KR102308556B1 - 변경된 일산화탄소 탈수소효소(codh) 활성을 가지는 유전자 조작된 박테리아 - Google Patents

변경된 일산화탄소 탈수소효소(codh) 활성을 가지는 유전자 조작된 박테리아 Download PDF

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KR102308556B1
KR102308556B1 KR1020177005901A KR20177005901A KR102308556B1 KR 102308556 B1 KR102308556 B1 KR 102308556B1 KR 1020177005901 A KR1020177005901 A KR 1020177005901A KR 20177005901 A KR20177005901 A KR 20177005901A KR 102308556 B1 KR102308556 B1 KR 102308556B1
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KR20170036789A (ko
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마이클 쾨프케
펑민 류
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란자테크 뉴질랜드 리미티드
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/18—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic polyhydric
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C12P7/065—Ethanol, i.e. non-beverage with microorganisms other than yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/16—Butanols
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y102/00—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2)
    • C12Y102/07—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2) with an iron-sulfur protein as acceptor (1.2.7)
    • C12Y102/07004—Carbon-monoxide dehydrogenase (ferredoxin) (1.2.7.4)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

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  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
KR1020177005901A 2014-08-11 2015-06-29 변경된 일산화탄소 탈수소효소(codh) 활성을 가지는 유전자 조작된 박테리아 Active KR102308556B1 (ko)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US201462036107P 2014-08-11 2014-08-11
US201462036101P 2014-08-11 2014-08-11
US201462036104P 2014-08-11 2014-08-11
US62/036,101 2014-08-11
US62/036,104 2014-08-11
US62/036,107 2014-08-11
PCT/US2015/038395 WO2016025096A1 (en) 2014-08-11 2015-06-29 Genetically engineered bacterium with altered carbon monoxide dehydrogenase (codh) activity

Publications (2)

Publication Number Publication Date
KR20170036789A KR20170036789A (ko) 2017-04-03
KR102308556B1 true KR102308556B1 (ko) 2021-10-07

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KR1020177005901A Active KR102308556B1 (ko) 2014-08-11 2015-06-29 변경된 일산화탄소 탈수소효소(codh) 활성을 가지는 유전자 조작된 박테리아

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US (1) US9365873B2 (enExample)
EP (1) EP3180421B1 (enExample)
JP (1) JP6454000B2 (enExample)
KR (1) KR102308556B1 (enExample)
CN (1) CN107223152B (enExample)
CA (1) CA2956204C (enExample)
ES (1) ES2728726T3 (enExample)
MY (1) MY178182A (enExample)
PL (1) PL3180421T3 (enExample)
PT (1) PT3180421T (enExample)
WO (1) WO2016025096A1 (enExample)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20190233854A1 (en) * 2016-10-13 2019-08-01 Sekisui Chemical Co., Ltd. Obligately anaerobic acetic acid-producing microorganism, and recombinant microorganism
KR102397454B1 (ko) * 2020-07-24 2022-05-12 울산과학기술원 전자전달체에 대한 친화도 및 반응 속도가 우수한 일산화탄소 탈수소효소 및 그의 용도
KR102393114B1 (ko) * 2020-07-24 2022-05-02 울산과학기술원 산소 내성 및 효소 활성이 우수한 일산화탄소 탈수소효소 및 그의 용도
KR20250103852A (ko) * 2023-12-28 2025-07-08 울산과학기술원 대기 조건하에서 산소 내성 및 효소 활성이 우수한 일산화탄소 탈수소효소 및 그의 용도

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20100151543A1 (en) 2008-12-16 2010-06-17 Andrew Reeves Recombinant microorganisms having modified production of alcohols and acids
US20140206901A1 (en) 2012-01-31 2014-07-24 Lanza Tech New Zealand Limited Recombinant microorganisms and methods of use thereof

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5593886A (en) 1992-10-30 1997-01-14 Gaddy; James L. Clostridium stain which produces acetic acid from waste gases
UA72220C2 (uk) 1998-09-08 2005-02-15 Байоенджініерінг Рісорсиз, Інк. Незмішувана з водою суміш розчинник/співрозчинник для екстрагування оцтової кислоти, спосіб одержання оцтової кислоти (варіанти), спосіб анаеробного мікробного бродіння для одержання оцтової кислоти (варіанти), модифікований розчинник та спосіб його одержання
US7704723B2 (en) 2006-08-31 2010-04-27 The Board Of Regents For Oklahoma State University Isolation and characterization of novel clostridial species
CA2703622C (en) 2007-11-13 2014-12-16 Lanzatech New Zealand Limited Clostridium autoethanogenum strain and methods of use thereof to produce ethanol and acetate
WO2010071697A1 (en) * 2008-12-16 2010-06-24 Genomatica, Inc. Microorganisms and methods for conversion of syngas and other carbon sources to useful products
CA2802015C (en) * 2010-06-09 2018-01-16 Coskata, Inc. Cloning and expression of the genes encoding key clostridial catalyzing mechanisms for syngas to ethanol production and functional characterization thereof
KR101865779B1 (ko) * 2011-11-16 2018-06-12 한국과학기술원 이산화탄소 고정능을 가지는 재조합 미생물 및 이를 이용한 유용물질의 제조 방법

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20100151543A1 (en) 2008-12-16 2010-06-17 Andrew Reeves Recombinant microorganisms having modified production of alcohols and acids
US20140206901A1 (en) 2012-01-31 2014-07-24 Lanza Tech New Zealand Limited Recombinant microorganisms and methods of use thereof

Also Published As

Publication number Publication date
JP2017522898A (ja) 2017-08-17
PT3180421T (pt) 2019-06-07
EP3180421A4 (en) 2018-02-28
EP3180421A1 (en) 2017-06-21
PL3180421T3 (pl) 2019-09-30
CA2956204C (en) 2018-11-27
EP3180421B1 (en) 2019-03-27
US20160040193A1 (en) 2016-02-11
WO2016025096A1 (en) 2016-02-18
CA2956204A1 (en) 2016-02-18
KR20170036789A (ko) 2017-04-03
CN107223152B (zh) 2019-03-01
MY178182A (en) 2020-10-06
CN107223152A (zh) 2017-09-29
ES2728726T3 (es) 2019-10-28
US9365873B2 (en) 2016-06-14
JP6454000B2 (ja) 2019-01-16

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