KR19990038744A - Method of extracting black beans and cosmetics containing black beans extract - Google Patents

Abstract

The present invention is to obtain an extract of black soybeans by immersing black soybeans in a solvent such as ethanol, butylene glycol, propylene glycol in order to obtain an extract having a skin improvement effect in black soybeans.

The fact that black beans are good for the skin is already known Baigi, there was no case of applying them as a cosmetic, the present invention is to extract the active ingredient having an improvement effect of the skin from black beans and to mix it into cosmetics to commercialize. Extract of the present invention is excellent in antioxidant activity, whitening effect, has the effect of skin improvement.

Description

Method of extracting black beans and cosmetics containing black beans extract

The present invention relates to skin cosmetics, and more particularly, to a method for extracting a liquid extract from black soybeans, having an excellent antioxidant effect and excellent stability, and a skin cosmetic containing the extract.

In general cosmetics, cosmetics containing raw materials extracted from natural products have been developed conventionally, such as ginseng extracts, yeast extracts, extracts such as pine mushroom extracts, natural polymers such as collagen, skin rejuvenating agents such as amino acids, vitamins or Moisturizers, such as glycerin and 1,3-butylene glycol, have been used in skin cosmetics for the purpose of preventing and improving skin damage, for example, and researches on techniques for adding active ingredients of natural substances to cosmetics have continued. have.

It is true that soy has high protein and fat content, which is high in edible value. Especially, black soybean has been a very important source of nutrition for people as a source of protein and fat. In addition, according to the herbal home remedies war, black soybean is a food that is very effective for making a good voice and good skin, and it has a record that it can be reproduced all year long. Also, black soybean is used for metabolism, asparagine, blood purification lecithin and It contains a lot of urease and various enzymes to enhance the function of the liver and kidneys. When this function of the liver and kidneys is strengthened, blood is purified and blood vessels are generated.

However, there has not been a report on the application of this directly to the skin as a cosmetic for black soybeans having such various effects and especially for improving the condition of the skin.

Accordingly, an object of the present invention is to provide a method for extracting an effective liquid component from black soybeans, which is excellent in preventing and improving various skin damages and in terms of stability.

Still another object of the present invention is to provide a cosmetic containing the liquid extract according to the present invention, which exhibits skin wrinkle prevention and antioxidant effects.

In order to achieve the above object, the present invention obtains a liquid extract by immersing black beans in a 50-90% ethanol aqueous solution extract of black beans or a mixture of 1, 3-butylene glycol and propylene glycol and water.

Black soybeans were used for farming in a common way in domestic farms, and black beans were completely ripe and dried for use as samples.

Liquid extract is applicable to cosmetics, wherein the content of the liquid extract of the present invention is preferably 0.05-30% by weight of the cosmetic.

Too little content is unlikely to produce an effect, and even when formulated more, the effect does not increase significantly.

The liquid extract of the present invention is easily blended in water and hardly blended in fats and oils and organic solvents, but can be blended into cosmetics even when suspended, and has no effect on the effect. When the liquid extract according to the present invention is blended into cosmetics, ordinary ingredients such as oil, water, surfactants, humectants, lower alcohols, thickeners, chelating agents, pigments, preservatives, etc. may be applied as necessary. It is possible to mix. In the present invention, the skin cosmetic is used for the skin, for example, may be in the form of a cosmetic, such as a lotion, latex, cream, pack, it is possible to apply to the skin anti-aging cosmetics.

Hereinafter, the present invention will be described in more detail with reference to Examples, but the scope of the present invention is not limited to the following Examples.

EXAMPLE

Black bean (genus name: Glycine max Merr) uses black beans grown generally in Korea, and ripened black beans are collected for use as samples and dried at room temperature until there is no change in weight.

As a solvent for extracting black beans, a solvent generally used in cosmetics may be used. In this embodiment, ethanol, 1,3-butylene, propylene glycol is used alone, or a mixture of these and water is used.

Extraction method is put 5-80% of black soybeans in each solvent and soak for at least 30 days at room temperature to 60 ℃, almost no active ingredient at temperature below room temperature, the extract is tangled by denaturation of components such as protein above 60 ℃ The phenomenon appears and results in undesirable.

After dipping, the filtrate is filtered through a 500 mesh filter, and the filtrate is filtered using a 0.45 micrometer filter paper and used as a sample.

The circulating temperature of the black soybeans at room temperature to 60 ° C circulating at daily intervals showed a higher effect of the extract than fixing the temperature.

Example 2

To 8 g of 95% ethanol, 0.05 g of polypyrrolidone, 0.1 g of oleyl alcohol, 0.2 g of polyoxyethyl lemonooleate, 0.2 g of fragrance, 0.1 g of paraoxybenzoic acid methyl ester, a small amount of antioxidant, a small amount of pigment Dissolve. 0.05 g of the liquid extract of Example 1 and 5 g of glycerine were dissolved in 85.33 g of purified water, and then the mixed solution was added and stirred to obtain a lotion having a skin improvement effect.

Example 3

1.2 g of cetyl alcohol, 10 g of squalene, 2 g of petrolatum, 0.2 g of paraoxybenzoic acid ethyl ester, 1 g of glycerin monoesteralide, 1 g of polyoxyethylene (20 mole added) monooleate, and 0.1 g of fragrance were dissolved by heating at 70 ° C. 0.5 g of the liquid extract of Example 1, 5 g of dipropylene glycol, 1500 g of polyethylene glycol, 0.2 g of triethanolamine, and 76.2 g of purified water were dissolved by heating to 75 ° C. Both are emulsified by mixing, followed by cooling to obtain an emulsion having a water / oil-based skin improvement effect.

Example 4

To 5 g of 95% ethyl alcohol, 1.2 g of polyoxyethylene sorbitan monooleate, 0.3 g of kitulose, 0.2 g of sodium hyaluronate, 0.2 g of vitamin E acetate, 0.2 g of sodium licorice, 0.1 g of paraoxybenzoic acid ethyl ester 1g of the liquid extract of Example 1 and a suitable amount of pigment are mixed to obtain a cosmetic liquid having an effect of skin improvement.

Test examples for determining the various effects of the liquid extract of black beans on the skin are as follows.

[Test Example 1]

This experiment is to measure the antioxidant effect for measuring the efficacy effect of the liquid extract according to the present invention.

Exam Outline:

In the present invention, the effect of removing the active oxygen produced by xanthine and xanthine oxidase by the extract according to the present invention, that is, the active oxygen effect, is evaluated to measure the antioxidant effect. The amount of active oxygen is measured by generating active oxygen by xanthine and xanthine oxidase, and reacting the activated oxygen with nitrobluetetrazolium (NBT) at a wavelength of 560 nm.

Test reagent:

A) 2.4 ml of 0.05 M Na ₂ CO ₃ buffer (molecular weight 105.99)

A solution of 5.25 g (50 mM) of sodium carbonate dissolved in purified water and a 50 mM NaHCO ₃ (molecular weight 84.01) solution were mixed to pH 10.2.

B) 0.1 ml of 3 mM xanthine solution

45.6 mg of xanthine (molecular weight 152.11) is dissolved in water to make 10 ml.

C) 0.1 ml of 3 mM EDTA solution

Sodium ethylenediamine tetraacetate (molecular weight 60.1) is dissolved in distilled water to make 3 mM.

D) 0.1 ml of 0.15% BSA solution

15 mg of BSA (Fration V, Powder, Sigma) is dissolved in distilled water and made 10 ml.

E) 0.1 ml of 0.75 mM NBT solution

61.32 mg of nitroblue tetrazolium (molecular weight 817.65) is dissolved in distilled water to make 10 ml.

F) 0.1 ml of xanthine oxidase solution

Dilute xanthine oxidase with distilled water approximately 100 times and allow the absorbance of the blank test to be within the range of 0.2-0.23 in the measurement procedure. Let the change in absorbance be A560 = 0.3 / 20 minutes.

G) 0.1 ml of 6mM copper chloride solution

102.29 mg of copper chloride (molecular weight 134.55) is dissolved in distilled water to make 100 ml.

How to measure:

1) Add B, B, C, D, and B to the Bayer bottle, add 0.1 ml of sample solution to this, and leave at 25 ℃ for 10 minutes.

2) Add a bar and stir quickly and incubate at 25 ° C for 20 minutes.

3) Then add the yarn to stop the reaction and measure the absorbance St at 560 nm.

4) In the blank test, absorbance Bt is measured by using distilled water instead of the sample solution as described above.

5) The blank test solution is operated in the same manner using distilled water instead of the bar to measure the absorbance Bo.

The antioxidant effect is calculated as follows.

% Inhibition = (1- (St-So) / Bt-Bo) × 100

St: absorbance at 560 nm after enzyme reaction of sample solution

Bt: absorbance at 560 nm after enzyme reaction of blank test solution

So: Absorbance at 560 nm before reaction without enzyme in sample solution

Bo: absorbance at 560 nm before reaction when enzyme free addition of blank test solution is added

The samples used in this experiment were all extracted and tested under the same conditions, and the average value of the experiments with the original sample and the sample prepared by the company, which were known to have high antioxidant effects, with the sample solution and 3% aqueous solution of each sample was averaged. Table 1 is a result of the antioxidant effect of each sample, the antioxidant effect was the greatest in the liquid extract of the present invention.

TABLE 1

[Test Example 2]

The cell proliferation effect of the liquid extract of the present invention was measured.

Test Methods:

In order to measure the cell proliferation effect of the liquid extract of the present invention, cell proliferation of mouse skin fibroblasts cultured by MTT reduction method was measured and this action was compared with other extracts.

This MTT reduction method utilizes the property of blue coloration when MTT is reduced by NADH present in intracellular mitochondria, and the amount of blue represents the amount and metabolic activity of mitochondria.

Reagents and test materials were used Eagle's MEM medium, fetal bovine serum (manufactured by Zipco Inc.), and MIDID reagent (Sigma). In addition, PBS reagents and other general reagents used a reagent express. Cells were NIH3T6, and for culturing, cells were incubated at 37 ° C. for 14 hours in a 5% FBS-containing MEM medium in 96-well plates to a density of 5 × 10 ³ cells / well, and 0.5% of each sample was added. After exchange with FBS and MEM medium, medium replacement again on day 3 and treated with MTT reagent to determine the difference in absorbance between 570nm and 620nm. At this time, the cell proliferation rate was determined by setting the absorbance difference of the blank to 100 and dividing the absorbance difference therefrom by the absorbance difference of the blank.

Test result:

Table 2 shows the cell proliferation rate when the concentration of each sample in the medium is 10%, and the cell proliferation effect of the liquid extract according to the present invention was superior to the Pracenta extract of the same concentration known to be excellent in cell proliferation effect.

TABLE 2

[Test Example 3]

The skin improvement effect of the liquid extract of the present invention was measured.

Test Sample:

The test sample is a cosmetic having different amounts of the liquid extract of Test Example 1, and the cosmetics used in the comparative experiments are creams whose compositions are shown in Table 3, and the phases listed in Table 3 are heated to 70 ° C. After preservation, a) phase is added, and after pre-emulsification, it is emulsified uniformly with a homomixer, and then it is cooled slowly to prepare a cream.

Subject:

5 women aged 20-35

Test Methods:

Each cream of Table 3 was applied to the right side of the subject's face twice a day for one consecutive month.

Result measurement:

After completion of the test, the skin of the application areas on both sides of the face was compared with the face color by an image analyzer, and the darkest color was set to 5, the middle color to 3, and the brightest color to 1.

Table 3 shows the compositions of Test Examples and Comparative Examples.

TABLE 3

Table 4 shows the results of comparing the facial skin color after applying the cream of Test Example B and the cream of Comparative Example to the subject's skin, respectively.

When the cream containing the extract of the present invention was applied, the skin color improvement effect was apparent, and the improvement effect was most apparent when the cream of Test Example A was applied, and the improvement was only slightly when the Cream of Test Example C was used. .

TABLE 4

As described above, the liquid extract according to the present invention has excellent anti-oxidant effect and cell proliferation effect, and has excellent skin color improvement effect and anti-aging effect when applied to the skin. In addition, since it can be applied to various skin cosmetics it can be commercialized by mixing in various cosmetics.

Claims (9)

Solvents commonly used in cosmetics alone or water and black water dried in a mixture of water and 5-80% by weight, immersed for at least 30 days, the extract was filtered through a 500 mesh filter paper and the filtrate was filtered with 0.45 micrometer filter paper. Extraction method of liquid extract, characterized in that the sterile filtration. The method of extracting a liquid extract according to claim 1, wherein the solvent generally used in cosmetics is selected from 50-95% ethanol extract, 1,3-butylene glycol and propylene glycol. The extraction method of a liquid extract according to any one of claims 1 to 2, wherein the extraction temperature is from room temperature to 60 ° C. Liquid extract useful for cosmetics obtained by the extraction method of any one of claims 1 to 3. A skin cosmetic comprising the liquid extract of claim 4 at 0.05 to 30% by weight. The skin cosmetic according to claim 5, wherein the skin cosmetic is in the form of a general cosmetic such as a cream, a lotion, an emulsion or a pack. 0.05 g of the liquid extract of claim 4, 5 g of glycerol was dissolved in 85.33 g of purified water, and this was 8 g of 95% ethanol, polypyrrolidone 0.05 g, oleyl alcohol 0.1 g, polyoxyethylene monooleate 0.2 g, fragrance 0.2 g, paraoxy A lotion obtained by mixing and stirring 0.1 g of benzoic acid methyl ester, a small amount of antioxidant, and a small amount of pigment. 0.5 g of the liquid extract of claim 4, 5 g of dipropylene glycol, 2 g of polyethylene glycol 1500, 0.2 g of triethanolamine, and 76.2 g of purified water were heated to 75 ° C. to dissolve it, 1.2 g of cetyl alcohol, 10 g of squalene, 2 g of vaseline, ethyl paraoxybenzoate 0.2 g of ester, 1 g of glycerin monoesteralide, 1 g of polyoxyethylene (20 mole added) monooleate, and 0.1 g of fragrance were mixed and melted at 70 ° C. to emulsify, and then cooled and obtained Skin fluids. 5 g of 95% ethyl alcohol, 1.2 g of polyoxyethylene sorbitan monooleate, 0.3 g of kitulose, 0.3 g of sodium hyaluronate, 0.2 g of vitamin E acetate, 0.2 g of sodium licorice, and paraoxy Cosmetic liquid obtained by mixing 0.1 g of ethyl benzoate and an appropriate amount of dye.