KR20140121606A - Cosmetic composition containing Cormus officinalis and method for manufacturing the same - Google Patents

Abstract

The present invention relates to a cosmetic composition having the effects of anti-aging, whitening, and moisturizing of a fermented Cornus officinalis extract; and a method to manufacture the same. The cosmetic composition contains the fermented Cornus officinalis extract obtained by the method comprising: collecting the flesh of a Cornus officinalis and naturally drying the flesh, and then finely grinding the dried flesh using a grinder; adding a solvent like water 10 times the weight ratio of the dried Cornus officinalis powder and extracting the extract at 25-30°C for 12-24 hours; removing microorganisms in the extract, which has been filtered with a filter cloth, using a 0.2 um filter; inoculating 5-10% of a culture fluid of Kazachstania unispora into the extract; and incubating at 20-25°C for 4-7 days, thereby being very effective in anti-aging, whitening, and moisturizing. More specifically, the cosmetic composition uses the fermented Cornus officinalis extract, promotes differentiation of stratum corneum of skin cells, recovers functions of damaged skin barrier, and has the effects of increasing in moisturizing, whitening, and anti-aging, thereby being useful as a cosmetic composition containing the fermented Cornus officinalis extract for anti-aging, whitening, and moisturizing.

Description

[0001] The present invention relates to a cosmetic composition containing Cormus officinalis and a method for manufacturing the same,

The present invention relates to a skin aging-inhibiting effect, a whitening effect, and a moisturizing cosmetic composition.

Particularly, the present invention relates to a fermented soybean oil fermented extract obtained by culturing a yeast or Kaziera unispora strain in a fermented soybean oil extract as an active ingredient, Is a non-irritating, safe skin that has no problem of inducing skin diseases, and is excellent in whitening and skin moisturizing effect. And a manufacturing method thereof

The skin is divided into three layers of epidermis, dermis and subcutaneous adipose tissue in order from the outside, and functions to protect the human body from physical and chemical stimulation of the external environment. Especially, skin carries about 65 ~ 70% of the human body's moisture, that is, various physiologically active substances necessary for the human body, and helps to keep the skin soft and moist, It plays a role.

In addition, the epidermis is divided into the stratum corneum, granular layer, superficial layer, and basal layer from the outside. The cells of the stratum corneum act like bricks, and the intercellular lipids between the keratinocytes act as cement like mortar, (J. Invest. Dermatol. 80 (Suppl.), 44-49, 1983).

Recently, the fact that not only the moisture content of the stratum corneum is increased but also the differentiation process developed from the keratinocytes of the basal layer to the keratinocytes of the outermost stratum corneum is performed normally, It turned out. In other words, physiological skin drying results in prolonged removal time of the stratum corneum, lowering lipid synthesis ability of epidermal cells, and decreasing the amount of moisturizing factor and intercellular lipid in the stratum corneum of the skin. Therefore, The maintenance function can be improved. Therefore, it can be expected that an excellent skin moisturizing effect can be obtained by using a water retaining material in the stratum corneum and a substance promoting keratinization promotion together.

The marshmallow oil compositions using the extracted mushroom oil extracts have not only a small desired moisturizing effect on the skin but also have a long time to obtain such extracts, which is not practical.

Therefore, there is a further need for development of a skin aging-inhibiting, whitening and moisturizing cosmetic composition which is stable without irritation to skin through the fermentation of corn oil-derived extract and maximized antioxidant inhibitory effect.

It is an object of the present invention, which is created to solve the above-mentioned problems, to provide a method for producing a fermented product, which comprises fermenting a fermented product of a fermented corn oil, Suppression, whitening and moisturizing effect in a short period of time, and to provide a cosmetic composition for inhibiting skin aging, whitening and moisturizing for a long time and highly safe, and a process for producing the same.

The object of the present invention is to provide a process for producing a fermented soybean oil which comprises taking the fermented soybean oil, drying it naturally and finely pulverizing it with a blender, adding a solvent such as water in an amount of 10 times the weight of the dried fermented soybean milk powder and extracting it at 25 to 30 ° C for 12 to 24 hours, The extract obtained by filtering the extract with a filter cloth was sterilized with a 0.2-μm filter and cultured at 20 to 25 ° C. for 4 to 7 days in a culture medium containing 5% to 10% of a culture medium of Kazachstania unispora to the inactivated extract. The skin aging-inhibiting effect, the whitening effect and the moisturizing effect of the fermentation liquid of the extract of corn oil-containing milk containing the extract as an active ingredient.

Preferably, the edible sour milk of the present invention is dried and extracted with distilled water to extract 10% by weight of the extract of corn oil and centrifuged to remove the precipitate to obtain a crude oil extract.

Another object of the present invention can be attained by a skin aging-inhibiting effect, a whitening effect and a moisturizing cosmetic composition of a fermented liquid of a fermented milk extract prepared by the above two methods.

INDUSTRIAL APPLICABILITY As described above, the cosmetic composition containing the fermented extract of corn oil is excellent in skin aging inhibition, whitening and moisturizing. Particularly, the cosmetic composition of the present invention uses fermented extract of corn oil, promotes the differentiation of the skin horny layer, restores the barrier function of the damaged skin, increases the skin moisturizing power, whitening effect and skin aging inhibition effect in human experiments, There is an effect that it can be effectively used as a cosmetic composition for inhibiting skin aging, whitening and moisturizing containing an extract.

1 is a graph showing the antioxidant activity of the extract of corn oil and the extract of the present invention.
FIG. 2 is a graph showing cytotoxicity against cell lines by the extract of corn oil and the extract of the present invention. FIG.
3 is a graph showing melanin production inhibitory effects of citron extract and acid fermentation fermentation extract in melanocytes.

Hereinafter, embodiments of the present invention will be described with reference to the drawings.

The cosmetic composition for inhibiting skin aging, whitening and moisturizing according to the present invention is characterized by containing a fermented extract of fermented milk as an active ingredient.

The fermented soybean oil fermented extract used in the present invention can be obtained by fermenting a fermented acid fermented milk extract with 1% to 10% culture of yeast or Kazachstania unispora culture.

The yeast is commercially produced and consumed in many countries, including the United States, as an acid and alcohol fermented milk derived from mountainous regions of northern Caucasia and consumed extensively in Russia and Eastern Europe. Kosikowski et al., 1997).

Unlike other fermented milk, Kefir is produced by using Kefir grain instead of lactic acid starter. Bacteria are contained in the outer shell of kefir grain, yeast yeast) are mixed and are symbiotic, the shape is yellowish white and popcorn-like irregular. Kazachstania unispora was used in the Life Resource Center.

The Cormus officinalis used in the present invention is said to be dried and has the flesh quality of the fruit of Sansui, which is a deciduous subfamily belonging to the Tertiary family, Functional regulation, hypoglycemia, inflammation, and antimicrobial action.

The crude powder is dried and pulverized. The pulverized powder is extracted with low temperature of 5 ~ 10 wt% in a solvent such as water and then separated by filtration. 1 ~ 10% of a capyer strain (kazachstania unispora) is inoculated into the extract, Fermented for ~ 7 days and then separated by filtration to freeze the fermented extract at -20 ° C in order to minimize heat damage to the fermented extract of fermented sour milk by freeze drying. The fermented extract of corn oil can be used in powder form.

The powdery fermented SR fermented extract obtained as described above is preferably contained in an amount of 0.001 to 30% by weight in the cosmetic composition for inhibiting skin aging, whitening and moisturizing of the present invention. When the content is 0.001% by weight or less, It is difficult to expect inhibition, whitening and moisturizing effect. When it is more than 30% by weight, there is little change in skin aging inhibition, whitening and moisturizing effect.

The cosmetic composition for inhibiting skin aging, whitening and moisturizing comprising the fermented extract of fermented sour milk of the present invention as an active ingredient is not particularly limited and may be appropriately selected according to the purpose.

For example, the cosmetic composition of the present invention may be formulated into a nutritional lotion, nutritional cream, essence, emulsion, and the like.

The nutritive cream and emulsion may be prepared so as to contain 5 to 20% by weight of oils (squalane, vegetable oil, petroleum jelly, etc.) and 3 to 15% by weight of wax components (stearyl alcohol, beeswax, etc.) , Nutritional lotion and essence can be prepared by containing 5 to 30% by weight of polyvalent alcohols (glycerin, butylene glycol, etc.) in addition to the fermented extract of fermented sour milk of the present invention.

In addition, the cosmetic composition for humidification of the present invention may further contain, in addition to the components described above, an oil, a purified water, a surfactant, a moisturizer, a lower alcohol, a thickener, a chelating agent, a pigment, a preservative, May be appropriately used in combination.

Hereinafter, the present invention will be described in more detail with reference to the following examples. However, the scope of the present invention is not limited to the following examples.

The whitening effect cosmetic composition of the present invention can be prepared in any formulations conventionally produced in the art, and examples thereof include emulsions, creams, lotions, packs, lotions and the like.

Example 1) Preparation of Corn oil extract

Dried corn syrup was added and the extract was diluted with distilled water to make 10% by weight of corn oil extract. This was centrifuged to remove sediment, and the extract of corn oil was prepared.

Example 2) Preparation of fermented extract of corn oil

The corn oil extract was sterilized by filtration with a 0.2-μm filter, and the obtained extract was inoculated with 10 ⁶ cells / ml of a carpenter strain (kazachstania unispora) at 20 to 25 ° C. for 4 to 7 days.

The fermentation broth was centrifuged at 10,000 g for 30 minutes to obtain a fermentation broth of the final fermented milk product from which polymer precipitates and fungi were removed.

The fermented extract is frozen at -20 ° C in order to minimize the heat loss of the fermented extract of fermented sour milk through freeze-drying. The fermented extract of corn oil can be used in powder form.

Example 3: Analysis of organic acids in the extracts of corn oil and fermented extracts

For the analysis of organic acids in corn oil extract and fermented extract, the extracts of corn oil and fermented corn oil were centrifuged at 16,000 rpm for 30 minutes, and the supernatant was filtered with 0.45um filter and analyzed by HPLC.

Table 1 below shows the analysis of organic acids of the fermented extract of corn oil and acid yield.

Example 4) Antioxidant activity of acidified milk extract and fermented extract

The electron donating effect was determined by modifying the Blois method and measuring the electron donating effect on 1,1-diphenyl-2-picrylhydrazyl. To 2 mL of the DPPH solution prepared at 5 mg / 100 mL concentration in ethanol, 1 mL of the sample extract with controlled concentration was added, and the mixture was reacted at room temperature for 20 minutes and absorbance was measured at 525 nm. The concentration of BHT used as a control was adjusted to 0.1%. The freezing and drying fermented extract of dried fermented milk powder were measured to have a concentration of 300 ug / ml. The results are shown in FIG.

Example 5) Whitening effect of fermented extract of corn oil

Cell culture

The cells used for the experiment are murine melanocytes of B16-F10 cell line and are a cell line which synthesizes melanin. (0.1 mg / ml, 0.5 mg / ml, 1 mg / ml, and 1.5 mg / ml) of the two materials (acid hydrolyzate, acid fermented hot water fermentation extract) were treated for 12 hours to inhibit the formation of melanin After the addition of 50 μl of CCK-8 assay solution, the reaction was carried out in a 37 ° C incubator for 3 hours at 37 ° C.

Cytotoxicity to cell line B16-F10 cell line that synthesizes melanin

The cells were cultured for 24 h, and then 50 μl of CCK-8 assay solution was added to each cell. The cells were cultured for 1, 2, 3, 4, After incubating for 3 hours in a CO2 incubator, check the OD value at the OD450nm wavelength. The OD value and the OD value of the material and CCK-8 assay solution reacted with the CCK-8 solution with the negative control were used as blank values. As a result, Is shown in FIG.

Effects of Fermented Extracts of Cornus koreanum Extracts and Fermented Soy Sauce on Melanin Formation in Melanocytes

Tyrosinase was purchased from Sigma, and the substrate, L-tyrosine (Sigma), was dissolved in 0.05 M sodium phosphate buffer (pH 6.8) and used as a 0.1 mg / ml solution. Fermented extracts of corn oil and fermented corn oil were prepared by dissolving in 0.05 M sodium phosphate buffer solution (pH 6.8) by adjusting concentration (25, 50, 100, 500, 1000, 1,500 ug / ml). 0.5 ml of L-tyrosine solution was added to a test tube and 0.5 ml of each of the extracts of corn oil and fermented extract of Sansui oil (25, 50, 100, 500, 1000, 1500 ug / ml) was added thereto and left at 37 ° C for 10 minutes.

Thereafter, 0.5 ml of 200 units / ml tyrosinase was added to the sample solution, and the mixture was reacted at 37 ° C for 10 minutes. The reaction tube was placed on ice to quench the reaction, and the absorbance of the reaction tube was measured with a spectrophotometer at a wavelength of 475 nm.

As a negative control, 0.5 ml of a buffer solution was used instead of the above sample, and arbutin, well known as a whitening agent, was used as a positive control.

The inhibition rate of tyrosinase activity of each of the above samples was calculated according to Equation (1).

The inhibition of melanogenesis in melanocytes was investigated by the addition of the extracts of corn oil and fermented extracts (25, 50, 100, 500, 1000, 1500 ug / ml). As a result, positive control arbutin (50 ug / The more potent inhibitory effect of melanin formation is shown in Fig.

Example 6) Formulation Example (lotion formulation)

In the composition shown in Table 2 below, the solubilized portions were premixed and dissolved in a transparent state, and then the mixture was stirred with a 20 rpm Paddle Mixer while gradually adding the aqueous solution to the aqueous mixture.

Examples of the humectant include glycerin, butylene glycol, and sodium hyaluronate, as well as phage-based polyhydric alcohols and various saccharides (sorbitol, xylitol, glucose, mannan, etc.) and urea.

As an incremental agent, carbomers, acrylic polymers, cellulose derivatives, glucoproteins (mucin, heparin, chondroitin sulfate, etc.) and clay-based dispersion stabilizers (bentonite, magnesium aluminum silicate, etc.) may be used in addition to xanthan gum.

As the solubilizing agent, various hydrophilic surfactants (HLB 16 or more) can be used in addition to the above PEG hydrogene decanter oil.

Example 7) Formulation Example (nutritional cream or emulsion formulation)

In the following Table 3, the oil phase and the water phase were completely dissolved at 75 ° C, emulsified at 4,000 rpm for 5 minutes, and cooled to prepare a cream formulation at 30 ° C.

However, corn oil extract or fermented extract of corn oil was added at 45 캜.

As the vegetable oil, sesame oil, macadamia nut oil, olive oil, camellia oil, wheat germ oil, rape seed oil, squalane and jojoba oil can be used singly or as a mixture. Acetate) can be used in small amounts.

As the emulsifier, various nonionic hydrophilic surfactants + nonionic hydrophilic surfactants may be combined with HLP 10 - 12 groups in addition to the above polysorbate and sorbitan sesquioleate, and the sum thereof may be used in the range of about 2 to 4 have.

In addition to triethanolamine as an alkali agent, potassium hydroxide, sodium hydroxide, and the like can be used, and when a carbomer or the like is used as an increasing agent, arginine can also be used.

Example 8) Formulation Example (Essence formulation)

In Table 4, the solubilized portions were premixed and dissolved in a transparent state, and then agitated with a 20 rpm Paddle Mixer while gradually adding the aqueous solution to the aqueous mixture.

Examples of the humectant include glycerin, butylene glycol, and sodium hyaluronate, as well as phage-based polyhydric alcohols and various saccharides (sorbitol, xylitol, glucose, mannan, etc.) and urea.

In addition to xanthan gum, a thickening agent such as a carbomer, an acrylic polymer, a cellulose derivative, a glucoprotein (mucin, heparin, chondroitin sulfate, etc.), a dispersion stability enhancer of clay component (bentonite, magnesium aluminum silicate, etc.) Alginine or triethanolamine can then be added as an alkaline agent.

As the solubilizing agent, various hydrophilic surfactants (HLB 16 or more) can be used in addition to the above PEG hydrogene decanter oil.

While the present invention has been described with reference to exemplary embodiments, it is to be understood that the invention is not limited to the disclosed exemplary embodiments.

Claims (3)

Dried naturally and pulverized finely with a blender, but the solvent such as water is added 10 times with respect to the weight ratio of dried corn oil powder and extracted at a temperature of 25 to 30 ° C for 12 to 24 hours. The extract is filtered with a filter cloth The extract was sterilized with a 0.2-μm filter, and 5% to 10% of a culture medium of Kazachstania unispora was added to the inactivated extract, and cultured at 20 to 25 ° C for 4 to 7 days. The skin aging inhibitory effect, whitening efficacy and moisturizing effect of fermented milk extract of corn oil.
The method according to claim 1,
Wherein the acidified milk is dried and extracted with distilled water to extract 10% by weight of the extract of corn oil, and the precipitate is removed by centrifugation to obtain a fermented milk extract, wherein the fermented milk extract has an inhibitory effect on skin aging, a whitening effect and a moisturizing effect A method for producing an effective cosmetic composition.
A cosmetic composition for inhibiting skin aging, whitening efficacy and moisturizing effect of a fermented liquid of the extract of corn oil produced by the method according to claim 1 or 2.

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