KR101641613B1 - Skin whitening or skin moisture cosmetic composition with fermented Schizandra chinensis by Tricholomopsis rutilans or the extract therefrom - Google Patents

Abstract

The present invention relates to a cosmetic composition for skin whitening and skin moisturizing which contains, as an active ingredient, a fermentation broth obtained by fermenting an extract of Omija with mycelium of Solfusa or an extract thereof, and the cosmetic composition of the present invention has excellent skin whitening ability and skin moisturizing ability .

Description

[0001] The present invention relates to a cosmetic composition for skin whitening or skin moisturizing, which comprises an extract of Schizandra chinensis or an extract thereof,

More particularly, the present invention relates to a cosmetic composition comprising a fermented broth of Omija sol mushroom or an extract thereof as an active ingredient, and more specifically, to a cosmetic composition comprising an extract of Omija solum mushroom fermentation broth obtained by fermenting an extract of Omija with a mushroom mycelium The present invention relates to a cosmetic composition for skin whitening and skin moisturizing.

Solar ultraviolet radiation (UV) is involved in the proliferation and differentiation of skin cells. UV-induced effects in the skin are accompanied by direct DNA damage, apoptosis, growth arrest, and excessive melanogenesis. In addition, long-term exposure to UV causes photoaging and photocarcinogenesis, and epidermal melanocyte synthesizes two major melanins, eumelanin and pheomelanin. Melanin inhibits UV penetration through the epidermal layer and effectively removes the radicals of reducing oxygen induced by oxidative DNA damage.

On the other hand, attempts to develop new whitening agents are still being carried out, and development of evaluation methods and material development techniques have led to the development of different raw materials for various mechanisms. It is known that arbutin, licorice extract and mulberry extract inhibit the activity of tyrosinase which contributes to melanin synthesis. Melasolv is known to inhibit the expression of tyrosinase, Vitamin C and its derivatives, Coenzyme Q10, are known to block the synthesis of melanin. In addition, raw materials that block the movement of the resulting melanomas to keratinocytes have been developed.

On the other hand, the skin is divided into three layers of epidermis, dermis, and subcutaneous tissue in order from the outside, and functions to protect the internal organs from changes in temperature and humidity, ultraviolet rays and other physical and chemical external environment have. Among them, the epidermis plays an important role in preventing water evaporation inside the human body.

The epidermis is divided into the stratum corneum, the granular layer, the superficial layer and the basal layer in order from the outside. Cells of the stratum corneum act as bricks, and the intercellular lipids between the keratinocytes function as mortar to form skin barrier J. Invest. Dermatol 80 (Suppl.), 44-49, 1983). In healthy human keratinocytes, a high concentration of Natural Moisturing Factor (NMF) is present to help maintain moisture in the skin. For example, substances such as amino acids are water-soluble and thus effectively combine with moisture to dry the skin (J. Invest. Dermatol. 54, 24-31, 1970).

On the other hand, industrialization due to the development of science has caused a drastic change in the global environment, which has had a serious impact on human health. As a result, various chronic and intractable diseases are rapidly increasing, and the incidence of environmental diseases such as atopic dermatitis, allergic rhinitis and asthma is increasing worldwide.

In order to prevent the aforementioned environmental diseases, skin moisturization is indispensable. In the past, humectants having a property of absorbing moisture as a moisturizing agent and occlusive moisturizers preventing evaporation of water have been used, and moisture content in the stratum corneum Has been performed. Examples of humectant include glycerin, propylene glycol, 1,3-butylene glycol, polyethylene glycol, sorbitol, 2-pyrrolidone-5-carboxylate and the like. This drawback has its drawbacks. (5), 731-740, 1994), there is a difficulty in maintaining the stability of the emulsified formulations, and there is a problem in that a transparent gel phase There is a disadvantage that it is not suitable for manufacturing a product.

On the other hand, Schizandra chinensis BAALL. ( Schizandra chinensis BAALL.) Is a plant that grows in the mountain valleys of various places, especially all-stone place (round stone where there is no rounded round stones). It is wide oval, long oval Or ovate. Leaf length is 7 ~ 10cm, width is 3 ~ 5cm, there are small teeth on the edge. The flower is bloomed in June to July, with a reddish orange-red color. Fruits ripen in red in August, spherical or 倒 卵 shaped, spherical, 6 ~ 12 mm long and contain one or two seeds. This fruit is called Omiza because it has five flavors of sweet, light, spicy, spicy and salty, and the sour taste is strongest among them. Malic acid and tartaric acid are known as sour taste ingredients, and they are used as medicines in oriental medicine. In animal experiments, the cerebral nerves were excited and showed a tonic effect, and it is known to act directly on respiratory addiction. In addition, it was recognized that blood pressure was regulated by supporting cardiac activity, and hepatic metabolism was promoted by entering the liver. In the case of extracts using omija, it is known that isostannin, gyminin A, and hyperinsin N are effective as whitening substances, but they are not available because they can not give a substantial effect because they contain a small amount.

The size of the gill mushroom ( Tricholomopsis rutilans (Schaeff.:Fr.) Sing.) Is 4 ~ 20 cm in diameter and gradually becomes flat in the bell shape. The yellow background of the surface is covered with a dark red scaly sculpture, and you can feel soft leather feeling when you touch it. The wrinkles are yellow and dense with the right wrinkles or grooved wrinkles. There is a fine powder on the edges of the wrinkles. The length of the bag is 6 to 20 cm, and the thickness is 1 to 2.5 cm. Fine blooms are scattered on a yellow background with a little thin under the bag. The genus is flesh. The size of the spores is 5.5 ~ 7 × 4 ~ 5.5 μm and short elliptical. It grows into a stubby stump of deciduous trees and rotten trees, especially stumps of pine trees, from summer to autumn.

On the other hand, there has not been disclosed a technique for increasing the content of isostannin, hypermucin A and hypermucin N components of the extract of Omiza, and in particular, whitening and moisturizing effects of the fermentation broth obtained by fermenting Omiza extract with a mycelia of Solfushi, There is no way.

KOKAI Publication No. 10-2013-0087938 (Registration Date: 2013.08.07) relates to a cosmetic composition for improving wrinkles containing an extract of Omija, wherein a cosmetic characterized by containing an Omija extract as an active ingredient .

The present invention provides a natural extract which is applicable to skin cosmetics and which is harmless to human body and which is highly safe, and a cosmetic composition containing the same as an active ingredient.

It is another object of the present invention to provide a cosmetic composition for skin whitening or skin moisturizing which contains a fermentation liquid obtained by fermenting Omiza extract with a mycelial extract of Solanaceae as an active ingredient.

It is another object of the present invention to provide a cosmetic composition for skin whitening or skin moisturizing, which comprises an extract of a fermentation broth obtained by fermenting an extract of Omija with a mycelia of Solanum mushroom as an active ingredient.

In order to achieve the above object, the present invention provides a cosmetic composition comprising, as an active ingredient, a fermentation broth obtained by fermenting an extract of Omija with a mycelium of Solfusa as a first form.

The above-mentioned Omija extract is preferably extracted using any one of extraction solvents selected from the group consisting of water, an anhydrous or a lower alcohol having 1 to 4 carbon atoms, acetone, ethyl acetate, butyl acetate and 1,3-butylene glycol . Among the above solvents, alcohols are preferable, and ethanol is more preferable. At this time, the ethanol is preferably 70% ethanol. The suitable amount of the extraction solvent is 1 to 15 times, more preferably 5 to 10 times, and most preferably 7 times the dry weight of Omija.

The fermentation broth may be one obtained by inoculating a mycelium of Solanum officinale with an extract of Schizandra chinensis, followed by fermentation for 5 to 10 days while maintaining a pH of 5 to 7 at 35 to 40 ° C.

The fermentation broth is preferably added in an amount of 0.0001 to 30.0% by weight based on the total weight of the cosmetic composition. More preferably from 0.001 to 15.0% by weight, and most preferably from 0.1 to 10% by weight. If the added amount is less than 0.0001% by weight, the effect is insignificant. If the added amount is more than 30.0% by weight, the effect of increasing the content is not increased, which is not economical.

In addition, the cosmetic composition may preferably be used for skin whitening.

In addition, the cosmetic composition may preferably be used for skin moisturizing.

On the other hand, the second aspect of the present invention provides a cosmetic composition comprising as an active ingredient an extract of a fermentation broth obtained by fermenting an extract of Omija with a mycelia of Solfusa.

The above-mentioned Omija extract is preferably extracted using any one of extraction solvents selected from the group consisting of water, an anhydrous or a lower alcohol having 1 to 4 carbon atoms, acetone, ethyl acetate, butyl acetate and 1,3-butylene glycol . Among the above solvents, alcohols are preferable, and ethanol is more preferable. At this time, the ethanol is preferably 70% ethanol. The suitable amount of the extraction solvent is 1 to 15 times, more preferably 5 to 10 times, and most preferably 7 times the dry weight of Omija.

Preferably, the extract of the fermentation broth is inoculated with a mushroom mycelia to an extract of Omija, fermented for 5 to 10 days while maintaining a pH of 5 to 7 at 35 to 40 ° C, And may be extracted by adding an 80% (v / v) aqueous solution of ethanol.

The extract of the fermentation broth is preferably added in an amount of 0.0001 to 30.0% by weight based on the total weight of the cosmetic composition. More preferably from 0.001 to 15.0% by weight, and most preferably from 0.1 to 10% by weight. If the added amount is less than 0.0001% by weight, the effect is insignificant. If the added amount is more than 30.0% by weight, the effect of increasing the content is not increased, which is not economical.

In addition, the cosmetic composition may preferably be used for skin whitening.

In addition, the cosmetic composition may preferably be used for skin moisturizing.

Meanwhile, the cosmetic composition of the present invention may contain components commonly used in cosmetic compositions in addition to the fermentation broth or the extract of the fermentation broth. For example, conventional adjuvants such as antioxidants, stabilizers, solubilizers, vitamins, pigments and flavoring agents, and carriers may be included.

On the other hand, the cosmetic composition of the present invention can be prepared into any formulation conventionally produced in the art. For example, it can be formulated as a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation and spray. More specifically, it can be manufactured in the form of a flexible lotion, a convergent lotion, a nutritional lotion, a nutritional cream, a massage cream, an essence, an eye cream, a cleansing cream, a cleansing foam, a cleansing water, a pack, a spray or a powder.

When the formulation of the cosmetic composition of the present invention is a paste, a cream or a gel, an animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide Can be used.

When the formulation of the cosmetic composition of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component, Propane / butane, or dimethyl ether. ≪ RTI ID = 0.0 >

When the formulation of the cosmetic composition of the present invention is a solution or an emulsion, a solvent, a dissolving agent or an emulsifying agent is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate , Propylene glycol, 1,3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan fatty acid esters.

When the formulation of the cosmetic composition of the present invention is in the form of a suspension, the carrier component may include water, a liquid diluent such as ethanol or propylene glycol, ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester Suspending agent, microcrystalline cellulose, aluminum metahydroxide, bentonite, agar or tracant, etc. may be used.

When the formulation of the cosmetic composition of the present invention is a cleansing agent containing an interfacial active agent, the carrier component may include aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, Fatty acid amide ether sulfate, alkylamido betaine, aliphatic alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, lanolin derivative, or ethoxylated glycerol fatty acid ester.

The present invention can provide a cosmetic composition for skin whitening and skin moisturizing which contains a fermentation broth obtained by fermenting Omiza extract with a mycelium of Solfusa as an active ingredient.

In addition, the present invention can provide a cosmetic composition for skin whitening and skin moisturizing, which comprises an extract of a fermentation broth obtained by fermenting Omiza extract with Mycelium of Solanum mushroom as an active ingredient.

Fig. 1 shows the results of confirming the inhibitory effect of tyrosinase on the extract of fermented Schizosaccharomyces mushroom (Preparation Example 3).
Fig. 2 shows the results of confirming the melanin synthesis inhibitory effect of the fermented extract of Omija sol mushroom (Preparation Example 3).

Hereinafter, the present invention will be described in more detail with reference to the following examples. However, the scope of the present invention is not limited to the following embodiments, and includes modifications of equivalent technical ideas.

[Preparation Example 1: Preparation of Omija extract]

Omija was washed with purified water, dried and then pulverized and made fine using a sieve of 100 mesh. 70% ethanol was added thereto so as to have a concentration of 150 g / L, and the mixture was refluxed three times for 5 hours. After cooling, the mixture was filtered through Whatman # 3 filter paper, And dried to produce an Omija extract.

[Preparation Example 2: Preparation of a fermentation broth of Schizosaccharomyces mushroom]

1 to 4% of glucose was added as a carbon source to 5 g of the Omiza extract prepared in Preparation Example 1, and then 0.2 to 0.5% of peptone was further added to sterilize. To this, 25 g / L of a mushroom ( Tricholomopsis rutilans (Schaeff.:Fr.) Sing.) Strain was inoculated and cultured. The cultures were incubated for 7 days at a pH of 5-7 at 37 ° C using a 5 L fermenter. After the incubation, the culture broth was centrifuged to remove the culture broth first, and the broth was sterilized (121 ° C, 15 minutes, 1.5 atm) to prevent further culture, thereby preparing a fermentation broth (hereinafter referred to as "broth of Omija solfumi").

[Preparation Example 3: Preparation of Extract of Schizosaccharomyces mellifera Fermentation Liquid]

After removing the culture of the fermentation broth prepared in Preparation Example 2, ethanol was added to make a final 70% (v / v) ethanol aqueous solution, and the mixture was refluxed three times for 5 hours. After cooling, ) # 3 filter paper. After the filtration was completed, the extract obtained in the above extraction step was transferred to a concentration tank and concentrated under reduced pressure or freeze-dried at 50 ° C or lower to prepare an extract of Schizosaccharomyces mushroom fermentation broth (hereinafter referred to as "Schizosaccharomyces fermentation extract").

[Comparative Examples 1 to 4: Preparation of Omija fermentation extract]

In this Comparative Example, each of the Omija fermented extracts was prepared using the mushroom mycelium described in Table 1 below. The production method was the same as that of Production Example 3 above.

mycelium Comparative Example 1 Red duck mushroom Comparative Example 2 Avi mushroom Comparative Example 3 Mushroom mushroom Comparative Example 4 Mulberry mushroom

[Experimental Example 1: Comparison of the amounts of iszanthin, hyperminus A and hyperinsin N of Omiza extract and Omija fermented extract]

In this Experimental Example, in order to compare the amounts of iszanthrin, hypermynx A and hypermishin N of the Omija extract of Preparation Example 1, the fermentation extract of Omija mushroom fermentation extract of Preparation Example 3, and the Omija fermentation extract of Comparative Examples 1 to 4, Respectively. The experimental methods and conditions were as follows, and the results are shown in Table 3.

<HPLC Conditions>

Approximately 0.1 g of each of the above extracts was precisely weighed and dissolved in methanol to make 100 ml, which was used as a sample solution. Separately, 10 mg of isostannin (C ₂₄ H ₃₂ O ₇ : 432.51), hyperinsin A (C ₂₃ H ₂₈ O ₇ : 416.46) and hyperinsin N (C ₂₃ H ₂₈ O ₆ : 400.47) Dissolve 100 ml of this solution, add exactly 1 ml of this solution, add methanol to make 100 ml, and use this solution as the standard solution. 20 μl of the sample solution and the standard solution were tested according to the high performance liquid chromatography method under the following conditions and then the peak areas A _TGs , A _TGA , and A _TGN of the sample solution and the peak areas A _SGS , A _SGA , and A _SGN were measured.

[Equation 1]

&Quot; (2) &quot;

&Quot; (3) &quot;

<Operating Conditions>

    Detector: Ultraviolet absorptiometer (measuring wavelength: 254 nm)

    Column: A stainless steel tube having an inner diameter of about 4.6 mm and a length of about 250 mm and filled with octadecylsilylated silica gel for a 5 탆 high performance liquid chromatograph

    Temperature: 40 ℃

    Mobile phase: Table 2

Time (min) Flow (ml / min) ACN (%) Water (%) 1.00 60.0 40.0 20 1.00 60.0 40.0 23 1.00 80.0 20.0 38 1.00 80.0 20.0 40 1.00 60.0 40.0 45 1.00 60.0 40.0

Hesandin (ppm) High myosin A (ppm) High myosin N (ppm) Production Example 1 95.2 28.2 8 Production Example 3 980 301 93 Comparative Example 1 150 47.2 12.1 Comparative Example 2 110 37.2 9.3 Comparative Example 3 99.7 28.6 8.2 Comparative Example 4 120 30.2 9.7

As a result, the component content of the fermented extract of Omija was higher than that of the fermented extract of Omija, and the content of isostearin, hypermucin A and hypermysine N in the fermented extract of the myrtle fermentation extract (Preparation Example 3) Which is about 10 times higher than that of the conventional method.

From the above results, it was found that the mycelia of Solfuscus increased biosynthesis, biosynthesis, histamine, and hyperinsin N during the fermentation process.

[Experimental Example 2: Evaluation of cell viability of Omija fermented extract]

 In this Example, the toxicity of the Omiza extract of Preparation Example 1, the fermentation extract of Omija mushroom of Preparation Example 3, and the Omija fermentation extract of Comparative Examples 1 to 4 was measured.

Cytotoxicity was performed by modifying Mosmann's method of measuring cell viability using 3- (4,5-dimethylthiazol-2-yl) -2-5-diphenyltetrazolium bromide (MTT) reagent.

HDF was added to a 96-well plate at a concentration of 1 × 10 ⁴ cells / well and cultured at 37 ° C. and 5% CO ₂ for 24 hours. After the culture, the culture medium was removed and each sample was cultured for 24 hours in a medium treated with concentrations (0.001, 0.002, 0.005, 0.01%). After incubation, the medium was removed and washed twice with PBS (phosphate buffered saline). After that, MTT was dissolved in PBS at 5 mg / ml, and 50 L was added. Then, the cells were cultured at 37 ° C and 5% CO ₂ for 2 hours. Thereafter, 100 L of DMSO was added per well, and the mixture was stirred for 10 minutes, and the absorbance was measured at 540 nm.

As a result of measurement, all the samples showed no cytotoxicity at all concentrations.

From the above results, it was confirmed that the extract of Omija mushroom fermentation of the present invention is harmless to the human body and has excellent safety.

[Experimental Example 3: Confirmation of inhibitory effect of tyrosinase of Schizosacchia mushroom fermentation extract]

In this Experimental Example, the tyrosinase inhibitory effect of the Omija extract of Preparation Example 1, the Omija mushroom fermentation extract of Preparation Example 3, and the Omija fermentation extract of Comparative Examples 1 to 4 was examined.

The enzyme, Tyrosinase (EC 1.14.18), was dissolved in 0.05 M phosphate buffer (pH 6.5) to give a final volume of 1,100 units / ml. Substrate L -Tyrosine (C ₉ H ₁₁ NO ₃ , 181.19) was dissolved in 0.1 M phosphate buffer (pH 6.5) to a final concentration of 1.55 mM (225 μM), and then 0.1 M sodium phosphate buffer, pH 6.5), and the samples were added at different concentrations, followed by reaction at 37 ° C for 20 minutes (see Table 4 below). After the reaction, the absorbance was measured at 492 nm using an ELISA reader. Meanwhile, tyrosinase inhibition rate was calculated using the following equation (4), and 50 mu M kojic acid was used as a control group.

Material Ctrol group Blank of C Exp. Group Blank of E A Buffer 140 150 140 150 B Sample (solvent) Solvent 20 Solvent 20 20 20 C Enzyme 10 - 10 - D Substrate 30 30 30 30

(Unit: μl)

&Quot; (4) &quot;

(%) = [(C-D) (A-B)] / (C-D) 100

A: Abs of experimental group

B: Abs of blank of experimental group

C: Abs of control group

D: Abs of blank of control group

As a result of the experiment, it was confirmed that the fermented extract of Omija sol mushroom of the present invention exerts the most excellent tyrosinase inhibitory ability (FIG. 1).

From the above results, it can be seen that there is an excellent skin whitening effect of the fermented extract of Omija sol mushroom of the present invention.

[ Experimental Example  4: Omiza Mushroom  Confirmation of melanin synthesis inhibitory effect of fermented extract]

In this experimental example, the effect of suppressing melanin synthesis of the Omija extract of Preparation Example 1, the fermentation extract of Omija mushroom of Preparation Example 3, and the Omija fermentation extract of Comparative Examples 1 to 4 was examined.

Melanocyte-forming cells (B16F1) were washed with PBS, detached from the culture dish using trypsin, and counted using a hematocytometer. Cells were plated at 6 × 10 ⁵ cells / well in a 6-well plate and stabilized for 24 hours. On the next day, each sample was treated after confirming by microscope whether cells were evenly distributed in the culture dish. Each sample was treated with a concentration (0.001, 0.002, 0.005, 0.01%) indicating 100% survival rate in the previous toxicity test and cultured in a CO ₂ incubator at 37 ° C for 48 hours. Cells treated with the sample and incubated for 48 hours were washed with PBS and detached from the culture dish using trypsin. Then, all the cells were collected and centrifuged at 2000 rpm for 5 minutes to precipitate. Then, 1 ml of PBS was added thereto, followed by vortexing and centrifugation at 2000 rpm for 5 minutes. After removal of the PBS, the cells were then lysed with 200 [mu] l of homogenization buffer (50 mM sodium phosphate pH 6.5, 1% Triton X-100). At this time, the solution was sufficiently dissolved in a vortex for about 30 minutes. After centrifugation at 2000 rpm for 10 minutes, the supernatant was removed to quantify the protein [BCA assay kit]. Then, 200 μl of 1 N NaOH (+ 10% DMSO) was added to the precipitate below, and the value of OD ₄₀₅ was measured at 405 nm after vortexing. At this time, the control (control) uses commercially available melanin. The OD ₄₀₅ value was determined by concentration at a concentration of 1 mg / ml, and a standard curve was prepared. Then, using this, the amount of melanin was calculated by the OD ₄₀₅ value of the sample, and the calculated value was divided by the value obtained by quantifying the protein.

As a result of the experiment, it was confirmed that the extract of Omija mushroom fermentation extract of the present invention exerts the best inhibitory effect on melanin synthesis (FIG. 2).

From the above results, it can be seen that there is an excellent skin whitening effect of the fermented extract of Omija sol mushroom of the present invention.

[Examples 1 to 2: Preparation of cosmetic preparation containing Schizosaccharomyces mushroom fermentation broth or Schizosaccharomyces mellifera fermentation extract]

A cosmetic composition containing 10.0% by weight of the fermentation broth of Omija sol mushroom of Preparation Example 2 or the 30% fermented extract of Omija sol mushroom of Preparation Example 3 was prepared with the composition shown in Table 5 below. On the other hand, a cosmetic composition not containing a fermented broth of Omija sol mushroom or a fermented extract of Omija sol mushroom was prepared and referred to as Comparative Example 1.

ingredient Example 1
Content (% by weight) Example 2
Content (% by weight) Comparative Example 1
Content (% by weight) Production Example 2 10.0 - - Production Example 3 - 10.0 - 1,3-butylene glycol 10.0 10.0 10.0 glycerin 5.1 5.1 5.1 Propylene glycol 4.2 4.2 4.2 Tocopheryl acetate 3.0 3.0 3.0 Liquid paraffin 4.6 4.6 4.6 Triethanolamine 1.0 1.0 1.0 Squalane 3.1 3.1 3.1 Macadamia nut oil 2.5 2.5 2.5 Polysorbate 60 1.6 1.6 1.6 Sorbitan sesquiterate 1.6 1.6 1.6 Propylparaben 0.6 0.6 0.6 Carboxyl vinyl polymer 1.5 1.5 1.5 incense a very small amount a very small amount a very small amount antiseptic a very small amount a very small amount a very small amount Purified water Balance Balance Balance system 100 100 100

[Experimental Example 5: Confirmation of improving effect of skin moisturizing ability of cosmetic composition containing Schizosacchus mushroom fermentation extract]

In this Experimental Example, the effect of enhancing the skin moisturizing ability of the cosmetic composition containing the extract of Omija mushroom fermented extract prepared in Example 2 was examined.

Twenty twenty to forty persons without skin disease were divided into two groups of 20 persons per one group, and the cosmetic compositions of Example 2 and Comparative Example 1 were applied to the face and forehead portions by each group. The skin conductivity was measured by a corneometer (CM820 courage Khazaka electronic GmbH, Germany) in a constant temperature and humidity condition (24 ° C, 40% humidity) The rate of increase (%) of the skin conductivity after the main course was measured and the rate of increase was evaluated. The results are shown in Table 6 below.

sample After 1 week after 2 weeks After 4 weeks Example 2 57 60 66 Comparative Example 1 25 29 34

As a result of the experiment, it was confirmed that the skin cosmetic composition containing the omelet extract of fermented mushroom of the present invention had a much higher rate of skin conductivity increase than the cosmetic composition of Comparative Example 1 (FIG. 2).

In general, the skin conductivity is proportional to the skin moisture content. Therefore, the above results show that the cosmetic composition containing the extract of the mushroom fermented with mushroom has high skin moisture content and exerts excellent skin moisturizing effect.

Claims (10)

delete delete delete delete delete A cosmetic composition characterized by containing an extract of a fermentation broth obtained by fermenting an Omija extract with a mycelia of Solfusa as an active ingredient.
The method according to claim 6,
The above-
A cosmetic composition characterized by being extracted using any one extraction solvent selected from the group consisting of water, anhydrous or lower alcohol having 1 to 4 carbon atoms, acetone, ethyl acetate, butyl acetate and 1,3-butylene glycol .
The method according to claim 6,
The extract of the fermentation broth may contain,
After 5 to 10 days of fermentation, the culture was removed, and 60 ~ 80% (v / v) aqueous ethanol solution was added to the extract. The cosmetic composition according to claim 1,
The method according to claim 6,
In the cosmetic composition,
Wherein the cosmetic composition is for skin whitening.
The method according to claim 6,
In the cosmetic composition,
Wherein the composition is for moisturizing the skin.

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