DK3037518T3 - Indretning til at indsætte materiale i celle - Google Patents

Indretning til at indsætte materiale i celle Download PDF

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Publication number
DK3037518T3
DK3037518T3 DK15202528.4T DK15202528T DK3037518T3 DK 3037518 T3 DK3037518 T3 DK 3037518T3 DK 15202528 T DK15202528 T DK 15202528T DK 3037518 T3 DK3037518 T3 DK 3037518T3
Authority
DK
Denmark
Prior art keywords
cell
inserting material
Prior art date
Application number
DK15202528.4T
Other languages
Danish (da)
English (en)
Inventor
Sanghyun Lee
Original Assignee
Femtobiomed Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Femtobiomed Inc filed Critical Femtobiomed Inc
Application granted granted Critical
Publication of DK3037518T3 publication Critical patent/DK3037518T3/da

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N13/00—Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/02—Electrical or electromagnetic means, e.g. for electroporation or for cell fusion
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/04—Mechanical means, e.g. sonic waves, stretching forces, pressure or shear stimuli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Sustainable Development (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Electromagnetism (AREA)
  • Mechanical Engineering (AREA)
  • Developmental Biology & Embryology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Medicinal Chemistry (AREA)
  • Reproductive Health (AREA)
  • Analytical Chemistry (AREA)
  • Gynecology & Obstetrics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Clinical Laboratory Science (AREA)
  • Hematology (AREA)
  • Dispersion Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Transplantation (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
DK15202528.4T 2014-12-28 2015-12-23 Indretning til at indsætte materiale i celle DK3037518T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR20140191302 2014-12-28
KR1020150178130A KR102428463B1 (ko) 2014-12-28 2015-12-14 세포에 물질을 주입하는 장치 및 제조 방법

Publications (1)

Publication Number Publication Date
DK3037518T3 true DK3037518T3 (da) 2020-07-13

Family

ID=56502546

Family Applications (1)

Application Number Title Priority Date Filing Date
DK15202528.4T DK3037518T3 (da) 2014-12-28 2015-12-23 Indretning til at indsætte materiale i celle

Country Status (5)

Country Link
US (1) US20160272961A1 (enExample)
JP (3) JP6883517B2 (enExample)
KR (3) KR102428463B1 (enExample)
DK (1) DK3037518T3 (enExample)
ES (1) ES2808828T3 (enExample)

Families Citing this family (29)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3037518B1 (en) 2014-12-28 2020-05-06 Femtobiomed Inc. Device for putting material into cell
CN108251268A (zh) * 2016-12-29 2018-07-06 上海新微技术研发中心有限公司 一种基因转染器及基因转染方法
JP2020530979A (ja) 2017-06-30 2020-11-05 インスクリプタ， インコーポレイテッド 自動細胞処理方法、モジュール、機器及びシステム
US10738327B2 (en) 2017-08-28 2020-08-11 Inscripta, Inc. Electroporation cuvettes for automation
JP6985508B2 (ja) 2017-09-30 2021-12-22 インスクリプタ， インコーポレイテッド フロースルーエレクトロポレーション機器編成
US10443031B1 (en) 2018-03-29 2019-10-15 Inscripta, Inc. Methods for controlling the growth of prokaryotic and eukaryotic cells
WO2019200004A1 (en) 2018-04-13 2019-10-17 Inscripta, Inc. Automated cell processing instruments comprising reagent cartridges
US10557216B2 (en) 2018-04-24 2020-02-11 Inscripta, Inc. Automated instrumentation for production of T-cell receptor peptide libraries
WO2019209926A1 (en) 2018-04-24 2019-10-31 Inscripta, Inc. Automated instrumentation for production of peptide libraries
US10858761B2 (en) 2018-04-24 2020-12-08 Inscripta, Inc. Nucleic acid-guided editing of exogenous polynucleotides in heterologous cells
CA3108767A1 (en) 2018-06-30 2020-01-02 Inscripta, Inc. Instruments, modules, and methods for improved detection of edited sequences in live cells
US11142740B2 (en) 2018-08-14 2021-10-12 Inscripta, Inc. Detection of nuclease edited sequences in automated modules and instruments
US10752874B2 (en) 2018-08-14 2020-08-25 Inscripta, Inc. Instruments, modules, and methods for improved detection of edited sequences in live cells
US10532324B1 (en) 2018-08-14 2020-01-14 Inscripta, Inc. Instruments, modules, and methods for improved detection of edited sequences in live cells
WO2020081149A2 (en) 2018-08-30 2020-04-23 Inscripta, Inc. Improved detection of nuclease edited sequences in automated modules and instruments
KR102129681B1 (ko) * 2018-10-22 2020-07-02 부경대학교 산학협력단 연속공정식 액적 전기천공 장치 및 이를 이용한 연속공정식 액적 전기천공 방법
WO2020101254A1 (ko) * 2018-11-12 2020-05-22 주식회사 펨토바이오메드 세포 내 물질 전달을 제어하는 방법 및 장치
WO2020252215A1 (en) 2019-06-12 2020-12-17 Cellfe, Inc. Methods and systems for cell labeling and imaging
US10907125B2 (en) 2019-06-20 2021-02-02 Inscripta, Inc. Flow through electroporation modules and instrumentation
WO2020257395A1 (en) 2019-06-21 2020-12-24 Inscripta, Inc. Genome-wide rationally-designed mutations leading to enhanced lysine production in e. coli
US10927385B2 (en) 2019-06-25 2021-02-23 Inscripta, Inc. Increased nucleic-acid guided cell editing in yeast
US10689669B1 (en) 2020-01-11 2020-06-23 Inscripta, Inc. Automated multi-module cell processing methods, instruments, and systems
US11225674B2 (en) 2020-01-27 2022-01-18 Inscripta, Inc. Electroporation modules and instrumentation
JP7824650B2 (ja) * 2020-03-10 2026-03-05 ハインツテック株式会社 複合ナノチューブを介した細胞内物質導入および抽出技術
US20210332388A1 (en) 2020-04-24 2021-10-28 Inscripta, Inc. Compositions, methods, modules and instruments for automated nucleic acid-guided nuclease editing in mammalian cells
US11787841B2 (en) 2020-05-19 2023-10-17 Inscripta, Inc. Rationally-designed mutations to the thrA gene for enhanced lysine production in E. coli
CA3201944A1 (en) 2020-11-18 2022-05-27 Cellfe, Inc. Methods and systems for mechanoporation-based payload delivery into biological cells
US12227729B2 (en) 2020-12-24 2025-02-18 Cellfe, Inc. Methods and systems for high-throughput cell processing
GB202206443D0 (en) * 2022-05-03 2022-06-15 Ttp Plc Apparatus and method

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH08322548A (ja) * 1995-05-31 1996-12-10 Nikon Corp 細胞操作方法および細胞操作装置
US6027488A (en) * 1998-06-03 2000-02-22 Genetronics, Inc. Flow-through electroporation system for ex vivo gene therapy
SE0003841D0 (sv) * 2000-10-20 2000-10-20 Daniel Chiu A method and apparatus for penetration of lipid bilayer membranes
CA2468424A1 (en) * 2001-11-27 2003-06-05 Cecilia Farre A method for combined parallel agent delivery and electroporation for cell structures and use thereof
JP4530991B2 (ja) * 2003-04-11 2010-08-25 独立行政法人理化学研究所 マイクロインジェクション方法および装置
US7338796B1 (en) * 2003-08-13 2008-03-04 Sandia Corporation Vesicle-based method and apparatus for collecting, manipulating, and chemically processing trace macromolecular species
US20050170510A1 (en) * 2003-12-08 2005-08-04 Yong Huang Device and method for controlled electroporation and molecular delivery into cells and tissue
JP2005278480A (ja) * 2004-03-29 2005-10-13 Fujitsu Ltd 物質導入装置及び物質導入用チップ
US20080248575A1 (en) * 2005-10-20 2008-10-09 The Ohio State University Research Foundation Drug and Gene Delivery by Polymer Nanonozzle and Nanotip Cell Patch
JP4713671B1 (ja) * 2010-01-22 2011-06-29 ネッパジーン株式会社 エレクトロポレーション法による外来遺伝子導入法
JP5585204B2 (ja) * 2010-05-18 2014-09-10 大日本印刷株式会社 マイクロチップ及びその製造方法
US9816086B2 (en) * 2010-07-06 2017-11-14 The Ohio State University Dose and location controlled drug/gene/particle delivery to individual cells by nanoelectroporation
CN101928666B (zh) * 2010-07-30 2013-04-03 北京大学 一种流式电穿孔装置及系统
PT2768942T (pt) * 2011-10-17 2020-01-21 Massachusetts Inst Technology Entrega intracelular

Also Published As

Publication number Publication date
KR20160079659A (ko) 2016-07-06
KR20160079661A (ko) 2016-07-06
KR102428464B1 (ko) 2022-08-03
KR102428463B1 (ko) 2022-08-03
JP2018500044A (ja) 2018-01-11
JP6883517B2 (ja) 2021-06-09
ES2808828T3 (es) 2021-03-02
JP2018500045A (ja) 2018-01-11
KR20160079660A (ko) 2016-07-06
JP6805152B2 (ja) 2020-12-23
JP2018504110A (ja) 2018-02-15
US20160272961A1 (en) 2016-09-22

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