DK2850179T3 - Feeder-cellefri afledning af human-inducerede pluripotente stamceller med syntetisk messenger-rna - Google Patents
Feeder-cellefri afledning af human-inducerede pluripotente stamceller med syntetisk messenger-rna Download PDFInfo
- Publication number
- DK2850179T3 DK2850179T3 DK13790064.3T DK13790064T DK2850179T3 DK 2850179 T3 DK2850179 T3 DK 2850179T3 DK 13790064 T DK13790064 T DK 13790064T DK 2850179 T3 DK2850179 T3 DK 2850179T3
- Authority
- DK
- Denmark
- Prior art keywords
- human
- messenger rna
- induced pluripotent
- feeder cell
- synthetic messenger
- Prior art date
Links
- 238000009795 derivation Methods 0.000 title 1
- LFULEKSKNZEWOE-UHFFFAOYSA-N propanil Chemical compound CCC(=O)NC1=CC=C(Cl)C(Cl)=C1 LFULEKSKNZEWOE-UHFFFAOYSA-N 0.000 title 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0696—Artificially induced pluripotent stem cells, e.g. iPS
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/111—General methods applicable to biologically active non-coding nucleic acids
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/14—Type of nucleic acid interfering nucleic acids [NA]
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2320/00—Applications; Uses
- C12N2320/30—Special therapeutic applications
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/60—Transcription factors
- C12N2501/602—Sox-2
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/60—Transcription factors
- C12N2501/603—Oct-3/4
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/60—Transcription factors
- C12N2501/605—Nanog
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/60—Transcription factors
- C12N2501/606—Transcription factors c-Myc
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/60—Transcription factors
- C12N2501/608—Lin28
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
- C12N2506/13—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from connective tissue cells, from mesenchymal cells
- C12N2506/1307—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from connective tissue cells, from mesenchymal cells from adult fibroblasts
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2510/00—Genetically modified cells
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- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biomedical Technology (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Wood Science & Technology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Cell Biology (AREA)
- Transplantation (AREA)
- Developmental Biology & Embryology (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Pharmacology & Pharmacy (AREA)
- Medicinal Chemistry (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201261646292P | 2012-05-13 | 2012-05-13 | |
| PCT/US2013/040814 WO2013173248A2 (en) | 2012-05-13 | 2013-05-13 | Feeder-free derivation of human-induced pluripotent stem cells with synthetic messenger rna |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DK2850179T3 true DK2850179T3 (da) | 2019-06-17 |
Family
ID=49548771
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK13790064.3T DK2850179T3 (da) | 2012-05-13 | 2013-05-13 | Feeder-cellefri afledning af human-inducerede pluripotente stamceller med syntetisk messenger-rna |
Country Status (9)
| Country | Link |
|---|---|
| EP (2) | EP3561054B1 (enExample) |
| JP (2) | JP6448531B2 (enExample) |
| KR (2) | KR102081811B1 (enExample) |
| CN (2) | CN104520424B (enExample) |
| AU (2) | AU2013263101B2 (enExample) |
| CA (1) | CA2872688C (enExample) |
| DK (1) | DK2850179T3 (enExample) |
| ES (2) | ES2951822T3 (enExample) |
| WO (1) | WO2013173248A2 (enExample) |
Families Citing this family (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DK3260140T3 (da) | 2011-12-05 | 2021-04-19 | Factor Bioscience Inc | Fremgangsmåder og produkter til transficering af celler |
| US8497124B2 (en) | 2011-12-05 | 2013-07-30 | Factor Bioscience Inc. | Methods and products for reprogramming cells to a less differentiated state |
| EP3561054B1 (en) * | 2012-05-13 | 2023-07-05 | Allele Biotechnology And Pharmaceuticals, Inc. | Feeder-free derivation of human-induced pluripotent stem cells with synthetic messenger rna |
| US10155929B2 (en) | 2012-05-13 | 2018-12-18 | Allele Biotechnology & Pharmaceuticals, Inc. | Feeder-free derivation of human-induced pluripotent stem cells with synthetic messenger RNA |
| US10119150B2 (en) | 2012-05-13 | 2018-11-06 | Allele Biotechnology & Pharmaceuticals, Inc. | Feeder-free Derivation of human-induced pluripotent stem cells with synthetic messenger RNA |
| BR122019025681B1 (pt) | 2012-11-01 | 2023-04-18 | Factor Bioscience Inc | Método para inserir uma sequência de ácido nucleico em uma localização segura de um genoma de uma célula |
| WO2015117021A1 (en) | 2014-01-31 | 2015-08-06 | Factor Bioscience Inc. | Methods and products for nucleic acid production and delivery |
| EP3543339A1 (en) | 2015-02-13 | 2019-09-25 | Factor Bioscience Inc. | Nucleic acid products and methods of administration thereof |
| EP4470544A3 (en) * | 2015-11-23 | 2025-06-18 | The Regents of the University of Colorado, a body corporate | Methods and compositions for reprogramming cells |
| CN109803977B (zh) | 2016-08-17 | 2023-03-17 | 菲克特生物科学股份有限公司 | 核酸产品及其施用方法 |
| CA3043372A1 (en) | 2016-11-16 | 2018-05-24 | Allele Biotechnology & Pharmaceuticals, Inc. | Induction of pancreatic beta cells by stem cell differentiation with rna |
| AU2017363142B2 (en) * | 2016-11-16 | 2024-05-30 | Allele Biotechnology & Pharmaceuticals, Inc. | Induction of hepatocytes by stem cell differentiation with RNA |
| KR102122336B1 (ko) * | 2018-10-26 | 2020-06-12 | 서울시립대학교 산학협력단 | 세포전환용 rna 나노입자를 이용한 유도만능줄기세포의 제조방법 |
| EP3969571A4 (en) | 2019-05-14 | 2023-05-24 | Aleph Farms Ltd. | AGGREGATES OF PLURIPOTENT CELLS AND THEIR USE |
| WO2021003462A1 (en) | 2019-07-03 | 2021-01-07 | Factor Bioscience Inc. | Cationic lipids and uses thereof |
| US10501404B1 (en) | 2019-07-30 | 2019-12-10 | Factor Bioscience Inc. | Cationic lipids and transfection methods |
| US20230053060A1 (en) * | 2020-01-24 | 2023-02-16 | I Peace, Inc. | Method for manufacturing induced pluripotent stem cells |
| JP2022104813A (ja) | 2020-12-29 | 2022-07-11 | アイ ピース，インコーポレイテッド | リプログラミング因子を導入された細胞の培養方法 |
Family Cites Families (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5260432A (en) * | 1989-06-22 | 1993-11-09 | Sloan-Kettering Institute For Cancer Research | Human gamma retinoic acid receptor DNA |
| CN105671065A (zh) * | 2008-06-13 | 2016-06-15 | 怀特黑德生物医学研究所 | 细胞编程和重编程 |
| EP2881461A1 (en) * | 2008-11-21 | 2015-06-10 | Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. | Reprogramming cells toward a pluripotent state |
| ES2539487T3 (es) * | 2009-11-04 | 2015-07-01 | Cellular Dynamics International, Inc. | Reprogramación episómica con compuestos químicos |
| EP3287525B1 (en) * | 2009-12-07 | 2019-10-23 | The Trustees of The University of Pennsylvania | Rna preparations comprising purified modified rna for reprogramming cells |
| CN102190731B (zh) * | 2010-03-09 | 2016-01-27 | 中国科学院上海生命科学研究院 | 用人工转录因子诱导产生多能干细胞 |
| WO2012012708A1 (en) * | 2010-07-22 | 2012-01-26 | Regents Of The University Of Minnesota | Induced pluripotent stem cells |
| US8940945B2 (en) * | 2010-07-23 | 2015-01-27 | University Of South Florida | Materials and methods for reduction of protein tau and treatment of neurodegenerative diseases |
| CN102417894B (zh) * | 2011-10-21 | 2013-06-05 | 中国科学院广州生物医药与健康研究院 | 一种提高诱导生成多能性干细胞效率的方法 |
| US10155929B2 (en) * | 2012-05-13 | 2018-12-18 | Allele Biotechnology & Pharmaceuticals, Inc. | Feeder-free derivation of human-induced pluripotent stem cells with synthetic messenger RNA |
| EP3561054B1 (en) * | 2012-05-13 | 2023-07-05 | Allele Biotechnology And Pharmaceuticals, Inc. | Feeder-free derivation of human-induced pluripotent stem cells with synthetic messenger rna |
| CN103739718B (zh) * | 2014-01-16 | 2017-02-08 | 中国科学院动物研究所 | 用于诱导多能干细胞的融合蛋白及其使用方法 |
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2013
- 2013-05-13 EP EP19163001.1A patent/EP3561054B1/en active Active
- 2013-05-13 ES ES19163001T patent/ES2951822T3/es active Active
- 2013-05-13 CN CN201380025010.4A patent/CN104520424B/zh active Active
- 2013-05-13 CA CA2872688A patent/CA2872688C/en active Active
- 2013-05-13 CN CN201910468645.0A patent/CN110241087B/zh active Active
- 2013-05-13 AU AU2013263101A patent/AU2013263101B2/en active Active
- 2013-05-13 DK DK13790064.3T patent/DK2850179T3/da active
- 2013-05-13 KR KR1020147034726A patent/KR102081811B1/ko active Active
- 2013-05-13 JP JP2015511803A patent/JP6448531B2/ja active Active
- 2013-05-13 WO PCT/US2013/040814 patent/WO2013173248A2/en not_active Ceased
- 2013-05-13 EP EP13790064.3A patent/EP2850179B1/en active Active
- 2013-05-13 ES ES13790064T patent/ES2729563T3/es active Active
- 2013-05-13 KR KR1020207004988A patent/KR102215413B1/ko active Active
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2017
- 2017-07-14 JP JP2017137655A patent/JP6530452B2/ja active Active
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2019
- 2019-05-13 AU AU2019203335A patent/AU2019203335B2/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| EP2850179B1 (en) | 2019-03-20 |
| KR102081811B1 (ko) | 2020-02-26 |
| CA2872688A1 (en) | 2013-11-21 |
| AU2013263101A1 (en) | 2014-11-27 |
| AU2013263101B2 (en) | 2019-02-14 |
| KR102215413B1 (ko) | 2021-02-16 |
| JP2017184766A (ja) | 2017-10-12 |
| ES2729563T3 (es) | 2019-11-04 |
| CA2872688C (en) | 2023-09-19 |
| KR20200021553A (ko) | 2020-02-28 |
| CN110241087A (zh) | 2019-09-17 |
| CN104520424B (zh) | 2019-06-28 |
| EP3561054A1 (en) | 2019-10-30 |
| AU2019203335A1 (en) | 2019-05-30 |
| EP2850179A4 (en) | 2016-02-24 |
| HK1204005A1 (en) | 2015-11-06 |
| ES2951822T3 (es) | 2023-10-25 |
| WO2013173248A2 (en) | 2013-11-21 |
| EP3561054B1 (en) | 2023-07-05 |
| CN104520424A (zh) | 2015-04-15 |
| JP6448531B2 (ja) | 2019-01-09 |
| KR20150035594A (ko) | 2015-04-06 |
| EP2850179A2 (en) | 2015-03-25 |
| CN110241087B (zh) | 2023-02-03 |
| AU2019203335B2 (en) | 2021-10-21 |
| JP2015517311A (ja) | 2015-06-22 |
| WO2013173248A3 (en) | 2014-12-18 |
| JP6530452B2 (ja) | 2019-06-12 |
| EP3561054C0 (en) | 2023-07-05 |
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