KR102370675B1 - 표적 핵산의 변형을 위한 개선된 방법 - Google Patents

표적 핵산의 변형을 위한 개선된 방법 Download PDF

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KR102370675B1
KR102370675B1 KR1020187030717A KR20187030717A KR102370675B1 KR 102370675 B1 KR102370675 B1 KR 102370675B1 KR 1020187030717 A KR1020187030717 A KR 1020187030717A KR 20187030717 A KR20187030717 A KR 20187030717A KR 102370675 B1 KR102370675 B1 KR 102370675B1
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KR20190002470A (ko
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린더트 더블유 뉴트붐
존 에이 맥엘버
로사 데 핀호 바로코
크리스 데 빌데
막스 파비앙 펠레
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바스프 플랜트 사이언스 컴퍼니 게엠베하
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KR1020187030717A 2016-04-29 2017-04-20 표적 핵산의 변형을 위한 개선된 방법 Active KR102370675B1 (ko)

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EP16167774 2016-04-29
EP16167773 2016-04-29
EP16167773.7 2016-04-29
EP16167774.5 2016-04-29
EP17156018 2017-02-14
EP17156018.8 2017-02-14
PCT/EP2017/059331 WO2017186550A1 (en) 2016-04-29 2017-04-20 Improved methods for modification of target nucleic acids

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KR1020237007132A Ceased KR20230038804A (ko) 2016-04-29 2017-04-20 표적 핵산의 변형을 위한 개선된 방법
KR1020227006922A Active KR102506185B1 (ko) 2016-04-29 2017-04-20 표적 핵산의 변형을 위한 개선된 방법

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KR1020227006922A Active KR102506185B1 (ko) 2016-04-29 2017-04-20 표적 핵산의 변형을 위한 개선된 방법

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US (5) US11608499B2 (enExample)
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CN (1) CN109072207B (enExample)
AU (5) AU2017256517B2 (enExample)
CA (1) CA3022475A1 (enExample)
CL (1) CL2018003074A1 (enExample)
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IL (1) IL262409B2 (enExample)
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Publication number Priority date Publication date Assignee Title
WO2013066438A2 (en) 2011-07-22 2013-05-10 President And Fellows Of Harvard College Evaluation and improvement of nuclease cleavage specificity
US9163284B2 (en) 2013-08-09 2015-10-20 President And Fellows Of Harvard College Methods for identifying a target site of a Cas9 nuclease
US9359599B2 (en) 2013-08-22 2016-06-07 President And Fellows Of Harvard College Engineered transcription activator-like effector (TALE) domains and uses thereof
US9340799B2 (en) 2013-09-06 2016-05-17 President And Fellows Of Harvard College MRNA-sensing switchable gRNAs
US9526784B2 (en) 2013-09-06 2016-12-27 President And Fellows Of Harvard College Delivery system for functional nucleases
US9322037B2 (en) 2013-09-06 2016-04-26 President And Fellows Of Harvard College Cas9-FokI fusion proteins and uses thereof
US20150166984A1 (en) 2013-12-12 2015-06-18 President And Fellows Of Harvard College Methods for correcting alpha-antitrypsin point mutations
AU2015298571B2 (en) 2014-07-30 2020-09-03 President And Fellows Of Harvard College Cas9 proteins including ligand-dependent inteins
EP3204496A1 (en) 2014-10-10 2017-08-16 Editas Medicine, Inc. Compositions and methods for promoting homology directed repair
WO2016073990A2 (en) 2014-11-07 2016-05-12 Editas Medicine, Inc. Methods for improving crispr/cas-mediated genome-editing
CA2999500A1 (en) 2015-09-24 2017-03-30 Editas Medicine, Inc. Use of exonucleases to improve crispr/cas-mediated genome editing
WO2017070632A2 (en) 2015-10-23 2017-04-27 President And Fellows Of Harvard College Nucleobase editors and uses thereof
WO2017165826A1 (en) 2016-03-25 2017-09-28 Editas Medicine, Inc. Genome editing systems comprising repair-modulating enzyme molecules and methods of their use
US11236313B2 (en) 2016-04-13 2022-02-01 Editas Medicine, Inc. Cas9 fusion molecules, gene editing systems, and methods of use thereof
JP2019517261A (ja) * 2016-06-01 2019-06-24 カーヴェーエス ザート ソシエタス・ヨーロピアKws Saat Se ゲノム操作のためのハイブリッド核酸配列
WO2018027078A1 (en) 2016-08-03 2018-02-08 President And Fellows Of Harard College Adenosine nucleobase editors and uses thereof
AU2017308889B2 (en) 2016-08-09 2023-11-09 President And Fellows Of Harvard College Programmable Cas9-recombinase fusion proteins and uses thereof
WO2018039438A1 (en) 2016-08-24 2018-03-01 President And Fellows Of Harvard College Incorporation of unnatural amino acids into proteins using base editing
KR20240007715A (ko) 2016-10-14 2024-01-16 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 핵염기 에디터의 aav 전달
US20190330620A1 (en) * 2016-10-14 2019-10-31 Emendobio Inc. Rna compositions for genome editing
WO2018094356A2 (en) * 2016-11-18 2018-05-24 Genedit Inc. Compositions and methods for target nucleic acid modification
WO2018119359A1 (en) 2016-12-23 2018-06-28 President And Fellows Of Harvard College Editing of ccr5 receptor gene to protect against hiv infection
EA201991809A1 (ru) * 2017-01-30 2020-02-05 КВС ЗААТ СЕ & КО. КГаА Сцепление матрицы для репарации с эндонуклеазами для геномной инженерии
KR20190123328A (ko) 2017-03-09 2019-10-31 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 암 백신
WO2018165504A1 (en) 2017-03-09 2018-09-13 President And Fellows Of Harvard College Suppression of pain by gene editing
CN110914310A (zh) 2017-03-10 2020-03-24 哈佛大学的校长及成员们 胞嘧啶至鸟嘌呤碱基编辑器
IL306092A (en) 2017-03-23 2023-11-01 Harvard College Nucleic base editors that include nucleic acid programmable DNA binding proteins
US11560566B2 (en) 2017-05-12 2023-01-24 President And Fellows Of Harvard College Aptazyme-embedded guide RNAs for use with CRISPR-Cas9 in genome editing and transcriptional activation
MY200418A (en) 2017-06-15 2023-12-25 Univ California Targeted non-viral dna insertions
EP3652312A1 (en) 2017-07-14 2020-05-20 Editas Medicine, Inc. Systems and methods for targeted integration and genome editing and detection thereof using integrated priming sites
EP3658573A1 (en) 2017-07-28 2020-06-03 President and Fellows of Harvard College Methods and compositions for evolving base editors using phage-assisted continuous evolution (pace)
EP3676376B1 (en) 2017-08-30 2025-01-15 President and Fellows of Harvard College High efficiency base editors comprising gam
KR102907044B1 (ko) 2017-10-02 2026-01-02 진에딧 인코포레이티드 변형된 cpf1 가이드 rna
CA3082251A1 (en) 2017-10-16 2019-04-25 The Broad Institute, Inc. Uses of adenosine base editors
SG11202003798TA (en) 2017-10-27 2020-05-28 Univ California Targeted replacement of endogenous t cell receptors
US12406749B2 (en) 2017-12-15 2025-09-02 The Broad Institute, Inc. Systems and methods for predicting repair outcomes in genetic engineering
US11268092B2 (en) 2018-01-12 2022-03-08 GenEdit, Inc. Structure-engineered guide RNA
WO2019226953A1 (en) 2018-05-23 2019-11-28 The Broad Institute, Inc. Base editors and uses thereof
EP3820495A4 (en) 2018-07-09 2022-07-20 The Broad Institute Inc. RNA-PROGRAMMABLE EPIGENETIC RNA MODIFIERS AND THEIR USES
AU2019327449B2 (en) 2018-08-28 2026-04-09 Flagship Pioneering Innovations Vi, Llc Methods and compositions for modulating a genome
WO2020092453A1 (en) 2018-10-29 2020-05-07 The Broad Institute, Inc. Nucleobase editors comprising geocas9 and uses thereof
KR102298105B1 (ko) 2019-01-08 2021-09-03 주식회사 엘지에너지솔루션 스웰링 게이지를 구비한 배터리 모듈 및 이를 포함하는 배터리 팩
US12351837B2 (en) 2019-01-23 2025-07-08 The Broad Institute, Inc. Supernegatively charged proteins and uses thereof
WO2020191241A1 (en) 2019-03-19 2020-09-24 The Broad Institute, Inc. Methods and compositions for editing nucleotide sequences
WO2020214842A1 (en) 2019-04-17 2020-10-22 The Broad Institute, Inc. Adenine base editors with reduced off-target effects
US12435330B2 (en) 2019-10-10 2025-10-07 The Broad Institute, Inc. Methods and compositions for prime editing RNA
KR20220149567A (ko) 2020-03-04 2022-11-08 바스프 에스이 이. 콜라이 및 바실루스에서의 발현을 위한 셔틀 벡터
EP4114954A1 (en) 2020-03-04 2023-01-11 Basf Se Method for the production of constitutive bacterial promoters conferring low to medium expression
IL296095A (en) 2020-03-04 2022-11-01 Flagship Pioneering Innovations Vi Llc Improved methods and compositions for genome modulation
CA3177481A1 (en) 2020-05-08 2021-11-11 David R. Liu Methods and compositions for simultaneous editing of both strands of a target double-stranded nucleotide sequence
CN112111505B (zh) * 2020-09-17 2022-03-15 江南大学 一种氧化葡萄糖酸杆菌中基因敲除的方法
WO2022251179A1 (en) * 2021-05-26 2022-12-01 Inscripta, Inc. Crispr editing in diploid genomes
EP4384616A4 (en) * 2021-08-11 2025-08-27 Univ Leland Stanford Junior HIGH-THROUGHPUT PRECISION GENOME EDITING IN HUMAN CELLS
MX2024003887A (es) 2021-10-14 2024-07-09 Arsenal Biosciences Inc Células inmunitarias que tienen arnch coespresados y sistemas de compuerta lógica.
US20250304934A1 (en) * 2021-12-14 2025-10-02 The Broad Institute, Inc. Reprogrammable fanzor polynucleotides and uses thereof
EP4486879A1 (en) 2022-03-01 2025-01-08 Wageningen Universiteit Cas12a nickases
CN114774420B (zh) * 2022-04-27 2023-09-22 扬州大学 一种水稻增强子及基于CRISPR/Cas9技术评估水稻增强子活性的方法
CN116179576B (zh) * 2022-10-25 2026-05-15 中国科学院微生物研究所 一种非典型毕赤酵母基因编辑系统及其应用

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2014150624A1 (en) * 2013-03-14 2014-09-25 Caribou Biosciences, Inc. Compositions and methods of nucleic acid-targeting nucleic acids
WO2016065364A1 (en) * 2014-10-24 2016-04-28 Life Technologies Corporation Compositions and methods for enhancing homologous recombination

Family Cites Families (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK0733059T3 (da) 1993-12-09 2000-10-16 Univ Jefferson Forbindelser og fremgangsmåder til site-rettede mutationer i eukaryote celler
US6027945A (en) 1997-01-21 2000-02-22 Promega Corporation Methods of isolating biological target materials using silica magnetic particles
US6555732B1 (en) 1998-09-14 2003-04-29 Pioneer Hi-Bred International, Inc. Rac-like genes and methods of use
EP1152058A1 (en) * 2000-05-03 2001-11-07 Institut Curie Methods and compositions for effecting homologous recombination
EP2325332B1 (en) 2005-08-26 2012-10-31 DuPont Nutrition Biosciences ApS Use of CRISPR associated genes (CAS)
SG11201510327TA (en) 2013-06-17 2016-01-28 Broad Inst Inc Delivery, engineering and optimization of systems, methods and compositions for targeting and modeling diseases and disorders of post mitotic cells
US11306328B2 (en) * 2013-07-26 2022-04-19 President And Fellows Of Harvard College Genome engineering
BR112016002851B1 (pt) 2013-08-12 2022-02-22 BASF Agro B.V. Molécula de ácido nucleico, constructo de ácido nucleico, vetor, polipeptídeo ppo, método para controlar vegetação indesejada e uso do ácido nucleico
CA2933002C (en) 2013-12-18 2023-08-15 BASF Agro B.V. Plants having an increased tolerance to protoporphyrinogen oxidase (ppo) herbicides
CA3208184A1 (en) * 2014-03-14 2015-09-17 Cibus Us Llc Methods and compositions for increasing efficiency of targeted gene modification using oligonucleotide-mediated gene repair
WO2015191693A2 (en) * 2014-06-10 2015-12-17 Massachusetts Institute Of Technology Method for gene editing
CN107614680A (zh) * 2015-05-14 2018-01-19 南加利福尼亚大学 利用重组核酸内切酶系统的最佳化基因编辑
US9790490B2 (en) * 2015-06-18 2017-10-17 The Broad Institute Inc. CRISPR enzymes and systems
WO2017024047A1 (en) 2015-08-03 2017-02-09 Emendobio Inc. Compositions and methods for increasing nuclease induced recombination rate in cells
WO2017064546A1 (en) * 2015-09-24 2017-04-20 Crispr Therapeutics Ag Novel family of rna-programmable endonucleases and their uses in genome editing and other applications
WO2017059241A1 (en) * 2015-10-02 2017-04-06 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Lentiviral protein delivery system for rna-guided genome editing
CN106811479B (zh) * 2015-11-30 2019-10-25 中国农业科学院作物科学研究所 利用CRISPR/Cas9系统定点修饰ALS基因获得抗除草剂水稻的系统及其应用
WO2017180711A1 (en) * 2016-04-13 2017-10-19 Editas Medicine, Inc. Grna fusion molecules, gene editing systems, and methods of use thereof
WO2018094356A2 (en) * 2016-11-18 2018-05-24 Genedit Inc. Compositions and methods for target nucleic acid modification

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2014150624A1 (en) * 2013-03-14 2014-09-25 Caribou Biosciences, Inc. Compositions and methods of nucleic acid-targeting nucleic acids
WO2016065364A1 (en) * 2014-10-24 2016-04-28 Life Technologies Corporation Compositions and methods for enhancing homologous recombination

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
Gene Therapy, 2002, Vol.9, pp.703-707 1부.*

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