KR101982228B1 - Pigs raising method with a feed composition comprising fermented aloe saponaria - Google Patents

Abstract

The present invention relates to a pig breeding method characterized by feeding a fermented product obtained by adding a fermented product of aloe saponaria to a conventional pig feed, wherein the aloe saponaria fermented product is obtained by collecting raw aloe saponaria from water And the mixture was aged at a temperature of 25-30 ° C for 6 months to be aged. The aged sugar solution was added to the prepared material at a ratio of 4.5 to 5.5 times by volume Of water was added thereto, and the mixture was adjusted to pH 4.0 by adding acetic acid. Then, the slurry was sterilized at about 121 ° C for 15 minutes and 5% by weight of the suspension of P. gonorrhoeae was inoculated therein. After obtaining the primary fermented product of Aloe saponaria, about 1.5 ton of octane, about 1.5 ton of wheat, about 2 ton of raw aloe saponaria crushed stock solution and about 200 kg of zeolite were mixed with 100 kg of the primary fermentation product Not prepare the feed composition is prepared, and then inoculated again with the Aloe sand paper or the Syrian primary fermentation compared to 5% by weight of the prepared animal feed composition was 10 days aerobic fermentation conditions at a temperature of 5-30 ℃.

Description

FIELD OF THE INVENTION [0001] The present invention relates to a method for breeding pigs using a feed containing fermented aloe saponaria,

The present invention relates to a method for breeding pigs using a feed containing an ingredient effective in fermenting aloe saponaria.

There are about 500 kinds of aloe in the world. Six to seven of them are used as medicines. However, aloe vera, aloe arborescence, aloe saponaria, etc. are used for edible and skin- There are three kinds.

Among them, aloe saponaria has a saponin component that inhibits cancer as in ginseng and is called saponaria. The state of origin is distributed throughout Cape State, South Africa, and eastern Trans-Oval, and is used as a civilian medicine in Hawaii, Florida and other states. Natural aloe is medium-sized, has a yellow pattern on the leaves, has very short stem, grows about 50cm long and grows fast. The green leaves hang down and a long peduncle comes up from the center of the stem, and a red-orange flower blooms. If it does not have a speckle pattern, it is similar to agave, but its color is lighter and softer. Like aloe vera, it has a lot of jelly quality, it has less weakness than other aloe, it has the advantage of being the most suitable beauty material because it causes the least allergy, and it is cultivated for ornamental use.

Aloe is known to have wound healing and skin regeneration effect, skin moisturizing and anti-aging, smooth bowel activity, immunity enhancement, digestive function improvement, antibacterial and anti-inflammatory action, blood circulation promotion and anti-cancer action.

On the other hand, the Monascus family belongs to the genus Moncacaceae and exhibits different biological activities depending on the species. Red yeast rice (red koji), which has been used in many parts of China and East Asia for a long time, has been utilized as a natural food coloring agent, processed food, digestion promotion and blood flow improvement. Recently, many studies on the production and validation of natural statin (monacolin K, mevinolin, lovastatin), a substance inhibiting cholesterol biosynthesis enzyme (HMG-CoA reductase) do.

In recent years, various functions such as anticancer activity, blood pressure lowering and vasodilation effects have been scientifically proved, and saponin and isoflavones are also known to be contained in Hongkuk. Isoflavones have the effect of delaying bone loss in postmenopausal women with reduced estrogen levels.

Korean Patent Application No. 1020150098886 discloses a technique in which a probiotic bacterium is mixed with an aloe extract obtained by decompression extraction of crushed aloe, and the stirred mixture is used as a pig feed additive. However, There were no cases in which fermented soybeans were used as an animal feed additive.

Korean Patent Application No. 1020150073664 Korean Patent Application No. 1020150098886

It is an object of the present invention to provide a method for breeding pigs using a feed supplemented with fermented aloe saponaria.

The aloe saponaria fermented product to be added to the feed for pigs of the present invention is obtained by collecting raw aloe saponaria, washing it in water, pulverizing it, adding sugar to the prepared material at a weight ratio of 1: 1 and mixing , Aged at a temperature of 25-30 ° C for 6 months and aged. To the aged sugar solution was added 4 to 6 times the volume of water, adjusted to pH 4.0 by the addition of acetic acid, and then incubated at about 121 ° C for 15 minutes 5% by weight of the suspension of P. gonorrhoeae was sterilized in a sterilized diluted saline solution, and aerobic fermentation was carried out for 10 days under the temperature condition of 25-30 DEG C to obtain aloe saponaria primary fermentation product. Then, 100kg of the primary fermentation product was mixed with about 1.5 Ton, about 1.5 ton of wheat, about 2 ton of crude aloe saponaria crush stock solution and about 200 kg of zeolite to prepare a feed composition for pigs. Then, the composition for feed composition is mixed with the above-mentioned aloe soponaria primary fermentation product 5% to inoculate the 10 days aerobic fermentation aloe sand paper or Ria second fermented product prepared by the conditions at a temperature of 5-30 ℃.

As a result of analysis of the feed for lactating diets supplemented with the aloe saponaria secondary fermented product, it was confirmed to be 1.13x10 7 CFU / g of lactic acid bacteria, 7.35 × 10 6 of lactic acid bacteria, and 9.25 × 10 6 of yeast, and 2.05 × 10 7 CFU / g of Bacillus subtilis, Lactic acid bacteria 6.00x106, and yeast 7.55x106. These effective fungi inhibit diarrhea by suppressing S. typhimurium KCTC 1926, E. coli O-157: H7, Staphylococcus aueus, and Listeria, And the effect of weight gain of pigs can be expected.

The method for breeding pigs according to the present invention is characterized in that the aloe saponaria secondary fermentation product is added to the feed mixture in an amount of 3-5% by weight in the case of infant feed and 4-6% by weight in the case of infant feed, based on the total weight of the conventional pig feed The total feed intake was adjusted so that the fermented product of the present invention was administered on an average of 100-200 g / day per pig of the present invention as a feed for infants, and the growth rate of the fermented product of the present invention was 400-500 g / day Lt; RTI ID = 0.0 > of the < / RTI > total feed intake.

According to the breeding method of the present invention, there was no significant difference in the amount of feed intake compared to the conventional feed, but the amount of increase in body weight was remarkably increased with the passage of the breeding period. That is, according to the breeding method of the present invention, the weight increase amount was higher than the feeding amount, and the feed efficiency was remarkably higher than that of the general feeding method.

The increase in feed efficiency was due to the presence of S. typhimurium KCTC 1926, E. coli O-157: H7, which is a causative organism of livestock diarrhea, in feed pigments fed diets supplemented with aloe saponaria secondary fermentation ), Staphylococcus aureus, and Listeria.

The results of confirming the properties of the seedlings raised according to the present invention were shown in the following table.

Item Measure pH 5.33 ± 1.73 Water holding capacity (WHC) 76.06 ± 9.85 (%) Shear value (measured at 3-5 DEG C) 1.35 + - 0.12 (kgf) Crude fat (measured at 3-5 ° C) 3.20 ± 0.84 (%)

According to the breeding method of the present invention, it is possible to raise the efficiency of the pigs by increasing the efficiency of the pigs, and the immune system of the pigs is strengthened by the useful microorganisms contained in the feeds. Thus, antibiotics and diarrhea Use can be reduced, and the shipment weight can be increased to provide pork of high commercial quality.

The present invention will be described in detail with reference to examples. However, the following examples are illustrative of the present invention, and the present invention is not limited by the following examples.

Example : Pig breeding

 The pigs were divided into two groups. One group was fed with the control diet C (control group C) and the other group (control group T) supplemented with the second fermented aloe saponaria. Body weight and feed intake were measured once every 20 days until the 152th day.

Experimental Example 1 : Qualitative Analysis of Pork

After the end of the fattening period, the fillet muscle was rapidly picked from the boiled pears and stored at the refrigeration temperature (4 ± 1 ℃) to measure pH, water retention, shear value and crude fat content. The results are shown in Table 1.

The analytical method for each property was as follows.

1) pH

10 g of crushed pork samples were homogenized with 90 ml of distilled water using a homogenizer at 14,000 rpm for 2 minutes and measured with a pH meter (Seven Easy pH, Mettler-toledo, Switzerland).

2) Conservativeness

Centrifuge tubes were placed in a heating bath (B-490), heated at 70 ° C for 30 minutes, allowed to cool for 10 minutes, centrifuged at 100 rpm for 10 minutes, and centrifuged (Centrifuge Union 5KR), the amount of moisture measured is called F (%), the sample is divided into the same number of times and size, and then dried at 100 to 102 ° C (forced convection drying oven C-DF) The amount of water obtained by measuring the weight after cooling was calculated as W (%).

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3) Year (shear value)

Prepare the refrigerated meat product in the size of 1 cm x 3, place it on a rack of an Instron universal testing machine-tt42R (Instron, USA), zero the extension and load of the tensile tester, And the force or energy value including the interaction of the two. The shear value was measured using Warner-Bratzler at a test speed of 100 mm / min.

4) crude fat

150 ml of Folch solution (methanol: chloroform = 2: 1) was added to 10 g of sample, homogenized with a homogenizer at 14,000 rpm for 2 minutes, filtered with a Whatman No. 1 filter, and the residue and filter paper were removed from the funnel Folch solution and then filtered again. After filtration, 50 ml of distilled water was added to the filtrate. After shaking, 200 ml of the lower layer was transferred to the filtrate for 2 hours, and then chloroform was added to the filtrate for 12 hours And the extract was taken out to room temperature. The water layer was removed with a Pasteur pipette and then filled with chloroform. Then, the extract was transferred to an Erlenmeyer flask, and anhydrous sodium sulfate was sufficiently added to remove residual water. After transferring the chloroform extract to the tube, mark the amount of the extract on the outside of the tube, select a clean 50 mL beaker, display the sample name, dry it in a 100 ° C drying oven for about 1 hour, put it in a desiccator, After measuring the weight using a precision scale up to 5 decimals, record it, put 10 ml of fat extract, cover it with cheese netting, place it in a tray, blow off the solvent with the hood turned on and put the sample beaker in a 100 ° C drier, After drying for about half an hour, it was allowed to stand in a decigator for about one hour. Weights were measured to 5 decimal places using a precision scale.

A: Weight (a) of fat in 10 ml of chloroform extract, beaker weight before drying, minus beaker weight after drying

B: The amount of fat extract (200 ml)

C: First meat sample weight (10g)

D: Fat extract (10 ml)

Experimental Example  2: Weight gain and feed efficiency

(T), which is a combination of the general feed (C) and the aloe supplement, is selected and divided into 10 days until 70 days have elapsed, 20 days until 152 days elapse And the amount of daily gain was obtained by dividing the difference amount by the number of days, and it is shown in the following table. In the control group (C), high weight gain was observed at 0-10 days, 40-60 days and 130-142 days. In the experimental group (T), the highest weight gain at 0-10 days, 40-60 days, 110-130 days Respectively. There was no significant difference in daily weight gain between the control and experimental groups during each feeding period.

Also, once every 10 days until 70 days have elapsed and once every 20 days until 152 days have elapsed. The body weight changes of the pigs fed with and without aloe were shown in Table 3 below. The control and experimental groups showed a tendency to increase gradually with the lapse of time, while the experimental group showed a significant increase with the lapse of rearing period (p <0.05).

Feed amounts of the breeding pigs in the breeding process are shown in the following table.

Based on the data in Table 3 and Table 4, the feed efficiency was measured by dividing the amount of gain during a certain period by the amount of feed (Table 5).

As shown in Table 5, feed efficiency was significantly higher in the experimental group than in the control group.

Claims (2)

The raw aloe saponaria was collected, washed in water and pulverized. The prepared material was added with sugar at a weight ratio of 1: 1 and mixed. The mixture was allowed to stand for 6 months at a temperature of 25-30 캜, aged, 4.5 to 5.5 times as much volume of water was added to the saline solution, acetic acid was added thereto to adjust the pH to 4.0, and then the suspension was inoculated at 5% by weight on a diluted saline solution sterilized at 121 占 폚 for 15 minutes at 25-30 The fermented product was fermented aerobically for 10 days under the temperature condition to obtain aloe saponaria primary fermented product. To the 100 kg of the primary fermented product, 1.5 ton of octane, 1.5 ton of wheat, 2 ton of raw aloe saponaria crushed raw material and 200 kg of zeolite were added The composition for pig feed is prepared by mixing, and the composition for feed is prepared by inoculating 5% by weight of the aloe soponaria primary fermentation product into the prepared feed composition and fermenting aerobically for 10 days at a temperature of 5-30 ° C The feed mixture in which the fermented aloe saponaria fermented product is added in an amount of 3-5% by weight based on the total weight of the pig feed, 4-5% by weight in the case of feedstuff feed, The fermented product is administered in an amount of 100-200 g / day on average per pig, and the growth feed is determined by feeding the fermented product of the present invention in an amount of 400-500 g / A pig breeding method characterized by.


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