JP2017513477A - ゲノムdnaを改変するための方法および組成物 - Google Patents
ゲノムdnaを改変するための方法および組成物 Download PDFInfo
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- JP2017513477A JP2017513477A JP2016562503A JP2016562503A JP2017513477A JP 2017513477 A JP2017513477 A JP 2017513477A JP 2016562503 A JP2016562503 A JP 2016562503A JP 2016562503 A JP2016562503 A JP 2016562503A JP 2017513477 A JP2017513477 A JP 2017513477A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/102—Mutagenizing nucleic acids
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P29/00—Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P35/00—Antineoplastic agents
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P7/00—Drugs for disorders of the blood or the extracellular fluid
- A61P7/06—Antianaemics
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
- C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
- C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0634—Cells from the blood or the immune system
- C12N5/0647—Haematopoietic stem cells; Uncommitted or multipotent progenitors
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2800/00—Nucleic acids vectors
- C12N2800/80—Vectors containing sites for inducing double-stranded breaks, e.g. meganuclease restriction sites
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Medicinal Chemistry (AREA)
- Public Health (AREA)
- Animal Behavior & Ethology (AREA)
- Veterinary Medicine (AREA)
- Pharmacology & Pharmacy (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- General Chemical & Material Sciences (AREA)
- Cell Biology (AREA)
- Hematology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Mycology (AREA)
- Developmental Biology & Embryology (AREA)
- Epidemiology (AREA)
- Immunology (AREA)
- Diabetes (AREA)
- Pain & Pain Management (AREA)
- Oncology (AREA)
- Rheumatology (AREA)
- Communicable Diseases (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201461979178P | 2014-04-14 | 2014-04-14 | |
| US61/979,178 | 2014-04-14 | ||
| US201462078706P | 2014-11-12 | 2014-11-12 | |
| US62/078,706 | 2014-11-12 | ||
| PCT/US2015/025523 WO2015160683A1 (en) | 2014-04-14 | 2015-04-13 | Methods and compositions for modifying genomic dna |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2020127917A Division JP7209671B2 (ja) | 2014-04-14 | 2020-07-29 | ゲノムdnaを改変するための方法および組成物 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2017513477A true JP2017513477A (ja) | 2017-06-01 |
| JP2017513477A5 JP2017513477A5 (https=) | 2018-05-24 |
Family
ID=54324457
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2016562503A Pending JP2017513477A (ja) | 2014-04-14 | 2015-04-13 | ゲノムdnaを改変するための方法および組成物 |
| JP2020127917A Active JP7209671B2 (ja) | 2014-04-14 | 2020-07-29 | ゲノムdnaを改変するための方法および組成物 |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2020127917A Active JP7209671B2 (ja) | 2014-04-14 | 2020-07-29 | ゲノムdnaを改変するための方法および組成物 |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US20170029805A1 (https=) |
| EP (1) | EP3132025B1 (https=) |
| JP (2) | JP2017513477A (https=) |
| KR (1) | KR102406585B1 (https=) |
| CN (1) | CN106687585B (https=) |
| ES (1) | ES2962509T3 (https=) |
| SG (1) | SG11201608503UA (https=) |
| WO (1) | WO2015160683A1 (https=) |
Families Citing this family (79)
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| JP6261500B2 (ja) | 2011-07-22 | 2018-01-17 | プレジデント アンド フェローズ オブ ハーバード カレッジ | ヌクレアーゼ切断特異性の評価および改善 |
| CN105378071B (zh) | 2013-03-15 | 2021-04-20 | 美克斯细胞有限公司 | 用于产生稳定转染细胞的方法和组合物 |
| US20150044192A1 (en) | 2013-08-09 | 2015-02-12 | President And Fellows Of Harvard College | Methods for identifying a target site of a cas9 nuclease |
| US9359599B2 (en) | 2013-08-22 | 2016-06-07 | President And Fellows Of Harvard College | Engineered transcription activator-like effector (TALE) domains and uses thereof |
| US9388430B2 (en) | 2013-09-06 | 2016-07-12 | President And Fellows Of Harvard College | Cas9-recombinase fusion proteins and uses thereof |
| US9526784B2 (en) | 2013-09-06 | 2016-12-27 | President And Fellows Of Harvard College | Delivery system for functional nucleases |
| US9340799B2 (en) | 2013-09-06 | 2016-05-17 | President And Fellows Of Harvard College | MRNA-sensing switchable gRNAs |
| WO2015066119A1 (en) | 2013-10-30 | 2015-05-07 | North Carolina State University | Compositions and methods related to a type-ii crispr-cas system in lactobacillus buchneri |
| AU2014346559B2 (en) | 2013-11-07 | 2020-07-09 | Editas Medicine,Inc. | CRISPR-related methods and compositions with governing gRNAs |
| US20150165054A1 (en) | 2013-12-12 | 2015-06-18 | President And Fellows Of Harvard College | Methods for correcting caspase-9 point mutations |
| US11439712B2 (en) | 2014-04-08 | 2022-09-13 | North Carolina State University | Methods and compositions for RNA-directed repression of transcription using CRISPR-associated genes |
| GB201407852D0 (en) | 2014-05-02 | 2014-06-18 | Iontas Ltd | Preparation of libraries od protein variants expressed in eukaryotic cells and use for selecting binding molecules |
| WO2016022363A2 (en) | 2014-07-30 | 2016-02-11 | President And Fellows Of Harvard College | Cas9 proteins including ligand-dependent inteins |
| EP3633032A3 (en) | 2014-08-28 | 2020-07-29 | North Carolina State University | Novel cas9 proteins and guiding features for dna targeting and genome editing |
| EP3204496A1 (en) | 2014-10-10 | 2017-08-16 | Editas Medicine, Inc. | Compositions and methods for promoting homology directed repair |
| CA2963820A1 (en) | 2014-11-07 | 2016-05-12 | Editas Medicine, Inc. | Methods for improving crispr/cas-mediated genome-editing |
| ES2926467T3 (es) | 2015-04-13 | 2022-10-26 | Maxcyte Inc | Métodos y composiciones para la modificación del ADN genómico |
| KR20220139447A (ko) | 2015-05-29 | 2022-10-14 | 노쓰 캐롤라이나 스테이트 유니버시티 | 크리스퍼 핵산을 사용하여 박테리아, 고세균, 조류 및 효모를 스크리닝하는 방법 |
| KR102468240B1 (ko) | 2015-06-15 | 2022-11-17 | 노쓰 캐롤라이나 스테이트 유니버시티 | 핵산 및 rna-기반 항미생물제를 효율적으로 전달하기 위한 방법 및 조성물 |
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| KR20190031306A (ko) * | 2016-07-21 | 2019-03-25 | 맥스시티 인코포레이티드 | 게놈 dna를 변경하기 위한 방법 및 조성물 |
| CN115252807A (zh) * | 2016-07-27 | 2022-11-01 | 利兰斯坦福初级大学董事会 | 用于核酸递送的解体型细胞穿透性复合物 |
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| US11542509B2 (en) | 2016-08-24 | 2023-01-03 | President And Fellows Of Harvard College | Incorporation of unnatural amino acids into proteins using base editing |
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| US20130122591A1 (en) * | 2011-10-27 | 2013-05-16 | The Regents Of The University Of California | Methods and compositions for modification of the hprt locus |
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2015
- 2015-04-13 EP EP15780038.4A patent/EP3132025B1/en active Active
- 2015-04-13 US US15/303,932 patent/US20170029805A1/en not_active Abandoned
- 2015-04-13 ES ES15780038T patent/ES2962509T3/es active Active
- 2015-04-13 WO PCT/US2015/025523 patent/WO2015160683A1/en not_active Ceased
- 2015-04-13 JP JP2016562503A patent/JP2017513477A/ja active Pending
- 2015-04-13 KR KR1020167031636A patent/KR102406585B1/ko active Active
- 2015-04-13 SG SG11201608503UA patent/SG11201608503UA/en unknown
- 2015-04-13 CN CN201580025136.0A patent/CN106687585B/zh active Active
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2020
- 2020-07-29 JP JP2020127917A patent/JP7209671B2/ja active Active
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Also Published As
| Publication number | Publication date |
|---|---|
| CN106687585B (zh) | 2021-11-02 |
| EP3132025B1 (en) | 2023-08-30 |
| WO2015160683A1 (en) | 2015-10-22 |
| JP2021010366A (ja) | 2021-02-04 |
| EP3132025A1 (en) | 2017-02-22 |
| CN106687585A (zh) | 2017-05-17 |
| ES2962509T3 (es) | 2024-03-19 |
| SG11201608503UA (en) | 2016-11-29 |
| KR20170026332A (ko) | 2017-03-08 |
| KR102406585B1 (ko) | 2022-06-07 |
| EP3132025A4 (en) | 2017-10-18 |
| US20170029805A1 (en) | 2017-02-02 |
| JP7209671B2 (ja) | 2023-01-20 |
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