KR101865870B1 - 변형된 ＲＮＡｓｅ Ｈ 및 핵산 증폭의 검출 - Google Patents

변형된 ＲＮＡｓｅ Ｈ 및 핵산 증폭의 검출 Download PDF

Info

Publication number
KR101865870B1
KR101865870B1 KR1020127001640A KR20127001640A KR101865870B1 KR 101865870 B1 KR101865870 B1 KR 101865870B1 KR 1020127001640 A KR1020127001640 A KR 1020127001640A KR 20127001640 A KR20127001640 A KR 20127001640A KR 101865870 B1 KR101865870 B1 KR 101865870B1
Authority
KR
South Korea
Prior art keywords
rnase
sequence
rna
lys
enzyme
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
KR1020127001640A
Other languages
English (en)
Korean (ko)
Other versions
KR20130021351A (ko
Inventor
윈덴 쳉
제이슨 옵디케
Original Assignee
한화에어로스페이스 주식회사
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 한화에어로스페이스 주식회사 filed Critical 한화에어로스페이스 주식회사
Publication of KR20130021351A publication Critical patent/KR20130021351A/ko
Application granted granted Critical
Publication of KR101865870B1 publication Critical patent/KR101865870B1/ko
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1276—RNA-directed DNA polymerase (2.7.7.49), i.e. reverse transcriptase or telomerase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07007—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07049—RNA-directed DNA polymerase (2.7.7.49), i.e. telomerase or reverse-transcriptase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/26—Endoribonucleases producing 5'-phosphomonoesters (3.1.26)
    • C12Y301/26004—Ribonuclease H (3.1.26.4)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/30—Phosphoric diester hydrolysing, i.e. nuclease
    • C12Q2521/327—RNAse, e.g. RNAseH

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
KR1020127001640A 2010-05-25 2011-05-25 변형된 ＲＮＡｓｅ Ｈ 및 핵산 증폭의 검출 Active KR101865870B1 (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US34798410P 2010-05-25 2010-05-25
US61/347,984 2010-05-25
PCT/KR2011/003811 WO2011149255A2 (en) 2010-05-25 2011-05-25 Modified rnase h and detection of nucleic acid amplification

Publications (2)

Publication Number Publication Date
KR20130021351A KR20130021351A (ko) 2013-03-05
KR101865870B1 true KR101865870B1 (ko) 2018-06-11

Family

ID=45004553

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1020127001640A Active KR101865870B1 (ko) 2010-05-25 2011-05-25 변형된 ＲＮＡｓｅ Ｈ 및 핵산 증폭의 검출

Country Status (6)

Country Link
US (2) US8618253B2 (https=)
EP (1) EP2576774B1 (https=)
JP (2) JP6008846B2 (https=)
KR (1) KR101865870B1 (https=)
CN (2) CN102918147B (https=)
WO (1) WO2011149255A2 (https=)

Families Citing this family (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20140255925A9 (en) * 2008-04-30 2014-09-11 Integrated Dna Technologies Modified rnase h enzymes and their uses
EP2644708B1 (en) 2008-04-30 2014-12-17 Integrated Dna Technologies, Inc. RNASE-H-based assays utilizing modified RNA monomers
US8911948B2 (en) * 2008-04-30 2014-12-16 Integrated Dna Technologies, Inc. RNase H-based assays utilizing modified RNA monomers
US9506057B2 (en) 2010-03-26 2016-11-29 Integrated Dna Technologies, Inc. Modifications for antisense compounds
AU2011299233B2 (en) 2010-09-07 2016-09-15 Integrated Dna Technologies, Inc. Modifications for antisense compounds
WO2012135053A2 (en) 2011-03-25 2012-10-04 Integrated Dna Technologies, Inc. Rnase h-based assays utilizing modified rna monomers
KR101753833B1 (ko) * 2012-01-09 2017-07-05 사회복지법인 삼성생명공익재단 절단 가능한 프로브를 이용한 만성골수성백혈병 유전자의 멀티플렉스 검출 방법
WO2013123440A1 (en) * 2012-02-17 2013-08-22 Institute For Systems Biology Rnase h-based rna profiling
CA2867435A1 (en) * 2012-03-19 2013-09-26 Integrated Dna Technologies, Inc. Modified rnase h enzymes and their uses
SG11201601890UA (en) * 2013-09-13 2016-04-28 Hoffmann La Roche Application of oligo-dt molecules to avoid generation of high molecular pcr products induced by polya-carrier
WO2015045593A1 (ja) 2013-09-30 2015-04-02 Ｎｅｃソリューションイノベータ株式会社 ターゲットの分析方法および分析キット
CN103966184A (zh) * 2014-05-06 2014-08-06 江西省科学院微生物研究所 一种实时热启动Taq酶及其制备方法
WO2016028671A1 (en) * 2014-08-19 2016-02-25 Bio-Rad Laboratories, Inc. Rna amplification methods
HK1243421A1 (zh) 2014-10-22 2018-07-13 Roka Bioscience, Inc. 检测核酸的组合物和方法
EP3287530A4 (en) * 2015-04-20 2018-11-21 KIM, Sung-Chun Method for analyzing biomolecule by using external biomolecule as standard material, and kit therefor
KR101901749B1 (ko) 2016-02-15 2018-09-28 주식회사 누리바이오 실시간 핵산 또는 단백질 검출용 단일핵산 및 이를 이용한 검출 방법
WO2017142297A1 (ko) * 2016-02-15 2017-08-24 주식회사 누리바이오 실시간 핵산 또는 단백질 검출용 단일핵산 및 이를 이용한 검출 방법
US11046940B2 (en) 2016-04-12 2021-06-29 Solis Biodyne Ou Synthetic reverse transcriptases and uses thereof
EP3448867A4 (en) * 2016-04-27 2019-11-27 Prominex, Inc. COMPOSITIONS AND METHOD FOR THE DETECTION OF NUCLEIC ACIDS
US11268117B2 (en) 2016-06-10 2022-03-08 Life Technologies Corporation Methods and compositions for nucleic acid amplification
CN110023507B (zh) * 2016-09-27 2024-01-23 纽丽生物科技有限公司 检测小rna或与小rna相关的蛋白质的方法
EP3673064A4 (en) 2017-08-24 2021-05-26 Takara Bio USA, Inc. METHOD OF PRODUCING NUCLEIC ACIDS USING STIMULUS MODIFIED OLIGONUCLEOTIDES
JP2019198259A (ja) * 2018-05-15 2019-11-21 株式会社島津製作所 １ステップｒｔ−ｐｃｒ法
KR102516307B1 (ko) * 2019-09-23 2023-04-03 주식회사 원드롭 RNase H 효소 및 이의 항체를 포함하는, 표적 핵산 증폭용 조성물 및 이의 용도
WO2022115822A2 (en) * 2020-11-03 2022-06-02 Northwestern University Methods for full-length reverse transcription pcr of long rnas containing modified ribonucleosides using a thermostable reverse transcriptase
JP2025503685A (ja) * 2022-01-12 2025-02-04 ドン，ショウリアン 核酸増幅の方法および組成物
CN120530204A (zh) * 2022-06-21 2025-08-22 苏州晶睿生物科技有限公司 核酸检测方法和系统
WO2025071292A1 (ko) * 2023-09-26 2025-04-03 주식회사 씨젠 핫 스타트 핵산 중합효소의 성능을 평가하는 방법

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2000004878A (ja) * 1998-05-29 2000-01-11 Qiagen Gmbh 熱安定性酵素の可逆性修飾の方法
JP2008004878A (ja) * 2006-06-26 2008-01-10 Dainippon Screen Mfg Co Ltd 基板処理方法および基板処理装置
WO2009135093A2 (en) * 2008-04-30 2009-11-05 Integrated Dna Technologies, Inc. Rnase-h-based assays utilizing modified rna monomers

Family Cites Families (26)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4965188A (en) 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4800159A (en) 1986-02-07 1989-01-24 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences
US4889818A (en) 1986-08-22 1989-12-26 Cetus Corporation Purified thermostable enzyme
US5079352A (en) 1986-08-22 1992-01-07 Cetus Corporation Purified thermostable enzyme
US5091310A (en) 1988-09-23 1992-02-25 Cetus Corporation Structure-independent dna amplification by the polymerase chain reaction
US5066584A (en) 1988-09-23 1991-11-19 Cetus Corporation Methods for generating single stranded dna by the polymerase chain reaction
US5075216A (en) 1988-09-23 1991-12-24 Cetus Corporation Methods for dna sequencing with thermus aquaticus dna polymerase
US5023171A (en) 1989-08-10 1991-06-11 Mayo Foundation For Medical Education And Research Method for gene splicing by overlap extension using the polymerase chain reaction
US5104792A (en) 1989-12-21 1992-04-14 The United States Of America As Represented By The Department Of Health And Human Services Method for amplifying unknown nucleic acid sequences
US5210015A (en) 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
US5338671A (en) 1992-10-07 1994-08-16 Eastman Kodak Company DNA amplification with thermostable DNA polymerase and polymerase inhibiting antibody
US5925517A (en) 1993-11-12 1999-07-20 The Public Health Research Institute Of The City Of New York, Inc. Detectably labeled dual conformation oligonucleotide probes, assays and kits
US5610066A (en) * 1993-12-10 1997-03-11 Amersham Life Science, Inc. Nucleic acid modifying proteins from Pyrococcus furiosus
US6787304B1 (en) 1994-12-28 2004-09-07 Georgetown University Fluorometric assay for detecting nucleic acid cleavage
EP0803058B1 (en) 1994-12-30 2008-11-05 Georgetown University Fluorometric assay for detecting nucleic acid cleavage
US20030165908A1 (en) 1994-12-30 2003-09-04 Georgetown University Fluorometric assay for detecting nucleic acid cleavage
US5773258A (en) * 1995-08-25 1998-06-30 Roche Molecular Systems, Inc. Nucleic acid amplification using a reversibly inactivated thermostable enzyme
US5853990A (en) * 1996-07-26 1998-12-29 Edward E. Winger Real time homogeneous nucleotide assay
US6951722B2 (en) * 1999-03-19 2005-10-04 Takara Bio Inc. Method for amplifying nucleic acid sequence
EA005739B1 (ru) * 2000-08-23 2005-06-30 Такара Био Инк. Способ амплификации нуклеиновых кислот
JP2006288400A (ja) * 2000-09-14 2006-10-26 Takara Bio Inc 耐熱性リボヌクレアーゼｈ
ATE411389T1 (de) 2000-09-14 2008-10-15 Takara Bio Inc Thermotolerante ribonuklease h
US8119336B2 (en) * 2004-03-03 2012-02-21 Ibis Biosciences, Inc. Compositions for use in identification of alphaviruses
WO2005123913A2 (en) * 2004-06-09 2005-12-29 Nustar Laboratory Reversibly modified thermostable enzyme compositions and methods of making and using the same

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2000004878A (ja) * 1998-05-29 2000-01-11 Qiagen Gmbh 熱安定性酵素の可逆性修飾の方法
JP2008004878A (ja) * 2006-06-26 2008-01-10 Dainippon Screen Mfg Co Ltd 基板処理方法および基板処理装置
WO2009135093A2 (en) * 2008-04-30 2009-11-05 Integrated Dna Technologies, Inc. Rnase-h-based assays utilizing modified rna monomers

Also Published As

Publication number Publication date
US9598729B2 (en) 2017-03-21
US8618253B2 (en) 2013-12-31
WO2011149255A2 (en) 2011-12-01
EP2576774A2 (en) 2013-04-10
JP6008846B2 (ja) 2016-10-19
WO2011149255A3 (en) 2012-05-18
CN102918147A (zh) 2013-02-06
EP2576774B1 (en) 2016-01-06
US20110294674A1 (en) 2011-12-01
CN102918147B (zh) 2017-04-12
CN106947749A (zh) 2017-07-14
WO2011149255A9 (en) 2012-08-16
US20140087968A1 (en) 2014-03-27
JP2013528384A (ja) 2013-07-11
JP2016146846A (ja) 2016-08-18
KR20130021351A (ko) 2013-03-05
EP2576774A4 (en) 2013-12-11

Similar Documents

Publication Publication Date Title
KR101865870B1 (ko) 변형된 ＲＮＡｓｅ Ｈ 및 핵산 증폭의 검출
JP2013528384A5 (https=)
EP2069487B1 (en) Zwitterionic detergents for the storage and use of dna polymerases
JP2011188856A (ja) リアルタイムｐｃｒのための核酸テンプレートの製造
KR20120046018A (ko) 단일 뉴클레오티드 다형성의 실시간 ｐｃｒ 검출
JP5022383B2 (ja) マグネシウム封鎖によるｐｃｒホットスタート
KR20120021263A (ko) Ｈｔｌｖ 바이러스 검출용 키트 및 이를 이용한 ｈｔｌｖ 바이러스의 검출 방법
US20120219945A1 (en) Use of single-stranded binding protein in amplifying target nucleic acid
CN104350160A (zh) 利用通过核酸外切酶和核酸内切酶二者切割的寡核苷酸探针进行的核酸检测
KR20120021262A (ko) 안정화된 프로브를 이용한 실시간 ｐｃｒ 검출
CN107109383A (zh) 不耐热性核酸外切酶
US20120045747A1 (en) Kit for detecting hepatitis b virus and method for detecting hepatitis b virus using the same
KR20120020067A (ko) 헤파티티스 ｃ 바이러스 검출용 키트 및 이를 이용한 헤파티티스 ｃ 바이러스의 검출 방법
KR102146523B1 (ko) 표적 핵산의 향상된 증폭
KR102516307B1 (ko) RNase H 효소 및 이의 항체를 포함하는, 표적 핵산 증폭용 조성물 및 이의 용도
KR20120021261A (ko) 살모넬라 종 및 대장균 ０１５７：ｈ７의 표적 서열을 동시에 증폭하는 방법 및 그를 위한 키트
US9637780B2 (en) Controlled inhibition and re-activation of DNA polymerases by cleavable oligonucleotide inhibitors
JP2011217746A (ja) 界面活性剤非含有ポリメラーゼ
KR20130092359A (ko) 인간 파필로마바이러스 검출용 올리고뉴클레오티드 세트
KR20120020066A (ko) Ｈｉｖ-1 검출용 키트 및 이를 이용한 ｈｉｖ-1의 검출 방법
US9157128B2 (en) Kit for detecting HIV-2 and method for detecting HIV-2 using the same
KR20120021267A (ko) 클라미디아 트라코마티스 종 검출용 키트 및 이를 이용한 클라미디아 트라코마티스 종의 검출 방법

Legal Events

Date Code Title Description
PA0105 International application

Patent event date: 20120119

Patent event code: PA01051R01D

Comment text: International Patent Application

PG1501 Laying open of application
A201 Request for examination
PA0201 Request for examination

Patent event code: PA02012R01D

Patent event date: 20160510

Comment text: Request for Examination of Application

E902 Notification of reason for refusal
PE0902 Notice of grounds for rejection

Comment text: Notification of reason for refusal

Patent event date: 20170510

Patent event code: PE09021S01D

E902 Notification of reason for refusal
PE0902 Notice of grounds for rejection

Comment text: Notification of reason for refusal

Patent event date: 20171127

Patent event code: PE09021S01D

E701 Decision to grant or registration of patent right
PE0701 Decision of registration

Patent event code: PE07011S01D

Comment text: Decision to Grant Registration

Patent event date: 20180423

GRNT Written decision to grant
PR0701 Registration of establishment

Comment text: Registration of Establishment

Patent event date: 20180601

Patent event code: PR07011E01D

PR1002 Payment of registration fee

Payment date: 20180604

End annual number: 3

Start annual number: 1

PG1601 Publication of registration
FPAY Annual fee payment

Payment date: 20210526

Year of fee payment: 4

PR1001 Payment of annual fee

Payment date: 20210526

Start annual number: 4

End annual number: 4

FPAY Annual fee payment

Payment date: 20220526

Year of fee payment: 5

PR1001 Payment of annual fee

Payment date: 20220526

Start annual number: 5

End annual number: 5

PR1001 Payment of annual fee

Payment date: 20230523

Start annual number: 6

End annual number: 6

PR1001 Payment of annual fee

Payment date: 20240523

Start annual number: 7

End annual number: 7