KR101470707B1 - A cosmetic composition comprising extracts of Rhynchosia nulubilis peel for whitening skin - Google Patents

Abstract

The present invention relates to a cosmetic composition for skin whitening containing an extract of Bombyx mori. The extract according to the present invention inhibits the production of melanin, excellently skin whitening effect, and can be used as natural cosmetics.

Description

[0001] The present invention relates to a cosmetic composition comprising extracts of Rhynchosia nulubilis peel for whitening skin,

The present invention relates to a skin whitening cosmetic composition containing an extract of Seomyeonpo.

In recent years, a healthy and relaxed life has been demanded, and with the desire to be beautiful, transparent and clear skin has become a dream for everyone. As evidenced by this, the preference for skin whitening cosmetics has expanded from summer to all seasons, leading to the development of more whitening lines.

The skin color of a person is determined by the amount of melanin, carotene and hemoglobin, and melanin is the most crucial factor. Melanin is synthesized in the melanocyte, which is a pigmentary cell in the epidermal basal layer, and transformed into keratinocyte, which shows skin color. It disappears with keratinization along with the keratinization of the epidermis. In addition, melanin prevents ultraviolet rays from absorbing or scattering ultraviolet rays from the skin to prevent cells or tissues inside the skin from being damaged by ultraviolet rays. However, melanin is abnormally excessive due to external environmental changes such as exposure to ultraviolet rays, If the melanin is not completely eliminated due to lack of production or keratinization, pigmentation may occur, which may cause blackening of the skin, spots, and freckles. Such skin disorders can be a cosmetic problem for the patient and can cause serious psychological problems. In addition, unsightly facial lesions can have a profound effect on emotions and health and cause a reduction in social functioning and productivity in work and school.

Melanin synthesis in melanocytes is described in detail. An enzyme called tyrosinase acts on tyrosine as a substrate to generate dopaquinone and spontaneous reaction from dopaquinone And enzyme reaction to produce melanin in the copolymer black pigment. Therefore, in order to prevent pigmentation of the skin, it is general and easy to reduce the production of melanin by inhibiting some reactions during the melanin production process.

Since the 1980s, skin cancer researchers have studied the biosynthetic pathway of melanin, which has led to whitening cosmetics and drug development. In particular, kojic acid, arbutin, ascorbic acid, etc. have been developed and utilized in the development of a tyrosinase inhibitor, which is known as a melanin biosynthesis rate-limiting enzyme. However, COZSAN has been discontinued due to the problem of carcinogenicity to thyroid cancer, and arbutin has been reported to have weak activity. In addition, ascorbic acid is easily oxidized in a solution to be browned and lose its whitening activity. Therefore, it is troublesome to use ascorbic acid in its derivation without being able to use it alone.

In addition, many studies have been carried out to find whitening active ingredients in natural products, especially plants. Among them, many plant extracts and herbal medicine extracts such as alfalfa, licorice, peony, cinnamon, gossam, It has been found that they have an inhibitory activity against azithromycin. However, these also have many problems in using cosmetics or medicines at an effective concentration or more in terms of safety and possibility of discoloration, and there is a limit in that whitening effect is not clear.

Therefore, there has been continued research on natural raw materials which are excellent in stability in cosmetic compositions and excellent in whitening effect without stimulation to the skin, in particular, natural raw materials which are easily available in the domestic market.

Korean Patent Laid-Open Publication No. 10-2005-0104934 (published on November 03, 2005)

It is an object of the present invention to provide a cosmetic composition for skin whitening which is excellent in skin whitening effect by inhibiting the production of melanin.

The present inventor has sought to find a skin whitening substance. As a result, the present inventors have completed the present invention by confirming that the extract of Seomotari peel based on the two methods has a skin whitening effect by inhibiting melanin production.

The present invention relates to a process for the preparation of Rhynchosia nulubilis skin extract of the present invention.

According to the present invention, it is preferable that the saxiflorium bark is dried with a shoot of 5 to 10 mm, then peeled or dried, or peeled and dried without sprouting.

The extract according to the present invention may be used in combination with water, C1-C4 alcohol, ethyl acetate, glycerin, ethylene glycol, propylene glycol, butylene glycol and mixed solvents thereof (e.g., A propylene glycol, a hydrolyzed propylene glycol, a hydrolyzed propylene glycol, a hydrolyzed glycol, and a propylene glycol).

According to the present invention, the extract preferably contains 0.001 to 5% by weight based on the total weight of the composition.

The cosmetic material according to the present invention is selected from the group consisting of solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, surfactant-containing cleansing, oils, powder foundations, emulsion foundations, wax foundations and sprays Lt; / RTI >

The cosmetic composition containing the extract of Bombyx mori L. according to the present invention inhibits melanin production and is excellent in skin whitening effect.

Figure 1 shows the inhibitory effect of Bombyx mori extract on the production of melanin in B16F10 cells.
Fig. 2 shows the effect of inhibiting the melanin formation in the artificial tissue by the extract of Bombyx mori.

Hereinafter, the present invention will be described in detail.

Seomok-tae  Bark extract Cosmetics  Composition

The present invention provides a cosmetic composition containing an extract of Bombyx mori.

Rhynchosia nulubilis is also known as the bean, which is a perennial plant of soybean and has brown stem and leaf. A yellow flower blooms in July, and a black and round fruit about 5 to 7 mm in diameter is harvested in the oval-shaped cutter. According to the mainchain gangbok, it is better to use a small soybean powder as a medicine while the light is black. Sulfur spraying and growing are known to improve the mildness, warmth, flavor and toxicity.

Foje is a pharmaceutical technology that changes the original properties of medicines by processing the medicines based on the oriental medicine theory. Some medicines are toxic or very strong in nature and can not be taken directly. Some medicines can not easily be stored for a long time and others can be used only after removing some parts and parts. Also, even if the same medicines are used, there is a case where the properties of the raw material and the raw material are not same or different from each other. Before using these medicines, they must be processed, which is called the form of medicinal plants.

Foams are sometimes processed together by adding a supplement, and the use of the supplement is to achieve the purpose of the drug according to the dialect. Because of the variety of types of pills used in drug formulations, they have different properties and actions, and the resulting action of the medicinal formulations is different. There are a myriad of commonly used types of supplements, but generally they can be broadly categorized as liquid supplements and solid supplements. Liquid ingredients include alcohol, vinegar, honey, ginger juice, licorice juice, black bean juice, saline, unmeaned water, milk, urine (urine of children under the age of 7) and lime water. Solid foods include rice, bran, Tofu, earth, ply, sand, etc. are used.

The purpose of purifying herbal medicine is to ① clean the drug and make it easy to store ② reduce or eliminate the toxicity and side effects of the drug ③ change the drug to make the drug more effective ④ improve the therapeutic effect of the drug And (5) to remove the bad smell and taste of the medicinal materials so as to make them good for taking.

There are various methods in the formulations, but in the present invention, it is preferable to formulate the formulations according to the two methods.

The two methods are the seeding method which takes the seeds of the bean, usually shoots the seeds to make the seeds bloom, then strips the seeds and crushes them.

It was confirmed that the extract of Bombyx mori L. exhibited by the two methods according to the present invention inhibited melanin production and showed excellent whitening effect. Accordingly, the extract of Seomari Seaweed, which has been foamed by the two methods according to the present invention, can be included in a cosmetic composition for improving skin wrinkles.

The content of the extract of Bombyx mori L. is 0.000001 to 30.0% by weight, more preferably 0.0001 to 15.0% by weight, and most preferably 0.001 to 5.0% by weight based on the whole cosmetic composition.

The ingredients contained in the cosmetic composition of the present invention include components commonly used in cosmetic compositions in addition to the extract of Seomari Seabull which is formulated by the two methods as an active ingredient, and examples thereof include antioxidants, stabilizers, solubilizers, vitamins, Customary adjuvants such as perfumes, and carriers.

The cosmetic composition of the present invention can be prepared into any of the formulations conventionally produced in the art and can be used as a solution, a suspension, an emulsion, a paste, a gel, a cream, a lotion, a powder, a soap, , Oil, powder foundation, emulsion foundation, wax foundation and spray, but is not limited thereto. More specifically, it can be manufactured in the form of a soft lotion, a nutritional lotion, a nutritional cream, a massage cream, an essence, an eye cream, a cleansing cream, a cleansing foam, a cleansing water, a pack, a spray or a powder.

When the formulation of the present invention is a paste, cream or gel, an animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as the carrier component .

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. In the case of a spray, in particular, / Propane or dimethyl ether.

When the formulation of the present invention is a solution or an emulsion, a solvent, a dissolving agent or an emulsifying agent is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, , 3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan fatty acid esters.

In the case where the formulation of the present invention is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Cellulose, aluminum metahydroxide, bentonite, agar or tracant, etc. may be used.

When the formulation of the present invention is an interfacial active agent-containing cleansing, the carrier component may include aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives, or ethoxylated glycerol fatty acid esters.

Method of producing extract

The extract of Seomari Seabull which has been foamed by the two methods according to the present invention can be manufactured as follows, but is not limited thereto.

(1) The step of processing the western style in

Seomok-tae is immersed in water for 5 to 10 hours to remove wrinkles on the skin. Place the tray in a container with a drain and cover it with a damp cloth. Spray water 1 to 5 times daily, maintain temperature 18-25 ℃, and humidity 40-45%. After 10 to 14 days, if the shoots of Seomuktae are 5 ~ 10 mm long, remove them and rub them, peel off the seeds and crush them. However, the present invention is not limited to this, and the seed coat can be peeled off without sprouting the seedling.

(2) Extraction step of the extract

The extract is extracted by adding the extracting solvent to the fungicide-treated bark of Seomjak, and the extract is cooled, filtered, concentrated under reduced pressure and dried to obtain the Fungicosa extract.

The extraction may be by shaking extraction, Soxhlet extraction, or a reflux extraction method, but is not limited thereto. The extraction temperature is preferably 40 to 100 캜, more preferably 60 to 80 캜. The extraction time is preferably 2 to 24 hours, and the extraction time is preferably 1 to 5 times.

Examples of the extraction solvent include water, C1-C4 alcohol, ethyl acetate, glycerin, ethylene glycol, propylene glycol, butylene glycol and mixed solvents thereof (for example, a functional alcohol, a functional glycerin, a functional ethylene glycol, Functional butylene glycol), and it is preferable to use 1,3-butylene glycol. In addition, it is preferable to add 5 to 15 times the weight or volume of the extraction solvent to the seedling shell, which has been foamed by the above two methods, more preferably 10 times.

It is preferable to use a vacuum decompression concentrator or a vacuum rotary evaporator for the above-mentioned reduced pressure concentration, and it is preferable to use a vacuum drying method, a vacuum drying method, a boiling method, a spray drying method or a freeze drying method. However, the present invention is not limited thereto.

Hereinafter, the present invention will be described in more detail with reference to the following examples and experimental examples. However, the following examples should not be construed as limiting the scope of the present invention, but should be construed to facilitate understanding of the present invention.

< Manufacturing example  1> By the law of two Foo  Treated Seomok-tae  Preparation of extract

(1) The two methods

I bought 500g of Seomok-tae, which was purchased from the medicine allowance, in water for 6-8 hours to remove the wrinkles on the skin. The pellets were placed in a container with a drain, covered with a damp cloth, water was sprinkled twice a day, and the temperature was maintained at 18-25 ° C and the humidity was 42-45%. After 10 to 14 days, if the buds of Seomoktae were 6 ~ 9 mm long, they were taken out, rubbed and rubbed, and the seed coat was peeled off and crushed.

(2) Extraction

After the addition of 50% 1,3-butylene glycol (1,3-BG), 10 times the amount of 1,3-butylene glycol (1,3-BG) was added and the mixture was refluxed at 80 ℃ for 3 hours. The filtered extract was concentrated on an evaporator at 80 ° C and 80 rpm until all the water evaporated.

< Manufacturing example  2> By the law of two Foo  Untreated Seomok-tae  Preparation of extract

In the two methods, 50% 1,3-butylene glycol (1,3-BG) was added to 10 times as much untreated western blotter and the mixture was refluxed at 80 ° C for 3 hours in a water bath. The filtered extract was concentrated on an evaporator at 80 ° C and 80 rpm until all the water evaporated.

< Example  1> B16F10 From melanoma  Measurement of inhibition of melanin formation

In order to confirm the whitening effect of Bombyx mori extract, the whitening effect of B16F10 melanoma was evaluated based on the degree of inhibition of melanin formation.

The B16F10 melanoma used in this Example is a mouse-derived cell line, and is a cancer cell caused by malignantization of a cell that secretes melanin, a black pigment. During the artificial incubation of these cells, the degree of decrease in melanin production was compared and evaluated. The B16F10 melanoma used in this experiment was purchased from Korean Cell Line Bank (KCLB No: 8008).

B16F10 Melanoma melanin biosynthesis inhibitory effect was measured as follows. B16F10 melanoma was subcultured in a 6-well plate at 5 × 10 ⁵ / well and cultured in a medium containing 10% FBS (fetal bovine serum) for about 24 hours. Samples were processed into fresh medium without FBS and aged for 72 hours. Trypsin-EDTA was added and the cells were separated by centrifugation. Melanin was dissolved at 95 ° C in 1N NaOH containing DMSO. The amount of melanin was measured by measuring the absorbance at 405 nm using an ELISA reader.

The control group was added with the extraction solvent, and the positive control group was the kojic acid added to the skin whitening composition.

The results are shown in Fig.

As shown in Fig. 1, it was confirmed that the amount of melanin was 28.7% lower than that of the control group when the Foaming-treated Seomari bark extract was added by the two methods. This is similar to the case of the addition of the positive control kojic acid (33.1% decrease).

< Example  2> Confirmation of Melanogenesis Inhibitory Effect on Artificial Tissue

The pad was placed on the tray and the culture medium was put into 4 ml per well to cultivate the artificial skin. After 24 hours, the medium was removed and the samples dissolved in the concentrations of 50, 500 and 5000 μg / ml were treated with 1 ml each. After 24 hours, the artificial skin was photographed using a dissecting microscope, and the medium was collected and stored at -80 ° C. 4 ml of the sample in which the sample was dissolved was treated. The above steps 2 to 4 were repeated daily for 5 days (1 day to 5 days of medium). On day 5, the media was collected and stored at -80 ° C, and the artificial skin was transferred to another tray and exposed to UVB (Ultra Violet B) at 60 mJ / cm ² . The artificial skin was transferred to the original tray and the sample was treated with 4 ml each. After 4 h, transferred to the artificial skin to the other tray and exposed to UVB 60 mJ / cm2 again leave transferred to the original tray, were once again repeat the process after 4 hours (i. E., Man-made to UVB of 60 mJ / cm ² The skin is exposed 3 times in 4 hours). After 48 hours, the medium was collected and stored at -80 ° C (7 days medium), and the artificial skin was separated from the insert and fixed in 10% formalin for 24 hours at 4 ° C. NO (Griess reagent assay) and GM-CSF assay were performed on each medium (1 to 5 days and 7 days). Each artificial skin was stained with hematoxylin-eosin and masson's trichrome by making a paraffin block.

The results are shown in Fig.

As shown in FIG. 2, it was confirmed that the amount of melanin was significantly reduced in the extract of Bombyx mori as determined by the two methods, and it was confirmed that the amount of melanin was significantly lower than that of arbutin used as a whitening composition.

Thus, the results of Examples 1 and 2 show that the extract of Seomari Seaweed, which has been foamed by both methods, has a skin whitening effect by reducing melanin production. Therefore, it is said that the germination extract of the present invention can be effectively used as an effective ingredient of a cosmetic composition for skin whitening.

Formulation Example  One. Seomok-tae  Manufacture of lotion using skin extract

1 kg of lotion (skin lotion) containing Seomari Seaweed extract powder (Preparation Example 1) was prepared by the following production method with the content of [Table 1] shown below.

[Table 1]

In Table 1, substances No. 2, 3, 4 and 8 were added to the 11th substance in order and dissolved in the reaction container, and the substance No. 5 was dissolved by heating to about 60 ° C. Subsequently, the substance No. 10 was dissolved in the reaction vessel and added to the substance No. 11. Finally, the materials 1, 6, 7, and 9 were added, stirred sufficiently, and aged at 25 ° C for 3 days to prepare the title lotion.

Formulation Example  2. Seomok-tae  Manufacture of nutrition lotion with skin extract

1 kg of hydrosome nutrition lotion containing Seomari Seaweed extract (Preparation Example 1) was prepared by the following production method in the content of [Table 2] below.

[Table 2]

In Table 2, materials 10, 11, 13, and 16 were heated to 80 to 85 ° C while mixing and stirring, and then charged into a manufacturing part. Then, an emulsifier was allowed to react and the mixture was stirred at 2, 3, 4, 5, 6, 7, 8, 9, and 12 were heated and dissolved at a temperature of 80 to 85 ° C, followed by emulsification. After the emulsification is completed, the mixture is cooled to 50 ° C with stirring using an agitator, and then the substance 15 is added. After cooling to 45 ° C, the substance 14 is added and the substance 1 is added at 35 ° C. For 3 days to prepare the nutritional lotion of the title.

Formulation Example  3. Seomok-tae  Nutrition with peel extract Serum  Produce

1 kg of hydrosomal nutrition serum containing Seomari Seaweed extract (Preparation Example 1) was prepared by the following production method in the content of [Table 3] below.

[Table 3]

In Table 3, materials 2, 3 and 5 were heated to between 40 and 45 ° C while mixing and stirring. Then, the mixture was put into a manufacturing part, and then an emulsifying agent was allowed to react and the materials 4, 6 and 7 were added to the manufacturing part and emulsified. After the emulsification was completed, the mixture was cooled to 35 ° C with stirring using an agitator, the material 1 was added thereto, cooled to 25 ° C, and aged at 25 ° C for 3 days to prepare the titled nutritional serum.

Formulation Example  4. Seomok-tae  Production of essence using bark extract

1 kg of hydrosomal essence containing Seomari Seaweed extract was prepared by the following production method with the content of [Table 4] below.

[Table 4]

In Table 4, materials 2, 3, 4, 5 and 6 were homogenized at a constant temperature to prepare nonionic amphipathic lipids. This nonionic amphipathic lipid was mixed with substances 1, 7, 8, and 14, homogenized at a constant temperature, passed through a microfluidizer, and then the substance No. 9 was heated to 50 to 60 ° C and gradually added to homogenize And again passed through the microfluidizer. Subsequently, substances 10, 11, 12 and 13 were added, dispersed and stabilized, and aged at 25 캜 for 3 days to prepare an essence of the title.

Claims (7)

Rhynchosia nulubilis ) skin extract. The cosmetic composition for skin whitening according to claim 1, wherein the bark of Seomari Seam is 5 to 10 mm of shoots, then peeled and dried, or peeled and dried without sprouting. The skin whitening cosmetic composition according to claim 1, wherein the extract is extracted with an extraction solvent selected from water, C1-C4 alcohol, ethyl acetate, glycerin, ethylene glycol, propylene glycol, butylene glycol and a mixed solvent thereof . The cosmetic composition for skin whitening according to claim 3, wherein the extraction solvent is 1,3-butylene glycol. The cosmetic composition for skin whitening according to claim 1, wherein the extract of Bombyx mori extract inhibits melanin production. The cosmetic composition for skin whitening according to claim 1, wherein the composition comprises 0.001-5% of the extract of Bombyx mori. 7. The cosmetic composition according to any one of claims 1 to 6, wherein the cosmetic material is a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing oil, powder foundation, emulsion foundation , A wax foundation, and a spray.

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