KR100604091B1 - Cosmetic Composition for Abirritating Skin Containing Extract of Black Soybean as Active Ingredient - Google Patents

Abstract

The present invention relates to a cosmetic composition for alleviating skin irritation, and more particularly, to a cosmetic composition for alleviating skin irritation containing black bean extract as an active ingredient. The black soybean extract of the present invention, in particular, supercritical fluid extract of black soybeans remarkably relieves skin irritation by cosmetic raw materials, and also increases inflammation inhibitory activity, and when used in cosmetics, has excellent skin irritation relieving effect.

Black soybean extract, supercritical fluid extraction, skin irritation relief, cosmetic composition

Description

Cosmetic composition for relieving skin irritation containing black bean extract as an active ingredient {Cosmetic Composition for Abirritating Skin Containing Extract of Black Soybean as Active Ingredient}

The present invention relates to a cosmetic composition for alleviating skin irritation, and more particularly, to a cosmetic composition for alleviating skin irritation containing black bean extract as an active ingredient.

Recently, the number of consumers who feel sensitive skin is increasing. In the survey of general consumers, 30-40% of respondents said they were sensitive skin, and this rate is increasing. The increasing rate of sensitive skin is due to the increase in ultraviolet radiation due to the destruction of the ozone layer, the drying of the living space, the constitution of allergies, stress, and the increase of chemical harmful substances. On the other hand, the recent shift toward consumer-friendly and hypoallergenic consumerism has also been suggested to be a factor in improving awareness of sensitive skin. Accordingly, interest in substances that alleviate skin irritation caused by cosmetics is increasing. It is rising.

In general, various raw materials are used to make cosmetics, and typically 20 to 50 kinds of raw materials are used. These ingredients have been proved to be safe through skin irritation tests, but when applied to the product they can cause irritation such as inflammation, itching and allergies on the skin. However, these stimulus reactions do not happen to everyone, and may only happen to certain people.

Skin irritation that may occur with the use of cosmetics includes irritation such as itching, stinging and burning, and erythema and edema. Contact dermatitis includes irritant contact dermatitis, allergic contact dermatitis, sensory irritation, and phototoxic dermatitis, but it is difficult to clearly distinguish them, and most of them are mixed with each other.

First of all, in order for skin irritation to occur, skin penetration by cosmetics must first occur, and they interact with the epidermis or dermal cells to activate the immune system and cause irritation. In order to alleviate such skin irritation, various methods, such as a method of removing skin irritants, have been mobilized, but functional cosmetics have recently been increasing.

Therefore, cosmetics manufacturers are making efforts to minimize the irritation of the cosmetic raw material by applying a commonly used irritant substance to the product. However, this study is limited because the functional raw material itself often acts as a stimulus source. Therefore, it is necessary to select a substance that can reduce side effects caused by cosmetics and alleviate skin irritation while maintaining the effects of functional raw materials.

Throughout this specification many patent documents and articles are referenced and their citations are indicated. The disclosures of cited patent documents and articles are incorporated herein by reference in their entirety, and the level of the technical field to which the present invention belongs and the gist of the present invention are more clearly explained.

The present inventors have diligently tried to solve the above-mentioned needs of the art, and found that black bean extract, in particular, black bean extract prepared by supercritical fluid extraction, has an excellent anti-irritant effect and anti-inflammatory effect from stimulants. When used in cosmetics, it was confirmed that the effect of relieving skin irritation is excellent, and completed the present invention.

Accordingly, it is an object of the present invention to provide a cosmetic composition for skin irritation relief containing black bean extract as an active ingredient.

Other objects and advantages of the present invention will become apparent from the following detailed description and claims.

According to an aspect of the present invention, the present invention provides a cosmetic composition for alleviating skin irritation containing black soybean extract as an active ingredient.

The present inventors prepared extracts by various extraction methods using a plurality of natural products as a raw material, the black bean extract extracted by the solvent extraction method, as a result of performing the work of selecting a substance excellent in stimulating cell stimulation and inflammation inhibition from the stimulating source, In particular, it has been found that black bean extracts prepared by supercritical fluid extraction are most suitable for the purposes of the present invention described above.

On the other hand, according to the disclosure of Korean Patent Application No. 1997-58585, it is known that there is an antioxidant, cell proliferation effect and skin color improvement effect when extracting the water-soluble component from the black soybean by a general solvent extraction method and used as a cosmetic component. However, the black bean extract of the present invention has a novel effect of stimulating the cell stimulation and anti-inflammatory effect from the stimulus, and found that the effect of the supercritical fluid extract of black beans is very excellent.

The black bean extract contained in the cosmetic composition of the present invention may be prepared according to a conventional method known in the art, that is, using a conventional solvent, under conditions of conventional temperature and pressure.

The extraction solvent used to prepare the black bean extract of the present invention is water, anhydrous or hydrous lower alcohol having 1 to 4 carbon atoms, a mixed solvent of the lower alcohol and water, acetone, ethyl acetate, chloroform, 1,3-butyl It is preferable that it is selected from the group which consists of len glycol, hexane, and diethyl ether, and water is more preferable.

According to a preferred embodiment of the present invention, the black bean extract contained in the cosmetic composition of the present invention is prepared by using supercritical fluid extraction instead of the conventional organic solvent extraction method.

Supercritical fluids have the properties of liquids and gases when the gas reaches a critical point at high temperature and high pressure and have a chemically similar polarity to nonpolar solvents ( J. Chromatogr ., 479: 200-205 (1998)). Due to these properties, supercritical fluids are used to extract fat-soluble substances. Examples of extraction of fat-soluble substances using supercritical fluids include extraction of petroleum hydrocarbon compounds and polychlorine compounds from soil ( J. Chromatogr.A. , 845: 357-363 (1999)), extraction of aromatic hydrocarbon compounds from edible fats ( J. Chromatogr. A., 882: 245-253 (2000)), extraction of fat-soluble vitamins from milk lipid components ( J. Chromatogr . A., 874: 275-283 (2000)).

Black bean supercritical fluid extracts of the present invention may be prepared according to conventional methods known in the art, i.e., using conventional solvents, under the conditions of conventional temperature and pressure applied for supercritical fluid extraction.

According to a preferred embodiment of the present invention, the supercritical fluid extraction method used in the present invention is a method capable of effectively extracting fat-soluble substances by using supercritical carbon dioxide. In the present invention, the carbon dioxide becomes a supercritical fluid having both liquid and gaseous properties through the operation of the supercritical fluid apparatus to the pressure and temperature to the critical point, and as a result, the solubility in the fat-soluble solute increases. When supercritical carbon dioxide passes through an extraction vessel containing a certain amount of sample, the fat-soluble substance contained in the sample is extracted from the supercritical carbon dioxide.

After extracting the fat-soluble substance, the supercritical carbon dioxide containing a small amount of cosolvent is passed through the sample remaining in the extraction container to extract components that were not extracted with pure supercritical carbon dioxide alone. Preferably, the active ingredient can be effectively extracted by using supercritical carbon dioxide containing a small amount of cosolvent from the beginning.

Most of the extracted samples contain carbon dioxide. Since carbon dioxide is volatilized into air at room temperature, the extract obtained by the above method may be used as a cosmetic composition, and the cosolvent may be removed by a reduced pressure evaporator.

More specifically, the black bean supercritical fluid of the present invention is prepared by a process comprising the following steps: (a) adding black beans to a supercritical extraction vessel and carbon dioxide at a temperature of 35 to 100 ° C. and 100 to 500 atmospheres. Flowing the extraction vessel in a supercritical state; And (b) obtaining a component dissolved in the supercritical carbon dioxide flowing in step (a).

According to a specific embodiment of the present invention, the above-described extraction process is carried out as follows: First, black beans, which are the raw materials for extraction, are washed with purified water, dried and broken into small pieces, and then placed in a supercritical extraction container. Black beans as the material for extraction may be dried or used as it is, preferably dried and used. As a drying method, natural drying or a hot air drying method can be used.

Subsequently, pure carbon dioxide is supercritical at a suitable temperature and pressure using a liquid chromatography pump and flows into the extraction vessel. Or co-solvent is flowed with carbon dioxide. The temperature condition for making the carbon dioxide into the supercritical state is preferably 35 to 100 ° C, more preferably 50 to 70 ° C, most preferably about 60 ° C. In addition, the pressure condition for making carbon dioxide into a supercritical state is preferably 100 to 500 atmospheres, more preferably 200 to 400 atmospheres, and most preferably 300 atmospheres.

When supercritical carbon dioxide is flowed into the extraction container, preferably, a co-solvent is further mixed and flowed out. That is, a mixed fluid composed of supercritical carbon dioxide and a cosolvent is used. The cosolvent is preferably selected from the group consisting of ethanol, methanol, water, ethyl acetate, hexane and diethyl ether, more preferably ethanol, even more preferably 80% to 100% ethanol, most preferred Preferably about 95% ethanol. The mixed fluid contains 3 to 40% by volume of cosolvent, most preferably about 10% by volume, based on the total volume.

Then, a component dissolved in the supercritical carbon dioxide (mixed fluid in the case of using a co-solvent) flowing in the step (a) is obtained. Subsequently, the obtained extract is dried with a rotary evaporator to obtain a black soybean supercritical fluid extract powder.

On the other hand, the black bean extract of the present invention includes not only the extract by the above-described extraction method, but also an extract that has undergone a conventional purification process. Obtained by various additional purification methods, such as, for example, separation using ultrafiltration membranes having a constant molecular weight cut-off value, separation by various chromatography (manufactured for separation according to size, charge, hydrophobicity or affinity). The fraction is also interpreted to be included in the black bean extract of the present invention.

Black bean extract of the present invention may be prepared in a powder state by an additional process such as distillation under reduced pressure, freeze drying or spray drying.

According to a preferred embodiment of the present invention, the content of the extract is 0.0001-10.0% by weight relative to the total weight of the cosmetic composition. If the content is less than the expected effect is difficult to appear, if the content is exceeded the effect is no longer increased.

The cosmetic composition of the present invention is prepared as a composition comprising a black bean extract, preferably a black bean supercritical fluid extract, as an active ingredient, and may be applied before or after applying a cosmetic composition having a large skin irritation. In addition, black bean extract, preferably black bean supercritical fluid extract may be added to the skin by additionally added to the cosmetic composition with a high skin irritation.

Cosmetic compositions with high skin irritation are lactic acid, glycolic acid, salicylic acid or retinoic acid, and preferably lactic acid. Therefore, according to another aspect of the present invention, the present invention provides a cosmetic composition comprising the black soybean extract and lactic acid as a skin irritant.

The ingredients included in the cosmetic composition of the present invention include ingredients commonly used in the composition in addition to the black bean extract as active ingredients, and include, for example, conventional auxiliaries such as antioxidants, stabilizers, solubilizers, vitamins, pigments and flavors, and Carrier.

The compositions of the present invention may be prepared in any formulation conventionally prepared in the art, for example, solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, surfactant-containing cleansing, It may be formulated as an oil, powder foundation, emulsion foundation, wax foundation, spray, and the like, but is not limited thereto. More specifically, when the composition of the present invention is used as a cosmetic composition, it is prepared in the form of a flexible lotion, nutrition lotion, nutrition cream, massage cream, essence, eye cream, cleansing cream, cleansing foam, cleansing water, pack, spray or powder Can be.

When the formulation of the present invention is a paste, cream or gel, animal oils, vegetable oils, waxes, paraffins, starches, trachants, cellulose derivatives, polyethylene glycols, silicones, bentonites, silicas, talc or zinc oxide may be used as carrier components. Can be.

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used, in particular in the case of a spray, additionally chlorofluorohydrocarbon, propane Propellant such as butane or dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, solubilizer or emulsifier is used as the carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 Fatty acid esters of, 3-butylglycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan.

When the formulation of the present invention is a suspension, liquid carrier diluents such as water, ethanol or propylene glycol, suspending agents such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, microcrystals Soluble cellulose, aluminum metahydroxy, bentonite, agar or tracant and the like can be used.

When the formulation of the present invention is a surfactant-containing cleansing, the carrier component is aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide. Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters and the like can be used.

The cosmetic composition containing the black bean extract of the present invention, preferably the black bean extract prepared by the supercritical fluid extraction method, greatly reduces skin irritation by the cosmetic raw material, suppresses inflammation developed by skin irritation, and stabilizes the formulation. outstanding.

Hereinafter, the present invention will be described in more detail with reference to Examples. These examples are only for illustrating the present invention in more detail, and it will be apparent to those skilled in the art that the scope of the present invention is not limited by these examples according to the gist of the present invention. .

Preparation Example 1 Preparation of Black Bean Extract Using Supercritical Fluid Extraction Method

Black beans (Bioland, Korea) were washed with purified water, dried and broken into small pieces, and then 1 g of the sample was placed in a supercritical fluid extractor (Jasco, Japan) and a liquid chromatography pump (Supercritical fluid chromatograph, Jasco). , Japan) using pure carbon dioxide in a supercritical state at 60 ℃ and 300 atm conditions and 10% by volume of 95% alcohol was mixed and flowed to 40 minutes every 4 minutes while receiving the extract in a 10 ml glass test tube. For supercritical extraction in preliminary experiments, 60 ° C. and 300 atm showed the best results, and this condition was used in the present invention. The extract was dried with a rotary evaporator to obtain an extract having a dry weight of 51.4 mg / g. The dried black bean extract powder was used dissolved in 50% 1,3-butylene glycol.

Preparation Example 2 Preparation of Black Bean Extract Extracted Using Water as a Solvent

After washing with purified water, 1 kg of dried black beans (Bioland, Korea) was placed in 5 l of purified water, and extracted by heating at 70-90 ° C. for 5 hours in an extractor equipped with a cooling condenser, followed by filtration with 300 mesh filter cloth, at 5-10 ° C. After aging for 7-10 days, it was filtered through Whatman No. 5 filter paper. The filtrate was dried at 70 ° C. with a rotary evaporator to obtain an extract having a dry weight of 58.5 g.

Experimental Example 1: Relaxation effect of cell stimulation by lactic acid

In order to confirm that the black soybean extract of the present invention has a skin stimulating effect, a cell stimulation relaxation experiment with lactic acid was performed. Fibroblasts (Korea Cell Line Bank), which are human skin cells, were cultured in T-75 flasks (Falcon, USA) until 80% growth. The cultured cells were transferred again to 3 × 10 ⁴ cell counts / well in each well of a 96-well plate (Falcon, USA) and incubated for 24 hours. After incubation, the cells were completely attached and grown well under a microscope, and then experiments were performed using lactic acid, a skin cell stimulant. At this time, lactic acid was used at a concentration of 0.2% that kills all skin cells. That is, 200 μl of DMEM (Sigma, United States) medium containing 0.2% lactic acid was added to each well of a 96 well-plate, and the supercritical fluid extract of black soybean obtained in Preparation Example 1 and the water of black soybean obtained in Preparation Example 2 Extracts were added at 100, 200, 500 and 1000 μg / ml concentrations. At this time, it was used as a control group that did not add all the lactic acid and extract, the comparison group was added only lactic acid. Twelve μl of MTT ([3- (4,5-dimethylthiazol-2-yl) -2,5-diphenyltetrazolium bromide) reagent was added to each well to compare the viability of the cells 12 hours after the addition of the test substance. .

After 4 hours with the addition of the MTT reagent, the absorbance was measured at 570 nm using an ELISA Reader. Cell viability was calculated according to Equation 1 using the measured absorbance values.

% Cell viability = [(control absorbance-test substance absorbance) / control absorbance] × 100

Cell viability Concentration of extract (µg / ml) Cell survival rate (%) Supercritical Fluid Extract of Black Beans Black Bean Water Extract Comparison Control 100 29.4 11.8 0.0 100.0 200 42.6 23.9 0.0 100.0 500 58.3 36.8 0.0 100.0 1000 73.9 59.2 0.0 100.0

As shown in Table 1, when the water extract of black soybean and the supercritical fluid extract of black soybean were treated, the cell viability increased depending on the treatment concentration, and in particular, the cell viability of the black soybean extract obtained by supercritical fluid extraction was much higher. .

Experimental Example 2: anti-inflammatory effect-carrageenan edema experiment

Carrageenan foot edema (Carrageenan Foot Edema) experiment was carried out to determine whether the black bean extract of the present invention exhibits an inhibitory effect. The supercritical fluid extract of black soybean obtained in Preparation Example 1 and the water extract of black soybean obtained in Preparation Example 2 were each administered 10-100 mg / kg into the abdominal cavity of rats. One hour after the administration of the extract, 0.5 ml of 0.1% carrageenan solution (Sigma, United States of America) was injected into the hind paw of the experimental animal to induce inflammation. Immediately after carrageenan injection and 4 hours after injection, the volume change of the rat's foot was measured. The edema inhibition rate was calculated according to Equation 2 using the measured volume change of the foot, and the results are shown in Table 2 below.

% Inhibition = (1-ΔV _{treated group} / ΔV _control ) × 100

ΔV is the volume change of the foot.

Anti-inflammatory effect Extract dose (mg / kg) % Inhibition Rate Supercritical Fluid Extract of Black Beans Black Bean Water Extract 10 39.4 26.2 30 47.6 33.8 50 59.7 48.3 100 78.1 56.5

As shown in Table 2, the black bean extract increased the inhibition rate of inflammation depending on the dose. In particular, supercritical fluid extracts of black soybean showed much better anti-inflammatory effects.

Experimental Example 3: skin irritation relief effect-human skin patch test

When the black soybean extract of the present invention is directly applied to human skin, human skin patch test was carried out to check whether the black bean extract has a skin irritation-reducing effect. The subjects of this study were 20 women in their 20s and 30s who had no history of hypersensitivity to skin irritation and have no skin disease or skin allergy. To the nutritional cream formulation of Formulation Example 4, lactic acid, a skin irritant, was added 5.0% as an irritant, and 2% supercritical fluid extract of black soybean obtained in Preparation Example 1 (Example 1) and water of black soybean obtained in Preparation Example 2 2% extract (Example 2) was added to the test material. At this time, the addition of both lactic acid and extract was used as a control example, the addition of only lactic acid was used as a comparative example.

The test site was first wiped with 70% ethanol and then dried. 15 μg of the prepared test substance was added dropwise into a Fin chamber (Finn chamber, 100 × 10, EPITEST, Finland), and then sealed in the forearm inner part of the test subject. The patch was applied for 24 hours, the patch was removed, and the test site was marked with a marker pen. After 1 hour and 24 hours (1 hour after removal of the patch, after 24 hours = 24 hours, 48 hours including patch attachment), the presence of erythema and edema at the test site was observed using a magnifying glass (8MC-150, DAZOR, United States of America). .

Skin response was determined according to the rules of the International Contact Dermatitis Research Group (ICDRG) (Table 3), the average skin reactivity was calculated by the following equation 3 and the results are shown in Table 4.

Evaluation criteria and score of skin reaction sign score Evaluation standard - 0 No response ± 0.5 Faint or light erythema + One Boundary but weak erythema, edema and papules ++ 2 Pronounced erythema, papules and vesicles +++ 3 Severe erythema and alveolar, scleroderma

Mean skin responsiveness (mean score) = {(score × number of respondents) / (maximum score × total number of subjects)} × 100

Test substance 24 hours 48 hours Average skin reactivity (n = 20)  ± + ++  ± + ++ Control  3-- --- 1.25 Comparative example 10 2- 2 1- 7.50 Example 1  6 1- One - - 3.75 Example 2  7 2- 1 1- 5.83

As can be seen in Table 4, the formulation containing the black bean extract (Examples 1 and 2) for the skin irritation induced by lactic acid showed a skin irritation-reducing effect, in particular containing a supercritical fluid extract of black beans The relieving effect of the skin irritation of the formulation (Example 1) was excellent.

Experimental Example 4: The effect of relieving skin irritation when applied to the human body

Twenty females in their 20s and 30s were allowed to use the formulation of Example 1, Example 2, Control and Comparative Example used in Experiment 3 for 10 days. Each morning and evening were used, and then questionnaires were marked for skin irritation and intensity. The test results were evaluated once a day, and divided into subjective and objective stimuli.

Test substance Score × Response subjects Subjective stimulation Objective stimulation Sum Control 3 One 4 Comparative example 37 22 59 Example 1 13 10 23 Example 2 20 14 34

As can be seen in Table 5, the formulation containing the black bean extract (Examples 1 and 2) alleviated both subjective and objective stimuli of skin irritation induced by lactic acid, in particular supercritical fluid extract of black beans It was found that the stimulating alleviation effect of the containing formulation (Example 1) was excellent.

Experimental Example 5: Stability Test of the Formulation

In order to confirm the stability of the cosmetic formulation containing the black soybean extract as an active ingredient, the stability according to the temperature change was tested. Formulations containing supercritical fluid extracts of black beans (Example 1), formulations containing water extracts of black beans (Example 2) and control formulations containing no black bean extracts were kept constant at 45 ° C. and 4 After storing for 12 weeks in an opaque glass jar in a fully shaded refrigerator kept constant at ℃ ℃, the degree of separation and discoloration was measured. The degree of separation and discoloration of the formulations were evaluated by classifying them into six grades according to the following discoloration evaluation criteria.

<Evaluation criteria of discoloration of formulation>

0: no change 1: very little separation (discoloration)

2: little separation (discoloration) 3: little separation (discoloration)

4: severely separated (discolored) 5: extremely severely separated (discolored)

Temperature Degree of discoloration of the formulation Example 1 Example 2 Control 45 ℃ 0 0 0 0 ℃ 0 0 0

As shown in Table 6, it was found that the cosmetic compositions of Examples 1 and 2, the control examples do not discolor and separate, so there is no abnormality in the stability of the cosmetic formulation to which the black soybean extract was added.

On the basis of the above experimental examples, a cosmetic composition of various solubilizers and emulsifiers containing water extract of black soybean or supercritical fluid extract of black soybean having excellent skin irritation-reducing effect is presented. 'Black bean extract' described in the formulation example means water extract of black beans or supercritical fluid extract of black beans. Although this is described in detail in the following formulation example, the composition of the present invention is not limited only to the following examples.

Formulation Example 1: Softener (Skin Lotion)

A flexible longevity containing the black soybean extract as shown in Table 7 below was prepared according to a conventional method.

ingredient Content (% by weight) Black Bean Extract 1.0 glycerin 5.0 1,3-butylene glycol 3.0 PEG 1500 1.0 Allantoin 0.1 DL-panthenol 0.3 EDTA-2Na 0.02 Benzophenone-9 0.04 Sodium hyaluronate 5.0 ethanol 10.0 Octicdodeceth-16 0.2 Polysorbate 20 0.2 incense 0.02 Uniside-U 13 0.01 Green # 13 0.001 Distilled water Remaining amount Sum 100

Formulation Example 2: Converging Cosmetic Water

As shown in Table 8 below, astringent cosmetic water containing black bean extract was prepared according to a conventional method.

ingredient Content (% by weight) Black Bean Extract 1.0 glycerin 2.0 1,3-butylene glycol 2.0 Allantoin 0.2 DL-panthenol 0.2 EDTA-2Na 0.02 Benzophenone-9 0.04 Sodium hyaluronate 3.0 ethanol 15.0 Polysorbate 20 0.3 Witch Hagel Extract 2.0 Citric acid a very small amount incense 0.02 Uniside-U 13 0.01 Green # 13 0.001 Distilled water Remaining amount Sum 100

Formulation Example 3: Nutrients

Nutrients containing black soybean extract as shown in Table 9 below were prepared according to a conventional method.

ingredient Content (% by weight) Black Bean Extract 1.0 Glyceryl Stearate SE 1.5 Cetearyl Alcohol 1.5 lanolin 1.5 Polysorbate 60 1.3 Sorbitan stearate 0.5 Cured Vegetable Oil 1.0 Mineral oil 5.0 Squalane 3.0 Trioctanoine 2.0 Dimethicone 0.8 Tocopherol Acetate 0.5 Carboxyvinyl Polymer 0.12 glycerin 5.0 1,3-butylene glycol 3.0 Sodium hyaluronate 5.0 Triethanolamine 0.12 incense 0.01 Uniside-U 13 0.02 Green # 3 0.003 Distilled water Remaining amount Sum 100

Formulation Example 4: Nutrition Cream

Nutritional cream containing a black bean extract as shown in Table 10 below was prepared according to a conventional method.

ingredient Content (% by weight) Black Bean Extract 3.0 Lipophilic monostearate 2.0 Cetearyl Alcohol 2.2 Stearic acid 1.5 Beeswax 1.0 Polysorbate 60 1.5 Sorbitan stearate 0.6 Cured Vegetable Oil 1.0 Squalane 3.0 Mineral oil 5.0 Trioctanoine 5.0 Dimethicone 1.0 Sodium magnesium silicate 0.1 glycerin 5.0 Betaine 3.0 Triethanolamine 1.0 Sodium hyaluronate 4.0 incense 0.01 Uniside-Y13 0.02 Green # 3 0.003 Distilled water Remaining amount Sum 100

Formulation Example 5: Massage Cream

A massage cream containing a black bean extract was prepared according to a conventional method as shown in Table 11 below.

ingredient Content (% by weight) Black Bean Extract 2.0 Lipophilic Monostearic Acid Glycerin 1.5 Cetearyl Alcohol 1.5 Stearic acid 1.0 Polysorbate 60 1.5 Sorbitan stearate 0.6 Isostearyl Isosterate 5.0 Squalane 5.0 Mineral oil 35.0 Dimethicone 0.5 Hydroxyethyl cellulose 0.12 glycerin 6.0 Triethanolamine 0.7 incense 0.01 Uniside-Y13 0.02 Green # 3 0.003 Distilled water Remaining amount Sum 100

Formulation Example 6: Essence

Essences containing the black bean extract as shown in Table 12 below were prepared according to a conventional method.

ingredient Content (% by weight) Black Bean Extract 2.0 glycerin 10.0 Betaine 5.0 PEG 1500 2.0 Allantoin 0.1 DL-panthenol 0.3 EDTA-2Na 0.02 Benzophenone-9 0.04 Hydroxyethyl cellulose 0.1 Sodium hyaluronate 8.0 Carboxy Vinyl Polymer 0.2 Triethanolamine 0.18 Octyldodecanol 0.3 Octyldodeceth-16 0.4 ethanol 6.0 incense 0.02 Uniside-U 13 0.01 Green # 3 0.001 Distilled water Remaining amount Sum 100

Formulation Example 7: Pack

As shown in Table 13 below, a pack containing black bean extract was prepared according to a conventional method.

ingredient Content (% by weight) Black Bean Extract 1.0 Polyvinyl alcohol 15.0 Cellulose gum 0.15 glycerin 3.0 PEG 1500 2.0 Chicdestrin 0.15 DL-panthenol 0.4 Allantoin 0.1 Glycyrisin Monoammonium 0.3 Nicotinamide 0.5 ethanol 6.0 PEG 40 Cured Castor Oil 0.3 incense a very small amount Uniside-Y13 0.02 Green # 3 0.003 Distilled water Remaining amount Sum 100

As described above, the black bean extract, preferably the black bean extract prepared by the supercritical fluid extraction method remarkably relieves skin irritation, has anti-inflammatory activity, and when used in cosmetics, has excellent skin irritation effect. Therefore, the black bean extract of the present invention is useful as a cosmetic composition for relieving skin irritation that may occur when the cosmetic is applied to the skin.

Having described the specific part of the present invention in detail, it is apparent to those skilled in the art that the specific technology is merely a preferred embodiment, and the scope of the present invention is not limited thereto. Thus, the substantial scope of the present invention will be defined by the appended claims and equivalents thereof.

Claims (7)

Cosmetic composition for skin irritation relief containing black bean extract as an active ingredient. The cosmetic composition for alleviating skin irritation according to claim 1, wherein the black soybean extract is prepared by a supercritical fluid extraction method. The cosmetic composition for alleviating skin irritation according to claim 2, wherein the black bean extract uses supercritical carbon dioxide as a solvent. The cosmetic composition for alleviating skin irritation according to claim 3, wherein the black bean extract uses supercritical carbon dioxide as a solvent and ethanol as a cosolvent. The cosmetic composition for alleviating skin irritation according to claim 1, wherein the content of the black bean extract is 0.0001-10% by weight based on the total weight of the composition. The cosmetic composition for alleviating skin irritation according to claim 1, wherein the composition further comprises lactic acid. The group of claim 1 wherein the composition consists of a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation and spray Cosmetic composition for skin irritation relief, characterized in that it has a formulation selected from.