KR101694229B1 - Cosmetic composition comprising sacha inchi oil and extract of Portulaca oleracea L. - Google Patents

Abstract

The present invention relates to a cosmetic composition containing a saccharin oil and a horseradish extract. More specifically, the present invention relates to a cosmetic composition comprising saccharin oil and a marigold extract to prevent skin aging, improve wrinkles, whitening And to a cosmetic composition capable of exhibiting an acne skin improving effect.
The cosmetic composition of the present invention can exhibit effects of preventing skin aging, improving wrinkles, whitening, and improving acne skin, and by using natural raw materials, it can be environmentally friendly, and skin troubles can be sufficiently satisfied. In addition, it is expected to be competitive in cosmetics market in Vietnam and other countries because it can show excellent effect at low price compared to other functional cosmetics.

Description

[0002] Cosmetic composition comprising sachain oil and extract of Portulaca oleracea L.,

The present invention relates to a cosmetic composition containing a saccharin oil and a horseradish extract. More specifically, the present invention relates to a cosmetic composition comprising saccharin oil and a marigold extract to prevent skin aging, improve wrinkles, whitening And to a cosmetic composition capable of exhibiting an acne skin improving effect.

For modern people who are making a lot of efforts to improve their appearance, skin aging, wrinkles, dark skin and acne are always a big headache. In order to solve these problems, various studies such as medical care and cosmetics are being carried out, and various kinds of pharmaceuticals and cosmetics related thereto are being developed.

In Vietnam, the demand for acne care cosmetics has increased recently due to the high temperature and humidity of the climate. About 40% of the population has oily skin type, and the problem of acne caused by excessive oil, sebum, high. Therefore, in order to prevent and effectively manage the occurrence of acne, various acne solution cosmetics tend to favor.

Meanwhile, in recent years, consumer preferences for environmentally friendly products are increasing due to various environmental problems and side effects caused by chemical synthetic substances. Especially, in the field of cosmetics directly contacting with the human body, such tendency becomes more clear. In this regard, Korean Patent No. 10-1413328, No. 10-1402550, etc., Have been developed. Recently, the most prominent feature in the Vietnamese cosmetics market is that consumers are more likely to favor natural products. In fact, pure vegetable low-cost cosmetics launched in line with recent trends to consume natural products are popular.

The present inventors have developed a cosmetic product which is excellent in the effect of preventing skin aging, improving wrinkles, whitening and acne skin, and being environment friendly and having less skin troubles by using natural raw materials, In particular, we have conducted various studies to develop competitive products in the cosmetics market in Vietnam and in other cosmetics markets in the world.

Korean Patent No. 10-1413328 Korean Patent No. 10-1402550

Accordingly, it is a main object of the present invention to provide a cosmetic composition which is excellent in the effect of preventing skin aging, improving wrinkles, whitening and acne skin, and containing natural raw materials, thereby being environmentally friendly and having less skin troubles.

According to one aspect of the present invention, there is provided a cosmetic composition for skin aging prevention, wrinkle improvement, whitening, and acne skin improvement comprising sacha inchi (Plukenetia volubilis) oil and Portulaca oleracea L. extract do.

In the cosmetic composition of the present invention, it is preferable that the above-mentioned saccharide oil and mash extract are contained in an amount of 0.1 to 2% by weight based on the total weight of the composition. According to the present invention, it is possible to effectively exhibit antibacterial, antioxidative and whitening effects of Sakka-in-Oki oil and mashish extract at the above concentrations, and it is preferable in view of skin irritation and feeling of use of the manufactured cosmetics.

In the cosmetic composition of the present invention, the above-mentioned saccharin-derived oil can be obtained by either commercially available oil or by directly preparing an oil extracted from the saccharin-yaki fruit according to a conventional method for extracting oil from a plant.

In the cosmetic composition of the present invention, it is preferable that the marigold extract is a liquid extract obtained by adding water and brown sugar to a dried marigold and aging or fermenting it. According to this, more excellent antibacterial activity can be exhibited. More preferably, 300 to 1000 parts by weight of water and 300 to 1000 parts by weight of brown sugar are added to 100 parts by weight of the marigold extract, followed by aging at 20 to 30 DEG C for 15 days to 3 months, or by fermenting lactic acid bacteria to obtain a liquid phase It is good to use it.

The cosmetic composition of the present invention can be formulated into various forms according to the use, and in particular, when it is formulated with a water cream or mask pack, it is effective for preventing skin aging, improving wrinkles, whitening and improving acne skin.

The cosmetic composition of the present invention can exhibit effects of preventing skin aging, improving wrinkles, whitening, and improving acne skin, and by using natural raw materials, it can be environmentally friendly, and skin troubles can be sufficiently satisfied. In addition, it is expected to be competitive in cosmetics market in Vietnam and other countries because it can show excellent effect at low price compared to other functional cosmetics.

Fig. 1 shows the aging or fermentation process for obtaining dried march and march extract.
Fig. 2 shows a process for measuring antimicrobial activity of Sakaeginky oil and mashii extract.
Fig. 3 shows the result of measurement of antimicrobial activity of Sakae Ink Oil.
Fig. 4 shows the results of measurement of antimicrobial activity of the extract of Ganoderma lucidum. Left upper side of each plate: A sample of Example 1, upper right: B sample, lower left: C sample, lower right: D sample.
Fig. 5 shows the analysis of antioxidant activity of Sakaeginky oil.
Figs. 6 and 7 show the results of the antioxidative activity analysis of Sakae Ink Oil.
Fig. 8 shows a process for analyzing the whitening activity of Sakae Ink Oil.
Fig. 9 shows the results of the whitening activity analysis of Sakae Ink Oil.
10 and 11 illustrate a process for producing a water cream of the present invention.
12 shows a process of testing the formulation and application feeling of the water cream of the present invention.
13 and 14 show a mask pack manufacturing process of the embodiment of the present invention.
15 shows a questionnaire for skin irritation safety evaluation used in the examples of the present invention.
16 shows a questionnaire for use feeling evaluation used in the embodiment of the present invention.
Figs. 17 and 18 show the result of feeling feeling evaluation when the moisture cream of the embodiment of the present invention is applied for the first time.
FIGS. 19 and 20 show skin condition evaluation results after applying the water-based cream of the embodiment of the present invention.

Hereinafter, the present invention will be described in more detail with reference to Examples. These embodiments are only for illustrating the present invention, and thus the scope of the present invention is not construed as being limited by these embodiments.

EXAMPLES Example 1 Preparation of Sakka Glycyrrhiza oil and Ganoderma lucidum extract

1-1. Sakae oil

Sakaegy oil (commercially available from Sakka Ink) was purchased and used in this example.

1-2. Marchia extract

Dried marigolds were blended in the following proportions respectively.

A: 100 g of dried horseradish, 500 ml of water, 500 g of white sugar

B: 100 g of dried germ, 500 ml of water, 500 g of brown sugar

C: 100 g of dried gruel, 500 ml of water, 500 g of white sugar

D: 100 g of dried horseradish, 500 ml of water, 500 g of brown sugar

Samples A and B were naturally fermented, and C and D were fermented by inoculating 50 ml of EM (useful microorganism) each as a fermentation starter. The aging or fermentation temperature was 25 ° C for one month. Liquid extracts produced after fermentation or fermentation were used in this example (see Figure 1).

Example 2: Antimicrobial activity measurement of saccaintle oil and horseradish extract

Staphylococcus aureus (KCTC3881), an aerobic Gram (+) strain, and Escherichia The coli (KCTC1039) and Pseudomonas aeruginosa (KCTC1637), Candia albicans (KCTC7965), Aspergillus niger , also known as black aspergillosis (KCTC6910) by fungi that cause candidiasis, and take pre-sale use them in a biological resource center (KCTC).

To test the antimicrobial activity, each strain stored at -80 ° C was taken out to dissolve, and S. aureus and P. aeruginosa were cultured in TSA (trypic soy agar) medium, E. coli were cultured in LB (Luria-Bertani media) medium, C. albicans Were inoculated in YEPD medium and A. niger in a PDA (Potato Dextorse agar) medium, respectively, and cultured at 30 ° C. for 18 to 48 hours.

Strain Strain number badge Escherichia coli KCTC 1039 LB Staphylococcus aureus KCTC 3881 TSA Pseudomonas aeruginosa KCTC 1637 TSA Candida albicans KCTC 7965 YEPD Aspergillus niger KCTC 6910 PDA

The paper-disc method was used to measure the antimicrobial activity of saccaine oil and mash extract. A single colony of each cultivated strain was inoculated on a solid medium and then cultured at 30 ° C. for 16 hours or longer. The sterilized paper-disc was placed on a well-smoothed plate so that the flat portion of the paper- Next, 50 μl of Sakae oil or extract is taken and the paper-disc is wetted. The strain was cultured under the condition of each strain, and a clear zone was confirmed after culturing (see FIG. 2).

As a result, as shown in FIG. 3, SAKA YUKI OIL showed no antimicrobial activity against the test strain, as expected, whereas the MAKINA extract showed antimicrobial activity as shown in FIG. Therefore, it is concluded that the extracts of Ganoderma lucidum can sufficiently complement the deficient antimicrobial activity of Sakae oil.

Example  3. Sakae Ginky  Exploring the antioxidant activity of oils

The antioxidant activity of saccaine oil was investigated as a primary criterion for predicting whether it would be useful as a functional cosmetic material by measuring its ability to eliminate free radicals causing skin aging. Antioxidant activity was assessed as scavenging capacity to capture the free radicals of DPPH (2,2-diphenyl-1-picrylhydrazyl). DPPH itself is a very stable free radical substance, which is a violet compound exhibiting absorbance at 517 nm and is discolored from purple to yellow quantitatively by radical scavenging antioxidants, It is widely used in search.

0.5 ml of a sample (0.5 ml / 10 ml) dissolved in ethanol was added to 1 ml of a 0.2 mM concentration of ethanol solution of DPPH, and the mixture was allowed to stand at 24 ° C for 30 minutes. The absorbance at 517 nm was measured to determine the concentration of DPPH (See Fig. 5). The free radical scavenging activity was calculated by the following equation. As a positive control sample, vitamin C (ascorbic acid) prepared at 10 mg / ml was used.

% scavenging = [1- (B-C) / A] x 100 (%)

A: absorbance after reaction without addition of sample

B: Absorbance after reaction with addition of sample

C: absorbance measured by replacing with DPPH by distilled water

As a result, as shown in Fig. 6 and Fig. 7, Sakaegy oil showed better antioxidative activity than the positive control vitamin C. Therefore, it is considered that the addition of Sakae Ink Oil to cosmetics will be effective in preventing skin aging and preventing wrinkles.

Example 4. Detection of whitening activity of Sakka-Yaki oil

Currently, functional whitening activity is recognized as functional cosmetics in Korea, and whitening active functional cosmetics are actively developed. In addition, several test methods for assessing whitening activity have been proposed by the Korea Food and Drug Administration, one of the easiest ways to do this is to evaluate the inhibition of DOPA oxidation by tyrosinase (tyrosinase in vitro DOPA oxidative activity inhibition test). Thus, in this Example, the whitening activity was evaluated by this method. In this method, the tyrosinase inhibitory activity is measured by measuring the amount of DOPA-chrome produced from DOPA by the action of tyrosinase. Within the cell, DOPA-chrome is ultimately converted to melanin by the enzyme. Therefore, this method is effective as a measurement method of whitening activity.

To each well of a 96 well plate, add 850 μL of 0.1 M phosphate buffer (pH 7.0), 50 μL of sample solution (10 mg / mL), 50 μL of mushroom tyrosinase (2000 U / mL) and 50 μL of 6 mM DOPA as a substrate solution For 1 minute, and the absorbance at 475 nm was measured using a microplate reader (see FIG. 8). As a positive control sample, albutin and vitamin C, which were already commercially available, were used because of their already whitening activity.

Tyrosinase inhibitory activity was calculated as follows.

Inhibition rate (%) = [1- (C-D / A-B)] 100 (%)

A: The absorbance (tyrosinase + phosphate + DOPA)

B: The absorbance (tyrosinase + phosphate) of the reaction solution containing only tyrosinase and buffer solution

C: The absorbance of the reaction solution containing the sample (tyrosinase + phosphate + sample + DOPA)

D: Absorbance (tyrosinase + phosphate + sample) of the reaction solution without addition of DOPA

As a result, nearly 100% tyrosinase inhibition was observed in the case of vitamin C used as a positive control as shown in Fig. 9 in the test concentration (10 mg / ml, when the same 50 μl of the sample was added to the reaction liquid = the solid concentration of the sample) Lt; / RTI > On the other hand, arbutin, a commercially available whitening ingredient, showed about 83.4% inhibitory activity at the same concentration. Sakaegy oil showed 156.8% inhibitory activity, which is about 56.8% higher than vitamin C and 73.4% higher than arbutin. As a result, it can be developed as a cosmetic material in addition to the excellent antioxidant effect of Sakae Ink Oil.

Example 5. Development of water cream formulation

In order to develop a stable formulation, a water cream was prepared as follows (see FIGS. 10 and 11), in which the content of Sakka-in-Oki oil and mash extract was varied from 0.1 to 2%.

cream Ingredients (Ingredients) 100


Paid One TIO 3 2 Dermafeel BGC 3 3 Squalane 4 4 Beeswax One 5 Arlacel 165 0.5 6 Cetanol 1.5 7 DM / DP 0.1 / 0.05 8 Montanov-L 3

Awards 9 D.I.Water 38.55 10 Butylene glycol (1,3) 4 11 Glycerine 6 12 hyaluronic acid (1%) 3 13 Allantoin 0.1 14 Arbutin One additive 15 Sakae oil 0.1 to 2% 16 Marchia extract 0.1 to 2% 17 DC245 5 18 T.E.A (10%) 4 19 Cabopol Ultrez-21 (2%) 20 20 Perfume 0.1

Unit: wt%

- 1 to 8 are completely dissolved at 75 ℃ as an oil phase.

- Add 9 to 14 times to the No. 9 purified water, add 10 to 14 times, heat to 75 ℃ and completely dissolve.

- While the homomixer is kept at a speed of 5,000 rpm in the water phase, the oil phase is added and emulsified.

- After dropping the temperature to 65 ° C, add 17 to 19 times.

- Adjust to 2000 rpm at 50 ℃ and add 15, 16 and 20 to complete the emulsification.

- Cool to 30 ℃, defoam, filter and store to complete the cream.

Example 6. Development of mask pack formulation

Sakaegki oil and mash extract were prepared under the condition of 0.1 ~ 2%. After the first essence was prepared, the mask sheet was immersed in a beaker in which the essence was prepared and stored in a zipper bag. The manufacturing method was as follows (see Figs. 13 and 14) .

essence Ingredients (Ingredients) 300g Paid DC245 18 Caprylic triglyceride 4.5


Awards D.I.Water To 300 Butylene glycol (1,3) 18 Glycerine 9 Nikkol HCO-60 1.8 T.E.A (10%) 12 Cabopol Ultrez-21 1.2 hyaluronic acid (1%) 3 Other Sakae oil extract / Perilla extract Q.S
Ethanol phase Ethanol (95%) 6 Methyl Paraben 0.6 Propyl Paraben 0.15

Unit: g

- The water phase and oil phase are each stirred in a stirred thermometer and kept at 75 ° C.

- Add the oil part to the water-receiving part and stir with a homomixer at 5000 rpm for 3 minutes.

- Add DC-245, Carbopol, and TEA in this order at 65 ° C and stir at 5000 rpm for 2 minutes with a homomixer.

- Transfer to a 50 ° C stirrer and cool to 45 ° C with slow stirring.

- Add flavor, coloring matter, and functional extract. After stirring for 2 minutes, cool.

Example 7. Assessment of skin irritation safety of water cream

The optimum mixing ratio of water cream formulations was established by varying the mixing ratio of SAKA INDIAN OIL and MAKIHI EXTRACT. In order to confirm whether the developed product is safe when applied to human body, it is divided into subjective stimulation and objective stimulation The questionnaire (see Fig. 15) was used to evaluate the degree of the stimulus. The subjects were selected from 11 female subjects aged 20 ~ 24 years who were adhered to the purpose of the test through the skin condition diagnosis for university students who usually use basic cosmetics.

The results are shown in Table 4.

Moisture Cream Number of respondents (%) Skin irritation Very strong Strong weakness none



Subjective
Stimulation Stiffening 0 One 2 8 Itching (pruritus) 0 0 0 11 (100) Stinginess 0 2 2 7 Burning (burning sensation) 0 One One 9 Bitter 0 One One 9 ache 0 0 2 9 Pulling / Fastening 0 0 4 7 Snow forest 0 0 One 10 Snowstorm 0 0 0 11 Eye pain (pain) 0 0 0 11 Other( ) 0 0 0 11




Objective
Stimulation Redness (erythema) 0 0 0 11 Swelling (swelling) 0 0 0 11 Milk sprouts 0 0 0 11 Hardening (hardening) 0 0 One 10 pimple 0 0 One 10 acne 0 0 One 10 Keratinized 0 0 One 10 Blister or blister 0 0 0 11 Pigmentation 0 0 0 11 Eye congestion 0 0 0 11 Tear 0 0 0 11 Other( ) 0 0 0 11

Example 8. Evaluation of feeling of use of moisture cream

In order to evaluate the sensory evaluation of the product, 30 female subjects aged 20 to 50 years who were connected to the test purpose by skin condition diagnosis were selected and evaluated for college students, workers and housewives who usually use basic cosmetics. The method was performed by applying a moisturizing cream to the normal condition and evaluating the subjective feeling of the skin condition after 10 to 30 minutes after the first application (see FIG. 16).

The results are shown in Figs. 17 to 20.

Claims (4)

A cosmetic composition for improving skin wrinkles, whitening and acne skin, wherein the extract is a liquid extract obtained by adding water and brown sugar to a dried marinade, and aging or fermenting the extract. The method according to claim 1,
The cosmetic composition for improving skin wrinkles, whitening and acne skin, wherein the sarka ginky oil and mash extract are contained in an amount of 0.1 to 2% by weight based on the total weight of the composition. delete The method according to claim 1,
The cosmetic composition for improving skin wrinkles, whitening, and acne skin, wherein the cosmetic composition is a formulation of a water cream or a mask pack.

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