KR101356799B1 - Natural preservative composition containing the extract of Tabebuia avellanedae, Smilax chian L., Rumex japonicus and Agastache rugosa - Google Patents

Abstract

The present invention relates to a natural complex preservative composition containing the Tahibo extract, Tobokyeong extract, Banga leaf extract and Yangje-Geun extract.
The natural composite preservative composition of the present invention is not toxic to the human body and has an excellent antiseptic and antimicrobial effect, and can be used as a natural preservative in cosmetics, foods and medicines instead of synthetic preservatives.

Description

Natural preservative composition containing the extract of Tabebuia avellanedae, Smilax chian L., Rumex japonicus and Agastache rugosa}

The present invention relates to a natural complex preservative composition containing the Tahibo extract, Tobokyeong extract, Banga leaf extract and Yangje-Geun extract.

Preservatives are essential to maintain the quality of various products such as cosmetics, medicines, household goods, and foods for a long time. Especially, the preservatives have a long shelf life and consist mainly of oil or water, and they are nitrogen sources such as glycerin or sorbitol, which are carbon sources of microorganisms. In the case of cosmetics composed of amino acid derivatives, proteins and the like is more essential.

Cosmetics generally have a shelf life of 1 to 3 years at room temperature and are very vulnerable to microorganisms due to continuous contact with hands after opening. Therefore, high preservatives should be used to block the contamination from these microorganisms and to ensure the safety and quality of the product.

However, conventional cosmetic preservatives are mostly chemical preservatives prepared by chemical methods. These chemical preservatives are stable to the human body due to problems such as promoting skin aging, skin irritation, carcinogenesis, and endocrine disruption and lack of biodegradation when used in human skin for a long time. Development is needed.

In addition, typical preservatives such as paraben (p-hydroxybenzoic acid ester), formaldehyde-donor and isothiazolinone are potential skin allergies and environmental hormones (Andera Countti et. al ., Contact Dermatitis , 37:35, 1997; Edwin meat al ., Toxicology and Applied Pharmacology , 153: 12, 1998) and the risks of rapid, chronic toxicity, and mutagenesis from persistent body scale are widely recognized and some producers are working to develop alternative preservatives in response to consumer needs.

Due to these problems, research on natural preservatives having excellent safety and economical efficiency of products is ongoing, and as a result, extracts of animal and vegetable origin such as spices, essential oils, and herbal medicines (Korean Patent Nos. 10-09862490, 10-07876340) No. 10-07825990, No. 10-09814070, No. 10-07298310, No. 10-0822416, No. 10-09428300, No. 2009-0025654, No. 2007-0080707 and No. 2009- 0075282) and natural antimicrobial agents of microbial origin such as bacteriocin have been reported.

However, many companies do not use natural materials as preservatives due to the high price of natural materials and lack of processing technology for natural materials. In addition, in the case of naturally active substances, most of them are not commercialized due to color, deterioration of stability, narrow antibacterial spectrum, problems in formulation, reproducibility, and the like.

Therefore, the present inventors have made efforts to develop a natural product extract having an antiseptic effect among various natural extracts, and when using a mixture of Tahibo extract, Tobokyeong extract, Banga leaf extract and Yangje Geun extract inhibits the growth of microorganisms compared to each natural extract It was confirmed that the effect was increased and the present invention was completed.

An object of the present invention is to provide a natural composite preservative composition containing Tahibo extract, Tobokyeong extract, Banga leaf extract and Yangje Geun extract excellent in natural antiseptic and antibacterial effect.

In order to achieve the above object, the present invention provides a natural complex preservative composition containing the Tahibo extract, Tobok-ryong extract, the fennel leaf extract and Yangje-Geun extract.

The present invention also provides a cosmetic containing the above-mentioned natural composite preservative composition as an antiseptic component.

The invention also. Provided is a food containing the above-mentioned natural composite preservative composition as an antiseptic component.

The natural composite preservative composition of the present invention is not toxic to the human body and has an excellent antiseptic and antimicrobial effect, and can be used as a natural preservative in cosmetics, foods and medicines instead of synthetic preservatives.

Figure 1 is a comparison of the microbial growth minimum inhibitory concentration average value and the microbial growth minimum inhibitory concentration of the natural complex preservative composition for each strain.

Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. In general, the nomenclature used herein is well known and commonly used in the art.

In a consistent aspect of the present invention, the present invention relates to a natural complex preservative composition containing Tahibo extract, Tobok Ryong extract, Bangah Leaf Extract and Yangje Geun Extract.

In the present invention, the content of Tahibo extract, Tobok-ryong extract, Bangalore extract and Yangje-geun extract may be characterized in that each of 0.01 to 2.0% by weight.

The natural complex preservative composition may preferably be composed of 0.2% by weight of tahibo extract, 0.05% by weight of Tobok-ryong extract, 0.05% by weight of leaf extract and 0.2% by weight of Yangje-Geun extract.

In an embodiment of the present invention, 100 g of dried Tahibo was pulverized, and then heated and refluxed with 70% (v / v) ethanol aqueous solution for 12 hours, cooled, and filtered through a filter paper having a permeation size of 1.2 μm to obtain a filtrate. The obtained filtrate was concentrated under reduced pressure and dried under reduced pressure to obtain 6.87 g of Tahibo extract.

In another embodiment of the present invention, 100 g of dried Tobok-ryeong was ground and refluxed with 70% (v / v) ethanol aqueous solution for 12 hours, refrigerated, and filtered through a filter paper having a permeation size of 1.2 μm to obtain a filtrate. . The filtrate obtained was concentrated under reduced pressure and dried under reduced pressure to obtain 9.65 g of Tobok Rin extract.

In another embodiment of the present invention, 100 g of dried dried leaf leaves were pulverized, heated and refluxed with 70% (v / v) ethanol aqueous solution for 12 hours, cooled, and filtered through a filter paper having a permeation size of 1.2 μm to obtain a filtrate. . The filtrate obtained was concentrated under reduced pressure and dried under a reduced pressure to obtain 5.31 g of leaf extract.

In another embodiment of the present invention, 100 g of dried dried Yangje root was pulverized, heated to reflux for 12 hours with 70% (v / v) ethanol aqueous solution, cooled and cooled, and filtered through a filter paper having a permeation size of 1.2 μm to obtain a filtrate. The obtained filtrate was concentrated under reduced pressure and dried under reduced pressure to obtain 16.5 g of Yangje Geun extract.

As a result of measuring the minimum inhibitory concentration by using a solid culture dilution method to measure the antimicrobial activity of the Tahibo extract, Tobokyeong extract, Banga leaf extract and Yangje-geun extract, in the case of Tahibo extract, Gram-positive bacteria Staphylococcus aureus and E. coli 0.1 It showed good antibacterial activity at the concentration of% by weight, but showed relatively weak antibacterial activity against P. aeruginosa, Gram-positive bacteria, and 0.2% by weight of Candida yeast. It showed relatively weak antimicrobial activity. In addition, the extracts of Tobok-ryeong showed good antimicrobial activity against Gram-negative bacteria E. coli and Pseudomonas aeruginosa at a concentration of 0.1% by weight, but showed relatively weak antimicrobial activity against Staphylococcus aureus, Candida yeast and Black Bacillus. In the case of M. leaf extract, it showed weak antimicrobial activity against both Gram-negative bacteria and Gram-positive bacteria, but showed effective antimicrobial activity at 0.05 wt% concentration against fungi yeast and filamentous fungi. In the case of Yangje-geun extract also showed antimicrobial activity against bacteria, it was confirmed that the antimicrobial activity against filamentous fungi was excellent (Table 1).

In another embodiment of the present invention by mixing the extracts in various ratios to prepare a natural composite preservative composition (Table 2), to determine the antiseptic power of the prepared natural composite preservative composition (paper disc method) and microorganisms As a result of evaluation of growth inhibition concentration, the natural complex preservative showed antiseptic activity in various combinations, mixed with 0.2% by weight of tahibo extract, 0.05% by weight of Tobokyeong extract, 0.05% by weight of leaf extract and 0.2% by weight of Yangje-Geun extract. It was confirmed that the natural composite preservative composition was an excellent combination (Table 3 and Table 4).

Tahibo extract, Tobokyeong extract, Banga leaf extract and Yangje Geun extract contained in the natural complex antiseptic composition has already been reported antibacterial and antiseptic effect, as in some embodiments of the present invention, each extract is Gram-positive bacteria, Gram-negative bacteria and fungi do not show excellent antimicrobial activity, so the range of use is limited. However, the natural composite preservative composition of the present invention appears to be excellent antimicrobial activity in both Gram-positive bacteria, Gram-negative bacteria and fungi, and compared with the average microbial growth minimum inhibitory concentration value for each strain and Test Example 6, mixed in the ratio of Test Example 6 When used to show the microbial growth inhibitory concentration value 70 ~ 80% less than the average value of each was able to confirm the excellent microbial growth inhibitory effect (Table 5 and Figure 1). In other words, when used in combination with the Tahibo extract, Tobokyeong extract, Banga leaf extract and Yangje Geun extract in a constant ratio as in the present invention than when using each extract separately showing a synergistic microbial growth inhibitory effect (synergy effect) It was confirmed.

In order to confirm the toxicity of the natural composite preservative composition of the present invention, the cytotoxicity was confirmed by using the composite natural preservative composition of the combination, when treated with 5% by weight of the composite natural preservative composition, in HaCaT cells and HDF-N cells It was confirmed that the survival rate of 90.1% and 91.2%, respectively, and the antiseptic activity of the natural complex preservative composition is effectively shown in about 1% by weight, it was confirmed that it can be safely used in cosmetic compositions, food and pharmaceutical formulations (Table 6 and Table 7).

In another aspect of the present invention, the present invention relates to a cosmetic containing the above-mentioned natural composite preservative composition as an antiseptic component.

In the present invention, the content of the natural composite preservative composition of the cosmetic may be characterized in that 0.001 to 10% by weight.

In another embodiment of the present invention, a cream formulation was prepared using the natural complex preservative composition mixed in the above various ratios (Table 8), and as a result of confirming the preservative strength of the formulation including the natural complex preservative, Tahibo extract 0.2% by weight, It was confirmed that the formulation with the natural complex preservative composition mixed with 0.05% by weight of Tobokyeong extract, 0.05% by weight of leaf extract and 0.2% by weight of Yangje root extract (Table 9).

In order to confirm the skin irritation degree of the formulation containing the natural complex preservative of the present invention, the skin irritation experiments were conducted in 20 healthy adults and men and women using the formulation including the natural complex preservative in the above combination, and most of them were mild or mild. The average irritation index was lower than that of the cream containing the chemical preservative (Table 10 and Table 11).

In addition, as a result of confirming the stability of the cream formulation prepared using a natural complex preservative composition mixed in various ratios, the phenomenon such as creaming, agglomeration, which is a process of separating the lotion, was not observed. It was confirmed that no odor appeared (Table 12).

In still another aspect of the present invention, the present invention relates to a food containing the above-mentioned natural composite preservative composition as an antiseptic component.

The natural complex preservative composition may be used as a preservative in foods and pharmaceuticals.

The natural complex preservative composition may further contain one or more active ingredients exhibiting the same or similar functions in addition to the Tahibo extract, Tobokyeong extract, Banga leaf extract and Yangje Geun extract.

Cosmetics prepared with a cosmetic composition containing the natural complex preservative composition of the present invention as an active ingredient may be prepared in the form of a general emulsion formulation and solubilized formulation, cosmetics of the emulsion formulations include nutrient cosmetics, creams, essences, etc. Cosmetics of solubilized formulations include soft cosmetics.

Formulation of the cosmetic composition containing the natural complex preservative composition of the present invention as an active ingredient skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisturizing lotion, nutrition lotion, massage cream, nutrition cream, moisture cream, Hand Cream, Essence, Nutrition Essence, Pack, Soap, Shampoo, Cleansing Foam, Cleansing Lotion, Cleansing Cream, Body Lotion, Body Cleanser, Latex, Press Powder, Loose Powder, Eye Shadow, etc. It can be applied to cosmetic preparations. Relief can be applied in various forms such as liquid, cream, paste and solid, and may include various conventional auxiliaries and carriers suitable for each of these formulations and well known in the art.

Hereinafter, the present invention will be described in more detail with reference to Examples. It is to be understood by those skilled in the art that these examples are for illustrative purposes only and that the scope of the present invention is not construed as being limited by these examples.

Example  One. Tahibo  Preparation of extract

The dried tahibo, which had a dry weight of 100 g, was refluxed for 12 hours with 70% (V / V) ethanol aqueous solution, cooled down, and filtered through a filter paper having a permeation size of 1.2 μm. The filtered extract was concentrated under reduced pressure at 50 ° C. or lower and completely dried using a reduced pressure dryer to obtain 6.87 g of Tahibo extract.

Example  2. Tobok Spirit  Preparation of extract

The dried lotus leaf having a dry weight of 100 g was heated to reflux for 12 hours with 70% (V / V) ethanol aqueous solution, and then cooled and then filtered through a filter paper having a permeation size of 1.2 μm. The filtered extract was concentrated under reduced pressure at 50 ° C. or lower, and dried completely using a reduced pressure dryer to obtain 9.65 g of Tobok-ryong extract.

Example  3. Preparation of Membrane Leaf Extract

The dried crushed leaf of 100 g of dry weight was refluxed for 12 hours with an aqueous 70% (V / V) ethanol solution and cooled down, and then filtered through a filter paper having a permeation size of 1.2 μm. The filtered extract was concentrated under reduced pressure at 50 ° C. or lower and dried completely using a reduced pressure dryer to obtain 5.31 g of leaf extract.

Example  4. Yang Jae-geun  Preparation of extract

100 g of dried ginseng root was dried and refluxed for 12 hours with an aqueous 70% (V / V) ethanol solution, and then cooled and filtered through a filter paper having a permeation size of 1.2 μm. The filtered extract was concentrated under reduced pressure at 50 ° C. or lower, and then dried completely using a reduced pressure dryer to obtain 16.5 g of Yangje-Geun extract.

Experimental Example  1. Measurement of the minimum inhibitory concentration of microbial growth

In order to measure the antimicrobial effect of the extracts were investigated by measuring the minimum inhibitory concentration (Minimum Inhibition Concentration, MIC). The strains used to measure the antimicrobial effect were Gram-positive bacteria, Staphylococcusaureus ATCC 6538P, and Gram-negative bacteria, Escherichia. coli ATCC 8739), Pseudomonas aeruginosa ATCC 9027) Candida yeast ( Candyda) albicans ATCC 10231), Aspergillus niger ATCC22343 was used as a filamentous fungus from the Korea Institute of Biotechnology and Biotechnology Center (KCTC, Korea).

Each strain prepared a nutrient broth solution and sterilized at 121 ° C. for 15 minutes, and then inoculated each cultured strain 1 platinum into a nutrient solution (10 ml), and in a shaking incubator. Strains were prepared for the antimicrobial experiment by time incubation.

In order to measure the minimum inhibitory concentration of antibiotics that inhibit the growth of microbial strains, the strains inoculated in sterile medium were inoculated so that the bacteria were 1 × 10 ⁶ cfu / ml and the fungi were 1 × 10 ⁵ cfu / ml. The bacteria were incubated at 35 ° C. for 2 days, and the fungus was incubated at 25 ° C. for 5 days and observed as a control.

On the other hand, each extract is prepared by adding to the medium to 0.01 to 1% by weight, and then sterilized and inoculated to bacteria 1 X 10 ⁶ cfu / ㎖, fungi 1 X 10 ⁵ cfu / ㎖ as in the control group In the case of incubation for 2 days at 35 ℃, for 5 days at 25 ℃ for fungi were observed as an experimental group.

In order to confirm the growth of each strain in the culture solution of the control group and the experimental group, approximately 300 µl of the sample was collected in a sterile space and dispensed into sterile 96-well microplates and absorbance at 650 nm using a spectrophotometer. The microbial proliferation was confirmed by measuring and the minimum concentration showing the low proliferation of each strain was compared with the control group, and the minimum inhibitory concentration (MIC) was determined.

Determination of Minimum Inhibitory Concentration of Microbial Growth of Extracts division Minimum inhibitory concentration (% by weight) S.aureus E. coli P. aeruginosa C. albicans A. niger Tahibo Extract 0.1 0.1 0.5 0.2 0.5 Tobokyeong Extract 0.5 0.1 0.1 0.5 0.5 Membrane Leaf Extract 0.5 0.5 0.5 0.05 0.05 Yangje Root Extract 0.5 0.5 0.2 0.05 0.01

Tahibo extract showed good antimicrobial activity at the concentration of 0.1% by weight against Gram-positive Staphylococcus aureus and Escherichia coli, but relatively weak antimicrobial activity to Gram-positive bacteria Pseudomonas aeruginosa. It showed excellent antimicrobial activity at the concentration of 0.2% by weight, and relatively weak antimicrobial activity against Black Bacillus.

Tobokyeong extract showed good antimicrobial activity against Gram-negative Escherichia coli and Pseudomonas aeruginosa at 0.1% by weight, but showed relatively weak antimicrobial activity against Gram-positive bacteria such as Staphylococcus aureus, Candida yeast and Black Bacillus. .

In the case of M. leaf extract, it showed weak antimicrobial activity against both Gram-negative bacteria and Gram-positive bacteria, but showed effective antimicrobial activity at 0.05 wt% concentration against fungi yeast and filamentous fungi. In the case of Yangje-geun extract, it showed weak antimicrobial activity against bacteria, but it was confirmed that the antimicrobial activity against filamentous fungi was excellent (Table 1).

Example  5. Preparation of Natural Complex Preservatives

In order to confirm the antiseptic effect of the natural composite preservative composition of the present invention, a natural composite preservative composition mixed in various ratios was prepared as in Test Examples 1 to 6 (Table 2).

Natural complex preservative compositions mixed in various proportions number ingredient Test Example 1 Test Example 2 Test Example 3 Test Example 4 Test Example 5 Test Example 6 One Tahibo Extract 0.05 0.05 0.05 0.2 0.2 0.2 2 Tobokyeong Extract 0.05 0.2 0.2 0.05 0.2 0.05 3 Membrane Leaf Extract 0.2 0.05 0.2 0.2 0.05 0.05 4 Yangje Root Extract 0.2 0.2 0.05 0.05 0.05 0.2

Experimental Example  2. Determination of Antiseptic Activity of Natural Complex Preservative Compositions Mixed at Various Ratios

In order to measure the antiseptic activity of the natural composite preservative composition of Test Examples 1 to 6 prepared in Example 5, the experiment was performed so that the final concentration of the natural composite preservative was 0.01%. It used a circular filter paper method (paper disc method) as the test method, using Staphylococcus aureus to Gram-positive bacteria is a strain (Staphylococcusaureus, ATCC 6538P), E. coli gram-negative bacteria (Escherichia coli , ATCC 8739), Pseudomonas aeruginosa , ATCC 9027), candida albicans , ATCC 10231), Aspergillus niger , ATCC22343).

About 100 μl of each of the cultured strains was added to the coagulated plate medium, which was dispensed into plate medium and spread evenly by using a spreader. After absorbing about 60 µl of each extract to be measured on a 10 mm diameter paper disk, the solvent was dried and adhered to the surface of the medium on which the strain was smeared. After incubation, the antimicrobial effect was confirmed by measuring the size of the clear zone formed around the paper disc. As a result, the natural composite preservative showed antiseptic activity in various combinations, it was confirmed that the natural composite preservative composition of Test Example 6 is an excellent combination (Table 3).

Mixed in various proportions Determination of Antiseptic Activity of Natural Composite Preservative Composition Microbial strain S.aureus E. coli P. aeruginosa C. albicans A. niger Average diameter (mm) density(%) 0.1 0.5 0.1 0.5 0.1 0.5 0.1 0.5 0.1 0.5 Test Example 1 12 13 11 13 12 15 12 14 12 16 13 Test Example 2 12 13 11 13 14 15 12 16 08 19 13.3 Test Example 3 11 12 09 10 12 13 11 14 08 12 11.2 Test Example 4 13 15 15 16 12 16 10 12 11 13 13.3 Test Example 5 14 16 15 15 16 15 11 16 12 16 14.6 Test Example 6 16 20 17 19 17 20 18 19 17 20 18.3

Experimental Example  3. Evaluation of Minimum Inhibitory Concentration of Microbial Growth of Natural Complex Preservatives

In order to measure the minimum microbial growth inhibition concentration (MIC) of the natural complex preservatives, the experiment was conducted in the same manner as in Experimental Example 1. As a result, it was confirmed that the overall excellent antimicrobial activity in Test Examples 1 to 6, in the case of Test Example 6 showed excellent antimicrobial activity at a concentration of 0.1% by weight of Gram-positive Staphylococcus aureus, E. coli and Gram-negative bacteria Pseudomonas aeruginosa, Candida and Black Bacillus showed excellent antimicrobial activity at a concentration of 0.05 to 0.08% by weight (Table 4).

In addition, as a result of comparing the average microbial growth inhibitory concentration value and Test Example 6 for each strain, when used in a mixture of Test Example 6 ratio showed a microbial growth minimum inhibitory concentration is reduced 70 ~ 80% than the average value of each Microbial growth inhibitory effect was confirmed (Table 5 and FIG. 1). In other words, when used in combination with the Tahibo extract, Tobokyeong extract, Banga leaf extract and Yangje Geun extract in a constant ratio as in the present invention than when using each extract separately showing a synergistic microbial growth inhibitory effect (synergy effect) It was confirmed.

Evaluation of Minimum Inhibitory Concentration of Microbial Growth for Natural Complex Preservatives division Minimum inhibitory concentration (% by weight) S.aureus E. coli P. aeruginosa C. albicans A. niger Test Example 1 0.5 0.5 0.2 0.2 0.5 Test Example 2 0.2 0.2 0.5 0.5 0.2 Test Example 3 0.5 0.5 1.0 1.0 0.5 Test Example 4 0.2 0.2 0.5 0.5 0.5 Test Example 5 0.2 0.2 0.1 0.1 0.01 Test Example 6 0.1 0.08 0.08 0.05 0.05

Comparative evaluation of microbial growth minimum inhibitory concentration of each extract and Test Example 6 division Minimum inhibitory concentration (% by weight) S.aureus E. coli P. aeruginosa C. albicans A. niger Extract average 0.4 0.3 0.325 0.2 0.265 Test Example 6 0.1 0.08 0.08 0.05 0.05

Experimental Example  5. Cytotoxicity Assessment of Natural Complex Preservatives

In order to confirm the toxicity of the natural complex preservatives, cytotoxicity was measured in HaCaT cells and HDF-N cells using Test Example 6 having the highest antiseptic activity in the above experimental examples. HaCaT cells were dispensed at a concentration of 5 × 10 ⁴ cells per well in a 96 well plate, and HDF-N cells were dispensed at a concentration of 6 × 10 ³ cells per well in a 96 well plate and incubated in cell culture conditions for 24 hours. After the medium was removed and washed with DPBS, starvation was maintained for 12 hours, sample solution and fresh medium (except for media supplement) were added, and cultured for 24 hours. After removing the sample solution and the medium and washing with PBS, in order to measure the survival rate of the cells, the WST-1 reaction solution was diluted 1/10 in the medium without supplement and treated with 100 μl of each well for 1 hour. After the absorbance was measured at 450 nm.

As a result, it was confirmed that the survival rate of 90.1% and 91.2% in HaCaT cells and HDF-N cells, respectively, and the antiseptic activity of the natural complex preservative composition is effectively shown in about 1%, so it is safe for cosmetic compositions, food and pharmaceutical formulations. It was confirmed that it could be used (Table 6 and Table 7).

Cytotoxicity Assessment of HaCaT Cells from Natural Complex Preservatives sample Control group Experimental group [Cytotoxicity against HaCaT cell] density 0 0.01 0.1 0.5 1.0 2.0 5.0 %
Control 100.0 101.5 100.1 99.5 98.1 94.0 90.1

Cytotoxicity Evaluation of Natural Complex Preservative Compositions on HDF-N Cells sample Control group Experimental group [cytotoxicity against HDF-N cell] density 0 0.01 0.1 0.5 1.0 2.0 5.0 %
Control 100.0 102.1 99.0 97.9 95.2 93.4 91.2

Example  6. Preparation of Cream Formulations Containing Natural Complex Preservatives Mixed in Various Ratios

In order to perform an antiseptic test of a cosmetic containing a natural composite preservative composition of the present invention, a cream formulation containing a natural complex preservative composition mixed in various ratios was prepared as Comparative Examples 1 and 1 to 6 (Table 8). ).

Preparation of cream formulations containing natural complex preservatives mixed in various proportions ingredient Comparative Example 1 Test Example 1 Test Example 2 Test Example 3 Test Example 4 Test Example 5 Test Example 6 Purified water Balance Balance Balance Balance Balance Balance Balance glycerin 5.0 5.0 5.0 5.0 5.0 5.0 5.0 Butylene glycol 7.0 7.0 7.0 7.0 7.0 7.0 7.0 Carbomer 0.2 0.2 0.2 0.2 0.2 0.2 0.2 Xanthan gum 0.08 0.08 0.08 0.08 0.08 0.08 0.08 Cetearyl alcohol 3.0 3.0 3.0 3.0 3.0 3.0 3.0 Glyceryl stearate 0.5 0.5 0.5 0.5 0.5 0.5 0.5 Behenyl alcohol 2.0 2.0 2.0 2.0 2.0 2.0 2.0 Hydrogenated lecithin 0.2 0.2 0.2 0.2 0.2 0.2 0.2 Propylparaben 0.1 0.1 0.1 0.1 0.1 0.1 0.1 Mineral oil 3.0 3.0 3.0 3.0 3.0 3.0 3.0 Vegetable squalane 3.0 3.0 3.0 3.0 3.0 3.0 3.0 Cetyl hexanoate 3.0 3.0 3.0 3.0 3.0 3.0 3.0 Arginine 0.2 0.2 0.2 0.2 0.2 0.2 0.2 Tahibo Extract - 0.05 0.05 0.05 0.2 0.2 0.2 Tobokyeong Extract - 0.05 0.2 0.2 0.05 0.2 0.05 Membrane Leaf Extract - 0.2 0.05 0.2 0.2 0.05 0.05 Yangje Root Extract - 0.2 0.2 0.5 0.05 0.05 0.2 Methyl paraben 0.2 - - - - - -

Experimental Example  5. Confirmation of Preservative Effect of Formulations Containing Natural Complex Preservatives

The antiseptic effect on Comparative Example 1 and Test Examples 1 to 6 was performed using the test method of microbiology guidelines of The Cosmetic, Toility and Fragrance Association, Inc. (hereinafter referred to as CTFA). Staphylococcus aureus with a gram-positive bacteria is a strain (Staphylococcusaureus, ATCC 6538P), E. coli gram-negative bacteria (Escherichia coli , ATCC 8739), Pseudomonas aeruginosa , ATCC 9027), candida albicans , ATCC 10231), Aspergillus niger , ATCC22343). In a bottle containing 10 g of Comparative Examples and Test Examples , a suspension of three bacteria ( E. coli , P. aeruginosa , S. aureus ) and two fungi ( C. albicans, A. niger ) was mixed. Inoculate ⁶ cfu / g, 1 × 10 ⁵ cfu / g, and store 1 g of the sample under aseptic operation at 4 hours, 1 day, 2 days, 3 days, and 7 days after storage at 25 ° C. Properly diluted, bacteria were cultured in Nutrient agar (Difco) and fungi in Potato dextrose agar (Difoc) medium and counted after incubation at 35 ° C and 25 ° C for 24 to 72 hours, respectively. As a result, since the antiseptic effect of the cream containing the composite natural preservative of Test Example 6 shows an effect similar to that of the comparative example used as a conventional synthetic preservative (Table 9), the natural composite preservative composition of the present invention is very excellent antiseptic effect. It was confirmed to have.

Confirmation of Preservative Effect of Formulations Containing Natural Complex Preservatives Fungus D-value (hr root) Comparative Example 1 Bacterial death 4.2 Fungal death 6.5 Test Example 1 Bacterial death 6.2 Fungal death 7.5 Test Example 2 Bacterial death 5.8 Fungal death 7.8 Test Example 3 Bacterial death 6.2 Fungal death 8.9 Test Example 4 Bacterial death 4.6 Fungal death 7.5 Test Example 5 Bacterial death 4.9 Fungal death 6.9 Test Example 6 Bacterial death 4.1 Fungal death 4.2

Experimental Example  6. Skin irritation test of natural complex preservative

The skin irritation test was carried out with the natural preservative composition of Test Example 6 of the cream formulation carried out in Example 6 for the skin irritation test of the natural complex preservative.

Twenty healthy men and women were tested according to CTFA guidelines (The Cosmetic, Toility and Fragrance Association, Inc. Washington DC, 20036, 1991). 15 mg / 15 μl of the sample and the solution were added dropwise to a pin chamber, and it was placed on the arm of the human body as a test site and fixed with tape. Subsequently, after 24 hours of patching, the patch was removed and after 2 hours, the skin reaction at the test site was judged according to the following criteria, which was mostly irritating or mild and averaged over creams containing chemical preservatives. It was confirmed that the stimulation index is low (Table 10 and Table 11).

Criteria based on skin results division score Criteria No irritation 0 No reaction Slight irritation 0.5 Faint or light erythema One Clear or weak erythema Strong stimulation 2 Distinct erythema with papules and edema 3 Severe erythema and blister formation

Skin irritation test of natural complex preservative division Causes of irritation Average Stimulus Index No irritation Slight irritation Strong stimulation Test Example 6
(0.5% Preservative Prescription Cream) 19 One 0 0.025 1% Preservative Cream 18 2 0 0.05 Control group
(Mbangbang cream prescription) 20 0 0 0

Experimental Example  7. Stability Test of Formulation

The creams of Test Examples 1-6 of Example 6 containing the natural complex preservative composition and the cream (control) not containing the natural complex preservative composition were changed at different temperatures (0, 25, 37, 45, daylight) for 12 weeks. After storage, visual observation of the cream and changes in smell were observed. As a result, both the control and the cream containing Test Examples 1 to 6 were not observed such as creaming and agglomeration, which are processes of separating the lotion for 12 weeks, and there was no specific odor due to oxidation of the lotion component ( Table 12).

Experimental Stability of Formulations According to Temperature Conditions division Formulation Stability with Temperature Conditions 0 ℃ 25 ℃ 37 ℃ 45 ° C daylight Comparative Example 1 0 0 0 0 0 Example 5 0 0 0 0 0 Example 6 0 0 0 0 0 Example 7 0 0 0 0 0 Example 8 0 0 0 0 0 Example 9 0 0 0 0 0 Example 10 0 0 0 0 0

0: no change, 2: slight change, 4: severe change (changing, discoloration, separation)

As described above, specific parts of the present disclosure have been described in detail, and for those skilled in the art, these specific descriptions are merely preferred embodiments, and the scope of the present disclosure is not limited thereto. Will be obvious. It is therefore intended that the scope of the invention be defined by the claims appended hereto and their equivalents.

Claims (5)

Natural complex preservative composition containing Tahibo extract, Tobokyeong extract, Banga leaf extract and Yangje Geun extract.
The natural composite preservative composition according to claim 1, wherein the tahibo extract, the Tobok-ryong extract, the leaf extract, and the extract of Yangje-Geun are 0.01 to 2.0% by weight, respectively.
Cosmetics containing the natural composite preservative composition of claim 1 or 2 as an antiseptic component.
The cosmetic according to claim 3, wherein the content of the natural composite preservative composition is 0.001 to 10% by weight.
Food containing the natural complex antiseptic composition of Claim 1 or 2 as an antiseptic component.

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