JP6151704B2 - 遺伝的安定性が向上したゲノム縮小化細菌 - Google Patents

遺伝的安定性が向上したゲノム縮小化細菌 Download PDF

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JP6151704B2
JP6151704B2 JP2014537289A JP2014537289A JP6151704B2 JP 6151704 B2 JP6151704 B2 JP 6151704B2 JP 2014537289 A JP2014537289 A JP 2014537289A JP 2014537289 A JP2014537289 A JP 2014537289A JP 6151704 B2 JP6151704 B2 JP 6151704B2
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genome
gene
bacterium
strain
genes
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JP2014530625A5 (enExample
JP2014530625A (ja
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フレデリック アール． ブラットナー，
フレデリック アール． ブラットナー，
バリント ツォルゴ，
バリント ツォルゴ，
ジォルジュ ポスファイ，
ジォルジュ ポスファイ，
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スカラブ ゲノミクス， エルエルシー
スカラブ ゲノミクス， エルエルシー
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/08—Reducing the nucleic acid content
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/185—Escherichia
    • C12R2001/19—Escherichia coli

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
JP2014537289A 2011-10-20 2012-10-19 遺伝的安定性が向上したゲノム縮小化細菌 Expired - Fee Related JP6151704B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201161549375P 2011-10-20 2011-10-20
US61/549,375 2011-10-20
PCT/US2012/061027 WO2013059595A1 (en) 2011-10-20 2012-10-19 Reduced genome bacteria with improved genetic stability

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JP2017103668A Division JP6539691B2 (ja) 2011-10-20 2017-05-25 遺伝的安定性が向上したゲノム縮小化細菌

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JP2014530625A JP2014530625A (ja) 2014-11-20
JP2014530625A5 JP2014530625A5 (enExample) 2015-11-05
JP6151704B2 true JP6151704B2 (ja) 2017-06-21

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JP2014537289A Expired - Fee Related JP6151704B2 (ja) 2011-10-20 2012-10-19 遺伝的安定性が向上したゲノム縮小化細菌
JP2017103668A Active JP6539691B2 (ja) 2011-10-20 2017-05-25 遺伝的安定性が向上したゲノム縮小化細菌

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US (1) US9340791B2 (enExample)
EP (1) EP2768943B1 (enExample)
JP (2) JP6151704B2 (enExample)
KR (1) KR102043891B1 (enExample)
CN (1) CN104024398B (enExample)
AU (1) AU2012325917B2 (enExample)
CA (1) CA2850842C (enExample)
WO (1) WO2013059595A1 (enExample)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2017169582A (ja) * 2011-10-20 2017-09-28 スカラブ ゲノミクス， エルエルシー 遺伝的安定性が向上したゲノム縮小化細菌

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3068887B1 (en) 2013-11-14 2019-10-30 Scarab Genomics LLC Bacteria with improved metabolic capacity
JP6473460B2 (ja) * 2014-03-03 2019-02-20 スカラブ ゲノミクス， エルエルシー 大腸菌における組換えｃｒｍ１９７の産生強化
CA2981583C (en) * 2015-04-02 2022-11-15 Scarab Genomics, Llc Continuous fermentation process for production of biological product using reduced genome escherichia coli
CN108587992B (zh) * 2018-04-10 2022-03-15 安徽大学 一种高效分泌表达抗VEGF-Fab抗体片段的高产菌株及其构建方法
CN111019877B (zh) * 2019-12-31 2022-04-19 浙江工业大学 一种产l-半胱氨酸的基因工程菌、构建方法及应用
US20240309390A1 (en) * 2021-06-01 2024-09-19 University Of Virginia Patent Foundation Minicells from highly genome reduced escherichia coli: cytoplasmic and surface expression of recombinant proteins and incorporation in the minicells

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5578464A (en) 1989-10-31 1996-11-26 Schering Corporation E. coli secretory strains
US5747662A (en) 1995-03-01 1998-05-05 Genentech, Inc. Methods and compositions for secretion of heterologous polypeptides
US6022952A (en) 1998-04-01 2000-02-08 University Of Alberta Compositions and methods for protein secretion
US8119365B2 (en) * 2002-01-23 2012-02-21 Wisconsin Alumni Research Foundation Insertion sequence-free bacteria
US6989265B2 (en) * 2002-01-23 2006-01-24 Wisconsin Alumni Research Foundation Bacteria with reduced genome
US20060270043A1 (en) 2002-01-23 2006-11-30 Blattner Frederick R Bacteria with reduced genome
US8039243B2 (en) 2002-01-23 2011-10-18 Wisconsin Alumni Research Foundation Insertion sequence-free bacteria
US7303906B2 (en) 2002-09-06 2007-12-04 Wisconsin Alumni Research Foundation Competent bacteria
CA2626900C (en) * 2005-08-20 2013-07-23 Scarab Genomics, Llc Reduced genome e. coli
EP2396015B1 (en) * 2009-02-12 2013-04-17 Scarab Genomics LLC Compositions comprising reduced genome bacteria for use in treatment of sepsis
EP2768943B1 (en) * 2011-10-20 2017-06-21 Scarab Genomics LLC Reduced genome bacteria with improved genetic stability

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2017169582A (ja) * 2011-10-20 2017-09-28 スカラブ ゲノミクス， エルエルシー 遺伝的安定性が向上したゲノム縮小化細菌

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Publication number Publication date
CA2850842C (en) 2021-06-08
US20140302555A1 (en) 2014-10-09
KR102043891B1 (ko) 2019-11-12
HK1201560A1 (en) 2015-09-04
CN104024398A (zh) 2014-09-03
CA2850842A1 (en) 2013-04-25
JP2017169582A (ja) 2017-09-28
AU2012325917B2 (en) 2018-04-12
EP2768943A1 (en) 2014-08-27
WO2013059595A1 (en) 2013-04-25
JP6539691B2 (ja) 2019-07-03
EP2768943B1 (en) 2017-06-21
CN104024398B (zh) 2016-10-05
JP2014530625A (ja) 2014-11-20
AU2012325917A1 (en) 2014-04-17
KR20140076628A (ko) 2014-06-20
US9340791B2 (en) 2016-05-17

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