KR102111178B1 - 엔도뉴클레아제 - Google Patents

엔도뉴클레아제 Download PDF

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KR102111178B1
KR102111178B1 KR1020147024472A KR20147024472A KR102111178B1 KR 102111178 B1 KR102111178 B1 KR 102111178B1 KR 1020147024472 A KR1020147024472 A KR 1020147024472A KR 20147024472 A KR20147024472 A KR 20147024472A KR 102111178 B1 KR102111178 B1 KR 102111178B1
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endonuclease
vsenda
dna
sample
inactivation
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Korean (ko)
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KR20140135962A (ko
Inventor
오라브 라네스
린다 하브다렌
테레세 솔스타드
마리트 로렌트젠
브존 알터마크
인가 레이로스
론니 헬란드
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바이오텍 파마콘 에이에스에이
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Priority claimed from GBGB1202768.6A external-priority patent/GB201202768D0/en
Priority claimed from GBGB1216029.7A external-priority patent/GB201216029D0/en
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Publication of KR20140135962A publication Critical patent/KR20140135962A/ko
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/21—Endodeoxyribonucleases producing 5'-phosphomonoesters (3.1.21)
    • C12Y301/21001—Deoxyribonuclease I (3.1.21.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/30—Phosphoric diester hydrolysing, i.e. nuclease
    • C12Q2521/301—Endonuclease

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Immunology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
KR1020147024472A 2012-02-17 2013-02-18 엔도뉴클레아제 Active KR102111178B1 (ko)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
GBGB1202768.6A GB201202768D0 (en) 2012-02-17 2012-02-17 Endonucleases
GB1202768.6 2012-02-17
GBGB1216029.7A GB201216029D0 (en) 2012-09-07 2012-09-07 Endonucleases
GB1216029.7 2012-09-07
PCT/GB2013/050387 WO2013121228A1 (en) 2012-02-17 2013-02-18 Endonucleases

Publications (2)

Publication Number Publication Date
KR20140135962A KR20140135962A (ko) 2014-11-27
KR102111178B1 true KR102111178B1 (ko) 2020-05-15

Family

ID=47741183

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1020147024472A Active KR102111178B1 (ko) 2012-02-17 2013-02-18 엔도뉴클레아제

Country Status (12)

Country Link
US (2) US9422595B2 (https=)
EP (1) EP2814954B1 (https=)
JP (2) JP6259775B2 (https=)
KR (1) KR102111178B1 (https=)
CN (1) CN104245928B (https=)
AU (1) AU2013220102A1 (https=)
CA (1) CA2863756C (https=)
DK (1) DK2814954T3 (https=)
ES (1) ES2622906T3 (https=)
IN (1) IN2014MN01822A (https=)
LT (1) LT2814954T (https=)
WO (1) WO2013121228A1 (https=)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB201414745D0 (en) 2014-08-19 2014-10-01 Articzymes As Exonucleases
US11246905B2 (en) 2016-08-15 2022-02-15 President And Fellows Of Harvard College Treating infections using IdsD from Proteus mirabilis
CN109207563A (zh) * 2017-06-30 2019-01-15 青岛科技大学 一种lamp防污染的方法
WO2020227415A1 (en) * 2019-05-06 2020-11-12 President And Fellows Of Harvard College Dna-degrading proteins and uses thereof
CN112176028B (zh) * 2019-07-05 2024-04-16 深圳华大生命科学研究院 一种基于核酸内切酶的快速wgs建库方法
PL431144A1 (pl) 2019-09-13 2021-03-22 Blirt Spółka Akcyjna Nowa, niespecyficzna termolabilna nukleaza aktywna w niskiej temperaturze, szerokim zakresie pH oraz wysokich stężeniach soli
WO2022248874A1 (en) * 2021-05-27 2022-12-01 Lumiradx Uk Ltd Improvements in or relating to digestion of reaction products
KR20240134996A (ko) 2022-01-20 2024-09-10 씨-렉타 게엠베하 향상된 내염성 및/또는 온도 성능을 가지는 핵산분해효소
CN119351373A (zh) * 2023-07-24 2025-01-24 北京志道生物科技有限公司 一种核酸内切酶及其应用
CN118165957B (zh) * 2024-03-18 2025-06-13 北京石油化工学院 一种重组核酸酶及其应用
WO2025227034A1 (en) * 2024-04-26 2025-10-30 New England Biolabs, Inc. Salt active nuclease compositions and methods
EP4692336A1 (en) 2024-08-08 2026-02-11 Arcticzymes AS Purification of bionanoparticles
CN120173915B (zh) * 2025-05-21 2025-08-26 翌圣生物科技(上海)股份有限公司 一种全能核酸酶突变体及其应用

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020172972A1 (en) 2001-05-15 2002-11-21 President And Fellows Of Harvard College Use of a selectively inactivatable enzyme to digest contaminating nucleic acid
US20080160528A1 (en) 2005-03-02 2008-07-03 Lorenz Michael G Use of Nucleases for Degrading Nucleic Acids in the Presence of Chaotropic Agents and/or Surfactants
US20090047705A1 (en) 2005-03-08 2009-02-19 Takara Bio Inc. Microorganism-derived psychrophilic endonuclease
WO2011010094A1 (en) 2009-07-21 2011-01-27 Biotec Pharmacon Asa A method of removing nucleic acid contamination in reverse transcription and amplification reactions

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5006472A (en) 1988-06-03 1991-04-09 Miles Inc. Enzymatic purification process
US5700672A (en) 1992-07-23 1997-12-23 Stratagene Purified thermostable pyrococcus furiousus DNA ligase
GB9716664D0 (en) 1997-08-06 1997-10-15 Norwegian Inst Of Fisheries & A method of removing nucleic acid contamination reactions
US20080044851A1 (en) 2006-06-02 2008-02-21 Epicentre Technologies Compositions and methods for removal of DNA from a sample
WO2007142306A1 (ja) 2006-06-08 2007-12-13 Agave Inc. 高水溶性アガベ・イヌリン、アガベ・イヌリン含有製品、アガベ・イヌリン由来製品、副産物及びそれらの製法
US20110195486A1 (en) 2008-05-23 2011-08-11 Life Technologies Corporation Methods for removing nucleic acid contamination from reagents

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020172972A1 (en) 2001-05-15 2002-11-21 President And Fellows Of Harvard College Use of a selectively inactivatable enzyme to digest contaminating nucleic acid
US20080160528A1 (en) 2005-03-02 2008-07-03 Lorenz Michael G Use of Nucleases for Degrading Nucleic Acids in the Presence of Chaotropic Agents and/or Surfactants
US20090047705A1 (en) 2005-03-08 2009-02-19 Takara Bio Inc. Microorganism-derived psychrophilic endonuclease
WO2011010094A1 (en) 2009-07-21 2011-01-27 Biotec Pharmacon Asa A method of removing nucleic acid contamination in reverse transcription and amplification reactions

Also Published As

Publication number Publication date
US9650618B2 (en) 2017-05-16
DK2814954T3 (en) 2017-05-15
AU2013220102A1 (en) 2014-08-21
IN2014MN01822A (https=) 2015-07-03
KR20140135962A (ko) 2014-11-27
US9422595B2 (en) 2016-08-23
EP2814954A1 (en) 2014-12-24
JP2015507927A (ja) 2015-03-16
CA2863756A1 (en) 2013-08-22
WO2013121228A1 (en) 2013-08-22
CA2863756C (en) 2018-03-06
US20160355798A1 (en) 2016-12-08
CN104245928B (zh) 2017-08-18
JP2018038414A (ja) 2018-03-15
US20140370514A1 (en) 2014-12-18
JP6259775B2 (ja) 2018-01-10
CN104245928A (zh) 2014-12-24
LT2814954T (lt) 2017-05-10
ES2622906T3 (es) 2017-07-07
EP2814954B1 (en) 2017-04-05

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