KR102102253B1 - Skin external composition having excellent bactericidal activities - Google Patents

Abstract

The present invention relates to a composition for external application for skin having excellent antiseptic power without using a chemical preservative, and more specifically, xylityl caprylate having excellent antiseptic power; And ethyl hexyl glycerin, glyceryl caprylate, p-anisic acid, and citrus mixed extracts, thereby providing a composition for external application for skin having excellent antiseptic power through a synergistic effect on the antiseptic power of these materials. .

Description

Skin external composition having excellent bactericidal activities}

The present invention relates to a composition for external application for skin having excellent antiseptic power without using a chemical preservative, and more specifically, xylityl caprylate having excellent antiseptic power; And ethylhexyl glycerin, glyceryl caprylate, p-anisic acid, and citrus mixed extracts, and one or more substances selected from the mixture, thereby providing a composition for external application for skin having excellent antiseptic power through synergistic effect on the antiseptic power of these substances. will be.

The composition for external application for skin is caused by falling bacteria in the manufacturing site, contamination by microorganisms attached to the manufacturing water or raw material transfer piping, etc., contamination by skin on the user's fingers, palms, or face during use, or bacteria on the surface of product containers, or It may be contaminated with microorganisms due to mixing of foreign substances during use. For this reason, in the composition for external application, the purpose is to suppress the growth of contaminated microorganisms and to improve the antiseptic power to kill contaminated microorganisms. Paraoxybenzoic acid esters (parabens), imidazolidinylurea, phenoxyethanol, chlorophenesin Preservatives such as benzoic acid, sorbic acid, and dehydroacetic acid are blended. However, these chemical preservatives have excellent effectiveness as an antiseptic means used in the composition for external application for skin, but have disadvantages such as toxicity, skin irritation, irritation, and allergies, thereby reducing the use of chemical preservatives or alternative substances of such chemical preservatives. There is a demand for

Of course, a method for preparing a composition for external application for skin without mixing a conventional chemical preservative using diols, which have recently been known for its antibacterial activity, or for packaging in a container that prevents contamination of microorganisms and suppresses contamination of microorganisms in the manufacturing process It is also possible to prepare an external preparation for skin that does not use preservatives or has a lower preservative content than the normal level. However, in the case of Dior, skin irritation affects safety, solubilization, and stability of the emulsion formulation, and when using a container that suppresses sterile production or fungal inflow, the manufacturing process is complicated and equipment maintenance costs and container costs increase. Economical versatility is poor.

Japanese Patent Publication No. 2010-270083 (Publication date: 2010.12.02)

Accordingly, the present inventors tried to provide a composition for external application for skin having a safe antiseptic power from harmful microorganisms introduced into the skin, at least equal to the composition for external application for skin using chemical preservatives without using a conventional chemical preservative, and ethyl xylyl caprylate. Hexyl glycerin, glyceryl caprylate, p-anisic acid, and citrus mixtures are used in combination with one or more substances selected from the extract to find that they can provide excellent antiseptic power due to the synergistic effect on the antiseptic power of these materials. The present invention has been completed.

Accordingly, an object of the present invention is to provide a composition for external application for skin having excellent antiseptic power without using a conventional chemical preservative.

In order to achieve the above object, the present invention is xylyl caprylate; And ethylhexyl glycerin, glyceryl caprylate, p-anisic acid and citrus mixed extract, at least one material selected from the present invention.

The present invention provides a preservative power equal to or greater than that of the case of using a chemical preservative without using a conventional chemical preservative, and is safe to use on the skin because it does not cause toxicity, skin irritation, irritation, and allergies due to the use of the chemical preservative. You can.

1 is a flow chart showing a procedure for evaluating antiseptic force.

The present invention is xylyl caprylate; And ethyl hexyl glycerin, glyceryl caprylate, p-anisic acid, and citrus mixed extract, and the composition is used in combination with one or more substances selected from the present invention.

In the present invention, xylyl caprylate having an antibacterial effect; And glyceryl caprylate, p-anisic acid, and citrus mixed extract, by using one or more substances selected in combination, thereby providing a synergistic effect and providing excellent antiseptic power even in a small amount.

The composition of the present invention is 0.01 to 4.0% by weight of xylyl caprylate, 0.01 to 1.0% by weight of ethylhexyl glycerin, 0.01 to 1.0% by weight of glyceryl caprylate, 0.01 to p-anisic acid, based on the total weight of the composition. 1.0% by weight, may contain a citrus mixed extract in an amount of 0.01 to 1.0% by weight.

In addition, the composition of the present invention is preferably xylyl caprylate 1.0 wt%, ethylhexylglycerin 0.05 to 0.1 wt%, glyceryl caprylate 0.1 to 0.2 wt%, p-anisic acid, based on the total weight of the composition. Although 0.1 to 0.2% by weight and the citrus mixed extract may be appropriately mixed in the range of 0.05 to 0.2% by weight, the range of the mixing ratio is not limited thereto.

Citrus mixed extract used in the present invention is an extract of citrus plants, but is not limited to citrus plants that can be used, but is not limited to citrus (Citrus Paradisi), bergamot (Citrus Aurantium Bergamia), sweet orange, tangerine (Citrus Tangerina), mandarin orange Extracts or mixtures thereof may be used, and in the present invention, extracts from all parts of citrus fruits such as stems, leaves, flowers, and fruits may be used, and the extract is not limited to any specific part. In addition, the extract includes not only the leachate obtained by leaching and transferring from citrus plants, but also the concentrate obtained by partially or fully condensing the leachate or those prepared by drying the concentrate again, preferably the extract prepared as such. Used in combination with vegetable glycerin. In addition, in the present invention, as an extract, a fruit, leaf, stem, etc. of a citrus plant is squeezed and mixed to ferment, and a fermented product is centrifuged to take a liquid fermentation extract, followed by heat treatment and mixing with vegetable glycerin. It is desirable to do.

As described above, the present invention uses xylyl caprylate and one or more substances selected from ethyl hexylglycerin, glyceryl caprylate, p-anisic acid, and citrus mixed extract. It is possible to provide a composition for external application for skin having sufficient antiseptic power without mixing a chemical preservative or an existing diol having the potential to cause skin irritation or irritation.

Hereinafter, the contents of the present invention will be described in more detail through examples and test examples. These examples and test examples are presented to understand the contents of the present invention, but the scope of the present invention is not limited to them.

[Reference Example 1] Preparation of Examples 1 to 11 and Comparative Examples 1 to 7

It was prepared as a formulation of a lotion in a conventional manner according to the composition of Tables 1 to 2 (unit: weight%). At this time, the citrus mixed extract used a Bio Citro liquid (Quinabra) product, and the products include grapefruit fruit extract, bergamot fruit extract, orange (Citrus Aurantium Dulcis) fruit extract, tangerine fruit extract, and glycerin.

Raw material name Example One 2 3 4 5 6 7 8 9 10 11 Purified water Balance Balance Balance Balance Balance Balance Balance Balance Balance Balance Balance Cyclopentasiloxane 3.00 3.00 3.00 3.00 3.00 3.00 3.00 3.00 3.00 3.00 3.00 Dipropylene glycol 2.50 2.50 2.50 2.50 2.50 2.50 2.50 2.50 2.50 2.50 2.50 Dimethicone / vinyl dimethicone cross polymer 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 Hydrolyzed wheat protein / fibropic polymer 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 Beta-glucan 1.30 1.30 1.30 1.30 1.30 1.30 1.30 1.30 1.30 1.30 1.30 PG-100 Stearate 1.20 1.20 1.20 1.20 1.20 1.20 1.20 1.20 1.20 1.20 1.20 Cetylethylhexanoate 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 Dimethicone 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 Cetearyl alcohol 0.50 0.50 0.50 0.50 0.50 0.50 0.50 0.50 0.50 0.50 0.50 Silica 0.50 0.50 0.50 0.50 0.50 0.50 0.50 0.50 0.50 0.50 0.50 Ammonium acryloyl dimethyl taurate / VP copolymer 0.40 0.40 0.40 0.40 0.40 0.40 0.40 0.40 0.40 0.40 0.40 Sorbitan sesquioleate 0.30 0.30 0.30 0.30 0.30 0.30 0.30 0.30 0.30 0.30 0.30 Carbomer 0.15 0.15 0.15 0.15 0.15 0.15 0.15 0.15 0.15 0.15 0.15 Triethanolamine 0.14 0.14 0.14 0.14 0.14 0.14 0.14 0.14 0.14 0.14 0.14 Disodium LED 0.02 0.02 0.02 0.02 0.02 0.02 0.02 0.02 0.02 0.02 0.02 Coloring Proper Proper Proper Proper Proper Proper Proper Proper Proper Proper Proper Spices Proper Proper Proper Proper Proper Proper Proper Proper Proper Proper Proper Xylyl caprylate 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 Ethylhexylglycerin 0.1 - - - 0.05 0.05 0.05 - - 0.04 0.025 Glyceryl caprylate - 0.1 - - 0.05 - - 0.05 0.05 0.03 0.025 p-anisic acid - - 0.1 - - 0.05 - 0.05 - 0.03 0.025 Citrus Blend Extract - - - 0.1 - - 0.05 - 0.05 - 0.025 1.2-nucleic acid diol - - - - - - - - - - - Phenoxyethanol - - - - - - - - - - -

Raw material name Comparative example One 2 3 4 5 6 7 Purified water Balance Balance Balance Balance Balance Balance Balance Cyclopentasiloxane 3.00 3.00 3.00 3.00 3.00 3.00 3.00 Dipropylene glycol 2.50 2.50 2.50 2.50 2.50 2.50 2.50 Dimethicone / vinyl dimethicone cross polymer 2.00 2.00 2.00 2.00 2.00 2.00 2.00 Hydrolyzed wheat protein / fibropic polymer 2.00 2.00 2.00 2.00 2.00 2.00 2.00 Beta-glucan 1.30 1.30 1.30 1.30 1.30 1.30 1.30 PG-100 Stearate 1.20 1.20 1.20 1.20 1.20 1.20 1.20 Cetylethylhexanoate 1.00 1.00 1.00 1.00 1.00 1.00 1.00 Dimethicone 1.00 1.00 1.00 1.00 1.00 1.00 1.00 Cetearyl alcohol 0.50 0.50 0.50 0.50 0.50 0.50 0.50 Silica 0.50 0.50 0.50 0.50 0.50 0.50 0.50 Ammonium acryloyl dimethyl taurate /
VP copolymer 0.40 0.40 0.40 0.40 0.40 0.40 0.40 Sorbitan sesquioleate 0.30 0.30 0.30 0.30 0.30 0.30 0.30 Carbomer 0.15 0.15 0.15 0.15 0.15 0.15 0.15 Triethanolamine 0.14 0.14 0.14 0.14 0.14 0.14 0.14 Disodium LED 0.02 0.02 0.02 0.02 0.02 0.02 0.02 Coloring Proper Proper Proper Proper Proper Proper Proper Spices Proper Proper Proper Proper Proper Proper Proper Xylyl caprylate - 1.00 - - - - - Ethylhexylglycerin - - 0.2 - - - - Glyceryl caprylate - - - 0.4 - - - p-anisic acid - - - - 0.4 - - Citrus Blend Extract - - - - - 0.4 - 1.2-nucleic acid diol - - - - - - 1.0 Phenoxyethanol - - - - - - 0.4

 [Test Example 1] Antiseptic force test

In order to evaluate the antiseptic force, the test was basically performed according to the procedure shown in FIG. 1.

E. coli in 20 to 30 g of the cosmetics of Examples 1 to 11 and Comparative Examples 1 to 7 in which the initial microorganisms were removed.Escherichia coli; ATCC 8739), Staphylococcus aureus (Staphlyococcus aureus; ATCC 6538), Pseudomonas aeruginosa (Pseudomonas aeruginosa; ATCC 99027) and Candida (Candida albicans ATCC 10231), etc.⁵cfu (colony forming unit, colony forming unit) / g was added and mixed. While incubating them in a 32 ° C constant temperature bath for 4 weeks, 1 g of each cosmetic was taken at intervals of 7, 14, and 28 days to measure the number of viable cells, and the results are shown in Table 3 below.

In addition, in the case of fungi, the initial concentration of 1 x 10 ⁵ cfu / g per sample was added to 20 to 30 g of the cosmetic compositions of Examples 1 to 11 and Comparative Examples 1 to 7, aspergillus niger (ATCC 16404). After adding and mixing as much as possible, 1 g of each cosmetic was taken at intervals of 7, 14, and 28 days while incubating in a 25 ° C. bath to measure the number of viable cells and observe the presence or absence of hyphae and spores on the sample surface, and the results are shown in Table 4 below. It is shown in

Cosmetic Bacteria (cfu / g) Initial bacteria count Day 7 Day 14 Day 28 Lotion Example 1 5 × 10 ⁵ <1 × 10 ³ <1 × 10 ² <1 × 10 ² Example 2 5 × 10 ⁵ <1 × 10 ³ <1 × 10 ² <1 × 10 ² Example 3 5 × 10 ⁵ <1 × 10 ³ <1 × 10 ² <1 × 10 ² Example 4 5 × 10 ⁵ <1 × 10 ³ <1 × 10 ² <1 × 10 ² Example 5 5 × 10 ⁵ <1 × 10 ³ <1 × 10 ² <1 × 10 ² Example 6 5 × 10 ⁵ <1 × 10 ³ <1 × 10 ² <1 × 10 ² Example 7 5 × 10 ⁵ 1 × 10 ³ <1 × 10 ² <1 × 10 ² Example 8 5 × 10 ⁵ 1 × 10 ³ <1 × 10 ² <1 × 10 ² Example 9 5 × 10 ⁵ <1 × 10 ³ <1 × 10 ² <1 × 10 ² Example 10 5 × 10 ⁵ <1 × 10 ³ <1 × 10 ² <1 × 10 ² Example 11 5 × 10 ⁵ <1 × 10 ³ <1 × 10 ² <1 × 10 ² Comparative Example 1 5 × 10 ⁵ > 1 × 10 ⁶ > 1 × 10 ⁶ > 1 × 10 ⁶ Comparative Example 2 5 × 10 ⁵ > 1 × 10 ⁶ > 1 × 10 ⁶ > 1 × 10 ⁶ Comparative Example 3 5 × 10 ⁵ 4.5 × 10 ⁵ 1 × 10 ⁵ 2 × 10 ³ Comparative Example 4 5 × 10 ⁵ 3.5 × 10 ⁵ 1 × 10 ⁵ 3 × 10 ⁴ Comparative Example 5 5 × 10 ⁵ 3.5 × 10 ⁵ 1 × 10 ⁵ 1 × 10 ⁴ Comparative Example 6 5 × 10 ⁵ 4 × 10 ⁵ 4 × 10 ⁵ 4 × 10 ⁵ Comparative Example 7 5 × 10 ⁵ <1 × 10 ³ <1 × 10 ² <1 × 10 ²

Cosmetic Mold (cfu / g) Initial bacteria count Day 7 Day 14 Day 28 Visual observation Lotion Example 1 1 × 10 ⁵ 1 × 10 ⁴ 2 × 10 ² 1 × 10 ² nothing strange Example 2 1 × 10 ⁵ 1 × 10 ⁴ 2 × 10 ³ 1 × 10 ³ nothing strange Example 3 1 × 10 ⁵ 1 × 10 ⁴ 1 × 10 ³ 1 × 10 ² nothing strange Example 4 1 × 10 ⁵ 1 × 10 ⁴ 1 × 10 ³ 1 × 10 ³ nothing strange Example 5 1 × 10 ⁵ 1 × 10 ⁴ 1 × 10 ³ 1 × 10 ³ nothing strange Example 6 1 × 10 ⁵ 1 × 10 ⁴ 1 × 10 ³ 1 × 10 ³ nothing strange Example 7 1 × 10 ⁵ 1 × 10 ⁴ 1 × 10 ³ 1 × 10 ³ nothing strange Example 8 1 × 10 ⁵ 1 × 10 ⁴ 1 × 10 ³ 1 × 10 ³ nothing strange Example 9 1 × 10 ⁵ 1 × 10 ⁴ 1 × 10 ³ 1 × 10 ³ nothing strange Example 10 1 × 10 ⁵ 1 × 10 ⁴ 1 × 10 ³ 1 × 10 ³ nothing strange Example 11 1 × 10 ⁵ 1 × 10 ⁴ 1 × 10 ³ 1 × 10 ³ nothing strange Comparative Example 1 1 × 10 ⁵ 1 × 10 ⁵ 1 × 10 ⁵ 1 × 10 ⁵ Sporulation Comparative Example 2 1 × 10 ⁵ 2.3 × 10 ⁴ 3.6 × 10 ⁴ 1 × 10 ³ nothing strange Comparative Example 3 1 × 10 ⁵ 3 × 10 ⁴ 2.1 × 10 ⁴ 1 × 10 ³ nothing strange Comparative Example 4 1 × 10 ⁵ 3 × 10 ⁴ 2.1 × 10 ⁴ 1 × 10 ³ nothing strange Comparative Example 5 1 × 10 ⁵ 1 × 10 ³ 1 × 10 ² 1 × 10 ² nothing strange Comparative Example 6 1 × 10 ⁵ 1 × 10 ⁵ 1 × 10 ⁵ 1 × 10 ⁵ Sporulation Comparative Example 7 1 × 10 ⁵ 1 × 10 ⁴ 2 × 10 ³ 1 × 10 ³ nothing strange

Looking at Tables 3 and 4, in the case of Examples 1 to 11 according to the present invention, Comparative Example 2, in which xylyl caprylate and ethylhexylglycerin glyceryl caprylate, p-anisic acid, and citrus mixed extract were used respectively It can be observed that there is a better antiseptic force even in the use of less than ~ 6, compared with the case of Comparative Example 7 using the previously used 1,2-hexanediol and phenoxyethanol, the use of less amount It can also be seen that it exhibits a better degree of antiseptic power.

[Test Example 2] Skin irritation test

A patch test was performed on 40 subjects for 24 hours to evaluate skin safety in the human body of xylyl caprylate, ethylhexylglycerin, glyceryl caprylate, p-anisic acid, and citrus mixed extract. The patch was attached to the upper part of the subject's back, and 20 μl of the prepared test substance was applied using an IQ chamber (chemotechique, Sweeden). After attaching the patch and removing the patch after 24 hours, the first reading was performed 30 minutes later, and the second reading was performed after 24 hours. In order to find out the intensity of skin irritation of the sample, weights were given according to the degree of positive reactions of the skin, and the skin responsiveness of the sample was determined according to Equation 1 below, and the skin irritation of the sample was determined.

<Criteria for Judgment>

Score less than 1 (Class I): No irritation range

1 to 3 (Class II): Light stimulation

3 or more and less than 5 (grade III): medium irritation

5 or higher (grade IV): strong stimulation

Test substance score Xylyl caprylate 10% 0.90 Ethylhexylglycerin 3% 0.31 Glyceryl caprylate 5% 0.94 p-anisic acid 1% 0.94 Citrus Blend Extract 1% 0.00

In the results of Table 5, it can be confirmed that xylyl caprylate, ethylhexylglycerin, glyceryl caprylate, p-anisic acid, and citrus mixed extract are all irritated.

Therefore, it can be seen that even if the composition containing these substances is used as an external preparation for skin, it can be safely used without causing irritation to the skin.

Claims (4)

Xylyl caprylate; And one or more substances selected from ethyl hexyl glycerin, glyceryl caprylate, p-anisic acid, and citrus mixed extract;
The xylyl caprylate is 0.01 to 4.0 wt% based on the total weight of the composition, and one or more substances selected from the ethyl hexyl glycerin, glyceryl caprylate, p-anisic acid and citrus mixed extract are 0.01 to 1.0 wt% in total. The composition for external application for skin contained in an amount of. According to claim 1, wherein the xylyl caprylate relative to the total weight of the composition is 0.01 to 1.0% by weight, the ethyl hexyl glycerin, glyceryl caprylate, p-anisic acid and citrus mixed extract is one or more substances selected from the total amount As a composition for external application for skin contained in an amount of 0.01 to 0.1% by weight. According to claim 1 or claim 2, The xylyl caprylate; And one or more substances selected from the ethylhexyl glycerin, glyceryl caprylate, p-anisic acid, and citrus mixed extract, in a weight ratio of 10: 1. The composition for topical skin application as claimed in claim 1, wherein the composition has improved antiseptic properties.

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