JP5990089B2 - Composition comprising salted fermented ginseng extract - Google Patents

Description

  The present invention relates to a skin regeneration composition containing a salted and fermented ginseng extract.

  The skin acts as a protective film that protects the body from the outside. When such a skin is damaged, it is healed by the natural healing action of the living body.

  The wound healing process is roughly divided into four stages: an inflammatory stage, a re-epithelialization stage, a proliferative stage, and a mature stage. During the inflammatory phase, immune cells that have migrated through the blood vessels appear at the site of the wound. Subsequently, growth factors and signaling substances that induce the formation of granular tissue are secreted. In the absence of a serious infection, the inflammatory phase generally proceeds short.

  The proliferative phase becomes similar to the re-epithelializing phase, but exhibits the property that granular tissue is formed at the site of the wound. The granule tissue is composed of extracellular matrix components such as immature collagen, fibronectin or hyaluronic acid together with fibroblasts and inflammatory cells. It is an important key for wound healing that this granular tissue rapidly fills the wound site and has a structured structure. Thereafter, a new epidermis is generated and the epithelial layer is reconstructed while the surface of the detached wound is covered with a keratinocyte layer.

  When wound re-epithelialization is complete, a series of processes is progressed in which the wound area decreases through the increase and reorganization of connective tissue. During maturation, the cells and capillaries of the convalescent tissue gradually disappear, but if such tissue is hyperplastic or does not degrade normally, scars may form.

  It is important that wounds are healed not only quickly, but without side effects and scars, so for wound healing, inflammation is suppressed, growth factors are regulated, and rapid cellular Needs to be played.

  Recently, with the trend of pursuing well-being, there is a demand for a product utilizing the fermented product obtained by directly fermenting without adding an artificial product. However, such natural fermentation methods are often difficult to use in actual product production because of the risk of spoilage or contamination by Escherichia coli, anaerobic bacteria, and the like that are harmful to the human body. In order to solve this, a method of sterilization, such as boiling before fermentation, may be used, but in this case, since the active ingredient can be destroyed by heat, it does not include a sterilization process and is a safe fermentation Things need to be developed.

Specification of Korean Patent Publication No. 10-2010-0014100 (published on February 10, 2010)

  The present invention is intended to provide a composition excellent in skin regeneration and wound healing effects. The present invention is also intended to provide a method for producing the composition.

One aspect of the present invention provides a composition for skin regeneration comprising a salted and fermented ginseng extract as an active ingredient.
Another aspect of the present invention provides a method for producing a skin regenerating composition, which includes adding salt to a Korean ginger and fermenting it.

  The composition according to the present invention has an excellent skin regeneration, wound healing, and scar reduction effect by including a salted and fermented ginseng extract as an active ingredient. Moreover, since the said composition shows little cytotoxicity, the person who has sensitive skin can use it without hesitation.

FIG. 1 is a graph showing the cell regeneration effect of a salted and fermented ginseng extract. FIG. 2 is a graph showing the presence or absence of cytotoxicity of the salted and fermented ginseng extract.

  In the present specification, “skin” means a tissue covering the body surface of an animal, and is the broadest concept including not only the tissue covering the body surface such as the face or body but also the scalp and hair. is there. In the present specification, “sensitive skin” refers to skin that has low resistance to external stimuli and is prone to abnormal reactions such as irritation, hot flashes, burning, itching, flushing, dryness, inflammation, etc. due to external stimuli. Examples of the cause of such sensitive skin include innate skin characteristics, family history, medical history, misuse / abuse of cosmetics, living environment or stress. In the present specification, the “extract” is a broad concept including all substances obtained by extracting natural components regardless of the extraction method, the extraction solvent, the extracted components or the form of the extract. .

  Hereinafter, the present invention will be described in detail.

  One aspect of the present invention provides a composition for skin regeneration comprising a salted and fermented ginseng extract as an active ingredient. Another aspect of the present invention provides a method for producing a skin regenerating composition, which includes adding salt to a Korean ginger and fermenting it.

  Shiozo is one of the traditional food storage methods expressed as salting, and can be applied to both animal foods and vegetable foods. Specifically, salt storage is a method of preventing normal decay and contamination by adding salt to foods and assisting normal fermentation, and is mainly used for the production of kimchi, miso, salted vegetables and the like. In one aspect of the present invention, the salted fermentation includes sake brewed rice, kimchi, soy sauce, and brewed brewed rice, which is a fermentation method unique to Korea, used for the production of various salted vegetables.

  In one aspect of the present invention, a salted and fermented ginseng extract obtained by pickling and fermenting ginseng with salt has an excellent cell regeneration effect. Therefore, the composition containing this as an active ingredient can have an excellent skin regeneration effect, skin recovery effect, and wound healing and scar reduction effect. In addition, since the salted and fermented ginseng extract has almost no cytotoxicity, the composition containing it can be used freely by those with sensitive skin.

In one aspect of the present invention, Panax ginseng CA Mayer is a Korean cocoon that grows naturally in nature, a water jar that is an unprocessed ginseng, and the skin is peeled off. All the parts of Korean ginseng such as roots, leaves, berries, flowers or stems may be used, including all the white pods dried in the above or steamed steamed syrup.
In one aspect of the present invention, the salt used in the salt storage means a salty-tasting crystal body mainly composed of sodium chloride, and is a salt obtained by boiling seawater concentrated in a tidal flat, specifically, sun After passing seawater through dried tidal flat sand to increase the saltiness, boiled in a kettle and boiled in the traditional Korean salt, seawater is drawn into the salt field, and then moisture is evaporated by wind and sunlight You are allowed to make the solar salt, rock salt water from the lake that contained a lot of salt produced by mining a vaporized remaining salt salt forming a strata or rock is deposited is, by dissolving a raw salt Mg ²⁺ From purified salt (flavored salt) obtained by recrystallization after removing impurities such as Ca ²⁺ , and regenerated salt (flower salt) obtained by dissolving impurities after dissolving sun salt in water and heating again to crystallize One or more selected from the group consisting of, but not limited to. In another aspect of the present invention, the salt used for salting contains 0.1-10% (w / v), specifically 1-5% (w / v) salt water.

  In one aspect of the present invention, the fermentation may be performed using edible microorganisms, specifically mold, and more specifically koji. In another aspect of the present invention, the fermentation is performed at 18 to 28 ° C., specifically 22 to 24 ° C., 3 days to 6 months, specifically 4 days to 1 month, more specifically 5 days to It may be done for 10 days.

In one aspect of the present invention, the salted and fermented ginseng extract may be obtained by extracting the salted and fermented ginseng with water, an organic solvent, or a mixed solvent of water and an organic solvent. In the above, water includes purified water, and the organic solvent includes one or more selected from the group consisting of alcohol, glycerin, ethyl acetate, butylene glycol, propylene glycol, dichloromethane, and hexane. Selection In the above, the alcohol includes lower alcohols C ₁ -C _5, lower alcohols C ₁ -C ₅ include methanol, ethanol, isopropyl alcohol, n- propyl alcohol, from the group consisting of n- butanol and isobutanol Including one or more of, but not limited to. In one aspect of the invention, the extraction may be performed at 20-70 ° C., specifically 30-50 ° C., for 4-12 hours, specifically 5-10 hours.

  The composition according to one aspect of the present invention is based on the total weight of the composition, 0.01 wt% to 50 wt%, specifically 0.1 wt% to 30 wt%, more specifically 1 wt% to 10% by weight of salted and fermented ginseng extract may be included. When included in the above range, not only is it suitable for expressing the intended effect of the present invention, but also the stability and safety of the composition can be satisfied together, and in terms of cost effectiveness, the above range is also included. May be appropriate to use.

  A method for producing a composition for skin regeneration comprising a salted and fermented ginseng extract according to one aspect of the present invention, the step of aging the ginseng with salt before adding the salt to the ginseng and fermenting; The method may further include the step of steaming the aged Korean ginseng to produce red koji. The aging may be performed at 22 to 24 ° C. for 1 to 15 days, specifically 5 to 10 days.

  According to another aspect of the present invention, there is provided a method for producing a composition for skin regeneration comprising a salted and fermented ginseng extract. After the step of adding salt to the ginseng and fermenting the fermented ginseng with a solvent, An extraction step; after extraction with a solvent, a step of obtaining a liquid substance by immersion at room temperature, heating and filtration; and if necessary, evaporating the solvent of the liquid substance, or spray drying or freeze drying to form a powder. A manufacturing step may further be included.

  One aspect of the present invention provides a cosmetic composition comprising a salted and fermented ginseng extract as an active ingredient. The cosmetic composition may have a skin regeneration effect. Specifically, the cosmetic composition may heal wounds and reduce scars. In particular, since the cosmetic composition has low toxicity to skin cells, it can be used regardless of the type of skin of the user. The skin type includes sensitive skin or dry skin.

  The cosmetic composition according to the present invention may be provided in any dosage form suitable for topical application. For example, a solution, an emulsion obtained by dispersing an oil phase in an aqueous phase, an emulsion obtained by dispersing an aqueous phase in an oil phase, a suspension, a solid, a gel, a powder, a paste, a foam or an aerosol composition May be provided in various dosage forms. Such dosage form compositions may be prepared according to routine methods in the art.

  The cosmetic composition according to the present invention may contain, in addition to the substances described above, other components that can give a synergistic effect to the main effect, as long as the main effect is not impaired. Further, the cosmetic composition according to the present invention comprises a moisturizer, emollient, surfactant, ultraviolet absorber, preservative, bactericidal agent, antioxidant, pH adjuster, organic and inorganic pigments, fragrance, and cooling agent. Or it may further contain an antiperspirant. The blending amount of the components can be easily selected by those skilled in the art within a range not impairing the object and effect of the present invention, and the blending amount is 0.01 to 5% by weight based on the weight of the entire composition, Specifically, it may be 0.01 to 3% by weight.

  One aspect of the present invention provides a pharmaceutical composition comprising a salted and fermented ginseng extract as an active ingredient. The pharmaceutical composition may have a skin regeneration effect, specifically heal wounds and reduce scars.

  The pharmaceutical composition according to one aspect of the present invention may be administered orally, rectally, topically, transdermally, intravenously, intramuscularly, intraperitoneally, subcutaneously and the like.

  Dosage forms for oral administration may be, but are not limited to, tablets, pills, soft and hard capsules, granules, powders, fine granules, solutions, emulsions or pellets. Absent. In addition to the active ingredient, these preparations include diluents (eg lactose, dextrose, sucrose, mannitol, sorbitol, cellulose or glycine), lubricants (eg silica, talc, stearic acid or polyethylene glycol), or binders ( Examples: magnesium aluminum silicate, starch paste, gelatin, tragacanth, methylcellulose, sodium carboxymethylcellulose or polyvinylpyrrolidine). Depending on the case, you may contain pharmaceutical additives, such as a disintegrating agent, an absorber, a coloring agent, a flavoring agent, or a sweetening agent. The tablets may be manufactured by conventional mixing, granulating or coating methods.

  Formulations for parenteral administration may be, but are not limited to, injections, drops, lotions, ointments, gels, creams, suspensions, emulsions, suppositories, patches or sprays. .

  The active ingredient of the pharmaceutical composition according to one aspect of the present invention will vary depending on the age, sex, weight, pathological condition and severity of the subject to be administered, the route of administration or the judgment of the prescriber. The determination of the dosage based on these factors is within the level of those skilled in the art, and the daily dosage is, for example, 0.1 mg / kg / day to 100 mg / kg / day, more specifically 5 mg / kg / day. Although it may be set to -50 mg / kg / day, it is not limited to this.

  Hereinafter, the configuration and effects of the present invention will be specifically described with reference to examples and experimental examples. However, these examples and experimental examples are provided for illustrative purposes only to help understanding of the present invention, and the scope and scope of the present invention are not limited thereby.

[Examples 1-4] Manufacture of salted fermented red yeast rice extract 1 kg of Korean ginger and an appropriate amount of boiled salt are placed in a pottery so that the boiled salt covers the ginseng appropriately, and aged for 7 days. Then, after simple washing, steamed in a high-pressure steam sterilizer at 98 ° C. for 3 to 24 hours to produce red yeast rice. Furthermore, it is made to dry for 24 hours with a 60 degreeC warm air dryer. Thereafter, 10% by weight of strawberry and 2% salt (boiled salt) water to the extent that the red potato is covered are added to the dried red potato weight, and the fermentation proceeds for 4 to 5 days. Thereafter, after simple washing, 45 g of salted red yeast rice fermented extract was obtained using water or an organic solvent.

  A method substantially the same as the production method of Example 1 is used, but 55 g of salted red yeast rice fermented extract is obtained using sun salt instead of boiled salt, which is referred to as Example 2.

  Although substantially the same method as the production method of Example 1 is used, 40 g of salted red yeast rice fermented extract is obtained by using regenerated salt instead of boiled salt, and this is referred to as Example 3.

  Although substantially the same method as the production method of Example 1 is used, 40 g of salted red yeast rice fermented extract is obtained using purified salt instead of boiled salt, which is referred to as Example 4.

[Example 5] Cell regeneration evaluation In order to evaluate the cell regeneration effect of the salted and fermented ginseng extract, the following experiment was conducted. First, a 96-well plate was inoculated with HaCaT 1 × 10 ⁴ cells / well. The medium was replaced with a medium not added with FBS and cultured for 1 hour. Thereafter, the medium was removed, and the medium containing 1% FBS was subjected to 1, 5, 10, 50, and 100 ppm of Example 1, and 10, 50, and 100 ppm of Examples 2 to 4, respectively. The extract was dissolved and then processed. At this time, a medium containing 1% and 10% FBS was used as a positive control group. After culturing for 48 hours, WST-1 was treated with 100 μL / well. Thereafter, the cells were cultured at 37 ° C. in a 5% CO ₂ incubator for 3 hours. After culturing, the cell regeneration effect was confirmed by measuring the absorbance at 450 nm using a microplate reader. The results are shown in FIG.

  As can be seen from FIG. 1, the salted and fermented ginseng extracts of Examples 1 to 4 all exhibited cell regeneration effects. Among them, Example 1 which is a salted and fermented ginseng extract using boiled salt showed a significant and most excellent cell regeneration effect with respect to 1% FBS. Through this, the salted and fermented ginseng extract, especially the salted and fermented ginseng extract using boiled salt, promotes cell regeneration and has the effect of regenerating skin and healing wounds. Can do.

[Example 6] Cytotoxicity evaluation (MTT analysis)
In order to evaluate the cytotoxicity of the salted fermented ginseng extract, the following experiment was performed. First, a 96-well plate was inoculated with HaCaT 1 × 10 ⁴ cells / well. After culturing for 24 hours, the cells were further cultured for 24 hours in a medium not containing FBS. Thereafter, the medium was removed, 100 μl of PBS was added, and 40 mJ UVB was irradiated. After the salted and fermented red yeast rice extract of Example 1 was treated with 1, 10 and 100 ppm and cultured for 48 hours, 100 μl each of MTT solution (0.5 mg / mL) was added, and further incubated for 3 hours at 37 ° C. In culture. At this time, a control group that was not irradiated with UVB was also tested. When the solution was deleted, the formazan formed was dissolved in 100 μl of DMSO, and then the absorbance at 570 nm was measured to confirm cytotoxicity. The results are shown in FIG.

  As can be seen from FIG. 2, the salted and fermented red yeast rice extract has almost no cytotoxicity, so that it can also be used by persons with sensitive skin.

  Examples of dosage forms of compositions according to one aspect of the present invention are described below, but can be applied to various other dosage forms, which are not intended to limit the present invention. It is for trying to explain.

[Dosage Form Example 1] Lotion A lotion is produced according to the usual method according to the composition described in the table below.

[Dosage Form Example 2] Cream According to the usual method, a cream is produced according to the composition described in the table below.

[Formulation Example 3] Soft capsule 100 mg salted fermented ginseng extract, 50 mg soybean extract, 180 mg soybean oil, 50 mg red bean extract, 2 mg palm oil, 8 mg palm hardened oil, 4 mg yellow wax and 6 mg lecithin mixed Then, a soft capsule is produced according to a usual method.

[Formulation Example 4] Tablets Salted fermented ginseng extract 100 mg, soybean extract 50 mg, glucose 100 mg, red yeast rice extract 50 mg, starch 96 mg and magnesium stearate 4 mg were mixed, and ethanol was added to form granules. After drying, it is compressed into tablets.

Claims (10)

  A composition for skin regeneration comprising a salted and fermented ginseng extract as an active ingredient.   The composition of claim 1, wherein the Korean ginseng comprises red yeast rice.   The composition according to claim 1, wherein the salt comprises one or more selected from the group consisting of boiled salt, sun salt, rock salt, purified salt and regenerated salt.   The composition according to claim 1, comprising 0.01% to 50% by weight of salted and fermented ginseng extract based on the weight of the entire composition.   The composition of claim 1 for healing wounds and reducing wounds.   The composition according to any one of claims 1 to 5, which is a cosmetic composition.   The manufacturing method of the composition as described in any one of Claims 1-5 including the step of adding salt to a ginseng and fermenting.   The manufacturing method of Claim 7 with which the said salt contains 0.1-10% (w / v) salt water.   The production method according to claim 7, wherein the fermentation is performed at 18 ° C. to 28 ° C. for 3 days to 6 months. Before the fermenting step,
The method according to claim 7, further comprising: aging the ginseng with salt; and steaming the aged ginseng to produce red koji.