KR102665549B1 - Lactobacillus brevis neolacto-nd01 strain and skin moisturizing composition containing the strain as an active ingredient - Google Patents

Abstract

The present invention relates to Lactobacillus brevis NEOLACTO-ND01 strain and a skin moisturizing composition containing the strain as an active ingredient. The Lactobacillus brevis NEOLACTO-ND01 strain (accession number KCTC 15287BP) of the present invention increases the expression of intracellular hyaluronic acid synthase (HAS-3) and has the effect of inhibiting the enzyme activity of hyaluronidase. Therefore, it can be usefully used to improve skin moisturization.

Description

Lactobacillus brevis NEOLACTO-ND01 strain and a skin moisturizing composition comprising the strain as an active ingredient {LACTOBACILLUS BREVIS NEOLACTO-ND01 STRAIN AND SKIN MOISTURIZING COMPOSITION CONTAINING THE STRAIN AS AN ACTIVE INGREDIENT}

The present invention relates to Lactobacillus brevis NEOLACTO-ND01 strain and a skin moisturizing composition containing the strain as an active ingredient.

Skin covers the entire human body and provides a protective barrier against various forms of external irritation and damage. The skin also protects against water loss, referred to as dehydration. Structurally, the skin is a multilayer membrane with a very complex structure and includes the epidermis, the outermost epithelial tissue of the skin. The epidermis is mainly composed of keratinocytes and consists of four layers: stratum corneum, stratum granulosum, stratum spinosum, and stratum basale. The stratum corneum, the outermost layer of the epidermis, is composed of dead cells (keratinocytes). The main function of the stratum corneum is to form a barrier to protect the underlying tissue from infection, dehydration, chemicals, and mechanical stress.

It has long been recognized that skin function is highly dependent on the amount of water available in the stratum corneum. Changes in the water content of the stratum corneum often have a significant impact on the functional properties of the skin. Therefore, it is essential to provide sufficient moisture to the stratum corneum for healthy skin.

Recently, a number of studies have been reported and have focused on supplying water to the skin from external sources or preventing the loss of water from the body to maintain skin moisture content at an appropriate level. As a result, various types of topical moisturizers with moisture-retaining abilities have been developed and are mainly used in cosmetics, skin care, personal hygiene, and pharmaceutical fields.

However, a significant number of people continue to suffer from dry skin symptoms. Additionally, as people age, their skin loses elasticity and its ability to retain moisture decreases. Therefore, as many people experience dry skin as they age, an enhanced formulation has the ability to help moisture penetrate the skin more easily, quickly and deeply so that hydration is achieved more quickly and retained for a longer period of time. and methods are required.

Accordingly, there is still a need for compositions and methods to improve the hydration or moisturizing level of the skin to obtain rapid and sustained effects that can be conveniently and effectively achieved.

Republic of Korea Patent Publication No. 10-2021-0155987

The present invention relates to Lactobacillus brevis NEOLACTO-ND01 strain (accession number: KCTC 15287BP) and a culture of the strain, a concentrate of the culture, a dried product of the culture, an extract of the culture, and 1 selected from the group consisting of dead cells of the strain. The purpose is to provide a skin moisturizing composition containing more than one type of active ingredient.

According to one embodiment of the present invention, Lactobacillus brevis ( Lactobacillus brevis ) NEOLACTO-ND01 strain (accession number KCTC 15287BP) is provided.

The strain is characterized by having the 16S rRNA sequence of SEQ ID NO: 1.

The strain has the effect of increasing the expression of intracellular hyaluronic acid synthase (HAS-3).

The strain has the effect of inhibiting the enzymatic activity of hyaluronidase.

According to another embodiment of the present invention, the Lactobacillus brevis NEOLACTO-ND01 strain (accession number KCTC 15287BP) culture, concentrate of the culture, dried material of the culture, extract of the culture, and dead cells of the strain Provided is a cosmetic composition for skin moisturizing containing as an active ingredient at least one selected from the group consisting of.

The cosmetic composition may be selected from the group consisting of solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation and spray. , but is not limited to this.

According to another embodiment of the present invention, the Lactobacillus brevis NEOLACTO-ND01 strain (accession number KCTC 15287BP) culture, behenyl alcohol, stearic acid, polysorbate 60, sorbitan sesquioleate, and sorbitan stear. A skin moisturizing cream composition comprising late, self-emulsifying glycerin monostearate, squalane, propanediol, glycerin, carboxypolymer, triethanolamine, 1,2 hexanediol, and purified water.

According to another embodiment of the present invention, the Lactobacillus brevis NEOLACTO-ND01 strain (accession number KCTC 15287BP) culture, concentrate of the culture, dried material of the culture, extract of the culture, and dead cells of the strain Provides a health functional food for skin moisturizing containing as an active ingredient one or more types selected from the group consisting of.

The Lactobacillus brevis NEOLACTO-ND01 strain (accession number KCTC 15287BP) of the present invention increases the expression of intracellular hyaluronic acid synthase (HAS-3) and has the effect of inhibiting the enzyme activity of hyaluronidase. Therefore, it can be usefully used to improve skin moisturization.

The present invention relates to the Lactobacillus brevis NEOLACTO-ND01 strain (accession number KCTC 15287BP), which has a skin moisturizing effect, which increases the expression of intracellular hyaluronic acid synthase (HAS-3) and hyaluronic acid synthase. It has the effect of inhibiting the enzymatic activity of ronidase.

The Lactobacillus brevis NEOLACTO-ND01 strain (accession number KCTC 15287BP) contains glucose, yeast extract, dipotassium phosphate, magnesium sulfate, and manganese sulfate ( It can be cultured in a medium containing Manganese Sulfate).

In addition, the present invention relates to a culture of Lactobacillus brevis NEOLACTO-ND01 strain (accession number KCTC 15287BP) having the skin moisturizing effect, a concentrate of the culture, dried product of the culture, extract of the culture, and dead cells of the strain. Provided is a cosmetic composition for skin moisturizing containing as an active ingredient at least one selected from the group consisting of.

The ingredients included in the cosmetic composition of the present invention are active ingredients and include ingredients commonly used in cosmetic compositions in addition to the above-mentioned active ingredients, such as antioxidants, stabilizers, solubilizers, vitamins, conventional auxiliaries such as pigments and fragrances, And it includes a carrier.

The cosmetic composition of the present invention can be prepared in any formulation commonly prepared in the art, for example, solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, surfactant-containing cleansing products. , oil, powder foundation, emulsion foundation, wax foundation, spray, etc., but is not limited thereto. More specifically, it can be manufactured in the form of softening lotion, nourishing lotion, nourishing cream, massage cream, essence, eye cream, cleansing cream, cleansing foam, cleansing water, pack, spray, or powder.

When the formulation of the present invention is a paste, cream or gel, animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as the carrier ingredient. You can.

When the formulation of the present invention is a powder or spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, or polyamide powder can be used as the carrier ingredient. In particular, when the formulation is a spray, chlorofluorohydrocarbon and propane may be used as carrier ingredients. /May contain propellants such as butane or dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, solubilizing agent, or emulsifying agent is used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 , 3-butyl glycol oil, fatty esters of glycerol, fatty acid esters of polyethylene glycol or sorbitan.

When the formulation of the present invention is a suspension, the carrier ingredients include water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester, and polyoxyethylene sorbitan ester, and microcrystals. Cellulose, aluminum metahydroxide, bentonite, agar, or tracant may be used.

When the formulation of the present invention is a surfactant-containing cleansing agent, the carrier ingredients include aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyl taurate, sarcosinate, and fatty acid amide. Ether sulfate, alkylamidobetaine, fatty alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable lanolin derivative, or ethoxylated glycerol fatty acid ester may be used.

When the cosmetic composition is manufactured as a cream, the Lactobacillus brevis NEOLACTO-ND01 strain (accession number KCTC 15287BP) culture, behenyl alcohol, stearic acid, polysorbate 60, sorbitan sesquioleate, sorbitan stear It may contain ester, self-emulsifying glycerin monostearate, squalane, propanediol, glycerin, carboxypolymer, triethanolamine, 1,2 hexanediol, and purified water.

In addition, the present invention relates to a culture of Lactobacillus brevis NEOLACTO-ND01 strain (accession number KCTC 15287BP) having the skin moisturizing effect, a concentrate of the culture, dried product of the culture, extract of the culture, and dead cells of the strain. Provides a health functional food for skin moisturizing containing as an active ingredient one or more types selected from the group consisting of.

The above-mentioned health functional foods refer to foods manufactured and processed in the form of tablets, capsules, powders, granules, liquids, and pills using raw materials or ingredients with functional properties useful to the human body. Here, ‘functionality’ means controlling nutrients for the structure and function of the human body or obtaining useful effects for health purposes, such as physiological effects. The health functional food of the present invention can be manufactured by a method commonly used in the industry, and can be manufactured by adding raw materials and ingredients commonly added in the industry. Additionally, the formulation of the health functional food can also be manufactured without limitation as long as it is a formulation recognized as a health functional food. Unlike general drugs, the health functional food composition of the present invention has the advantage of having no side effects that may occur during long-term use of the drug as it is made from food, and is highly portable, so it can be taken as an adjuvant to enhance the effect of improving skin moisturization. is possible.

Hereinafter, the present invention will be described in detail. Terms used in this specification, unless otherwise defined, should be interpreted as generally understood by those skilled in the art.

The drawings and examples of this specification are intended to enable those skilled in the art to easily understand and practice the present invention. Content that may obscure the gist of the invention may be omitted from the drawings and examples, and the present invention is limited to the drawings and examples. It doesn't work.

<Example>

1. Isolation of strains from mistletoe

Gangwon-do mistletoe was suspended and diluted in sterilized saline solution, and 0.1 mL of the diluted solution was taken and spread on MRS Agar (MB-M0622, Kisan Bio) medium. Afterwards, it was cultured in a constant temperature incubator at 33 to 37°C for 48 hours. Each colony generated was streaked onto an MRS agar plate and cultured at 35°C for 48 hours. Colonies that appeared white were selected to select microorganisms with a skin moisturizing effect.

2. Identification of microorganisms

As a result of analyzing the 16s rRNA base sequence of the selected strain, it was confirmed that it had the base sequence of [SEQ ID NO: 1], and as a result of analyzing the genetic phylogenetic tree, it was confirmed to show 100% homology to Lactobacillus brevis .

The present inventor named the strain as Lactobacillus brevis NEOLACTO-ND01, and it was deposited with the Biological Resources Center (KCTC) on January 12, 2023, under deposit number KCTC 15287BP.

[SEQ ID NO: 1]

CCGACGAACGCTGGCGGCATGCCTAATACATGCAAGTCGAACGAGCTTCCGTTGAATGACGTGCTTGCACTGATTTCAACAATGAAGCGAGTGGCGAACTGGTGAGTAACACGTGGGAAATCTGCCCAGAAGCAGGGGATAACACTTGGAAACAGGTGCTAATACCGTATAACAACAAAATCCGCATGGATTTTGTTTGAAAGGTGGCTTCGGCTATCACTTCTGGATGATCCCGCGGCGTATTAGTTAGTTGGTGAGGTAAAGGCCCACCAAGACGATGATACGTAGCCGACCTGAGAGGGTAATCGGCCACATTGGGACTGAGACACGGCCCAAACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGATGGAGCAATGCCGCGTGAGTGAAGAAGGGTTTCGGCTCGTAAAACTCTGTTGTTAAAGAAGAACACCTTTGAGAGTAACTGTTCAAGGGTTGACGGTATTTAACCAGAAAGCCACGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCAGGCGGTTTTTTAAGTCTGATGTGAAAGCCTTCGGCTTAACCGGAGAAGTGCATCGGAAACTGGGAGACTTGAGTGCAGAAGAGGACAGTGGAACTCCATGTGTAGCGGTGGAATGCGTAGATATATGGAAGAACACCAGTGGCGAAGGCGGCTGTCTAGTCTGTAACTGACGCTGAGGCTCGAAAGCATGGGTAGCGAACAGGATTAGATACCCTGGTAGTCCATGCCGTAAACGATGAGTGCTAAGTGTTGGAGGGTTTCCGCCCTTCAGTGCTGCAGCTAACGCATTAAGCACTCCGCCTGGGGAGTACGACCGCAAGGTTGAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCTACGCGAAGAACCTTACCAGGTCTTGACATCTTCTGCCAATCTTAGAGATAAGACGTTCCCTTCGGGGACAGAATGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATTATCAGTTGCCAGCATTCAGTTGGGCACTCTGGTGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTACACACGTGCTACAATGGACGGTACAACGAGTCGCGAAGTCGTGAGGCTAAGCTAATCTCTTAAAGCCGTTCTCAGTTCGGATTGTAGGCTGCAACTCGCCTACATGAAGTTGGAATCGCTAGTAATCGCGGATCAGCATGCCGCGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCACACCATGAGAGTTTGTAACACCCAAAGCCGGTGAGATAACCTTCGGGAGTCAGCCGTCTAAGGTGGGACAGATGATTAGGGTGAAGTCGTAACAAGGTAGCCGTAGGAGAACCTGCGGCTGGTACCCCCCCCTCTTTTTTATAAATTGGGCCCCCCCCTCCCAGTTTTATACCGGGTGCTAGTTAGAAAGAAACCCCCCCCGATACTCAACCGGGCGGCCCCCTCAATCAAGTAGCCAAATCCCATCTGAGCGCTAGGTTTTACGTGTGTTGTCTCCGCCACGTCTCTCCGCTGACAGCAAGTTCTTCTCAGAAATAACCGAAGGTTTCTCATTCGAATTAAAACGCGCCGGGGGCGTTTTTTTCTCACAAATAATGCCGACAAACGCAGCCCCCAATTATTACCCCGCGCGTGGTGAAAGAAATTTCGCGTGGGTTTTTTTGTGAAAACACCACCCACCCCTTTAGACATTTATCCTCAAGAGGTTTTT.

3. Preparation of Lactobacillus brevis NEOLACTO-ND01 culture

Lactobacillus brevis NEOLACTO-ND01 was cultured in a constant temperature incubator under the following conditions.

- Temperature 30 ~ 40℃ (35℃), pH 5.0 ~ 6.0 (5.5), 30 ~ 50 hours (48 hours), culture medium 2% (v/v) inoculation

Media composition ingredient content (%) Glucose 3~7 (5) Yeast Extract 1 to 5 (3) Dipotassium phosphate 0.1 to 0.5 (0.35) Magnesium Sulfate 0.01~0.1 (0.03) Manganese Sulfate 0.001~ 0.01 (0.007)

The strain was cultured under the above conditions, crushed using a stirred mill or high-pressure compression, and the cells were removed from the culture by centrifugation and the supernatant was filtered to obtain a Lactobacillus brevis NEOLACTO-ND01 culture.

<Experimental example>

1. Check whether the strain culture is cytotoxic

MTT Assay was performed to evaluate the cytotoxicity of the culture of the obtained Lactobacillus brevis NEOLACTO-ND01 strain on skin cells.

In the MTT assay, the MTT [3-(4,5-dimethythiasol-2-yl)-2,5-diphenyl tetrazolium bromide] reagent is absorbed into the cell and then formazan is activated by mitochondrial succinate dehydrogenase. ), and the accumulation of this substance within the cell indicates mitochondrial activity, broadly speaking, cell activity, and is a representative method of measuring cell survival.

To check the expression of each gene, cells were dispensed with 200㎕ medium into 96 wells at a density of 1Х10 ⁵ cells/well, cultured at 37°C in 5% CO ₂ for 24 hours, then discarded the medium and washed with PBS. The obtained Lactobacillus brevis NEOLACTO-ND01 culture was treated in each well at various concentrations from 0.1 wt% to 5.0 wt% and cultured for 24 hours.

4 hours before the end of incubation, 20 ㎕ of 5 mg/ml MTT dissolved in PBS was added to each well, blocked from light with aluminum foil, and cultured in an incubator under 5% CO2 and 37°C conditions for 3 hours.

The culture medium was removed, 200 ㎕ of DMSO solution was added and reacted at 37°C for 1 hour, and the absorbance was measured and compared at 570 nm using an ELISA reader. Cell viability was calculated using the formula below and the results are shown in Table 2 below. .

- Cell viability (%) = Absorbance of sample added group/Absorbance of control group Х100

division Cell viability (%) 0.1% 0.5% One% 5% culture 100 101 100 100

As can be seen from the results in Table 2 above, it was confirmed that there was no effect on cytotoxicity as all samples to which the culture was applied had high cell survival rates, and the culture of Lactobacillus brevis NEOLACTO-ND01 strain had no skin safety problems. confirmed.

2. Confirmation of increased expression of hyaluronic acid synthase (HAS-3) in strain culture

Human fibroblast NHDF was adjusted to 5Х10 ⁵ cells/well using DMDM medium supplemented with 10% FBS, then inoculated into a 12 well plate and cultured for 18 hours.

Afterwards, the obtained Lactobacillus brevis NEOLACTO-ND01 culture was treated and cultured again for 24 hours. RNA was extracted from cultured cells and the increased expression of Hyaluronan Synthase-3 (HAS-3) was confirmed through reverse transcription polymerase chain reaction (RT-PCR). Hyaluronic Acid was used as a control, and the increase rate of HAS-3 expression was calculated by the following equation, and the results are shown in Table 3.

- HAS-3 expression increase rate (%) = (sample HAS-3 expression amount / control HAS-3 expression amount) Х100

division HAS-3 expression increase rate (%) 0.1% 0.5% One% culture 82.7 85.9 91.4 Hyaluronic Acid 75.9 78.6 83.2

As can be seen from the results in Table 3, the culture confirmed that HAS-3 expression increased in a concentration-dependent manner, meaning that the culture of the Lactobacillus brevis NEOLACTO-ND01 strain exhibits a skin moisturizing effect.

3. Confirmation of the effect of inhibiting the activity of hyaluronidase in the strain culture

The effect of inhibiting hyaluronidase activity was confirmed by applying the Morgan-Elson method.

The final enzyme activity of hyaluronidase was set to 400 NF unit/ml, the final concentration of HA was set to 0.4 mg/ml, and the activator, Compound 48/80 buffer solution (0.1 mg/ml), was used to produce inactive hyaluronidase. Hyaluronidase activity was measured focusing on the inhibition of the activation step of nidase.

The obtained Lactobacillus brevis NEOLACTO-ND01 culture was mixed with a buffer solution to serve as a sample solution, and the buffer solution was used as a control group. Hyaluronic acid was used as a control. The activity inhibition rate (%) of hyaluronidase was calculated using the equation below, and the results are shown in Table 5 below.

- Hyaluronidase activity inhibition rate (%) = [(A-B)/A] x 100

A: Enzyme activity in wells without sample addition

B: Enzyme activity of the well to which the sample was added

division Hyaluronidase activity inhibition effect (%) 0.1% 0.5% One% culture 71.4 72.7 76.7 Hyaluronic Acid 73.8 74.2 75.8

As can be seen from the results in Table 4 above, the culture confirmed that hyaluronidase activity was inhibited in a concentration-dependent manner, meaning that the culture of the Lactobacillus brevis NEOLACTO-ND01 strain exhibits a skin moisturizing effect.

4. Skin safety evaluation of strain cultures

To confirm the irritation relief effect and skin safety, a human skin patch test was conducted on 30 male and female test subjects in their 20s to 50s who had never shown hypersensitivity to skin irritation in the past and had no current symptoms of skin disease or skin allergy.

To evaluate primary skin irritation by skin patch test, a skin patch test was conducted on 30 test subjects using Finn Chambers (SmartPractice®, USA). After wiping the test subject's back with 70% ethanol and drying it, 20 ㎕ of the obtained Lactobacillus brevis NEOLACTO-ND01 culture was dropped into a Finn Chamber with a diameter of 8 mm and fixed by attaching it to the test site. The patch was attached for 24 hours, and after removal, the test area was marked with a marking pen (Skin marker pen, DeRoyal, Inc., USA).

The judgment was made by observing the degree of skin irritation 30 minutes and 24 hours after the patch was removed by a dermatologist according to the judgment criteria of the International Contact Dermatitis Research Group (ICDRG) in Table 7, and the average skin reactivity was The average skin response was calculated according to the calculation formula, and the skin irritation level of the culture was finally judged according to the judgment table below.

Skin irritation index criteria (International Contact Dermatitis Research Society) sign score Criteria - 0 Negative (no stimulation) ± 0.5 Doubtful or slight reaction and erythema + One Erythema + Induration ++ 2 Erythema + Induration + Vesicle +++ 3 Erythema + Induration + Bullae

- Skin irritation index calculation formula

Skin irritation criteria skin irritation index Determination of skin irritation level 0.00 ~ 0.25 Non-irritating (excellent) 0.26 ~ 1.00 Mild irritation (good) 1.01 ~ 2.50 Moderately irritating (bad) 2.51 ~ 4.00 Strong irritant (very bad)

Test substance name 30min1 24hrs2 Average skin response Judgment result strain culture 0.00 0.00 0.00 non-irritating
(Excellent)

1: Number of skin reaction test subjects 30 minutes after patch removal

2: Number of skin reaction test subjects 24 hours after patch removal

From Table 7 above, in the case of the culture of Lactobacillus brevis NEOLACTO-ND01 strain, skin irritation was determined to be non-irritating, confirming excellent skin stability.

5. Confirmation of water retention ability of strain culture

In order to evaluate the skin moisturizing effect of the Lactobacillus brevis NEOLACTO-ND01 culture obtained above, the moisture retention ability was measured on the skin to which the culture was applied as follows.

To measure the water retention capacity, a certain amount (0.01g/4㎠) of the Lactobacillus brevis NEOLACTO-ND01 culture obtained above was applied to the inside of the forearm of 10 test subjects in a constant temperature and humidity room with a room temperature of 22°C and a relative humidity of 45%. The moisture content of the skin was measured before, 1 hour after and 2 hours after application. In the control group, the moisture content of the skin to which the culture was not applied was measured.

A moisture content meter (corneometer CM820, Courage + Khazaka electronic GmbH) was used to measure moisturizing power by measuring the electrical capacitance of the skin according to the moisture content of the skin.

culture control group Before application 55 55 1 hour after application 87 58 2 hours after application 81 53

From Table 8 above, it was confirmed that in the case of the culture of the Lactobacillus brevis NEOLACTO-ND01 strain, the electrical conductivity of the skin was higher than that of the control group. Accordingly, the culture of the Lactobacillus brevis NEOLACTO-ND01 strain was found to have excellent water retention ability and its moisturizing effect. was confirmed.

6. Confirmation of transepidermal water loss of strain culture

In order to evaluate the skin moisturizing effect of the culture of the Lactobacillus brevis NEOLACTO-ND01 strain, the amount of transepidermal water loss was measured on the skin applied and the skin not applied with the culture of the Lactobacillus brevis NEOLACTO-ND01 strain as follows. Measured.

To measure transepidermal water loss (TEWL), a certain amount (0.01g/4㎠) of the culture and control group was applied to the inside of the forearm of 10 test subjects in a constant temperature and humidity room with a room temperature of 22℃ and a relative humidity of 45%. Transepidermal water loss was measured 4 hours after and 12 hours after application.

TEWAMETER TM210 ((C+K eletronic GmhH) was used as a transepidermal water loss meter, and the skin barrier recovery rate (%) was confirmed by measuring the change in TEWL value.

Skin barrier recovery rate (%) culture Not applied 4 hours after application 90.1 76.4 12 hours after application 89.8 42.8

From Table 9 above, it was confirmed that the culture of the Lactobacillus brevis NEOLACTO-ND01 strain had a higher skin barrier recovery rate compared to the control group. Through this, the culture of the Lactobacillus brevis NEOLACTO-ND01 strain showed a skin moisturizing effect by suppressing transepidermal water loss. It was confirmed that it has.

Korea Research Institute of Bioscience and Biotechnology Biological Resources Center (KCTC) KCTC15287BP 20230112

Claims (8)

Lactobacillus brevis ( Lactobacillus brevis ) strain,
The strain is,
It is Lactobacillus brevis NEOLACTO-ND01 deposited with deposit number KCTC 15287BP,
The strain is a Lactobacillus brevis strain comprising one or more of the following activities:
i) Increased expression of intracellular hyaluronic acid synthase (HAS-3); and
ii) Inhibition of the enzymatic activity of hyaluronidase.
According to paragraph 1,
The strain is characterized in that it has the 16S rRNA sequence of SEQ ID NO: 1.
delete delete Skin comprising as an active ingredient at least one selected from the group consisting of the strain culture of any one of claims 1 and 2, concentrate of the culture, dried product of the culture, extract of the culture, and dead cells of the strain. Moisturizing cosmetic composition.
According to clause 5,
The cosmetic composition is a formulation selected from the group consisting of solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation and spray. A cosmetic composition characterized by having.
Strain culture according to any one of claims 1 and 2, behenyl alcohol, stearic acid, polysorbate 60, sorbitan sesquioleate, sorbitan stearate, self-emulsifying glycerin monostearate, squalane, propanediol, glycerin , a cream composition for moisturizing skin containing carboxypolymer, triethanolamine, 1,2 hexanediol, and purified water.
Skin comprising as an active ingredient at least one selected from the group consisting of the strain culture of any one of claims 1 and 2, a concentrate of the culture, dried material of the culture, extract of the culture, and dead cells of the strain. Moisturizing health functional food.