KR101876566B1 - Method for manufacturing the composition of improved Lactic acid bacteria feed additive and Lactic acid bacteria manufactured by the same - Google Patents

Abstract

A method for producing a lactic acid bacterium preparation for livestock feed addition is disclosed. The method for producing a lactic acid bacterium preparation for livestock feed addition according to an embodiment of the present invention comprises the steps of: a) inoculating and culturing yeast, lactic acid bacteria and Bacillus bacteria in a culture composition obtained by mixing 2 parts by weight of a microbial culture medium with 100 parts by weight of purified water ; b) The liquid microorganisms cultured in the step a) are fixed in a solid medium composed of rice bran, zeolite and wheat bran at a temperature of 30 ° C with a moisture content of 40%, air is injected and stirred in a solid incubator for 3 days to prepare a microorganism culture step; c) stirring the microbial cultures of step b) for 3 hours with stirring, blowing the hot air generated by the electric heater with a blower and drying for 3 hours; d) pulverizing the microbial cultures dried in step c) into predetermined particles and packaging them.

Description

TECHNICAL FIELD The present invention relates to a method for producing a lactic acid bacterium for livestock feed addition and a lactic acid bacterium preparation prepared thereby,

The present invention relates to a process for producing a lactic acid bacterium preparation for livestock feed addition and a lactic acid bacterium preparation prepared thereby, and more particularly, to a lactic acid bacterium preparation capable of inhibiting the activity of a bactericidal and pathogenic bacterium by producing lactic acid and an antibacterial substance, And a lactic acid bacterial preparation produced thereby.

So far, livestock industry has rapidly become intensified, commercialized, and large-scale, and various techniques have been applied to maximize productivity. However, as the livestock facilities are modernized and livestock are kept in high density, antibiotics for growth promotion and disease prevention have been abused. When animals are kept in a confined space, they are weakened by disease and poor growth, so that they can only use antibiotics for growth promotion and disease prevention.

The use of antibiotics can be satisfactory for immediate effect, but it induces microorganisms resistant to antibiotics in the intestines of livestock, induces the problem of residual antibiotics in livestock products, And the like.

For this reason, there is a growing interest in the development of safe materials that can replace antibiotics. For example, probiotics may be used as a substitute for antibiotics. The lactic acid bacteria preparation improves the productivity by keeping the intestinal microorganism gun normally, and at the same time, the physiologically active substances such as enzymes, antimicrobial substances, .

Due to such a change of environment, the present invention was intended to develop a product that can replace antibiotics and growth promoters.

Korea Patent No. 10-0923226 Korea Patent No. 10-0410830

SUMMARY OF THE INVENTION The present invention provides a method for producing a multi-functional lactic acid bacteria preparation which can be added to an animal feed.

In order to attain the above object, the present invention provides a method for culturing a microorganism, comprising the steps of: (a) inoculating and culturing a culture composition obtained by mixing 2 parts by weight of a microbial culture medium with 100 parts by weight of purified water; b) The liquid microorganisms cultured in the step a) are sprayed to a solid medium composed of rice bran, corn flour, zeolite and wheat bran using a pump and a nozzle, and fixed at a temperature of 35 ° C and a temperature of 35 ° C. Preparing a cultured lactic acid bacterium with stirring for 60 hours; c) stirring the cultured lactic acid bacteria of step b) for 3 hours with stirring, blowing the hot air generated by the electric heater with a blower and drying for 3 hours; And d) pulverizing the cultured lactic acid bacteria cultured in the step c) into predetermined particles and packing the lactic acid bacteria cultured in the step c).

In the step a), the lactic acid bacterium is Lactobacillus plantarum and is cultured at 35 ° C for 24 hours.

In step a), the microorganism medium may be selected from the group consisting of soybean peptone, yeast extract, glucose anhydrous, sodium acetate, ammonium citrate, sodium bicarbonate, , J. potassium phosphate _{(K 2 HPO 4 (0.1%} ), the best potassium phosphate _{(KH 2 PO 4 (0.1%} )), magnesium heptahydrate (MgSO ₄ .7H ₂ O sulfate), manganese (MnSO ₄ .H ₂ O) , Zinc sulfate heptahydrate (ZnSO ₄ .7H ₂ O).

The solid medium is preferably composed of 50% by weight of rice bran, 40% by weight of corn flour, 5% by weight of zeolite and 5% by weight of wheat flour and the solid incubator is rotated at a rate of one rotation per 15 minutes for 60 hours .

The lactic acid bacteria contained in the lactic acid bacterial preparation of the present invention produce lactic acid during the cultivation process to lower the acidity (pH 4) and generate hydrogen peroxide (H2O2), thereby inhibiting the growth of pathogenic microorganisms and preventing soil disease. In addition, the production of various physiologically active substances (vitamins, amino acids, nucleic acids, etc.), antimicrobial substances and various enzymes increases the ability of plants to self-defense plants. In the case of livestock, stabilization of intestinal microorganisms, Increase effect.

The lactic acid bacterium of the present invention is Lactobacillus plantarum and is produced by culturing at 35 DEG C for 24 hours.

Zeolite, also called zeolite, is a silicate hydrate of mainly aluminum, sodium and calcium and is represented by the general formula W _m Z _n O _2n SH ₂ O. W is Na, Ca, K (Ba, Sr), Z is Si + Al, and S is not constant. Has a property that it has cation exchanged easily such as Na ⁺ (or K ⁺ ) and is substituted with Ca ^{2 +} or Mg ^{2 +} in water to soften the water.

In the present invention, the rice bran is separated when the rice is crushed, and refers to the rice husk excluding the white rice and the outer surface in the brown rice. Rice bran contains various active ingredients such as unsaturated fatty acid, protein, carbohydrate, vitamin E, dietary fiber and orizanol as a nutrient source, and it is known that rice bran has various physiological effects including suppression of cholesterol elevation.

The embodiments of the present invention help the proliferation of beneficial bacteria in the intestines and produce lactic acid and antimicrobial substances, thereby inhibiting the activities of the spoilage bacteria and pathogenic bacteria.

In addition, the palatability and flavor of the feed is enhanced, and nutrient supply improves the growth rate and feed efficiency.

In addition, the generation of hydrogen sulfide (H2S) and ammonia (NH3) gas is reduced, and the deodorizing effect of the excrement is excellent.

In addition, digestion of livestock is facilitated by the inclusion of various degrading enzymes, and the production of enzymes such as amylase, protease, and lipase accelerates the decomposition of various organic materials and the compost fermentation.

In addition, it promotes the digestion of livestock and inhibits the growth of pathogenic microorganisms, so that the growth of livestock can grow normally, and various kinds of degrading enzymes, amino acids, vitamins, nucleic acids and antimicrobial substances are produced, Thereby increasing the disease resistance and treating and preventing diseases.

FIG. 1 is a process flow diagram of manufacturing a lactic acid bacterium preparation for livestock feed according to an embodiment of the present invention.

Hereinafter, the present invention will be described in more detail with reference to Examples. It will be apparent to those skilled in the art that these embodiments are only for describing the present invention in more detail and that the scope of the present invention is not limited by these embodiments in accordance with the gist of the present invention .

Liquid seed culture

Lactic acid bacteria were inoculated into a culture composition prepared by mixing 2 parts by weight of a microorganism medium with 100 parts by weight of purified water, and cultured. The lactic acid bacteria were cultured at 35 DEG C for 24 hours using Lactobacillus plantarum.

4.5% by weight of peptone derived from soybean, 15.85% by weight of yeast extract, 56.61% by weight of anhydrous glucose, 11.32% by weight of sodium acetate, ammonium citrate, 2.26% by weight of sodium bicarbonate, 2.26% by weight of potassium phosphate (K ₂ HPO ₄ (0.1%)), 2.26% by weight of potassium phosphate monobasic (KH ₂ PO ₄ (0.1% 0.23% by weight of whiskers (MgSO ₄ .7H ₂ O), 0.02% by weight of manganese (MnSO ₄ .H ₂ O), and 0.11% by weight of zinc sulfate heptahydrate (ZnSO ₄ .7H ₂ O) was used.

There is a problem that the culture temperature is higher than this temperature range and remarkably low, and the growth rate is lowered.

The fermentation time of the fermentation is not particularly limited, but if the fermentation time is less than 24 hours, the fermentation becomes insufficient. If the fermentation time is longer than 24 hours, the fermentation time is not more effective than that of the time.

Preparation of solid culture lactic acid bacteria preparation

The liquid lactic acid bacteria cultured in step a) was sprayed to a solid fermentation medium composed of rice bran, corn flour, zeolite, and wheat bran using a pump and a nozzle, and fixed at a temperature of 35 ° C and a temperature of 35 ° C. Followed by stirring to produce a lactic acid bacterium culture.

The solid fermentation broth was mixed with 50% by weight of rice bran, 40% by weight of corn flour, 5% by weight of zeolite, and 5% by weight of wheat flour. The solid phase fermentation broth was inoculated to the solid fermentation medium to have a water content of 40%.

By controlling the water content only by the liquid seed bacterium, the process can be simplified and the solid fermentation efficiency can be increased.

When the water content is too low, the growth rate of the microorganisms is low and the culture takes a long time. On the contrary, when the water content is too high, the growth rate of the microorganisms is high, but the fungi and the like show contamination, There is a problem that the manufacturing cost in the drying process performed in the post-process increases. The inoculated solid fermentation medium was stirred at a rate of one turn per 15 minutes for 60 hours to prepare a solid culture lactic acid bacterial preparation.

Drying and crushing

It is stored in agitating and transporting machine, stirred once every 3 hours, and heat generated by electric heater is blown to dry during transferring. The electric heater was operated at 30 kW and the blower was dried at 7.5 hp for 3 hours. The dried lactic acid bacterium preparation was pulverized into certain particles to complete the product.

It was confirmed that the number of lactic acid bacteria contained in the lactic acid bacterial preparation after the completion of the lactic acid bacteria preparation contained 1.0 × 10 ⁹ cfu / kg or more of saccharomyce cerevisiae.

<Experimental Example 1> Test of growth rate of pigs after feeding the lactic acid bacterium preparation of the present invention

The pig weight gain test was performed as follows. The test animals were fed with the lactic acid bacterium of the present invention for feeding according to the present invention to piglets of up to 11 weeks old at about 4 weeks of age and fed to the control group (N = 15, 15, 16) (N = 15, 16, and 16) were 6.5 and 6.3 kg respectively. The weight of each pig was measured at 1, 2, 4, 5, 6, and 7 weeks after feeding the microbial agent. The results are shown in Table 3 and FIG.

Mean weight change of experimental group and control pig (change in weight gain) (unit: kg)
Group

Salary before
After salary
1 week 2 weeks 4 weeks 5 weeks 6 weeks 7 weeks A. Experimental group
(N = 58.3
groups)
6.5
8.8
12.0
17
22.6
27.4
32.1 B. Control group
(N = 64, 3 groups)
6.3
8.5
11.4
16.1
21.3
26.0
30.1 A-B 0.2 0.3 0.6 0.9 1.3 1.4 2.0

As a result, in the case of the experimental group, the increase rate of the average of about 1 kg was increased from the second week after the feeding of the microorganism preparation of the present invention over the entire period, and the average of 2.0 kg was increased at the 7th week after the end of the experiment Respectively.

<Experimental Example 2> Effect of the present invention on prevention of diarrhea in pigs after feeding with lactic acid bacterium preparation

In addition, diarrhea could be prevented by preventing diarrhea by 80% or more.

Prevention of diarrhea in experimental and control pigs
Group Diarrhea Prevention Effect Incidence rate Preventive effect Experimental Section 3.2% 81.7% Control 17.5% -

<Experimental Example 3> Experiments on the effects of the present invention

20,000 chickens from chicken farms in Chonbuk province were selected for about 40 days from August 31, 2016 to October 9, 2016, and the lactic acid bacterial preparation of this example was added to the feed in an amount of 0.1% I ate the chicken. The result was that the average delivery time of the control was 40 days, and it was 5 days earlier than the shipping time of the control. Therefore, the weight gain effect was about 12.5%

Blood effect of poultry Until the time of release
Days Release
Speed Out of season
weight Control 40 days - 1.7kg Experimental Section 35 days 12.5% 1.7kg

As a result of measuring the concentration of noxious gas in the intestines after feeding the lactic acid bacterium preparation of the present invention. It was confirmed that the concentration of noxious gases such as ammonia (NH 3) and hydrogen sulfide (H 2 S) generated from the starch was remarkably decreased before the lactic acid bacteria preparation was fed.

This is because not only the metabolism in the intestines of the shaft body is activated but also the activity of the bacteria continues even after the manure is excreted, thereby reducing the ammonia gas and the volatile fatty acid which are the main cause of the smell of the animal manure.

It will be apparent to those skilled in the art that various modifications and variations can be made in the present invention without departing from the spirit and scope of the invention. Accordingly, such modifications or variations are intended to fall within the scope of the appended claims.

Claims (5)

a) Peptone-derived peptone, yeast extract, glucose anhydrous, sodium acetate, ammonium citrate, sodium bicarbonate, sodium bicarbonate, J. potassium phosphate _{(K 2 HPO 4 (0.1%} ), the best potassium phosphate _{(KH 2 PO 4 (0.1%} )), magnesium sulfate heptahydrate (MgSO ₄ .7H ₂ O), manganese (MnSO ₄ .H ₂ O), Lactobacillus plantarum, which is a lactic acid bacterium, is inoculated into a culture composition prepared by mixing 2 parts by weight of a microorganism medium composed of zinc sulfate heptahydrate (ZnSO ₄ .7H ₂ O) and culturing at 35 ° C for 24 hours;
b) The liquid microorganism cultured in step a) is sprayed to a solid medium consisting of 50% by weight of rice bran, 40% by weight of corn powder, 5% by weight of zeolite and 5% by weight of wheat flour using a pump and a nozzle, Stirring the mixture at a rate of one rotation per 15 minutes for 60 hours to prepare a cultured lactic acid bacterium;
c) stirring the cultured lactic acid bacteria of step b) for 3 hours with stirring, blowing the hot air generated by the electric heater with a blower and drying for 3 hours; And
d) pulverizing the cultured lactic acid bacteria cultured in step c) into predetermined particles and packaging,
Wherein the number of lactic acid bacteria contained in the cultured lactic acid bacterium after saccharomyces cerevisiae is 1.0 x 10 &lt; ⁹ &gt; cfu / kg or more after completion of step d). delete delete delete delete

Images