KR102214419B1 - 벌집형 반응튜브 - Google Patents
벌집형 반응튜브 Download PDFInfo
- Publication number
- KR102214419B1 KR102214419B1 KR1020157010847A KR20157010847A KR102214419B1 KR 102214419 B1 KR102214419 B1 KR 102214419B1 KR 1020157010847 A KR1020157010847 A KR 1020157010847A KR 20157010847 A KR20157010847 A KR 20157010847A KR 102214419 B1 KR102214419 B1 KR 102214419B1
- Authority
- KR
- South Korea
- Prior art keywords
- fluid
- well
- reaction tube
- chamber
- substrate
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 238000006243 chemical reaction Methods 0.000 title claims abstract description 138
- 239000012530 fluid Substances 0.000 claims abstract description 160
- 239000000758 substrate Substances 0.000 claims abstract description 137
- 238000003199 nucleic acid amplification method Methods 0.000 claims description 97
- 230000003321 amplification Effects 0.000 claims description 96
- 238000000034 method Methods 0.000 claims description 69
- 239000007788 liquid Substances 0.000 claims description 30
- 238000011049 filling Methods 0.000 claims description 20
- 239000000463 material Substances 0.000 claims description 15
- 238000010438 heat treatment Methods 0.000 claims description 12
- 230000002209 hydrophobic effect Effects 0.000 claims description 10
- 238000004891 communication Methods 0.000 claims description 9
- PXHVJJICTQNCMI-UHFFFAOYSA-N Nickel Chemical compound [Ni] PXHVJJICTQNCMI-UHFFFAOYSA-N 0.000 claims description 8
- 238000007789 sealing Methods 0.000 claims description 6
- 229910052759 nickel Inorganic materials 0.000 claims description 4
- 238000001816 cooling Methods 0.000 claims description 3
- 238000000151 deposition Methods 0.000 claims description 2
- 238000003825 pressing Methods 0.000 claims 2
- 239000000523 sample Substances 0.000 description 102
- 238000003752 polymerase chain reaction Methods 0.000 description 63
- 239000013615 primer Substances 0.000 description 61
- 150000007523 nucleic acids Chemical class 0.000 description 40
- 102000039446 nucleic acids Human genes 0.000 description 37
- 108020004707 nucleic acids Proteins 0.000 description 37
- 239000000872 buffer Substances 0.000 description 21
- 108091033319 polynucleotide Proteins 0.000 description 20
- 102000040430 polynucleotide Human genes 0.000 description 20
- 239000002157 polynucleotide Substances 0.000 description 20
- 108090000623 proteins and genes Proteins 0.000 description 20
- 238000001514 detection method Methods 0.000 description 19
- 239000003921 oil Substances 0.000 description 19
- 230000008569 process Effects 0.000 description 17
- 239000000047 product Substances 0.000 description 17
- 238000004458 analytical method Methods 0.000 description 16
- 125000003729 nucleotide group Chemical group 0.000 description 15
- 239000002773 nucleotide Substances 0.000 description 14
- 238000003753 real-time PCR Methods 0.000 description 13
- 230000027455 binding Effects 0.000 description 12
- 239000003153 chemical reaction reagent Substances 0.000 description 12
- -1 polypropylene Polymers 0.000 description 12
- 102000004169 proteins and genes Human genes 0.000 description 11
- 101100193693 Kirsten murine sarcoma virus K-RAS gene Proteins 0.000 description 10
- 239000003269 fluorescent indicator Substances 0.000 description 10
- 239000002480 mineral oil Substances 0.000 description 10
- 235000010446 mineral oil Nutrition 0.000 description 10
- 238000003556 assay Methods 0.000 description 9
- 239000012528 membrane Substances 0.000 description 9
- 229910052770 Uranium Inorganic materials 0.000 description 8
- 239000010408 film Substances 0.000 description 8
- 239000011541 reaction mixture Substances 0.000 description 8
- 238000012360 testing method Methods 0.000 description 8
- 108020004414 DNA Proteins 0.000 description 7
- 239000012491 analyte Substances 0.000 description 7
- 239000000126 substance Substances 0.000 description 7
- 108091093088 Amplicon Proteins 0.000 description 6
- 108091028043 Nucleic acid sequence Proteins 0.000 description 6
- 210000004027 cell Anatomy 0.000 description 6
- 239000007795 chemical reaction product Substances 0.000 description 6
- 230000000295 complement effect Effects 0.000 description 6
- 239000012634 fragment Substances 0.000 description 6
- 229910052751 metal Inorganic materials 0.000 description 6
- 239000002184 metal Substances 0.000 description 6
- 239000004743 Polypropylene Substances 0.000 description 5
- 238000013461 design Methods 0.000 description 5
- 238000002474 experimental method Methods 0.000 description 5
- 238000009396 hybridization Methods 0.000 description 5
- 238000007857 nested PCR Methods 0.000 description 5
- 229920001155 polypropylene Polymers 0.000 description 5
- 238000011160 research Methods 0.000 description 5
- 239000007787 solid Substances 0.000 description 5
- 239000000243 solution Substances 0.000 description 5
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 4
- 102000004190 Enzymes Human genes 0.000 description 4
- 108090000790 Enzymes Proteins 0.000 description 4
- 238000000137 annealing Methods 0.000 description 4
- 239000007864 aqueous solution Substances 0.000 description 4
- 239000000975 dye Substances 0.000 description 4
- 238000001704 evaporation Methods 0.000 description 4
- 230000008020 evaporation Effects 0.000 description 4
- 238000012545 processing Methods 0.000 description 4
- 238000009736 wetting Methods 0.000 description 4
- 108020004705 Codon Proteins 0.000 description 3
- 102000053602 DNA Human genes 0.000 description 3
- 239000003155 DNA primer Substances 0.000 description 3
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 3
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 3
- 108020005187 Oligonucleotide Probes Proteins 0.000 description 3
- 239000000427 antigen Substances 0.000 description 3
- 230000000712 assembly Effects 0.000 description 3
- 238000000429 assembly Methods 0.000 description 3
- 238000012864 cross contamination Methods 0.000 description 3
- 230000005284 excitation Effects 0.000 description 3
- 238000007306 functionalization reaction Methods 0.000 description 3
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 3
- 239000010931 gold Substances 0.000 description 3
- 238000007373 indentation Methods 0.000 description 3
- 238000007834 ligase chain reaction Methods 0.000 description 3
- 230000000670 limiting effect Effects 0.000 description 3
- 239000002751 oligonucleotide probe Substances 0.000 description 3
- 230000003287 optical effect Effects 0.000 description 3
- 238000007639 printing Methods 0.000 description 3
- 238000010839 reverse transcription Methods 0.000 description 3
- 238000003757 reverse transcription PCR Methods 0.000 description 3
- 230000002441 reversible effect Effects 0.000 description 3
- 239000010409 thin film Substances 0.000 description 3
- 210000001519 tissue Anatomy 0.000 description 3
- 238000012546 transfer Methods 0.000 description 3
- HPZMWTNATZPBIH-UHFFFAOYSA-N 1-methyladenine Chemical compound CN1C=NC2=NC=NC2=C1N HPZMWTNATZPBIH-UHFFFAOYSA-N 0.000 description 2
- RFLVMTUMFYRZCB-UHFFFAOYSA-N 1-methylguanine Chemical compound O=C1N(C)C(N)=NC2=C1N=CN2 RFLVMTUMFYRZCB-UHFFFAOYSA-N 0.000 description 2
- YSAJFXWTVFGPAX-UHFFFAOYSA-N 2-[(2,4-dioxo-1h-pyrimidin-5-yl)oxy]acetic acid Chemical compound OC(=O)COC1=CNC(=O)NC1=O YSAJFXWTVFGPAX-UHFFFAOYSA-N 0.000 description 2
- MJKVTPMWOKAVMS-UHFFFAOYSA-N 3-hydroxy-1-benzopyran-2-one Chemical compound C1=CC=C2OC(=O)C(O)=CC2=C1 MJKVTPMWOKAVMS-UHFFFAOYSA-N 0.000 description 2
- OVONXEQGWXGFJD-UHFFFAOYSA-N 4-sulfanylidene-1h-pyrimidin-2-one Chemical compound SC=1C=CNC(=O)N=1 OVONXEQGWXGFJD-UHFFFAOYSA-N 0.000 description 2
- OIVLITBTBDPEFK-UHFFFAOYSA-N 5,6-dihydrouracil Chemical compound O=C1CCNC(=O)N1 OIVLITBTBDPEFK-UHFFFAOYSA-N 0.000 description 2
- HSPHKCOAUOJLIO-UHFFFAOYSA-N 6-(aziridin-1-ylamino)-1h-pyrimidin-2-one Chemical compound N1C(=O)N=CC=C1NN1CC1 HSPHKCOAUOJLIO-UHFFFAOYSA-N 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 2
- 108090000626 DNA-directed RNA polymerases Proteins 0.000 description 2
- 102000004163 DNA-directed RNA polymerases Human genes 0.000 description 2
- 241000711549 Hepacivirus C Species 0.000 description 2
- 241000713772 Human immunodeficiency virus 1 Species 0.000 description 2
- 241000701806 Human papillomavirus Species 0.000 description 2
- HYVABZIGRDEKCD-UHFFFAOYSA-N N(6)-dimethylallyladenine Chemical compound CC(C)=CCNC1=NC=NC2=C1N=CN2 HYVABZIGRDEKCD-UHFFFAOYSA-N 0.000 description 2
- 108091034117 Oligonucleotide Proteins 0.000 description 2
- ZYFVNVRFVHJEIU-UHFFFAOYSA-N PicoGreen Chemical compound CN(C)CCCN(CCCN(C)C)C1=CC(=CC2=[N+](C3=CC=CC=C3S2)C)C2=CC=CC=C2N1C1=CC=CC=C1 ZYFVNVRFVHJEIU-UHFFFAOYSA-N 0.000 description 2
- 239000007983 Tris buffer Substances 0.000 description 2
- 241000700605 Viruses Species 0.000 description 2
- 239000000853 adhesive Substances 0.000 description 2
- 230000001070 adhesive effect Effects 0.000 description 2
- WYTGDNHDOZPMIW-RCBQFDQVSA-N alstonine Natural products C1=CC2=C3C=CC=CC3=NC2=C2N1C[C@H]1[C@H](C)OC=C(C(=O)OC)[C@H]1C2 WYTGDNHDOZPMIW-RCBQFDQVSA-N 0.000 description 2
- 108091007433 antigens Proteins 0.000 description 2
- 102000036639 antigens Human genes 0.000 description 2
- 238000013459 approach Methods 0.000 description 2
- 238000002820 assay format Methods 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 238000004925 denaturation Methods 0.000 description 2
- 230000036425 denaturation Effects 0.000 description 2
- 239000004205 dimethyl polysiloxane Substances 0.000 description 2
- 239000012153 distilled water Substances 0.000 description 2
- 238000005553 drilling Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000004049 embossing Methods 0.000 description 2
- 238000002866 fluorescence resonance energy transfer Methods 0.000 description 2
- 239000000989 food dye Substances 0.000 description 2
- 238000004108 freeze drying Methods 0.000 description 2
- 229910052737 gold Inorganic materials 0.000 description 2
- 230000036571 hydration Effects 0.000 description 2
- 238000006703 hydration reaction Methods 0.000 description 2
- 238000002347 injection Methods 0.000 description 2
- 239000007924 injection Substances 0.000 description 2
- 238000001746 injection moulding Methods 0.000 description 2
- 238000009830 intercalation Methods 0.000 description 2
- 238000011068 loading method Methods 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 238000002844 melting Methods 0.000 description 2
- 230000008018 melting Effects 0.000 description 2
- 108020004999 messenger RNA Proteins 0.000 description 2
- 108091070501 miRNA Proteins 0.000 description 2
- 239000002679 microRNA Substances 0.000 description 2
- 238000002493 microarray Methods 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012544 monitoring process Methods 0.000 description 2
- 238000000465 moulding Methods 0.000 description 2
- 239000002777 nucleoside Substances 0.000 description 2
- 230000036961 partial effect Effects 0.000 description 2
- BASFCYQUMIYNBI-UHFFFAOYSA-N platinum Chemical compound [Pt] BASFCYQUMIYNBI-UHFFFAOYSA-N 0.000 description 2
- 229920000435 poly(dimethylsiloxane) Polymers 0.000 description 2
- 229920006254 polymer film Polymers 0.000 description 2
- 239000002987 primer (paints) Substances 0.000 description 2
- 102000004196 processed proteins & peptides Human genes 0.000 description 2
- 108090000765 processed proteins & peptides Proteins 0.000 description 2
- 238000000159 protein binding assay Methods 0.000 description 2
- 238000011002 quantification Methods 0.000 description 2
- 230000004044 response Effects 0.000 description 2
- 230000000717 retained effect Effects 0.000 description 2
- 238000005096 rolling process Methods 0.000 description 2
- 230000009870 specific binding Effects 0.000 description 2
- ACOJCCLIDPZYJC-UHFFFAOYSA-M thiazole orange Chemical compound CC1=CC=C(S([O-])(=O)=O)C=C1.C1=CC=C2C(C=C3N(C4=CC=CC=C4S3)C)=CC=[N+](C)C2=C1 ACOJCCLIDPZYJC-UHFFFAOYSA-M 0.000 description 2
- RWQNBRDOKXIBIV-UHFFFAOYSA-N thymine Chemical compound CC1=CNC(=O)NC1=O RWQNBRDOKXIBIV-UHFFFAOYSA-N 0.000 description 2
- 238000011282 treatment Methods 0.000 description 2
- 235000011178 triphosphate Nutrition 0.000 description 2
- 239000001226 triphosphate Substances 0.000 description 2
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 2
- 108700026220 vif Genes Proteins 0.000 description 2
- 230000003612 virological effect Effects 0.000 description 2
- 239000011534 wash buffer Substances 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- SATCOUWSAZBIJO-UHFFFAOYSA-N 1-methyladenine Natural products N=C1N(C)C=NC2=C1NC=N2 SATCOUWSAZBIJO-UHFFFAOYSA-N 0.000 description 1
- WJNGQIYEQLPJMN-IOSLPCCCSA-N 1-methylinosine Chemical compound C1=NC=2C(=O)N(C)C=NC=2N1[C@@H]1O[C@H](CO)[C@@H](O)[C@H]1O WJNGQIYEQLPJMN-IOSLPCCCSA-N 0.000 description 1
- HLYBTPMYFWWNJN-UHFFFAOYSA-N 2-(2,4-dioxo-1h-pyrimidin-5-yl)-2-hydroxyacetic acid Chemical compound OC(=O)C(O)C1=CNC(=O)NC1=O HLYBTPMYFWWNJN-UHFFFAOYSA-N 0.000 description 1
- SGAKLDIYNFXTCK-UHFFFAOYSA-N 2-[(2,4-dioxo-1h-pyrimidin-5-yl)methylamino]acetic acid Chemical compound OC(=O)CNCC1=CNC(=O)NC1=O SGAKLDIYNFXTCK-UHFFFAOYSA-N 0.000 description 1
- SVBOROZXXYRWJL-UHFFFAOYSA-N 2-[(4-oxo-2-sulfanylidene-1h-pyrimidin-5-yl)methylamino]acetic acid Chemical compound OC(=O)CNCC1=CNC(=S)NC1=O SVBOROZXXYRWJL-UHFFFAOYSA-N 0.000 description 1
- XQCZBXHVTFVIFE-UHFFFAOYSA-N 2-amino-4-hydroxypyrimidine Chemical compound NC1=NC=CC(O)=N1 XQCZBXHVTFVIFE-UHFFFAOYSA-N 0.000 description 1
- XMSMHKMPBNTBOD-UHFFFAOYSA-N 2-dimethylamino-6-hydroxypurine Chemical compound N1C(N(C)C)=NC(=O)C2=C1N=CN2 XMSMHKMPBNTBOD-UHFFFAOYSA-N 0.000 description 1
- SMADWRYCYBUIKH-UHFFFAOYSA-N 2-methyl-7h-purin-6-amine Chemical compound CC1=NC(N)=C2NC=NC2=N1 SMADWRYCYBUIKH-UHFFFAOYSA-N 0.000 description 1
- WEVYNIUIFUYDGI-UHFFFAOYSA-N 3-[6-[4-(trifluoromethoxy)anilino]-4-pyrimidinyl]benzamide Chemical compound NC(=O)C1=CC=CC(C=2N=CN=C(NC=3C=CC(OC(F)(F)F)=CC=3)C=2)=C1 WEVYNIUIFUYDGI-UHFFFAOYSA-N 0.000 description 1
- QWZHDKGQKYEBKK-UHFFFAOYSA-N 3-aminochromen-2-one Chemical compound C1=CC=C2OC(=O)C(N)=CC2=C1 QWZHDKGQKYEBKK-UHFFFAOYSA-N 0.000 description 1
- KOLPWZCZXAMXKS-UHFFFAOYSA-N 3-methylcytosine Chemical compound CN1C(N)=CC=NC1=O KOLPWZCZXAMXKS-UHFFFAOYSA-N 0.000 description 1
- GJAKJCICANKRFD-UHFFFAOYSA-N 4-acetyl-4-amino-1,3-dihydropyrimidin-2-one Chemical compound CC(=O)C1(N)NC(=O)NC=C1 GJAKJCICANKRFD-UHFFFAOYSA-N 0.000 description 1
- MQJSSLBGAQJNER-UHFFFAOYSA-N 5-(methylaminomethyl)-1h-pyrimidine-2,4-dione Chemical compound CNCC1=CNC(=O)NC1=O MQJSSLBGAQJNER-UHFFFAOYSA-N 0.000 description 1
- KELXHQACBIUYSE-UHFFFAOYSA-N 5-methoxy-1h-pyrimidine-2,4-dione Chemical compound COC1=CNC(=O)NC1=O KELXHQACBIUYSE-UHFFFAOYSA-N 0.000 description 1
- ZLAQATDNGLKIEV-UHFFFAOYSA-N 5-methyl-2-sulfanylidene-1h-pyrimidin-4-one Chemical compound CC1=CNC(=S)NC1=O ZLAQATDNGLKIEV-UHFFFAOYSA-N 0.000 description 1
- LRSASMSXMSNRBT-UHFFFAOYSA-N 5-methylcytosine Chemical compound CC1=CNC(=O)N=C1N LRSASMSXMSNRBT-UHFFFAOYSA-N 0.000 description 1
- DCPSTSVLRXOYGS-UHFFFAOYSA-N 6-amino-1h-pyrimidine-2-thione Chemical compound NC1=CC=NC(S)=N1 DCPSTSVLRXOYGS-UHFFFAOYSA-N 0.000 description 1
- CKOMXBHMKXXTNW-UHFFFAOYSA-N 6-methyladenine Chemical compound CNC1=NC=NC2=C1N=CN2 CKOMXBHMKXXTNW-UHFFFAOYSA-N 0.000 description 1
- SWJYOKZMYFJUOY-KQYNXXCUSA-N 9-[(2r,3r,4s,5r)-3,4-dihydroxy-5-(hydroxymethyl)oxolan-2-yl]-6-(methylamino)-7h-purin-8-one Chemical compound OC1=NC=2C(NC)=NC=NC=2N1[C@@H]1O[C@H](CO)[C@@H](O)[C@H]1O SWJYOKZMYFJUOY-KQYNXXCUSA-N 0.000 description 1
- 102000007469 Actins Human genes 0.000 description 1
- 108010085238 Actins Proteins 0.000 description 1
- 108700028369 Alleles Proteins 0.000 description 1
- 101100519158 Arabidopsis thaliana PCR2 gene Proteins 0.000 description 1
- 229910001020 Au alloy Inorganic materials 0.000 description 1
- 102000036365 BRCA1 Human genes 0.000 description 1
- 108700020463 BRCA1 Proteins 0.000 description 1
- 101150072950 BRCA1 gene Proteins 0.000 description 1
- 102000052609 BRCA2 Human genes 0.000 description 1
- 108700020462 BRCA2 Proteins 0.000 description 1
- 241000193738 Bacillus anthracis Species 0.000 description 1
- 101150008921 Brca2 gene Proteins 0.000 description 1
- 102100025064 Cellular tumor antigen p53 Human genes 0.000 description 1
- 241000606153 Chlamydia trachomatis Species 0.000 description 1
- 241000701022 Cytomegalovirus Species 0.000 description 1
- 101100239628 Danio rerio myca gene Proteins 0.000 description 1
- 101150029707 ERBB2 gene Proteins 0.000 description 1
- 241000194032 Enterococcus faecalis Species 0.000 description 1
- 241000709661 Enterovirus Species 0.000 description 1
- 239000004593 Epoxy Substances 0.000 description 1
- 241000710831 Flavivirus Species 0.000 description 1
- GHASVSINZRGABV-UHFFFAOYSA-N Fluorouracil Chemical compound FC1=CNC(=O)NC1=O GHASVSINZRGABV-UHFFFAOYSA-N 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 102100031181 Glyceraldehyde-3-phosphate dehydrogenase Human genes 0.000 description 1
- IWYRWIUNAVNFPE-UHFFFAOYSA-N Glycidaldehyde Chemical compound O=CC1CO1 IWYRWIUNAVNFPE-UHFFFAOYSA-N 0.000 description 1
- 241000590002 Helicobacter pylori Species 0.000 description 1
- 241000282412 Homo Species 0.000 description 1
- 101000721661 Homo sapiens Cellular tumor antigen p53 Proteins 0.000 description 1
- 241000701085 Human alphaherpesvirus 3 Species 0.000 description 1
- 241000701024 Human betaherpesvirus 5 Species 0.000 description 1
- UGQMRVRMYYASKQ-KQYNXXCUSA-N Inosine Chemical compound O[C@@H]1[C@H](O)[C@@H](CO)O[C@H]1N1C2=NC=NC(O)=C2N=C1 UGQMRVRMYYASKQ-KQYNXXCUSA-N 0.000 description 1
- 229930010555 Inosine Natural products 0.000 description 1
- 241000589248 Legionella Species 0.000 description 1
- 208000007764 Legionnaires' Disease Diseases 0.000 description 1
- 102000003960 Ligases Human genes 0.000 description 1
- 108090000364 Ligases Proteins 0.000 description 1
- 241000186779 Listeria monocytogenes Species 0.000 description 1
- 101150039798 MYC gene Proteins 0.000 description 1
- 241000701076 Macacine alphaherpesvirus 1 Species 0.000 description 1
- 201000009906 Meningitis Diseases 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 108700011259 MicroRNAs Proteins 0.000 description 1
- 241000187479 Mycobacterium tuberculosis Species 0.000 description 1
- SGSSKEDGVONRGC-UHFFFAOYSA-N N(2)-methylguanine Chemical compound O=C1NC(NC)=NC2=C1N=CN2 SGSSKEDGVONRGC-UHFFFAOYSA-N 0.000 description 1
- KMIQIMURDQPREV-UHFFFAOYSA-N NC1(NC(C2=NC=NC2=N1)(NCCC(=C)C)N)SC Chemical compound NC1(NC(C2=NC=NC2=N1)(NCCC(=C)C)N)SC KMIQIMURDQPREV-UHFFFAOYSA-N 0.000 description 1
- 241000588653 Neisseria Species 0.000 description 1
- 241000588652 Neisseria gonorrhoeae Species 0.000 description 1
- 229910000990 Ni alloy Inorganic materials 0.000 description 1
- 241001263478 Norovirus Species 0.000 description 1
- 102000043276 Oncogene Human genes 0.000 description 1
- 108700020796 Oncogene Proteins 0.000 description 1
- 102000014160 PTEN Phosphohydrolase Human genes 0.000 description 1
- 108010011536 PTEN Phosphohydrolase Proteins 0.000 description 1
- 241000710778 Pestivirus Species 0.000 description 1
- 108010004729 Phycoerythrin Proteins 0.000 description 1
- 241000709664 Picornaviridae Species 0.000 description 1
- 241000125945 Protoparvovirus Species 0.000 description 1
- 229910001260 Pt alloy Inorganic materials 0.000 description 1
- CGNLCCVKSWNSDG-UHFFFAOYSA-N SYBR Green I Chemical compound CN(C)CCCN(CCC)C1=CC(C=C2N(C3=CC=CC=C3S2)C)=C2C=CC=CC2=[N+]1C1=CC=CC=C1 CGNLCCVKSWNSDG-UHFFFAOYSA-N 0.000 description 1
- 241000239226 Scorpiones Species 0.000 description 1
- 108020004682 Single-Stranded DNA Proteins 0.000 description 1
- 108010006785 Taq Polymerase Proteins 0.000 description 1
- 101710183280 Topoisomerase Proteins 0.000 description 1
- 102000044209 Tumor Suppressor Genes Human genes 0.000 description 1
- 108700025716 Tumor Suppressor Genes Proteins 0.000 description 1
- 108010019530 Vascular Endothelial Growth Factors Proteins 0.000 description 1
- 102000005789 Vascular Endothelial Growth Factors Human genes 0.000 description 1
- 108010067390 Viral Proteins Proteins 0.000 description 1
- 101100459258 Xenopus laevis myc-a gene Proteins 0.000 description 1
- ULHRKLSNHXXJLO-UHFFFAOYSA-L Yo-Pro-1 Chemical compound [I-].[I-].C1=CC=C2C(C=C3N(C4=CC=CC=C4O3)C)=CC=[N+](CCC[N+](C)(C)C)C2=C1 ULHRKLSNHXXJLO-UHFFFAOYSA-L 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- NIXOWILDQLNWCW-UHFFFAOYSA-N acrylic acid group Chemical group C(C=C)(=O)O NIXOWILDQLNWCW-UHFFFAOYSA-N 0.000 description 1
- 108010004469 allophycocyanin Proteins 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 238000004166 bioassay Methods 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
- 210000001124 body fluid Anatomy 0.000 description 1
- 239000010839 body fluid Substances 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 235000014633 carbohydrates Nutrition 0.000 description 1
- CZPLANDPABRVHX-UHFFFAOYSA-N cascade blue Chemical compound C=1C2=CC=CC=C2C(NCC)=CC=1C(C=1C=CC(=CC=1)N(CC)CC)=C1C=CC(=[N+](CC)CC)C=C1 CZPLANDPABRVHX-UHFFFAOYSA-N 0.000 description 1
- 239000000919 ceramic Substances 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 230000000739 chaotic effect Effects 0.000 description 1
- 239000012295 chemical reaction liquid Substances 0.000 description 1
- 229940038705 chlamydia trachomatis Drugs 0.000 description 1
- 230000002860 competitive effect Effects 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 230000008878 coupling Effects 0.000 description 1
- 238000010168 coupling process Methods 0.000 description 1
- 238000005859 coupling reaction Methods 0.000 description 1
- 238000013211 curve analysis Methods 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 230000008021 deposition Effects 0.000 description 1
- 229910003460 diamond Inorganic materials 0.000 description 1
- 239000010432 diamond Substances 0.000 description 1
- 238000007599 discharging Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000006073 displacement reaction Methods 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 238000005323 electroforming Methods 0.000 description 1
- 239000012149 elution buffer Substances 0.000 description 1
- 229940032049 enterococcus faecalis Drugs 0.000 description 1
- 102000052116 epidermal growth factor receptor activity proteins Human genes 0.000 description 1
- 108700015053 epidermal growth factor receptor activity proteins Proteins 0.000 description 1
- 230000001973 epigenetic effect Effects 0.000 description 1
- ZMMJGEGLRURXTF-UHFFFAOYSA-N ethidium bromide Chemical compound [Br-].C12=CC(N)=CC=C2C2=CC=C(N)C=C2[N+](CC)=C1C1=CC=CC=C1 ZMMJGEGLRURXTF-UHFFFAOYSA-N 0.000 description 1
- 229960005542 ethidium bromide Drugs 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 238000005429 filling process Methods 0.000 description 1
- GNBHRKFJIUUOQI-UHFFFAOYSA-N fluorescein Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 GNBHRKFJIUUOQI-UHFFFAOYSA-N 0.000 description 1
- 239000007850 fluorescent dye Substances 0.000 description 1
- 229960002949 fluorouracil Drugs 0.000 description 1
- 239000011888 foil Substances 0.000 description 1
- 125000000524 functional group Chemical group 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 108020004445 glyceraldehyde-3-phosphate dehydrogenase Proteins 0.000 description 1
- 239000001046 green dye Substances 0.000 description 1
- 229940037467 helicobacter pylori Drugs 0.000 description 1
- 208000002672 hepatitis B Diseases 0.000 description 1
- 230000033444 hydroxylation Effects 0.000 description 1
- 238000005805 hydroxylation reaction Methods 0.000 description 1
- 238000010324 immunological assay Methods 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 206010022000 influenza Diseases 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 230000000977 initiatory effect Effects 0.000 description 1
- 238000007641 inkjet printing Methods 0.000 description 1
- 229960003786 inosine Drugs 0.000 description 1
- 230000002687 intercalation Effects 0.000 description 1
- 229910052747 lanthanoid Inorganic materials 0.000 description 1
- 150000002602 lanthanoids Chemical class 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 238000001459 lithography Methods 0.000 description 1
- DLBFLQKQABVKGT-UHFFFAOYSA-L lucifer yellow dye Chemical compound [Li+].[Li+].[O-]S(=O)(=O)C1=CC(C(N(C(=O)NN)C2=O)=O)=C3C2=CC(S([O-])(=O)=O)=CC3=C1N DLBFLQKQABVKGT-UHFFFAOYSA-L 0.000 description 1
- 238000007403 mPCR Methods 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- 230000001404 mediated effect Effects 0.000 description 1
- 239000007769 metal material Substances 0.000 description 1
- IZAGSTRIDUNNOY-UHFFFAOYSA-N methyl 2-[(2,4-dioxo-1h-pyrimidin-5-yl)oxy]acetate Chemical compound COC(=O)COC1=CNC(=O)NC1=O IZAGSTRIDUNNOY-UHFFFAOYSA-N 0.000 description 1
- 150000004702 methyl esters Chemical class 0.000 description 1
- 230000011987 methylation Effects 0.000 description 1
- 238000007069 methylation reaction Methods 0.000 description 1
- 238000010369 molecular cloning Methods 0.000 description 1
- 230000035772 mutation Effects 0.000 description 1
- YOHYSYJDKVYCJI-UHFFFAOYSA-N n-[3-[[6-[3-(trifluoromethyl)anilino]pyrimidin-4-yl]amino]phenyl]cyclopropanecarboxamide Chemical compound FC(F)(F)C1=CC=CC(NC=2N=CN=C(NC=3C=C(NC(=O)C4CC4)C=CC=3)C=2)=C1 YOHYSYJDKVYCJI-UHFFFAOYSA-N 0.000 description 1
- 239000013642 negative control Substances 0.000 description 1
- 239000000346 nonvolatile oil Substances 0.000 description 1
- 238000001668 nucleic acid synthesis Methods 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 230000003071 parasitic effect Effects 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 239000002861 polymer material Substances 0.000 description 1
- 229920001184 polypeptide Polymers 0.000 description 1
- 239000012462 polypropylene substrate Substances 0.000 description 1
- 229920000136 polysorbate Polymers 0.000 description 1
- 239000013641 positive control Substances 0.000 description 1
- 239000002243 precursor Substances 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- QQONPFPTGQHPMA-UHFFFAOYSA-N propylene Natural products CC=C QQONPFPTGQHPMA-UHFFFAOYSA-N 0.000 description 1
- 125000004805 propylene group Chemical group [H]C([H])([H])C([H])([*:1])C([H])([H])[*:2] 0.000 description 1
- 230000002829 reductive effect Effects 0.000 description 1
- 230000010076 replication Effects 0.000 description 1
- 238000012552 review Methods 0.000 description 1
- PYWVYCXTNDRMGF-UHFFFAOYSA-N rhodamine B Chemical compound [Cl-].C=12C=CC(=[N+](CC)CC)C=C2OC2=CC(N(CC)CC)=CC=C2C=1C1=CC=CC=C1C(O)=O PYWVYCXTNDRMGF-UHFFFAOYSA-N 0.000 description 1
- 108020004418 ribosomal RNA Proteins 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 238000004381 surface treatment Methods 0.000 description 1
- 239000004094 surface-active agent Substances 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- MPLHNVLQVRSVEE-UHFFFAOYSA-N texas red Chemical compound [O-]S(=O)(=O)C1=CC(S(Cl)(=O)=O)=CC=C1C(C1=CC=2CCCN3CCCC(C=23)=C1O1)=C2C1=C(CCC1)C3=[N+]1CCCC3=C2 MPLHNVLQVRSVEE-UHFFFAOYSA-N 0.000 description 1
- 238000013518 transcription Methods 0.000 description 1
- 230000035897 transcription Effects 0.000 description 1
- 239000012780 transparent material Substances 0.000 description 1
- BPSIOYPQMFLKFR-UHFFFAOYSA-N trimethoxy-[3-(oxiran-2-ylmethoxy)propyl]silane Chemical compound CO[Si](OC)(OC)CCCOCC1CO1 BPSIOYPQMFLKFR-UHFFFAOYSA-N 0.000 description 1
- 201000008827 tuberculosis Diseases 0.000 description 1
- 241001529453 unidentified herpesvirus Species 0.000 description 1
- 229940035893 uracil Drugs 0.000 description 1
- 238000012800 visualization Methods 0.000 description 1
Images
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M1/00—Apparatus for enzymology or microbiology
- C12M1/36—Apparatus for enzymology or microbiology including condition or time responsive control, e.g. automatically controlled fermentors
- C12M1/38—Temperature-responsive control
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/483—Physical analysis of biological material
- G01N33/487—Physical analysis of biological material of liquid biological material
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/08—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a stream of discrete samples flowing along a tube system, e.g. flow injection analysis
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/06—Fluid handling related problems
- B01L2200/0642—Filling fluids into wells by specific techniques
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/06—Fluid handling related problems
- B01L2200/0673—Handling of plugs of fluid surrounded by immiscible fluid
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/06—Auxiliary integrated devices, integrated components
- B01L2300/0627—Sensor or part of a sensor is integrated
- B01L2300/0636—Integrated biosensor, microarrays
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0809—Geometry, shape and general structure rectangular shaped
- B01L2300/0819—Microarrays; Biochips
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
- B01L2400/0406—Moving fluids with specific forces or mechanical means specific forces capillary forces
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
- B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
- B01L3/5085—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates
- B01L3/50851—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates specially adapted for heating or cooling samples
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L7/00—Heating or cooling apparatus; Heat insulating devices
- B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Hematology (AREA)
- Clinical Laboratory Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biochemistry (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- General Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Dispersion Chemistry (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Biomedical Technology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Urology & Nephrology (AREA)
- Medicinal Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
- Food Science & Technology (AREA)
- Physical Or Chemical Processes And Apparatus (AREA)
- Sustainable Development (AREA)
- Cell Biology (AREA)
- External Artificial Organs (AREA)
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201261706115P | 2012-09-26 | 2012-09-26 | |
| US61/706,115 | 2012-09-26 | ||
| US13/843,739 | 2013-03-15 | ||
| US13/843,739 US9914968B2 (en) | 2012-09-26 | 2013-03-15 | Honeycomb tube |
| PCT/US2013/062042 WO2014052671A1 (en) | 2012-09-26 | 2013-09-26 | Honeycomb tube |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| KR20150067222A KR20150067222A (ko) | 2015-06-17 |
| KR102214419B1 true KR102214419B1 (ko) | 2021-02-08 |
Family
ID=50339439
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| KR1020157010847A Active KR102214419B1 (ko) | 2012-09-26 | 2013-09-26 | 벌집형 반응튜브 |
Country Status (12)
| Country | Link |
|---|---|
| US (10) | US9914968B2 (enExample) |
| JP (2) | JP6525877B2 (enExample) |
| KR (1) | KR102214419B1 (enExample) |
| CN (2) | CN105051546A (enExample) |
| AP (1) | AP2015008394A0 (enExample) |
| AU (1) | AU2013323428B2 (enExample) |
| BR (1) | BR112015006566B1 (enExample) |
| CA (1) | CA2886484C (enExample) |
| EA (1) | EA031989B1 (enExample) |
| MX (1) | MX363399B (enExample) |
| WO (1) | WO2014052671A1 (enExample) |
| ZA (1) | ZA201502463B (enExample) |
Families Citing this family (39)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108587867B (zh) | 2012-05-25 | 2021-12-17 | 北卡罗来纳-查佩尔山大学 | 微流体装置、用于试剂的固体支持体和相关方法 |
| US9914968B2 (en) | 2012-09-26 | 2018-03-13 | Cepheid | Honeycomb tube |
| SG2012075792A (en) * | 2012-10-09 | 2014-05-29 | Jn Medsys Pte Ltd | An improved device and method |
| BR112015023079B1 (pt) * | 2013-03-15 | 2020-12-29 | Cepheid | aparelho |
| US11028432B2 (en) * | 2013-11-05 | 2021-06-08 | Biofire Diagnostics, Llc | Induction PCR |
| EP3207472B1 (en) | 2014-10-16 | 2024-11-27 | Cepheid | Biosecurity screening system and method |
| RU2712610C2 (ru) * | 2015-04-03 | 2020-01-29 | Эбботт Лэборетриз | Устройства и способы для анализа образца |
| CN108025304B (zh) * | 2015-07-22 | 2022-01-21 | 北卡罗来纳-查佩尔山大学 | 含有具有空间分离的珠保留和信号检测段的珠孔几何结构的流体装置和相关方法 |
| US10797567B2 (en) * | 2015-07-23 | 2020-10-06 | Life Technologies Corporation | Rotor assembly including a housing for a sensor array component and methods for using same |
| HK1252768A1 (zh) | 2015-07-24 | 2019-05-31 | 塞弗德公司 | 分子诊断化验系统 |
| JP6808940B2 (ja) * | 2016-01-29 | 2021-01-06 | 凸版印刷株式会社 | アレイデバイス、生体分子解析用キット及び溶媒の封止方法 |
| GB2554377A (en) * | 2016-09-23 | 2018-04-04 | Dnanudge Ltd | Method and apparatus for analysing a biological sample |
| KR101997116B1 (ko) * | 2016-10-14 | 2019-07-05 | 연세대학교 산학협력단 | Kras 유전자에 상보적인 가이드 rna 및 이의 용도 |
| WO2018136861A1 (en) * | 2017-01-23 | 2018-07-26 | America Diagnosis, Inc. | Direct quantitative pcr device and method of use thereof |
| CN110769925A (zh) * | 2017-03-29 | 2020-02-07 | 康奈尔大学 | 使用组合的核酸酶、连结酶、聚合酶以及测序反应测定核酸序列、表达、拷贝数或甲基化变化的装置、方法以及系统 |
| WO2018185871A1 (ja) * | 2017-04-05 | 2018-10-11 | 株式会社日立ハイテクノロジーズ | 核酸増幅方法および核酸解析用装置 |
| WO2019131592A1 (ja) * | 2017-12-26 | 2019-07-04 | 凸版印刷株式会社 | 標的分子の検出における偽陰性判定の発生を抑制する方法および検出デバイス |
| PL425106A1 (pl) | 2018-03-30 | 2019-10-07 | Bacteromic Spółka Z Ograniczoną Odpowiedzialnością | Chip mikroprzepływowy |
| CN111218383B (zh) * | 2018-11-26 | 2025-09-09 | 杭州比格飞序生物科技有限公司 | 一种核酸提取装置及其方法 |
| EP3897981B1 (en) * | 2018-12-20 | 2023-06-07 | Imec VZW | Addressable micro-reaction chamber array |
| EP3941630B1 (en) * | 2019-03-18 | 2025-04-16 | Cellular Research, Inc. | Precise delivery of components into fluids |
| US20220170071A1 (en) | 2019-03-21 | 2022-06-02 | Sherlock Biosciences, Inc. | System |
| JP7280101B2 (ja) | 2019-04-23 | 2023-05-23 | 株式会社日立製作所 | 測定セル製造方法、測定セル |
| CN119955908A (zh) * | 2019-05-08 | 2025-05-09 | Dnae诊断有限公司 | 组合的液相和固相dna扩增 |
| CN113892035B (zh) * | 2019-05-30 | 2025-05-09 | 凸版印刷株式会社 | 向孔中导入液体的方法 |
| US10820847B1 (en) | 2019-08-15 | 2020-11-03 | Talis Biomedical Corporation | Diagnostic system |
| US12054320B2 (en) | 2019-09-23 | 2024-08-06 | Cepheid | Multi-chambered lid apparatus with reagent port |
| BR112022005373A2 (pt) * | 2019-09-23 | 2022-06-14 | Cepheid | Aparelho de tampa de múltiplas câmaras com porta de reagente |
| US11254979B2 (en) | 2020-06-01 | 2022-02-22 | Shaheen Innovations Holding Limited | Systems and devices for infectious disease screening |
| US11730193B2 (en) | 2019-12-15 | 2023-08-22 | Shaheen Innovations Holding Limited | Hookah device |
| CN111190021B (zh) * | 2020-02-22 | 2023-08-11 | 武汉泰沃科技有限责任公司 | 一种生物样品处理盒 |
| WO2021245390A1 (en) | 2020-06-01 | 2021-12-09 | Shaheen Innovations Holding Limited | An infectious disease screening system |
| GB2635034B (en) | 2020-06-01 | 2025-09-03 | Shaheen Innovations Holding Ltd | An infectious disease screening device |
| KR20230058722A (ko) * | 2020-09-29 | 2023-05-03 | 주식회사 씨젠 | 핵산 반응 챔버, 이를 이용한 핵산 반응 방법 및 이를 포함하는 샘플 처리용 카트리지 |
| AU2022207990A1 (en) | 2021-01-13 | 2023-08-17 | Cepheid | Lossy mechatronic systems and methods of estimation |
| CN115836222A (zh) * | 2021-05-27 | 2023-03-21 | 京东方科技集团股份有限公司 | 检测芯片及其制备方法和进样方法 |
| CN118234564A (zh) * | 2021-07-09 | 2024-06-21 | 塞弗德公司 | 高级多路复用反应容器、试剂点样装置和相关方法 |
| JP2024531451A (ja) * | 2021-08-25 | 2024-08-29 | インテグリス・インコーポレーテッド | 剛性フレームバッグ |
| WO2024263523A1 (en) | 2023-06-21 | 2024-12-26 | Cepheid | Real-time monitoring of liquid temperature |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20060263242A1 (en) | 2005-04-28 | 2006-11-23 | Fuji Photo Film Co., Ltd. | Microchip and analysis method using the same |
| JP2011506998A (ja) | 2007-12-17 | 2011-03-03 | ハイ−チング ゴング | マイクロ流体デバイス |
Family Cites Families (55)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4683195A (en) | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
| US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
| US4965188A (en) | 1986-08-22 | 1990-10-23 | Cetus Corporation | Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme |
| US4800159A (en) | 1986-02-07 | 1989-01-24 | Cetus Corporation | Process for amplifying, detecting, and/or cloning nucleic acid sequences |
| US4988617A (en) | 1988-03-25 | 1991-01-29 | California Institute Of Technology | Method of detecting a nucleotide change in nucleic acids |
| US5168038A (en) | 1988-06-17 | 1992-12-01 | The Board Of Trustees Of The Leland Stanford Junior University | In situ transcription in cells and tissues |
| CA2020958C (en) | 1989-07-11 | 2005-01-11 | Daniel L. Kacian | Nucleic acid sequence amplification methods |
| JPH03126208A (ja) | 1989-10-11 | 1991-05-29 | Nippon Chemicon Corp | 電解コンデンサ用電解液 |
| US5427930A (en) | 1990-01-26 | 1995-06-27 | Abbott Laboratories | Amplification of target nucleic acids using gap filling ligase chain reaction |
| US5210015A (en) | 1990-08-06 | 1993-05-11 | Hoffman-La Roche Inc. | Homogeneous assay system using the nuclease activity of a nucleic acid polymerase |
| SE9002579D0 (sv) * | 1990-08-07 | 1990-08-07 | Pharmacia Ab | Method and apparatus for carrying out biochemical reactions |
| US5455166A (en) | 1991-01-31 | 1995-10-03 | Becton, Dickinson And Company | Strand displacement amplification |
| JP3080178B2 (ja) | 1991-02-18 | 2000-08-21 | 東洋紡績株式会社 | 核酸配列の増幅方法およびそのための試薬キット |
| US5925517A (en) | 1993-11-12 | 1999-07-20 | The Public Health Research Institute Of The City Of New York, Inc. | Detectably labeled dual conformation oligonucleotide probes, assays and kits |
| US5648211A (en) | 1994-04-18 | 1997-07-15 | Becton, Dickinson And Company | Strand displacement amplification using thermophilic enzymes |
| US5854033A (en) | 1995-11-21 | 1998-12-29 | Yale University | Rolling circle replication reporter systems |
| DK1179600T3 (da) | 1996-06-04 | 2005-09-05 | Univ Utah Res Found | Overvågning af hybridisering under PCR |
| GB9620934D0 (en) * | 1996-10-08 | 1996-11-27 | Molecular Drives Limited | Multi-well containers |
| US6818185B1 (en) * | 1999-05-28 | 2004-11-16 | Cepheid | Cartridge for conducting a chemical reaction |
| US6403037B1 (en) * | 2000-02-04 | 2002-06-11 | Cepheid | Reaction vessel and temperature control system |
| CA2311622A1 (en) * | 2000-06-15 | 2001-12-15 | Moussa Hoummady | Sub-nanoliter liquid drop dispensing system and method therefor |
| US8048386B2 (en) | 2002-02-25 | 2011-11-01 | Cepheid | Fluid processing and control |
| CA2425476C (en) | 2000-10-10 | 2011-02-01 | Biotrove, Inc. | Apparatus for assay, synthesis and storage, and methods of manufacture, use, and manipulation thereof |
| US20080202927A1 (en) * | 2000-11-03 | 2008-08-28 | Clinical Micro Sensors, Inc. | Devices and methods for biochip multiplexing |
| US8277753B2 (en) | 2002-08-23 | 2012-10-02 | Life Technologies Corporation | Microfluidic transfer pin |
| GB0321158D0 (en) * | 2003-09-10 | 2003-10-08 | Central Research Lab Ltd | Apparatus and method for handling cells,embryos or oocytes |
| CN1942590B (zh) | 2004-02-18 | 2012-09-05 | 周小川 | 多元化学和生化反应的流体装置和方法 |
| US7414118B1 (en) | 2004-04-14 | 2008-08-19 | Applied Biosystems Inc. | Modified oligonucleotides and applications thereof |
| MXPA06014185A (es) | 2004-06-07 | 2007-04-16 | Strides Arcolab Ltd | Composicion farmaceutica que contiene una solucion estable y clara de medicamento anti-inflmatorio en capsulas de gelatina blanda, y proceso para producirlo. |
| US7473533B2 (en) | 2004-12-30 | 2009-01-06 | Corning Incorporated | Membrane arrays and methods of manufacture |
| GB2423710A (en) | 2005-03-01 | 2006-09-06 | Boots Healthcare Int Ltd | Lozenge production process |
| WO2007002588A2 (en) * | 2005-06-23 | 2007-01-04 | Applera Corporation | Thermal-cycling pipette tip |
| JP4878601B2 (ja) * | 2005-10-13 | 2012-02-15 | 日水製薬株式会社 | 試験デバイス |
| CN103622926A (zh) | 2005-11-02 | 2014-03-12 | 帝国制药美国公司 | 感官上可接受的布洛芬口服给药制剂、制备和使用它的方法 |
| US8900828B2 (en) | 2006-05-01 | 2014-12-02 | Cepheid | Methods and apparatus for sequential amplification reactions |
| US8119352B2 (en) | 2006-06-20 | 2012-02-21 | Cepheld | Multi-stage amplification reactions by control of sequence replication times |
| US8187557B2 (en) | 2006-07-13 | 2012-05-29 | Cepheid | Reagent reservoir system for analytical instruments |
| JP3126208U (ja) * | 2006-08-04 | 2006-10-19 | 株式会社島津製作所 | 示差屈折率検出器及び液体クロマトグラフ。 |
| US20080108122A1 (en) | 2006-09-01 | 2008-05-08 | State of Oregon acting by and through the State Board of Higher Education on behalf of Oregon | Microchemical nanofactories |
| US20090093374A1 (en) * | 2007-01-25 | 2009-04-09 | Kahp-Yang Suh | Method of arraying cells at single-cell level inside microfluidic channel and method of analysing cells using the same, and cell analysis chip used for carrying out the same |
| EP2152893B1 (en) | 2007-06-11 | 2014-02-26 | Wako Pure Chemical Industries, Ltd. | Method for nucleic acid amplification using a microchip PCR with integrated real-time CE detection |
| NL1036865C2 (en) | 2009-04-16 | 2010-10-19 | Maxi Miliaan Bv | Child vehicle seat. |
| JP5881936B2 (ja) * | 2009-04-20 | 2016-03-09 | ソニー株式会社 | 試料溶液導入キット及び試料溶液注入器 |
| US20130004967A1 (en) * | 2009-11-23 | 2013-01-03 | Halverson Kurt J | Microwell array articles and methods of use |
| DE102010041478A1 (de) | 2010-09-27 | 2012-03-29 | Zumtobel Lighting Gmbh | Anordnung zur gerichteten Lichtabgabe |
| EP2441520A1 (en) | 2010-10-12 | 2012-04-18 | Eppendorf AG | Real-time amplification and micro-array based detection of nucleic acid targets in a flow chip assay |
| US8968684B2 (en) * | 2011-04-28 | 2015-03-03 | Bin Lian | Microplates, reaction modules and detection systems |
| US9095852B2 (en) * | 2011-08-22 | 2015-08-04 | The Regents Of The University Of California | Multilayer high density microwells |
| GB201118774D0 (en) * | 2011-10-31 | 2011-12-14 | British American Tobacco Co | Fluid distribution in an exposure device |
| US9808798B2 (en) * | 2012-04-20 | 2017-11-07 | California Institute Of Technology | Fluidic devices for biospecimen preservation |
| US9914968B2 (en) | 2012-09-26 | 2018-03-13 | Cepheid | Honeycomb tube |
| WO2015116627A1 (en) * | 2014-01-29 | 2015-08-06 | Arizona Board Of Regents On Behalf Of Arizona State University | Microreactor array platform |
| WO2015143442A2 (en) * | 2014-03-21 | 2015-09-24 | The Trustees Of Columbia University In The City Of New York | Methods and devices for selection and isolation of aptamers |
| CA3029276C (en) * | 2016-10-05 | 2023-03-07 | Abbott Laboratories | Devices and methods for sample analysis |
| JP7575189B2 (ja) * | 2017-11-17 | 2024-10-29 | Toppanホールディングス株式会社 | 標的分子の検出方法 |
-
2013
- 2013-03-15 US US13/843,739 patent/US9914968B2/en active Active
- 2013-09-26 KR KR1020157010847A patent/KR102214419B1/ko active Active
- 2013-09-26 MX MX2015003859A patent/MX363399B/es unknown
- 2013-09-26 US US14/431,259 patent/US10190165B2/en active Active
- 2013-09-26 CN CN201380062086.4A patent/CN105051546A/zh active Pending
- 2013-09-26 CN CN202010734994.5A patent/CN111979092A/zh active Pending
- 2013-09-26 BR BR112015006566-0A patent/BR112015006566B1/pt not_active IP Right Cessation
- 2013-09-26 JP JP2015534674A patent/JP6525877B2/ja active Active
- 2013-09-26 AU AU2013323428A patent/AU2013323428B2/en active Active
- 2013-09-26 AP AP2015008394A patent/AP2015008394A0/xx unknown
- 2013-09-26 EA EA201500369A patent/EA031989B1/ru not_active IP Right Cessation
- 2013-09-26 CA CA2886484A patent/CA2886484C/en active Active
- 2013-09-26 WO PCT/US2013/062042 patent/WO2014052671A1/en not_active Ceased
-
2015
- 2015-04-13 ZA ZA2015/02463A patent/ZA201502463B/en unknown
-
2018
- 2018-02-06 US US15/890,154 patent/US10870884B2/en active Active
- 2018-12-21 US US16/230,458 patent/US10767226B2/en active Active
-
2019
- 2019-05-07 JP JP2019087554A patent/JP2019141092A/ja active Pending
-
2020
- 2020-08-13 US US16/992,392 patent/US11739383B2/en active Active
- 2020-11-24 US US17/103,688 patent/US11795506B2/en active Active
-
2023
- 2023-08-07 US US18/350,916 patent/US12281358B2/en active Active
- 2023-10-04 US US18/481,156 patent/US12203135B2/en active Active
-
2024
- 2024-12-12 US US18/979,234 patent/US20250243541A1/en active Pending
-
2025
- 2025-03-14 US US19/079,552 patent/US20250327123A1/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20060263242A1 (en) | 2005-04-28 | 2006-11-23 | Fuji Photo Film Co., Ltd. | Microchip and analysis method using the same |
| JP2011506998A (ja) | 2007-12-17 | 2011-03-03 | ハイ−チング ゴング | マイクロ流体デバイス |
Also Published As
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US12281358B2 (en) | Honeycomb tube | |
| CN101679932A (zh) | 用于热交换化学过程的基于数字微流体的装置 | |
| JP2008148690A (ja) | マイクロチップを用いた核酸増幅方法およびマイクロチップ、それを用いた核酸増幅システム | |
| US20220032294A1 (en) | System and self-metering cartridges for point of care bioassays | |
| US20230043483A1 (en) | High-level multiplexing reaction vessel, reagent spotting device and associated methods | |
| Brennan et al. | Incorporating asymmetric PCR and microarray hybridization protocols onto an integrated microfluidic device, screening for the Escherichia coli ssrA gene | |
| US20230193366A1 (en) | System and self-metering cartridges for point of care bioassays | |
| JP5505646B2 (ja) | 生体試料定量方法 | |
| CN113801925A (zh) | 一种收纳定量化液滴pcr中油包水乳液容器及其使用方法 | |
| US12428674B2 (en) | System and self-metering cartridges for point of care bioassays | |
| CN113755563B (zh) | 一种使用微液滴进行核酸分子定量的方法及定量化系统 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PA0105 | International application |
Patent event date: 20150424 Patent event code: PA01051R01D Comment text: International Patent Application |
|
| PG1501 | Laying open of application | ||
| A201 | Request for examination | ||
| PA0201 | Request for examination |
Patent event code: PA02012R01D Patent event date: 20180920 Comment text: Request for Examination of Application |
|
| E902 | Notification of reason for refusal | ||
| PE0902 | Notice of grounds for rejection |
Comment text: Notification of reason for refusal Patent event date: 20190813 Patent event code: PE09021S01D |
|
| E90F | Notification of reason for final refusal | ||
| PE0902 | Notice of grounds for rejection |
Comment text: Final Notice of Reason for Refusal Patent event date: 20200616 Patent event code: PE09021S02D |
|
| E701 | Decision to grant or registration of patent right | ||
| PE0701 | Decision of registration |
Patent event code: PE07011S01D Comment text: Decision to Grant Registration Patent event date: 20201123 |
|
| GRNT | Written decision to grant | ||
| PR0701 | Registration of establishment |
Comment text: Registration of Establishment Patent event date: 20210203 Patent event code: PR07011E01D |
|
| PR1002 | Payment of registration fee |
Payment date: 20210203 End annual number: 3 Start annual number: 1 |
|
| PG1601 | Publication of registration | ||
| PR1001 | Payment of annual fee |
Payment date: 20231218 Start annual number: 4 End annual number: 4 |
|
| PR1001 | Payment of annual fee |
Payment date: 20250114 Start annual number: 5 End annual number: 5 |