KR101753304B1 - A cosmetic composition for skin whitening - Google Patents

Abstract

The present invention relates to a cosmetic composition for whitening skin, which comprises a mixture of raw persimmon, Chinese cabbage, golden and white mixed extract.
The cosmetic composition according to the present invention uses a safe natural preparation and has no side effects when applied to skin. It has an excellent effect on skin whitening through inhibition of tyrosinase activity and inhibition of melanin biosynthesis, and is suitable as a skin whitening cosmetic composition.

Description

[0001] The present invention relates to a cosmetic composition for skin whitening,

The present invention relates to a cosmetic composition for skin whitening comprising a herbal mixed extract.

Several factors are involved in determining the skin color of a person. Among them, factors such as the activity of melanocytes that make melanin pigment, the distribution of blood vessels, the thickness of skin, and the presence or absence of pigment in and out of human body such as carotenoid and bilirubin Are important.

The formation of melanin pigment affects genetic factors, hormonal secretion, physiological factors such as stress, and environmental factors such as ultraviolet irradiation.

The melanin pigment produced in melanocytes of body skin is a phenolic polymer substance having a complex form of black pigment and protein. It protects the subcutaneous skin organs by blocking ultraviolet rays irradiated from the sun, and at the same time, free radicals And protects proteins and genes in the skin.

Melanin, which is produced by stress stimuli inside and outside the skin, is a stable substance that does not disappear until the skin is excreted through skin keratinization even if the stress disappears. However, when melanin is produced more than necessary, it induces hypercholesterolemia such as spots, freckles, and dots, resulting in poor cosmetic results.

In response to such demands, substances having ascorbic acid, kojic acid, arbutin, hydroquinone, glutathione, derivatives thereof, or tyrosinase inhibiting activity have been conventionally used in cosmetics or pharmaceuticals, The use thereof is limited due to a whitening effect, a safety problem to the skin, a problem of formulation and stability in combination with cosmetics, and the like. Accordingly, it is urgently required to develop a composition for skin whitening that is safer and more effective for a living body than a conventional skin whitening composition.

KR10-2013-0125878 2013.11.20 KR10-2013-0090169 2013.08.13

It is an object of the present invention to provide a cosmetic composition having a whitening effect.

The inventors of the present invention have made intensive studies to develop a cosmetic composition having a whitening effect, and as a result, found that the raw whitish, gugija, golden and white papillae have excellent skin whitening effect and completed the present invention.

The present invention provides a mixed extract of green beans, bean curd, golden and white beans.

According to the synergistic effect of mixing the four kinds of medicinal materials, the mixed extract of Lilium persimmon, Gejia, Gugija, Golden and White ground of the present invention has cytotoxicity lower than that of each medicinal material and has excellent skin whitening effect.

In the present invention, saengjihwang (Rehmanniae Radix Recens) is a perennial plant belonging to the family Scrophulariaceae is Rehmannia (Rehmannia glutinosa (Gaertner) Liboschitz ex Steudel), and is native to Korea, China and Japan.

In the present invention, Lycium Fruit is a creeper deciduous shrub, Lycium chinense Miller) or Lycium barbarum Linne. Mature fruits are picked and dried in a cool place to make the outer skin soft and crumpled. It grows in Korea, China, and Japan.

In the present invention, the golden (Scutellaria Root) is a perennial plant belonging to the family Lamiaceae, Scutellaria baicalensis Georgi). It is distributed in Korea, China, Mongolia and eastern Siberia.

In the present invention, the blank (Angelica Root Dahurica) is a dispersion type and belonging to one years or two years sex herbal guritdae (Angelica dahurica Bentham et Hooker f. ) Or anti-blank (Angelica dahurica Bentham et Hooker f. there is. formosana Shan et Yuan). It is distributed in Korea, China and Japan.

The medicaments can be obtained at home or abroad at low cost, and can be purchased or directly collected.

In the present invention, the mixed extracts of Bokbujang, Gugija, Golden and White Bark may be the extracts obtained by mixing these extracts first, or the mixed extracts obtained by extracting each of these extracts. Preferably, the mixed extracts of Bokbujang, Use the extracted extract after mixing gold and white paper. In the present invention, the mixed extract may be in a concentrated liquid form or in a lyophilized powder form.

The mixed extract of the present invention contains 40 to 50% by weight of raw persimmon, 15 to 25% by weight of ginger, 15 to 25% by weight of gold and 10 to 10% by weight of white ginger, Preferably from 20% to 40% by weight, preferably from 40% to 50% by weight of raw persimmon, from 17% by weight to 25% by weight, from 17% by weight to 25% by weight of gold and from 15% .

The mixed extract of the present invention contains the weight percentage described above for the above four kinds of medicinal materials, and thereby exhibits remarkably excellent effect on skin whitening compared with individual extracts or two or more combination extracts. In particular, And exhibits excellent activity for skin whitening.

In the present invention, the mixed extracts of raw persimmon, Chinese goji, golden and white mixed papers can be produced by using extraction methods such as hot water extraction, immersion extraction, reflux cooling extraction and ultrasonic extraction, and the extraction times are 1 to 5 times desirable. The extraction solvent is preferably water, an alcohol having 1 to 4 carbon atoms or a mixture thereof, preferably water or an aqueous solution of ethanol, but is not limited thereto. The amount of the above-mentioned extraction solvent is 2 to 15 times the total weight of the raw persimmon, Chinese cabbage, golden and white paper. In the case of the hydrothermal (water) extract, it may be repeated one to four times at 100 to 110 ° C for 2 to 24 hours, preferably for 2 to 10 hours by water-bath reflux extraction.

In the present invention, there is provided a fragrant extract of Leaf kiwifruit, Gugija, Golden and Bleach extract obtained by further separating the mixed extracts of Lilium, Liliaceae, Golden and White. Fractionation is carried out by separation methods known in the art. Preferably, the raw persimmon, Chinese cabbage, golden and white papers are suspended in water and extracted with a solvent such as hexane, chloroform, ethyl acetate, butanol or the like to obtain extracts of raw persimmon, Chinese goat, golden and white paper.

The present invention provides a cosmetic composition for skin whitening comprising a mixture of raw persimmon, Chinese cabbage, golden and white mixed extracts.

The present invention relates to a cosmetic composition for whitening skin, which has an excellent effect on skin whitening through inhibition of tyrosinase activity and inhibition of melanin biosynthesis, Suitable.

In particular, the cosmetic composition for skin whitening comprising the combination of the extract of Leekyangguk, Gejigae, Golden, and White Bland of the present invention, when compared with each of the single extracts and the combination extract of two or more thereof, And thus has a superior skin whitening effect.

In the present invention, the mixed extract of green onion, garlic, golden and white can be added to the cosmetic in an amount of 0.001% to 10% by weight in powder or liquid form relative to the total weight of the cosmetic composition, May be added to the cosmetic in an amount of 0.01 to 5% by weight in powder form or in liquid form.

The components contained in the cosmetic composition according to the present invention include components commonly used in cosmetic compositions in addition to the raw extracts of Ganoderma lucidum, Gugija, golden and white mixed extracts as active ingredients, and examples thereof include antioxidants, stabilizers, solubilizers, vitamins, And customary adjuvants such as perfumes, and carriers.

The cosmetic composition of the present invention can be prepared into any of the formulations conventionally produced in the art and can be used as a solution, a suspension, an emulsion, a paste, a gel, a cream, a lotion, a powder, a soap, , Oil, powder foundation, emulsion foundation, wax foundation and spray, but is not limited thereto. More specifically, it can be manufactured in the form of a soft lotion, a nutritional lotion, a nutritional cream, a massage cream, an essence, a pack, an eye cream, a cleansing cream, a cleansing foam, a cleansing water, a pack, a spray or a powder.

When the formulation of the present invention is a paste, cream or gel, an animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as the carrier component .

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. In the case of a spray, in particular, / Propane or dimethyl ether.

When the formulation of the present invention is a solution or an emulsion, a solvent, a dissolving agent or an emulsifying agent is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, Glycerol, aliphatic esters, phenoxyethanol, triethanolamine, polyethylene glycol, beeswax, polysorbate 60, sorbitan sesquiolead, paraffin, polyoxyethylene sorbitan fatty acid esters, polyoxyethylene sorbitan fatty acid esters, Sorbitan stearate, glycerin monostearate, stearic acid, glyceryl stearate / fat-400 stearate, carboxy polymer, sitosterol, polyglyceryl 2-oleate, ceramide, cholesterol, , Macadamia oil, carboxyvinyl polymer, xanthan gum or sorbitan fatty acid Esters and the like.

When the formulations of the present invention are suspensions, the carrier component may be a suspending agent such as water, diluents such as ethanol, butylene glycol or propylene glycol, ethoxylated isostearyl alcohol, polyoxyethylene sorbitol esters and polyoxyethylene sorbitan esters Microcrystalline cellulose, hydroxyethylcellulose, sodium hyaruronate, phenoxyethanol, aluminum metahydroxide, bentonite, agar or tracant, etc. may be used.

When the formulation of the present invention is an interfacial active agent-containing cleansing, the carrier component may include aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters.

The present invention relates to a cosmetic composition for whitening skin, which has an excellent effect on skin whitening through inhibition of tyrosinase activity and inhibition of melanin biosynthesis, It has excellent effect.

FIG. 1 is a view showing changes in cell survival rate according to the treatment with mixed extract of Leaf spp., Gugija, Golden and White spots.
FIG. 2 is a view showing the inhibition of tyrosinase activity by treatment with raw leaves, gingko leaves, golden and white mixed extracts.
FIG. 3 is a graph showing the inhibition of melanin formation by treatment with raw leaves, ginger, golden and white mixed extracts.
FIG. 4 is a graph showing changes in melanin production according to the treatment of raw medicinal herbs, gold, white papers or goji medicinal herb extracts.

Hereinafter, the present invention will be described in more detail with reference to the following examples and experimental examples. However, the following examples should not be construed as limiting the scope of the present invention, but should be construed to facilitate understanding of the present invention.

Example 1 Preparation of Mixed Extract of Leaf Spot, Gugija, Golden and White

(Origin: Jeongbuk Mountain), gold (origin Jeonnam Mountain), and white ground (origin Jeongbuk Mountain) were mixed with omni It was purchased from the herb, washed with water and used after shading.

100 g of raw persimmon, 50 g of ginger, 50 g of gold and 35 g of white gum were placed in 1 L of distilled water, refluxed at 100 ° C. for 4 hours and concentrated under reduced pressure to obtain 62.26 g of raw persimmon, White fly mixed extracts were prepared.

As a control, 100 g of raw medicinal herbs, gold, white paper or goji medicine were added to 1 L of distilled water, refluxed at 100 ° C. for 4 hours, and concentrated under reduced pressure to obtain 41 g of raw persimmon, 34 g 31 g of white paper was prepared.

Example 2. Confirmation of cytotoxicity of mixed extracts of Leaf spore, Gugija, Golden and White spots

MTT assay was used to determine the cytotoxicity of the mixed extracts of Baiji, Golden, White and Gugija.

B16F10 melanoma cells from mice were seeded in a 24-well plate at a density of 10 ⁴ cells / well, and then incubated with 10% fetal bovine serum, 2 mM glutamine, 100 U / mL penicillin, and 100 mg / mL streptomycin And cultured in DMEM media at 37 ° C in a 5% CO ₂ incubator for 24 hours. The cultured cells were treated with a mixture of fresh persimmon, golden, white and gugija extracts (0 ~ 400 ㎍ / mL). After further incubation for 48 hours pH and then washed twice with PBS in 7.4 to 10 times in the culture liquid diluted MTT reagent (0.5 mg / mL) with 1 mL treated with 37 ℃ for the next 4 hours, and the reaction in a 5% CO ₂ cell incubator . After 4 hours of MTT reaction, the culture solution was removed, and 1 mL of DMSO solution was added to dissolve the cells. Then, the concentration of forma- tat salt was measured using a spectrophotometer at 540 nm.

The cell viability was calculated by the following formula 1, and the results are shown in FIG.

[Formula 1]

The experimental results are shown in Fig. As shown in FIG. 1, the effect of the mixed extract of green onion, golden, white and gugija on cell survival was not significant until about 100 ug / ml, but when the cell concentration was 200 ㎍ / mL or more And the cytotoxicity was significantly higher than that of the control group.

Example  3. Mixture of raw leaves, golden, white and goji mixed extract Tyrosinase  Confirm inhibitory activity

Tyrosinase activity of B16F10 melanoma cells derived from mouse was investigated.

The cells were cultured in a 100 mm cell culture dish until melanoma cells were 95% confluent. The cultured cells were washed twice with PBS (pH 7.4), and the enzyme was extracted with 0.1 M phosphate buffer (pH 6.8) containing 0.1% triton 100. The protein concentration of the enzyme solution was quantitated to 20 ㎍, and then treated with the mixture of raw persimmon, gold, white and gugija at concentration (0 ~ 1 ㎎ / mL) and reacted at 37 ℃ for 10 minutes. The reaction solution was dispensed with 0.15% dopa (3,4-Dihydroxy-L-phenylalanine) solution, reacted at 37 ° C for 20 minutes, and then absorbance was measured at 475 nm using a spectrophotometer.

Since the reaction color is brown, a sample group (a) was prepared by adding distilled water instead of 0.15% dopa solution. A sample group (a) prepared by reacting with 0.15% dopa solution and bamboo, golden, After excluding a), the value was calculated.

Tyrosinase activity inhibition was calculated by the following formula 2, and the results are shown in FIG. 2 below.

[Formula 2]

The experimental results are shown in Fig. As shown in Fig. 2, it was decreased from 0.1 ㎎ / mL in a concentration-dependent manner in comparison with the control group due to the prescription of raw bamboo, gold, white paper and gujie, and the tyrosinase activity was decreased to 12.1 and 33.1% at 0.1 and 1 ㎎ / mL, respectively .

Example  4. Confirmation of inhibition of melanin formation by mixed extracts of raw leaves, golden, white and goji

Melanin biosynthesis was measured by mouse derived B16F10 melanoma cells.

Using the B16F10 10% of melanoma cells in 100 mm culture dishes fetal bovine serum, 2 mM glutamine, 100 U / mL penicillin and 100 mg / mL DMEM media the streptomicin includes within 37 ℃ incubator containing 5% CO ₂ Cells were cultured until 90% confluency.

B16F10 melanoma cells were plated at 210 ⁴ cells / well in a 24-well plate and cultured in a 5% CO ₂ incubator at 37 ° C for 24 hours. The cells were treated with kojic acid (200 μM), which was used as a positive control, and extracts of each of the extracts, each of the individual medicines, the combination extracts of each of the extracts, Time. After removing the culture medium, the cells were washed twice with PBS, and 1 N NaOH was added to the wells to which the cells were attached, followed by further incubation for 30 minutes. The cultured melted cells were collected in a tube and boiling was carried out at 100 ° C for 30 minutes. Absorbance was measured at 415 nm using a spectrophotometer. The melanin concentration was calculated using the Melanin standard curve. The melanin concentration was corrected by quantifying the protein concentration of the melted liquid. The protein concentration was measured using a BCA protein assay kit (Thermo Scientific, 23225) based on the Lowry method.

The results are shown in Figs. 3 and 4. Fig.

FIG. 3 (a) is a result of treating the mixed extract of green onion yellow, golden, white and gugija prepared in Example 1, and FIG. 3 (b) Concentration in the solution. As shown in FIG. 3, it was confirmed that the melanin production was significantly decreased depending on the treatment with the mixture of raw leaves, golden, white and gugija extracts, and the melanin biosynthesis inhibitory effect of the individual medicinal preparations of the raw leaves, .

On the other hand, FIG. 4 shows the melanin biosynthesis change according to the individual extract treatment of raw leaves, golden, white or goji. As shown in Fig. 4, it was confirmed that the individual extracts of raw persimmon, gold, white paper or goji were not effective for melanin biosynthesis.

In other words, the individual extracts of Baijiwang, Golden, White or Gugija have little effect on the inhibition of melanin synthesis, but the combined extracts of Baijiwang, Golden, White and Gugija combined with each other have a synergistic effect on the inhibition of melanogenesis Had a remarkable effect.

In addition, considering the cytotoxicity and the like of each individual medicinal plant extract, it was confirmed that the combined extracts of raw persimmon, golden, white and gugija combined with the weight parts had remarkable whitening effect than any other combination.

Formulation Example  1. Production of cosmetic lotion containing bamboo leaves, gugija, golden and white mixed extracts

1 kg of cosmetic lotion (skin lotion) containing the extract of Bokwanghwang, Gugija, golden and white mixed extract (Example 1) was prepared by the content of [Table 1] shown below.

[Table 1] Composition of cosmetic lotion containing raw persimmon, Gujia, golden and white paper

Formulation Example 2. Preparation of nutrition lotion containing mixed extract of bergisil, gugija, golden and white paper

1 kg of nutritional lotion containing the raw extract of Guanxi, Guji, golden and white mixed extract (Example 1) was prepared by the content of [Table 2] shown below.

[Table 2] Composition of nutritional lotion containing mixed extract of Bean Sulfur, Gugija, Golden and White Blend

Claims (5)

The present invention relates to a method for producing a fermented soybean curd refuse comprising a mixture of raw persimmon, Chinese goose, golden, and white paper, comprising 40 to 50% by weight of raw persimmon, 15 to 25% by weight of gypsum, 15 to 25% by weight of gold and 10 to 20% A cosmetic composition for skin whitening. delete The cosmetic composition for skin whitening according to claim 1, wherein the raw leafy goat, gugija, golden and white mixed extracts are extracted with at least one solvent selected from water, an alcohol having 1 to 4 carbon atoms, or a mixture thereof. The cosmetic composition for skin whitening according to claim 1, wherein the skin whitening is caused by inhibition of tyrosinase activity, inhibition of melanin biosynthesis, or both. The cosmetic composition according to claim 1, wherein the cosmetic material has any one of formulations selected from the group consisting of a soft lotion, a nutritional lotion, a nutritional cream, a massage cream, an essence, a pack, an eye cream, a cleansing cream, a cleansing foam, a cleansing water, a pack, Cosmetic composition.

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