KR101769124B1 - Cosmetic Composition Containing Desalinized Magma Seawater, Ravendor extract, black bean extract, Hijikia fusiforme extract and Oryza Sativa Leaf/Stem Extract for Sensitive Skin - Google Patents
Cosmetic Composition Containing Desalinized Magma Seawater, Ravendor extract, black bean extract, Hijikia fusiforme extract and Oryza Sativa Leaf/Stem Extract for Sensitive Skin Download PDFInfo
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Abstract
The present invention relates to a composition containing a lavender extract, a lavender extract, a black bean extract, a tall extract and a rice leaf / stem extract as an active ingredient, so that the composition can provide a sensitive skin that can provide antioxidant, antiinflammatory, ≪ / RTI >
Description
The present invention relates to a sensitive skin cosmetic composition having excellent antioxidant, anti-inflammatory and redness-relieving effects, and more particularly to a composition for a sensitive skin cosmetic composition containing desalted lava sea water, lavender extract, black soybean extract, The present invention also relates to a cosmetic composition having an effect of relieving redness by protecting the skin from various stresses and antioxidation, anti-inflammation and atopic dermatitis.
Sensitive skin is a type of skin that reacts sensitively to stimuli. Its symptoms and cause are not clearly known, but it generally responds to external influences such as cosmetics, stress, and air pollution more sensitively than normal skin, It is skin that causes dermatitis well.
The stratum corneum of the skin is the first barrier between the skin and the external environment and is an important determinant of skin condition. The healthy stratum corneum contains 15 ~ 20% of water. When the moisture falls below 10%, the skin becomes dry, the gloss and elasticity are decreased, and the wrinkles are increased. The extrinsic factors such as penetration of irritants and the endogenous factors such as aging, hormones, When defects are caused by factors, the resistance is lowered, resulting in deterioration of the function of the epidermal lipid bilayer and increased sensitivity to stimulants, resulting in sensitive skin and dry skin.
The main symptoms of sensitive skin are redness, obtuse skin, scars, or skin diseases such as contact dermatitis, eczema, and acne. Among these, redness is a phenomenon that the skin gets reddened. It is the most common skin reaction that is caused by various internal or external stimuli.
Reversible reactions in sensitive skin may be followed by inflammatory pathways such as contact irritant salts, allergies, atopy and the like, along the path of allergic reactions. Atopic dermatitis occurs mainly as the content of ceramides in the stratum corneum is decreased. As the water retention function of the stratum corneum is lowered, the skin is dried and a large amount of keratin is produced. As the skin barrier function is lowered, the stimulant- And induce the stimulation, so that the antigen protein which is difficult to intrude into the normal skin invades, and the skin allergy and the like are issued, resulting in severe pruritus.
As mentioned above, it is recommended to use products containing ingredients such as anti-inflammatory, antihistamine, anti-edema, licorice, allantoin, chamomile, etc. to relieve sensitive skin symptoms caused by various causes, There are very few basic data on the treatment and the factors related to the treatment.
As an example of such cosmetics, Korean Patent Laid-Open Publication No. 10-2011-0030812 (published on Mar. 24, 2011) discloses a cosmetic composition comprising an extract of Chrysanthemum indicum, an ammonium glycyrrhizate / casein / zinc gluconate / marroni extract, Or a mixture thereof, which has an effect of preventing and improving inflammation and relieving itching, is disclosed.
Korean Patent Laid-Open Publication No. 10-2005-0001872 (published on Jan. 01, 2012) discloses a cosmetic composition for sensitive skin having skin moisturizing effect and skin irritation alleviating effect containing hot spring water and Puerariae Radix extract.
Also, Korean Patent Registration No. 10-1081178 (registered on November 11, 2011) discloses a sensitive cosmetic composition having a skin irritation and inflammation relieving effect containing a citrus extract of Citrullus vulgaris.
However, in the case of the conventional sensitive cosmetic composition based on the anti-inflammation and skin irritation mitigation described above, there is no patent for a sensitive cosmetic composition which provides antioxidant, anti-inflammatory, red ginseng relief and atopic dermatitis improving effect together.
The present invention relates to a sensory cosmetic composition which is capable of providing antioxidative, antiinflammatory, red ginseng mitigation and atopic dermatitis improving effects by containing desalted lava sea water, lavender extract, black soybean extract, top extract and rice leaf / stem extract as active ingredients To provide a composition.
The present invention provides a sensitive cosmetic composition having excellent antioxidative, anti-inflammatory, redness-reducing and atopic dermatitis-improving effects containing desalted lava sea water, lavender extract, black bean extract, top extract and rice leaf / stem extract as active ingredients.
According to a preferred feature of the present invention, the desalted lava waters are desalinated by removing univalent ions using an ion exchange membrane by dialysis.
According to a preferred aspect of the present invention, the lavender extract, black bean extract, chrysanthemum extract, and rice leaf / stem extract are each individually extracted from individual crushed lavender, black bean, Extract.
According to a preferred aspect of the present invention, the lavender extract, black bean extract, chrysanthemum extract, and rice leaf / stem extract are selected from the group consisting of water, an anhydrous or lower alcohol having 1 to 4 carbon atoms, acetone, acetyl acetate, butyl acetate and 1,3- Glycol and the like.
According to a preferred aspect of the present invention, the desalted lava sea water, lavender extract, black bean extract, chrysanthemum extract, and rice leaf / stem extract comprise 10 to 30 wt% of lava seawater, 50 to 80 wt% of lavender extract, 0.01 to 20 wt% 0.01 to 20% by weight of top extract and 0.01 to 20% by weight of rice leaves / stems.
According to a preferred aspect of the present invention, the mixed complex of the desalted lava sea water, lavender extract, black bean extract, top extract and rye / stem extract shows antioxidative, anti-inflammatory, red ginseng mitigation and atopic dermatitis improving effect.
According to a preferred aspect of the present invention, the sensitive cosmetic composition containing the desalted lava sea water, the lavender extract, the black bean extract, the chrysanthemum root extract, the chrysanthemum extract, and the leaf / stem extract may be used as a softening agent, a nutritional lotion, a nutritional cream, a massage cream, A cream, a cleansing cream, a cleansing foam, a cleansing water, or a pack.
The cosmetic composition containing desalted lava water, lavender extract, black bean extract, top extract, and rice leaf / stem extract for sensitive skin according to the present invention has sensitivity to antioxidant, antiinflammation, redness and alleviation of atopic dermatitis, A cosmetic composition for skin can be provided.
According to the cosmetic composition sensitive to the present invention, it was confirmed that there was no intracellular toxicity in the cytotoxicity test through the MTT assay, and skin irritation test showed no skin irritation, so that it was produced as a harmless and highly safe sensitive cosmetic product can do.
1 is a graph showing the degree of cytotoxicity according to the concentration of a sensitive cosmetic composition containing desalted lava water, lavender extract, black bean extract, top extract, and rice leaf / stem extract according to a preferred embodiment of the present invention.
FIG. 2 is a graph showing the antioxidative effect according to concentration of a desensitizing cosmetic composition containing desalted lava sea water, lavender extract, black soybean extract, top extract, and rice leaf / stem extract according to a preferred embodiment of the present invention.
FIG. 3 is a graph showing the anti-inflammatory effect according to the concentration of a desensitizing cosmetic composition containing desalted lava water, lavender extract, black bean extract, top extract, and rice leaf / stem extract according to a preferred embodiment of the present invention.
FIG. 4 is a graph showing the effect of using a cosmetic composition showing a soothing effect on the redness of redness of a cosmetic composition containing desalted lava water, lavender extract, black bean extract, top extract and rice leaf / stem extract according to a preferred embodiment of the present invention It is a facial photograph.
Hereinafter, the preferred embodiments of the present invention and the physical properties of the respective components will be described in detail with reference to the accompanying drawings, in order that the present invention can be easily practiced by those skilled in the art , And this does not mean that the technical idea and scope of the present invention are limited.
The present invention relates to a sensitive cosmetic composition containing, as an active ingredient, desalted lava sea water, lavender extract, black bean extract, top extract and rice leaf / stem extract.
Here, lava seawater is an underground seawater that flows through the basalt layer of Jeju Island and flows into the underground of the mainland. It is mainly buried in the northeast of Jeju. It contains high concentration of minerals. It is purified and purified through the process of penetrating through the basalt. . Lava seawater is characterized by stability with low daily / seasonal fluctuation and physical change, cleanliness without detection of pathogen phenolics, and functional and non-toxic safety characteristics through functional minerals such as magnesium and calcium. , Cosmetics, foods and the like.
Lavender is a 25 to 30 species of plants belonging to the genus Lepidoptera, Lepidoptera, and Labanudra, planted for flowering in flowers and plants, and planted for ornamental purposes. Perfume is used as a source of fragrance and cosmetics and is also used to treat headaches and nervousness. Lavandula officnalis or Lavandula angustifolia Paper is mainly used in aromatherapy. Lavender essential oil is a raw material of pomade. When diluted, it rubs on scalp and promotes hair growth. Lavender water extracted from distillation is effective for skin cleansing and wound treatment. It is also effective in sunburn and damaged skin.
Black bean refers to black bean with dark green color. It has a similar nutrient content compared to ordinary soybean, but it has 4 times more antioxidant ingredient, and it is known to prevent adult diseases and to have a diet effect. Cysteine, which is essential for hair growth, is contained to prevent hair loss. Anthocyanins belonging to the flavonoid family exhibit red, purple green, and other colors depending on the hydrogen ion concentration of the plant. It is known to have an ultraviolet ray blocking action and an insect intrusion defense action from ultraviolet rays, and also acts as an antioxidant to remove active oxygen.
It is a perennial seaweed, which is a seaweed belonging to the mackerel and subspecies and sprouting from the root to the fall and repeating yearly. Jeju and South coast are the production centers. Tott is not only a good source of dietary fiber, but also contains a large amount of laminaran and fucoidin, which are known to have functions such as blood coagulation and immunity enhancement, and other antioxidative and antimicrobial activities. And it is known that it has high efficacy in prevention and treatment of various adult diseases and chronic diseases due to its high content of minerals. In addition, it has been confirmed that there is anti-cancer effect as well as prevention of deposition of cholesterol in the blood vessels and smooth movement of the intestinal tract, thereby being effective for discharging heavy metals.
Rice is a perennial herb and belongs to a visa plant which is enclosed by the earthen of the plant taxonomic seed plants. Rice grows and blooms in the main stem and primary tiller branches (the branches come out from the nodes in the soil of the plant). The stem is made between the nodes and nodes. The leaves are divided into leaf sheath and leaf sheath, with leaf veins and leaf ears in between. It is a food source widely cultivated all over the world, its fruit is edible, and the ryegrass is used as livestock food.
In the present invention, " lava sea water " is defined in Korean Patent No. 08532244 (Application No.: 10-2008-0027861), entitled " Method for producing mineral composition and mineral composition obtained by the method & It is synonymous with the "saltwater underground in the eastern part of Jeju Island" as described. Specifically, it is based on the classification of groundwater in Cheju Island based on the salinity concentration used in the papers (Meat Garden, etc., Study on Hydrological Lipid in Eastern Region of Cheju Island, 2003, Kyungwon University) It can be understood as meaning low-salt groundwater (electric conductivity of 1,700 / cm to 17,350 / cm) and brine groundwater (electric conductivity of 17,350 / cm or more), but preferably, electric conductivity of 17,350 / It can be understood that it means a brine groundwater having a salt concentration of 30 (permilliage) or more (usually, the saline concentration of seawater is 32 to 35).
The above-described desalted lava waters can be produced by a desalting step in which monovalent ions are removed using an ion exchange membrane by dialysis.
In the present invention, the extract is selected from the group consisting of individual extracts individually extracted from individually crushed lavender, black bean extract, top extract, And each extract may be composed of a composite composition adjusted to various mixing ratios.
The extract may also be extracted with a solvent in the range of from 8 to 12 times the dry weight of the solvent at a temperature of from 40 to 100 ° C for 1 to 24 hours or from 5 to 40 ° C for 1 to 20 days of extraction, And can be extracted for 5 to 10 hours. In particular, the extract is preferably extracted at a temperature of 50 DEG C for 12 hours using a solvent having a dry weight of 8 to 12 times the volume.
The above-mentioned extract is preferably extracted with any one solvent selected from the group consisting of water, an anhydrous or a lower alcohol having 1 to 4 carbon atoms, acetone, acetyl acetate, butyl acetate and 1,3-butylene glycol
Preferably, the solvent used for extracting the extract is water, an organic solvent, or a mixture thereof, but is preferably an aqueous ethanol solution or water, wherein the aqueous ethanol solution has a mass concentration of 40 to 70%, particularly 50% Is preferable
The complex composition used in the cosmetic composition according to the present invention is characterized in that 10 to 30% by weight of desalted lava sea water, 50 to 80% by weight of lavender extract, 0.01 to 40% by weight of black soybean extract, 20 to 20% by weight of the root extract, 0.01 to 20% by weight of the root extract and 0.01 to 20% by weight of the leaf / stem.
Hereinafter, embodiments of the present invention will be described.
However, the following examples are illustrative of the present invention, and the contents of the present invention are not limited by the following examples.
Example 1: Preparation of the composite composition according to the present invention]
Example 1-1: Preparation of desalted lava water
This example was intended to prepare lava water for use in cosmetic compositions through desalination. Lava seawater was collected in the eastern part of Jeju Island and the impurities were filtered with a 0.2 μm filter and desalted by an electrodialyzer (ASTOM CO. LTD., Japan) using an ion exchange membrane to remove monovalent ions.
Example 1-2: Preparation of lavender extract, black soybean extract, goat extract and rice leaf / stem extract
Lavender, black soybean, green tea, and rice leaves / stems, respectively, were thoroughly dried and then pulverized to obtain 100 g of each of lavender, black bean, green tea, and rice bran / stem. 1,000 g of each of lavender, black bean, green tea, and rice bran / pulverized product was immersed in a 10-fold volume of ethanol solution (mass concentration of 50 g) for 12 hours at a temperature of 50.degree. Each of the extracts was filtered with filter paper, and the residue was further extracted three times by the same method. Each extract was concentrated under reduced pressure at 50 ?.
Example 1-3: Preparation of the composition according to the present invention
10 to 30 g of the desalted lava sea water prepared above, 50 to 80 g of concentrated lavender extract, 0.01 to 20 g of black soybean extract, 0.01 to 20 g of top extract and 0.01 to 20 g of a rice leaf / stem extract can be mixed. 20g of lava sea water, 60g of lavender extract, 7g of black soybean extract, 7g of candle extract, and 6g of rye / stem extract were mixed.
[Experimental Example 1: Cytotoxicity of the composition of the present invention]
Human fibroblast (CCD-986SK) was cultured in an ATCC (American Type Culture Collection) in order to examine the skin cell survival rate of the composition prepared in Example 1-3. Each cell was inoculated into a 24-well culture plate at a concentration of 1 × 10 4 cells / ml. The medium was DMEM (Dubelco's Modified Eagle Medium, BRL, USA) containing 10% FBS. After culturing for 48 hours in DMEM containing 10% FBS and culturing for 25-30% of the surface area of the culture dish, the cells were further replaced with FBS-free DMEM containing the composition prepared in Example 1-3 and incubated for 24 hours. 50 μl of a solution of 2.5 mg / ml of 3- (4,5-dimethylthiazol-2-yl) -2,5-diphenyltetrazolium bromide (MTT, Sigma M5655, USA) (DMSO, Sigma D2650, USA) solution was added to each well and the mixture was stirred for 20 minutes to dissolve the formed formazan crystals. 100 μl of the solution was added to 96 wells And the absorbance at 570 nm was measured by Enzyme-Linked Immunosorbent Assay (ELISA). The survival rate of skin cells was calculated by the following equation based on the absorbance of the control using pure water and expressed as a percentage. The results are shown in Table 1 below.
The test results of the effect on the cytotoxicity using the human fibroblasts are shown in Table 1 and also shown in the graph in Fig. As shown in FIG. 1, when the composition of the present invention was treated by concentration, it was confirmed that there was no cytotoxicity.
[Experimental Example 2: Antioxidant effect test of the composition of the present invention]
DPPH (2,2-Diphenyl-1-picrylhydrazyl) measurement method was performed to confirm the antioxidant effect of the composition prepared in Example 1-3. The DPPH assay is a method of measuring the absorbance at 540 nm to the extent that the inhibitor decolorizes stable radical DPPH. The used samples are shown in Table 2 below, and the free radical scavenging activity was measured by using the following Equation 2, and is shown in Table 3 and in the graph of FIG.
A: Abs of experimental group
B: Abs blank of experimental group
C: Abs of control group
D: Abs of blank of control group
The control group was Epigallocatechin gallate (EGCG). Epicalocatechin karate is a kind of polyphenol which is an extract of green tea leaves and is known to have a strong antioxidant activity.
[Experimental Example 3: Anti-inflammatory effect test of the composition of the present invention]
In order to confirm the anti-inflammatory effect of the compositions prepared according to Examples 1-3, the anti-inflammatory effect was tested by measuring the activity of interleukin-6.
Interleukin-6 is an enzyme that acts as a diverse chemical mediator involved in inflammatory and allergic reactions in vivo, and substances that inhibit interleukin-6 inhibit the production of various chemical mediators. That is, the higher the inhibitory effect of interleukin-6, the better the anti-inflammatory effect. In the present test, the degree of activity of interleukin-6 was measured by using substrate and enzyme.
Experiments were conducted using the samples shown in Table 4 below, and the degree of interleukin-6 activity was measured using the equation (3). The control group was ultraviolet B (UVB), which causes inflammation or blistering of the skin.
The experimental results are shown in Table 5 below and also shown in FIG. 3 as a graph.
As shown in FIG. 3, the composite composition of desalted lava sea water, Lavender extract, black soybean extract, top extract, and rice leaf / stem extract showed lower levels of interleukin-6 activity, that is, higher anti-inflammatory effect at all concentrations.
[Formulation Example 1: Preparation of cosmetic composition according to the present invention]
The lotion formulation is a cosmetic product containing the desalted lava sea water, lavender extract, black bean extract, top extract, and rice leaf / stem extract of the present invention as an active ingredient by the method of Formulation Example 1 and
[Experimental Example 4: Test for soothing effect due to reduction of redness]
In order to analyze the efficacy of the compositions prepared in Examples 1-3, 42 women who were prepared according to Formulation Example 1 and fertilizer Formulation Examples 1 to 5, The soothing effect of redness relaxation was evaluated.
The subjects were prohibited to use functional cosmetics and basic cosmetics such as eye cream, anti-aging cream, etc., which could affect the test results, and did not use packs or massages. Experiments were carried out for 4 weeks at room temperature and humidity (45 ± 5%) at room temperature and humidity for 30 min after morning cleansing. Spectrophotomoter CR-2600D, Konica Minolta, Japan) and a panoramic imaging system (Robo Skin Analyzer CS50, Inforward, Inc., Japan).
After stabilizing under the above conditions, an appropriate amount of lotion formulations of Formulation Example 1 and Comparative Formulation Examples 1 to 5 was taken and spread evenly over the face portion and absorbed. Measurements with a spectrophotometer and a panoramic system were made prior to the use of the test substance and after two weeks and after four weeks.
In the evaluation by the spectrophotometer, the left ball region was measured three times in succession, and the average value was calculated. The L * value is the brightness, the a * value is the redness, and the b * value is the yellowness. The measured value of the spectrophotometer is composed of three factors L *, a * and b * . The a * value indicating the redness of the skin was used for the analysis to determine the sedative effect of the redness of the test substance, and as the measured value decreased, . Evaluation of the improvement effect of the soothing effect by the red flag relaxation is shown in Table 7 after measurement using the following equation (3).
The evaluation by the panoramic system measured all the subjects' faces in the same position as the uniform illumination for consistent shooting by the same test person. In order to secure the hair line of the subject, the hair was fixed with a hair band, then the same top gown was put on the chair and the face was fixed on the top of the tool bar under the cage, Respectively.
As a result of analyzing the improvement effect of sedation by redness relaxation of the left ball area using a spectrophotometer and a panoramic photographing system, it was found that the a * value indicating the redness of the skin was higher in the group to which Formulation Example 1 was applied It was improved to 7.10% after 2 weeks of use and to 16.33% after 4 weeks of use, and it was confirmed that the improvement rate against redness relaxation was superior to
[Experimental Example 5: Experiment for improving atopic dermatitis]
Sixty male and female subjects having symptoms of atopic dermatitis over 5 years of age were divided into 6 groups. Each of the groups was divided into two groups: Formulation Example 1 and Comparative Formulation Examples 1 to 5 containing the composition prepared in Example 1-3, The SCORAD Index (SCORAD Index, developed by the European Task Force on Atopic Dermatitis, which shows a higher value with increasing severity of atopic symptoms) is shown in Table 8, Using the equation (4), the results are shown in Table 9.
In Table 8, it was confirmed that the cosmetic composition of Example 1-3 had better atopic dermatitis improving activity than the use of the cosmetic composition of Comparative Formulation Example.
[Experimental Example 6: Skin Adverse Reaction Evaluation Test]
In order to analyze the efficacy of the composition prepared in Example 1-3, the skin irritancy of the composition was evaluated in 42 women who met Formulation Example 1 and Comparative Formulation Examples 1 to 5 according to the selection criteria of the subjects. 15 [mu] l of cosmetic composition of Formulation Example 1 and Comparative Formulation Examples 1 to 5 lotion formulation was applied to a Finn chamber for the test of the test subjects and applied for 24 hours. After the pin chamber was removed, 30 minutes later, (ICDRG, International Association of Contact Dermatitis Research) and the American Society of Cosmetic Dermatology (ICDRG), and the American Society for Cosmetic Dermatology PCPC, Personal Care Products Council) safety evaluation guidelines. The evaluation value was calculated as a stimulation index using Equation (4), and the results are shown in Table 8 below.
* Judgment score
-: No reaction (irritation degree: 0)
±: Suspicious or mild reaction (degree of irritation: 0.5)
+: Slight erythema (degree of irritation: 1)
++: marked erythema that may include partial papules and erythema (degree of irritation: 2)
+++: marked erythema with diffuse edema (degree of irritation: 3)
++++: Severe erythema including diffuse edema with blisters (degree of irritation: 4)
* Stimulation index
0 = judgment score <0.02: no stimulation
0.02 = judgment score <0.25: low stimulus
0.25 = judgment score <1: light stimulus
1 = judgment score <2.5: moderate stimulus
2.5 = judgment score: strong stimulus
From the results of the skin irritation test,
The cosmetic composition containing the complex composition of the above-mentioned lava sea water extract, lavender extract, black bean extract, top extract and rye / stem extract can be used in the production of usual cosmetics without skin irritation, In an amount of 0.001 to 30% by weight.
The cosmetic composition containing the composite composition described above may be prepared in any form conventionally produced in the art and may be in the form of a solution, a suspension, an emulsion, a paste, a gel, a cream, a lotion, a powder, a soap, Containing cleansing, oil, and the like, but is not limited thereto.
The cosmetic formulations containing the above-described composite composition may be formulated as a softening agent, a nutritional lotion, a nutritional cream, a massage cream, an ampoule, an eye cream, a cleansing cream, a cleansing foam, a cleansing water or a pack.
Ample cosmetic compositions and cream cosmetic compositions were prepared as follows using the composite composition obtained by the above-mentioned process for producing a composite composition.
[Formulation Example 2]
Formulation Example 2-1: Preparation of ampule cosmetics
The ingredients shown in Table 6 below, which contained the oil component and the water component, were mixed by heating at 75? And emulsified. The mixture was cooled to room temperature to prepare an ampule cosmetic composition containing the composite composition of the present invention.
Formulation Example 2-2: Preparation of cream cosmetic
The components shown in Table 8 below containing the oil component and the water component were mixed by heating at 75 ° C. and emulsified and then cooled to room temperature to prepare a lotion cosmetic composition containing the composite composition of the present invention with desalted lava water and extract.
The foregoing description is merely illustrative of the technical idea of the present invention, and various changes and modifications may be made by those skilled in the art without departing from the essential characteristics of the present invention. Therefore, the embodiments disclosed in the present invention are intended to illustrate rather than limit the scope of the present invention, and the scope of the technical idea of the present invention is not limited by these embodiments. The scope of protection of the present invention should be construed according to the following claims, and all technical ideas within the scope of equivalents should be construed as falling within the scope of the present invention.
Claims (7)
Wherein the extract is selected from the group consisting of water, an anhydrous or a lower alcohol having 1 to 4 carbon atoms, acetone, ethyl acetate, butyl acetate and 1,3-butylene glycol, Cosmetic composition.
A cosmetic composition for improving sensitive skin having a formulation of one of softening lotion, nutritional lotion, nutritional cream, massage cream, essence, ampule, eye cream, mask sheet, cleansing cream, cleansing foam, cleansing water and pack.
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