KR101673863B1 - Cosmetic composition containing polymersomes encapsulated peptides or hydrolyzed millet - Google Patents

Abstract

The present invention relates to a cosmetic composition containing a polymer or a hydrolyzed starch polymer, and more particularly to a cosmetic composition containing an anti-aging, antioxidant, whitening, elasticity, wrinkle, skin clarity improvement, moisturizing, , Or a polymer having a hydrolyzed residue captured therein, to thereby further increase the effect of the peptide component.

Palmitoyl tripeptide-5 * diaminopropionyl tripeptide-33 * peptide * hydrolyzed millet * polymer mate * anti-aging * wrinkle improvement * skin transparency improvement * skin elasticity improvement * cosmetic composition

Description

[0001] The present invention relates to a cosmetic composition containing polymers or encapsulated peptides or hydrolyzed millet,

The present invention relates to a cosmetic composition containing a polymer or a hydrolyzed starch polymer, and more particularly to a cosmetic composition containing an anti-aging, antioxidant, whitening, elasticity, wrinkle, skin clarity improvement, moisturizing, , Or a polymer having a hydrolyzed residue captured therein, to thereby further increase the effect of the peptide component.

Skin is the primary barrier of the human body and protects the internal organs of the body from external environmental influences such as changes in temperature and humidity, ultraviolet rays and pollutants, and plays an important role in the maintenance of body homeostasis such as body temperature control. This important skin, like other organs of the human body, is gradually aging as it gets older. The causes of skin aging can be divided into two major categories: internal factors such as gene mutations and tissue changes in cells, and external factors such as ultraviolet rays and humidity (Masamitsu Ichihashi, Fragrance Journal, Vol. 32, No. 5, 24-30 ). Within the skin damaged by these internal and external factors, the cell regeneration is reduced, the surrounding environment of the cell changes, the weakening of the intercellular network occurs and the skin structure is scattered. As a result, skin elasticity loss, keratinization, wrinkle formation, And the skin is atrophied.

The cause of the greatest change in aged skin is the reduction of the growth factor that was activated in young skin. Growth factors play a key role in the regeneration process of the skin. Therefore, the reduction of the growth factors in the skin causes various functional deterioration in the skin, resulting in the loss of the components in the skin and the collapse of the skin structure. In fact, the skin of young infants with the most active growth factors in the skin is easy to recover from scratches and has a remarkable regenerative ability that does not leave scars. In order to repair the wound when a wound occurs, it is a growth factor that forces the skin and blood vessel cells to gather around the wound and create new cells to produce a new skin structure together with collagen and elastin fibers. TGF-α and EGF (Epidermal Growth Factor) participate in the whole regeneration process and FGF (Fibroblast Growth Factor, vascular endothelial growth factor (VEGF), platelet-derived growth factor ( PDGF), and insulin-like growth factor (IGF).

The aging phenomenon caused by ultraviolet rays is called photoaging. In this photoaging phenomenon, active oxygen radicals are generated inside the cells by ultraviolet rays. Through the signal transmission system causing the inflammatory reaction, the active oxygen species collagen, elastin (MMP-1, MMP-3, and MMP-9, etc.) (Fisher GJ et al ., Nature, Vol. 379, 335-339) It is known that skin wrinkles are caused by reducing the elasticity of the dermal layer.

In addition, such skin aging phenomenon not only causes wrinkles on the surface of the skin but also deteriorates the skin transparency, resulting in a change in the entire surface of the skin. Recently, many studies on the transparency of the skin have been continuing. Recently, carbonated proteins are attracting attention as an index of skin damage due to external stress. Carbonated protein is a protein denaturation due to oxidative stress inside and outside of the skin. Internal factors include endogenous aging and stress. External factors include heat aging caused by the increase of skin temperature due to infrared rays, active oxygen caused by smoking Aging due to increase, and skin damage due to air pollution such as exhaust gas. Carbonized protein occurs mainly in the stratum corneum, which is the surface of the skin. It increases by the stress that the skin receives rather than endogenous aging, and it increases rapidly only in 7 days due to such stress, which causes skin aging. Carbonated proteins have been found to cause skin rashes and fine wrinkles by decreasing skin transparency, moisture retention and flexibility. The proteins of the stratum corneum are carbonized, and the stratum corneum that becomes clear and transparent becomes turbid and opaque, so that the skin tone becomes dull, the protein accumulates in the keratinocytes, and the stratum corneum turnover slows and the skin texture becomes stiff and rough. Further, as the stratum corneum is carbonized, the ability of the skin to retain its water content is lowered, and the skin glossiness is lowered. Since the accumulation of carbonized proteins in single cells such as yeast leads to a deterioration in cell proliferation, yeast has a mechanism that does not transfer carbonized proteins to daughter cells when proliferating. It is known that not only yeast but also Escherichia coli, when cleaved in an extreme environment, increases the probability of survival by driving carbonized proteins in one cell. Removing the carbonized protein, which is crucial to the skin's transparency, from the stratum corneum not only enhances skin transparency, but also increases skin moisture retention and can also increase skin luster. In addition, it has been found that declining regenerative power of skin cells with age is a decisive factor for decreasing skin elasticity.

Several studies have been carried out on the various skin-affecting components, but few techniques have been found to stabilize potent components, especially peptides. That is, the efficacious component has a low ratio of maintaining its efficacy in the cosmetic composition, and it is more difficult to exert its effect upon application to the skin. In addition, there is a case where the efficacious ingredient is difficult to effectively penetrate into the skin, and it is difficult to realize the actual efficacy. However, if such an efficacious ingredient can be stabilized in a cell-mimicked structure, the efficacy of the efficacious ingredient may not be deteriorated, and it is possible to penetrate skin cells more effectively and increase the effect on the skin.

By implementing these effects, we will be able to meet our customers' needs for the benefits we are getting from cosmetics.

Accordingly, the present inventors have been intensively engaged in research for finding and stabilizing various peptides having excellent effects on the skin, and Palmitoyl Tripeptide-5 is effective for activating skin growth factors necessary for skin regeneration , And diaminopropionoyl tripeptide-33 (33) were found to be effective for the removal of the carbaprotein. Also, it has been confirmed that various efficacious ingredients are stabilized in the polymeric complex of the skin cell mimetic, and the effect is not deteriorated in the cosmetic formulation, and the maximized effect is exerted when applying the skin.

Accordingly, it is an object of the present invention to provide a cosmetic composition which can stabilize skin-active peptide or hydrolyzed lipid components with a polymer, thereby doubling the efficacy thereof.

In order to accomplish the above object, the present invention provides a cosmetic composition comprising various peptides showing efficacy on the skin or a polymer obtained by capturing a hydrolized sugar moiety as an active ingredient.

The cosmetic composition according to the present invention is excellent in skin moisturizing effect, wrinkle improvement, skin transparency improvement, collagen synthesis and skin elasticity improvement effect by containing a polymer peptide having a capturing effect peptide or a hydrolized sugar chain, And the skin stability was also excellent.

The present invention relates to a cosmetic composition comprising, as an active ingredient, a polymer having a variety of peptides exhibiting an effect on skin or a polymer having a hydrolyzed sugar moiety.

There have been a number of peptides that have been tested to date, but it has not been easy to penetrate into the skin due to its molecular weight, size, and structure. Thus, stabilization of the peptide source using a cell-mimicking construct can enhance stabilization, penetration rate and skin effect in the formulation.

Thus, the present invention provides a method for stabilizing the peptides in a polymer to stabilize the peptides that have been tested for efficacy. Thus, the peptide capable of stabilizing the inside of the polymer jam has various effects such as anti-aging, antioxidant, whitening, elasticity, wrinkle improvement, skin texture improvement, moisturizing or irritation alleviation, -33 (Diaminopropionoyl Tripeptide-33), Palmitoyl Tripeptide-5, Palmitoyl Pentapeptide-4, Acetyl Heptapeptide-4, Copper Tripeptide-1, LYSINE POLYPEPTIDE and Acetyl Tetrapeptide-11. In addition, a variety of peptides that have proved their skin's effectiveness can be used . Efficacy peptides used in the present invention can be synthesized from peptide derivatives by synthesizing a peptide component having a desired structure through peptide bonding of a desired protein by a method well known in the art.

The diaminopropionyl tripeptide 33 used in the present invention is a peptide derivative which improves skin transparency by acting to remove the carbonized proteins produced in the horny layer of the skin by aging, stress and external harmful factors, A peptide component having a desired structure can be synthesized through peptide bonding of a desired protein.

The palmitoyl tripeptide-5 used in the present invention is effective in activating skin growth factors necessary for skin regeneration and plays a role of restoring the skin structure by restoring the environment surrounding the cells by filling the skin component lost by aging As a peptide derivative, a peptide component having a desired structure can be synthesized through peptide bonding of a desired protein by a method well known in the art.

In the present invention, the polyersome used as a carrier of the above-mentioned effective peptides is a lipid-like biocompatible biodegradable polymer that is not adsorbed to a thin film of a molecular double-layered bezachloide peptide and a polymeric peptide by self- It has an advantage that it can fundamentally block the denaturation of the efficacious substance and maintain intrinsic activity completely. The polymer jam according to the present invention is synthesized by the microfluidics technique shown in Fig. 1, and it is a state-of-the-art technology that can control the flow of fluid to develop desired size, size, and function to produce a new concept skin engineering structure. Polymers are synthesized into a double inner layer structure, which contains potential components (peptides or hydrolized residues) in the innermost layer and is surrounded by a volatile solvent such as chloroform in which the copolymer polymer is dissolved. If the solvent is removed and the polymer is cured in this state, the efficacious components having a molecular weight of 500 or more can not escape and are collected in the polyamosome. The polymer used in the preparation of the polymer according to the present invention is polylactic acid and polyethylene glycol, and it is preferable to mix the polylactic acid and the polyethylene glycol at a weight ratio of 1: 1 to 1:10 Do. The polymeric particles have a particle diameter of 300 to 1,000 nm and are synthesized to a size capable of absorbing the skin. In the present invention, the above-mentioned effector peptides can be collected alone or in combination with two or more of them.

The efficacious peptide may contain 0.0001 to 1.0% by weight based on the total weight of the polymer. If the content of the peptide is less than 0.0001% by weight, the effect of improving the skin wrinkle, elasticity and transparency of the skin can not be obtained. If the content of the peptide is more than 1.0% by weight, the effect is not increased.

The cosmetic composition according to the present invention contains the polymer-stabilized polymer in an amount of 0.00001 to 10% by weight based on the total weight of the cosmetic composition.

In addition, the cosmetic composition according to the present invention may contain a polymerase in which the hydrolyzed cap is internally captured as an active ingredient, which may be used alone or in combination with the polymerase in which the peptide is captured.

The hydrolyzed millet used in the present invention enhances skin cell activity with reduced activity in the skin to promote skin cell regeneration, thereby ultimately preventing skin aging. The hydrolyzed precursor may be extracted with water or an organic solvent by a well-known method in the art, and then synthesized as a polymer. The organic solvent used in the present invention may be at least one selected from the group consisting of ethanol, methanol, butanol, ether, ethyl acetate and chloroform, or a mixed solvent of these organic solvents and water may be used. Preferably, 80% ethanol is used.

The cosmetic composition according to the present invention may contain the hydrolyzed length in an amount of 0.00001 to 10% by weight, more preferably 0.0001 to 5% by weight, based on the total weight of the cosmetic composition. If the content of the hydrolyzed starch is less than 0.0001% by weight, improvement in skin elasticity and wrinkle can not be sufficiently obtained. If the content of hydrolyzed starch is more than 5% by weight, the increase in the effect is not significant. It is also preferable that the hydrolyzed polymer is collected in an amount of 0.001 to 10% by weight based on the total weight of the cosmetic composition.

In one embodiment of the present invention, the cosmetic composition is an anti-aging cosmetic composition having an effect of improving skin transparency and improving skin elasticity and wrinkles.

The cosmetic composition according to the present invention may be formulated containing a cosmetically or dermatologically acceptable medium or base. It may be any formulation suitable for topical application, for example, as a solution, a gel, a solid, a paste anhydrous product, an emulsion obtained by dispersing an oil phase in water, a suspension, a microemulsion, a microcapsule, In the form of a non-ionic follicle dispersing agent, or in the form of creams, skins, lotions, powders, ointments, sprays or conical sticks. It can also be used in the form of a foam or in the form of an aerosol composition further containing a compressed propellant. These compositions may be prepared according to conventional methods in the art.

The cosmetic composition according to the present invention may further comprise at least one selected from the group consisting of a fatty substance, an organic solvent, a solubilizer, a thickening agent, a gelling agent, a softening agent, an antioxidant, a suspending agent, a stabilizer, a foaming agent, a fragrance, The active ingredient may be combined with any other ingredients commonly used in cosmetics, such as ionic emulsifiers, fillers, sequestering agents, chelating agents, preservatives, vitamins, blockers, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic active agents, And may contain adjuvants commonly used in the same cosmetic or dermatological fields. Such adjuvants are introduced in amounts commonly used in the cosmetics or dermatological fields.

Hereinafter, the present invention will be described more specifically with reference to examples and test examples. It is to be understood that the scope of the present invention is not limited to these embodiments and that variations, substitutions, and insertions conventionally known in the art can be carried out, This is also included in the scope of the present invention.

[Example 1] Production of a polymer obtained by capturing palmitoyl tripeptide-5

Polylactic acid and polyethylene glycol were mixed and dissolved in chloroform at a weight ratio of 5: 2, and 1% by weight of palmitoyl tripeptide-5 was added to the total weight of the reaction product. Then, polymer jams were synthesized through microfluidics technology, Was removed and cured to synthesize the palmytoil tripeptide-5-captured polymer.

[Example 2] Production of a polymer obtained by capturing diaminopropionyl tripeptide-33

Was prepared in the same manner as in Example 1, except that the same amount of diaminopropionyl tripeptide-33 was used instead of palmitoyl tripeptide-5 in Example 1 above.

[Example 3] Production of a polymer having a hydrolyzed capped moiety

Was prepared in the same manner as in Example 1, except that the same amount of hydrolyzed precursor was used instead of palmitoyl tripeptide-5 in Example 1 above.

[Formulation Examples 1 to 4 and Comparative Formulation Examples 1 to 4]

Creams of Formulation Examples 1 to 4 and Comparative Formulation Examples 1 to 4 were prepared according to the compositions shown in Tables 1 and 2 below (unit: wt%).

Compounding ingredient Formulation Example 1 Formulation Example 2 Formulation Example 3 Formulation Example 4 Number
Prize
castle
minute Purified water Balance Balance Balance Balance The peptide polymer of Example 1 0.01 - - - The peptide polymer of Example 2 - 0.01 0.01 - The polymeric emulsion of Example 3 - - 0.01 0.01 EDTA-2Na 0.02 0.02 0.02 0.02 glycerin 5.00 5.00 5.00 5.00 Butylene glycol 5.00 5.00 5.00 5.00 U
Prize
castle
minute Vegetable hydrogenated oil 1.50 1.50 1.50 1.50 Stearic acid 0.60 0.60 0.60 0.60 Glyceryl stearate 1.00 1.00 1.00 1.00 Cetearyl alcohol 2.00 2.00 2.00 2.00 Arachidyl / behenyl alcohol &
Arachidyl glucoside 1.00 1.00 1.00 1.00 Cetylaryl Alcohol and Cetearyl Glucoside 2.00 2.00 2.00 2.00 Liquid paraffin 6.00 6.00 6.00 6.00 Caprylic / Carrick Triglyceride 6.00 6.00 6.00 6.00 Carbomer 0.05 0.05 0.05 0.05 Triethanolamine 0.05 0.05 0.05 0.05 Preservative, fragrance, pigment Suitable amount Suitable amount Suitable amount Suitable amount

Compounding ingredient Comparative Formulation Example 1 Comparative Formulation Example 2 Comparative Formulation Example 3 Comparative Formulation Example 4 Number
Prize
castle
minute Purified water Balance Balance Balance Balance Palmitoyl tripeptide-5 - 0.0001 - - Diaminopropionyl tripeptide-33 - - 0.0001 - Hydrolyzed captain - - - 0.0001 EDTA-2Na 0.02 0.02 0.02 0.02 glycerin 5.00 5.00 5.00 5.00 Butylene glycol 5.00 5.00 5.00 5.00 U
Prize
castle
minute Vegetable hydrogenated oil 1.50 1.50 1.50 1.50 Stearic acid 0.60 0.60 0.60 0.60 Glyceryl stearate 1.00 1.00 1.00 1.00 Cetearyl alcohol 2.00 2.00 2.00 2.00 Arachidyl / behenyl alcohol &
Arachidyl glucoside 1.00 1.00 1.00 1.00 Cetylaryl Alcohol and Cetearyl Glucoside 2.00 2.00 2.00 2.00 Liquid paraffin 6.00 6.00 6.00 6.00 Caprylic / Carrick Triglyceride 6.00 6.00 6.00 6.00 Carbomer 0.05 0.05 0.05 0.05 Triethanolamine 0.05 0.05 0.05 0.05 Preservative, fragrance, pigment Suitable amount Suitable amount Suitable amount Suitable amount

<Manufacturing Method of Formulation Examples 1 to 3 and Comparative Examples 1 to 5>

(1) The aqueous phase components were uniformly mixed at room temperature and heated to 70 to 75 캜.

(2) The oil components were uniformly dissolved and mixed by heating at 75 to 80 캜.

(3) The above-mentioned (1) was added to the above-mentioned (2) under agitation to homogeneously emulsify.

(4) Carbomer was added with stirring at 1% in water and neutralized with triethanolamine.

(5) After stirring, preservatives, fragrance and pigment were added.

[Test Example 1] Evaluation of moisturizing effect

The cosmetic composition of Formulation Examples 1 to 4 and Comparative Formulation Examples 1 to 4 was applied twice daily to the facial skin for 4 weeks to 25 adults who were thought to be dry or dry skin. The moisture content of the skin was measured by a corneometer at constant temperature and humidity (24 ° C, 40% relative humidity) at 2 weeks and 4 weeks after application, and the results were compared. 2.

<Connio meter measurement method>

Principle: The amount of water present in the epidermis of the skin is measured by measuring the degree of ion of water using a sensor, and the water content is calculated by calculating the amount of water. The concrete procedure is as follows.

1) A probe of the coneometer was placed on the skin area to be measured.

2) When the probe is pressed on the skin, the electric conductivity of the skin is quantified through the sensor and displayed on the screen.

3) Measurement was repeated with different measurement sites.

4) After the measurement, the sensor was wiped with a tissue paper such as a kim wipe and then measured again.

As can be seen in FIG. 2, the compositions (Formulations 1 to 4) containing the palmytoyl tripeptide-5, diaminopropionyl tripeptide-33, or the polymerase with the hydrolyzed caprine moieties , The skin moisture content was measured to be high after 2 weeks and 4 weeks of application as compared with Comparative Formulation Example 1 which did not contain the active ingredient. In particular, it was found that the composition containing Diaminopropionyl tripeptide-33 and the hydrolyzed starch polymer at the same time (formulation example 3) had the highest water content.

Accordingly, it was confirmed that the cosmetic composition according to the present invention effectively retains moisture of the skin after application and is excellent in moisture retention.

[Test Example 2] Wrinkle improving effect

In order to examine the wrinkle-reducing effect of Formulation Examples 1 to 4 and Comparative Formulation Examples 1 to 4, 140 women in their 30s were divided into 7 groups each consisting of 20 individuals. Formulation examples 1 to 4, After applying the creams of Comparative Formulation Examples 1 to 4 respectively, a replica of a silicone material was prepared, and the state of the wrinkles at the designated region was measured with a visiometer (SV60, Courage + Khazaka electronic GmbH, Germany ). The results are shown in Table 3 below. This result is the average of the parameter values after 8 weeks minus the parameter values 8 weeks before. That is, the negative value indicates that the wrinkle improving effect is higher.

division R1 R2 R3 R4 R5 Formulation Example 1 -0.28 -0.20 -0.12 -0.09 -0.07 Formulation Example 2 -0.25 -0.18 -0.09 -0.07 -0.06 Formulation Example 3 -0.30 -0.22 -0.12 -0.12 -0.10 Formulation Example 4 -0.25 -0.20 -0.10 -0.08 -0.06 Comparative Formulation Example 1 0.05 0.04 0.02 -0.01 0.00 Comparative Formulation Example 2 -0.20 -0.15 -0.10 -0.05 -0.04 Comparative Formulation Example 3 -0.21 -0.15 -0.06 -0.05 -0.03 Comparative Formulation Example 4 -0.18 -0.16 -0.05 -0.03 -0.03

R1: Difference between the maximum value and the minimum value of the wrinkle contour line

R2: The wrinkle contour is arbitrarily divided into 5 squares, and the average value of R1 values

R3: Maximum value of R1 divided by 5

R4: Mean value of the baseline of the wrinkle contour minus the value of each apex and valley

R5: Mean value of the value obtained by subtracting the outline of each wrinkle from the base line of the wrinkle contour line

The results of measurement of the roughness and wrinkles of the skin applied with Formulation Example 1 using PRISMO are shown in FIG. The results after 8 weeks of using the product showed that the wrinkles became thinner and the skin roughness was improved much better than before.

As can be seen from Table 3 and FIG. 3, the compositions containing the palmytoyltripeptide-5 or diaminopropionyl tripeptide-33 of the present invention or polymerases containing the hydrolase moiety (Formulation Examples 1 to 4 ) Was superior to the comparative formulations 1 to 4 in improving the wrinkles of the skin. Particularly, in the case of Formulation 3 containing a polymerase comprising diaminopropionyl tripeptide-33 and hydrolyzed caprylic acid, the wrinkle-improving effect was superior to that of each of the single polymeric compounds. It was confirmed that the wrinkles of the skin can be improved more effectively by increasing the penetration into the skin by collecting the active ingredients in the polymer moss.

Thus, it was found that the cosmetic composition according to the present invention effectively improves and alleviates the wrinkles of the skin and has an excellent anti-aging effect.

[Test Example 3] Carbonization protein improvement evaluation

The applicants with severe dull skin were divided into groups of 20 persons to receive the cosmetic compositions of Formulation Examples 1 to 4 and Comparative Formulation Examples 1 to 4 and to use the product at least once a day for four weeks. The results of measuring the amount of carbonized protein in the same area of the facial skin before use and after 2 weeks and 4 weeks of use are shown in Fig. To analyze the amount of carbonized protein, several layers of keratin were sampled and stained with a fluorescent material. The portion with carbonized protein appeared green and the keratin was observed with a microscope at a magnification of 100 times. The results are shown in Fig.

Immunofluorescent staining slides were coated with keratin and the material was treated for 48 hours before immunofluorescence staining. Detailed immunofluorescence staining method is as follows. The keratinous skin was washed twice with D-PBS (Dulbecco's phosphate buffered saline) and fixed with 3.5% paraformaldehyde for 10 minutes. Fixed keratinous skin was washed three times for 10 minutes with D-PBS, and treated with 0.1% Triton X-100 (Triton X-100) for 5 minutes to penetrate the keratin skin. Washed with phosphate buffered saline (PBS) for 10 minutes and blocked with 5% goat serum for 30 minutes. The blocked horny layer was treated with 5% goat serum supplemented with primary antibody and allowed to react at room temperature for 1 hour to allow primary antibody to bind to the anti-antibody. After washing with D-PBS three times for 10 minutes, the excess primary antibody was removed and the secondary antibody was treated at room temperature for 30 minutes. And washed with PBS three times for 10 minutes to completely remove the secondary antibody. A drop of mounting solution was dropped onto the slide glass and covered with a cover slip. The excess mounting solution from the cover slip was removed and the cover slip was sealed. Then, the difference in fluorescence of each experimental group was observed, and the amount of reduced protein was confirmed. The results are shown in FIGS. 4 and 5.

In the results of FIG. 4, in particular, Formulation Example 3, which contains a polymerase containing diaminopropionyl tripeptide-33 and a hydrolized sugar chain, removes the carbohydrate protein in the stratum corneum of the skin, And about 45%, respectively. In addition, compositions using raw materials collected by polymerases (Formulation Example 1 (comparative formulations 1 to 3) and Comparative Examples 2 to 4) containing a diaminopropionyl tripeptide-33 or hydrolyzed precursor ~ 4), the effect was maximized.

In addition, in the results of FIG. 5, it can be confirmed that in Formulation Example 2 containing the polymerase in which diaminopropionyl tripeptide-33 was captured, the number of carbonized proteins stained with green fluorescence decreased, It was confirmed that the decrease of the carbonized protein caused by the skin was effective in improving the skin transparency.

[Test Example 4] Promotion of collagen synthesis promotion

Human fibroblasts were cultured in a 24-well plate incubator and then replaced with medium containing the test substance at the concentrations shown in Table 4. [ On the third day of culture, DMEM medium containing 10% fetal bovine serum was added in an amount of 0.5 ml per well, and then 10 μCi of L [2,3,4,5- ³ H] -proline was added. After 24 hours, the media and cells in each well were scraped off, washed with 5% trichloroacetic acid (TCA) solution, and then divided into two test tubes. One test tube contained type I collagenase type I collagenase) was added at 37 ° C for 90 minutes and the other tubes were stored at 4 ° C. Then, 0.05 ml of 50% trichloroacetic acid was added to all test tubes, and the mixture was allowed to stand at 4 ° C for 20 minutes, and then centrifuged at 12,000 rpm for 10 minutes at each time. Each of the supernatant and the precipitate was diluted with a liquid scintillation counter (LSC) Counter (CPM) values were obtained, and RCB (Relative Collagen Biosynthesis) was measured for the control group and the experimental group according to the following equation (1), and the results are shown in Table 4 below .

Test substance Collagen biosynthesis value (RCB) (%) Control group 100 Palmitoyl tripeptide-5 (10 ppm) 129 Diaminopropionyl tripeptide-33 (10 ppm) 118 Hydrolyzed millet (10 ppm) 109 Diaminopropionyl tripeptide-33 (10 ppm) + hydrolyzed precursor (10 ppm) 135

From the results of Table 4, it can be seen that the palmitoyltripeptide-5 or diaminopropionyl tripeptide-33 according to the present invention increases collagen biosynthesis of fibroblasts.

 [Test Example 5] Skin elasticity improvement effect

In order to test the effect of improving the elasticity of the human skin, the following tests were conducted using Formulation Examples 1 to 4 and Comparative Formulation Examples 1 to 4 as follows. The skin elasticity was measured by using a skin elasticity tester (Cutometer SEM 575, C + K Electronic Co., Germany) after applying to the face for 4 weeks twice a day, morning and evening. And the results are shown in Table 5 below. The results in Table 5 indicate the viscoelasticity of the skin elasticity measuring device.

Test substance Skin elasticity effect Formulation Example 1 0.42 ± 0.12 Formulation Example 2 0.29 ± 0.09 Formulation Example 3 0.48 0.10 Formulation Example 4 0.31 ± 0.12 Comparative Formulation Example 1 0.20 ± 0.09 Comparative Formulation Example 2 0.32 ± 0.12 Comparative Formulation Example 3 0.22 0.09 Comparative Formulation Example 4 0.21 + - 0.12

From the results of Table 5, it can be confirmed that Compounds 1 to 4 containing the peptide polymer according to the present invention have better skin elasticity than Comparative Formulation Example 1.

Comparing Formulation Examples 2 to 4, in which the polymerase technology was not used, and compositions (Formulation Examples 1 to 4) in which the peptide was stabilized with the polymerase, I was able to see more excellence.

[Test Example 6] Evaluation of skin stability

In order to confirm the skin safety of the composition of the present invention, a patch test in which Formulation Examples 1 to 4 and Comparative Formulation Examples 1 to 4 were applied to 18 adult males and 12 males (average 32.5 years) was carried out, Were evaluated for skin stability.

Twenty - eight hours after the application of the patch, the patch was removed, and the first reading was performed 30 minutes later and the second reading after 96 hours. To determine the intensity of the skin irritation of the sample, weights were assigned according to the degree of the positive reaction of the skin, and the average skin irritation degree of the skin was visually determined. The results are shown in Table 6 below.

Test substance Average reactivity Judgment grade Formulation Example 1 0.000 No stimulation Formulation Example 2 0.000 No stimulation Formulation Example 3 0.000 No stimulation Formulation Example 4 0.000 No stimulation Comparative Formulation Example 1 0.000 No stimulation Comparative Formulation Example 2 0.000 No stimulation Comparative Formulation Example 3 0.000 No stimulation Comparative Formulation Example 4 0.000 No stimulation

From the results of Table 6, it was confirmed that Formulation Examples 1 to 4 and Comparative Formulation Examples 1 to 4 did not stimulate the skin.

Therefore, the cosmetic composition according to the present invention can be judged as having excellent skin stability.

1 is a schematic view showing a process of synthesizing a polymeric compound according to the present invention.

Fig. 2 shows the results of measurement of the water content of the facial skin at the time point 2 weeks and 4 weeks after application of the cosmetic composition of the present invention and after application.

Fig. 3 shows the results of measurement of the skin roughness and wrinkles of the skin of the eyes at eight weeks after application of the cosmetic composition of Formulation Example 1 and after application.

Fig. 4 shows the results of measurement of the amount of carbonized protein in the facial skin at 2 weeks and 4 weeks after application of the cosmetic composition of the present invention and after application.

Fig. 5 shows the result of staining a carbonized protein of a stratum corneum of a subject using Formulation Example 2. Fig.

Claims (8)

A cosmetic composition for moisturizing the skin, which comprises as an active ingredient a polymer obtained by capturing diaminopropionoyl tripeptide-33. delete The cosmetic composition according to claim 1, wherein the polymer is produced by synthesizing polylactic acid and polyethylene glycol at a weight ratio of 5: 2. The cosmetic composition according to claim 1, wherein the composition further comprises a polymerized product having a hydrolyzed capillary. A cosmetic composition for improving skin transparency, which comprises as an active ingredient a polymer obtained by capturing diaminopropionoyl tripeptide-33. delete 6. The cosmetic composition according to claim 5, wherein the polymer is produced by synthesizing polylactic acid and polyethylene glycol at a weight ratio of 5: 2. 6. The cosmetic composition according to claim 5, wherein the composition further comprises a polymerase having a hydrolyzed capped moiety.

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