KR101987554B1 - Method of the sprout barley mixture tea increased content of antioxidant or active ingredient of Anti-diabetic with eliminating the unpleasant odor of sprout barley, powder of silkworm and leaves of Cudrania tricuspidata - Google Patents

Abstract

The present invention relates to a method for preparing a mixture of shoot barley, silkworm powder, and curd leaf blended tea, wherein the content of active ingredient of antioxidant or hypoglycemic agent is removed by removing the offspring of silkworm, silkworm and silkworm.
The method of the present invention for eliminating the foot of the bud barley, the silkworm powder and the unpleasant odor of the silkworm,
2 to 5 liters of a mixture of 50 to 80% of purified water and 20 to 50% of alcohol are mixed with 8 to 20 g of barley powder, stirred at room temperature, allowed to stand for 30 to 60 minutes, and then subjected to primary centrifugation and left for 30 to 60 minutes Followed by secondary centrifugation and then collecting along a supernatant portion;
1 to 5 liters of a solution containing 30 to 80% of purified water and 20 to 70% of alcohol, and 4 to 20 g of silkworm powder are mixed, stirred at room temperature, allowed to stand for 30 to 60 minutes, centrifuged and then collected along the upper part Wow;
1 ~ 5 liters of a mixture of 30 ~ 95% of purified water and 5 ~ 70% of alcohol, stirring 8 ~ 80g of leaf, stirring at room temperature, leaving for 30 ~ 60 minutes, centrifuging and collecting along the upper part ; And
The three supernatants of the above step were collected and the hot water extract was recovered by evaporating the ethanol at a temperature of 74 ° C or below for 20-30 minutes and then evaporating ethanol at a temperature of 77-82 ° C for 60-180 minutes for 60-180 minutes in an ethanol- The final supernatant of the hot-water extract is collected and the concentration is calculated to make a mixed tea.
The mixed tea according to the present invention can be prepared without any sensation because it is a health food which is difficult to make as a beverage because of the inhaled and unpleasant work, and it is possible to make a mixture of the seed barley, silkworm, The present invention can contribute to the health of adult patients or diabetic patients by simply making them into a mixed tea or a mixture of sprout barley and cilantro leaves. In particular, the present invention shows excellent effects on DPPH scavenging activity and? -Glucosidase inhibitory activity of antioxidant activity.

Description

TECHNICAL FIELD The present invention relates to a method for producing a sprout barley mixture, an antioxidant or an active ingredient, and a method for producing the sprout barley mixture, which comprises increasing the antioxidant or hypoglycemic active ingredient content, ingredient of Anti-diabetic with eliminating the unpleasant odor of sprout barley, powder of silkworm and leaves of Cudrania tricuspidata}

The present invention relates to a method for preparing a sprout barley, a silkworm powder and a curd leaf blended tea having increased antioxidant or hypoglycemic active ingredient content,

More specifically, it is a method for removing unpleasant odor of silkworm meal and silkworm powder by using the extract of alcohol and partial hot water instead of the whole hot water extraction. It is a solution of 50 to 80% of purified water and 20 to 50% of alcohol ~ 5 liters, 8 ~ 20g of barley flour were mixed, stirred at room temperature, allowed to stand for 30 ~ 60 minutes, centrifuged (3,000 rpm)

In the same manner, 1 to 5 liters of a mixture of 30 to 80% of purified water and 20 to 70% of alcohol was mixed with 4 to 20 g of silkworm powder, and the mixture was stirred at room temperature for 30 to 60 minutes, centrifuged (3,000 rpm) supernatant portion,

1 to 5 liters of a mixture of 30 to 95% of purified water and 5 to 70% of alcohol is mixed with 8 to 80 g of leaf, and boiled at a temperature of 70 to 90 ° C for 15 to 30 minutes. The centrifuged upper layer of the above- The extracts were prepared by mixing the extracts of leaf juice with cucumber leaves and boiling at 78.3 ℃ for 60 ~ 180 min to evaporate and recover the extracts. The final supernatant of the hot water extract was collected and the concentration was calculated.

The mixed tea according to the present invention is effective for antioxidant and antidiabetic purposes, and can be prepared without any feeling because it is a healthy food which has been very difficult to be made into a beverage because of the inhaled and unpleasant work, and the snack barley, silkworm, , Which is made by simple blending of ginseng barley, silkworm mixed tea or sprout barley and cucumber leaf mixture tea to improve the health of adult patients and diabetic patients. The present invention relates to a method for preparing a mixture of sprout barley, silkworm powder, and cucumber leaf blended tea with increased antioxidant or hypoglycemic active ingredient content.

It has been known as a nutrient food source since it has high content of inorganic components such as calcium, magnesium and potassium as well as a high content of vitamin B1 and vitamin C.

In addition, it contains a functional secondary metabolite such as antioxidant, anti-inflammatory, anti-cancer saponarin, Lutonarin and Isovitexin (Benedet JA et al, J. Agric , 2007, 55, 5499-5504). As a material for health functional foods and medicines, its industrial applicability is increasing.

In Japan and the United States, dried products of dried barley leaves are developed and sold as health foods.

In addition, many functional substances such as policosanol (Patent Application No. 10-2011-116385) effective for metabolic syndrome such as hypertension, diabetes, and lipid metabolism abnormality are contained, and thus the industrial applicability as a health functional food and medicine material It is getting bigger.

On the other hand, skin aging, various cancers, and inflammation were found to be the main cause of reactive oxygen species (ROS).

Therefore, studies are being conducted to develop an antioxidant capable of removing such harmful active oxygen as a material for many health functional foods and the like.

It has been reported that soybean barley, a young barley leaf, contains a large amount of polyphenols, and these polyphenols have an excellent antioxidative effect for removing harmful active oxygen which is harmful to human body.

The present invention relates to a method for inhibiting neuraminidase activity and a pharmaceutical composition for the prevention and treatment of influenza virus infection diseases, Methods for producing wheat or barley tea, beverage for diabetic patients, analysis of water-soluble phenol compounds of barley leaves, tofu containing barley leaf powder, and the like.

In addition, a study on the effect of cholesterol on the improvement of cholesterol by using barley, the effect of barley on the lipid content and hepatic lipid metabolism-related enzyme activity in high fat fed mice, and the antioxidative effect and hyperlipemia inhibitory effect of barley extract using rabbit atherogenic effect model .

The silkworms eat the young leaves of the mulberry tree by the explanation of the silkworm powder, and go through the 5th order and turn the silkworm cocoon. Silkworms may be classified as spring silkworms, summer silkworms, or autumn silkworms depending on the time of occurrence.

In one room, the larvae of the silkworm are infected with white staphylococcus and are stigmatized. They are called "white mackerel," and they are used as medicinal herbs. The larvae of the larvae, pupa, eggs and silkworms are also used as medicine.

Many methods have been attempted to solve the inconsistency between the insulin content of elevated blood glucose and blood after the meal, including the control of the drug administration time, the development of a new drug administration route, and the development of new formulations that promote drug absorption But has not yet achieved satisfactory results.

In addition, a variety of methods for solving the hyperglycemia problem after the meal have been developed, a method using an inhibitor of alpha -glycosidase, which exhibits activity of delaying the absorption of glucose in the small intestine, has been developed, The drug has come to be proved through.

In this way, administration of the? -Glycosidase inhibitory substance in the small intestine can control rapidly rising blood glucose levels after meal and can solve a large increase and decrease in blood glucose levels commonly seen in diabetic patients. Of course, unnecessary insulin The inhibition of freezes can also help to prevent the onset of other circulatory complications due to hyperinsulinemia (Patent No. 2002-71334).

In the technique of the present invention Because of the complexity of the production process in the process of mixing tea, the extract obtained from hot water extraction tea or silkworm powder at 30 to 70% ethanol will be used.

Mulberry leaves are widely known to be effective for diabetes if they are taken by car according to folk remedies. In recent years, not only are they effective for various adult diseases such as hypertension and arteriosclerosis, but also have the effect of releasing heavy metals in the body, promoting diuretic, It is being revealed.

It has been found that mulberry leaves have various effects as described above. Recently, various attempts have been made to utilize the functional effects of mulberry leaves and it has been known that they have the same action effect as oral diabetic drugs called? -GI. It is a medicinal herb with a very high value of commodity.

Mulberry leaves have various effects because they contain various functional ingredients. Mulberry leaves have been analyzed to contain more than 50 kinds of various minerals.

Mulberry leaves contain 20 ~ 40% of crude protein and contain about 24 kinds of various amino acids. Among them, 3% of alanine and aspartic acid which are effective in relieving hangover, improve brain function and remove cholesterol. And 1.2% and 0.7% respectively of serine and tyrosine, respectively.

Mulberry leaf contains many minerals. Calcium contains 60 times of cabbage, iron is 160 times of radish, and phosphorus contains 10 times of radish. Compared with green tea, calcium is 6 times and iron is 2 times , Sodium is 13 times and potassium is 1.4 times.

According to recent research results, GABA ( r- aminobutyric acid), known as a blood pressure lowering and neurotransmitter, is contained in mulberry leaves containing 250 mg per 100 g of dry weight of mulberry leaves, (rutin) ingredient is also known to contain about three times the amount of green tea.

In addition, it has been known that DNJ (deoxynojirimycin), which inhibits blood glucose absorption, is present only in mulberry leaves. Additional animal experiments have shown that there are blood glucose and hypotensive substances, cholesterol-lowering substances, antioxidants and anticancer substances in mulberry leaves (See Lee, Wanju et al., Herbal Medicine Review, 30 (2): 123-129, 1999).

In particular, Cudrania tricuspidata (Carr.) Bureau ex Lavallee) is a common name for the mulberry tree. Good. It is called a bamboo mulberry tree. It grows in many countries in Asia. It has a different name in each region of the country. It is a deciduous tree belonging to the mulberry family.

It was used as a hypotensive agent, a therapeutic agent for tuberculosis, an antipyretic agent, a disinfectant, a disinfectant, a diuretic agent, a hemostatic agent, a gustatory agent and the like as an antifungal agent for an athlete's foot depending on the parts such as roots, bark, stem, And is used for chronic indigestion due to weakness of digestive organs.

In addition, the fruit of the tree is rounded with a diameter of 2.5cm, ripen in red from September to October, and flesh is sweet and edible.

Its fruit contains useful organic substances such as vitamin B, B1, B2, vitamin C, linoleic acid, glucose, maltose, fructose, malic acid and lemon acid. Stem shells are effective for lung cancer, colon cancer, skin cancer, Has been reported to be contained in a large amount.

It is known from ancient times that it is used as a medicament because it contains planovoid which is known to have an anticancer effect. )

In addition, it is known that the amount of Rutin is about 70 times higher than that of ordinary mulberry leaves.

In most of the prior art methods using the cucumber leaves, it is used as a raw material of the car as it is processed with the cucumber leaves, or it is used as a raw material of the tea by pulverizing the processed cucumber leaves. The development of the technology has not yet been achieved.

We want to develop a new method to produce a product that keeps the unique functionality of cilantro leaves while eliminating the leaves of cilantro leaves to prevent foreign body sensation or rejection when consumed and to promote the health of consumers.

While there are few prior arts directly related to the discomfort and exhaustion of the present invention in the prior art, the prior art related to antioxidant and anti-diabetic are as follows.

Korean Patent Laid-Open Publication No. 10-2016-55748 An antioxidant or hypoglycemic active ingredient content of the roasted barley tea of the Rural Development Administration is increased by hot water extraction of the roasted barley prepared at 180-250 ° C, The present invention relates to a food composition for antioxidant, antiinflammation or hypoglycemia containing an extract as an active ingredient,

Korean Patent No. 10-396986 A method for producing an? -Glucosidase inhibitory substance from a silkworm powder of Rural Development Administration is a method for producing a hypoglycemic substance from a silkworm powder, wherein silkworm powder is extracted with ethanol and then filtered, Ion exchange column chromatography is carried out to separate 1-deoxynojirimycin, which is an excellent substance for hypoglycemic action.

Korean Patent Laid-Open Publication No. 10-2006-95038 In order to provide a beverage composition which is easy to take, as well as being effective in removing hangover when making a hangover and small beverage composition,

A mixture consisting of 10 to 20% by weight of rhizome, 20 to 30% by weight of moisture, 15 to 40% by weight of root, 10 to 30% by weight of roots and 5 to 20% by weight of powder is mixed with purified water, Deg.] C for 2 to 3 hours, filtering the upper liquid, boiling at 100 to 110 [deg.] C for 20 minutes to 30 minutes, and sterilizing.

As a result, it is possible to provide a snack-beverage composition which is free from unpleasant odor and imbalance and which is useful for relieving hangover without the addition of a saccharide ingredient which has the same health effect as the snail's mite, moisture root, root muscle, . Among them, it is presumed that the mugwort component and the like cancel the unpleasant odor and the sense of imitation,

Korean Patent Laid-Open Publication No. 10-2016-132288 discloses a method for producing perilla perilla extract from which the extract is removed, and more specifically, a process for producing perilla perilla And a pre-treatment step of hot-air drying the fresh perilla to remove the inhaled extract.

The present invention has been developed to further improve antioxidant and hypoglycemic functional food substitute beverages by eliminating inhaled and unpleasant odors.

The subject prototype of the present invention is a beverage consisting of sprout barley, silkworm powder,

It is a technique to increase the content of blood sugar-lowering active ingredients by removing the inhaled and unpleasant odor of buds of barley, silkworm, and cucumber leaves. It provides a beverage product that maintains the health of diabetic patients with antioxidant fatigue reduction and elimination of active oxygen. It was developed to allow.

(1) Korean Patent Laid-Open No. 10-2016-55748 entitled "Sprout barley with increased antioxidant or hypoglycemic active ingredient content" (2) Korean Patent No. 10-396986 "Method for producing alpha glucosidase inhibitor from silkworm powder" (3) Korean Patent Laid-Open No. 10-2006-95038 entitled " (4) Korean Patent Laid-Open No. 10-2016-132288 "Method for producing raw perilla extract with removed foot &

The present invention has been developed in order to overcome the above-mentioned problems of the prior art, and it is an object of the present invention to provide a method for preventing and treating diseases caused by sprouts, barley, silkworm, The method of manufacturing a cedar leaf blend car,

It is made up of bean curd, silkworm, and cucumber leaf blended tea products, and it is composed of beverage form. It is easy to drink even though antioxidant and blood glucose lowering active ingredient is contained in existing bud bud, silkworm, Although it was not easy to make,

The mixed tea of the present invention is effective for antioxidant and antidiabetic purposes, and can produce a healthy food such as bud, barley, silkworm, and silkworm blend tea, which have been difficult to make as a beverage because of the in-

It is an object of the present invention to apply this method so as to make it simple as a mixture of bud, barley powder, silkworm barley, and cucumber leaf mixture to contribute to the health of adult patients or diabetic patients.

In order to accomplish the above object, the present invention provides a method for producing a sprout barley, a silkworm powder, and a curd leaf blended tea having increased antioxidant or hypoglycemic active ingredient content by eliminating unpleasant odor of silkworm powder,
2 to 5 liters of a mixture of 50 to 80% of purified water and 20 to 50% of alcohol are mixed with 8 to 20 g of barley powder, stirred at room temperature, allowed to stand for 30 to 60 minutes, and then subjected to primary centrifugation and left for 30 to 60 minutes Followed by secondary centrifugation and then collecting along a supernatant portion;
1 to 5 liters of a solution containing 30 to 80% of purified water and 20 to 70% of alcohol, and 4 to 20 g of silkworm powder are mixed, stirred at room temperature, allowed to stand for 30 to 60 minutes, centrifuged and then collected along the upper part Wow;
1 ~ 5 liters of a mixture of 30 ~ 95% of purified water and 5 ~ 70% of alcohol, stirring 8 ~ 80g of leaf, stirring at room temperature, leaving for 30 ~ 60 minutes, centrifuging and collecting along the upper part ; And
The three supernatants of the above step were collected and the hot water extract was recovered by evaporating the ethanol at a temperature of 74 ° C or below for 20-30 minutes and then evaporating ethanol at a temperature of 77-82 ° C for 60-180 minutes for 60-180 minutes in an ethanol- And the final supernatant of the hot-water extract is collected and the concentration is calculated to produce a mixed tea.

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As described above, in the mixed car manufactured by the manufacturing method of the present invention,

Antioxidant and anti-diabetic effect, and it is possible to make a healthy food such as bud barley, silkworm powder and cucumber leaf blended tea, which have been difficult to make as a beverage due to inhaled and unpleasant work,

The method of manufacturing the mixed tea according to the present invention can be applied simply to make a mixture of sprouts barley, silkworm powder, sprouts barley, and cilantro leaf mixture to contribute to the health of adult patients or diabetic patients.

In addition, the method of the present invention for producing a mixed tea is effective for eliminating the sensation by using a method of extracting sprout barley, silkworm powder, cucumber leaf and unpleasant odor using a 10 to 70% alcohol fraction extraction method.

In addition, the method for producing a mixed tea of the present invention exhibits an excellent effect on the DPPH scavenging activity of antioxidant activity and the? -Glucosidase inhibitory activity of glucose hydrolysis inhibitory effect.

BRIEF DESCRIPTION OF THE DRAWINGS Figure 1 is a manufacturing process diagram showing a preferred embodiment of the present invention.

In order to accomplish the above object, the present invention relates to a method for manufacturing a tea bud of a bud or barb, a method for preparing a bud of a bud or a barbell, will be.

More particularly, the present invention relates to a method for eliminating unpleasant odors in shoots and shoots of silkworms and silkworms by using alcohol extract and partial hot water extraction instead of whole hot water extraction, which is effective for human body obtained from plant powder or extract,

2 to 5 liters of a solution containing 50 to 80% of purified water and 20 to 50% of alcohol are mixed with 8 to 20 g of barley powder, stirred at room temperature, allowed to stand for 30 to 60 minutes, primary centrifuged at 3,000 rpm, After incubation for ~ 60 min, secondary centrifugation at 3,000 rpm is carried out along the supernatant.

In the same manner, 1 to 5 liters of a solution containing 30 to 80% of purified water and 20 to 70% of alcohol was mixed with 4 to 20 g of silkworm powder, stirred at room temperature, left for 30 to 60 minutes, centrifuged at 3,000 rpm, (supernatant).

After mixing 1 ~ 5 liters of a mixture of 30 ~ 95% of purified water with 5 ~ 70% of alcohol and stirring 8 ~ 80g of leaf, the mixture was stirred at room temperature and left for 30 ~ 60 minutes. After centrifuging at 3,000 rpm, supernatant.

After collecting all three supernatants, the ethanol extract was recovered by evaporating the ethanol at a temperature of 74 ° C or below for 20-30 minutes and then evaporating ethanol at a temperature of 77-82 ° C for 60-180 minutes. The final supernatant of the extract is collected and the concentration is calculated to make a mixed tea.

< Manufacturing example  1>: sprouts barley, Silk powder  Mixed car

2 to 5 liters of a solution containing 50 to 80% of purified water and 20 to 50% of alcohol are mixed with 8 to 20 g of barley flour and incubated at room temperature for 30 to 60 minutes. The mixture is subjected to primary centrifugation at 3,000 rpm, After standing for 60 minutes, it is centrifuged at 3,000 rpm and collected along the supernatant portion.

In the same manner, 1 to 5 liters of a solution containing 30 to 80% of purified water and 20 to 70% of alcohol are mixed with 4 to 20 g of silkworm powder, stirred at room temperature, and left for 30 to 60 minutes. The mixture is centrifuged at 3,000 rpm, supernatant.

After collecting all of the two supernatants, ethanol was evaporated at a temperature of 74 ° C or below for 20-30 minutes at a temperature of 74 ° C or lower, followed by evaporation of ethanol at a temperature of 77-82 ° C for 60-180 minutes. The hot water extract was recovered, The final supernatant of the extract was collected and the concentration was calculated to prepare a mixed tea.

< Manufacturing example  2>: shoot barley, Cedar leaf  Mixed car

2 to 5 liters of a solution containing 50 to 80% of purified water and 20 to 50% of alcohol are mixed with 8 to 20 g of barley powder, stirred at room temperature, allowed to stand for 30 to 60 minutes and then subjected to primary centrifugation at 3,000 rpm. After standing for 60 minutes, centrifuge again at 3,000 rpm and collect along supernatant.

1 ~ 5 liters of a mixture of 30 ~ 95% of purified water and 5 ~ 70% of alcohol was mixed with 8 ~ 80g of leaf, and the mixture was stirred at room temperature and left for 30 ~ 60 minutes, centrifuged at 3,000 rpm, We collect the parts along.

After collecting all of the two supernatants, the supernatant was collected in an ethanol recovery apparatus at a temperature of 74 ° C or lower for 20-30 minutes, and ethanol was evaporated at a temperature of 77-82 ° C for 60-180 minutes to recover the hot water extract. The final supernatant was collected and the concentration was calculated to prepare a mixed tea.

< Manufacturing example  3>: shoot barley, Cedar leaf , Silk powder  Mixed car

2 to 5 liters of a solution containing 50 to 80% of purified water and 20 to 50% of alcohol are mixed with 8 to 20 g of barley powder, stirred at room temperature, allowed to stand for 30 to 60 minutes and then subjected to primary centrifugation at 3,000 rpm. After standing for 60 minutes, secondary centrifugation at 3,000 rpm is carried out along the supernatant.

In the same manner, 1 to 5 liters of a solution containing 30 to 80% of purified water and 20 to 70% of alcohol are mixed with 4 to 20 g of silkworm powder, stirred at room temperature, and left for 30 to 60 minutes. The mixture is centrifuged at 3,000 rpm, supernatant.

The mixture was mixed with 1 ~ 5 liters of a mixture of 30 ~ 95% of purified water and 5 ~ 70% of alcohol, and 8 ~ 80g of leaf was mixed with the mixture. The mixture was stirred at room temperature for 30 ~ 60 minutes, centrifuged at 3,000 rpm, We collect the parts along.

After collecting all three supernatants, the ethanol extract was recovered by evaporating the ethanol at a temperature of 74 ° C or below for 20-30 minutes and then evaporating ethanol at a temperature of 77-82 ° C for 60-180 minutes. The final supernatant of the extract is collected and the concentration is calculated to make a mixed tea.

In the case of the present invention using the alcohol fraction extraction process from the above production example,

1) Compared with hot water extraction, in - and out - of - puffing decreased to less than 90% level.

2) The concentration of effective substances such as deoxynojirimycin increased.

< Test Example  1>: Sensory evaluation

(30, 40, and 50 years old male and 2 female) who were well trained as a mixture of ginseng barley, silkworm barley, silkworm powder, Point: excellent, 3 or more: excellent, less than 3: normal) are shown in Table 1 below.

From the above results, the appearance, texture and smell of the ethanol fraction extract were superior to those of the control (pure hot water extract).

< Test Example  2>: DPPH  Radical Scatters

1) Sample preparation

(1) Powder after grinding (20 mesh) is obtained.

② Dissolve 10 g of sample and 100 ml of 80% ethanol.

③ Extract at 70 ℃ for 3 hours.

④ After filtration (55mm), freeze the supernatant.

(5) 1 g of the lyophilized sample and 100 ml of distilled water were added to the sample for analysis.

2) Used equipment

microplate reader (VERSAmax microplate reader, S / N BN 01910, Molecular Devices, USA).

3) Test method

3-1. DPPH radical scavenging test

1) The reagent is DPPH (fw 394.3, sigma-D9132, USA) and STD. : Ascorbic acid (Sigma, USA).

2) Experimental method

① Add 160 ㎕ of DPPH solution dissolved in MeOH and add 40 ㎕ of sample.

② Reaction in cow for 30 minutes.

③ Absorbance was measured at 515 nm.

As a result of the antioxidative activity analysis of the control (sample No. 1) and the experimental group (sample No. 2), the antioxidative activity of the control was about 49% at the concentration of 2000 μg / μl and about 97% As shown in Fig.

The antioxidative activity of the extracts was similar to that of the conventional antioxidant, ascorbic acid 50 ㎍ / ㎕ (about 94%). The antioxidative activity was about 8.2% higher than that of the control.

3-2. α-lucosidase inhibitory activity test

1) Reagent

α-lucosidase Type from bakers yeast (Sigma-G5003, 21 units / mg, USA)

- 4-Nitrophenyl [alpha] -D-glucopyranoside (Sigma, USA)

- sodium carbonate (daejung, KOREA)

- STD. : Acarbose (Sigma, USA)

2) Experimental method

① Add 10 μl of sample and 30 μl of 1 unit / ml α-glucosidase.

② Reaction at 37 ℃ for 10 minutes.

3) Add 30 μl of 1 mM 4-Nitrophenyl α-D-glucopyranoside.

④ Reaction at 37 ℃ for 20 minutes

⑤ Add 130 ㎕ of 1M sodium carbonate

⑥ Measure absorbance at 405nm.

Analysis of antidiabetic activity of α-glucosidase activity of the control (sample No. 1) and the experimental group (sample No. 2) showed that about 35% of the control group and about 44% of the antidiabetic group at the concentration of 500 μg / Inhibitory activity and showed activity in a concentration dependent manner.

It was confirmed that the antidiabetic inhibitory activity of the experimental group was similar in the concentration of acarbose125 / / 기존 (about 46%), which is known as a conventional antidiabetic agent. In addition, the experimental group showed about 19% higher antidiabetic activity than the control group.

Antioxidant activity, antidiabetic activity and glucosease inhibitory activity were investigated. The results of the analysis are as follows.

① Antioxidant activity of DPPH radical scavenging activity was analyzed and the antioxidative activity of the sample (fermented pellet) was 48.2% higher than that of the control.

The antioxidant activity was also similar to ascorbic acid, which is known as the antioxidant.

② The anti-diabetic activity of α-glucosidase was about 38% higher than that of the control.

In addition, acarbose, which is known as a conventional antidiabetic agent, showed similar anti - diabetic activity to the sample.

The method using alcohol extraction and partial hydrothermal extraction instead of the general hot water extraction of the present invention can be applied not only to the offspring of bud and barley and the offensive odor of silkworm powder but also to the herb or herb containing other disgusting aroma, It is highly effective in removing weakness and other senses, and therefore it is likely to be used industrially.

Furthermore, the extracts of the alcoholic extracts of the present invention exhibit superior effects on the functional properties than the hydrothermal extracts of plants having antioxidative activity, antidiabetic activity, and sugar chain inhibitory activity such as bud, barberry, silkworm leaf and silkworm powder.

Claims (3)

2 to 5 liters of a mixture of 50 to 80% of purified water and 20 to 50% of alcohol are mixed with 8 to 20 g of barley powder, stirred at room temperature, allowed to stand for 30 to 60 minutes, and then subjected to primary centrifugation and left for 30 to 60 minutes Followed by secondary centrifugation and then collecting along a supernatant portion;
1 to 5 liters of a solution containing 30 to 80% of purified water and 20 to 70% of alcohol, and 4 to 20 g of silkworm powder are mixed, stirred at room temperature, allowed to stand for 30 to 60 minutes, centrifuged and then collected along the upper part Wow;
1 ~ 5 liters of a mixture of 30 ~ 95% of purified water and 5 ~ 70% of alcohol, stirring 8 ~ 80g of leaf, stirring at room temperature, leaving for 30 ~ 60 minutes, centrifuging and collecting along the upper part ; And
The three supernatants of the above step were collected and the hot water extract was recovered by evaporating the ethanol at a temperature of 74 ° C or below for 20-30 minutes and then evaporating ethanol at a temperature of 77-82 ° C for 60-180 minutes for 60-180 minutes in an ethanol- Characterized in that the final supernatant of the hot-water extract is collected and the concentration is calculated to produce a mixed tea.
A method for preparing a mixture of sprout barley, silkworm powder, and cucumber leaf blended with increased content of antioxidant or hypoglycemic active ingredient, removing the off - flavor of silkworm barley and silkworm powder.
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