KR102358807B1 - 대사적으로 최적화된 세포 배양 - Google Patents

대사적으로 최적화된 세포 배양 Download PDF

Info

Publication number
KR102358807B1
KR102358807B1 KR1020217011547A KR20217011547A KR102358807B1 KR 102358807 B1 KR102358807 B1 KR 102358807B1 KR 1020217011547 A KR1020217011547 A KR 1020217011547A KR 20217011547 A KR20217011547 A KR 20217011547A KR 102358807 B1 KR102358807 B1 KR 102358807B1
Authority
KR
South Korea
Prior art keywords
cell culture
cell
cells
culture
lactate
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
KR1020217011547A
Other languages
English (en)
Korean (ko)
Other versions
KR20210046835A (ko
Inventor
숀 로렌스
앤 킴
에이미 죤슨
Original Assignee
리제너론 파마슈티칼스 인코포레이티드
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=52813657&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=KR102358807(B1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by 리제너론 파마슈티칼스 인코포레이티드 filed Critical 리제너론 파마슈티칼스 인코포레이티드
Priority to KR1020227003381A priority Critical patent/KR102510238B1/ko
Publication of KR20210046835A publication Critical patent/KR20210046835A/ko
Application granted granted Critical
Publication of KR102358807B1 publication Critical patent/KR102358807B1/ko
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10Cells modified by introduction of foreign genetic material
    • C12N5/12Fused cells, e.g. hybridomas
    • C12N5/16Animal cells
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K16/00Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • C12N5/0681Cells of the genital tract; Non-germinal cells from gonads
    • C12N5/0682Cells of the female genital tract, e.g. endometrium; Non-germinal cells from ovaries, e.g. ovarian follicle cells
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00Preparation of peptides or proteins
    • C12P21/02Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2317/00Immunoglobulins specific features
    • C07K2317/10Immunoglobulins specific features characterized by their source of isolation or production
    • C07K2317/14Specific host cells or culture conditions, e.g. components, pH or temperature
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00Specific components of cell culture medium
    • C12N2500/60Buffer, e.g. pH regulation, osmotic pressure
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2511/00Cells for large scale production

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Cell Biology (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Reproductive Health (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Peptides Or Proteins (AREA)
KR1020217011547A 2013-10-11 2014-10-10 대사적으로 최적화된 세포 배양 Active KR102358807B1 (ko)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR1020227003381A KR102510238B1 (ko) 2013-10-11 2014-10-10 대사적으로 최적화된 세포 배양

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US201361889815P 2013-10-11 2013-10-11
US61/889,815 2013-10-11
KR1020167009163A KR102243980B1 (ko) 2013-10-11 2014-10-10 대사적으로 최적화된 세포 배양
PCT/US2014/059993 WO2015054554A1 (en) 2013-10-11 2014-10-10 Metabolically optimized cell culture

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
KR1020167009163A Division KR102243980B1 (ko) 2013-10-11 2014-10-10 대사적으로 최적화된 세포 배양

Related Child Applications (1)

Application Number Title Priority Date Filing Date
KR1020227003381A Division KR102510238B1 (ko) 2013-10-11 2014-10-10 대사적으로 최적화된 세포 배양

Publications (2)

Publication Number Publication Date
KR20210046835A KR20210046835A (ko) 2021-04-28
KR102358807B1 true KR102358807B1 (ko) 2022-02-08

Family

ID=52813657

Family Applications (4)

Application Number Title Priority Date Filing Date
KR1020227003381A Active KR102510238B1 (ko) 2013-10-11 2014-10-10 대사적으로 최적화된 세포 배양
KR1020217011547A Active KR102358807B1 (ko) 2013-10-11 2014-10-10 대사적으로 최적화된 세포 배양
KR1020237008467A Active KR102610425B1 (ko) 2013-10-11 2014-10-10 대사적으로 최적화된 세포 배양
KR1020167009163A Active KR102243980B1 (ko) 2013-10-11 2014-10-10 대사적으로 최적화된 세포 배양

Family Applications Before (1)

Application Number Title Priority Date Filing Date
KR1020227003381A Active KR102510238B1 (ko) 2013-10-11 2014-10-10 대사적으로 최적화된 세포 배양

Family Applications After (2)

Application Number Title Priority Date Filing Date
KR1020237008467A Active KR102610425B1 (ko) 2013-10-11 2014-10-10 대사적으로 최적화된 세포 배양
KR1020167009163A Active KR102243980B1 (ko) 2013-10-11 2014-10-10 대사적으로 최적화된 세포 배양

Country Status (17)

Country Link
US (2) US20160244725A1 (enExample)
EP (3) EP3812453A1 (enExample)
JP (3) JP6663854B2 (enExample)
KR (4) KR102510238B1 (enExample)
CN (2) CN105637085A (enExample)
AU (2) AU2014331776B2 (enExample)
CA (1) CA2926049C (enExample)
DK (2) DK3055409T3 (enExample)
EA (2) EA201991976A1 (enExample)
ES (2) ES2678945T3 (enExample)
HU (2) HUE051049T2 (enExample)
IL (2) IL244511A0 (enExample)
MX (2) MX384960B (enExample)
PL (2) PL3351620T3 (enExample)
SG (2) SG10201800772TA (enExample)
WO (1) WO2015054554A1 (enExample)
ZA (1) ZA201601550B (enExample)

Families Citing this family (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11390663B2 (en) 2013-10-11 2022-07-19 Regeneron Pharmaceuticals, Inc. Metabolically optimized cell culture
EA201991976A1 (ru) 2013-10-11 2020-04-02 Регенерон Фармасьютикалз, Инк. Метаболически оптимизированная клеточная культура
US20160244726A1 (en) * 2013-10-11 2016-08-25 Regeneron Pharmaceuticals, Inc. Metabolically optimized cell culture
JP7260020B2 (ja) * 2017-03-31 2023-04-18 株式会社三洋物産 遊技機
JP7260011B2 (ja) * 2017-03-31 2023-04-18 株式会社三洋物産 遊技機
JP7260018B2 (ja) * 2017-03-31 2023-04-18 株式会社三洋物産 遊技機
JP7260021B2 (ja) * 2017-04-27 2023-04-18 株式会社三洋物産 遊技機
JP7310947B2 (ja) * 2017-04-27 2023-07-19 株式会社三洋物産 遊技機
JP7310945B2 (ja) * 2017-04-27 2023-07-19 株式会社三洋物産 遊技機
JP7310952B2 (ja) * 2017-04-27 2023-07-19 株式会社三洋物産 遊技機
JP7260012B2 (ja) * 2017-04-27 2023-04-18 株式会社三洋物産 遊技機
JP7260013B2 (ja) * 2017-04-27 2023-04-18 株式会社三洋物産 遊技機
JP7272480B2 (ja) * 2017-04-27 2023-05-12 株式会社三洋物産 遊技機
JP7260009B2 (ja) * 2017-04-27 2023-04-18 株式会社三洋物産 遊技機
IL273549B2 (en) 2017-10-06 2023-10-01 Lonza Ag Automated control of cell culture using Raman spectroscopy
CN118580927A (zh) 2017-10-16 2024-09-03 里珍纳龙药品有限公司 灌注生物反应器及相关使用方法
JP7471220B2 (ja) * 2017-11-30 2024-04-19 エフ・ホフマン-ラ・ロシュ・アクチェンゲゼルシャフト 哺乳動物細胞の培養方法
JP2021519073A (ja) * 2018-03-29 2021-08-10 ジェネンテック, インコーポレイテッド 哺乳動物細胞におけるラクトジェニック活性の制御
GB201810772D0 (en) 2018-06-29 2018-08-15 Ge Healthcare Bio Sciences Ab Method in bioprocess purification system
JP7231355B2 (ja) * 2018-08-22 2023-03-01 日機装株式会社 細胞培養方法および細胞培養装置
EA202092684A1 (ru) 2018-08-27 2021-03-11 Ридженерон Фармасьютикалз, Инк. Применение рамановской спектроскопии для последующей очистки
JP2020151560A (ja) * 2020-06-24 2020-09-24 株式会社三洋物産 遊技機
JP2020151559A (ja) * 2020-06-24 2020-09-24 株式会社三洋物産 遊技機
EP3985097A1 (en) * 2020-10-14 2022-04-20 Sartorius Stedim Biotech GmbH A method and system for configuring and/or setup of a downstream process for processing a biomass

Family Cites Families (26)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5703055A (en) 1989-03-21 1997-12-30 Wisconsin Alumni Research Foundation Generation of antibodies through lipid mediated DNA delivery
KR970029803A (ko) 1995-11-03 1997-06-26 김광호 반도체 메모리장치의 프리차지 회로
US6165741A (en) 1997-05-30 2000-12-26 The Trustees Of The University Of Pennsylvania Method for rapid detection of bacterial growth in cultures
US5972650A (en) 1997-06-26 1999-10-26 Brigham And Women's Hospital Tetracycline repressor regulated mammalian cell transcription and viral replication switch
WO2002057423A2 (en) 2001-01-16 2002-07-25 Regeneron Pharmaceuticals, Inc. Isolating cells expressing secreted proteins
US6953692B2 (en) * 2001-12-18 2005-10-11 Bayer Pharmaceuticals Corporation Device and method for seed-train expansion of mammalian cells
US7039770B1 (en) 2002-03-05 2006-05-02 Juniper Networks, Inc. Low latency request dispatcher
CA2417689C (en) 2002-03-05 2006-05-09 F. Hoffmann-La Roche Ag Improved methods for growing mammalian cells in vitro
US8673589B2 (en) 2002-05-29 2014-03-18 Regeneron Pharmaceuticals, Inc. Inducible eukaryotic expression system
EP1623019B2 (en) 2003-05-15 2017-01-25 Wyeth LLC Restricted glucose feed for animal cell culture
US7531327B2 (en) * 2004-07-23 2009-05-12 Immunomedics, Inc. Methods and compositions for increasing longevity and protein yield from a cell culture
US7335491B2 (en) 2004-08-27 2008-02-26 Wyeth Research Ireland Limited Production of anti-abeta
US7300773B2 (en) * 2004-08-27 2007-11-27 Wyeth Research Ireland Limited Production of TNFR-Ig
JP2009514532A (ja) * 2005-11-02 2009-04-09 ワイス 哺乳動物細胞を適応させるための方法
US10155816B2 (en) 2005-11-28 2018-12-18 Genmab A/S Recombinant monovalent antibodies and methods for production thereof
US7575890B2 (en) 2006-01-18 2009-08-18 Oxygen Enterprises, Ltd. Method for rapid detection and evaluation of cultured cell growth
ES2541546T3 (es) 2006-11-03 2015-07-21 Wyeth Llc Sustancias que inhiben la glucólisis en cultivo celular
EP2150617B1 (en) 2007-06-04 2014-10-22 Regeneron Pharmaceuticals, Inc. Enhanced expression and stability regions
US20090042253A1 (en) 2007-08-09 2009-02-12 Wyeth Use of perfusion to enhance production of fed-batch cell culture in bioreactors
ES2557903T3 (es) * 2008-06-13 2016-01-29 Janssen Biotech, Inc. Métodos para obtener una alta densidad celular viable en cultivos celulares de mamíferos
US8470552B2 (en) * 2009-10-12 2013-06-25 Keck Graduate Institute Strategy to reduce lactic acid production and control PH in animal cell culture
RU2644651C2 (ru) * 2010-04-26 2018-02-13 Новартис Аг Среда для культивирования клеток
KR20190067277A (ko) * 2010-05-28 2019-06-14 제넨테크, 인크. 락테이트 데히드로게나제 및 피루베이트 데히드로게나제 키나제의 발현의 하향조절에 의한 락테이트 수준의 감소 및 폴리펩티드 생산의 증가
TWI637057B (zh) 2012-11-09 2018-10-01 拜爾沙納有限公司 具交替生物反應器用途之不連續進料批次製程
US20150353542A1 (en) * 2013-01-14 2015-12-10 Amgen Inc. Methods of using cell-cycle inhibitors to modulate one or more properties of a cell culture
EA201991976A1 (ru) 2013-10-11 2020-04-02 Регенерон Фармасьютикалз, Инк. Метаболически оптимизированная клеточная культура

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
BHANU CHANDRA MULUKUTLA et al., Metabolic Engineering (2011) 14(2):138-149
FRANCESCA ZAGARI et al., New Biotechnology (2013) 30(2):238-245

Also Published As

Publication number Publication date
IL262833A (en) 2018-12-31
EA201991976A1 (ru) 2020-04-02
MX2016004564A (es) 2016-07-12
KR102610425B1 (ko) 2023-12-06
AU2014331776A1 (en) 2016-03-24
WO2015054554A1 (en) 2015-04-16
KR20160062032A (ko) 2016-06-01
KR102510238B1 (ko) 2023-03-16
EP3055409A4 (en) 2016-09-28
EP3055409B1 (en) 2018-04-25
PL3351620T3 (pl) 2021-03-08
HK1253275A1 (en) 2019-06-14
ES2833471T3 (es) 2021-06-15
HUE051049T2 (hu) 2021-01-28
KR20210046835A (ko) 2021-04-28
EA033783B1 (ru) 2019-11-25
JP7377243B2 (ja) 2023-11-09
JP2022009453A (ja) 2022-01-14
KR20220020415A (ko) 2022-02-18
AU2021200193A1 (en) 2021-03-18
JP6967620B2 (ja) 2021-11-17
EP3351620B1 (en) 2020-09-02
HUE039551T2 (hu) 2019-01-28
US20210087535A1 (en) 2021-03-25
HK1223122A1 (en) 2017-07-21
KR20230038324A (ko) 2023-03-17
US20160244725A1 (en) 2016-08-25
MX384960B (es) 2025-03-14
AU2014331776B2 (en) 2020-10-22
JP2020072760A (ja) 2020-05-14
CA2926049A1 (en) 2015-04-16
ZA201601550B (en) 2023-05-31
SG10201800772TA (en) 2018-03-28
CA2926049C (en) 2023-09-05
KR102243980B1 (ko) 2021-04-26
DK3351620T3 (da) 2020-10-26
EP3812453A1 (en) 2021-04-28
SG11201601681QA (en) 2016-04-28
EP3351620A1 (en) 2018-07-25
MX2020011334A (es) 2021-07-30
DK3055409T3 (en) 2018-07-30
CN105637085A (zh) 2016-06-01
PL3055409T3 (pl) 2018-10-31
JP6663854B2 (ja) 2020-03-13
JP2016532457A (ja) 2016-10-20
CN114717196A (zh) 2022-07-08
MX376623B (es) 2025-03-07
EA201690600A1 (ru) 2016-09-30
AU2021200193B2 (en) 2023-08-24
EP3055409A1 (en) 2016-08-17
ES2678945T3 (es) 2018-08-21
IL244511A0 (en) 2016-04-21

Similar Documents

Publication Publication Date Title
JP7377243B2 (ja) 代謝が最適化された細胞培養
US20250326820A1 (en) Metabolically optimized cell culture
US20160244726A1 (en) Metabolically optimized cell culture
HK40042432A (en) Metabolically optimized cell culture
HK1253275B (en) Metabolically optimized cell culture
HK1223122B (en) Metabolically optimized cell culture
EA040764B1 (ru) Метаболически оптимизированная клеточная культура
EA045030B1 (ru) Метаболически оптимизированная клеточная культура
BR122024020665A2 (pt) Método para cultivar células, e, célula hospedeira metabolicamente alterada
BR122024020669A2 (pt) Método para cultivar células, e, célula hospedeira metabolicamente alterada
BR112016007739B1 (pt) Método para cultivar células

Legal Events

Date Code Title Description
A107 Divisional application of patent
A201 Request for examination
PA0104 Divisional application for international application

Comment text: Divisional Application for International Patent

Patent event code: PA01041R01D

Patent event date: 20210419

Application number text: 1020167009163

Filing date: 20160406

PA0201 Request for examination

Patent event code: PA02012R01D

Patent event date: 20210419

Comment text: Request for Examination of Application

PG1501 Laying open of application
E902 Notification of reason for refusal
PE0902 Notice of grounds for rejection

Comment text: Notification of reason for refusal

Patent event date: 20210615

Patent event code: PE09021S01D

E701 Decision to grant or registration of patent right
PE0701 Decision of registration

Patent event code: PE07011S01D

Comment text: Decision to Grant Registration

Patent event date: 20211028

GRNT Written decision to grant
PR0701 Registration of establishment

Comment text: Registration of Establishment

Patent event date: 20220128

Patent event code: PR07011E01D

PR1002 Payment of registration fee

Payment date: 20220203

End annual number: 3

Start annual number: 1

PG1601 Publication of registration
PR1001 Payment of annual fee

Payment date: 20241224

Start annual number: 4

End annual number: 4