KR102428463B1 - 세포에 물질을 주입하는 장치 및 제조 방법 - Google Patents

세포에 물질을 주입하는 장치 및 제조 방법 Download PDF

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KR102428463B1
KR102428463B1 KR1020150178130A KR20150178130A KR102428463B1 KR 102428463 B1 KR102428463 B1 KR 102428463B1 KR 1020150178130 A KR1020150178130 A KR 1020150178130A KR 20150178130 A KR20150178130 A KR 20150178130A KR 102428463 B1 KR102428463 B1 KR 102428463B1
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passage
cell
cells
substance
injected
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이상현
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주식회사 펨토바이오메드
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Priority to EP15202528.4A priority Critical patent/EP3037518B1/en
Priority to ES15202528T priority patent/ES2808828T3/es
Priority to DK15202528.4T priority patent/DK3037518T3/da
Priority to PCT/KR2015/014292 priority patent/WO2016108526A1/en
Priority to JP2017535710A priority patent/JP6883517B2/ja
Priority to US14/980,682 priority patent/US10131867B2/en
Publication of KR20160079659A publication Critical patent/KR20160079659A/ko
Priority to US16/151,935 priority patent/US20190032000A1/en
Priority to US16/677,540 priority patent/US20200071652A1/en
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/42—Apparatus for the treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N13/00—Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502707—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the manufacture of the container or its components
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/02—Electrical or electromagnetic means, e.g. for electroporation or for cell fusion
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/04—Mechanical means, e.g. sonic waves, stretching forces, pressure or shear stimuli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0896—Nanoscaled
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0415—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0463—Hydrodynamic forces, venturi nozzles

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  • Apparatus Associated With Microorganisms And Enzymes (AREA)
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  • Gynecology & Obstetrics (AREA)
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  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
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  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
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  • General Physics & Mathematics (AREA)
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KR1020150178130A 2014-12-28 2015-12-14 세포에 물질을 주입하는 장치 및 제조 방법 Active KR102428463B1 (ko)

Priority Applications (8)

Application Number Priority Date Filing Date Title
ES15202528T ES2808828T3 (es) 2014-12-28 2015-12-23 Dispositivo para colocar material en célula
DK15202528.4T DK3037518T3 (da) 2014-12-28 2015-12-23 Indretning til at indsætte materiale i celle
EP15202528.4A EP3037518B1 (en) 2014-12-28 2015-12-23 Device for putting material into cell
JP2017535710A JP6883517B2 (ja) 2014-12-28 2015-12-24 細胞に物質を注入する装置および製造方法（Ｄｅｖｉｃｅ ｆｏｒ Ｐｕｔｔｉｎｇ Ｍａｔｅｒｉａｌ ｉｎｔｏ Ｃｅｌｌ）
PCT/KR2015/014292 WO2016108526A1 (en) 2014-12-28 2015-12-24 Device for putting material into cell
US14/980,682 US10131867B2 (en) 2014-12-28 2015-12-28 Device for putting material into cell
US16/151,935 US20190032000A1 (en) 2014-12-28 2018-10-04 Device for putting material into cell
US16/677,540 US20200071652A1 (en) 2014-12-28 2019-11-07 Method and apparatus for controlling delivery of material into cells

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR1020140191302 2014-12-28
KR20140191302 2014-12-28

Publications (2)

Publication Number Publication Date
KR20160079659A KR20160079659A (ko) 2016-07-06
KR102428463B1 true KR102428463B1 (ko) 2022-08-03

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KR1020150178130A Active KR102428463B1 (ko) 2014-12-28 2015-12-14 세포에 물질을 주입하는 장치 및 제조 방법
KR1020150178234A Ceased KR20160079661A (ko) 2014-12-28 2015-12-14 운반체의 사용 없이 세포에 물질을 주입하여 변환시킨 세포
KR1020150178183A Active KR102428464B1 (ko) 2014-12-28 2015-12-14 세포에 물질을 주입하여 세포를 변환시키는 방법

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KR1020150178234A Ceased KR20160079661A (ko) 2014-12-28 2015-12-14 운반체의 사용 없이 세포에 물질을 주입하여 변환시킨 세포
KR1020150178183A Active KR102428464B1 (ko) 2014-12-28 2015-12-14 세포에 물질을 주입하여 세포를 변환시키는 방법

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US (1) US20160272961A1 (enExample)
JP (3) JP6883517B2 (enExample)
KR (3) KR102428463B1 (enExample)
DK (1) DK3037518T3 (enExample)
ES (1) ES2808828T3 (enExample)

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EP3037518B1 (en) 2014-12-28 2020-05-06 Femtobiomed Inc. Device for putting material into cell
CN108251268A (zh) * 2016-12-29 2018-07-06 上海新微技术研发中心有限公司 一种基因转染器及基因转染方法
JP2020530979A (ja) 2017-06-30 2020-11-05 インスクリプタ， インコーポレイテッド 自動細胞処理方法、モジュール、機器及びシステム
US10738327B2 (en) 2017-08-28 2020-08-11 Inscripta, Inc. Electroporation cuvettes for automation
JP6985508B2 (ja) 2017-09-30 2021-12-22 インスクリプタ， インコーポレイテッド フロースルーエレクトロポレーション機器編成
US10443031B1 (en) 2018-03-29 2019-10-15 Inscripta, Inc. Methods for controlling the growth of prokaryotic and eukaryotic cells
WO2019200004A1 (en) 2018-04-13 2019-10-17 Inscripta, Inc. Automated cell processing instruments comprising reagent cartridges
US10557216B2 (en) 2018-04-24 2020-02-11 Inscripta, Inc. Automated instrumentation for production of T-cell receptor peptide libraries
WO2019209926A1 (en) 2018-04-24 2019-10-31 Inscripta, Inc. Automated instrumentation for production of peptide libraries
US10858761B2 (en) 2018-04-24 2020-12-08 Inscripta, Inc. Nucleic acid-guided editing of exogenous polynucleotides in heterologous cells
CA3108767A1 (en) 2018-06-30 2020-01-02 Inscripta, Inc. Instruments, modules, and methods for improved detection of edited sequences in live cells
US11142740B2 (en) 2018-08-14 2021-10-12 Inscripta, Inc. Detection of nuclease edited sequences in automated modules and instruments
US10752874B2 (en) 2018-08-14 2020-08-25 Inscripta, Inc. Instruments, modules, and methods for improved detection of edited sequences in live cells
US10532324B1 (en) 2018-08-14 2020-01-14 Inscripta, Inc. Instruments, modules, and methods for improved detection of edited sequences in live cells
WO2020081149A2 (en) 2018-08-30 2020-04-23 Inscripta, Inc. Improved detection of nuclease edited sequences in automated modules and instruments
KR102129681B1 (ko) * 2018-10-22 2020-07-02 부경대학교 산학협력단 연속공정식 액적 전기천공 장치 및 이를 이용한 연속공정식 액적 전기천공 방법
WO2020101254A1 (ko) * 2018-11-12 2020-05-22 주식회사 펨토바이오메드 세포 내 물질 전달을 제어하는 방법 및 장치
WO2020252215A1 (en) 2019-06-12 2020-12-17 Cellfe, Inc. Methods and systems for cell labeling and imaging
US10907125B2 (en) 2019-06-20 2021-02-02 Inscripta, Inc. Flow through electroporation modules and instrumentation
WO2020257395A1 (en) 2019-06-21 2020-12-24 Inscripta, Inc. Genome-wide rationally-designed mutations leading to enhanced lysine production in e. coli
US10927385B2 (en) 2019-06-25 2021-02-23 Inscripta, Inc. Increased nucleic-acid guided cell editing in yeast
US10689669B1 (en) 2020-01-11 2020-06-23 Inscripta, Inc. Automated multi-module cell processing methods, instruments, and systems
US11225674B2 (en) 2020-01-27 2022-01-18 Inscripta, Inc. Electroporation modules and instrumentation
JP7824650B2 (ja) * 2020-03-10 2026-03-05 ハインツテック株式会社 複合ナノチューブを介した細胞内物質導入および抽出技術
US20210332388A1 (en) 2020-04-24 2021-10-28 Inscripta, Inc. Compositions, methods, modules and instruments for automated nucleic acid-guided nuclease editing in mammalian cells
US11787841B2 (en) 2020-05-19 2023-10-17 Inscripta, Inc. Rationally-designed mutations to the thrA gene for enhanced lysine production in E. coli
CA3201944A1 (en) 2020-11-18 2022-05-27 Cellfe, Inc. Methods and systems for mechanoporation-based payload delivery into biological cells
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KR20160079659A (ko) 2016-07-06
KR20160079661A (ko) 2016-07-06
DK3037518T3 (da) 2020-07-13
KR102428464B1 (ko) 2022-08-03
JP2018500044A (ja) 2018-01-11
JP6883517B2 (ja) 2021-06-09
ES2808828T3 (es) 2021-03-02
JP2018500045A (ja) 2018-01-11
KR20160079660A (ko) 2016-07-06
JP6805152B2 (ja) 2020-12-23
JP2018504110A (ja) 2018-02-15
US20160272961A1 (en) 2016-09-22

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