ES2694683T3 - Inactivación proteolítica de proteínas seleccionadas en extractos bacterianos para mejorar la expresión - Google Patents
Inactivación proteolítica de proteínas seleccionadas en extractos bacterianos para mejorar la expresión Download PDFInfo
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- ES2694683T3 ES2694683T3 ES13779699.1T ES13779699T ES2694683T3 ES 2694683 T3 ES2694683 T3 ES 2694683T3 ES 13779699 T ES13779699 T ES 13779699T ES 2694683 T3 ES2694683 T3 ES 2694683T3
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Classifications
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- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
- C07K14/245—Escherichia (G)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/06—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies from serum
- C07K16/065—Purification, fragmentation
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
- C07K16/32—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against translation products of oncogenes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/04—Preparation or injection of sample to be analysed
- G01N30/06—Preparation
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/50—Fusion polypeptide containing protease site
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
- C12Y304/21—Serine endopeptidases (3.4.21)
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
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- General Engineering & Computer Science (AREA)
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- Microbiology (AREA)
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- Chemical Kinetics & Catalysis (AREA)
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- General Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Gastroenterology & Hepatology (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Oncology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Plant Pathology (AREA)
- Toxicology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Applications Claiming Priority (3)
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| US201261713245P | 2012-10-12 | 2012-10-12 | |
| US201261713245P | 2012-10-12 | ||
| PCT/US2013/063804 WO2014058830A1 (en) | 2012-10-12 | 2013-10-08 | Proteolytic inactivation of select proteins in bacterial extracts for improved expression |
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| ES2694683T3 true ES2694683T3 (es) | 2018-12-26 |
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| ES13779699.1T Active ES2694683T3 (es) | 2012-10-12 | 2013-10-08 | Inactivación proteolítica de proteínas seleccionadas en extractos bacterianos para mejorar la expresión |
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| CN (1) | CN104837863B (enExample) |
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| ES (1) | ES2694683T3 (enExample) |
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| PL (1) | PL2906592T3 (enExample) |
| SG (1) | SG11201502875VA (enExample) |
| WO (1) | WO2014058830A1 (enExample) |
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| JP6421122B2 (ja) * | 2012-10-12 | 2018-11-07 | ストロ バイオファーマ， インコーポレイテッド | 改善された発現のための、細菌抽出物中の選択タンパク質のタンパク質分解不活性化 |
| HUE059565T2 (hu) * | 2013-04-19 | 2022-11-28 | Sutro Biopharma Inc | Biológiailag aktív fehérjék expressziója bakteriális sejtmentes szintézisrendszerben, emelkedett exogén chaperonszintû sejtextraktumok segítségével |
| US10316322B2 (en) * | 2014-07-02 | 2019-06-11 | Sutro Biopharma, Inc. | High growth capacity auxotrophic Escherichia coli and methods of use |
| CN106801047A (zh) * | 2016-12-30 | 2017-06-06 | 武汉金开瑞生物工程有限公司 | 一种通过无细胞蛋白合成系统制备半胱氨酸蛋白酶的方法 |
| US11951165B2 (en) | 2016-12-30 | 2024-04-09 | Vaxcyte, Inc. | Conjugated vaccine carrier proteins |
| KR20240110660A (ko) | 2016-12-30 | 2024-07-15 | 박사이트, 인코포레이티드 | 비자연 아미노산을 갖는 폴리펩타이드-항원 접합체 |
| AR112756A1 (es) | 2017-07-11 | 2019-12-11 | Synthorx Inc | Incorporación de nucleótidos no naturales, y su método |
| MX2020001336A (es) | 2017-08-03 | 2020-08-20 | Synthorx Inc | Conjugados de citoquina para el tratamiento de enfermedades autoinmunes. |
| JP7258870B2 (ja) | 2017-10-12 | 2023-04-17 | バックスサイト・インコーポレイテッド | 歯周炎ワクチンおよび関連組成物ならびに使用方法 |
| TWI834636B (zh) | 2018-02-26 | 2024-03-11 | 美商欣爍克斯公司 | Il-15結合物及其用途 |
| WO2020014543A2 (en) * | 2018-07-11 | 2020-01-16 | Actym Therapeutics, Inc. | Engineered immunostimulatory bacterial strains and uses thereof |
| CN108949796A (zh) * | 2018-07-27 | 2018-12-07 | 张家港市华天药业有限公司 | 一种用于合成谷胱甘肽的重组菌及谷胱甘肽的合成方法 |
| CN109055291A (zh) * | 2018-07-31 | 2018-12-21 | 张家港市华天药业有限公司 | 用于合成谷胱甘肽的重组菌及谷胱甘肽的合成方法 |
| JP7636323B2 (ja) | 2018-11-08 | 2025-02-26 | シンソークス， インコーポレイテッド | インターロイキン１０コンジュゲートおよびその使用 |
| JP7702872B2 (ja) | 2019-02-06 | 2025-07-04 | シンソークス， インコーポレイテッド | Ｉｌ－２コンジュゲートおよびその使用方法 |
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| WO2000052193A1 (fr) * | 1999-03-04 | 2000-09-08 | Suntory Limited | Procede de controle de clivage par la protease de l'ompt |
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| DE10336705A1 (de) * | 2003-08-06 | 2005-03-10 | Rina Netzwerk Rna Technologien | Verfahren zur Herstellung eines Lysats zur zellfreien Proteinbiosynthese |
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| DE602006014362D1 (de) | 2005-02-02 | 2010-07-01 | Univ Bayreuth | Esterasen zur überwachung der proteinbiosynthese in vitro |
| KR20080069993A (ko) * | 2005-10-12 | 2008-07-29 | 더 스크립스 리서치 인스티튜트 | 파지-디스플레이된 폴리펩티드의 선별적인 번역후 변형 |
| MX2008011669A (es) | 2006-03-16 | 2008-09-22 | Scripps Research Inst | Expresion programada geneticamente de proteinas que contienen el aminoacido no natural fenilselenocisteina. |
| CN102348807B (zh) | 2009-01-12 | 2015-04-15 | 苏特罗生物制药公司 | 利用体外蛋白合成体系将非天然氨基酸引入蛋白的单装载体系 |
| JP6421122B2 (ja) | 2012-10-12 | 2018-11-07 | ストロ バイオファーマ， インコーポレイテッド | 改善された発現のための、細菌抽出物中の選択タンパク質のタンパク質分解不活性化 |
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| DK2906592T3 (en) | 2018-12-10 |
| CN104837863A (zh) | 2015-08-12 |
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| SG11201502875VA (en) | 2015-06-29 |
| JP6421122B2 (ja) | 2018-11-07 |
| AU2013329464B2 (en) | 2017-07-27 |
| JP2021003134A (ja) | 2021-01-14 |
| CA2887355A1 (en) | 2014-04-17 |
| WO2014058830A1 (en) | 2014-04-17 |
| US10450353B2 (en) | 2019-10-22 |
| KR20150064209A (ko) | 2015-06-10 |
| US20200062807A1 (en) | 2020-02-27 |
| IL238095B (en) | 2019-11-28 |
| PL2906592T3 (pl) | 2019-07-31 |
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