KR101754411B1 - Manufacturing method of vegetable worms liquor - Google Patents

Abstract

The present invention relates to a method for preparing a seedling-producing microorganism, which comprises cultivating a microorganism on a brown rice medium, incubating the microorganism, culturing the microorganism, and cultivating the microorganism by inoculating it into a primary culture medium. A medium injection step of transferring a glass bottle into an automatic injector to make a secondary medium by injecting brown rice, 0.05-1 g of ginseng and 7-11 g of purified water per 10 g of brown rice, A sealing step of sealing the opening of the glass bottle with a cover; A sterilization step in which a glass bottle is placed in a sterilizer and sterilized at 100 to 150 ° C; A cooling step in which the glass bottle is transferred to a cooling chamber and cooled at a temperature of 10 to 20 DEG C for 10 to 20 hours; An inoculation step of inoculating the insecticide liquid seedlings into a secondary culture medium inside the glass bottle after opening the cover on a clean bench of a clean room; Culturing the insects of the vials by covering them with a cover and transferring them to a culture room made up of dark rooms and incubating the caterpillar fungus for 5 to 10 days at a temperature of 22 to 26 캜; Growing the caterpillar fungus for 30 to 50 days while maintaining the temperature in the cultivation room at a temperature of 15 to 21 캜 and a humidity of 60 to 70%; And removing the lid, injecting alcohol into the glass bottle, and sealing the lid.

Description

TECHNICAL FIELD [0001] The present invention relates to a method for manufacturing a caterpillar fungus,

More particularly, the present invention relates to a method for preparing a Chinese cabbage (Chinese cabbage) sausage by cultivating the Chinese cabbage seedlings in a medium containing brown rice and ginseng and cultivating the same.

Generally, it is a medicinal mushroom that enters the insect body of the host and forms fruiting body through the nutrients of the host. The taxonomic position corresponds to the acanthopanax spp., And the Asian area such as Korea, Japan, And is widely distributed throughout the world.

In winter, it is insect. In summer, it is named as Cordyceps in order to break through the host and turn into mushroom. It is named according to host such as silkworm, pupa, bee, dragonfly, beetle, , The fruit bodies are well formed in various host plants.

The nutrient source of Chinese caterpillar fungus is the nutrient conversion of living insects. It uses animal protein as a nutritional ingredient, so it contains useful materials not found in ordinary mushrooms through various physiological active substances.

Such caterpillar fungus has recently been successfully cultivated in Korea, and has been recognized as one of the three major herbal medicines along with ginseng and antler, and has been developed as an anti-cancer drug called cordisepin, which is isolated from Chinese caterpillar fungus. In addition, it has been well known through various studies that cordyceps contains a large amount of industrially available substances such as polysaccharides, steroids and enzymes, and has pharmacological effects such as immunity enhancement, anti-cancer, inflammation inhibition, anti- have.

In this way, it is difficult to use it because it is difficult to artificially cultivate it because of its natural habitat. Recently, artificial cultivation and mass cultivation have succeeded.

For example, Patent Document 1 discloses a method of artificial cultivation using silkworm or silkworm pupa as a host. In other words, a method of producing funguscoptera by forming a fruiting body in a pupa formed by the mycelial formation of the mycelium by inoculating the spore of Pesylomyces japonica, a kind of Cordyceps, as a sprayer on the surface of the larvae or pupa of the 4th to 5th instar larvae of silkworm to be.

Patent Document 2 discloses a method for producing Cordyceps sinensis by percutaneous inoculation of a spore of Pseudomycetes sp. Strain J300 into a 5th instar larva of a silkworm larvae using a sprayer and then forming a fruiting body in a pupa formed with a mycelium have.

However, since the above-mentioned conventional techniques use living silkworm as a host, they can be used only twice in spring and summer, and the germination rate of the seedlings is extremely low. After the inoculation of the silkworm, the cocoon is infected and the cured pupa is selected There is a problem that cultivation is troublesome because several steps for forming fruiting bodies under appropriate temperature and humidity are required.

In addition, since the yield of the pupa having infected mycelia is low due to infected silkworms, and all the pupa prepared for fruiting body formation does not produce cordyceps, it is difficult to obtain good quality products as the various stages progress, There is a problem of deterioration.

Therefore, the above-mentioned conventional techniques have a limitation in mass production of caterpillar fungus, increase in cultivation cost, and low production efficiency.

Korean Patent Registration No. 10-0187897 (published on June 1, 1999) Korean Patent Registration No. 10-0187895 (published on June 1, 1999)

The present invention is conceived to solve the above-mentioned problems, and it is an object of the present invention to solve the above-mentioned problems, and it is an object of the present invention to provide a method for producing a Chinese cabbage, The present invention aims to provide a method of manufacturing a Chinese cabbage soap having high productivity and efficiency by allowing optimal regulation of moisture and nutrients in the medium to have optimal conditions for the soaking.

In addition, it is possible to control light brightness and maintain temperature and humidity properly, so that it has the optimum conditions necessary for growth of Chinese caterpillar fungus, so that it has excellent foot and growth of Chinese caterpillar fungus, has uniform occurrence of Chinese caterpillar fungus, The object of the present invention is to produce a sake using a Chinese caterpillar fungus.

In order to solve the above problem, the present invention relates to a method for preparing a first medium as a liquid seed medium, sterilizing the seed medium at 100 to 150 ° C for 20 to 30 minutes, cooling the seed medium, inoculating the first seed medium to the first medium, A seed preparation step for making liquid seeds; A medium injection step of transferring a plurality of glass bottles stored in a storage box to an automatic injector to automatically quantitatively inject brown rice, 0.05-1 g of ginseng and 7-11 g of purified water per 10 g of brown rice to form a secondary culture medium; A sealing step of covering the entrance of the glass bottle with a cover made of a bundle of cotton sausage and sealing it; A sterilization step in which a glass bottle is placed in a sterilizer and sterilized at 100 to 150 ° C; A cooling step in which the glass bottle is transferred to a cooling chamber and cooled at a temperature of 10 to 20 DEG C for 10 to 20 hours; An inoculation step of inoculating the insecticide liquid seedlings into a secondary culture medium inside the glass bottle after opening the cover on a clean bench of a clean room; Incubating the inlet of the glass bottle with a cover, transferring the glass bottle to a culture room made of a dark room, and culturing the mycelium of the mildew organisms for 5 to 10 days at a temperature of 22 to 26 캜; When the surface of the hyphae that initially become beige in the culturing step becomes gradually darker and becomes orange or orange, the cultivation step is carried out. In the cultivation room, a temperature of 15 to 21 캜, a humidity of 60 to 70% A cultivation step of growing fruit bodies by growing the Cordyceps sinensis for 30 days to 50 days while maintaining the brightness of light of 2,000 lux; And a lyophilization step of injecting alcohol into the glass bottle in which the body is formed and sealing the lyophilized body, wherein the primary culture medium comprises 10 to 20 g of sugar, 10 to 20 g of malt extract, 10 to 20 g of dextrose, Yeast extract, 3 to 7 g of yeast extract, 1 to 5 g of arginine, 1 to 5 g of serine, 0.5 to 2 g of nitroglyme, 0.01 to 0.1 g of biotin, 0.01 to 0.1 g of niacin, 0.01 to 0.1 g of ascorbic acid, 0.01 to 0.1 g of NaCl, 0.01 to 0.1 g of FeCl _3, 0.01 to 0.1 g of K ₂ HPO _4, 0.01 to 0.1 g of MgSO _{4 and} 0.01 to 0.1 g of CaCl ₂ , When the temperature of the cultivation room is raised to a temperature of 22 to 26 DEG C, the head portion of the fruiting body is made white for 5 to 10 days, and then the temperature of the cultivation room is again raised to a temperature of 15 to 21 DEG C To form a plurality of branches on the body, wherein the branching step is repeated a plurality of times Performed provides a process for the preparation of a drink it characterized in that the fungus so that a plurality of the enriched form in a fruit body.

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According to the present invention, it is possible to cultivate and harvest the Chinese cabbage, and to appreciate the unique flavor and flavor of brown rice and ginseng, It is highly effective in productivity and efficiency by adjusting the moisture and nutrients appropriately to have the optimal conditions for the activation.

In addition, it is possible to control light brightness and maintain temperature and humidity properly, so that it has the optimum conditions necessary for growth of Chinese caterpillar fungus, so that it has excellent foot and growth of Chinese caterpillar fungus, has uniform occurrence of Chinese caterpillar fungus, There is an effect that a sake can be produced by using Cordyceps.

1 shows a flowchart of a manufacturing method according to an embodiment of the present invention.
2 is a view showing a state in which a glass bottle for insect harvesting according to an embodiment of the present invention is housed in a storage box.
FIG. 3A is a culture photograph of a primary culture medium according to an embodiment of the present invention. FIG.
FIG. 3B is a photograph showing a glass bottle filled with a secondary culture medium according to an embodiment of the present invention.
FIG. 3c is a photograph showing a secondary culture medium inoculated with Cordyceps specimens according to an embodiment of the present invention.
4 (a) and 4 (b) are views showing a glass bottle covered with a cover according to an embodiment of the present invention.
5A is a photograph showing a foot of Cordyceps sinensis according to an embodiment of the present invention.
FIG. 5B is a photograph showing a state roll in which a plurality of branches are formed on a fruiting body according to an embodiment of the present invention.
6 is a cross-sectional view illustrating a state in which caterpillar fungus is cultivated according to an embodiment of the present invention,
7 is a photograph showing a growth of fruit bodies of Cordyceps sinensis according to an embodiment of the present invention.

Hereinafter, with reference to the drawings, specific details for carrying out the method for manufacturing a Chinese cabbage syrup according to the present invention will be described in detail with reference to examples.

The method for preparing the Chinese cabbage (Chinese cabbage) according to the present invention is prepared by inoculating Chinese cabbage seeds into a brown rice medium and cultivating the cabbage. The preparation step S10, the medium injection step S20, the sealing step S30, (S40), a cooling step (S50), an inoculation step (S60), a cultivation step (S70), a cultivation step (S80), and a syringe injection step (S90).

The seedling preparation step (S10) is a step of preparing a Chinese cabbage seedlings by inoculating them into a primary culture medium and culturing the seedlings.

It starts from the stage of producing the Cordyceps mushroom Lactobacillus by flattening and expanding the spore-separated cornflower seeds after receiving or collecting the seeds from the seedpings. There are solid seeds cultured using solid medium and liquid seeds cultured by dissolving predetermined nutrients in water. However, since solid seeds are inoculated on the surface of the culture medium and grow on the surface of mycelium, In the present invention, liquid seeds are used.

As shown in FIG. 3A, a primary culture medium, which is a liquid seed culture medium, was prepared, sterilized at 100 to 150 ° C for 20 to 30 minutes, cooled to 20 ° C, and seeded with Chinese cabbage seeds. And cultivated to produce a Chinese cabbage liquid seed.

At this time, the primary culture medium was prepared by adding 10 to 20 g of sugar, 10 to 20 g of malt extract, 10 to 20 g of dextrose, 3 to 7 g of yeast, 3 to 7 g of peptone, 3 to 7 g of yeast extract, 1 to 5 g of arginine, 0.5 to 2 g of nitrite, 0.01 to 0.1 g of biotin, 0.01 to 0.1 g of niacin, 0.01 to 0.1 g of ascorbic acid, 0.01 to 0.1 g of NaCl, 0.01 to 0.1 g of FeCl _3, 0.01 to 0.1 g of K ₂ HPO ₄ g, 0.01 to 0.1 g of MgSO _{4, and} 0.01 to 0.1 g of CaCl ₂ , so that carbon sources, nitrogen sources, vitamins, inorganic salts and the like are uniformly contained as nutrients. As a result, the primary culture medium has a high productivity and efficiency by appropriately controlling the moisture and nutrients to have the optimal conditions for the growth.

In the step of injecting the medium (S20), a glass bottle is moved to an automatic injector, and a second medium is prepared by injecting brown rice, ginseng and purified water. The medium is suitably adjusted to have moisture and nutrients Productivity and efficiency are high.

In particular, the use of a secondary medium containing ginseng in which a large number of saponins are contained in addition to brown rice, saponin adsorbs ingredients harmful to cultivation, reduces toxicity, and induces vigorous growth of cordyceps, The whole surface is grown in a condition that the fruiting body is completely covered, the mycelial growth is quick, and the formed fruiting body can form a healthy fruiting body similar to that found in the natural state.

As shown in FIG. 3B, a glass bottle having various capacities can be used. A second medium is formed by injecting brown rice, 0.05-1 g of ginseng and 7-11 g of purified water per 10 g of brown rice. At this time, it is preferable that a plurality of glass bottles (1) are stored in a storage box (B) as shown in FIG. 2, and then a fixed amount is automatically injected at one time using a brown rice injector or a water injector.

The sealing step (S30) is a step of covering the inlet of the glass bottle (1) with a lid after sealing the medium and sealing it. As shown in FIG. 4 (a), a cotton ball 2 is used as a lid, and the cotton ball can prevent foreign substances from being introduced into the balloon and allow external heat or moisture to enter and exit. Here, it is preferable that the cotton wool 2 is made of a cotton wool which is not a cloud wool so that cotton dust is blown into the inside of the glass bottle or an external foreign matter is prevented from flowing into the glass bottle.

The sterilization step (S40) is a process of putting a glass bottle into a sterilizer and sterilizing it. When sterilized in a high pressure sterilizer, it is sterilized at 100 to 150 ° C for 1 hour or sterilized at 100 to 150 ° C for 5 to 6 hours to sterilize harmful microorganisms in the secondary culture medium and soften the medium. I can do it.

The cooling step (S50) is a step of transferring the glass bottle to a cooling chamber and cooling the glass bottle at a temperature of 10 to 20 DEG C for 10 to 20 hours. Preferably, the glass bottle is cooled at 15 DEG C for about 15 hours.

The seeding step (S60) is a step of inoculating the insecticide liquid seedlings into a secondary culture medium in a glass bottle after opening the lid in a clean bench of a clean room, and the secondary culture inoculated in FIG. 3C is shown.

The secondary culture medium facilitates formation of fruiting body by allowing moisture in the culture medium and ventilation to be maintained for a considerable period of time, facilitating the activation of mycelium and providing a space between the brown rice and the brown rice, When 1 g is contained, 7 to 11 g of purified water is injected to prevent the air gap from being excessively increased to reduce the gap in the culture medium, to prevent the ventilation from being properly maintained, or to prevent the mycelial adhesion from adversely affecting the mycelial growth.

In the culturing step (S70), the inlet of the glass bottle is covered with a cover, and the culture is transferred to an incubation room made of a dark room, and the incubation is carried out for 5 to 10 days at a temperature of 22 to 26 DEG C. As described above, when the incubation is carried out, the mycelium is not irradiated with light because it is not irradiated inside, and the mycelium is spread evenly over the whole area of the secondary culture without growing up, and the mycelial tissue becomes dense, When light is illuminated in step S80, fruiting bodies are uniformly raised and spread simultaneously over the entire area of the secondary culture medium.

4 (b), the lid 3 is provided with a through hole 4 on the upper surface thereof and a cap 5 for opening and closing the through hole 3 by rotation on the upper side, And a graduation is formed around the through-hole 4.

Here, the plug 5 is provided with a plate-shaped main body 5a and a handle 5b protruding from the top of the main body. Therefore, it is possible to open and close the stopper 5 and adjust the degree of opening and closing of the stopper 5, thereby providing optimum ventilation, temperature and humidity conditions necessary for the growth of the Chinese caterpillar fungus. In this case, the mesh 4a is installed in the through-hole 4 so as to ventilate and ventilate, and to block foreign matter and sickness during cultivation and cultivation. The stopper 5 is connected to the lower portion of the rotary shaft 5c and the rotary shaft 5c is connected to a hole formed at the center of the cover 3 so as to be rotatable about the rotary shaft 5c.

The thus-inoculated cordyceps specimens pass through the surface of the secondary culture medium to penetrate into the interior of the secondary culture medium after the passage of the culture day, and the surface of the secondary culture medium (S) It takes about a week or so to fill up.

The cultivation step (S80) is a step of cultivating Cordyceps (T) by growing Cordyceps sinensis for 30 days to 50 days while maintaining the temperature at a relatively low temperature of 15 to 21 DEG C and an optimum humidity of 60 to 70% . When the growth conditions of the cultivation room exceeds 21 ℃ or 70% of humidity, the mycelial growth of mycelium becomes active rather than the formation of fruiting body, which inhibits the growth of fruit body. In addition, it is preferable that the cultivation step maintains the brightness of the artificial illumination light at 1,000 to 2,000 lux.

As described above, since the optimum condition necessary for growth of Cordyceps, including lightness, temperature, and humidity, is provided, it is possible to produce Cordyceps, which is excellent in foot and growth of Cordyceps, uniformly produces Cordyceps, You can do it.

In the culturing step S70, the surface of the hypha initially having a light beige color gradually grows in color and starts the cultivation step (S80) if it becomes dark orange or orange. In the cultivation step (S80), as shown in Fig. 5A, a rhyolitic hyphae having a dark orange or orange color begins to be formed. This is because the growth of the spindle starts to be formed, And it takes about 50 days to form a full-shaped fruiting body T as shown in Figs. 6 and 7, as shown in Fig.

In addition, in the cultivation step (S80), the temperature of the cultivation room is raised to 22 to 26 DEG C during cultivation, the head part of the fruiting body is made into mycelial state for 5 to 10 days, and then the temperature of the cultivation room is again raised to 15 to 21 DEG C (S81) for forming a plurality of branches on the body (T) by continuing to grow down to the temperature.

That is, when the fruit body (T) is grown to 3 cm or more during the growing step (S80), it is raised to a temperature of 22 to 26 degrees and cultured for 5 to 10 days to make the head part of the fruit body (T) white, And the cultivation is continued to form a plurality of branches.

At the same time, the cultivation step S80 may be performed plural times during the cultivation to form a plurality of branches at various height portions of the body.

In addition, the syringe injecting step (S90) is a step of removing the cover, injecting alcohol into the glass bottle, sealing the vessel, and aging the vessel for a predetermined period of time, thereby finally producing the Chinese cabbage. Since the secondary medium containing brown rice and ginseng is included in the wine together with the fruit body of Chinese caterpillar fungus, it is possible to enjoy the unique flavor and flavor of brown rice and ginseng.

In addition, the conventional cordyceps mushroom can be contaminated by transferring fruiting bodies into a plastic bottle after collecting only the part of the mushroom feces and transferring it to a new bottle. However, in the present invention, , It is made by injecting alcohol in a state in which a complete form of fruiting body is formed. Therefore, it is possible to include as it is uncontaminated cultivated caterpillar fungus, and it is easy to cultivate and produce funguscotchus liquor.

As a result, the method of the present invention for producing the Cordyceps sinensis is easy to cultivate and produce the Cordyceps, and the unique flavor and flavor of the brown rice and the ginseng And it is possible to control the brightness of light and maintain the temperature and humidity appropriately by optimizing the moisture and nutrients in the medium so as to have the optimum conditions for the growth, So that it is possible to produce the sake using the Cordyceps, which is excellent in the foot and growth of the Cordyceps, uniformly produces the Cordyceps, and has a strong meat quality.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the invention is not limited to the disclosed exemplary embodiments. It will be apparent to those skilled in the art that various changes and modifications may be made without departing from the spirit and scope of the invention.

1. Glass bottles
2. Sommoongchi
B. Storage box
3. Cover
4. Throughout
4a. Mesh network
5. Plug
5a. main body
5b. handle
5c. Rotating shaft
S. Secondary medium
T. Fruiting body

Claims (6)

Preparing a primary medium as a liquid seed medium, sterilizing the seed medium at 100 to 150 ° C for 20 to 30 minutes, cooling the seed medium, inoculating the seed medium into a primary culture medium, and culturing the seed medium to prepare a seedling of Cordyceps;
A medium injection step of transferring a plurality of glass bottles stored in a storage box to an automatic injector to automatically quantitatively inject brown rice, 0.05-1 g of ginseng and 7-11 g of purified water per 10 g of brown rice to form a secondary culture medium;
A sealing step of covering the entrance of the glass bottle with a cover made of a bundle of cotton sausage and sealing it;
A sterilization step in which a glass bottle is placed in a sterilizer and sterilized at 100 to 150 ° C;
A cooling step in which the glass bottle is transferred to a cooling chamber and cooled at a temperature of 10 to 20 DEG C for 10 to 20 hours;
An inoculation step of inoculating the insecticide liquid seedlings into a secondary culture medium inside the glass bottle after opening the cover on a clean bench of a clean room;
Culturing the mycelium of Cordyceps sinensis for 5 to 10 days at a temperature of 22 to 26 占 폚 by transferring the entrance of the glass bottle to the culture room made of the dark room by covering again with the cover;
When the surface of the hyphae that initially become beige in the culturing step becomes gradually darker and becomes orange or orange, the cultivation step is carried out. In the cultivation room, a temperature of 15 to 21 캜, a humidity of 60 to 70% A cultivation step of growing fruit bodies by growing the Cordyceps sinensis for 30 days to 50 days while maintaining the brightness of light of 2,000 lux; And
A lyophilization step in which the lid is removed and a glass bottle in which a fruiting body is formed is injected with alcohol and then sealed and aged so that the secondary culture medium containing brown rice and ginseng is directly included in the wine together with the fruiting body of the Chinese caterpillar fungus,
The primary culture medium is prepared by adding 10 to 20 g of sugar, 10 to 20 g of malt extract, 10 to 20 g of dextrose, 3 to 7 g of yeast, 3 to 7 g of peptone, 3 to 7 g of yeast extract, 1 to 5 g of arginine, 0.01 to 0.1 g of niacin, 0.01 to 0.1 g of niacin, 0.01 to 0.1 g of ascorbic acid, 0.01 to 0.1 g of NaCl, 0.01 to 0.1 g of FeCl _3, 0.01 to 0.1 g of K ₂ HPO ₄ , 0.01 to 0.1 g of MgSO _{4, and} 0.01 to 0.1 g of CaCl ₂ ,
In the cultivation step, when the fruit body has grown to a height of 3 cm or more during cultivation, the temperature of the cultivation room is raised to a temperature of 22 to 26 ° C, the head portion of the fruit body is turned white for 5 to 10 days, Forming a plurality of branches in the body by reducing the temperature to a temperature of 15 to 21 DEG C and continuing to grow a plurality of branches in the body, Wherein the method comprises the steps of:
The method according to claim 1,
Wherein the lid covering the incubation step has a through hole provided with a mesh net on an upper surface thereof, a scale is formed around the through hole, and a cap for opening and closing the through hole is provided by rotation,
The cap includes a plate-like body, a handle protruding from the upper portion of the body, and a rotation shaft connected to a lower portion of the body and connected to a hole formed at the center of the cover, And the degree of opening and closing of the opening can be controlled.
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