CN109072207B - 用于修饰靶核酸的改进方法 - Google Patents
用于修饰靶核酸的改进方法 Download PDFInfo
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- CN109072207B CN109072207B CN201780025990.6A CN201780025990A CN109072207B CN 109072207 B CN109072207 B CN 109072207B CN 201780025990 A CN201780025990 A CN 201780025990A CN 109072207 B CN109072207 B CN 109072207B
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- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
- C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
- C12N15/905—Stable introduction of foreign DNA into chromosome using homologous recombination in yeast
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
- C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
- C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
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- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
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Applications Claiming Priority (7)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP16167774.5 | 2016-04-29 | ||
| EP16167773.7 | 2016-04-29 | ||
| EP16167774 | 2016-04-29 | ||
| EP16167773 | 2016-04-29 | ||
| EP17156018 | 2017-02-14 | ||
| EP17156018.8 | 2017-02-14 | ||
| PCT/EP2017/059331 WO2017186550A1 (en) | 2016-04-29 | 2017-04-20 | Improved methods for modification of target nucleic acids |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN109072207A CN109072207A (zh) | 2018-12-21 |
| CN109072207B true CN109072207B (zh) | 2024-05-07 |
Family
ID=58640844
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201780025990.6A Active CN109072207B (zh) | 2016-04-29 | 2017-04-20 | 用于修饰靶核酸的改进方法 |
Country Status (12)
| Country | Link |
|---|---|
| US (5) | US11608499B2 (enExample) |
| EP (5) | EP4166662A1 (enExample) |
| JP (2) | JP7184648B2 (enExample) |
| KR (3) | KR102506185B1 (enExample) |
| CN (1) | CN109072207B (enExample) |
| AU (5) | AU2017256517B2 (enExample) |
| CA (1) | CA3022475A1 (enExample) |
| CL (1) | CL2018003074A1 (enExample) |
| DK (1) | DK3448990T5 (enExample) |
| IL (1) | IL262409B2 (enExample) |
| MX (2) | MX388092B (enExample) |
| WO (1) | WO2017186550A1 (enExample) |
Families Citing this family (57)
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| CA2853829C (en) | 2011-07-22 | 2023-09-26 | President And Fellows Of Harvard College | Evaluation and improvement of nuclease cleavage specificity |
| US9163284B2 (en) | 2013-08-09 | 2015-10-20 | President And Fellows Of Harvard College | Methods for identifying a target site of a Cas9 nuclease |
| US9359599B2 (en) | 2013-08-22 | 2016-06-07 | President And Fellows Of Harvard College | Engineered transcription activator-like effector (TALE) domains and uses thereof |
| US9340800B2 (en) | 2013-09-06 | 2016-05-17 | President And Fellows Of Harvard College | Extended DNA-sensing GRNAS |
| US9322037B2 (en) | 2013-09-06 | 2016-04-26 | President And Fellows Of Harvard College | Cas9-FokI fusion proteins and uses thereof |
| US9526784B2 (en) | 2013-09-06 | 2016-12-27 | President And Fellows Of Harvard College | Delivery system for functional nucleases |
| US9840699B2 (en) | 2013-12-12 | 2017-12-12 | President And Fellows Of Harvard College | Methods for nucleic acid editing |
| WO2016022363A2 (en) | 2014-07-30 | 2016-02-11 | President And Fellows Of Harvard College | Cas9 proteins including ligand-dependent inteins |
| CA2963693A1 (en) | 2014-10-10 | 2016-04-14 | Editas Medicine, Inc. | Compositions and methods for promoting homology directed repair |
| AU2015342749B2 (en) | 2014-11-07 | 2022-01-27 | Editas Medicine, Inc. | Methods for improving CRISPR/Cas-mediated genome-editing |
| CA2999500A1 (en) | 2015-09-24 | 2017-03-30 | Editas Medicine, Inc. | Use of exonucleases to improve crispr/cas-mediated genome editing |
| IL294014B2 (en) | 2015-10-23 | 2024-07-01 | Harvard College | Nucleobase editors and uses thereof |
| US11597924B2 (en) | 2016-03-25 | 2023-03-07 | Editas Medicine, Inc. | Genome editing systems comprising repair-modulating enzyme molecules and methods of their use |
| EP3443086B1 (en) | 2016-04-13 | 2021-11-24 | Editas Medicine, Inc. | Cas9 fusion molecules, gene editing systems, and methods of use thereof |
| CN109642232A (zh) * | 2016-06-01 | 2019-04-16 | Kws种子欧洲股份公司 | 用于基因组改造的杂合核酸序列 |
| AU2017306676B2 (en) | 2016-08-03 | 2024-02-22 | President And Fellows Of Harvard College | Adenosine nucleobase editors and uses thereof |
| EP3497214B1 (en) | 2016-08-09 | 2023-06-28 | President and Fellows of Harvard College | Programmable cas9-recombinase fusion proteins and uses thereof |
| US11542509B2 (en) | 2016-08-24 | 2023-01-03 | President And Fellows Of Harvard College | Incorporation of unnatural amino acids into proteins using base editing |
| WO2018071868A1 (en) | 2016-10-14 | 2018-04-19 | President And Fellows Of Harvard College | Aav delivery of nucleobase editors |
| US20190330620A1 (en) * | 2016-10-14 | 2019-10-31 | Emendobio Inc. | Rna compositions for genome editing |
| EP3541945A4 (en) * | 2016-11-18 | 2020-12-09 | Genedit Inc. | COMPOSITIONS AND METHODS OF MODIFICATION OF TARGET NUCLEIC ACIDS |
| WO2018119359A1 (en) | 2016-12-23 | 2018-06-28 | President And Fellows Of Harvard College | Editing of ccr5 receptor gene to protect against hiv infection |
| WO2018138385A1 (en) * | 2017-01-30 | 2018-08-02 | Kws Saat Se | Repair template linkage to endonucleases for genome engineering |
| EP3592853A1 (en) | 2017-03-09 | 2020-01-15 | President and Fellows of Harvard College | Suppression of pain by gene editing |
| WO2018165631A1 (en) | 2017-03-09 | 2018-09-13 | President And Fellows Of Harvard College | Cancer vaccine |
| WO2018165629A1 (en) | 2017-03-10 | 2018-09-13 | President And Fellows Of Harvard College | Cytosine to guanine base editor |
| KR20240116572A (ko) | 2017-03-23 | 2024-07-29 | 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 | 핵산 프로그램가능한 dna 결합 단백질을 포함하는 핵염기 편집제 |
| US11560566B2 (en) | 2017-05-12 | 2023-01-24 | President And Fellows Of Harvard College | Aptazyme-embedded guide RNAs for use with CRISPR-Cas9 in genome editing and transcriptional activation |
| CA3067382A1 (en) | 2017-06-15 | 2018-12-20 | The Regents Of The University Of California | Targeted non-viral dna insertions |
| EP3652312A1 (en) | 2017-07-14 | 2020-05-20 | Editas Medicine, Inc. | Systems and methods for targeted integration and genome editing and detection thereof using integrated priming sites |
| CN111801345A (zh) | 2017-07-28 | 2020-10-20 | 哈佛大学的校长及成员们 | 使用噬菌体辅助连续进化(pace)的进化碱基编辑器的方法和组合物 |
| US11319532B2 (en) | 2017-08-30 | 2022-05-03 | President And Fellows Of Harvard College | High efficiency base editors comprising Gam |
| WO2019079347A1 (en) | 2017-10-16 | 2019-04-25 | The Broad Institute, Inc. | USES OF BASIC EDITORS ADENOSINE |
| IL305485B2 (en) | 2017-10-27 | 2025-10-01 | Univ California | Targeted replacement of endogenous T cell receptors |
| US12406749B2 (en) | 2017-12-15 | 2025-09-02 | The Broad Institute, Inc. | Systems and methods for predicting repair outcomes in genetic engineering |
| US11268092B2 (en) | 2018-01-12 | 2022-03-08 | GenEdit, Inc. | Structure-engineered guide RNA |
| EP3797160A1 (en) | 2018-05-23 | 2021-03-31 | The Broad Institute Inc. | Base editors and uses thereof |
| WO2020014261A1 (en) | 2018-07-09 | 2020-01-16 | The Broad Institute, Inc. | Rna programmable epigenetic rna modifiers and uses thereof |
| WO2020047124A1 (en) | 2018-08-28 | 2020-03-05 | Flagship Pioneering, Inc. | Methods and compositions for modulating a genome |
| WO2020092453A1 (en) | 2018-10-29 | 2020-05-07 | The Broad Institute, Inc. | Nucleobase editors comprising geocas9 and uses thereof |
| KR102298105B1 (ko) | 2019-01-08 | 2021-09-03 | 주식회사 엘지에너지솔루션 | 스웰링 게이지를 구비한 배터리 모듈 및 이를 포함하는 배터리 팩 |
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| EP4114956A1 (en) | 2020-03-04 | 2023-01-11 | Basf Se | Shuttle vector for expression in e. coli and bacilli |
| CN115605597A (zh) | 2020-03-04 | 2023-01-13 | 巴斯夫欧洲公司(De) | 用于产生赋予低至中等表达的组成型细菌启动子的方法 |
| AU2021267940A1 (en) | 2020-05-08 | 2022-12-08 | President And Fellows Of Harvard College | Methods and compositions for simultaneous editing of both strands of a target double-stranded nucleotide sequence |
| CN112111505B (zh) * | 2020-09-17 | 2022-03-15 | 江南大学 | 一种氧化葡萄糖酸杆菌中基因敲除的方法 |
| WO2022251179A1 (en) * | 2021-05-26 | 2022-12-01 | Inscripta, Inc. | Crispr editing in diploid genomes |
| US20240263173A1 (en) * | 2021-08-11 | 2024-08-08 | The Board Of Trustees Of The Leland Stanford Junior University | High-throughput precision genome editing in human cells |
| AU2022366987A1 (en) | 2021-10-14 | 2024-05-16 | Arsenal Biosciences, Inc. | Immune cells having co-expressed shrnas and logic gate systems |
| US20250304934A1 (en) * | 2021-12-14 | 2025-10-02 | The Broad Institute, Inc. | Reprogrammable fanzor polynucleotides and uses thereof |
| AU2023228036A1 (en) | 2022-03-01 | 2024-09-12 | BASF Agricultural Solutions Seed US LLC | Cas12a nickases |
| CN114774420B (zh) * | 2022-04-27 | 2023-09-22 | 扬州大学 | 一种水稻增强子及基于CRISPR/Cas9技术评估水稻增强子活性的方法 |
| CN116179576A (zh) * | 2022-10-25 | 2023-05-30 | 中国科学院微生物研究所 | 一种非典型毕赤酵母基因编辑系统及其应用 |
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| AU2014281030B2 (en) | 2013-06-17 | 2020-07-09 | Massachusetts Institute Of Technology | Delivery, engineering and optimization of systems, methods and compositions for targeting and modeling diseases and disorders of post mitotic cells |
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| WO2017059241A1 (en) * | 2015-10-02 | 2017-04-06 | The United States Of America, As Represented By The Secretary, Department Of Health And Human Services | Lentiviral protein delivery system for rna-guided genome editing |
| CN106811479B (zh) | 2015-11-30 | 2019-10-25 | 中国农业科学院作物科学研究所 | 利用CRISPR/Cas9系统定点修饰ALS基因获得抗除草剂水稻的系统及其应用 |
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