KR101987167B1 - Cosmetic composition for improving skin hydration and skin barrier function - Google Patents

Abstract

The present invention relates to a cosmetic composition for skin moisturizing and skin barrier strengthening. The present invention contains 0.1 to 10 wt% of complex extract of hydrolase-treated corncob, malt and flaxseed as an active ingredient, relative to the total weight of the cosmetic composition. The cosmetic composition comprising the complex extract of hydrolase-treated corncob, malt and flaxseed as an active ingredient is safe by containing a natural extract, the synergistic effect of which makes excellent skin moisturizing and skin barrier strengthening effect.

Description

Cosmetic composition for skin moisturizing and skin barrier strengthening {Cosmetic composition for improving skin hydration and skin barrier function}

The present invention relates to a cosmetic composition containing a hydrolytic enzyme-treated corncob ( Zea mays kernel), malt, flax seed complex extract as an active ingredient, specifically prepared by enzymatic decomposition The present invention relates to a cosmetic composition for skin moisturizing and skin barrier reinforcing, comprising a natural extract of corncob, malt and flaxseed as extracts.

The skin has the largest surface area among human body organs as well as the outermost area, protecting the body from various external shocks such as pathogenic bacteria, pollutants, ultraviolet rays, and heat, and excreting waste products and regulating moisture in the body. Keep your body temperature through. In addition, skin plays a very important role in cosmetic aspects. Having a healthy skin that is moist, shiny and elastic is what every woman wants and actually acts as a positive factor in terms of human self-esteem and life satisfaction. Healthy skin can be sustained by strengthening the skin barrier and increasing moisture. The stratum corneum of the epidermis, the outermost layer of the skin, acts as a barrier to the external harmful environment and plays an important role in preventing moisture evaporation of the skin. The stratum corneum has a thickness of about 0.06 ~ 1.00mm and contains about 10 ~ 20% moisture.When the moisture of the stratum corneum falls below 10%, the skin dries and becomes rough, abnormality occurs on the skin barrier and the amount of water loss to the outside Will increase. In particular, when the moisture of the stratum corneum is insufficient due to weather, temperature, humidity, cleaning habits, and the like, an abnormality occurs in the function of the skin barrier, thereby drying the skin and damaging skin health. Therefore, a healthy and beautiful skin can be maintained normally by supplying sufficient moisture to the stratum corneum, it is important to apply a sufficient moisturizer continuously for this purpose.

Current moisturizers widely used in cosmetics include glycerol, hyaluronic acid, silicone oil, and the like. Glycerol has three hydroxyl groups in one molecule, so it has high affinity with water molecules. Therefore, it not only prevents the moisture of the cosmetic itself from evaporating but also serves to hydrate and sustain the skin. However, there is a report that can cause skin dehydration, allergies, skin irritation, so be careful. Hyaluronic acid is a component that can hold a large amount of water and is also present in human skin, and is widely used as a moisturizing material for skin-friendly cosmetics. However, since hyaluronic acid is expensive, it is difficult to apply the effective amount to the product. In addition, silicone oil is a type of hermetic moisturizer that has a feature of forming a film on the skin without stickiness, but recently, there is a growing demand for silicone products without the negative recognition of synthetic oil among consumers. Therefore, there is a need for the development of an economical and safe natural moisturizer that can provide moisture to the skin and improve the skin barrier.

Corn ( Zea mays ), malt (malt) and flax seed (research) is actively researched not only as a functional food material but also as a highly functional natural cosmetic material. In this patent, to use the corn kernels (kernel), the skin-related patents of corn cob as Republic of Korea Patent Publication No. 10-2016-0036202 (Invention name: sunscreen cosmetics containing corn cob extract as an active ingredient Compositions) are disclosed. In addition, as a patent related to malt, Korean Patent Publication No. 10-1998-0087818 (name of the invention: a method of using malt syrup to remove sebum), Korean Patent Publication No. 10-2002-0077616 (name of the invention: malt ( External malt using malt), etc., and the results of human tests on skin external agent for whitening, improving atopy, and alleviating skin irritation using malt as active ingredients, but the effect of malt on skin moisturizing and skin barrier No details are mentioned. In addition, the Republic of Korea Patent Publication No. 10-2004-0059270 (name of the invention: a method for producing secoisolarysirinol and a cosmetic composition containing the same as an active ingredient) in the flaxseed extract is subjected to acid, alkali or enzyme reaction skin absorption rate A method for producing secoisolarysirinol having high anti-oxidant efficacy is disclosed, and Korean Patent No. 10-1440844 (name of the invention: cosmetic composition containing flaxseed mucus or mucus extract) contains mucus or mucus extract of flaxseed. The cosmetics for skin moisturizing are disclosed.

The present inventors conducted research to discover complex extracts derived from natural materials that enhance skin moisturizing power and strengthen skin barrier, and finally selected three materials of corncob, malt and flaxseed through screening. The present invention was completed by confirming that the hydrolytic enzyme treatment greatly enhanced the expression or activity of skin moisturizing and skin barrier-related factors in the case of corncob, malt and flaxseed extracts.

An object of the present invention created to solve the above problems, to provide a cosmetic composition for enhancing skin moisturizing and skin barrier reinforcing containing a hydrolase treatment corn cob, malt and flaxseed complex extract as an active ingredient.

The object of the present invention can be achieved by a cosmetic composition for skin moisturizing and skin barrier reinforcing containing 0.1 to 10% by weight relative to the total weight of the cosmetic composition as the active ingredient hydrolyzed corncob, malt and flaxseed composite extract. have.

Preferably, the hydrolase is characterized in that the β-glucosidase.

Preferably, the enzyme extract of corncob, malt and flaxseed of the present invention is prepared by hydrolyzing a mixed extract of corncob, malt and flaxseed obtained by using a shell dissolving solution as β-glucosidase. It is done.

Preferably, the hydrolase treated corncob, malt and flaxseed mixed extract of the present invention is characterized in that the corncob, malt and flaxseed are prepared by mixing at a weight ratio of 1 to 3: 1 to 3: 1 to 3, respectively. It is done.

Preferably, the hydrolase treated corncob, malt and flaxseed mixed extract of the present invention is characterized in that it is prepared by mixing in a weight ratio of 1: 3: 2.

Preferably, the shell solution of the present invention is the step of baking the shell at 1,000 ~ 1,500 ℃ 10-30 minutes; Grinding to 100-2,000 mesh size; Adding shell shell powder to purified water and heating at 100 to 120 ° C. for 5 to 30 minutes; It is prepared by a manufacturing method comprising the step of separating the powder with a filter, characterized in that the pH of 8 to 10.

Preferably, the present invention is palmitoyl glycine (Palmitoyl Glycine), ectoin (Ectoin), sodium palmitoyl proline (Sodium Palmitoyl Proline), Nymphaea Alba Flower Extract (Nymphaea Alba Flower Extract) as an active ingredient to the total weight of the cosmetic composition It is characterized by being prepared by mixing with respect to the weight percent of 0.001 to 10, respectively.

Preferably, the cosmetic composition of the present invention is a lotion, cream, essence, cleansing foam, cleansing water, pack, body lotion, body oil, body gel, shampoo, rinse, hair conditioner, hair gel, foundation, lipstick, mascara, It is characterized in that it is added to one of the formulations selected from the makeup base.

The present invention as described above is a cosmetic composition containing a hydrolase-treated corncob, malt and flaxseed complex extract as an active ingredient is safe to contain a natural extract, it has excellent skin moisturizing and skin barrier strengthening effect by its synergism .

Hereinafter, with reference to the embodiment of the present invention will be described.

Corn ( Zea mays ) is the world's three largest crops, known for its anti-diabetic, antibacterial, anti-oxidant and anti-aging effects. In addition to food crops, it is widely used in cosmetics, food and pharmaceutical materials. According to the corn-based studies, the content of cyanidin 3-O-glucoside, an anthocyanin, was higher than that of leaves, stems, stamens, and husks. Corn cob is a non-edible by-product of corn, and its production is high, but its utilization is low. Therefore, corn cobs can create new added value by increasing industrial utilization through development of cosmetic raw materials.

Malt is a malt that is produced by germinating and dried in wheat, barley, etc. at appropriate moisture, temperature, and oxygen conditions to produce a large amount of amylase, a starch, also called malt. Since ancient times, malt has been used mainly in the manufacture of alcohol and sorghum, and has been widely used in the production of syrups and extinguishing agents. In addition to starch degrading enzymes, malt contains phenols, ergosterol, amino acids, hordenine, gramine, and hydroxycinnamic acid. This is known.

Flax seed refers to the seeds of Linum usitatissimum seed, and has been reported as alpha-linolenic acid, lignans, and omega 3-fatty acids as main ingredients, as well as dietary fiber and protein. It is known to contain vitamins, folic acid, minerals and tocopherols. In other words, it contains a variety of components that exhibit excellent efficacy and is likely to be used as a material of high-functional products. In fact, the physiological activity of flaxseed is known to inhibit tumor growth, inhibit cholesterol, inhibit blood coagulation, and antioxidants.

In the present invention, corncob, malt and flaxseed are characterized in that the active ingredient is used to enhance the active ingredient. In addition, it is characterized by using the natural plant extract prepared by hydrolysis after extraction with a shell solution with a mixed extraction of the hydrolase-treated corncob, malt and flaxseed as an active ingredient.

First, corncob, malt and flaxseed are extracted with at least one solvent selected from the group consisting of water, lower alcohols having 1 to 4 carbon atoms, ethyl acetate, glycerin, ethylene glycol, propylene glycol and butylene glycol. Extracted corncob, malt and flaxseed may be mixed and extracted, or each extract may be prepared and mixed. Subsequently, the corncob, malt and flaxseed mixed extracts are treated with a hydrolase, preferably β-glucosidase, to obtain a hydrolyzate. At this time, the corncob, malt and flaxseed are each mixed in a weight ratio of 1 to 3: 1 to 3: 1 to 3, preferably in a weight ratio of 1: 3: 2 :.

After mixing in the above ratio to prepare a hydrolyzate, the active ingredient was strengthened to show more excellent skin improvement activity.

The mixed extract of corncob, malt and flaxseed treated with the hydrolase showed superior skin moisturizing activity compared to the mixed extract of corncob, malt and flaxseed.

According to an embodiment of the present invention, the enzyme extract of corncob, malt and flaxseed is prepared as follows.

First, a mixture of corncob, malt, and flaxseed is used to prepare shell extract using shell solution as an extractant. The shell solution is an alkaline solution containing the useful components of the eluted shell, according to one embodiment of the present invention is prepared by the following method. First, the shell is calcined to a high temperature to powder, and impregnated and heated the powder in purified water to prepare a strong alkali solution. More specifically, first, the shells of at least one shell selected from the group consisting of oysters, ramie shells and abalone are baked at 1,000 to 1500 ° C for 10 to 30 minutes, and then pulverized to 100 to 2,000 mesh sizes. The shell powder is placed in 10-20 times the volume of purified water and heated at 100-120 ° C. for 5-30 minutes. Filtration removes the solid powder to obtain the filtrate. The shell solution is used to exhibit a strong alkalinity of pH 8-10, more preferably pH 10.

The corncobs, malt and flaxseed mixed extract extracted with the shell lysate are treated with hydrolase, preferably β-glucosidase, to prepare an enzyme extract. At this time, the enzyme extracts of corncob, malt and flaxseed are prepared by mixing in a weight ratio of corncob, malt and flaxseed 1 to 3: 1 to 3: 1 to 3, respectively, in a weight ratio of 1: 3: 2. When prepared by mixing it showed the best skin moisturizing activity.

The mixed extract of the hydrolase-treated corncob, malt and flaxseed as an active ingredient is contained in an amount of 0.1 to 10% by weight based on the total weight of the cosmetic composition. At this time, the mixed extract of the hydrolase-treated corncob, malt and flaxseed is most preferably contained in a weight ratio of 1: 3: 2, respectively.

The hydrolase-treated corncob, malt, and flaxseed complex extracts of the present invention do not affect the survival rate of skin-derived cell lines (Test Example 1), and the expression or activity of factors related to skin moisturization and skin barrier function by synergy. Was increased (Test Examples 2-4). In addition, this composite extract showed formulation safety at room temperature, refrigeration and constant temperature when applied to serum and cream formulations (Test Example 5). Therefore, the cosmetic composition may be usefully used as a skin moisturizing or skin barrier reinforcing cosmetic.

In addition, the cosmetic composition of the present invention may be prepared by mixing palmitoylglycine, ectoin, sodium palmitoylproline, alba water lily flower extract as an effective ingredient in an amount of 0.001 to 10% by weight, respectively, based on the total weight of the cosmetic composition.

 The cosmetic composition of the present invention may be prepared in any of the formulations commonly prepared, such as lotion, cream, essence, cleansing foam, cleansing water, pack, body lotion, body oil, body gel, shampoo, rinse, hair Conditioners, hair gels, foundations, lipsticks, mascaras, makeup bases, and the like.

EXAMPLE

Hereinafter, examples will be described in detail to help understand the present invention. However, the following examples are merely to illustrate the content of the present invention is not limited to the scope of the present invention. The embodiments of the present invention are provided to more completely explain the present invention to those skilled in the art.

Preparation Example 1 Preparation of Corn Cob Extract

The corncob was extracted with three times the weight of 70% hydrous ethanol as the extraction solvent and heated to 80 ~ 100 ℃ in an extractor (Cosmos-660, Gyeongseo machine) equipped with a cooling condenser.

 After extraction by the above method, the mixture was left at room temperature for 3 days, and the precipitate was filtered through 300 mesh filter paper, and the precipitate was filtered twice with Edventech No. 5 filter paper and Whatman GFC 150 mm filter paper. After concentrating at a temperature of 40 ° C. ~ 50 ° C. using a decompression concentrator (Coolace CCA-1100, EYELA), using a spray dryer (B-290, BUCHI Co.), the inlet temperature is 180 ° C., an aspirator. Efficiency 100% (35 cm3 / hr), Pump efficiency 25% (7.5 ml / min), Nozzle Cleaner 4 and Rotameter: Dry under conditions of 30 mm (357 l / hr) 10 g of the title powder was obtained.

Preparation Example 2 Preparation of Malt Extract

5 times the weight of 70% hydrous ethanol as the extraction solvent in the malt and heated to 80 ℃ ~ 100 ℃ in an extractor (Cosmos-660, Gyeongseo machine) equipped with a cooling condenser and extracted three times each 2 hours.

 After extraction by the above method, the mixture was left at room temperature for 3 days, and the precipitate was filtered through 300 mesh filter paper, and the precipitate was filtered twice with Edventech No. 5 filter paper and Whatman GFC 150 mm filter paper. After concentrating at a temperature of 40 ° C. ~ 50 ° C. using a decompression concentrator (Coolace CCA-1100, EYELA), using a spray dryer (B-290, BUCHI Co., Ltd.), the inlet temperature is 180 ° C., an aspirator. Efficiency 100% (35 cm3 / hr), Pump efficiency 25% (7.5 ml / min), Nozzle Cleaner 4 and Rotameter: Dry under conditions of 30 mm (357 l / hr) 10 g of the title powder was obtained.

Preparation Example 3 Preparation of Flax Seed Extract

Flaxseed was extracted with 5 times the weight of 70% hydrous ethanol as the extraction solvent, and was heated to 80 ~ 100 ℃ in an extractor (Cosmos-660, Gyeongseo machine) equipped with a cooling condenser and extracted three times for 2 hours.

 After extraction by the above method, the mixture was left at room temperature for 3 days, and the precipitate was filtered through 300 mesh filter paper, and the precipitate was filtered twice with Edventech No. 5 filter paper and Whatman GFC 150 mm filter paper. After concentrating at a temperature of 40 ° C. ~ 50 ° C. using a decompression concentrator (Coolace CCA-1100, EYELA), using a spray dryer (B-290, BUCHI Co., Ltd.), the inlet temperature is 180 ° C., an aspirator. Efficiency 100% (35 cm3 / hr), Pump efficiency 25% (7.5 ml / min), Nozzle Cleaner 4 and Rotameter: Dry under conditions of 30 mm (357 l / hr) 10 g of the title powder was obtained.

Preparation Example 4-6 Preparation of Corncob, Malt and Flaxseed Mixture Extracts

After mixing corncob, malt and flaxseed at the weight ratio of Table 1, add 5 times the weight of 70% hydrous ethanol as the extraction solvent, and heat it to 80 ~ 100 ℃ in an extractor (Cosmos-660, Gyeongseo machine) equipped with a cooling condenser Extraction was performed 3 times in total for 2 hours. The mixture was heated to 3 times in total for 2 hours.

 After extraction by the above method, the mixture was left at room temperature for 3 days, and the precipitate was filtered through 300 mesh filter paper, and the precipitate was filtered twice with Edventech No. 5 filter paper and Whatman GFC 150 mm filter paper. After concentrating at a temperature of 40 ° C. ~ 50 ° C. using a decompression concentrator (Coolace CCA-1100, EYELA), using a spray dryer (B-290, BUCHI Co., Ltd.), the inlet temperature is 180 ° C., an aspirator. Efficiency 100% (35 cm3 / hr), Pump efficiency 25% (7.5 ml / min), Nozzle Cleaner 4 and Rotameter: Dry under conditions of 30 mm (357 l / hr) 10 g of the title powder was obtained.

Preparation Example 7: Preparation of Shell Powder and Solution

1 kg of oyster shells were calcined for 30 minutes with 1,200 ° C. direct and indirect heat, and the shells were pulverized with 200 mesh. The oyster shell powder was placed in 10-fold volume of purified water and heated for 30 minutes with heat of 100 ° C. The solid powder was separated by a filter to obtain a shell solution.

Preparation Example 8 ~ 10: Preparation of corncob, malt and flaxseed extract using shell solution

The extract extract prepared in Preparation Examples 4 to 6 was added to the shell dissolving solution (pH 10) of Preparation Example 7 in a volume of 10 times and heated at 80 to 100 ° C. in an extractor (Cosmos-660, Gyeongseo Machinery) for 2 hours. A total of three extractions were made. After the extraction by the above method, the precipitate obtained by standing at room temperature for 3 days was filtered through a 300 mesh filter paper, and the precipitate was filtered twice with an Adventech No. 5 filter paper and a Whatman GFC 150 mm filter paper. After concentrating at a temperature of 40-50 ° C. using a decompression concentrator (Coolace CCA-1100, EYELA), using a spray dryer (B-290, BUCHI Co., Ltd.), the inlet temperature is 180 ° C., aspirator efficiency. 100% (35 cm 3 / hour), Pump efficiency 25% (7.5 ml / min), Nozzle Cleaner 4 and Rotameter: Dry under conditions of 30 mm (357 liters / hour) A mixed extract was obtained.

Examples 1 to 3 Preparation of Corncob, Malt and Flaxseed Enzyme Extracts

500 ml of acetate buffer (acetate buffer, pH 4.0) and 25 ml of β-glucosidase enzyme were added to the extract prepared in Preparation Examples 8 to 10 and reacted at 55 ° C. at 100 rpm for 4 hours. Next, the reaction was concentrated in vacuo after heating to 90 ° C. for 5 minutes to complete the enzymatic reaction. The concentrate was dried at 60 ° C. for 30 hours. After extraction by the above method, the mixture was left at room temperature for 3 days, and the precipitate was filtered through 300 mesh filter paper, and the precipitate was filtered twice with Edventech No. 5 filter paper and Whatman GFC 150 mm filter paper. After concentrating at a temperature of 40 ° C. ~ 50 ° C. using a decompression concentrator (Coolace CCA-1100, EYELA), using a spray dryer (B-290, BUCHI Co., Ltd.), the inlet temperature is 180 ° C., an aspirator. Efficiency 100% (35 cm3 / hr), Pump efficiency 25% (7.5 ml / min), Nozzle Cleaner 4 and Flowmeter: Dried under conditions of 30 mm (357 l / hr) An enzyme extract was obtained.

Test Example 1: Confirmation of Cytotoxicity

To determine the cytotoxicity, the keratinocyte line HaCaT was incubated in IMDM medium containing 10% FBS and inoculated at a cell concentration of 5 X 10 ⁵ in 96 well plates for 24 hours in a 37 ° C 5% CO ₂ incubator. . After incubation, the medium was removed, and the extract prepared in Examples and Examples above was diluted with dimethyl sulfoxide so as to have a sample concentration of 0.1, 0.5, and 1.0%. [4,5-dimethylthiazol-2yl]-2,5-diphenyltetrazoliumboromide, Sigma, USA) solution was added to each well 100ml (3 mg / ml) and further incubated for 4 hours. Thereafter, the supernatant was removed, 150 μl of dimethyl sulfoxide was added, and the resulting formazan was dissolved for 30 minutes, and the absorbance was measured at 540 nm using a multimicroplate reader (Molecular device Spectra max190). Cell viability was calculated according to Equation 1 and the results are shown in Table 3 below.

As shown in the results of Table 3, all of the samples to which corncob, malt or flaxseed extract, and a mixed extract thereof were applied did not affect cytotoxicity as the cell survival rate was confirmed to be 95% or higher. It was confirmed that there was no problem.

Test Example 2 Confirmation of Increased Expression of Hyaluronic Acid Synthetase (HAS-3)

Human fibroblast NHDF was adjusted to 5 × 10 ⁵ cells / well using DMDM medium added with 10% FBS, inoculated into 12 well plates and incubated for 18 hours. After incubation, the samples were treated and incubated for 24 hours. RNA was extracted from the cultured cells, and the expression rate of hyaluronan synthase-3 (HAS-3) was confirmed by reverse transcription polymerase chain reaction (RT-PCR). The increase rate of HAS-3 expression was calculated by Equation 2, and the results are shown in Table 4. Hyaluronic acid was used as a control.

As shown in Table 4, the highest HAS-3 growth rate was shown in Example 3 containing the hydrolase-treated corncob, malt and flaxseed complex extracts.

Test Example 3: Confirmation of the inhibitory effect of hyaluronidase

 Hyaluronidase activity inhibitory effect was confirmed by applying the Morgan-Elson method as follows. The final enzyme activity of hyaluronidase was 400 NF unit / ml, the final concentration of HA was 0.4 mg / ml, and the compound 48/80 buffer solution (0.1 mg / ml) was used as an inactive hyaluronic acid. Hyaluronidase activity was measured based on the inhibitory effect of the activation step of nidase. The sample was dissolved in a buffer to prepare a sample solution, and the control group used a buffer solution instead of the sample solution. Hyaluronic acid was used as a control. The inhibition rate (%) of hyaluronidase activity was calculated by Equation 3 below, and the results are shown in Table 5.

A: Enzyme Activity of Wells Without Samples

B: Enzyme Activity of Well Added Samples

As shown in Table 5 above, Example 3 containing the hydrolase-treated corncob, malt and flaxseed complex extracts showed the best inhibitory effect of hyaluronidase activity compared to other preparations.

Test Example 4 Effect of Increased Gene Expression of Transglutaminase-1

Preparation Examples 1 to 10 and Examples 1 to 3 were then incubated in DMED / 10 FBS for 24 hours at 5% CO ₂ and 37 ° C. conditions. After 24 hours RNA isolation, cDNA synthesis and RT-PCR in cells were performed according to the method described by Komoroski et al. (Drug Metab. Dispos. 32: 512-518, 2004). The gene measured by RT-PCR confirmed the expression of transgulutaminase-1. GAPDH was used as a house keeping gene to identify the difference in gene expression.

The primer sequences of each gene are shown in Table 6. The extracted RNA was used as a template, and the total amount was 15 μl using an oligo dT-adaptor primer and DEPC water. After 10 minutes of denaturation at 70 ° C., reverse transcription of M-MLV RNA was carried out. CDNA was synthesized at 42 ° C. for 60 minutes and 70 ° C. for 15 minutes. The synthesized cDNA was amplified by a PCR reaction, and the total reaction solution was 25 μl. The final concentration of each reaction was 100 pmol primer, 1.0 μM, 0.2 mM dNTP mixture, 5 × green or colorless GoTaq Reaction buffer 1 × 1.5 mM, MgCl ₂ (PCR buffer) and 1.25 units of GoTaq DNA polymerase. PCR reaction conditions for denaturation, annealing and extension are as follows: 95 ° C. 2 minutes, 95 ° C. 30 seconds, 60 ° C. 30 seconds, 72 ° C. 1 minute, 72 ° C. 5 minutes, It was performed in 30-40 cycles. After the PCR reaction, 10 μl of the product was electrophoresed on a 2% agarose gel. Expression level was confirmed using Gel Documentation system (Korea Lab Tech, Cat.No DGS-200D).

The results calculated by Equation 4 are shown in Table 7, respectively.

As shown in Table 7 above, Example 3 containing the hydrolase-treated corncob, malt, and flaxseed complex extracts showed the best gene expression increasing effect of transglutaminase-1 compared to other preparations. .

Example 4 Preparation of Serum Containing Hydrolase Treated Corncob, Malt and Flaxseed Complex Extracts

Serum containing the mixture of Example 3 was prepared according to the conventional method in the composition and content of Table 8 below.

Example 5 Preparation of Cream Containing Hydrolase Treated Corncob, Malt and Flaxseed Complex Extracts

Cream containing the mixture of Example 3 was prepared according to the conventional method in the composition and content of Table 9 below.

Test Example 5: Confirmation of formulation stability

The formulations prepared in Examples 4 and 5 were placed in opaque glass containers in indoor, refrigerator and incubators maintained at room temperature (25 ° C.), refrigerated (4 ° C.) and constant temperature (50 ° C.) for 12 and 24 weeks. Storage and observation (discoloration, deodorization and separation) and stability was confirmed. The results are shown in Table 10.

<Formulation stability grade>

0: no change 1: slight change 2: change 3: extreme change

As shown in Table 10, it was confirmed that the formulations of Examples 4 and 5 were stable and did not exhibit discoloration discoloration and separation under 25 ° C, 4 ° C, and 0 ° C temperature conditions.

Claims (8)

Cosmetic composition for skin moisturizing and skin barrier reinforcing containing 0.1 to 10% by weight of the total enzyme composition of the hydrolyzed enzyme corncob, malt and flaxseed composite extract as an active ingredient.
The method of claim 1,
The hydrolase is β-glucosidase, characterized in that the skin moisturizing and skin barrier cosmetic composition for strengthening.
The method of claim 1,
The enzyme extract of corncob, malt and flaxseed is a skin moisturizing and skin barrier, characterized in that it is prepared by hydrolyzing a mixed extract of corncob, malt and flaxseed obtained by using the shell dissolving solution as β-glucosidase Reinforcement cosmetic composition.
The method of claim 1,
The hydrolase-treated corncob, malt and flaxseed mixed extract is prepared by mixing corncob, malt and flaxseed at a weight ratio of 1 to 3: 1 to 3: 1 to 3, respectively. Reinforcement cosmetic composition.
The method of claim 1,
The hydrolase-treated corncob, malt and flaxseed mixed extract is a skin composition for skin moisturizing and skin barrier reinforcing, characterized in that the mixture is prepared in a weight ratio of 1: 3: 2.
The method of claim 3, wherein
The shell melt solution is calcined for 10 to 30 minutes at 1,000 ~ 1500 ℃ shell; Grinding to 100-2000 mesh size; Adding shell shell powder to purified water and heating at 100 to 120 ° C. for 5 to 30 minutes; It is prepared by a manufacturing method comprising the step of separating the powder with a filter, cosmetic composition for skin moisturizing and skin barrier reinforcement, characterized in that pH 8 ~ 10.
The method of claim 1,
Skin moisturizing and skin barrier reinforcing cosmetics, which are manufactured by mixing palmitoylglycine, ectoin, sodium palmitoylproline, and alba water lily flower extract as an active ingredient in an amount of 0.001 to 10% by weight, based on the total weight of the cosmetic composition. Composition.
The method of claim 1,
The cosmetic composition is a formulation selected from a lotion, cream, essence, cleansing foam, cleansing water, pack, body lotion, body oil, body gel, shampoo, rinse, hair conditioner, hair gel, foundation, lipstick, mascara, makeup base Cosmetic composition for skin moisturizing and skin barrier reinforcement, which is added to.