KR102043891B1 - 개선된 유전적 안전성을 가지는 감소된 게놈 박테리아 - Google Patents

개선된 유전적 안전성을 가지는 감소된 게놈 박테리아 Download PDF

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KR102043891B1
KR102043891B1 KR1020147012964A KR20147012964A KR102043891B1 KR 102043891 B1 KR102043891 B1 KR 102043891B1 KR 1020147012964 A KR1020147012964 A KR 1020147012964A KR 20147012964 A KR20147012964 A KR 20147012964A KR 102043891 B1 KR102043891 B1 KR 102043891B1
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mds42
reduced
coli
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KR20140076628A (ko
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프레데릭 알. 블래트너
발린트 씨소르고
기요르기 포스파이
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스캐랩 게노믹스, 엘엘씨
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/08—Reducing the nucleic acid content
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
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    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/185—Escherichia
    • C12R2001/19—Escherichia coli

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KR1020147012964A 2011-10-20 2012-10-19 개선된 유전적 안전성을 가지는 감소된 게놈 박테리아 Active KR102043891B1 (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201161549375P 2011-10-20 2011-10-20
US61/549,375 2011-10-20
PCT/US2012/061027 WO2013059595A1 (en) 2011-10-20 2012-10-19 Reduced genome bacteria with improved genetic stability

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KR20140076628A KR20140076628A (ko) 2014-06-20
KR102043891B1 true KR102043891B1 (ko) 2019-11-12

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US (1) US9340791B2 (enExample)
EP (1) EP2768943B1 (enExample)
JP (2) JP6151704B2 (enExample)
KR (1) KR102043891B1 (enExample)
CN (1) CN104024398B (enExample)
AU (1) AU2012325917B2 (enExample)
CA (1) CA2850842C (enExample)
WO (1) WO2013059595A1 (enExample)

Families Citing this family (7)

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Publication number Priority date Publication date Assignee Title
US9340791B2 (en) * 2011-10-20 2016-05-17 Scarab Genomics, Llc Reduced genome bacteria with improved genetic stability
JP6545676B2 (ja) * 2013-11-14 2019-07-17 スカラブ ゲノミクス， エルエルシー 改善された代謝能力を有する細菌
KR102315246B1 (ko) * 2014-03-03 2021-10-21 스캐랩 게노믹스, 엘엘씨 대장균에서 재조합 ｃｒｍ197의 향상된 생산
KR102351231B1 (ko) 2015-04-02 2022-01-14 스캐랩 게노믹스, 엘엘씨 감소된 게놈 박테리아의 연장된 연속 흐름 배양을 위한 물질 및 방법
CN108587992B (zh) * 2018-04-10 2022-03-15 安徽大学 一种高效分泌表达抗VEGF-Fab抗体片段的高产菌株及其构建方法
CN111019877B (zh) * 2019-12-31 2022-04-19 浙江工业大学 一种产l-半胱氨酸的基因工程菌、构建方法及应用
WO2022256427A1 (en) * 2021-06-01 2022-12-08 University Of Virginia Patent Foundation Minicells from highly genome reduced escherichia coli: cytoplasmic and surface expression of recombinant proteins and incorporation in the minicells

Citations (2)

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Publication number Priority date Publication date Assignee Title
JP2009533027A (ja) 2006-04-07 2009-09-17 ウイスコンシン アラムニ リサーチ ファンデーション 挿入配列を含まない細菌
WO2010093708A1 (en) 2009-02-12 2010-08-19 Scarab Genomics Llc Compositions comprising reduced genome bacteria for use in treatment of sepsis

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US5578464A (en) 1989-10-31 1996-11-26 Schering Corporation E. coli secretory strains
US5747662A (en) 1995-03-01 1998-05-05 Genentech, Inc. Methods and compositions for secretion of heterologous polypeptides
US6022952A (en) 1998-04-01 2000-02-08 University Of Alberta Compositions and methods for protein secretion
US6989265B2 (en) * 2002-01-23 2006-01-24 Wisconsin Alumni Research Foundation Bacteria with reduced genome
US8039243B2 (en) 2002-01-23 2011-10-18 Wisconsin Alumni Research Foundation Insertion sequence-free bacteria
US20060270043A1 (en) 2002-01-23 2006-11-30 Blattner Frederick R Bacteria with reduced genome
US7303906B2 (en) 2002-09-06 2007-12-04 Wisconsin Alumni Research Foundation Competent bacteria
CA2626900C (en) * 2005-08-20 2013-07-23 Scarab Genomics, Llc Reduced genome e. coli
US9340791B2 (en) * 2011-10-20 2016-05-17 Scarab Genomics, Llc Reduced genome bacteria with improved genetic stability

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2009533027A (ja) 2006-04-07 2009-09-17 ウイスコンシン アラムニ リサーチ ファンデーション 挿入配列を含まない細菌
WO2010093708A1 (en) 2009-02-12 2010-08-19 Scarab Genomics Llc Compositions comprising reduced genome bacteria for use in treatment of sepsis

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
The EMBO Journal (2000) Vol.19, No.22, p.6259-6265

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Publication number Publication date
AU2012325917A1 (en) 2014-04-17
JP6539691B2 (ja) 2019-07-03
KR20140076628A (ko) 2014-06-20
HK1201560A1 (en) 2015-09-04
US9340791B2 (en) 2016-05-17
JP6151704B2 (ja) 2017-06-21
AU2012325917B2 (en) 2018-04-12
CA2850842A1 (en) 2013-04-25
CN104024398B (zh) 2016-10-05
JP2017169582A (ja) 2017-09-28
US20140302555A1 (en) 2014-10-09
CN104024398A (zh) 2014-09-03
EP2768943A1 (en) 2014-08-27
WO2013059595A1 (en) 2013-04-25
EP2768943B1 (en) 2017-06-21
CA2850842C (en) 2021-06-08
JP2014530625A (ja) 2014-11-20

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