JP6883517B2 - 細胞に物質を注入する装置および製造方法（Ｄｅｖｉｃｅ ｆｏｒ Ｐｕｔｔｉｎｇ Ｍａｔｅｒｉａｌ ｉｎｔｏ Ｃｅｌｌ） - Google Patents

細胞に物質を注入する装置および製造方法（Ｄｅｖｉｃｅ ｆｏｒ Ｐｕｔｔｉｎｇ Ｍａｔｅｒｉａｌ ｉｎｔｏ Ｃｅｌｌ） Download PDF

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JP6883517B2
JP6883517B2 JP2017535710A JP2017535710A JP6883517B2 JP 6883517 B2 JP6883517 B2 JP 6883517B2 JP 2017535710 A JP2017535710 A JP 2017535710A JP 2017535710 A JP2017535710 A JP 2017535710A JP 6883517 B2 JP6883517 B2 JP 6883517B2
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passage
cells
substance
cell
injecting
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JP2018500044A5 (enExample
JP2018500044A (ja
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サンギュン リー
サンギュン リー
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Femtobiomed Inc
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N13/00—Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/02—Electrical or electromagnetic means, e.g. for electroporation or for cell fusion
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/04—Mechanical means, e.g. sonic waves, stretching forces, pressure or shear stimuli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation

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  • Electromagnetism (AREA)
  • Mechanical Engineering (AREA)
  • Developmental Biology & Embryology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
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  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
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JP2017535710A 2014-12-28 2015-12-24 細胞に物質を注入する装置および製造方法（Ｄｅｖｉｃｅ ｆｏｒ Ｐｕｔｔｉｎｇ Ｍａｔｅｒｉａｌ ｉｎｔｏ Ｃｅｌｌ） Active JP6883517B2 (ja)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
KR10-2014-0191302 2014-12-28
KR20140191302 2014-12-28
KR10-2015-0178130 2015-12-14
KR1020150178130A KR102428463B1 (ko) 2014-12-28 2015-12-14 세포에 물질을 주입하는 장치 및 제조 방법
PCT/KR2015/014292 WO2016108526A1 (en) 2014-12-28 2015-12-24 Device for putting material into cell

Publications (3)

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JP2018500044A JP2018500044A (ja) 2018-01-11
JP2018500044A5 JP2018500044A5 (enExample) 2019-02-07
JP6883517B2 true JP6883517B2 (ja) 2021-06-09

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JP2017535710A Active JP6883517B2 (ja) 2014-12-28 2015-12-24 細胞に物質を注入する装置および製造方法（Ｄｅｖｉｃｅ ｆｏｒ Ｐｕｔｔｉｎｇ Ｍａｔｅｒｉａｌ ｉｎｔｏ Ｃｅｌｌ）
JP2017535711A Active JP6805152B2 (ja) 2014-12-28 2015-12-24 細胞に物質を注入して細胞を変換させる方法｛Ｍｅｔｈｏｄ ｆｏｒ Ｍｏｄｉｆｙｉｎｇ ａ Ｃｅｌｌ ｂｙ Ｐｕｔｔｉｎｇ Ｍａｔｅｒｉａｌ ｉｎｔｏ ｔｈｅ Ｃｅｌｌ｝
JP2017535708A Pending JP2018504110A (ja) 2014-12-28 2015-12-24 運搬体の使用なしで細胞に物質を注入して変換させた細胞（Ｍｏｄｉｆｉｅｄ Ｃｅｌｌ ｂｙ Ｐｕｔｔｉｎｇ Ｍａｔｅｒｉａｌ ｉｎｔｏ ｔｈｅ Ｃｅｌｌ ｗｉｔｈｏｕｔ Ｕｓｉｎｇ Ｖｅｈｉｃｌｅ）［発明の詳細な説明］

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JP2017535711A Active JP6805152B2 (ja) 2014-12-28 2015-12-24 細胞に物質を注入して細胞を変換させる方法｛Ｍｅｔｈｏｄ ｆｏｒ Ｍｏｄｉｆｙｉｎｇ ａ Ｃｅｌｌ ｂｙ Ｐｕｔｔｉｎｇ Ｍａｔｅｒｉａｌ ｉｎｔｏ ｔｈｅ Ｃｅｌｌ｝
JP2017535708A Pending JP2018504110A (ja) 2014-12-28 2015-12-24 運搬体の使用なしで細胞に物質を注入して変換させた細胞（Ｍｏｄｉｆｉｅｄ Ｃｅｌｌ ｂｙ Ｐｕｔｔｉｎｇ Ｍａｔｅｒｉａｌ ｉｎｔｏ ｔｈｅ Ｃｅｌｌ ｗｉｔｈｏｕｔ Ｕｓｉｎｇ Ｖｅｈｉｃｌｅ）［発明の詳細な説明］

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Country Link
US (1) US20160272961A1 (enExample)
JP (3) JP6883517B2 (enExample)
KR (3) KR102428463B1 (enExample)
DK (1) DK3037518T3 (enExample)
ES (1) ES2808828T3 (enExample)

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EP3037518B1 (en) 2014-12-28 2020-05-06 Femtobiomed Inc. Device for putting material into cell
CN108251268A (zh) * 2016-12-29 2018-07-06 上海新微技术研发中心有限公司 一种基因转染器及基因转染方法
JP2020530979A (ja) 2017-06-30 2020-11-05 インスクリプタ， インコーポレイテッド 自動細胞処理方法、モジュール、機器及びシステム
US10738327B2 (en) 2017-08-28 2020-08-11 Inscripta, Inc. Electroporation cuvettes for automation
JP6985508B2 (ja) 2017-09-30 2021-12-22 インスクリプタ， インコーポレイテッド フロースルーエレクトロポレーション機器編成
US10443031B1 (en) 2018-03-29 2019-10-15 Inscripta, Inc. Methods for controlling the growth of prokaryotic and eukaryotic cells
WO2019200004A1 (en) 2018-04-13 2019-10-17 Inscripta, Inc. Automated cell processing instruments comprising reagent cartridges
US10557216B2 (en) 2018-04-24 2020-02-11 Inscripta, Inc. Automated instrumentation for production of T-cell receptor peptide libraries
WO2019209926A1 (en) 2018-04-24 2019-10-31 Inscripta, Inc. Automated instrumentation for production of peptide libraries
US10858761B2 (en) 2018-04-24 2020-12-08 Inscripta, Inc. Nucleic acid-guided editing of exogenous polynucleotides in heterologous cells
CA3108767A1 (en) 2018-06-30 2020-01-02 Inscripta, Inc. Instruments, modules, and methods for improved detection of edited sequences in live cells
US11142740B2 (en) 2018-08-14 2021-10-12 Inscripta, Inc. Detection of nuclease edited sequences in automated modules and instruments
US10752874B2 (en) 2018-08-14 2020-08-25 Inscripta, Inc. Instruments, modules, and methods for improved detection of edited sequences in live cells
US10532324B1 (en) 2018-08-14 2020-01-14 Inscripta, Inc. Instruments, modules, and methods for improved detection of edited sequences in live cells
WO2020081149A2 (en) 2018-08-30 2020-04-23 Inscripta, Inc. Improved detection of nuclease edited sequences in automated modules and instruments
KR102129681B1 (ko) * 2018-10-22 2020-07-02 부경대학교 산학협력단 연속공정식 액적 전기천공 장치 및 이를 이용한 연속공정식 액적 전기천공 방법
WO2020101254A1 (ko) * 2018-11-12 2020-05-22 주식회사 펨토바이오메드 세포 내 물질 전달을 제어하는 방법 및 장치
WO2020252215A1 (en) 2019-06-12 2020-12-17 Cellfe, Inc. Methods and systems for cell labeling and imaging
US10907125B2 (en) 2019-06-20 2021-02-02 Inscripta, Inc. Flow through electroporation modules and instrumentation
WO2020257395A1 (en) 2019-06-21 2020-12-24 Inscripta, Inc. Genome-wide rationally-designed mutations leading to enhanced lysine production in e. coli
US10927385B2 (en) 2019-06-25 2021-02-23 Inscripta, Inc. Increased nucleic-acid guided cell editing in yeast
US10689669B1 (en) 2020-01-11 2020-06-23 Inscripta, Inc. Automated multi-module cell processing methods, instruments, and systems
US11225674B2 (en) 2020-01-27 2022-01-18 Inscripta, Inc. Electroporation modules and instrumentation
JP7824650B2 (ja) * 2020-03-10 2026-03-05 ハインツテック株式会社 複合ナノチューブを介した細胞内物質導入および抽出技術
US20210332388A1 (en) 2020-04-24 2021-10-28 Inscripta, Inc. Compositions, methods, modules and instruments for automated nucleic acid-guided nuclease editing in mammalian cells
US11787841B2 (en) 2020-05-19 2023-10-17 Inscripta, Inc. Rationally-designed mutations to the thrA gene for enhanced lysine production in E. coli
CA3201944A1 (en) 2020-11-18 2022-05-27 Cellfe, Inc. Methods and systems for mechanoporation-based payload delivery into biological cells
US12227729B2 (en) 2020-12-24 2025-02-18 Cellfe, Inc. Methods and systems for high-throughput cell processing
GB202206443D0 (en) * 2022-05-03 2022-06-15 Ttp Plc Apparatus and method

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JPH08322548A (ja) * 1995-05-31 1996-12-10 Nikon Corp 細胞操作方法および細胞操作装置
US6027488A (en) * 1998-06-03 2000-02-22 Genetronics, Inc. Flow-through electroporation system for ex vivo gene therapy
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JP4530991B2 (ja) * 2003-04-11 2010-08-25 独立行政法人理化学研究所 マイクロインジェクション方法および装置
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KR20160079659A (ko) 2016-07-06
KR20160079661A (ko) 2016-07-06
DK3037518T3 (da) 2020-07-13
KR102428464B1 (ko) 2022-08-03
KR102428463B1 (ko) 2022-08-03
JP2018500044A (ja) 2018-01-11
ES2808828T3 (es) 2021-03-02
JP2018500045A (ja) 2018-01-11
KR20160079660A (ko) 2016-07-06
JP6805152B2 (ja) 2020-12-23
JP2018504110A (ja) 2018-02-15
US20160272961A1 (en) 2016-09-22

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