KR100943017B1 - Feed additive composition for immunopotentiating comprising extract of red-koji fermented red ginseng and stock feed comprising the same - Google Patents

Abstract

PURPOSE: A feed additive for immunopotentiation containing fermented ginseng extract is provided to ensure excellent immunological activity for improving lymphoid organ atrophy, lymphocyte reduction and immunologically active cytokine reduction caused by immunosuppressant. CONSTITUTION: A feed additive for immunopotentiation containing fermented ginseng extract is prepared by mixing sugar-soaked red ginseng with cereal powder and inoculating Monascus sp. strain; culturing the inoculated material at an initial acidity of pH 4-5.5 for 30 - 35°C for 3 days and then culturing it at 25 - 29°C for 7-37 days. The Monascus sp. Strain is one kind selected from the group consisting of Monascus rubber, Monascus purpureus Went, Monascus rubervan Tieghem, Monascus fuliginosus Sato, Monascus pilosus, Monascus pilosus Sato, Monascus anka, Monascus purpurens, Monascus albidus Sato and their mixture.

Description

Feed additive for immunity enhancement containing red ginseng fermented red ginseng extract and livestock feed containing the same {FEED ADDITIVE COMPOSITION FOR IMMUNOPOTENTIATING COMPRISING EXTRACT OF RED-KOJI FERMENTED RED GINSENG AND STOCK FEED COMPRISING THE SAME}

The present invention is Monascus sp. Fermented It relates to an immune enhancing feed additive comprising an extract of red ginseng fermented red ginseng and animal feed comprising the same.

In general, red ginseng is produced through steaming, drying, and the like after three ginseng peeling ginseng, and the browning reaction is promoted to have a dark brown color.

The red ginseng produces a special component that is beneficial to the human body due to the partial structural change of components due to pyrolysis in the manufacturing process, which is contained only in red ginseng, not in other ginsengs such as ginseng or white ginseng. It contains special ingredients such as ingredients, anti-tumor ingredients, cancer cell metastasis suppressing ingredients, antibacterial ingredients, blood sugar enhancing ingredients, anti-obesity ingredients, anti-aging ingredients and heavy metal harmful toxins.

In addition, red ginseng is more easily digested and absorbed than ginseng because starch particles in ginseng tissues are converted from a sol state to a gel state. There is a reticulum, red ginseng is processed without peeling the peel (reticulum tissue) in which such an active ingredient is accumulated, so the content of the active ingredient is high and the efficacy is excellent.

Monascus sp. Is a koji made by growing fungi of Monascus in white rice. It has been used for traditional food for a long time.

Since ancient times, Hongguk bacteria have been used in various kinds of fermented foods. In particular, red ginseng has been used for coloring and preservation purposes in alcohol, red milk curd and vegetables. In Japan, the safety of some tar-based synthetic coloring materials, which have been used as food additives since the 1970s, has become a problem, and the consumption of red rice is used as a natural pigment. In particular, hongkukyun Monacholine K has excellent effects on the prevention and treatment of chronic diseases such as hyperlipidemia, cardiovascular disease, cerebrovascular disease, arteriosclerosis, hypertension, hypotension, angina pectoris, stroke, diabetes, fatty liver, obesity, hyperglycemia, and lowering blood cholesterol In addition, there is a large amount of active substances having a similar structure and similar bioactive effects. In addition, the pigment component produced by the red yeast bacteria has anti-cancer, antibacterial and antioxidant effects. The natural pigments, functional food ingredients, such as ham and sausage meat products, miso and red pepper paste as well as vinegar, tofu, soy sauce, drinks and bread are used in various ways.

On the other hand, livestock such as pigs, cows, chickens, etc., which are used as an animal food source for humans, are bred for breeding. In raising livestock, the biggest problem is that immunity and biological activity against diseases and other environmental factors are lowered due to changes in the breeding environment. It is known that the main causes are high density breeding, increased use of chemical treatment agent, and increased feed amount of processed feed rather than natural habitat.

When the resistance to livestock disease, that is, immunity and physiological activity, is lowered, it leads to stagnation of growth and mortality.In order to prevent this, increasing the amount of antibiotics, etc., causes the accumulation of antibiotics and other drugs in vivo. As a result, it has a significant impact on the productivity and economic viability of livestock farmers.

Accordingly, there is a need for the development of feed additives that can prevent the harmful effects of drugs, such as antibiotics, and to increase the immunity of livestock by replacing antibiotics.

Therefore, the present inventors have conducted various studies on natural materials derived from natural materials to ensure safety and enhance the immunity of livestock. As a result, fermented red ginseng with Monascus sp. After confirming this, the present invention was completed.

It is an object of the present invention to provide an immune enhancing feed additive which can reduce the use of various antibiotics when raising livestock as the anti-hysteresis increases as the immunity of the livestock is enhanced.

It is another object of the present invention to provide a livestock feed comprising the feed additive for enhancing immunity.

In order to achieve the above object, the present invention is red ginseng as Monascus sp. Fermented Provided is an immune enhancing feed additive comprising an extract of red yeast fermented red ginseng and a livestock feed comprising the same.

The feed additive for immune enhancement according to the present invention is excellent in immuno-activating effect to improve lymphatic organ atrophy, lymphocyte reduction and immuno-activation cytokine reduction induced by immunosuppressive agents. Therefore, when feeding livestock, the history of the disease may be increased to reduce the use of antibiotics, as well as to reduce the economic losses of livestock farmers due to livestock diseases.

Hereinafter, the present invention will be described in more detail.

Immunity-enhancing feed additives of the present invention as red ginseng as Monascus sp. Fermented Contains red ginseng fermented red ginseng extract as an active ingredient.

At this time, any one belonging to the genus Monascus sp. Can be used, and typically, Monascus ruber , Monascus purpureus Went , Monascus rubervan tichem Tieghem , Monascus fuliginosus Sato , Monascus pilosus , Monascus pilosus Sato , Monascus anka , Monascus anus barricarium ), Monascus purpurens And Monascus albidus Sato . Preferably, a mixed strain of Monascus ruber , Monascus purpurens , Monascus anka and Monascus barykery is used.

The method of fermenting red ginseng with Monascus sp. Is not limited in the present invention, and a fermentation method known in the art is used.

According to a preferred embodiment of the present invention, red ginseng fermented red ginseng is obtained by fermenting red ginseng as follows.

First, red ginseng is washed by steaming four or more times and freeze-dried four or more times to prepare red ginseng having a moisture content of 15% or less.

Then, the red ginseng is immersed in the sugar.

Subsequently, the acupuncture red ginseng is placed on a rice tray and dried.

Thereafter, the grains pulverized about 100 ~ 150mesh is mixed and mixed by 5 to 20% (w / w) of the weight of the red ginseng is removed. At this time, the grain flour is used rice flour, barley flour, brown rice flour, soy flour or a mixture thereof, preferably brown rice flour.

Next, put the mixed grain and red ginseng into a mushroom culture container and sterilize in a sterilizer.

Subsequently, the culture medium was inoculated with Monascus sp. Strain and cultured in a shaker at 30 ° C. and 150 rpm for 5 days. Then, inoculated 5 to 20% by weight of the cultured red ginseng ( Monascus sp. ) Strain to the sterilized red ginseng and incubated for 10 to 40 days at an initial pH pH 4 to 5.5 in a solid culture room. However, the culture is maintained at 30 ~ 35 ℃ for 3 days, after which the culture is lowered to 25 ~ 29 ℃, and light-treated with an incandescent lamp and solid culture.

Finally, fermented red yeast fermented red ginseng is extracted according to the conventional extraction method of red ginseng. For example, in a red ginseng fermented red ginseng, a polar solvent such as a lower alcohol having 1 to 4 carbon atoms, preferably 5 to 10 times in water, heated at 80 to 100 ° C, preferably 80 ° C, and then heated extract After repeated extraction 1 to 10 times, filtered under reduced pressure and the filtered extracts were mixed and concentrated under reduced pressure at 20 to 100 ℃, preferably 50 to 70 ℃ with a rotary vacuum concentrator to remove the solvent.

Since the immune activity effect of the red ginseng extract is well known, in Experimental Example 1 of the present invention, in order to compare and observe the immune activity effect of the red ginseng fermented red ginseng extract, the red ginseng extract or the red ginseng fermented red ginseng extract cyclophosphamide (cyclophosphomide) , Oral oral administration to immunosuppressed mice induced by 'CPA') at 12-hour intervals, followed by body weight, thymus, swollen node and spleen weight, hematologic changes, intra-splenic TNF-α, IL-1β and IL- 10, blood IFN-γ content, and histopathological changes of spleen, knee and lymph nodes and thymus were evaluated.

 According to Experimental Example 1 of the present invention, in the CPA control group, a significant decrease in thymus, swollen node, and spleen weight, total number of blood leukocytes, red blood cells and platelets, decrease in spleen cytokine content, and blood IFN-γ content were compared to the normal control group. Although typical immunosuppressive findings such as decrease in spleen, spleen, lymph node and thymus atrophy were recognized, these CPA-induced immunosuppressive findings were marked by 250 mg / kg red ginseng extract or 500, 250 and 125 mg / kg red ginseng fermented red ginseng extract. In particular, the red ginseng fermented red ginseng extract was found to be significantly better than the red ginseng extract treated group at the same dose, and the red ginseng fermented red ginseng extract at 125 mg / kg showed a better immune activity than the 250 mg / kg red ginseng extract. Admitted.

In addition, there was no significant change in body weight except for a slight decrease in body weight by CPA administration, but as previously reported (Miyauchi et al., Tohoku J Exp Med. 1990; 162: 147-67) Significant reductions in thymus, knee and lymph nodes and spleen weight were noted. On the other hand, administration of three doses of red ginseng fermented red ginseng extract significantly inhibited the decrease in the weight of the thymus, knee, lymph nodes and spleen. It was recognized in the administration group. These results are considered to be direct evidence that the immunological activity of red ginseng is significantly increased by fermented red yeast rice.

CPA is a typical immunosuppressive agent known to cause a decrease in blood white blood cells, red blood cells and platelets by hematopoietic system inhibition. According to Experimental Example 1 of the present invention, the effect of inhibiting leukocyte reduction by CPA, which was superior to that of the red ginseng extract administration group of the same dose, was recognized in the red ginseng fermentation red ginseng extract administration group, so that the immune activity effect of red ginseng was significantly increased by the red ginseng fermentation. Judging. On the other hand, both red ginseng fermented red ginseng extract and red ginseng extract did not significantly affect the red blood cell reduction by CPA.

TNF-α is a representative cytokine produced by several types of cells including splenocytes and is known to play an important role in the differentiation of T-lymphocytes. TNF-α generally activates cellular immunity and increases the function of IL-2, which is known to promote antibody production. CPA is a typical immunosuppressive agent, which significantly reduces the number of T lymphocytes and inhibits the secretion of cytokine. IL-1 is another type of cytokine secreted from a variety of cells, including macrophages, dendritic cells, lymphocytes, endothelial cells, fibroblasts and keratinocytes. Kinds exist and are known to play an important role in the immune response. IL-10 is a typical immunosuppressive cytokine secreted by Th2 cells, some B lymphocytes and activated macrophages, and is known to inhibit the function of activated macrophages, 1995]. IFN- [gamma] is secreted from CD8 + T lymphocytes, Th1 cells and natural killer (NK) cells, affects the function of B and T cells and enhances the function of NK cells and macrophages.

Referring to Experimental Example 1 of the present invention, a decrease in the spleen content of the immunoactive cytokines TNF-α, IL-1β and the blood content of IFN-γ was observed by CPA administration, and immunity according to T lymphocytes and immunosuppression A splenic decrease in the inhibitory cytokine IL-10 was also recognized. On the other hand, compared to the red ginseng extract administration group of the same dose, the effect of inhibiting the reduction of the spleen and blood cytokine content by CPA was recognized in the red ginseng fermented red ginseng extract administration group.

CPA results in a significant decrease in T lymphocytes, resulting in significant atrophy of the spleen, knee and lymph nodes, and thyroid gland. In Experimental Example 1 of the present invention, the administration of CPA resulted in significant atrophy due to the reduction of lymphocytes in the thymus, knee and lymph nodes and spleen, and thus the decrease in lymph node thickness was recognized. On the other hand, the superior effect than the inhibition of lymphatic organ atrophy or lymphocyte reduction by administration of the same dose of red ginseng extract was recognized in the red yeast fermented red ginseng extract administration group, respectively. Compared with red ginseng, the immunological activity of red ginseng fermented red ginseng extract was observed in the immunosuppressive mice induced with CPA. Red ginseng fermented red ginseng extract 125mg / kg administration group was confirmed to have a better immune activity than 250mg / kg red ginseng extract administration group. Therefore, the immune activity of red ginseng is considered to be significantly increased by the fermentation of red yeast rice.

Therefore, the red yeast fermented red ginseng extract according to the present invention can be used as a feed additive for boosting immunity for feeding livestock.

In addition, the red yeast fermented red ginseng extract of the present invention can be added to the general feed can be fed to animals. In this case, preferably, the red yeast fermented red ginseng extract is added in an amount of 0.1 to 30% by weight. If the content of the feed additive for immune enhancing is less than the above range it is difficult to expect the effect of the addition, on the contrary, if exceeding the above range there is a risk of unbalanced nutrition of the livestock.

Since the general feed is different in its composition and manufacturing method according to the type of livestock, the composition and manufacturing method are not particularly limited in the present invention. These feeds are designed to accurately identify nutrient requirements at each growth stage and to be designed with high levels of total nutrition.

The livestock may be cattle, cows, horses, donkeys, pigs, sheep, goats, dogs, rabbits, cats, ducks, chickens, geese, pheasants, quails, or turkeys.

Hereinafter, preferred examples and experimental examples are presented to help understand the present invention. However, the following Examples and Experimental Examples are provided only to more easily understand the present invention, and the contents of the present invention are not limited by the Examples.

Example 1: Preparation of red yeast fermented red ginseng extract

Washing the fresh ginseng 4 times and freeze-dried 4 times to prepare a red ginseng with less than 15% moisture content. The red ginseng thus prepared was dipped in 50% (w / v) sugar water for one hour. Subsequently, the dried red ginseng was placed on a tray, and the water was removed for 6 hours, and the brown rice powder pulverized about 120 mesh was added and mixed with 10% (w / w) of the weight of the red ginseng.

Then, mixed brown rice and red ginseng were put into a mushroom culture container and sterilized at 121 ° C. for 20 minutes in a sterilizer. Thereafter, Monascus pilosus KCCM 60084 strain was inoculated in PD broth medium and incubated for 5 days in a shaking incubator at 30 ° C. and 150 rpm.

The cultured Monascus strain was inoculated 10% by weight in sterilized red ginseng, and then incubated in a solid culture room for 15 days at pH pH 5, and then maintained at 35 ° C. for 3 days, after which the temperature was 28 ° C. The culture was lowered and incubated with an incandescent lamp for solid culture. 2L purified water was added to 200 g of the red ginseng fermented red ginseng thus obtained, heated to 80 ° C., and the extract obtained by concentrating under reduced pressure with a rotary evaporator was lyophilized to obtain red ginseng fermented red ginseng extract powder.

Comparative Example 1: Preparation of Red Ginseng Extract

The red ginseng prepared by the conventional red ginseng manufacturing method by steaming and drying ginseng was washed thoroughly and soaked in cold water for 12 hours. 2L purified water was added to 200 g of red ginseng, heated to 80 ° C, and the extract obtained by concentrating under reduced pressure with a rotary evaporator was lyophilized to obtain red ginseng extract powder.

Experimental Example 1: Confirmation of the immunological activity of red ginseng fermented red ginseng extract

In order to confirm the immunological activity of the red yeast fermented red ginseng extract according to the present invention, the following experiment was performed.

Six-week-old ICR mice (SLC, Japan) were used as experimental animals after 7 days of adaptation time, and were reared in a polycarbonate breeding box for every five animals. Nine animals were used for each experimental group, and breeding conditions were 20 to 25 ° C., 40 to 45% humidity, and 12 hours / day fluorescent lighting. During the acclimation period, water and feed (Samyang, KR) were fed freely.

In order to observe the immune activity effect of the red ginseng fermented red ginseng extract compared with the red ginseng extract, the red ginseng prepared in Example 1 orally four times at 12 hour intervals in immunosuppressed mice induced with cyclophosphomide (CPA) Fermented red ginseng extract powder and the red ginseng extract powder prepared in Comparative Example 1 were administered, respectively, and then the weight, thymus, swollen node and spleen weight, hematological changes, intratumoral necrosis factor-α (TNF) -α, interleukin- The histopathological changes of 1β (IL-1β) and interleukin-10 (IL-10) content, blood interferon-γ (IFN-γ) content, spleen, knee and lymph nodes, and thyroid gland were evaluated. The test materials were suspended in sterile distilled water and administered at 10 ml / kg, respectively.

Immunosuppression was induced by disintegrating 150 mg / kg and 110 mg / kg of CPA in physiological saline two and three times per day at a concentration of 10 ml / kg 3 and 1 day prior to the start of administration of the test substance. In the negative control, the same amount of physiological saline was administered in the same manner instead of CPA. The specific experimental design is shown in Table 1 below.

division Dose (mg / kg / day) process Substance Normal control 10 ml / kg - Distilled water CPA control 10 ml / kg CPA Distilled water Positive control 250 mg / kg CPA Red Ginseng Extract Experimental group 1 500 mg / kg CPA Red Ginseng Fermented Red Ginseng Extract Experiment group 2 250 mg / kg CPA Red Ginseng Fermented Red Ginseng Extract Experiment group 3 125mg / kg CPA Red Ginseng Fermented Red Ginseng Extract

1-1. Change in weight

Body weight change was measured using an electronic scale (Precisa Instrument, Switzland) one day before the first CPA, the first CPA, the second CPA, before the test substance, one day after administration, and at the time of sacrifice. And the results are shown in Figure 1 below.

Referring to FIG. 1, a significant decrease in body weight was observed from the start date of administration in all CPA treated groups compared to the normal control group, but no significant change in body weight was recognized in all administered groups compared to the CPA control group.

1-2. Change in organ weight

At the sacrifice of experimental animals, the weights of the thymus, left knee, lymph nodes and spleen were measured using an electronic scale (Precisa Instrument, Switzland), and the relative weight of organs to body weight was measured to reduce the difference in weight. It is shown in Table 2 below.

Referring to Table 2, the CPA control group showed a significant (p <0.01 or p <0.05) reduction in the weight of the thymus, knee, lymph nodes and spleen, compared to the normal control group, but significantly (p <0.01 or p). <0.05) Thyroid gland, knee, lymph node and spleen weight gains were recognized in all three doses of red ginseng fermented red ginseng extract group.

On the other hand, the red ginseng extract administration group showed significant increase in relative (p <0.01 or p <0.05) thyroid gland relative weight and absolute weight, and absolute value of knee and lymph nodes and spleen and lymph nodes, respectively. Were each recognized. Compared with the same dose of red ginseng extract, the effect of significantly suppressing the immune organ weight loss was recognized in the red ginseng fermented red ginseng extract group, and the better than the 250 mg / kg red ginseng extract group in the red ginseng fermented red ginseng extract group. The effect was recognized.

The thymus gland weight was -74.61% in the CPA control group compared to the normal control group, and 45.07, 66.32, 68.48 and 64.60% in the red ginseng and red ginseng fermented red ginseng extract 500, 250 and 125 mg / kg groups. Each change is shown.

The relative weight of the spleen was -59.75% in the CPA control group compared to the normal control group, and 19.02, 26.10, 26.53 and 25.34% of the red ginseng extract, red yeast fermented red ginseng extract 500, 250 and 125 mg / kg compared to the CPA control group. Each change is shown.

The relative weights of the knee and lymph nodes were -33.16% in the CPA control group compared to the normal control group, and the red ginseng extract, red ginseng fermented red ginseng extract 500, 250 and 125 mg / kg were 33.78, 66.24, 75.23 and 97.99% change was shown respectively.

1-3. Hematological changes

Blood was collected from the vena cava at the time of sacrifice in experimental animals, and the total blood leukocyte count was measured using an automatic blood cell counter (MS9-5V; Melet Schloesing Lab., France), and the results are shown in Tables 3 and 4 below. .

Referring to Tables 3 and 4 above, a significant (p <0.01) decrease in leukocyte count, erythrocyte count, hematocrit, hemoglobin concentration and platelet count was found in the CPA control group compared to the normal control group. Was admitted.

On the other hand, the decrease in blood leukocyte count was significantly (p <0.01 or p <0.05) inhibited by administration of three doses of red ginseng fermented red ginseng and red ginseng, but the erythrocyte count, hematocrit, hemoglobin concentration level and platelet count were similar to those of CPA control group. Was observed. Compared with the same dose of red ginseng extract administration group, the remarkably superior effect of reducing blood white blood cell count was recognized in the fermented red ginseng extract administration group.

The total leukocyte counts in blood were -92.40% in the CPA control group compared to the normal control group, and the red ginseng extract, red ginseng fermented red ginseng extract 500, 250 and 125 mg / kg were 61.09, 90.27, 88.72 and 92.22 compared to the CPA control group. The change in% is shown respectively.

The blood red blood cell count was -16.01% in the CPA control group compared to the normal control group, and the red ginseng extract, red ginseng fermented red ginseng extract 500, 250 and 125 mg / kg group showed 2.20, -0.73, 2.32 and- Changes of 1.43% were shown respectively.

Hematocrit was -14.99% change in the CPA control group compared to the normal control group, and changes of 6.01, 4.66, 7.99 and 1.71% in the red ginseng extract, red ginseng fermented red ginseng extract 500, 250 and 125 mg / kg group compared to the CPA control group. Are shown respectively.

The hemoglobin concentration was -40.92% in the CPA control group compared to the normal control group, and the red ginseng extract, red ginseng fermented red ginseng extract 500, 250 and 125 mg / kg were 1.09, 0.17, 1.76 and -2.09% compared to the CPA control group. The change of is shown, respectively.

The platelet count of the CPA control group was -81.11% compared to the normal control group, and the red ginseng extract, fermented red ginseng extract 500, 250 and 125 mg / kg showed 5.10, 8.75, 1.71 and 1.18% of the CPA control group. Each change is shown.

1-4. Changes in Serum IFN-γ Content

Blood was collected and serum was prepared in the same manner as in the above 1-3. Prepared serum samples were analyzed using mouse TNF-α Elisa kit (BD Biosciences / Pharmingen, CA, USA), and the results are shown in FIG. 2.

Referring to FIG. 2, a significant (p <0.01) decrease in serum IFN-γ content was observed in the CPA control group compared to the normal control group, but in the three doses of the red yeast fermented red ginseng and red ginseng extract administration groups, it was significantly (p <0.01 or p <0.05) Significant increases in blood IFN- [gamma] content were recognized, respectively. On the other hand, compared to the red ginseng extract administration group of the same dose, significantly lower inhibitory effect of IFN-γ content in blood was recognized in the red yeast fermented red ginseng administration group.

The serum IFN-γ content was -71.12% in the CPA control group compared to the normal control group, and the red ginseng extract, red ginseng fermented red ginseng extract 500, 250 and 125 mg / kg were 35.45, 59.90, 62.60 and 49.55 compared to the CPA control group. The change in% is shown respectively.

1-5. Changes in Spleen Cytokine Content

TNF-α, IL-1β and IL-10 contents of the spleen were measured by mouse TNF-α Elisa kit (BD Biosciences / Pharmingen, CA, USA), mouse IL-1β Elisa kit (Genzyme, MA, USA) and Mouse IL, respectively. Measured using -10 Elisa kit.

The change in the content of the spleen cytokine measured in this way is shown in Table 5 below.

Referring to Table 5, a significant (p <0.01) decrease in splenic TNF-α, IL-1β and IL-10 contents was recognized in the CPA control group compared to the normal control group, but three doses of red yeast fermented red ginseng extract and red ginseng extract Significant (p <0.01 or p <0.05) increase in TNF-α, IL-1β and IL-10 contents in the spleen compared to the CPA control group was observed.

On the other hand, compared to the red ginseng extract administration group of the same dose, the effect of inhibiting the reduction of IFN-γ content in blood was recognized in the red ginseng fermented red ginseng group, and the red ginseng fermented red ginseng extract 125 mg / kg group showed better suppression than the 250 mg / kg red ginseng extract group. The effect was recognized.

The TNF-α content in the spleen blood was -53.74% in the CPA control group compared to the normal control group, and the red ginseng extract, red ginseng fermented red ginseng extract 500, 250 and 125 mg / kg were 23.52, 59.76, 51.61 and Changes of 42.76% were shown, respectively.

The IL-1β content in the spleen blood was -67.33% in the CPA control group compared to the normal control group, and the red ginseng extract, red ginseng fermented red ginseng extract 500, 250 and 125 mg / kg were 39.16, 65.09, 53.70 and 52.54% change was shown respectively.

The IL-10 content in the spleen blood was -57.40% in the CPA control group compared to the normal control group, and the red ginseng extract, red ginseng fermented red ginseng extract 500, 250 and 125 mg / kg were 33.75, 78.65, 56.02 and 50.13% change was shown respectively.

1-6. Histopathological changes in the thymus

Using the spleen of each test group as a sample, the tissues were fixed with 10% neutral buffered formalin, embedded in paraffin, and then prepared 3-4 μm sections. Representative sections were stained with H & E for each test group, observed with an optical electron microscope, and the total thickness and cortical thickness were measured by an automatic DMI CCD image analyzer (DMI, KR).

3 is a normal control group (A, B), CPA control group (C, D), positive control group (E, F), experimental group 1 (G, H), experimental group 2 (I, J) and The thorax gland of experimental group 3 (K, L) was photographed by H & E staining and observed by optical electron microscope (scale bar = 80 μm).

As shown in Table 6 and FIG. 3, the CPA control group showed atrophy characterized by a significant decrease in lymphocytes in the thymus cortex compared to the normal control group, and showed significant (p <0.01) thyroid gland and cortical thicknesses compared to the normal control group. Reductions were acknowledged respectively.

Meanwhile, administration of three doses of red ginseng fermented red ginseng extract significantly inhibited atrophy of the thymus, indicating a significant increase (p <0.01 or p <0.05) in the thickness of the entire thymus and cortex compared to the CPA control. In addition, the red ginseng extract 250mg / kg group showed a significant (p <0.05) increase in thyroid cortex thickness and a significant increase in total thymus gland thickness compared to the CPA control group. Therefore, the atrophy inhibiting effect of the thymus gland was remarkably superior to that of the red ginseng fermented red ginseng extract group compared with the same dose of the red ginseng extract group.

The total thickness of the thymus gland was -54.04% in the CPA control group compared to the normal control group, and 21.09, 41.65, 36.11 and 32.73% in the red ginseng extract, red yeast fermented red ginseng extract 500, 250 and 125 mg / kg group compared to the CPA control group. Each change is shown.

The thymus cortex thickness was -65.25% in the CPA control group compared to the normal control group, and the red ginseng extract, red ginseng fermented red ginseng extract 500, 250 and 125 mg / kg were 26.10, 60.14, 57.36 and 44.98% of the CPA control group. Each change is shown.

1-7. Histopathological changes in the spleen

The histopathology of the spleen was observed in the same manner as in 1-6, and the results are shown in Table 7 and FIG. 4.

4 is a normal control group (A, B), CPA control group (C, D), positive control group (E, F), experimental group 1 (G, H), experimental group 2 (I, J) and Spleens of Experimental Group 3 (K, L) were photographed by H & E staining and observed with an optical electron microscope (scale bar = 80 µm).

As shown in Table 7 and FIG. 4, in the CPA control group, atrophy is characterized by a significant decrease in lymphocytes in the splenic white membranous region as compared to the normal control group, and has a significant (p <0.01) spleen thickness and white color than the normal control group. Reductions in water diameter and number were recognized, respectively. Meanwhile, administration of three doses of red yeast fermented red ginseng extract or red ginseng extract significantly inhibited the spleen atrophy, and the spleen thickness, the number and diameter of the white medulla were significantly increased compared to the CPA control group. Red ginseng fermented red ginseng extract 125mg / kg administration group was confirmed to have a better spleen atrophy inhibitory effect than 250mg / kg red ginseng extract administration group.

The total thickness of the spleen was -36.29% in the CPA control group compared with the normal control group, and 19.80, 28.60, 25.16, and 23.11% of the red ginseng extract, red yeast fermented red ginseng extract 500, 250, and 125 mg / kg were compared with the CPA control group. Each change is shown.

The number of splenic white medulla was -55.74% in the CPA control group compared to the normal control group, and the red ginseng extract, red ginseng fermented red ginseng extract 500, 250 and 125 mg / kg were 24.07, 88.89, 62.96 and 51.85 compared to the CPA control group. The change in% is shown respectively.

The diameter of splenic white medulla was -32.19% in the CPA control group compared to the normal control group, and 16.77, 29.94, 29.55 and 28.08 in the red ginseng extract, red ginseng fermented red ginseng extract 500, 250 and 125 mg / kg group compared to the CPA control group. The change in% is shown respectively.

1-8. Histopathological Changes in the Hip and Lymph Nodes

The histopathology of the knee and lymph nodes was observed in the same manner as in 1-6, and the results are shown in Table 8 and FIG. 5.

5 is a normal control group (A, B), CPA control group (C, D), positive control group (E, F), experimental group 1 (G, H), experimental group 2 (I, J) and Sl and lymph nodes of Experimental Group 3 (K, L) were photographed by H & E staining with an optical electron microscope (scalebar = 80 μm).

Referring to Table 8 and FIG. 5, the atrophy is characterized by a significant lymphocyte reduction in the part of the knee and lymph node cortex in the CPA control group compared to the normal control group, and significantly (p <0.01) total thickness and follicle of the knee and lymph node compared to the normal control group. Reductions in diameter were recognized respectively.

On the other hand, administration of three doses of red ginseng fermented red ginseng extract or red ginseng extract significantly inhibited lymph node reduction in lymph nodes, resulting in a significant (p <0.01 or p <0.05) increase in the total thickness of follicle lymph nodes and follicles compared to CPA controls. Indicated. As a result of this experiment, remarkably superior atrophy inhibition effect of sesame and lymph nodes was observed in the red yeast fermented red ginseng extract group.

The total thickness of the knee and lymph nodes was -49.33% in the CPA control group compared to the normal control group, and 33.72, 68.53, 68.06 and 55.95% in the red ginseng extract and red ginseng fermented red ginseng extract 500, 250 and 125 mg / kg group. The change of is shown, respectively.

The follicle lymph node diameter was -56.86% in the CPA control group compared with the normal control group, and the red ginseng extract, the red ginseng fermented red ginseng extract 500, 250 and 125mg / kg were 30.84, 77.37, 68.31 and 55.30% compared with the CPA control group. The change of is shown, respectively.

Immunity-enhancing feed additives according to the present invention can improve the immune ability of the livestock, reduce the economic damage caused by livestock diseases of the livestock farms, can improve the livestock breeding environment.

1 is a graph showing the weight change of each test group in Experimental Example 1 of the present invention.

Figure 2 is a graph showing the IFN-γ content in blood of each test group in Experimental Example 1 of the present invention.

3 is a normal control group (A, B), CPA control group (C, D), positive control group (E, F), experimental group 1 (G, H), experimental group 2 (I, J) and The thorax gland of experimental group 3 (K, L) was photographed by H & E staining and observed by optical electron microscope (scale bar = 80 μm).

4 is a normal control group (A, B), CPA control group (C, D), positive control group (E, F), experimental group 1 (G, H), experimental group 2 (I, J) and Spleens of Experimental Group 3 (K, L) were photographed by H & E staining and observed with an optical electron microscope (scalebar = 80 μm).

5 is a normal control group (A, B), CPA control group (C, D), positive control group (E, F), experimental group 1 (G, H), experimental group 2 (I, J) and Sl and lymph nodes of Experimental Group 3 (K, L) were photographed by H & E staining with an optical electron microscope (scalebar = 80 μm).

Claims (5)

Grain powder was mixed with Acupuncture red ginseng and inoculated with Monascus sp. Immunity-enhancing feed additive comprising an extract of red yeast fermented red ginseng, which is produced by culturing at a temperature. The method of claim 1, Monascus sp. Is Monascus ruber , Monascus purpereus Went , Monascus rubervan Tieghem , Monascus fuliginosus Sato , Monascus pilosus , Monascus pilosus Sato , Monascus anka , Monascus barykery , Monascus purusens , Monascus albidus Sato ( Monascus albidus Sato ) and a feed additive for immune enhancement, characterized in that one selected from the group consisting of. delete Animal feed comprising 0.1 to 30% by weight of the feed additive for immune enhancement of claim 1. The livestock feed of claim 4, wherein the livestock is cow, cow, horse, donkey, pig, sheep, goat, dog, rabbit, cat, duck, chicken, goose, pheasant, quail, or turkey.

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