KR102176618B1 - Composition comprising extract of zea mays linne - Google Patents

Abstract

The present invention relates to a composition containing an extract of Okchok calligraphy as an active ingredient, wherein the extract of Okchok calligraphy significantly inhibits the formation of melanin and the expression of IL-6, and a cosmetic composition for improving or whitening skin troubles, inflammation or pain It may be usefully used as a cosmetic composition for preventing or improving of, a pharmaceutical composition for preventing or treating inflammation or pain, a health food composition for preventing or improving inflammation or pain.

Description

Composition comprising extract of zea mays linne as an active ingredient

The present invention relates to a composition containing an extract of Okchok Calligraphy as an active ingredient, and more specifically, a cosmetic composition for improving or whitening skin troubles containing a flower stalk and pistil head extract of rice family corn (zea mays linne) as active ingredients It relates to a cosmetic composition for preventing or improving inflammation or pain, a pharmaceutical composition for preventing or treating inflammation or pain, and a health food composition for preventing or improving inflammation or pain.

The skin is a very important tissue that protects the human body while directly in contact with the external environment and performs biochemical and physical functions. Skin can be divided into dry, normal and oily skin, which is not maintained in a fixed state for a lifetime, but can change according to season, age, dietary habits and environmental factors. That is, even if the skin condition is neutral, it may change into sensitive skin depending on dietary habits and environmental factors, or to complex skin that appears in combination with dry and oily skin, causing skin trouble. In particular, there is a high concern of skin damage due to the recent problems of air pollution and exposure to ultraviolet rays due to the destruction of the ozone layer.

Symptoms of such skin troubles may appear in various forms, such as allergic and atopic skin accompanied by itching and inflammation, and acne skin caused by bacterial and hormonal changes. In the case of skin problems that react sensitively to the external environment, such as allergic and atopic skin, in the past, it mainly occurred in younger generations under the age of 10, but now it is widely occurring throughout all generations.

Meanwhile, preference for white skin is increasing due to skin damage and pigmentation caused by ultraviolet rays, inflammation and hormones, as well as skin troubles, and interest in skin whitening is increasing as the pursuit of clearer and clearer skin. Pigmentation occurs in melanocytes in the basal layer of the epidermis, and is known to be most affected by control of tyrosinase expression until now. Melanin is a biopolymer material of phenols that is widely distributed in nature. It is a complex of black pigment and protein that enhances the viability and competitiveness of cells against the environment. Melanin exists in the skin and performs an important function to protect the body from ultraviolet rays, but it has been reported that excessive production of melanin forms spots and freckles in the human body, promotes skin aging, or causes skin cancer. In addition, excessive synthesis of melanin, which occurs as exposure to ultraviolet rays becomes serious due to the destruction of the ozone layer, causes fatal harm to skin aging of the human body.

Accordingly, researches on developing cosmetics that have no side effects, are human-friendly, and can be safely used for specific constitutions, atopic skin, or sensitive skin are being actively conducted. Plant extracts are not only excellent in safety, but also contain ingredients that are beneficial to the human body, so they can provide effects such as trouble improvement and whitening. Accordingly, there has been an effort to find an extract that is excellent in safety and contains functional substances by selecting plants having a skin trouble improvement and whitening function.

On the other hand, Okchok Calligraphy refers to fresh flower stalks and pistil heads of rice and maize (zea mays linne) in Korea. Okchok calligraphy has a characteristic odor and sweet and light taste, and is known to be effective in swelling caused by pyelonephritis, jaundice hepatitis, cholecystitis, gallbladder stones, high blood pressure, etc. However, there has been no research on skin trouble improvement or whitening effect using Okchok Calligraphy.

Korean Patent Registration No. 10-1888133 (Registered on Aug. 7, 2018)

An object of the present invention is to provide a cosmetic composition for improving or whitening skin problems.

Another object of the present invention is to provide a cosmetic composition for preventing or improving inflammation or pain, a pharmaceutical composition for preventing or treating inflammation or pain, and a health food composition for preventing or improving inflammation or pain.

In order to achieve the above object, the present invention provides a cosmetic composition for improving or whitening skin troubles containing an extract of Okchok calligraphy as an active ingredient.

In addition, the present invention provides a cosmetic composition for preventing or ameliorating inflammation or pain, a pharmaceutical composition for preventing or treating inflammation or pain, and a health food composition for preventing or improving inflammation or pain, containing the extract of Okchok calligraphy as an active ingredient. .

The extract of the present invention remarkably inhibits the formation of melanin and the expression of IL-6, a cosmetic composition for improving or whitening skin problems, a cosmetic composition for preventing or improving inflammation or pain, preventing or treating inflammation or pain It can be usefully used as a pharmaceutical composition for, a health food composition for preventing or improving inflammation or pain.

1 is a photograph showing an example of Okcheok calligraphy.
2 shows the effect of inhibiting melanin formation by the extract of Okchokseoye using mouse melanocytes (B16F10).
3 shows the effect of inhibiting IL-6 expression by the extract of Okchokseoye using mouse macrophages (RAW264.7).
4 is a diagram showing a manufacturing process chart of a cosmetic containing an extract of Okchok calligraphy.

The inventors of the present invention confirmed that the extract of jade chok calligraphy, in detail, the flower stalk and pistil head extract of the rice family corn (zea mays linne) inhibits melanin formation and the expression of IL-6 to improve skin troubles and whitening effect The invention was completed.

Thus, the present invention provides a cosmetic composition for improving or whitening skin troubles containing an extract of Okchok calligraphy as an active ingredient.

The okchok calligraphy refers to the flower stalk and pistil head of the rice family corn (zea mays linne).

It should be noted that the extract of Okchok calligraphy may be extracted by hot water extraction, but is not limited thereto.

It should be noted that the extract may be included in an amount of 4 to 10 parts by weight based on 100 parts by weight of the total composition, but is not limited thereto.

The extract of Okchokseoye can inhibit melanin formation and IL-6 expression.

The cosmetic composition may be formulated in a formulation selected from the group consisting of lotion, essence, lotion, cream, cleansing foam, powder, pack, gel, ointment, patch, stick, emulsion, and spray, but is not limited thereto. do.

When the composition of the present invention is a cosmetic composition, the cosmetic composition may include conventional auxiliary agents such as stabilizers, solubilizers, vitamins, pigments and fragrances, and a carrier in addition to the active ingredients. In addition, the cosmetic composition may further include a skin absorption accelerator to enhance its effect.

The formulation of the cosmetic composition may be prepared in any formulation conventionally prepared in the art, for example, the cosmetic composition is a lotion, emulsion, skin, toner, lotion, essence, sunscreen, cream, makeup base, foundation , Powder, pack, gel, ointment, patch, stick, shampoo, rinse, spray, makeup remover and detergent, etc. may be formulated, but is not limited thereto.

When the formulation is a paste, cream or gel, animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivatives, polyethylene glycol, silicone, bentonite, silica, talc, or zinc oxide may be used as a carrier component. .

When the formulation is a powder or spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component, and in particular, in the case of a spray, additional chlorofluorohydrocarbon, propane/butane Or a propellant such as dimethyl ether.

When the formulation is a solution or emulsion, a solvent, a solubilizing agent or an emulsifying agent is used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1,3 -Butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or fatty acid ester of sorbitan.

When the above formulation is a suspension, a liquid diluent such as water, ethanol or propylene glycol as a carrier component, an ethoxylated isostearyl alcohol, a suspending agent such as polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, microcrystalline cellulose , Aluminum metahydroxide, bentonite, agar or tracant, and the like may be used.

In addition, the present invention provides a cosmetic composition for preventing or improving inflammation or pain, containing the extract of Okchok calligraphy as an active ingredient.

In addition, the present invention provides a pharmaceutical composition for the prevention or treatment of inflammation or pain containing the extract of Okchokseoye as an active ingredient.

When the composition of the present invention is a pharmaceutical composition, for administration, it may include a pharmaceutically acceptable carrier, excipient, or diluent in addition to the above-described active ingredients. Examples of the carrier, excipient and diluent include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, gum acacia, alginate, gelatin, calcium phosphate, calcium silicate, cellulose, methyl cellulose, microcrystalline cellulose, Polyvinylpyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate, and mineral oils.

The pharmaceutical compositions of the present invention can be formulated and used in the form of oral dosage forms such as powders, granules, tablets, capsules, suspensions, emulsions, syrups, aerosols, etc., external preparations, suppositories, or sterile injectable solutions according to a conventional method. . Specifically, when formulated, it may be prepared using diluents or excipients such as fillers, weight agents, binders, wetting agents, disintegrants, and surfactants that are commonly used. Solid preparations for oral administration include, but are not limited to, tablets, pills, powders, granules, capsules, and the like. Such a solid preparation may be prepared by mixing at least one excipient, for example, starch, calcium carbonate, sucrose, lactose, gelatin, etc. in addition to the active ingredient. Further, in addition to simple excipients, lubricants such as magnesium stearate and talc may also be used. It can be prepared by adding various excipients, such as wetting agents, sweetening agents, fragrances, preservatives, and the like, in addition to oral liquids and liquid paraffin. Formulations for parenteral administration include sterile aqueous solutions, non-aqueous solvents, suspensions, emulsions, lyophilized formulations, and tasks. As the non-aqueous solvent and suspending agent, propylene glycol, polyethylene glycol, vegetable oils such as olive oil, injectable esters such as ethyl oleate, and the like may be used. As a base for suppositories, witepsol, macrosol, Tween 61, cacao butter, laurin, glycerogelatin, and the like may be used.

A suitable dosage of the pharmaceutical composition of the present invention varies depending on the condition and weight of the patient, the severity of the disease, the form of the drug, and the time, but can be appropriately selected by a person skilled in the art. It is 0.001 mg/kg to 50 mg/kg, and it can be administered once to several times a day as needed.

In addition, the present invention provides a health food composition for preventing or improving inflammation or pain containing the extract of Okchok calligraphy as an active ingredient.

When the composition of the present invention is a health food composition, various nutrients, vitamins, minerals (electrolytes), flavoring agents such as synthetic flavoring agents and natural flavoring agents, coloring agents and thickening agents (cheese, chocolate, etc.), pectic acid and salts thereof , Alginic acid and salts thereof, organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohols, carbonates used in carbonated beverages, and the like. In addition, it may contain pulp for the manufacture of natural fruit juices, synthetic fruit juices and vegetable beverages. These components may be used independently or in combination. In addition, the health food composition may be in the form of any one of meat, sausage, bread, chocolate, candy, snacks, confectionery, pizza, ramen, gum, ice cream, soup, beverage, tea, functional water, drink, alcohol and vitamin complex. I can.

In addition, the health food composition may additionally contain food additives, and the suitability as a food additive is determined according to the general rules and general test methods of the Food Additive Code approved by the Ministry of Food and Drug Safety, unless otherwise specified. It is judged according to standards and standards.

For example, ketones, glycine, potassium citrate, nicotinic acid, chemical synthetic products such as cinnamic acid, dark pigment, licorice extract, crystalline cellulose, high cooling pigment, natural additives such as guar gum, L-glutamic acid And mixed preparations such as sodium preparations, noodle-added alkali preparations, preservative preparations, and tar color preparations.

At this time, the composition according to the present invention added to the food in the process of manufacturing the health food composition can be appropriately added or subtracted, if necessary.

Hereinafter, the present invention will be described in more detail through examples. These examples are only for describing the present invention in more detail, and it will be apparent to those of ordinary skill in the art that the scope of the present invention is not limited by these examples according to the gist of the present invention. .

Example 1: Cell cultivation and treatment with extracts of oyster calligraphy

Test substance 1 (HG-WH140403-2, HGM) was diluted 10-fold and 20-fold with a serum-free medium to prepare each final treatment concentration of 890 ppm and 445 ppm, and then used in the experiment. Test substance 2 (HG-WH140403-3, HGM) was diluted 10-fold and 20-fold with a serum-free medium to prepare each final treatment concentration of 445 ppm and 223 ppm, and then used in the experiment.

Test substance Extraction method Appearance density HG-WH140403-2
(Okchok calligraphy extract) Hot water extraction Pale yellow liquid 0.89% (8,900 ppm) HG-WH140403-3
(Okchok calligraphy extract) Hot water extraction Pale yellow liquid 0.45% (4,500 ppm)

Arbutin (Lot No: BCBH0889V, Sigma-Aldrich Co., U.S.A.) was used as a positive control material. Arbutin was weighed and put into a prepared container, and dimethyl sulfoxide (DMSO, Lot No: K41064631, Merck, Germany) was added as an excipient, followed by stirring. Then, dimethyl sulfoxide was additionally added to obtain a concentration of 25,000 ppm. The prepared positive control material was diluted 100 times with a serum-free medium to obtain a final treatment concentration of 250 ppm, and then used in the experiment.

As a negative control material, a 10% serum-free medium was prepared using tertiary distilled water and used in the experiment.

In order to analyze the melanin production inhibitory effect on the test substance, mouse melanocytes, B16F10 cells (Lot No: 80008, Korea Cell Line Bank) were used. Before treatment with the test substance, 10% fetal bovine serum (FBS, Lot No: 1295617, Gibco, USA) and 1% penicillin-streptomycin (Lot No: 1393883, Gibco) were added to DMEM medium ( Lot No: 0000343372, Lonza, USA) was used, and after treatment of the test substance, a serum-free DMEM medium to which 1% penicillin-streptomycin was added was used.

B16F10 cells were prepared at a concentration of 5 X 10 ⁴ cells/mL, and 3 mL per well of a 6-well plate was dispensed (1.5 Х 10 ⁵ cells/well), and then in a 37°C, 5% CO ₂ incubator for 24 hours. Cultured. After incubation, the culture medium of each well was removed, and 3 mL of the test substance, positive control substance, or negative control substance prepared for each concentration was added to each well. After culturing for 72 hours, the culture medium of each well was removed, and 1 mL of D-PBS was added to wash the cells. After removing the washing solution, 1 mL of lysis buffer (50 mM sodium phosphate buffer containing 1% Triton X-100, pH 6.8) was added to each well, followed by a freezer at -80 to -60°C (DFU-657CL, Operon , Korea) for 2 hours and then left at room temperature for 30 minutes to thaw. After collecting cells from each well and centrifuging (4°C, 13,000 rpm, 30 minutes), the supernatant was used for protein quantification, and the cell pellet was dried for 2 hours or more in a dryer at 60°C, and then used for melanin quantification. .

Example 3: Analysis of the melanin production inhibitory effect of the extract of Okchokseoye

After 0.5 mL of DMSO-NaOH mixed solution [100% DMSO: 1 N NaOH (Lot No: RNBC7103) = 1:9] was added to the dried cell pellet, it was allowed to stand in a dryer at 60° C. for 2 hours. The melanin was dissolved.

Synthetic melanin (Lot No: SLBB1239V, Sigma-Aldrich. Co.) was added to a DMSO-NaOH mixed solution to prepare a concentration of 120 μg/mL (stock solution), and then diluted to 60, 30, 15, 7.5 and It was prepared as a solution (standard solution) at a concentration of 3.75 μg/mL.

A cell pellet lysate obtained by treatment with a test substance, a positive control substance, or a negative control substance, and a standard solution for each concentration were added 0.2 mL to each well of a 96 well plate. Absorbance was measured at 405 nm using an ELISA reader. A standard curve was created using the absorbance of the standard solution, and the absorbance was substituted into the formula obtained from the standard curve to confirm the production of melanin in the cells treated with the test substance, positive control substance or negative control substance. The amount of melanin produced was calculated by correcting the amount of the total protein, and the inhibition rate of melanin formation (IR) was calculated using the following equation.

[Calculation 1]

IR(%) = (BA)/BX 100

A: The amount of melanin in the test substance or positive control substance

B: amount of melanin in negative control material

As a result, referring to Table 2 and FIG. 2, the inhibition rates of melanin production in the test substance 1 treatment group at 445 and 890 ppm concentrations were 91.7 and 89.3%, respectively, and the melanin production in the test substance 2 treatment group at 223 and 445 ppm concentrations. It was confirmed that the inhibition rates were 94.8 and 90.0%, respectively, which significantly inhibited melanin production compared to the negative control group. In addition, it was confirmed that the melanin production inhibition rate in the positive control group (arbutin) at a concentration of 250 ppm was 67.2% compared to the negative control group.

group
density
(ppm) Melanin
(μg/mL) protein
(mg/mL) Melanin/protein
(μg/mg) %IR G1 Negative control material 0 80.5 0.369 218.0 0.0 G2 Test substance 1 445 9.2 0.507 18.1 91.7 890 14.0 0.601 23.2 89.3 G3 Test substance 2 223 5.5 0.486 11.3 94.8 445 7.4 0.342 21.8 90.0 G4 Positive control material 250 13.1 0.381 34.35 67.2

Example 4: Analysis of the anti-inflammatory effect of extract of Okchokseoye

LPS (lipopolysaccharide)-treated mouse macrophages (RAW264.7) were treated with different concentrations of the test substance and cultured for 20 hours to quantify the expression level of IL-6.

As a result, referring to FIG. 3, when compared with the negative control group (0 ppm), it was confirmed that the amount of IL-6 expression in the test substance-treated group decreased in a concentration-dependent manner. LPS is known to promote the expression of inflammatory mediators such as interleukin, TNF-α, iNOS and COX-2, and it was confirmed that it exhibits anti-inflammatory effects by confirming that the expression of IL-6 was suppressed when the test substance was treated.

Example 5: Preparation of Cosmetics Containing the Extract of Okchok Calligraphy

As shown in Table 3 below, a cosmetic product including the extract of Okchok Calligraphy was prepared in the amount of all components and each component.

Briefly, as shown in Fig. 4, components 1 to 7 are first stirred at 50°C, components 8 to 12 are secondarily stirred at 70°C, and components 13 to 19 are third stirred at 50°C And, the components of 20 to 27 were stirred for the fourth time at 50 ℃. Thereafter, the first mixture and the second mixture were mixed and stirred, and the third mixture was added, mixed and stirred, and then cooled to 60°C. Thereafter, the fourth mixture was added, mixed and stirred, cooled to 35° C., and filtered to prepare a cosmetic containing the extract of Okchok Calligraphy.

ingredient %(W/W) One Purified water
(water) 42.43 2 glycerin
(glycerin) 7.50 3 Dimethicone
(dimethicone) 7.50 4 Okchok Calligraphy Extract
(zea mays (corn) silk extract) 7.00 5 Mineral water
(mineral water) 5.50 6 Propylene glycol
(propylene glycol) 5.50 7 Caprylic/Capric Triglyceride
(caprylic/capric triglyceride) 5.00 8 Glyceryl stearate/
Polyethylene glycol-100 stearate
(glyceryl stearate/PEG-100 stearate) 3.00 9 1,2-hexanediol
(1,2-hexanediol) 2 10 Caprylyl Glycol
(caprylyl glycol) 11 Butylene glycol
(butylene glycol) 12 Star anise extract
(Illicium verum (anise) fruit extract) 13 Cetearyl alcohol
(cetearyl alcohol) 1.80 14 Sunflower seed oil
(helianthus annuus (sunflower) seed oil) 1.50 15 Sorbitan stearate
(sorbitan stearate) 0.80 16 Polysorbate 80
(polysorbate 80) 0.80 17 incense
(fragrance) 0.60 18 Acrylate/C10-30 Alkyl Acrylate Crosspolymer
(acrylates/C10-30 alkyl acrylate crosspolymer) 0.58 19 Arginine
(arginine) 0.58 20 Rose extract
(rose extract) 0.30 21 Chamomile flower extract
(chamomilla recutita (matricaria) flower extract) 0.30 22 Rice extract
(oryza sativa (rice) extract) 0.30 23 Aloe barbadensis leaf water 0.30 24 Betaine
(betaine) 0.25 25 Allantoin
(allantoin) 0.20 26 Tocopheryl acetate
(tocopheryl acetate) 0.10 27 Disodium EDT
(disodium EDTA) 0.04

Example 5: Analysis of Cosmetics Containing Okchok Calligraphy Extract

As a result of requesting the KTR Korea Institute of Chemical Convergence Testing to confirm whether the components, microorganisms, and steroids of the cosmetics including the extract of Okchok calligraphy prepared in Example 4 were detected, heavy metals, preservatives, microorganisms as shown in Tables 4 to 6 below , Steroids, etc. were not detected, or trace amounts were detected at a level suitable for cosmetic standards, and it was confirmed that it is harmless and safe to the human body.

Test Items analysis result Exterior Brown liquid Pass smell Specific Pass Specific gravity (d20/20) 0.950~1.050 1.002 pH (25℃) 5.0~7.5 6.1 Heavy metal (ppm) <20 Pass As(ppm) <2 Pass Microorganism (cfu/ml) <100 Pass Preservative (%) Not detected Pass

Test Items unit result Total number of aerobic viable bacteria (number of bacteria) Pcs/ml <20

Test Items unit result Beclomethasone mg/kg Not detected Beclomethasone 17-propionate mg/kg Not detected Beclomethasone 17,21-dipropionate mg/kg Not detected Beclomethasone 21-acetate mg/kg Not detected Beclomethasone 21-hemisuccinate mg/kg Not detected Beclomethasone 21-propionate mg/kg Not detected Betamethasone mg/kg Not detected Betamethasone 17-valerate mg/kg Not detected Betamethasone 17,21-dipropionate mg/kg Not detected Betamethasone 21-acetate mg/kg Not detected Betamethasone 21-hemisuccinate mg/kg Not detected Betamethasone 21-valerate mg/kg Not detected Budesonide mg/kg Not detected Clobetasol propionate mg/kg Not detected

As the specific parts of the present invention have been described in detail above, it is obvious that these specific techniques are only preferred embodiments, and the scope of the present invention is not limited thereto for those of ordinary skill in the art. Accordingly, it will be said that the substantial scope of the present invention is defined by the appended claims and their equivalents.

The scope of the present invention is indicated by the claims to be described later, and all changes or modified forms derived from the meaning and scope of the claims and their equivalent concepts should be interpreted as being included in the scope of the present invention.

Claims (8)

It contains 7.12% by weight of the oyster calligraphy extract obtained by hot water extraction of the flower stalk and pistil of the rice family corn (zea mays linne), based on 100 parts by weight of the composition, and 42.43% by weight of purified water, 7.5% by weight of glycerin, 7.5% by weight of dimethicone, mineral 5.5% by weight of water, 5.5% by weight of propylene glycol and 5% by weight of caprylic/capric triglyceride were first stirred at 50° C.,
Glyceryl stearate/polyethylene glycol-100 stearate 3% by weight, 1,2-hexanediol, caprylyl glycol, butylene glycol, and 2% by weight of star anise extract were added to each 2% by weight at 70°C. Stirring,
Cetearyl alcohol 1.8% by weight, sunflower seed oil 1.5% by weight, sorbitan stearate 0.8% by weight, polysorbate 80 0.8% by weight, fragrance 0.6% by weight, acrylate/C10-30 alkyl acrylate crosspolymer 0.58% by weight , 0.58% by weight of arginine was third stirred at 50°C,
Rose extract, chamomile flower extract, rice extract and aloe vera leaf water each 0.3% by weight, betaine 0.25% by weight, allantoin 0.2% by weight, tocopheryl acetate 0.1% by weight, and disodium EDTA 0.04% by weight were stirred at 50°C for the fourth time After doing,
The mixture of the first stirring and the mixture of the second stirring were mixed and stirred, the mixture of the third stirring was added, mixed and stirred again, and then cooled to 60°C,
A cosmetic composition for improving or whitening skin troubles containing the extract of Okchok Calligraphy, characterized in that the mixture of the fourth agitation is added, mixed and stirred, cooled to 35°C and filtered.
delete delete The cosmetic composition for improving or whitening skin problems according to claim 1, wherein the extract of Okchok calligraphy inhibits the formation of melanin and the expression of IL-6. The method of claim 1, wherein the cosmetic composition is formulated in a formulation selected from the group consisting of lotion, essence, lotion, cream, cleansing foam, powder, pack, gel, ointment, patch, stick, emulsion, and spray. Cosmetic composition for improving or whitening skin troubles. delete delete delete

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