KR102018863B1 - 개선된 발현을 위해 박테리아 추출물 중 선택 단백질의 단백질분해 불활성화 - Google Patents

개선된 발현을 위해 박테리아 추출물 중 선택 단백질의 단백질분해 불활성화 Download PDF

Info

Publication number
KR102018863B1
KR102018863B1 KR1020157012291A KR20157012291A KR102018863B1 KR 102018863 B1 KR102018863 B1 KR 102018863B1 KR 1020157012291 A KR1020157012291 A KR 1020157012291A KR 20157012291 A KR20157012291 A KR 20157012291A KR 102018863 B1 KR102018863 B1 KR 102018863B1
Authority
KR
South Korea
Prior art keywords
protein
seq
cell
ompt1
amino acids
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
KR1020157012291A
Other languages
English (en)
Korean (ko)
Other versions
KR20150064209A (ko
Inventor
크리스토퍼 디. 타노스
크리스토퍼 제이. 머레이
준하오 양
헤더 스티븐슨
Original Assignee
서트로 바이오파마, 인크.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 서트로 바이오파마, 인크. filed Critical 서트로 바이오파마, 인크.
Publication of KR20150064209A publication Critical patent/KR20150064209A/ko
Application granted granted Critical
Publication of KR102018863B1 publication Critical patent/KR102018863B1/ko
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4702—Regulators; Modulating activity
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • C07K14/245—Escherichia (G)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/06—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies from serum
    • C07K16/065—Purification, fragmentation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/32—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against translation products of oncogenes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/04—Preparation or injection of sample to be analysed
    • G01N30/06—Preparation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/50—Fusion polypeptide containing protease site
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Pathology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • General Physics & Mathematics (AREA)
  • Oncology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Plant Pathology (AREA)
  • Toxicology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
KR1020157012291A 2012-10-12 2013-10-08 개선된 발현을 위해 박테리아 추출물 중 선택 단백질의 단백질분해 불활성화 Active KR102018863B1 (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201261713245P 2012-10-12 2012-10-12
US61/713,245 2012-10-12
PCT/US2013/063804 WO2014058830A1 (en) 2012-10-12 2013-10-08 Proteolytic inactivation of select proteins in bacterial extracts for improved expression

Publications (2)

Publication Number Publication Date
KR20150064209A KR20150064209A (ko) 2015-06-10
KR102018863B1 true KR102018863B1 (ko) 2019-09-05

Family

ID=49447839

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1020157012291A Active KR102018863B1 (ko) 2012-10-12 2013-10-08 개선된 발현을 위해 박테리아 추출물 중 선택 단백질의 단백질분해 불활성화

Country Status (14)

Country Link
US (3) US9650621B2 (enExample)
EP (1) EP2906592B1 (enExample)
JP (3) JP6421122B2 (enExample)
KR (1) KR102018863B1 (enExample)
CN (1) CN104837863B (enExample)
AU (1) AU2013329464C1 (enExample)
CA (1) CA2887355C (enExample)
DK (1) DK2906592T3 (enExample)
ES (1) ES2694683T3 (enExample)
HU (1) HUE041721T2 (enExample)
IL (1) IL238095B (enExample)
PL (1) PL2906592T3 (enExample)
SG (1) SG11201502875VA (enExample)
WO (1) WO2014058830A1 (enExample)

Families Citing this family (35)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR102018863B1 (ko) * 2012-10-12 2019-09-05 서트로 바이오파마, 인크. 개선된 발현을 위해 박테리아 추출물 중 선택 단백질의 단백질분해 불활성화
CA2909516C (en) * 2013-04-19 2023-03-07 Sutro Biopharma, Inc. Expression of biologically active proteins in a bacterial cell-free synthesis system using cell extracts with elevated levels of exogenous chaperones
WO2016004024A1 (en) * 2014-07-02 2016-01-07 Sutro Biopharma, Inc. High growth capacity auxotrophic escherichia coli and methods of use
CN108779446B (zh) * 2016-01-13 2023-04-21 新英格兰生物实验室公司 T7 rna聚合酶的热稳定变体
KR20190103256A (ko) 2016-12-30 2019-09-04 수트로박스, 인코포레이티드 비자연 아미노산을 갖는 폴리펩타이드-항원 접합체
US11951165B2 (en) 2016-12-30 2024-04-09 Vaxcyte, Inc. Conjugated vaccine carrier proteins
CN106801047A (zh) * 2016-12-30 2017-06-06 武汉金开瑞生物工程有限公司 一种通过无细胞蛋白合成系统制备半胱氨酸蛋白酶的方法
CA3069321A1 (en) 2017-07-11 2019-01-17 Synthorx, Inc. Incorporation of unnatural nucleotides and methods thereof
US20200181220A1 (en) 2017-08-03 2020-06-11 Synthorx, Inc. Cytokine conjugates for the treatment of proliferative and infectious diseases
US10835590B2 (en) 2017-10-12 2020-11-17 Vaxcyte, Inc. Periodontitis vaccine and related compositions and methods of use
TW202442671A (zh) 2018-02-26 2024-11-01 美商欣爍克斯公司 Il-15結合物及其用途
CN118147029A (zh) * 2018-07-11 2024-06-07 阿克蒂姆治疗有限公司 工程化的免疫刺激性细菌菌株及其用途
CN108949796A (zh) * 2018-07-27 2018-12-07 张家港市华天药业有限公司 一种用于合成谷胱甘肽的重组菌及谷胱甘肽的合成方法
CN109055291A (zh) * 2018-07-31 2018-12-21 张家港市华天药业有限公司 用于合成谷胱甘肽的重组菌及谷胱甘肽的合成方法
MA54195A (fr) 2018-11-08 2022-02-16 Synthorx Inc Conjugués d'interleukine 10 et leurs utilisations
US20200246467A1 (en) 2019-02-06 2020-08-06 Synthorx, Inc. Il-2 conjugates and methods of use thereof
MX2021012120A (es) 2019-04-02 2021-11-03 Vaxcyte Inc Sintesis optimizada libre de celulas del antigeno b del plasmido de invasion y composiciones y metodos de uso relacionados.
EP4013454A1 (en) 2019-08-15 2022-06-22 Synthorx, Inc. Immuno oncology combination therapies with il-2 conjugates
CA3150082A1 (en) 2019-09-10 2021-03-18 Jerod PTACIN Il-2 conjugates and methods of use to treat autoimmune diseases
AU2020380275A1 (en) 2019-11-04 2022-04-14 Synthorx, Inc. Interleukin 10 conjugates and uses thereof
US12351850B2 (en) * 2020-04-30 2025-07-08 Sutro Biopharma, Inc. Methods of producing full-length antibodies using E. coli
JP2023531509A (ja) 2020-06-25 2023-07-24 シンソークス， インコーポレイテッド Ｉｌ－２コンジュゲートおよび抗ｅｇｆｒ抗体を用いる免疫腫瘍学併用療法
EP4211156A1 (en) 2020-09-14 2023-07-19 Sutro Biopharma, Inc. Method for large scale production of antibodies using a cell-free protein synthesis system
EP4225375A1 (en) 2020-10-09 2023-08-16 Synthorx, Inc. Immuno oncology therapies with il-2 conjugates
BR112023006364A2 (pt) 2020-10-09 2023-05-09 Synthorx Inc Terapia de combinação de imuno-oncologia com conjugados de il-2 e pembrolizumabe
WO2022174101A1 (en) 2021-02-12 2022-08-18 Synthorx, Inc. Skin cancer combination therapy with il-2 conjugates and cemiplimab
WO2022174102A1 (en) 2021-02-12 2022-08-18 Synthorx, Inc. Lung cancer combination therapy with il-2 conjugates and an anti-pd-1 antibody or antigen-binding fragment thereof
CN113322267B (zh) * 2021-03-15 2024-01-05 天津大学 系统在提高非天然氨基酸的插入效率中的应用
WO2022256534A1 (en) 2021-06-03 2022-12-08 Synthorx, Inc. Head and neck cancer combination therapy comprising an il-2 conjugate and pembrolizumab
EP4452327A1 (en) 2021-12-20 2024-10-30 Synthorx, Inc. Head and neck cancer combination therapy comprising an il-2 conjugate and pembrolizumab
WO2023122750A1 (en) 2021-12-23 2023-06-29 Synthorx, Inc. Cancer combination therapy with il-2 conjugates and cetuximab
WO2024136899A1 (en) 2022-12-21 2024-06-27 Synthorx, Inc. Cancer therapy with il-2 conjugates and chimeric antigen receptor therapies
TW202444349A (zh) 2023-03-20 2024-11-16 美商欣爍克斯公司 使用ｉｌ－２綴合物之癌症療法
WO2025015041A1 (en) * 2023-07-12 2025-01-16 University Of Washington Rna-only, positive control target for clinical diagnostic testing
WO2025158385A1 (en) 2024-01-25 2025-07-31 Genzyme Corporation Pegylated il-2 for suppressing adaptive immune response to gene therapy

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU758633B2 (en) * 1999-03-04 2003-03-27 Asubio Pharma Co., Ltd. Method for controlling cleavage by OmpT protease
WO2002059293A2 (en) 2001-01-25 2002-08-01 Forster Anthony C Process and compositions for peptide, protein and peptidomimetic synthesis
AU2003259912B2 (en) 2002-08-19 2008-10-23 The Board Of Trustees Of The Leland Stanford Junior University Improved methods of in vitro protein synthesis
DE10336705A1 (de) * 2003-08-06 2005-03-10 Rina Netzwerk Rna Technologien Verfahren zur Herstellung eines Lysats zur zellfreien Proteinbiosynthese
AU2004276687B2 (en) * 2003-09-30 2011-07-14 Asubio Pharma Co., Ltd. Method of cleaving polypeptide by using OmpT protease mutant
ATE468406T1 (de) 2005-02-02 2010-06-15 Univ Bayreuth Esterasen zur überwachung der proteinbiosynthese in vitro
CA2624290A1 (en) 2005-10-12 2007-04-26 Scripps Research Institute Selective posttranslational modification of phage-displayed polypeptides
JP5249194B2 (ja) * 2006-03-16 2013-07-31 ザ スクリプス リサーチ インスティチュート 非天然アミノ酸フェニルセレノシステインを含有するタンパク質の遺伝的にプログラムされた発現
EP2376647B1 (en) 2009-01-12 2014-03-05 Sutro Biopharma, Inc. Mono charging system for selectively introducing non-native amino acids into proteins using an in vitro protein synthesis system
KR102018863B1 (ko) 2012-10-12 2019-09-05 서트로 바이오파마, 인크. 개선된 발현을 위해 박테리아 추출물 중 선택 단백질의 단백질분해 불활성화

Also Published As

Publication number Publication date
AU2013329464A1 (en) 2015-05-14
HUE041721T2 (hu) 2019-05-28
AU2013329464B2 (en) 2017-07-27
US20150259664A1 (en) 2015-09-17
EP2906592A1 (en) 2015-08-19
CA2887355C (en) 2023-03-07
JP2015531407A (ja) 2015-11-02
KR20150064209A (ko) 2015-06-10
SG11201502875VA (en) 2015-06-29
AU2013329464C1 (en) 2018-02-15
IL238095B (en) 2019-11-28
EP2906592B1 (en) 2018-10-03
US20170283469A1 (en) 2017-10-05
CN104837863B (zh) 2018-06-19
US11261219B2 (en) 2022-03-01
JP6421122B2 (ja) 2018-11-07
US20200062807A1 (en) 2020-02-27
US9650621B2 (en) 2017-05-16
US10450353B2 (en) 2019-10-22
ES2694683T3 (es) 2018-12-26
JP2021003134A (ja) 2021-01-14
CA2887355A1 (en) 2014-04-17
WO2014058830A1 (en) 2014-04-17
CN104837863A (zh) 2015-08-12
DK2906592T3 (en) 2018-12-10
PL2906592T3 (pl) 2019-07-31
IL238095A0 (en) 2015-05-31
JP2018029621A (ja) 2018-03-01

Similar Documents

Publication Publication Date Title
KR102018863B1 (ko) 개선된 발현을 위해 박테리아 추출물 중 선택 단백질의 단백질분해 불활성화
JP7292442B2 (ja) 改変アミノアシルｔＲＮＡ合成酵素およびその用途
KR102289258B1 (ko) 상승된 수준의 외인성 샤페론을 갖는 세포 추출물을 이용한 박테리아 세포 비함유 합성 시스템에서의 생물학적 활성 단백질의 발현
CN102348807B (zh) 利用体外蛋白合成体系将非天然氨基酸引入蛋白的单装载体系
US20220243244A1 (en) Compositions and methods for in vivo synthesis of unnatural polypeptides
CN107614689A (zh) 用于将非天然氨基酸并入蛋白质中的平台
Cui et al. Semisynthetic tRNA complement mediates in vitro protein synthesis
US20220228148A1 (en) Eukaryotic semi-synthetic organisms
WO2020243026A1 (en) Methods and compositions for manufacturing polynucleotides
US11584921B2 (en) Non-standard amino acid containing compositions and uses thereof
WO2014194129A2 (en) Cell-free synthetic incorporation of non-natural amino acids into proteins
US10731148B2 (en) Use of lambda-gam protein in ribosomal display technology
US20240384267A1 (en) Compositions and methods for multiplex decoding of quadruplet codons
WO2015115661A1 (ja) アゾール誘導体骨格を有するペプチドの製造方法
Exner Incorporation of novel noncanonical amino acids in model proteins using rational and evolved variants of Methanosarcina mazei pyrrolysyl-tRNA synthetase
HK1167003A (en) Mono charging system for selectively introducing non-native amino acids into proteins using an in vitro protein synthesis system
KR20160025661A (ko) 디제너러시 리프로그래밍을 통한 비천연 단백질 합성 방법

Legal Events

Date Code Title Description
PA0105 International application

Patent event date: 20150511

Patent event code: PA01051R01D

Comment text: International Patent Application

PG1501 Laying open of application
A201 Request for examination
PA0201 Request for examination

Patent event code: PA02012R01D

Patent event date: 20181008

Comment text: Request for Examination of Application

PA0302 Request for accelerated examination

Patent event date: 20181008

Patent event code: PA03022R01D

Comment text: Request for Accelerated Examination

E902 Notification of reason for refusal
PE0902 Notice of grounds for rejection

Comment text: Notification of reason for refusal

Patent event date: 20181107

Patent event code: PE09021S01D

E902 Notification of reason for refusal
PE0902 Notice of grounds for rejection

Comment text: Notification of reason for refusal

Patent event date: 20190328

Patent event code: PE09021S01D

E701 Decision to grant or registration of patent right
PE0701 Decision of registration

Patent event code: PE07011S01D

Comment text: Decision to Grant Registration

Patent event date: 20190530

PR0701 Registration of establishment

Comment text: Registration of Establishment

Patent event date: 20190830

Patent event code: PR07011E01D

PR1002 Payment of registration fee

Payment date: 20190830

End annual number: 3

Start annual number: 1

PG1601 Publication of registration
PR1001 Payment of annual fee

Payment date: 20220718

Start annual number: 4

End annual number: 4

PR1001 Payment of annual fee

Payment date: 20230718

Start annual number: 5

End annual number: 5

PR1001 Payment of annual fee

Payment date: 20240722

Start annual number: 6

End annual number: 6