KR102406585B1 - 게놈 dna를 변형하기 위한 방법 및 조성물 - Google Patents
게놈 dna를 변형하기 위한 방법 및 조성물 Download PDFInfo
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- KR102406585B1 KR102406585B1 KR1020167031636A KR20167031636A KR102406585B1 KR 102406585 B1 KR102406585 B1 KR 102406585B1 KR 1020167031636 A KR1020167031636 A KR 1020167031636A KR 20167031636 A KR20167031636 A KR 20167031636A KR 102406585 B1 KR102406585 B1 KR 102406585B1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/102—Mutagenizing nucleic acids
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P29/00—Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P35/00—Antineoplastic agents
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P7/00—Drugs for disorders of the blood or the extracellular fluid
- A61P7/06—Antianaemics
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
- C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
- C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0634—Cells from the blood or the immune system
- C12N5/0647—Haematopoietic stem cells; Uncommitted or multipotent progenitors
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2800/00—Nucleic acids vectors
- C12N2800/80—Vectors containing sites for inducing double-stranded breaks, e.g. meganuclease restriction sites
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- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
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- Biomedical Technology (AREA)
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- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Medicinal Chemistry (AREA)
- Public Health (AREA)
- Animal Behavior & Ethology (AREA)
- Veterinary Medicine (AREA)
- Pharmacology & Pharmacy (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Cell Biology (AREA)
- Hematology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Mycology (AREA)
- Developmental Biology & Embryology (AREA)
- Epidemiology (AREA)
- Immunology (AREA)
- Communicable Diseases (AREA)
- Oncology (AREA)
- Diabetes (AREA)
- Pain & Pain Management (AREA)
- Rheumatology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201461979178P | 2014-04-14 | 2014-04-14 | |
| US61/979,178 | 2014-04-14 | ||
| US201462078706P | 2014-11-12 | 2014-11-12 | |
| US62/078,706 | 2014-11-12 | ||
| PCT/US2015/025523 WO2015160683A1 (en) | 2014-04-14 | 2015-04-13 | Methods and compositions for modifying genomic dna |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| KR20170026332A KR20170026332A (ko) | 2017-03-08 |
| KR102406585B1 true KR102406585B1 (ko) | 2022-06-07 |
Family
ID=54324457
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| KR1020167031636A Active KR102406585B1 (ko) | 2014-04-14 | 2015-04-13 | 게놈 dna를 변형하기 위한 방법 및 조성물 |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US20170029805A1 (https=) |
| EP (1) | EP3132025B1 (https=) |
| JP (2) | JP2017513477A (https=) |
| KR (1) | KR102406585B1 (https=) |
| CN (1) | CN106687585B (https=) |
| ES (1) | ES2962509T3 (https=) |
| SG (1) | SG11201608503UA (https=) |
| WO (1) | WO2015160683A1 (https=) |
Families Citing this family (79)
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| JP6683598B2 (ja) | 2013-03-15 | 2020-04-22 | マックスサイト インコーポレーティッド | 安定なトランスフェクトされた細胞を生成するための方法および組成物 |
| US9163284B2 (en) | 2013-08-09 | 2015-10-20 | President And Fellows Of Harvard College | Methods for identifying a target site of a Cas9 nuclease |
| US9359599B2 (en) | 2013-08-22 | 2016-06-07 | President And Fellows Of Harvard College | Engineered transcription activator-like effector (TALE) domains and uses thereof |
| US9322037B2 (en) | 2013-09-06 | 2016-04-26 | President And Fellows Of Harvard College | Cas9-FokI fusion proteins and uses thereof |
| US9526784B2 (en) | 2013-09-06 | 2016-12-27 | President And Fellows Of Harvard College | Delivery system for functional nucleases |
| US9228207B2 (en) | 2013-09-06 | 2016-01-05 | President And Fellows Of Harvard College | Switchable gRNAs comprising aptamers |
| WO2015066119A1 (en) | 2013-10-30 | 2015-05-07 | North Carolina State University | Compositions and methods related to a type-ii crispr-cas system in lactobacillus buchneri |
| CN106459995B (zh) | 2013-11-07 | 2020-02-21 | 爱迪塔斯医药有限公司 | 使用统治型gRNA的CRISPR相关方法和组合物 |
| US20150165054A1 (en) | 2013-12-12 | 2015-06-18 | President And Fellows Of Harvard College | Methods for correcting caspase-9 point mutations |
| JP2017512481A (ja) | 2014-04-08 | 2017-05-25 | ノースカロライナ ステート ユニバーシティーNorth Carolina State University | Crispr関連遺伝子を用いた、rna依存性の転写抑制のための方法および組成物 |
| GB201407852D0 (en) | 2014-05-02 | 2014-06-18 | Iontas Ltd | Preparation of libraries od protein variants expressed in eukaryotic cells and use for selecting binding molecules |
| US10077453B2 (en) | 2014-07-30 | 2018-09-18 | President And Fellows Of Harvard College | CAS9 proteins including ligand-dependent inteins |
| EP3186375A4 (en) | 2014-08-28 | 2019-03-13 | North Carolina State University | Novel CAS9 PROTEINS AND CHARACTERISTICS FOR DNA TARGETING AND GENOME EDITING |
| AU2015330699B2 (en) | 2014-10-10 | 2021-12-02 | Editas Medicine, Inc. | Compositions and methods for promoting homology directed repair |
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| KR20190031306A (ko) * | 2016-07-21 | 2019-03-25 | 맥스시티 인코포레이티드 | 게놈 dna를 변경하기 위한 방법 및 조성물 |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20030032175A1 (en) | 2000-09-15 | 2003-02-13 | Christian Siebel | Methods of producing cells and animals comprising targeted gene modifications and compositions relating thereto |
| WO2007118208A2 (en) * | 2006-04-10 | 2007-10-18 | The Regents Of The University Of California | Systems for collection of single cells and colonies |
| WO2012012738A1 (en) * | 2010-07-23 | 2012-01-26 | Sigma-Aldrich Co., Llc | Genome editing using targeting endonucleases and single-stranded nucleic acids |
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| JP6683598B2 (ja) * | 2013-03-15 | 2020-04-22 | マックスサイト インコーポレーティッド | 安定なトランスフェクトされた細胞を生成するための方法および組成物 |
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-
2015
- 2015-04-13 EP EP15780038.4A patent/EP3132025B1/en active Active
- 2015-04-13 SG SG11201608503UA patent/SG11201608503UA/en unknown
- 2015-04-13 JP JP2016562503A patent/JP2017513477A/ja active Pending
- 2015-04-13 US US15/303,932 patent/US20170029805A1/en not_active Abandoned
- 2015-04-13 WO PCT/US2015/025523 patent/WO2015160683A1/en not_active Ceased
- 2015-04-13 KR KR1020167031636A patent/KR102406585B1/ko active Active
- 2015-04-13 CN CN201580025136.0A patent/CN106687585B/zh active Active
- 2015-04-13 ES ES15780038T patent/ES2962509T3/es active Active
-
2020
- 2020-07-29 JP JP2020127917A patent/JP7209671B2/ja active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20030032175A1 (en) | 2000-09-15 | 2003-02-13 | Christian Siebel | Methods of producing cells and animals comprising targeted gene modifications and compositions relating thereto |
| WO2007118208A2 (en) * | 2006-04-10 | 2007-10-18 | The Regents Of The University Of California | Systems for collection of single cells and colonies |
| WO2012012738A1 (en) * | 2010-07-23 | 2012-01-26 | Sigma-Aldrich Co., Llc | Genome editing using targeting endonucleases and single-stranded nucleic acids |
Also Published As
| Publication number | Publication date |
|---|---|
| EP3132025A1 (en) | 2017-02-22 |
| EP3132025A4 (en) | 2017-10-18 |
| WO2015160683A1 (en) | 2015-10-22 |
| SG11201608503UA (en) | 2016-11-29 |
| KR20170026332A (ko) | 2017-03-08 |
| JP2017513477A (ja) | 2017-06-01 |
| JP2021010366A (ja) | 2021-02-04 |
| CN106687585A (zh) | 2017-05-17 |
| CN106687585B (zh) | 2021-11-02 |
| JP7209671B2 (ja) | 2023-01-20 |
| EP3132025B1 (en) | 2023-08-30 |
| US20170029805A1 (en) | 2017-02-02 |
| ES2962509T3 (es) | 2024-03-19 |
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