KR20160079661A - 운반체의 사용 없이 세포에 물질을 주입하여 변환시킨 세포 - Google Patents

운반체의 사용 없이 세포에 물질을 주입하여 변환시킨 세포 Download PDF

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KR20160079661A
KR20160079661A KR1020150178234A KR20150178234A KR20160079661A KR 20160079661 A KR20160079661 A KR 20160079661A KR 1020150178234 A KR1020150178234 A KR 1020150178234A KR 20150178234 A KR20150178234 A KR 20150178234A KR 20160079661 A KR20160079661 A KR 20160079661A
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cell
cells
passage
transformed
passageway
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Korean (ko)
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이상현
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주식회사 펨토펩
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Priority to EP15202502.9A priority Critical patent/EP3037515A1/en
Priority to PCT/KR2015/014295 priority patent/WO2016108529A1/en
Priority to JP2017535708A priority patent/JP2018504110A/ja
Priority to US14/980,766 priority patent/US20160186212A1/en
Publication of KR20160079661A publication Critical patent/KR20160079661A/ko
Ceased legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
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    • C12N13/00—Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502707—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the manufacture of the container or its components
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502715—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
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    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/42—Apparatus for the treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/02—Electrical or electromagnetic means, e.g. for electroporation or for cell fusion
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/04—Mechanical means, e.g. sonic waves, stretching forces, pressure or shear stimuli
    • C—CHEMISTRY; METALLURGY
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    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0608—Germ cells
    • C12N5/0609—Oocytes, oogonia
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0608—Germ cells
    • C12N5/061—Sperm cells, spermatogonia
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0696—Artificially induced pluripotent stem cells, e.g. iPS
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0896—Nanoscaled

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  • Apparatus Associated With Microorganisms And Enzymes (AREA)
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  • Transplantation (AREA)
  • Tropical Medicine & Parasitology (AREA)
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KR1020150178234A 2014-12-28 2015-12-14 운반체의 사용 없이 세포에 물질을 주입하여 변환시킨 세포 Ceased KR20160079661A (ko)

Priority Applications (4)

Application Number Priority Date Filing Date Title
EP15202502.9A EP3037515A1 (en) 2014-12-28 2015-12-23 Modified cell prepared by putting material into the cell without using delivery vehicle
PCT/KR2015/014295 WO2016108529A1 (en) 2014-12-28 2015-12-24 Modified cell prepared by putting material into the cell without using delivery vehicle
JP2017535708A JP2018504110A (ja) 2014-12-28 2015-12-24 運搬体の使用なしで細胞に物質を注入して変換させた細胞（Ｍｏｄｉｆｉｅｄ Ｃｅｌｌ ｂｙ Ｐｕｔｔｉｎｇ Ｍａｔｅｒｉａｌ ｉｎｔｏ ｔｈｅ Ｃｅｌｌ ｗｉｔｈｏｕｔ Ｕｓｉｎｇ Ｖｅｈｉｃｌｅ）［発明の詳細な説明］
US14/980,766 US20160186212A1 (en) 2014-12-28 2015-12-28 Modified cell by putting material into the cell without using delivery vehicle

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR20140191302 2014-12-28
KR1020140191302 2014-12-28

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KR20160079661A true KR20160079661A (ko) 2016-07-06

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KR1020150178234A Ceased KR20160079661A (ko) 2014-12-28 2015-12-14 운반체의 사용 없이 세포에 물질을 주입하여 변환시킨 세포
KR1020150178183A Active KR102428464B1 (ko) 2014-12-28 2015-12-14 세포에 물질을 주입하여 세포를 변환시키는 방법
KR1020150178130A Active KR102428463B1 (ko) 2014-12-28 2015-12-14 세포에 물질을 주입하는 장치 및 제조 방법

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KR1020150178130A Active KR102428463B1 (ko) 2014-12-28 2015-12-14 세포에 물질을 주입하는 장치 및 제조 방법

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US (1) US20160272961A1 (enExample)
JP (3) JP6883517B2 (enExample)
KR (3) KR20160079661A (enExample)
DK (1) DK3037518T3 (enExample)
ES (1) ES2808828T3 (enExample)

Families Citing this family (29)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3037518B1 (en) 2014-12-28 2020-05-06 Femtobiomed Inc. Device for putting material into cell
CN108251268A (zh) * 2016-12-29 2018-07-06 上海新微技术研发中心有限公司 一种基因转染器及基因转染方法
HUE058668T2 (hu) 2017-06-30 2022-09-28 Inscripta Inc Automatizált sejtkezelési eljárások, modulok, berendezések és rendszerek
US10738327B2 (en) 2017-08-28 2020-08-11 Inscripta, Inc. Electroporation cuvettes for automation
WO2019068022A1 (en) * 2017-09-30 2019-04-04 Inscripta, Inc. CONTINUOUS FLOW ELECTROPORATION INSTRUMENTATION
WO2019190874A1 (en) 2018-03-29 2019-10-03 Inscripta, Inc. Automated control of cell growth rates for induction and transformation
WO2019200004A1 (en) 2018-04-13 2019-10-17 Inscripta, Inc. Automated cell processing instruments comprising reagent cartridges
US10526598B2 (en) 2018-04-24 2020-01-07 Inscripta, Inc. Methods for identifying T-cell receptor antigens
US10508273B2 (en) 2018-04-24 2019-12-17 Inscripta, Inc. Methods for identifying selective binding pairs
US10858761B2 (en) 2018-04-24 2020-12-08 Inscripta, Inc. Nucleic acid-guided editing of exogenous polynucleotides in heterologous cells
AU2019292919A1 (en) 2018-06-30 2021-03-11 Inscripta, Inc. Instruments, modules, and methods for improved detection of edited sequences in live cells
US10532324B1 (en) 2018-08-14 2020-01-14 Inscripta, Inc. Instruments, modules, and methods for improved detection of edited sequences in live cells
US10752874B2 (en) 2018-08-14 2020-08-25 Inscripta, Inc. Instruments, modules, and methods for improved detection of edited sequences in live cells
US11142740B2 (en) 2018-08-14 2021-10-12 Inscripta, Inc. Detection of nuclease edited sequences in automated modules and instruments
US11965154B2 (en) 2018-08-30 2024-04-23 Inscripta, Inc. Detection of nuclease edited sequences in automated modules and instruments
KR102129681B1 (ko) 2018-10-22 2020-07-02 부경대학교 산학협력단 연속공정식 액적 전기천공 장치 및 이를 이용한 연속공정식 액적 전기천공 방법
CN112840015A (zh) * 2018-11-12 2021-05-25 飞秒生物医学有限公司 控制细胞内递送物质的方法及装置
WO2020252215A1 (en) 2019-06-12 2020-12-17 Cellfe, Inc. Methods and systems for cell labeling and imaging
US10907125B2 (en) 2019-06-20 2021-02-02 Inscripta, Inc. Flow through electroporation modules and instrumentation
WO2020257395A1 (en) 2019-06-21 2020-12-24 Inscripta, Inc. Genome-wide rationally-designed mutations leading to enhanced lysine production in e. coli
US10927385B2 (en) 2019-06-25 2021-02-23 Inscripta, Inc. Increased nucleic-acid guided cell editing in yeast
US10689669B1 (en) 2020-01-11 2020-06-23 Inscripta, Inc. Automated multi-module cell processing methods, instruments, and systems
KR20220133257A (ko) 2020-01-27 2022-10-04 인스크립타 인코포레이티드 전기천공 모듈 및 기구
CN115605572A (zh) * 2020-03-10 2023-01-13 哈因斯科技株式会社（Jp） 经由复合纳米管的细胞内物质导入和提取技术
US20210332388A1 (en) 2020-04-24 2021-10-28 Inscripta, Inc. Compositions, methods, modules and instruments for automated nucleic acid-guided nuclease editing in mammalian cells
US11787841B2 (en) 2020-05-19 2023-10-17 Inscripta, Inc. Rationally-designed mutations to the thrA gene for enhanced lysine production in E. coli
CA3201944A1 (en) 2020-11-18 2022-05-27 Cellfe, Inc. Methods and systems for mechanoporation-based payload delivery into biological cells
US12227729B2 (en) 2020-12-24 2025-02-18 Cellfe, Inc. Methods and systems for high-throughput cell processing
GB202206443D0 (en) * 2022-05-03 2022-06-15 Ttp Plc Apparatus and method

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH08322548A (ja) * 1995-05-31 1996-12-10 Nikon Corp 細胞操作方法および細胞操作装置
US6027488A (en) 1998-06-03 2000-02-22 Genetronics, Inc. Flow-through electroporation system for ex vivo gene therapy
SE0003841D0 (sv) * 2000-10-20 2000-10-20 Daniel Chiu A method and apparatus for penetration of lipid bilayer membranes
AU2002353741A1 (en) 2001-11-27 2003-06-10 Cellectricon Ab A method for combined sequential agent delivery and electroporation for cell structures and use thereof
JP4530991B2 (ja) * 2003-04-11 2010-08-25 独立行政法人理化学研究所 マイクロインジェクション方法および装置
US7338796B1 (en) * 2003-08-13 2008-03-04 Sandia Corporation Vesicle-based method and apparatus for collecting, manipulating, and chemically processing trace macromolecular species
WO2005056788A1 (en) * 2003-12-08 2005-06-23 Excellin Life Sciences, Inc. Device and method for controlled electroporation and molecular delivery into cells and tissue
JP2005278480A (ja) * 2004-03-29 2005-10-13 Fujitsu Ltd 物質導入装置及び物質導入用チップ
US20080248575A1 (en) * 2005-10-20 2008-10-09 The Ohio State University Research Foundation Drug and Gene Delivery by Polymer Nanonozzle and Nanotip Cell Patch
JP4713671B1 (ja) * 2010-01-22 2011-06-29 ネッパジーン株式会社 エレクトロポレーション法による外来遺伝子導入法
JP5585204B2 (ja) * 2010-05-18 2014-09-10 大日本印刷株式会社 マイクロチップ及びその製造方法
US9816086B2 (en) * 2010-07-06 2017-11-14 The Ohio State University Dose and location controlled drug/gene/particle delivery to individual cells by nanoelectroporation
CN101928666B (zh) * 2010-07-30 2013-04-03 北京大学 一种流式电穿孔装置及系统
ES2764105T3 (es) * 2011-10-17 2020-06-02 Massachusetts Inst Technology Administración intracelular

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JP6805152B2 (ja) 2020-12-23
ES2808828T3 (es) 2021-03-02
US20160272961A1 (en) 2016-09-22
JP2018504110A (ja) 2018-02-15
KR20160079659A (ko) 2016-07-06
JP2018500044A (ja) 2018-01-11
KR102428464B1 (ko) 2022-08-03
KR102428463B1 (ko) 2022-08-03
JP6883517B2 (ja) 2021-06-09
JP2018500045A (ja) 2018-01-11
KR20160079660A (ko) 2016-07-06
DK3037518T3 (da) 2020-07-13

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