed that both the prototype 1 and the prototype 2 had the results satisfying the standards notified by the Ministry of Food and Drug Safety (distribution cosmetics safety management items), so that safety was sufficiently secured (Fig. Refer to the certified test report of 2 to 7 [Prototype 1 and Prototype 2]).

(2) Evaluation of the formulation safety of the prototype

In order to evaluate the formulation stability of the prepared prototype, pH change measurement, temperature stability, and formulation change were confirmed, and the formulation stability was evaluated for a total of 60 days. The prepared prototypes 1 and 2 were divided into 3 containers, respectively, and stored for 60 days, and it was checked whether there was any change.

First, pH measurement was performed by measuring the pH of the prototypes 1 and 2 stored at 25°C. Before the measurement, the glass electrode was immersed in a basic buffer solution or distilled water for several hours, and the pH meter was connected to a power source and left for 10 minutes or longer before use. The detector was well washed with distilled water and lightly wiped before use.

Next, in the stability test method according to temperature, each prototype was stored for each temperature condition (0°C, 25°C, 45°C) to evaluate the state change according to the change over time.

As a result, it was confirmed that all of the prototypes 1 to 4 had no change in pH, odor, color, and viscosity at each temperature of 0°C, 25°C, and 45°C, and that no precipitate was generated.

<Experimental Example 5> Clinical evaluation of a prototype

Clinical trials of the prototype were conducted to evaluate the safety and harmlessness of the product. Clinical evaluation was performed by requesting the Clinical Support Center for Oriental Medicine Bio Industry at Semyung University.

(1) sebum improvement effect analysis

The evaluation method was performed through the instrument measurement method using Sebumeter (SM815, CK electronics, Germany), and the effectiveness result was the result of measuring the amount of temporary sebum using the Sebumeter, and the statistically significant level of temporary sebum improvement after using the product compared to before using the product. There was an effect (p<0.05).

The clinical evaluation was performed on 20 adult women aged 30 to 45 years. The test product was used on the scalp according to the method of use, and the effect of temporarily improving scalp sebum was evaluated. The evaluation was performed in accordance with the Center's internal guidelines (SOP), and for matters not specified in the Ministry of Food and Drug Safety Notice, reference was made to reference literature.

In order to measure the scalp, which is the test site, the test site was kept clean and dry without any hair products applied in order to make the test conditions the same, and constant temperature and humidity (22±2℃, RH 40~60%) for at least 30 minutes. ) To keep the scalp stable, and then proceed afterwards. Temporary sebum volume was measured at three locations on the crown using a Sebumeter, and the measurement was analyzed with a dedicated program (MPA5, CK electronics, Germany). Sebumeter was performed by measuring the sebum adsorbed on the film after contacting the film located on the cassette head part of the ^{64 mm 2 area for a certain period of time.} Using the principle of increasing transparency when sebum is adsorbed on the film, the film was brought into contact with the skin for 30 seconds at a constant pressure, and then the level of lipids was measured using photometry.

After checking the condition of the subject's scalp before using the product, a device evaluation (Sebumeter) was performed, and after using the product, the test product was used according to the method of use, and skin irritation evaluation and device evaluation were performed. Erythema, edema, scaling, itching, stinging, burning, stiffness, prickling, which are adverse reactions to the skin irritation evaluation test product. The presence or absence of or other adverse reactions was closely observed, and if any adverse skin reactions were found, the grade was marked according to the severity and the test findings were prepared.

According to each method of use, the scalp sebum was analyzed before and after use of the product and then the scalp sebum was analyzed, and the average value was used by repeating measurements three times using a Sebumeter, and the unit was expressed as the amount of sebum per area (㎍/cm ² ). , The smaller the measured value, the lower the amount of sebum per area.

Statistical analysis was performed using a paired t-test method to compare differences before and after product use. All statistical results were considered to be statistically significant when the significant difference of 5% (p<0.05), which is the most used in biological statistical analysis, was considered to be statistically significant, and SPSS 18.0 software was used for statistical analysis.

(2) Analyze the effect of improving keratin

The evaluation method was evaluated visually after taking an image of keratin using Visioscan (VC98, Courage and Khazaka Electronic Co. Germany), and the efficacy result was the result of measuring scalp keratin through visual evaluation after image capture using Visioscan, compared with before use of the product. As a result, it showed a statistically significant temporary scalp keratin improvement effect after using the product (p<0.05).

Temporary scalp keratin measurement Visual evaluation was evaluated by two skilled researchers (BSH, JEM). When there is a significant difference in the evaluation results of the two experts, a high keratin level was selected, and photographing was performed using a Folliscope® 4.0 device in parallel, and the differences before and after the use of the product were compared and examined. For evaluation, a 6-point evaluation method was used, and 1 point was classified as the least and 6 points as the most.

(3) Evaluation of skin patch stability

The evaluation method was evaluated by conducting skin irritation evaluation according to the evaluation criteria presented by the International Society for Contact Dermatitis, and was conducted through the Clinical Support Center for Oriental Medicine Bio Industry at Semyung University, and a 24-hour skin patch (closed patch) method using a Finn chamber. Performed.

The evaluation was performed on 30 adult males and females aged 20 to 55 years old, and each 25 μl of prototype 1 and 2 diluted by 1% with distilled water were dropped into the Finn Chamber and placed on the subject's back for fixation. Was carried out. After 24 hours, the patch was removed, and the test site was marked with a Marking Pen. After 30 minutes, 24 hours, and 48 hours, the test site was observed and the skin reaction was evaluated. The evaluation was performed in accordance with the Center's internal guidelines (SOP), and for matters not specified in the Ministry of Food and Drug Safety Notice, reference was made to reference literature.

The judgment was made 30 minutes and 24 hours after the patch was removed, and the skin reaction was classified according to the criteria of the International Contact Dermatitis Research Group (ICDRG) shown in Table 15 below. The average skin reactivity (Mean score) was calculated according to the calculation formula in Table 2, and the presence or absence of irritation was determined according to the judgment table using the skin patch test results.

As a result of performing the skin patch safety human application test, it was confirmed that the prototypes 1 to 4 belonged to the non-irritating product group according to the skin irritation (Mean score) evaluation. As a result of the skin irritation evaluation, no special skin adverse reactions were found during the test period.

In addition, as a result of visual evaluation after taking an image of keratin using Visioscan (VC98, Courage and Khazaka Electronic Co. Germany), the effect of improving temporary scalp keratin was statistically significant after using the product compared to before using the prototypes 1 to 4. It was confirmed that it was shown (p<0.05).

In addition, as a result of measuring the amount of temporary sebum using a Sebumeter (SM815, CK electronics, Germany), it was confirmed that the temporary sebum improvement effect was exhibited at a statistically significant level after the product was used compared to before the product was used (p<0.05).

Based on the above facts, it was demonstrated that the prototypes manufactured do not have skin irritation and have excellent scalp keratin and sebum improvement effects, so that they can be introduced into the scalp for purposes such as improving scalp health, preventing hair loss, improving inflammation, and preventing scalp oxidation. [Experimental results related to the effect of improving scalp keratin and sebum are the Semyung University Oriental Medicine Bio Industry Clinical Support Center (research management number: IQ-6546-A and IQ-6546-B, report management number: SMC-190730-4146 and SMC-190730-4147).

Detailed description of the preferred embodiments of the present invention disclosed as described above has been provided to enable those skilled in the art to implement and practice the present invention. Although the above has been described with reference to preferred embodiments of the present invention, those skilled in the art will understand that various modifications and changes can be made to the present invention without departing from the scope of the present invention. For example, those skilled in the art may use the configurations described in the above-described embodiments in a manner that combines them with each other. Accordingly, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.

The present invention may be embodied in other specific forms without departing from the spirit and essential features of the present invention. Therefore, the detailed description above should not be construed as restrictive in all respects and should be considered as illustrative. The scope of the present invention should be determined by reasonable interpretation of the appended claims, and all changes within the equivalent scope of the present invention are included in the scope of the present invention. The invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein. In addition, the embodiments may be configured by combining claims that do not have an explicit citation relationship in the claims, or may be included as new claims by amendment after filing.

Claims (7)

A cosmetic composition for scalp comprising 1 to 20% by weight of black ginger extract based on the total weight of the cosmetic composition. The method of claim 1,
The black ginger extract is characterized in that it is prepared with an extraction solvent containing at least one selected from the group consisting of alcohol, water, ethyl acetate, ethylene glycol, propylene glycol, butylene glycol, hexane and glycerin having 1 to 4 carbon atoms. Cosmetic composition for scalp. The method of claim 1,
The black ginger extract,
A scalp, characterized in that a mixture obtained by mixing black ginger raw materials and 50% ethanol in a weight ratio of 1:10, respectively, is heated at a temperature of 50 to 80 °C for 1 to 12 hours, and then dried and concentrated by 2 to 20 times. Cosmetic composition. The method of claim 1,
A cosmetic composition for scalp, characterized in that it further comprises at least one herbal raw material extract selected from the group consisting of wolfberry extract and jujube extract. The method of claim 1,
Group consisting of Matricaria Flower extract, Lavender Angustifolia extract, Basil Extract, Althaea officinalis Extract, Houttuynia Cordata Extract, and Fennel extract Cosmetic composition for scalp, characterized in that it further comprises one or more herbal extracts selected from. The method of claim 1,
Black ginger extract 10% by weight, jujube extract 1% by weight, wolfberry extract 1% by weight, purified water 60.5% by weight, Sodium Lauryl Sulfate 1% by weight, Ammonium Lauryl Sulfate 1% by weight, Polyquaternium-7 1% by weight, Polyquaternium-10 1% by weight, Lauramide DEA 1% by weight, sh-Polypeptide-16 1% by weight, sh -Polypeptide-4 1% by weight, sh-Polypeptide-22 1% by weight, Tocopheryl Acetate 0.5% by weight, methylchloroisothiazolinone (5-Chloro-2-methyl-4-isothiazolin-3-one ) 0.5% by weight, 10% by weight of Matricaria extract, 1% by weight of lavender extract, 1% by weight of basil flower/leaf extract, 1% by weight of marshmallow leaves/root extract, 1% by weight of salicylic acid, 1% by weight of menthol, apple A cosmetic composition for a scalp, characterized in that it is a serum formulation containing 1% by weight of water, 1% by weight of Yakmi extract, 1% by weight of fennel extract, and 0.5% by weight of fragrance. The method of claim 1,
The black ginger extract 5% by weight, purified water 30% by weight, disodium laureth sulfosuccinate 15% by weight, lauryl glucoside 10% by weight, decyl glucoside 10% by weight , Cocamido Propyl betaine 5% by weight, TEA-Cocoyl Glutamate 5% by weight, Lauramide DEA 4.5% by weight, Polyquaternium-7 -7) 3% by weight, polyquaternium-10 0.5% by weight, glycerin 3% by weight, propylene glycol (Propylene Glycol) 1% by weight, glycolic acid 0.5% by weight, D- Panthenol 0.5% by weight, Caprylyl Glycol 0.5% by weight, Ethylhexylglycerin 0.5% by weight, Lavender extract 1% by weight, Rosemary extract 1% by weight, Oregano flower/leaf/ It is characterized in that it is a shampoo formulation containing 1% by weight of stem extract, 1% by weight of thyme extract, 0.5% by weight of menthol, 0.5% by weight of salicylic acid, 0.5% by weight of Disodium EDTA, and 0.5% by weight of fragrance. Cosmetic composition for scalp.

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