KR100974289B1 - Compositions for Preventing Hair Loss or Stimulating Hair Sprouting - Google Patents

Abstract

The present invention relates to a composition for preventing hair loss or promoting hair growth comprising a plant extract selected from the group consisting of Geumbulchoe extract, Giltyeong extract, Baekshou extract and Sokdan extract as an active ingredient. The composition of the present invention not only has excellent hair loss prevention and hair growth activity, but also is very safe for human body.

Golden Fire Candle, Gilgyeong, Baekshao, Fast, Hair Growth, Hair Loss, Hair Regrowth

Description

Compositions for Preventing Hair Loss or Stimulating Hair Sprouting}

The present invention relates to a composition for preventing hair loss or promoting hair growth.

Many causes and mechanisms of alopecia are unknown. The main causes are reportedly related to male hormones and steroid hormones. For example, the cause of androgenetic alopecia is androgenic hormone balance, which has been reported to be the main cause of androgenetic alopecia (androgen) (J. Shapiro et al., Hair regrowth.Therapeutic agents, Dermatol. Clin. 16 (2), 341-356, 1998). In addition, a paper presented that stress is a significant cause of alopecia. On the other hand, studies to determine the cause of baldness and hair loss, has recently been suggested that the impairment of kidney function is a direct cause of hair loss.

Attempts have been made to develop materials that can prevent hair loss or promote hair growth. However, this effort was an unfinished development that almost resulted in failure or only partially succeeded. There are several anti-hair loss or hair growth drugs that have been certified by the US Food and Drug Administration (FDA), but they do not achieve satisfactory hair loss or hair growth.

Republic of Korea Patent No. 0382602, Chlorella 6.8-19% by weight, yeast 4.1-13% by weight, white-nap 3.3-13% by weight and pollen 4.6-19% by weight, eggplant 1.8-6% by weight, bamboo salt 0.02-0.32 weight % Or 0.02 to 2.3% by weight of cow bone powder, and 1.8 to 2.2% by weight as a herbal medicine, 1.8 to 14% by weight, about 2.4 to 4.6% by weight of lactose, 2.4 to 3.2% by weight of white porcelain, and 2.4 to 3.2% by weight of small fruit. %, 2.7-14% by weight, 2.7-6% by weight, 2.7-6% by weight, 2.7-3.1% by weight, yeonyeoncho 2.7-3.1% by weight, 2.7-3.1% by weight, Xinhua 2.7-3.1%, 5.4-6.0% by weight, charcoal 2.7-3.1 Wt.%, 1.8 ~ 2.2% by weight, 4.1 ~ 4.5% by weight, 4.1 ~ 4.5% by weight, 2.8 ~ 3.2% by weight of Schizandra chinensis, 4.1 ~ 4.5% by weight of Yin Yang Guk, 1.8 ~ 2.2% by weight of scavenger, 2.7-3.1 Astragalus It contains weight%, thick 2.7-3.1 weight% or health 2.7-3.1 weight%, 0.8-2.2 weight% seaweed ash, 0.02-1.2 weight% zinc sulfate and 0.02-0.7 magnesium oxide as electrolyte It discloses an anti-hair loss, containing% by weight and the hair growth promoting composition.

US Patent Publication No. 2005-260152 discloses that the garlic component dissolved in a liquid carrier including water, polysorbate and auxiliary ingredients exerts a hair growth action. US Patent Publication No. 2005-239697 discloses a composition for promoting hair growth comprising an actin-binding peptide as an active ingredient.

WO 2005-063279 discloses that IL (interleukin) -15 is associated with promoting hair growth or preventing hair loss. WO 2005-053763 discloses a method for promoting hair growth comprising transplanting the dermal papilla or cultured dermal papilla cells into the skin.

Numerous papers and patent documents are referenced and cited throughout this specification. The disclosures of the cited papers and patent documents are incorporated herein by reference in their entirety to better understand the state of the art to which the present invention pertains and the content of the present invention.

The present inventors screened thousands of natural plant extracts or fractions in order to develop novel substances that can prevent hair loss or promote hair growth. The present invention was completed by confirming that it can be usefully used for promoting hair growth.

Accordingly, it is an object of the present invention to provide a composition for preventing hair loss or promoting hair growth.

Other objects and advantages of the present invention will become more apparent from the following detailed description of the invention, claims and drawings.

According to an aspect of the present invention, the present invention provides a composition for preventing hair loss or promoting hair growth comprising a plant extract selected from the group consisting of Geumbulcho extract, Gilyeong extract, Baekshou extract and Sokdan extract as an active ingredient.

The present inventors screened thousands of natural plant extracts or fractions in order to develop novel substances that can prevent hair loss or promote hair growth. It was confirmed that it can be usefully used for promoting hair growth.

Geumbulcho used as an active ingredient of the present invention is a perennial grass of Asteraceae, scientific name is Inula japonica . In oriental medicine, it is used as urine medicine, cough sputum medicine, dry stomach medicine, hemostatic medicine and inflammatory medicine.

Gilyeong ( Platycodon grandiflorum ) used in the present invention is the root of the perennial herbaceous bellflower belonging to Campanulaceae, the main components are Platicodione-A, C, D1 and D2, Platicodigenin, polygalaxin-D1 And about 10 kinds of saponin glycosides such as D2 and platicodine, and other ingredients such as betulin, inutin, and phytosterol.

In addition, Baek Shou ( Cynanchum) used in the present invention wilfordii ) is a perennial herb that belongs to the Polygonaceae, its height is 1-3 m, and its root is thick as a tuber. The main constituents of Baek Shao Shou are Sinan Cotoxin and Phytotocotoxin, which are used as tonics and tonics.

In addition, Phlomis used in the present invention ( Phlomis umbrosa ) is a perennial herb belonging to Lamitae (Labiatae), its height is 70-150 cm, and has about 5 large tubers. The leaves are used for food and the roots and rhizomes are used for medicinal purposes.

The Geumbulcho extract, Gilgyeong extract, Baishao extract and Sokcho extract are various extraction solvents, such as (a) water, (b) anhydrous or hydrous lower alcohols having 1 to 4 carbon atoms (methanol, ethanol, propanol, butanol, etc.) (c) a mixed solvent of the lower alcohol and water, (d) acetone, (e) ethyl acetate, (f) chloroform, (g) 1,3-butylene glycol, and (h) butyl acetate as extraction solvents. Can be obtained. Most preferably, the extract of the present invention is obtained using water as the extraction solvent. On the other hand, it will be apparent to those skilled in the art that the extract of the present invention can be obtained in addition to the above-described extraction solvents, extracts having substantially the same effect using other extraction solvents.

In addition, the extract of the present invention includes an extract by the aforementioned extraction solvent. Extracts of the present invention may be prepared in powder form by additional processes such as distillation under reduced pressure and freeze drying or spray drying.

According to a preferred embodiment of the present invention, the Geumbulcho extract, Gilkyung extract, Baekshou extract or Sokcho extract as active ingredients in the composition of the present invention is 0.10-80% by weight relative to the total weight of the composition.

According to a preferred embodiment of the present invention, the composition of the present invention is made of a cosmetic composition or a food composition.

The components included in the cosmetic composition of the present invention include components commonly used in cosmetic compositions in addition to Geumbulcho extract, Gilkyung extract, Baek sewage extract and Sokdan extract as active ingredients, and include, for example, antioxidants, stabilizers, solubilizers, vitamins, Conventional adjuvants such as pigments and perfumes, and carriers.

The composition for improving skin condition of the present invention may be prepared in any formulation commonly prepared in the art, for example, solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, surfactants -Can be formulated with, but not limited to, cleansing, oils, powder foundations, emulsion foundations, wax foundations and sprays. More specifically, it can be manufactured in the form of a soft lotion, a nutritional lotion, a nutritional cream, a massage cream, an essence, an eye cream, a cleansing cream, a cleansing foam, a cleansing water, a pack, a spray or a powder.

When the formulation of the present invention is a paste, cream or gel, animal oils, vegetable oils, waxes, paraffins, starches, trachants, cellulose derivatives, polyethylene glycols, silicones, bentonites, silicas, talc or zinc oxide may be used as carrier components. Can be.

In the case where the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. Especially, in the case of a spray, a mixture of chlorofluorohydrocarbons, propane / Propane or dimethyl ether.

When the formulation of the present invention is a solution or an emulsion, a solvent, a dissolving agent or an emulsifying agent is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, , 3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan fatty acid esters.

When the formulation of the present invention is a suspension, liquid carrier diluents such as water, ethanol or propylene glycol, suspending agents such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, microcrystals Soluble cellulose, aluminum metahydroxy, bentonite, agar or tracant and the like can be used.

When the formulation of the present invention is a surfactant-containing cleansing, the carrier component is an aliphatic alcohol sulfate, an aliphatic alcohol ether sulfate, a sulfosuccinic acid monoester, isethionate, an imidazolinium derivative, a methyltaurate, a sarcosinate, a fatty acid amide. Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters and the like can be used.

When the composition of the present invention is prepared as a food composition, as an active ingredient, as well as Geumbulcho extract, Gilkyung extract, Baek sewage extract, and Sokdan extract, as well as components commonly added during food production, for example, protein, carbohydrate Contains fats, nutrients, seasonings and flavorings. Examples of the above carbohydrates include monosaccharides such as glucose, fructose and the like; Disaccharides such as maltose, sucrose, oligosaccharides and the like; And sugars such as conventional sugars such as polysaccharides such as dextrin, cyclodextrin and the like and xylitol, sorbitol, erythritol. As the flavoring agent, natural flavoring agents (tauumatin, stevia extract (for example rebaudioside A, glycyrrhizin, etc.) and synthetic flavoring agents (saccharin, aspartame, etc.) can be used.

 For example, when the food composition of the present invention is prepared with a drinking agent, citric acid, liquid fructose, sugar, glucose, acetic acid, malic acid, fruit juice, tofu extract, jujube extract, licorice extract, etc. May be additionally included.

According to a preferred embodiment of the present invention, the composition of the present invention includes the Geumbulcho extract as an essential active ingredient, and further includes a Gilgyeong extract, Baekshou extract or Sokdan extract.

According to a preferred embodiment of the present invention, the composition of the present invention is at least two plant extracts, more preferably at least three plant extracts selected from the group consisting of Geumbulchocho extract, Gilkyung extract, Baekshou extract and Sokdan extract, Most preferably, all four plant extracts are included as an active ingredient.

When the composition of the present invention comprises three kinds of plant extracts, it is preferable to include a mixture of Giltyeong extract, Baek sewage extract and Sokcho extract as an active ingredient.

The composition of the present invention works very effectively in preventing hair loss, promoting hair growth or promoting hair growth. As used herein, the terms "anti-hair loss", "hair growth or hair growth promotion" is used in the same sense, which has the same meaning as another term wool and the like used in the art.

The hair loss prevention or hair growth promoting composition of the present invention promotes keratinocyte cell proliferation and ultimately plays a very good role in preventing hair loss in the living body and promoting hair growth, and is very safe for human body.

Hereinafter, the present invention will be described in more detail with reference to Examples. These examples are only for illustrating the present invention in more detail, it is to those skilled in the art that the scope of the present invention is not limited by these examples in accordance with the gist of the present invention. Will be self-evident.

Example

Example material

Geumbulcho, Gilgyeong, Baekshou and Sokdan used in the present invention were purchased from Gyeongdong market and used for extract preparation.

Example 1 Preparation of a Composition Containing Geumbulcho Extract

50 g of dried Geumbulcho root was added to 1.5 L of water and extracted for about 2 hours at a temperature higher than room temperature to obtain an aqueous extract. The extract was concentrated and dried to obtain a powder in the amount shown in Table 1 below.

division Weight (g) Extraction efficiency (%) Early 50 100 Dry powder 11.8 23.6

Example  2: Street view  Preparation of the composition comprising the extract

50 g of dried road-length was added to 1.5 L of water and extracted at a temperature of about 2 hours or longer to obtain an aqueous extract. The extract was concentrated and dried to obtain a powder in the amount shown in Table 2 below.

division Weight (g) Extraction efficiency (%) Early 50 100 Dry powder 11.5 23

Example  3: Baek Ha Shou  Preparation of the composition comprising the extract

White sewage extract was prepared in the same manner as in Example 1 using the root region of Baek sewage. The amount of the white sewage extract is shown in Table 3 below.

division Weight (g) Extraction efficiency (%) Early 50 100 Dry powder 12.1 24.2

Example  4: Preparation of a composition comprising a fast extract

1.5 g of distilled water was added to 50 g of fast-acting, and heated and extracted at 80-90 ° C. for 2 hours to obtain 0.7 L of an aqueous extract. 0.7 L of the obtained extract was stored at room temperature, and distilled water corresponding to half of the amount added during the first extraction was added to the remaining medicinal herbs, and the second extraction was heated and extracted at 80-90 ° C. for 2 hours. One liter of the extract obtained over the first and second phases was collected and heated with heat (90 ° C.) to concentrate the volume of the extract to the final 500 ml.

The extract obtained in each step was dried under reduced pressure (freeze dryer, Edwards USA) to obtain a powder, and the extraction efficiency was calculated based on the weight of the powder (see Table 4).

division Weight (g) Extraction efficiency (%) Early 50 100 Dry powder 10.5 21.0

The extraction efficiencies of the heating and extraction steps in Table 4 were slightly varied with each run and generally showed an extraction efficiency of 18-24%.

Example  5: Keratinocyte  Cell proliferation activity experiment

Human HaCaT keratinocytes (ACTT) cultured in DMEM medium containing 10% FBS (fetal bovine serum) were treated with 10 μg / ml of the above-mentioned plant extracts and incubated for 72 hours. Then, CCK-8 reagent (Dojindo Molecular Technologies) was added and reacted for 2 hours, and then absorbance was measured at 450 nm. Instead of plant extracts, the degree of proliferation of cells treated with distilled water was 100%, and the activity of the experimental group was calculated as a relative value.

extract Keratinocyte growth rate (% control) Control 100 Gold candle 156 Street view 145 Baek Ha Shou 140 Fast 138 Gilkyung, Baekshao and Fastan 169 Golden Fire, Gilgyeong, Baekshao and Sokdan 182

As can be seen in Table 5, the plant extract of the present invention greatly enhanced the cell proliferation of keratinocytes, in particular, the mixture of Geumbulcho, Gilkyung, Baekshou and Sokdan extract increased synergistically the cell proliferation of keratinocytes. .

Formulation example  One

An example of the formulation of the lotion in the cosmetic composition comprising the extract (Gumbulcho, Gilkyung, Baekshao, Sokdan extract, or a mixture of the four extracts) obtained above is as follows:

ingredient Content (% by weight) extract 1.0 1,3-butylene glycol 5.2 Oleyl alcohol 1.5 ethanol 3.2 Polysorbate 20 3.2 Benzophenone-9 2.0 Carboxyl Vinyl Polymer 1.0 glycerin 3.5 incense a very small amount antiseptic a very small amount Purified water Balance system 100

Formulation example  2

Examples of the formulation of the hair tonic in the cosmetic composition containing the extract of the present invention (Gumbulcho, Gilkyung, Baekshao, Sokcho extract, or a mixture of the four extracts) are as follows:

ingredient Content (% by weight)  extract 1.0 glycerin 3.0 ethanol 55.0 Pyrotonamine 0.1 Castor oil 5.0 Squalane 3.0 incense a very small amount Purified water Balance system 100

Example  6: hair growth efficacy analysis

The back of the 8-week-old C57BL / 6 mouse (Central Experimental Animal) was shaved with a razor blade in an area of about 2-4 cm 2 and confirmed that there was no residual hair after 24 hours. Six mice were assigned to each experimental group. The mouse hair tonic of Formulation Example 2 was applied to the shaving site three times daily, and the control group was applied to which distilled water was added instead of the plant extract. The hair growth state was observed while applying for a total of 4 weeks. The experimental results are summarized in Table 8.

extract Hair growth efficacy (% control) 1 week 2 weeks 3 weeks 4 Weeks Control 10% 24% 36% 58% Gold candle 18% 40% 58% 80% Street view 15% 38% 55% 68% Baek Ha Shou 14% 35% 52% 65% Fast 16% 38% 56% 68% Gilkyung, Baekshao and Fastan 20% 42% 60% 82% Golden Fire, Gilgyeong, Baekshao and Sokdan 22% 50% 70% 90%

As can be seen in Table 8, the plant extract of the present invention is excellent in hair growth efficacy, in particular the hair growth efficacy of the mixture of Geumbulcho, Gilgyeong, Baekshou and Sokdan extract was synergistically increased.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the same is by way of illustration and example only and is not to be construed as limiting the scope of the present invention. Thus, the substantial scope of the present invention will be defined by the appended claims and equivalents thereof.

Claims (6)

Geumbulcho ( Inula japonica ) hair loss prevention or hair growth promoting composition comprising as an active ingredient. The method of claim 1, wherein the composition comprises a Geumbulchocho extract as an essential active ingredient, and prevents hair loss further comprising Gilycodon grandiflorum extract, Cynanchum wilfordii extract or Phlomis umbrosa extract. Or a composition for promoting hair growth. delete According to claim 2, wherein the composition is anti-hair loss or hair growth promoting composition, characterized in that it comprises the Geumbulcho extract, Gil-Gyeong extract, Baek sewage extract and Sokdan extract as an active ingredient. The composition of claim 1, wherein the composition is a cosmetic composition. The composition of claim 1 wherein the composition is a food composition.