KR102347109B1 - Cosmetic composition for improvement of moisturizing with hydrolyzed plant extract - Google Patents

Abstract

The present invention relates to a moisturizing cosmetic composition containing hydrolysate extracts of Hordeum vulgare, Gardenia jasminoides, and Viola tricolor. The cosmetic composition of the present invention exhibits excellent cellular moisturizing and intercellular moisturizing effects. Also, the cosmetic composition of the present invention has excellent stability and is harmless to human body by using natural materials.

Description

Moisturizing cosmetic composition containing hydrolyzed plant extract {Cosmetic composition for improvement of moisturizing with hydrolyzed plant extract}

The present invention relates to a moisturizing cosmetic composition containing a hydrolyzed plant extract, and more particularly, to a moisturizing cosmetic composition containing a malt, gardenia Florida , and Viola Tricolor hydrolyzate extract. will be.

The skin not only has the largest surface area among human body organs, but is also the outermost organ. It prevents the loss of biological components such as water and electrolytes in the human body, and at the same time prevents the intrusion of harmful substances from the outside. . The structure of the skin is divided into three layers: the epidermis, the dermis, and the subcutaneous tissue in order from the outside.

The moisture content of the skin is about 70% in the dermis layer, but decreases toward the epidermis layer and becomes about 10% to 30% in the epidermis layer. When the moisture content of the epidermis layer is less than 10%, the skin becomes rough and the protective function of the body is lost, and aging occurs. The epidermis is divided into four layers from the outside: the stratum corneum, granular layer, stratum corneum, and basal layer. It has barrier function. The stratum corneum is filled with flat keratinocytes and intercellular lipids between them, and forms a lamellar shape. It is well known that the moisture content of the stratum corneum of the skin is an important factor determining the flexibility and luster of the skin.

In addition, a high concentration of Natural Moisturizing Factor (NMF) exists in the keratinocytes of a healthy person, which helps the skin to contain moisture. However, despite these functions of the skin, extrinsic aging caused by external environments such as indoor heating, polluted air and ultraviolet rays, intrinsic aging, and loss of skin components due to physical and chemical stimuli such as friction, shaving and face washing An imbalance can lead to dry skin. To prevent this, the need for a skin moisturizer is important, and various studies are being conducted to confirm the skin moisturizing effect at the cellular level.

Korean Patent No. 10-1987167 (June 3, 2019) describes a cosmetic composition for skin moisturizing and skin barrier strengthening, comprising a hydrolase-treated corncob, malt and flaxseed complex extract. Korean Patent No. 10-1819060 (2018.01.10.) describes a cosmetic composition for antioxidant, wrinkle improvement and whitening containing fermented Hwangryeonhaedoktang (hwangryeon, hwangbaek, golden, gardenia) as an active ingredient. . Korean Patent No. 10-0858449 (2008.09.08.) describes a skin moisturizing and anti-wrinkle cosmetic composition containing a tricolor violet extract stabilized with nanoliposomes.

The present invention aims to develop and provide a cosmetic composition made of natural material that is harmless to the human body, has excellent stability, and is effective for moisturizing, for the purpose of solving the problems of chemical substances used in existing moisturizing cosmetic compositions.

The present invention provides a cosmetic composition for moisturizing the skin comprising a hydrolyzed malt extract, a hydrolyzed Gardenia Florida extract, and a hydrolyzed Viola Tricolor extract.

In the cosmetic composition for moisturizing the skin of the present invention, the moisturizing agent preferably includes at least one selected from cellular moisturizing and intercellular moisturizing.

In the cosmetic composition for moisturizing the skin of the present invention, the extraction may be, for example, extraction through any one extraction method selected from a solvent extraction method, a supercritical extraction method, and an ultrasonic extraction method.

In this case, the solvent extraction method is, for example, water, anhydrous or hydrous lower alcohol having 1-4 carbon atoms, propylene glycol, butylene glycol, glycerin, acetone, ethyl acetate, chloroform, butyl acetate, diethyl ether, dichloromethane, hexane or Any one extraction solvent selected from the group consisting of mixtures thereof may be used.

The cosmetic composition of the present invention exhibits an excellent effect in moisturizing cells and intercellular moisturizing. In addition, the cosmetic composition of the present invention is harmless to the human body by using a natural material, and has excellent stability.

1 is a result of measuring the toxicity of the extracts of Comparative Examples 1 to 3 or a mixture of Comparative Examples 4 to 6 and Example 1 on cells.
2 is a result of confirming the cell moisturizing effect through the control of Aquaporin-3 expression of the extracts of Comparative Examples 1 to 3 or the mixture of Comparative Examples 4 to 6 and Example 1.
3 is a result confirming the skin barrier moisturizing effect through control of Claudin-1 expression of the extracts of Comparative Examples 1 to 3 or the mixture of Comparative Examples 4 to 6 and Example 1.
4 is a result showing the moisture reduction rate of the extracts of Comparative Examples 1 to 3 or the mixture of Comparative Examples 4 to 6 and Example 1.
5 is a result of measuring the skin moisture content when applying Formulation Example 1 and Comparative Formulation Example 1.
6 is a result of measuring the amount of transdermal water loss when applying Formulation Example 1 and Comparative Formulation Example 1.

It is very important to properly maintain the moisture content of the stratum corneum in the skin. For this purpose, a component similar to sebum, an NMF component, or a moisturizing agent such as polyol is added to cosmetics. For example, as water-soluble polyols, glycerin and solitol having three or more hydroxyl groups (OH groups) show excellent moisturizing power, but are sticky and cause discomfort when used, and propylene glycol with two hydroxyl groups, 1,3-butylene Glycol, etc., may cause side effects on the skin. In addition, other natural moisturizing factors, such as sodium pyrrolidone carboxylate (PCA-Na), sodium lactate, and urea, have strong electrolytic properties, which impair the emulsion stability of cosmetics. Although it has the ability, there is a limit to its moisturizing ability, so it is necessary to develop a natural cosmetic composition that is safe and enhances skin moisturizing power.

Accordingly, the present invention provides a cosmetic composition for moisturizing the skin comprising a hydrolyzed malt extract, a hydrolyzed Gardenia Florida extract, and a hydrolyzed Viola Tricolor extract.

Malt is produced by germinating Hordeum vulgare Linn (大麥) of the Grape family to produce amylase, a malt enzyme. Malt is effective for digestive disorders caused by weak stomach, especially for indigestion of wheat flour. In addition, it is effective in the lack of milk secretion. There is a young shoot at one end and a young root at the other end, the outer surface is pale yellow, and the endosperm is milky white. The vagina is hard and brittle. Synonyms for malt include malt malt, aorta wall, barley bran, gwangmaekul, and grain malt.

Gardenia ( Gardenia Florida ) refers to the fruits of the gardenia tree belonging to the genus Apiaceae, which is an evergreen shrub, or dried ones. Gardenia grows wild in the deep mountains of hot regions such as central and southern Korea, China, Taiwan, and Japan. The fruit is about 3.5cm long and ripens to yellowish red in September, and is used not only medicinally but also as a dye. Pharmacologically, it is known to be effective for diabetes, hypertension, insomnia, jaundice, urinary disorders, eye congestion, conjunctivitis, hematemesis, uterine bleeding, hematuria, and bruises. It also treats joint bruises and has a detoxifying effect. Gardenia can be consumed alone or in combination with other medicinal substances in water, or powdered gardenia and mixed with water and attached to the affected area.

The tricolor violet ( Viola Tricolor ) is also known as pansy, as it is an annual herb of the order Viola Tricolor. The height is 10~15cm, and the stem grows straight or stretches sideways, and the branches are divided a lot. The lower leaves are round egg-shaped, and the upper leaves are slightly thin and long spatula-shaped. The petiole has long and large stipules. In March~June, a long peduncle comes out from the leaf axil and hangs one by one at the end. There are 5 petals, and the diameter of the flower is 3~12cm, and there are small, middle, and large species. There are three colors of white, yellow, and purple, and the horticultural varieties of Pina have a single color, but there are many variations such as orange, brown blue, red and blue. The fruits are capsules and are egg-shaped.

In the cosmetic composition for moisturizing the skin of the present invention, the moisturizing agent preferably includes at least one selected from cellular moisturizing and intercellular moisturizing. The cosmetic composition for moisturizing the skin comprising the hydrolyzed malt extract, the hydrolyzed gardenia extract, and the hydrolyzed tricolor violet extract according to the present invention in one embodiment is effective for moisturizing skin cells and between cells by confirming the expression of Aquaporin-3 and Claudin-1. It was confirmed that an excellent effect was shown. In addition, it showed an excellent effect on water retention ability.

Therefore, it can be provided in various formulations having an excellent skin moisturizing effect by using a cosmetic composition containing a hydrolyzed extract of malt, gardenia Florida , and Viola Tricolor as an active ingredient.

On the other hand, aquaporin (AQP) is a type of membrane protein responsible for the transport of water in the cell membrane and is known as a water channel that selectively regulates the entry and exit of water molecules into and out of the cell while interfering with the movement of ions and solutes. There are 13 AQPs (AQP-0 to AQP-12) in mammals, with AQP-1, 2, 4, 5 and 8 primarily transporting water selectively, and AQP-3, 7, 9, and 10 transporting water as well as It is known that it functions to transport glycerol and other small solutes. In particular, it has been reported that AQP-3 is expressed in keratinocytes of the basal layer of the epidermis, and activation of AQP-3 in keratinocytes can be expected to deliver moisture deep into the skin, resulting in excellent skin moisturizing effect.

On the other hand, the moisturizing ability in the skin has a close relationship with the function of the skin barrier, and the skin barrier function may be destroyed by aging and external stimuli. Damage to the skin barrier causes a direct decrease in skin moisture content and wrinkle formation, and many studies are being conducted to solve the problem. Among them, the tight junction (TJ) between keratinocytes, the main cells constituting the layer, is acting as an important factor.

Tight junctions link cell-to-cell adhesion to prevent water loss inside the human body and intrusion of external harmful substances. As tight junction proteins, Occludin, Claudin, ZO-1, etc. are known, and most of the tight junction proteins exist between the granular cell layers of the epidermis. Among them, claudin-1, a transmembrane protein, has a ring-shaped structure and effectively blocks the movement of water by filling the space between cells and controlling the intercellular space. Recently, through research results, it was reported that Claudin-1 plays an important role in the skin barrier function, which affects moisture between skin cells.

In the cosmetic composition for moisturizing the skin of the present invention, the extraction may be, for example, extraction through any one extraction method selected from a solvent extraction method, a supercritical extraction method, and an ultrasonic extraction method. In this case, the solvent extraction method is, for example, water, anhydrous or hydrous lower alcohol having 1-4 carbon atoms, propylene glycol, butylene glycol, glycerin, acetone, ethyl acetate, chloroform, butyl acetate, diethyl ether, dichloromethane, hexane or Any one extraction solvent selected from the group consisting of mixtures thereof may be used.

In particular, in one embodiment of the present invention, the hydrolyzed malt extract, the hydrolyzed Gardenia Florida extract, the hydrolyzed Viola Tricolor extract, and the excellent moisturizing effect of each and a mixture thereof were confirmed, and the extract was When mixed in the same ratio, it was confirmed that a very good moisturizing effect was exhibited.

On the other hand, in the present invention, the mixed extract is preferably contained in an amount of 0.0001 to 30.0% by weight based on the total weight of the cosmetic composition. More preferably, it is good to contain 0.01 to 10% by weight based on the total weight of the cosmetic composition. When the content of the mixed extract is less than 0.0001% by weight, the skin moisturizing effect is insignificant, and when it exceeds 30.0% by weight, the distinct effect does not increase according to the content increase.

On the other hand, the components included in the cosmetic composition of the present invention may include components commonly used in cosmetic compositions in addition to the mixed extract of the present invention as an active ingredient, for example, antioxidants, stabilizers, solubilizers, vitamins, pigments and fragrances. It includes conventional adjuvants such as, and carriers.

The cosmetic composition of the present invention may be prepared in any formulation conventionally prepared in the art, for example, solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing , oil, powder foundation, emulsion foundation, wax foundation, pack, massage cream, spray, etc., but is not limited thereto. More specifically, it may be prepared in the form of a flexible lotion, a nourishing lotion, a nourishing cream, a massage cream, an essence, an eye cream, a cleansing cream, a cleansing foam, a cleansing water, a pack, a spray, or a powder.

When the formulation of the cosmetic composition of the present invention is a paste, cream or gel, animal oil, vegetable oil, wax, paraffin, starch, tracanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide, etc. This can be used.

When the formulation of the cosmetic composition of the present invention is a solution or emulsion, a solvent, solubilizer or emulsifier is used as a carrier component, for example, water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene fatty acid esters of glycol, 1,3-butylglycol oil, glycerol fatty esters, polyethylene glycol or sorbitan.

When the formulation of the cosmetic composition of the present invention is a suspension, as a carrier component, a liquid diluent such as water, ethanol or propylene glycol, ethoxylated isostearyl alcohol, a suspending agent such as polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester , microcrystalline cellulose, aluminum metahydroxide, bentonite, agar or tracanth may be used.

When the formulation of the cosmetic composition of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component, and in particular, in the case of a spray, additional chlorofluorohydro It may contain a propellant such as carbon, propane/butane or dimethyl ether.

When the formulation of the cosmetic composition of the present invention is cleansing containing surfactant, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyl taurate, sarcosinate, Fatty acid amide ether sulfate, alkylamidobetaine, fatty alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, lanolin derivative or ethoxylated glycerol fatty acid ester and the like can be used.

When the cosmetic composition of the present invention is a soap, a surfactant-containing cleansing formulation, or a surfactant-free cleansing formulation, it may be applied to the skin and then wiped off, removed, or washed off with water. As a specific example, the soap is liquid soap, powder soap, solid soap, and oil soap, the surfactant-containing cleansing formulation is a cleansing foam, cleansing water, cleansing towel, and cleansing pack, and the surfactant-free cleansing formulation is a cleansing cream , cleansing lotion, cleansing water, and cleansing gel, but not limited thereto.

Hereinafter, the configuration of the present invention will be described in detail through the following examples and experimental examples. However, the scope of the present invention is not limited only to the following examples and experimental examples, and includes modifications of technical ideas equivalent thereto.

[Preparation Example 1: Preparation of hydrolyzed malt extract]

100 g of malt was ground, 1,000 g of purified water was added, and hot water was extracted at 100° C. for 3 hours, followed by filtration with a 400 mesh filter cloth. After that, it was concentrated under reduced pressure to prepare a 'malt extract'.

Thereafter, 500 g of 1M HCl solution was added to 5 g of malt extract, followed by stirring at a temperature of 25° C. for 3 hours, followed by neutralization with 1 M NaOH solution to prepare 'hydrolyzed malt extract'.

[Preparation Example 2: Hydrolyzed gardenia ( Gardenia Florida ) Preparation of extract]

100 g of gardenia ( Gardenia Florida ) was crushed, 1,000 g of purified water was added, and hot water was extracted at 100° C. for 3 hours, followed by filtration with a 400 mesh filter cloth. Thereafter, it was concentrated under reduced pressure to prepare a 'Gardenia extract'.

Thereafter, 500 g of a 1M HCl solution was added to 5 g of the gardenia extract, followed by stirring at a temperature of 25° C. for 3 hours, followed by neutralization with a 1M NaOH solution to prepare a 'hydrolyzed gardenia extract'.

[Preparation Example 3: Hydrolyzed tricolor violet ( Viola Tricolor ) Preparation of extract]

100 g of Viola Tricolor was ground, 1,000 g of purified water was added, and hot water was extracted at 100° C. for 3 hours, followed by filtration with a 400 mesh filter cloth. After that, it was concentrated under reduced pressure to prepare a 'tricolor violet extract'.

Thereafter, 500 g of 1M HCl solution was added to 5 g of Tricolor violet extract, stirred at 25° C. for 3 hours, and neutralized with 1M NaOH solution to prepare 'hydrolyzed Tricolor violet extract'.

[Example 1 and Comparative Examples 1 to 6: Hydrolyzed malt extract, hydrolyzed gardenia ( Gardenia Florida ) extract and hydrolysis of tricolor violet ( Viola Tricolor ) preparation of extract mixture]

The mixture of the hydrolyzed malt extract, the hydrolyzed Gardenia Florida extract and the hydrolyzed Viola Tricolor extract prepared in Preparation Examples 1 to 3 was mixed in the composition ratio of Table 1 below, and DMSO It was dissolved in a concentration of 100 mg/ml and used in the following experiment.

ingredient Example 1 Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 Comparative Example 5 Comparative Example 6 Hydrolyzed Malt Extract One 3 - - 1.5 1.5 - Hydrolyzed Gardenia Extract One - 3 - 1.5 - 1.5 Hydrolyzed Tricolor Violet Extract One - - 3 - 1.5 1.5

[Experimental Example 1: Measurement of cell viability]

In this experiment, the toxicity of the extracts of Comparative Examples 1 to 3 or the mixture of Comparative Examples 4 to 6 and Example 1 on cells was measured. For this, cell viability was measured using MTT (3-(4,5-dimethylthiazol-2-yl)-2-5-diphenyl tetrazolium bromide) reagent.

Specifically, human keratinocytes (HaCaT, keratinocytes) were aliquoted in a 96-well plate at ^{a concentration of 2×10 4} cells/well, and cultured at 37° C., 5% CO ₂ condition for 24 hours. The culture medium was removed, and the extracts of Comparative Examples 1 to 3 or the mixtures of Comparative Examples 4 to 6 and Example 1 were serially diluted by concentration (1, 3, 10, 30 and 100 μg/ml) and cultured for 24 hours. did

Then, 20 μl of MTT reagent dissolved at 5 mg/ml was added, _{and reacted at 37° C., 5% CO 2} conditions for 2 hours. After the medium was completely removed, 100 μl of DMSO was added to the generated formazan to completely dissolve, and absorbance was measured at 540 nm.

As a result of the experiment, the mixture of Example 1 and the extracts of Comparative Examples 1 to 3 or the mixture of Comparative Examples 4 to 6 did not show cytotoxicity up to a concentration of 100 μg/ml ( FIG. 1 ). 1 is a result of measuring the toxicity of the extracts of Comparative Examples 1 to 3 or a mixture of Comparative Examples 4 to 6 and Example 1 on cells.

[Experimental Example 2: Confirmation of cell moisturizing effect through confirmation of expression of Aquaporin-3]

In order to evaluate the moisturizing effect at the cellular level, the effect of the extracts of Comparative Examples 1 to 3 or the mixture of Comparative Examples 4 to 6 and Example 1 on AQP-3, a channel involved in intracellular water absorption, was confirmed. As a positive control, dexamethasone was used.

In addition, when HaCaT cells were dispensed in a 6-well plate and the cells were filled to about 80%, the medium was treated with the extracts of Comparative Examples 1 to 3 or the mixture of Comparative Examples 4 to 6 and Example 1 at a concentration of 100 μg/ml. . Thereafter, after culturing for 24 hours, cells were collected, and proteins were isolated using 1× cell lysis buffer.

Then, the protein was quantified by BCA assay and a certain amount of protein was electrophoresed on a 10% SDS-PAGE gel. Thereafter, it was transferred to a PVDF membrane and blocked for 1 hour using 5% skim milk. After reacting the primary antibody (AQP-3, Santa cruz , USA) at 4°C overnight (over night), the HRP-conjugated secondary antibody was reacted at room temperature for 2 hours. After that, it was washed with TBS/T and the band was confirmed by Chemi Doc instrument using the ECL reaction kit. Quantification of each band was quantified based on the expression of beta-actin (β-actin) to calculate the expression level of AQP-3.

As a result of the experiment, dexamethasone, a positive control, increased AQP-3 expression by about 73.0%, and the mixture of Example 1 increased AQP-3 expression by about 113.2% at a concentration of 100 μg/ml. The extract of Comparative Example 2 was 62.1%, the extract of Comparative Example 3 was 78.4%, the mixture of Comparative Example 4 was 27.2%, the mixture of Comparative Example 5 was 61.5%, the mixture of Comparative Example 6 was 59.8% of AQP-3 expression increased. In the other extracts of Comparative Example 1, there was no significant increase in AQP-3 expression. According to the above results, it was confirmed that the mixture of Example 1 was most effective for moisturizing the cells ( FIG. 2 ). 2 is a result of confirming the cell moisturizing effect through the control of Aquaporin-3 expression of the extracts of Comparative Examples 1 to 3 or the mixture of Comparative Examples 4 to 6 and Example 1.

[Experimental Example 3: Confirmation of intercellular moisturizing effect through confirmation of Claudin-1 expression]

The intercellular moisturizing effect of the extracts of Comparative Examples 1 to 3 or the mixture of Comparative Examples 4 to 6 and Example 1 on Claudin-1 involved in moisture loss inside the skin was measured as follows.

HaCaT cells were dispensed in a 6-well plate, and when the cells were about 80% full, the extract of Comparative Examples 1 to 3 or a mixture of Comparative Examples 4 to 6 and Example 1 was treated at a concentration of 100 μg/ml in the medium. Thereafter, after culturing for 24 hours, cells were collected, and proteins were isolated using 1× cell lysis buffer. Then, the protein was quantified by BCA assay and a certain amount of protein was electrophoresed on a 10% SDS-PAGE gel. Thereafter, it was transferred to a PVDF membrane and blocked for 1 hour using 5% skim milk. After reacting the primary antibody (Claudin-1, cell signaling , USA) at 4°C overnight (over night), the HRP-conjugated secondary antibody was reacted at room temperature for 2 hours. After that, it was washed with TBS/T and the band was confirmed by Chemi Doc instrument using the ECL reaction kit. Quantification of each band was quantified based on the expression of beta-actin (β-actin) to calculate the expression level of claudin-1.

As a result of the experiment, the mixture of Example 1 increased the expression of about 112.5% Claudin-1 at a concentration of 100 μg/ml. The extract of Comparative Example 1 was 62.2%, the extract of Comparative Example 2 was 13.4%, the mixture of Comparative Example 4 was 61.3%, the mixture of Comparative Example 5 was 67.8%, the mixture of Comparative Example 6 was 22.9% Claudin-1 increased the expression of In the case of the extract of Comparative Example 3, there was no significant increase in claudin-1 expression. Therefore, it was confirmed that the mixture of Example 1 was most effective for moisturizing between skin cells ( FIG. 3 ). 3 is a result confirming the moisturizing effect between cells through control of Claudin-1 expression of the extracts of Comparative Examples 1 to 3 or the mixture of Comparative Examples 4 to 6 and Example 1.

[Experimental Example 4: Confirmation of Water Holding Ability]

In this experiment, the water holding capacity of the extracts of Comparative Examples 1 to 3 or the mixture of Comparative Examples 4 to 6 and Example 1 was measured. As a comparative sample, a solution in which hyaluronic acid (HA) was added to water was used.

To this end, the experiment was carried out at a constant temperature, and 20 ml of each of the extracts of Comparative Examples 1 to 3 or the mixture of Comparative Examples 4 to 6 and Example 1 were put in a Petri dish, and each time (0, 2 , after 4, 6, 12 and 24 hours) were measured with a precision scale and recorded in Table 2 below.

Time
(hour) Vehicle
(HA) Example 1 Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 Comparative Example 5 Comparative Example 6 0 21934.0 23562.1 24401.3 23468.7 22993.2 21395.7 22822.6 23250.2 2 21276.0 23185.1 23766.9 22905.5 22372.4 20903.6 22297.7 22785.2 4 20508.3 22737.4 23010.4 22318.8 21590.6 20368.7 21658.7 22227.2 6 19301.9 21936.3 21668.3 21121.9 20394.9 19363.1 20563.2 21181.0 12 17108.5 20876.0 19789.4 19526.0 18440.5 17972.3 19011.3 20041.7 24 13379.7 18402.0 16031.6 16287.3 14991.5 15233.7 15930.2 16972.7

Experimental results, Example 1 (21.9%), Comparative Example 6 (27.0%), Comparative Example 4 (28.8%), Comparative Example 5 (30.2%), Comparative Example 2 (30.6%), Comparative Example 1 (34.3%) , Comparative Example 3 (34.8%), water (HA) (39.0%) was found to have a small moisture reduction in the order.

That is, when compared with the extracts of Comparative Examples 1 to 3 or the mixture of Comparative Examples 4 to 6, it was confirmed that the mixture of Example 1 had excellent water binding force and thus the amount of evaporated moisture was the smallest (Fig. 4). ). 4 is a result showing the moisture reduction rate of the extracts of Comparative Examples 1 to 3 or the mixture of Comparative Examples 4 to 6 and Example 1.

[Formulation Example 1: Preparation of cosmetic composition]

A cosmetic composition containing 0.1 wt% of each of the hydrolyzed malt extract, the hydrolyzed Gardenia Florida extract, and the hydrolyzed Viola Tricolor extract, which is the mixture of Example 1, was prepared as shown in Table 3 below. This was called Formulation Example 1, and Comparative Formulation Example 1 was prepared as a control.

ingredient Formulation Example 1
(weight%) Comparative Formulation Example 1
(weight%) Hydrolyzed Malt Extract 0.1 - Hydrolyzed Gardenia Extract 0.1 - Hydrolyzed Tricolor Violet Extract 0.1 - 1,3-BG 10.0 10.0 glycerin 5.1 5.1 propylene glycol 4.2 4.2 tocopheryl acetate 3.0 3.0 liquid paraffin 4.6 4.6 triethanolamine 1.0 1.0 squalane 3.1 3.1 Macadamia Nut Oil 2.5 2.5 polysorbate 1.6 1.6 Sorbitan sesquirolate 1.6 1.6 Propylparaben 0.6 0.6 carboxyl vinyl polymer 1.5 1.5 incense a very small amount a very small amount antiseptic a very small amount a very small amount Purified water remaining amount remaining amount total 100.0 100.0

[Experimental Example 5: Measurement of skin moisture content]

In this experiment, it was attempted to confirm the skin moisture content of Formulation Example 1. To this end, the skin moisturizing power was evaluated by measuring the skin moisture content. Moisturizing power measurement by a skin moisture meter is a device that measures the moisture content of the skin surface (eg, stratum corneum) by means of capacitance measurement. , it has the advantage of maintaining a constant measurement level at a depth of 30-40 μm below the probe sensor contact area. The unit of measurement is displayed in an arbitrary unit (Arbitary unit, A.U.) assigned by the device.

In addition, the initial value was measured before application of the sample using a skin moisture meter (corneometer) under constant temperature and humidity conditions maintained at room temperature of 20 to 25° C. and relative humidity of 40 to 55%, and Formulation Example 1 and Comparative Formulation Example 1 were applied. did Thereafter, the skin moisture content was measured using a skin moisture meter for each time (after 10, 30, 60, 90, and 180 minutes), and the results are shown in Table 4.

(Unit: A.U.) 0 min 10 min 30 min 60 min 90 min 180 min Formulation Example 1 35.5 46.5 45.8 43.5 40.4 38.1 Comparative Formulation Example 1 35.9 40.7 38.4 36.4 35.4 36.1

As a result of the experiment, it was confirmed that Formulation Example 1 significantly increased skin moisture content compared to Comparative Formulation Example 1 ( FIG. 5 ). 5 is a result of measuring the skin moisture content when applying Formulation Example 1 and Comparative Formulation Example 1.

[Experimental Example 6: Measurement of transdermal water loss]

In this experiment, the amount of trans epidermal water loss (TEWL, trans epidermal water loss) of Formulation Example 1 was to be confirmed. The amount of transdermal water loss means that moisture inside the skin is evaporated when the barrier function of the skin is lost. The evaluation of water evaporation is expressed as g/h/m ² that evaporates per unit area per hour.

After using a tape to artificially remove dead skin cells from the skin by strapping the skin on the inside of the forearm, Formulation Example 1 and Comparative Formulation Example 1 were applied. Thereafter, the amount of evaporation of water in the skin was measured by time (after 10, 30, 60, 90 and 180 minutes) using a transdermal water loss meter (Tewameter TM 210, Courage and Khazaka, Germany), and the results are shown in Table 5 it was

(Unit: g/m ² /h) 0 min 10 min 30 min 60 min 90 min 180 min Formulation Example 1 10.4 20.7 12.4 11.1 10.8 10.1 Comparative Formulation Example 1 10.9 21.4 18.4 16.5 13.4 12.1

As a result of the experiment, it was confirmed that Formulation Example 1 had a very low transdermal water loss compared to Comparative Formulation Example 1 ( FIG. 6 ). 6 is a result of measuring the amount of transdermal water loss when applying Formulation Example 1 and Comparative Formulation Example 1.

Claims (4)

Hydrolyzed malt extract, hydrolyzed Gardenia Florida extract, and hydrolyzed Viola Tricolor extract are contained in equal proportions,
The hydrolyzed extract is a cosmetic composition for moisturizing the skin, characterized in that malt, gardenia, and tricolor violet are each extracted with hot water and then acid hydrolyzed.
According to claim 1,
The moisturizing is
A cosmetic composition for skin moisturizing, comprising at least one selected from cell moisturizing and intercellular moisturizing.
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