KR102081811B1 - 합성 메신저 rna에 의한 인간 유도 만능 줄기 세포의 피더프리 유래 - Google Patents

합성 메신저 rna에 의한 인간 유도 만능 줄기 세포의 피더프리 유래 Download PDF

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KR102081811B1
KR102081811B1 KR1020147034726A KR20147034726A KR102081811B1 KR 102081811 B1 KR102081811 B1 KR 102081811B1 KR 1020147034726 A KR1020147034726 A KR 1020147034726A KR 20147034726 A KR20147034726 A KR 20147034726A KR 102081811 B1 KR102081811 B1 KR 102081811B1
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지우 왕
루이지 워렌
유후이 니
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알렐 바이오테크놀로지 앤 파마슈티칼스, 인크.
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KR1020147034726A 2012-05-13 2013-05-13 합성 메신저 rna에 의한 인간 유도 만능 줄기 세포의 피더프리 유래 Active KR102081811B1 (ko)

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US201261646292P 2012-05-13 2012-05-13
US61/646,292 2012-05-13
PCT/US2013/040814 WO2013173248A2 (en) 2012-05-13 2013-05-13 Feeder-free derivation of human-induced pluripotent stem cells with synthetic messenger rna

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Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111893140A (zh) 2011-12-05 2020-11-06 菲克特生物科学股份有限公司 用于转染细胞的方法和产品
US8497124B2 (en) 2011-12-05 2013-07-30 Factor Bioscience Inc. Methods and products for reprogramming cells to a less differentiated state
US10119150B2 (en) 2012-05-13 2018-11-06 Allele Biotechnology & Pharmaceuticals, Inc. Feeder-free Derivation of human-induced pluripotent stem cells with synthetic messenger RNA
CA2872688C (en) * 2012-05-13 2023-09-19 Allele Biotechnology And Pharmaceuticals, Inc. Feeder-free derivation of human-induced pluripotent stem cells with synthetic messenger rna
US10155929B2 (en) * 2012-05-13 2018-12-18 Allele Biotechnology & Pharmaceuticals, Inc. Feeder-free derivation of human-induced pluripotent stem cells with synthetic messenger RNA
KR102315098B1 (ko) 2012-11-01 2021-10-21 팩터 바이오사이언스 인크. 세포에서 단백질을 발현시키는 방법들과 생성물들
EP3690056B1 (en) 2014-01-31 2022-12-28 Factor Bioscience Inc. Methods and products for nucleic acid production and delivery
CA2976376A1 (en) 2015-02-13 2016-08-18 Factor Bioscience Inc. Nucleic acid products and methods of administration thereof
US11459560B2 (en) 2015-11-23 2022-10-04 The Regents Of The University Of Colorado Methods and compositions for reprogramming cells
AU2017312113B2 (en) 2016-08-17 2023-05-25 Factor Bioscience Inc. Nucleic acid products and methods of administration thereof
WO2018094114A2 (en) * 2016-11-16 2018-05-24 Allele Biotechnology & Pharmaceuticals, Inc. Induction of pancreatic beta cells by stem cell differentiation with rna
MX2019005766A (es) * 2016-11-16 2019-10-21 Allele Biotechnology & Pharmaceuticals Inc Induccion de hepatocitos por diferenciacion de celulas madre con arn.
KR102122336B1 (ko) * 2018-10-26 2020-06-12 서울시립대학교 산학협력단 세포전환용 rna 나노입자를 이용한 유도만능줄기세포의 제조방법
EP3969571A4 (en) 2019-05-14 2023-05-24 Aleph Farms Ltd. AGGREGATES OF PLURIPOTENT CELLS AND THEIR USE
EP3997059A4 (en) 2019-07-03 2023-12-13 Factor Bioscience Inc. CATIONIC LIPIDS AND THEIR USES
US10501404B1 (en) 2019-07-30 2019-12-10 Factor Bioscience Inc. Cationic lipids and transfection methods
WO2021149822A1 (ja) * 2020-01-24 2021-07-29 アイ ピース， インコーポレイテッド 人工多能性幹細胞の作製方法
JP2022104813A (ja) 2020-12-29 2022-07-11 アイ ピース，インコーポレイテッド リプログラミング因子を導入された細胞の培養方法

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5260432A (en) * 1989-06-22 1993-11-09 Sloan-Kettering Institute For Cancer Research Human gamma retinoic acid receptor DNA
CA3060170A1 (en) * 2008-06-13 2009-12-17 Whitehead Institute For Biomedical Research Programming and reprogramming of cells
EP2192174B1 (en) * 2008-11-21 2015-11-11 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Reprogramming cells toward a pluripotent state
WO2011056971A2 (en) * 2009-11-04 2011-05-12 Cellular Dynamics International, Inc. Episomal reprogramming with chemicals
LT3287525T (lt) * 2009-12-07 2020-02-10 The Trustees Of The University Of Pennsylvania Rnr preparatai, apimantys išgrynintą modifikuotą rnr, skirti ląstelių reprogramavimui
CN102190731B (zh) * 2010-03-09 2016-01-27 中国科学院上海生命科学研究院 用人工转录因子诱导产生多能干细胞
WO2012012708A1 (en) * 2010-07-22 2012-01-26 Regents Of The University Of Minnesota Induced pluripotent stem cells
WO2012012798A2 (en) * 2010-07-23 2012-01-26 University Of South Florida Materials and methods for reduction of protein tau and treatment of neurodegenerative diseases
CN102417894B (zh) * 2011-10-21 2013-06-05 中国科学院广州生物医药与健康研究院 一种提高诱导生成多能性干细胞效率的方法
US10155929B2 (en) * 2012-05-13 2018-12-18 Allele Biotechnology & Pharmaceuticals, Inc. Feeder-free derivation of human-induced pluripotent stem cells with synthetic messenger RNA
CA2872688C (en) * 2012-05-13 2023-09-19 Allele Biotechnology And Pharmaceuticals, Inc. Feeder-free derivation of human-induced pluripotent stem cells with synthetic messenger rna
CN103739718B (zh) * 2014-01-16 2017-02-08 中国科学院动物研究所 用于诱导多能干细胞的融合蛋白及其使用方法

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
Cell Stem Cell, Vol. 7, pp. 549-550(2010)*

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CA2872688C (en) 2023-09-19
WO2013173248A2 (en) 2013-11-21
AU2013263101A1 (en) 2014-11-27
HK1204005A1 (en) 2015-11-06
CN110241087B (zh) 2023-02-03
JP6530452B2 (ja) 2019-06-12
AU2013263101B2 (en) 2019-02-14
JP6448531B2 (ja) 2019-01-09
EP2850179A4 (en) 2016-02-24
JP2015517311A (ja) 2015-06-22
ES2729563T3 (es) 2019-11-04
AU2019203335A1 (en) 2019-05-30
EP2850179B1 (en) 2019-03-20
JP2017184766A (ja) 2017-10-12
CN104520424A (zh) 2015-04-15
WO2013173248A3 (en) 2014-12-18
EP3561054A1 (en) 2019-10-30
AU2019203335B2 (en) 2021-10-21
KR20150035594A (ko) 2015-04-06
EP3561054C0 (en) 2023-07-05
CN104520424B (zh) 2019-06-28
ES2951822T3 (es) 2023-10-25
KR20200021553A (ko) 2020-02-28
CA2872688A1 (en) 2013-11-21
EP3561054B1 (en) 2023-07-05
KR102215413B1 (ko) 2021-02-16
EP2850179A2 (en) 2015-03-25
CN110241087A (zh) 2019-09-17
DK2850179T3 (da) 2019-06-17

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