JP2001278769A - Skin cosmetic - Google Patents

Abstract

PROBLEM TO BE SOLVED: To obtain a skin cosmetic which has a synergistically promoted skin- tightening effect and enhanced safety, improves the wrinkles and sagging of skins, and gives tensive and beautiful skins. SOLUTION: This skin cosmetic contains an integrin production-stimulating agent (one or more algae or plants selected from Fucus vesiclosus or Fucus evanescens, Laminaria japonika Areschoug, Hizikia fusiforme Okamura, Rosmarinus officinatis, Actinidia chinensis, Panax ginseng C. A. Meyer, Poria cocos Wolf, Prunus persica Batsch, and Arctium lappa L., or their extracts) and an actin-myosin-based activating agent (one or more plants selected from Scutellaria baicalensis Georgi, Phellodendron amurense Ruprecht, Gardenia jasminoides Ellis, Rehmannia glutinosa Liboschitz var. purpurea Makino, Zingiber officinale Roscoe and Astragalus sinicus, and their extracts).

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a skin cosmetic which improves skin elasticity and gives skin firmness.

[0002]

2. Description of the Related Art The sagging of the skin due to aging of the skin causes a decrease in the elasticity of the dermis connective tissue of the skin, a decrease in the supporting force of the subcutaneous adipose tissue, and a decrease in the muscle strength supporting the skin. It is thought to be caused by lowering etc.
In order to improve this, cosmetics containing skin tightening agents such as hibamata, caffeine, and saikosaponin have been known (JP-A-5-124953, JP-A-10-72336, and JP-A-10-72336). JP-A-5-17332, etc.).

[0003] However, when such a skin tightening agent is used alone, the effect is not always sufficient.
In order to actually act on the inside of the skin to tighten the skin and effectively improve the sagging, it was necessary to mix a tightening agent at a high concentration and use it for a long period of time.

[0004] It is an object of the present invention to effectively improve sagging of the skin and provide a safer skin cosmetic.

[0005]

Means for Solving the Problems The inventors of the present invention have examined the mechanism of generation of skin wrinkles and sagging and components that improve the elasticity of the skin, and found that the specific plant component activates the actin-myosin system. It has been found that, when these actin-myosin activators and integrin production promoters are used in combination, the sagging of the skin is effectively improved and firm and beautiful skin can be realized.

That is, the present invention provides a skin cosmetic containing an integrin production promoter and an actin-myosin activator.

[0007]

BEST MODE FOR CARRYING OUT THE INVENTION The cosmetic of the present invention contains an integrin production promoter and an actin-myosin activator. Here, integrin is expressed on the cell surface and is involved in cell adhesion. The structure is composed of α subunit and β subunit. It is known that α subunit has α1 to α5, αL, etc., and β subunit has β1, β2, β3, etc. Have been. The present inventors have found that a specific natural component has an action of promoting the production of integrin, which is useful as a cancer metastasis inhibitor (JP-A-11-246428), and which has an effect of improving sagging of skin and tightening of skin. It has been found that this is the case (Japanese Patent Laid-Open No. Hei 10-72336).

On the other hand, in recent years, tension of the skin (actin-myosin bundle) present in fibroblasts in the skin is involved in skin firmness, and activation of the actin-myosin system via G protein (actin-myosin bundle) is performed. The mechanism of actin-myosin system activation has been elucidated, suggesting that a substance that activates the actin-myosin system is useful for improving skin wrinkles and sagging. However, it has not been known at all heretofore that the elasticity of the skin is significantly improved by using a component that promotes integrin production in combination with a component that activates the actin-myosin system.

[0009] The integrin production promoter of the present invention promotes the production of integrin,
Collagen, vitronectin,
Considering the interaction between matrices such as fibronectin and laminin and cells present in connective tissues such as fibroblasts, those that promote the expression of α2 subunit, α5 subunit and β1 subunit are preferable, and α2 subunit is more preferable. What promotes the expression of β1 subunit at the same time is more desirable. There are almost no such integrin production promoters. For example, transforming factor (TGFβ) is known as a single compound, and seaweeds such as hibamata, makombu and hijiki, rosemary, kiwi, carrot, bukuro, tonin and burdock From the viewpoint of promotion of integrin production and safety, seaweeds selected from Hibamata, Macomb and Hijiki, plants selected from rosemary, kiwi, carrot, bukuro, Tonin and burdock, or extracts thereof. Is particularly preferred.

In the present invention, “Hibamata” refers to Fucus vesiclosus of the genus Hucamata (Fucaceae).
Or Fucus evanescens, and the kelp is the family Laminariaceae (Lami
nariaceae) L.japonika Aresc of the genus Laminaria
houg, Hijiki means H. fusiforme Okamura of Sargassaceae, Hizikia, and Rosemary Rosmarinus officinatis of Labiatae.
The kiwi is a kiwi (Act) of the genus Actinidia
inidia chinensis, carrot and
iaceae) Panax ginseng CA Meye
r (Panax schinsengNees)) excluding the fine roots or those obtained by lightly blanching the roots. Bukryou is a pine beet (Poria cocos Wolf) of the family Polyporaceae.
The sclerotium excluding most of the outer layer of the peach is called tounin, peach (Prunus persicaBatsch or Prun
The seeds of us persica Batsch var. davidiana Maximowicz) and burdock are Arctium lapp of Compositae
a Indicates L.

On the other hand, the actin-myosin activator of the present invention refers to a substance which acts on skin fibroblasts to promote their cell tension, and has been used so far with whey protein made from milk and hydrolyzate. It has been reported that degraded casein has this effect. The present inventors, as shown in Example 2 described below, each of the plant extracts of Ogon, Obak, Gardenia, Jio, Ginger, and Astragalus have an effect of promoting the tension of dermal fibroblasts, −
It was found to be a myosin-based activator.

[0012] Here, "Ogon" refers to Labiatae
Scutellaria baicalensis Georgi (Scutellaria baicalensis Georgi) refers to the roots excluding the pericarp, oubak refers to the tree of Rutaceae (Rutaceae) without the pericarp, or to the gardenia (Rutaceae). Rubiaceae) Gardenia jasminoides Ellis, and Jio is a red croaker (Rehmannia glutinosa Liboschitz var. Purpur) of Scrophulariaceae.
ea Makino or Rehmannia glutinosa Liboschitz) or steamed roots, ginger is a ginger (Zingiber officina) of the Zingiberaceae family
le Roscoe) rhizome, and astragalus are legumes (Legumin)
osae) of Astragalus sinicus.

In the algae of the present invention, one or more of the whole algae, roots, stems and leaves can be used as they are or pulverized. For plants, the whole plant or leaves, roots, fruits and seeds of the plant can be used. , One or more of the flowers can be used as they are or crushed.

The extract in the present invention means various solvent extracts obtained by extracting the extract at room temperature or under heating or by using an extraction device such as a Soxhlet extractor, Liquid, its concentrated liquid or its dried powder. Here, the extract may be derived from one plant or from two or more plants.

As the extraction solvent used for obtaining the algae and plant extracts of the present invention, any of a polar solvent and a non-polar solvent can be used. For example, water; alcohols such as methanol, ethanol, propanol and butanol; polyhydric alcohols such as propylene glycol and butylene glycol; ketones such as acetone and methyl ethyl ketone; esters such as methyl acetate and ethyl acetate; tetrahydrofuran and diethyl ether Chain and cyclic ethers such as polyethylene glycol; polyethers such as polyethylene glycol; halogenated hydrocarbons such as dichloromethane, chloroform and carbon tetrachloride; hydrocarbons such as hexane, cyclohexane and petroleum ether; fragrances such as benzene and toluene. Group hydrocarbons; pyridines and the like, and these can be used as a mixture of two or more kinds.

The above extract can be used as it is, but it can also be used after diluting, concentrating or freeze-drying the extract and then preparing it as a powder or paste.

The extract may be used after removing inactive contaminants from the extract by a technique such as liquid-liquid distribution, and such an extract is preferably used in the present invention. These may be used after being subjected to a treatment such as deodorization and decoloration by a known method as necessary.

The composition containing such an integrin production promoter and actin-myosin activator has an extremely excellent collagen gel shrinking action and an excellent skin effect as shown in Examples 3 and 4 described below. It shows an elasticity improving effect and a tightening effect. Accordingly, the composition is effective for external stimuli such as drying and ultraviolet rays, and various changes in skin properties caused by aging of the skin, for example, skin wrinkles, sagging, abrasion and loss of elasticity. It can be used as a cosmetic, or as an elasticity improver, a sag improver, or the like.

In the skin cosmetic of the present invention, the integrin production promoter is preferably incorporated in the composition in an amount of 0.001 to 10% by weight, and when the above algal or plant extract is used as the integrin production promoter. Is 0.00001 to 1% by weight in terms of solid content, particularly 0.0001 to 0.1
It is preferable that the content be contained by weight.

The actin-myosin activator comprises
The composition preferably contains 0.001 to 10% by weight, and when the above-mentioned plant extract is used as an actin-myosin activator, 0.00001 to
It is preferably contained in an amount of 1% by weight, particularly 0.0001 to 0.1% by weight.

In addition to the integrin production promoter and actin-myosin activator, the skin cosmetic composition of the present invention may contain a commonly used external base material and other active ingredients. The external base material used herein may be any of those based on an oily base, those based on an oil / water or water / oil type emulsified base, and those based on water. .

The oleaginous base includes, for example, vegetable oils, animal oils, synthetic oils, silicone oils, fatty acids, natural and synthetic glycerides, and the like. Further, humectants, ultraviolet absorbers, alcohols, chelates, pH adjusters, preservatives, thickeners, pigments, fragrances, and the like can be arbitrarily combined and compounded. The medicinal component is not particularly limited,
For example, an analgesic antiphlogistic, a germicidal antiseptic, vitamins, emollients, etc. can be used as needed. Examples of the form of the skin cosmetic include ointments, creams, emulsions, lotions, gels, packs, cataplasms, foundations, and the like.

The amount of the skin cosmetic of the present invention is 1 day, 1 day
100 to 500 mg, preferably 200 mg per serving is preferred.

[0024]

EXAMPLES Production Examples 1 to 22 1 kg of pulverized material of each part of seaweeds and plants shown in Table 1 was immersed in 5 liters of an extraction solvent at room temperature for one week to extract solvent-soluble components. The same operation was repeated for the residue from which the extract was separated to obtain a total of 10 liters of the extract. The solvent of this extract was distilled off and dried under reduced pressure to obtain an extract. In addition,
In the following, W represents water, BG represents 1,3-butylene glycol, and ET represents ethanol.

[0025]

[Table 1]

Example 1 Evaluation of Promotion of Integrin Production The amount of fibroblast surface integrin was measured by FACScan. That is, various samples shown in Table 1 were allowed to act on fibroblasts in monolayer culture at 30 to 100 μM, and after 24 hours, the cells were detached with trypsin / EDTA, neutralized with FCS, and then cultured with 0.01% FCS. , 0.02% N
The cells were washed with PBS containing aN ₃ and collected. Anti-human integrin α2β1, anti-human integrin α2, and anti-human integrin β1 were diluted 100-fold as the primary antibody, and FITC-labeled anti-mouse IgG1 was diluted 100 times as the secondary antibody.
Used in double dilution. Blank is mouse IgG1 as primary antibody
Was used. Table 2 shows the results.

[0027]

[Table 2]

Example 2 Evaluation of Cell Tension Generation by Activation of Actin-Myosin System Kolodney et al. (Kolodeney MS et al., J. Cell Biol., 1
17, 73-82 (1992)), a fibroblast-embedded collagen gel was prepared, and the obtained gel was fixed in a serum-free medium kept at 37 ° C., and a load of 50 to 100 mg was applied and stabilized. After that, 1 mL of a sample shown in Table 3 adjusted to about 50 times the final concentration with serum-free DMEM was added (concentration 0.001%, evaporation residue weight%), and the generated force was determined by Isoto.
Measured by nic Transducer and recorded by BIOPAC. The resulting tension was defined as the average difference between the relatively stable 5 minutes before addition of the sample and the 5 minutes after which the tension after addition was maximum. The results are shown in Table 3.

[0029]

[Table 3]

Example 3 Influence on Collagen of Fibroblast-Embedded Collagen Gel Collagen gel is described in J. Cell Science, 102, 315 (19).
92) "or" J. Invest. Dermatol, 93, 792 (1989) ". That is, a collagen gel solution (tapel-A (3.0 mg / ml, pH = 3) manufactured by Nitta Gelatin Co., Ltd.) under ice cooling.
HEPES (250 mM) in 0.05 N sodium hydroxide solution, DMEM (GIBCO DMEM, low glucose) 5-fold concentrated solution,
FCS (2%, Fatal Calf Serum) and purified water were added, and the mixture was thoroughly stirred and neutralized, and then the test substances having a final concentration of 0.01 to 0.001% by weight (evaporation residue weight%) shown in Tables 4 and 5 (The seaweed or plant extract obtained in Production Examples 1 to 23 and ethanol or 1,3-butylene glycol as a control) were added, and finally, a suspension of human skin fibroblasts (derived from human foreskin) was added. After sufficiently stirring to remove air bubbles, 1 ml of each well was poured into a 24-well plate, and immediately gelled at 37 ° C. The collagen concentration at this time was 1.
It was adjusted to 5 mg / mL. After 24 hours, peel around the gel,
The medium was added and cultured. Gel volume measurement is described in the literature (J. Cell
Science, 102, 315 (1992)). That is, 10% formalin fixation (4 ° C, 24 hours)
Then, the surface tension of the water was adjusted to Triton XlOO (manufactured by Wako Pure Chemical Industries, Ltd.) (1
%) And then weighed. The results are shown in Tables 4 and 5.

[0031]

[Table 4]

[0032]

[Table 5]

It was shown that the combined use of an integrin production promoter and an actin-myosin activator reduced the diameter and volume of a fibroblast-embedded collagen gel and synergistically promoted gel shrinkage.

Example 4 Using the samples shown in Table 6, the degree of improvement in skin sagging (cheek) was evaluated by panelists. Each sample was evaluated by a panel of 10 panelists using a gel of HIBAMATA extract alone or a mixture of HIBAMATA extract and Ginger or Oba. Each extract-containing gel was applied once a day, in the morning and at night, to the area from the cheek to the chin for 2 weeks. The following scores were used for evaluation. The results are shown in Table 6.

-1: Deteriorated 0: No change, I do not know 1: Slightly improved slack 2: Slightly improved slack 3: Improved slack 4: Very improved slack

[0036]

[Table 6]

From Table 6, the tightening effect was felt for the samples C and D of the present invention.

Example 5 Formulation Examples Various components of the present invention were prepared by stirring and mixing the components shown in Tables 7 to 12 in a conventional manner.

[0039]

[Table 7]

[0040]

[Table 8]

[0041]

[Table 9]

[0042]

[Table 10]

[0043]

[Table 11]

[0044]

[Table 12]

[0045]

EFFECT OF THE INVENTION Integrin production promoter and actin
The skin cosmetic of the present invention containing a myosin-based activator,
Compared with the case where the integrin production promoter or actin-myosin activator is used alone, the skin tightening effect is synergistically promoted, and since it is safer, wrinkles and sagging of the skin are improved. It is useful as a cosmetic that prepares firm and beautiful skin.

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Claims (4)

[Claims] 1. An integrin production promoter and actin-
Skin cosmetics containing a myosin-based activator. 2. The integrin production promoter is Hibamata,
Macomb, Hijiki, Rosemary, Kiwi, Carrot,
2. The skin cosmetic according to claim 1, which is at least one kind of seaweed or plant selected from bukuro, tonin and burdock, or an extract thereof. 3. The skin cosmetic composition according to claim 1, wherein the actin-myosin activator is at least one plant selected from Japanese gourd, oak, gardenia, zio, ginger, and astragalus or an extract thereof. Fees. 4. The skin cosmetic according to claim 1, wherein the skin cosmetic improves skin elasticity.